Complexity of species conservation in managed habitats: interaction betweenMaculinea butterflies and their ant hosts

Europe's five species ofMaculinea butterfly are examples of endangered species adapted to live in traditional, cultural landscapes. All are threatened with extinction in Western Europe because of recent changes in land use. This is illustrated by an historical account of the extinction of the BritishMaculinea arion populations, despite many conservation attempts. It is shown how the failures proved to be due to ignorance of the key factor forM. arion, its specialization on a single ant host,Myrmica sabuleti. A brief account is given of research that shows how each of the five species is similarly dependent upon a separate hostMyrmica ant species and how each has an interesting and rare specific parasitoid. Steps for the practical conservation of existingMaculinea populations including the obligation, under the Bern Convention, to re-establish nationally extinct species are outlined. The procedure and problems involved in re-establishment are illustrated with reference to the successful programme forM. arion in Britain. The best way of ensuring robust populations ofMaculinea butterflies is to manage habitats to optimize the density and distribution of the required species ofMyrmica host and, secondarily, the distribution of the larval food plant. The value of single species conservation in cultural habitats is discussed. It is concluded that this is possible to achieve and that other rare organisms also often benefit, but only when conservation measures are based on the results of detailed autecological research.     

Probable evolution of the coelacanth's reproductive style: lecithotrophy and orally feeding embryos in cichlid fishes and in Latimeria chalumnae

Synopsis The living coelacanth is a livebearer. Yolk seems to be the main source of nutrients and of oxygen to the embryo (fetus). Long before birth, young may also possibly feed orally on histotrophe secretion and egg debris. This type of reproduction evolved, as in most other fishes, from oviparity. The Carboniferous coelacanth Rhabdoderma exiguum had eