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1.
Two Exobasidium species causing Exobasidium leaf blister on Rhododendron spp. are described. An Exobasidium leaf blister on Rhododendron yedoense var. yedoense f. yedoense has been recognized in Hokkaido Prefecture, Japan, since the first report was issued in 1950. The causal fungus is identified
with Exobasidium dubium from the morphology of its hymenial structure and mode of germination of the basidiospores. Another Exobasidium leaf blister
on Rhododendron dauricum has been observed in Hokkaido Prefecture, Japan. In comparison with morphology based on hymenial structure and mode of germination
of the basidiospores of the 100 validly described taxa, this fungus differs from those known taxa in the size of basidia and
basidiospores, the numbers of sterigmata and septa of basidiospores, and the mode of germination of basidiospores. Thus, a
new species, Exobasidium miyabei, is established and illustrated.
Received: February 13, 2002 / Accepted: September 25, 2002
Present address: National Institute of Agrobiological Sciences, Tsukuba 305-8602, Japan
Acknowledgments We profoundly appreciate the cooperation of Dr. V. Melnik in providing Russian papers and Dr. L. Vasilyeva for translating
them into English. We thank Prof. H. Takahashi for loaning the materials in the Herbarium of the Hokkaido University Museum
and Dr. W. Abe, Graduate School of Science, University of Hokkaido, for his kind help with the sampling of R. dauricum in Teshikaga, Hokkaido Prefecture. This study was supported in part by a Grant-in-Aid for Scientific Research (B) (No. 13460019),
Japan Society for the Promotion of Science (JSPS).
Contribution No. 171, Laboratory of Plant Parasitic Mycology, Institute of Agriculture and Forestry, University of Tsukuba.
Correspondence to:M. Kakishima 相似文献
2.
3.
A new species of Erysiphe sect. Uncinula is described and illustrated from Patagonia, Argentina. Erysiphe patagoniaca sp. nov., found on leaves of Nothofagus × antarctica, is similar to E. nothofagi and E. kenjiana, but differs in its appendages being twisted throughout their length and the number of appendages, asci, and ascospores.
The two endemic species of Erysiphe sect. Uncinula, E. magellanica and E. nothofagi, coexisted on the same leaves together with Erysiphe patagoniaca.
Received: September 19, 2002 / Accepted: November 28, 2002
Acknowledgments The authors are grateful to Ms. Seiko Niinomi for providing the micrographs of ascomata of Erysiphe spp. on Nothofagus.
Correspondence to:S. Takamatsu 相似文献
4.
Interactions among granivores and seeds depend on the foraging behaviour and morphology of the granivores and on the attributes
and availability of seeds. We investigated seed selection by the seed harvesting ant Messor bouvieri in three adjacent plant communities in Spain by relating the harvested seeds to those in the seed rain. Preference was positively
correlated with seed size and abundance which accounted for 43% and 20% of the variance respectively. Contrary to predictions
of central place foraging theory, the size of seeds harvested did not increase with distance from the nest. Inclusion of a
less-preferred item in the diet was more strongly related to the abundance of more-preferred items (60% of the variance) than
the abundance of the less-preferred item (14% of the variance). Worker size accounted for 20–30% of the variance in the size
of harvested seeds, although small workers did not appear to be constrained by load size for the range of seeds available.
The body size of ants was significantly larger in the community with the greatest proportion of large seeds, although this
was not due to their ability to carry larger loads or due to the greater force required to crush these seeds. The strong preference
of M. bouvieri for large seeds may have important consequences for the plant communities in which they forage.
Received: 30 November 1999 / Accepted: 14 April 2000 相似文献
5.
We identified the principal factors causing losses in reproductive potential (i.e. quantity of potentially dispersable seeds)
over the predispersal period of the reproductive cycle of Sorbus aucuparia L. (Rosaceae) in various populations in the NW Iberian Peninsula. Over the period 1999–2002 we examined the magnitude and
variability of losses observed in each stage from bud production to seed production (i.e. bud to flower, flower to initiated
fruit, initiated to full-size fruit, and full-size to mature fruit). We also investigated spatial and temporal variation in
these losses. Reproductive potential showed very significant losses over the predispersal period in all 4 years: in no case
did the number of mature fruits available for dispersal exceed 20% of the initial number of flower buds. In all 4 years of
study, we detected statistically significant variation among populations in total losses. The principal cause of loss of reproductive
potential over the predispersal period in our Sorbus aucuparia populations was non-initiation of fruit. The plant’s interactions with predispersal predators of buds and seeds show high
spatial and temporal variability. Also, the proportions of loss due to the different components did not show within-individual
consistency over time. It thus seems unlikely that the different factors contributing to predispersal losses in rowan are
currently generating significant selective pressures in our study populations. 相似文献
6.
Ophiodothella caseariae sp. nov. from leaves of Casearia tremula in Venezuela is described and illustrated.
Received: February 19, 2002 / Accepted: April 30, 2002 相似文献
7.
B incompatibility factor mutants (Bmut) in Pleurotus ostreatus were recovered from common-B mating heterokaryons resulted from matings between wild-type monokaryons with different A but the same B factors (A1B2 and A2B2) after NTG mutagenesis. The mutant monokaryons such as A1B2mut and A2B2mut were observed to have regularly uninucleated hyphal cells and to be compatible with each other. Matings between A1B2mut and A2B2mut monokaryons produced stable heterokaryons (A1B2mut + A2B2mut) that had binucleated hyphal cells with true clamp connections and formed normal fruit-bodies. Mating tests using basidiospore
progeny from each of these heterokaryons revealed the bipolar mating pattern. Genetic analysis suggested that the mutation
of B factor in P. ostreatus might occur in the B incompatibility factor genes.
Received: August 3, 2001 / Accepted: January 18, 2002 相似文献
8.
Umbelopsis gibberispora is described as a new species in the genus Umbelopsis, Umbelopsidaceae, Mucorales. The species differs from others in this genus by ellipsoidal sporangiospores with unilaterally
thickened walls. Phylogenetic analyses based on nuclear large subunit ribosomal DNA (nLSU rDNA) partial sequences suggest
that U. gibberispora, U. swartii, and U. westeae form a clade together with the strains of Umbelopsis ramanniana. The ex-type strain of Micromucor ramannianus var. angulisporus is found to be very close to Umbelopsis vinacea, whereas other isolates identified under the former name in the sense of Linnemann fall in the U. ramanniana subclade. For these isolates, a new species, Umbelopsis angularis, is introduced. Phylogenetic relationships among Umbelopsis species are discussed related to their attributes of the sporangial wall and mature spore shapes.
Received: August 27, 2002 / Accepted: March 11, 2003
Acknowledgments We thank Dr. Takashi Ohsono, Graduate School of Agriculture, Kyoto University, Japan, for providing the strain of U. gibberispora (CBS 109328). We also thank Dr. Wieland Meyer, University of Sydney, Australia for access to the phylogenetic tree based
on ITS sequence data before publishing, and Dr. Richard C. Summerbell, Centraalbureau von Schimmelcultures, the Netherlands,
for linguistic corrections. 相似文献
9.
Chlamydospores of Entoloma clypeatum f. hybridum were described on the mycorrhizas and rhizomorphs associated with Rosa multiflora. Their developmental pattern seems to be the Nyctalis type. This is the first report on chlamydospore formation on the mycorrhizae in entolomatoid fungi.
Received: January 17, 2002 / Accepted: November 5, 2002
Acknowledgments K.H. is grateful to Emeritus Professor N. Sagara in Kyoto University, in whose laboratory part of this study was undertaken.
Thanks are due to Mr. D. Sakuma for allowing the specimens to be kept in Osaka Museum of Natural History.
Correspondence to:H. Kobayashi 相似文献
10.
11.
H. Takahashi 《Mycoscience》2003,44(2):0103-0107
Three new species of Agaricales from eastern Honshu, Japan, are described and illustrated. (1) Clitocybe minutella sp. nov. (section Vernae), having white, very small, clitocyboid basidiomata with white, strigose mycelial tomentum at the base of the stipe and trichodermial
elements in the pileipellis, occurs on leaf litter in deciduous oak forests. (2) Crepidotus longicomatus sp.nov. (section Echinospori), forms very small (up to 3.5 mm in diameter), reniform basidiomata densely covered overall with white to pale yellow, erect,
thick-walled, long hairs. It has melleous, echinulate basidiospores, and was found on fallen dead branches in lowland oak
forests. (3) Crepidotus virgineus sp. nov. (section Crepidotus), forming pure white, reniform basidiomata, has smooth, ellipsoid basidiospores, subfusiform-pedicellate to irregularly cylindrical
cheilocystidia, and abundant clamp connections. It occurs on fallen dead branches in lowland oak forests.
Received: August 22, 2002 / Accepted: January 8, 2003
Acknowledgments I am grateful to Dr. Yousuke Degawa (KPM) for allowing the specimens cited to be kept in the Kanagawa Prefectural Museum
of Natural History.
Correspondence to:H. Takahashi 相似文献
12.
Hyaloscypha albohyalina var. monodictys, a new variety in the family Hyaloscyphaceae, Helotiales with Monodictys anamorph is described and illustrated.
Received: June 26, 2002 / Accepted: July 27, 2002
Present address: Strategic Product Portfolio Department, Sankyo Co., Ltd., 3-5-1 Nihonbashi-Honcho, Chuo-ku, Tokyo 103-8426, Japan Tel. +81-3-5255-7040
(Ext. 2528); Fax +81-3-5255-7086 e-mail: hosoya@hq.sankyo.co.jp
Correspondence to:T. Hosoya 相似文献
13.
Non-random seed shadows are commonly seen in plant species whose seeds are dispersed by animals, in particular by birds. The
behaviour of birds can influence the spatial pattern of seed dispersal and, consequently, the entire regeneration process
of fleshy-fruited trees. This study examined regeneration patterns in a fleshy-fruited tree species, rowan (Sorbus aucuparia L.), growing in West Carpathian subalpine spruce forests, focussing on two problems: the temporal relationship between rowan
regeneration and gap formation, and the spatial relationship between rowan regeneration and stand structure. It was found
that rowan seedlings and saplings were recruited in advance of gap formation. Establishment of new rowan individuals in gaps
was infrequent, but gaps enhanced their regeneration nearby under spruce canopy, where they occurred densely in a narrow belt
about 15 m wide. Inside spruce stands, the highest density of young rowans was directly under crowns, especially near trunk
bases. Few rowan saplings were found growing under mature rowan trees. The presence of a rowan seedling and sapling bank determines
whether rowans fill spruce stand gaps. Dense rowan groves can develop mainly in extensive but slowly expanding gaps. 相似文献
14.
Iwaki K Nishida J Yanagisawa T Yoshida H Kato K 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2002,104(4):571-576
To identify a molecular marker closely linked to Vrn-B1, the Vrn-1 ortholog on chromosome 5B, sequence polymorphism at four orthologous RFLP loci closely linked to the Vrn-1 gene family was analyzed by using near-isogenic lines of ”Triple Dirk.” At Xwg644, a RFLP locus, three types of nucleotide sequence differing by the number of (TG) repeats, two or three times, and base changes
were detected. A (TG)3-type sequence proved to be specific to chromosome 5B by nulli-tetrasomic analysis, and substitution of single nucleotide
(C/T) was detected between TD(B) carrying the former Vrn2 allele and TD(C) carrying the vrn2 allele. A mismatch primer was designed for dCAPS analysis of this single nucleotide polymorphism (SNP). Polymorphism was
successfully detected between two NILs, through nested PCR by using a (TG)3-specific primer (1st) and a dCAPS primer (2nd) followed by a NsiI digest. The analysis of a BF2 population [(TD(B)//TD(C)] revealed the close linkage (1.7 cM) between WG644–5B and Vrn2. It was therefore concluded that the former Vrn2 locus is located on chromosome 5B and equivalent to Vrn-B1.
Received: 3 May 2001 / Accepted: 19 July 2001 相似文献
15.
Summary. A novel practical method for the synthesis of N-methyl-DL-aspartic acid 1 (NMA) and new syntheses for N-methyl-aspartic acid derivatives are described. NMA 1, the natural amino acid was synthesized by Michael addition of methylamine to dimethyl fumarate 5. Fumaric or maleic acid mono-ester and -amide were regioselectively transformed into beta-substituted aspartic acid derivatives.
In the cases of maleamic 11a or fumaramic esters 11b, the α-amide derivative 13 was formed, but hydrolysis of the product provided N-methyl-DL-asparagine 9 via base catalyzed ring closure to DL-α-methylamino-succinimide 4, followed by selective ring opening. Efficient methods were developed for the preparation of NMA-α-amide 13 from unprotected NMA via sulphinamide anhydride 15 and aspartic anhydride 3 intermediate products. NMA diamide 16 was prepared from NMA dimethyl ester 6 and methylamino-succinimide 4 by ammonolysis. Temperature-dependent side reactions of methylamino-succinimide 4 led to diazocinone 18, resulted from self-condensation of methylamino-succinimide via nucleophyl ring opening and the subsequent ring-transformation. 相似文献
16.
Summary The generation of transgenic Cucumis sativus cv. Greenlong plants resistant to phosphinothricin (PPT) was obtained using Agrobacterium tumefaciens-mediated gene transfer. The protocol relied on the regeneration of shoots from cotyledon explants. Transformed shoots were
obtained on Murashige and Skoog medium supplemented with 4.4 μM 6-benzylaminopurine 3.8 μM abscisic acid, 108.5 μM adenine sulfate, and 2 mg l−1 phosphinothricin. Cotyledons were inoculated with the strain EHA105 harboring the neomycin phosphotransferase II (npt II), and phosphinothricin resistance (bar) genes conferring resistance to kanamycin and PPT. Transformants were selected by using increasing concentrations of PPT
(2–6 mg l−1). Elongation and rooting of putative transformants were performed on PPT-containing (2 mg l−1) medium with 1.4 μM gibberellic acid and 4.9 μM indolebutyric acid, respectively. Putative transformants were confirmed for transgene insertion through PCR and Southern
analysis. Expression of the bar gene in transformed plants was demonstrated using a leaf painting test with the herbicide Basta. Pre-culture of explants
followed by pricking, addition of 50 μM acetosyringone during infection, and selection using PPT rather than kanamycin were found to enhance transformation frequency
as evidenced by transient β-glucuronidase assay. Out of 431 co-cultivated explants, 7.2% produced shoots that rooted and grew
on PPT, and five different plants (1.1%) were demonstrated to be transgenic following Southern hybridization. 相似文献
17.
The subject of this research is epiphytic yeast communities formed on the surface of Sorbus aucuparia. The object is to make quantitative assessment of the yeast communities’ differentiation of the same but distant substratum. Results of the nested ANOVA demonstrated that with increase in distances, there are increases in the variation of total number and relative abundance of the dominant yeast communities. The average similarity between groups of single fruits (Sørensen’s f Similarity Coefficient) regularly decreased with distance. The results demonstrate that the number and structure of separate yeast groups depend not only on ecological factors but also on proximity to other communities. Such aggregation in the distribution of the microorganisms’ species caused by migration and colonial resettlement should be taken into account when analyzing their diversity in natural habitats. 相似文献
18.
Casa AM Mitchell SE Smith OS Register JC Wessler SR Kresovich S 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2002,104(1):104-110
Recently, a new type of molecular marker has been developed that is based on the presence or absence of the miniature inverted
repeat transposable element (MITE) family Heartbreaker (Hbr) in the maize genome. These so-called Hbr markers have been shown to be stable, highly polymorphic, easily mapped, and evenly distributed throughout the maize genome.
In this work, we used Hbr-derived markers for genetic characterization of a set of maize inbred lines belonging to Stiff Stalk (SS) and Non-Stiff Stalk
(NSS) heterotic groups. In total, 111 markers were evaluated across 62 SS and NSS lines. Seventy six markers (68%) were shared
between the two groups, and 25 of the common markers occurred at fairly low frequency (≤0.20). Only two markers (3%) were
monomorphic in all samples. Although DNA sequencing indicated that 5.5% of same-sized DNA fragments were non-homologous, this
result did not affect the cluster analyses (i.e., relationships obtained from the Hbr data were congruent with those derived from pedigree information). Distance matrices generated from Hbr markers were significantly correlated (p<0.001) with those obtained from pedigree (r=0.782), RFLPs (r=0.747), and SSRs (r=0.719). Overall, these results indicated that Hbr markers could be used in conjunction with other molecular markers for genotyping and relationship studies of related maize
inbred lines.
Received: 26 February 2001 / Accepted: 20 April 2001 相似文献
19.
A marine fungus was isolated from the black tiger prawn Penaeus monodon at Nha Trang, Vietnam, on March 20, 2001 and named isolate NJM 0131. The fungus was identified as Haliphthoros milfordensis from the characteristics of asexual reproduction, and its physiological characteristics were investigated. Although the optimum
temperature for growth of the isolate was 25°–30°C, the fungus grew at a wide range of temperatures (15°–40°C). H. milfordensis grew well in 50%–100% seawater, but poorly in PYG agar containing 1.0%–5.0% NaCl and KCl. The fungus grew at a wide range
of pH (4.0–11.0) with the optimum pH value of 7.0–9.0. The isolate also showed pathogenicity to swimming crab larvae (Portunus trituberculatus) by artificial infection, but mortality was not high. This is the first report of disease in the black tiger prawn P. monodon in Vietnam caused by H. milfordensis.
Received: July 22, 2002 / Accepted: January 21, 2003
Correspondence to:K. Hatai 相似文献
20.
We studied the correlation between pathogenicity and restriction fragment length polymorphism (RFLP) type, which was determined
by polymerase chain reaction-based RFLP analysis of the internal transcribed spacer regions of ribosomal DNA, in the willow
leaf rust fungi Melampsora epitea and M. humilis. Eighteen clones of eight Salix species were inoculated with urediniospores from seven collections of the two rust species. M. epitea and M. humilis (RFLP type-5 collections) were pathogenic to six to eight Salix species. RFLP type-7 collections of M. epitea were pathogenic to only two Salix species. The taxonomic relationships of the two rust species are discussed.
Received: December 11, 2002 / Accepted: February 17, 2003
RID="*"
ID="*" Contribution no. 179, Laboratory of Plant Parasitic Mycology, Institute of Agriculture and Forestry, University of
Tsukuba, Japan
Acknowledgments We thank K. Katsuya, former professor at the University of Tsukuba, for his encouragement in this study. We are also grateful
to M. Yashima, Botanical Garden, University of Tohoku, for his assistance in collecting materials and to R. Suzuki, University
of Tsukuba, for providing a rust isolate. 相似文献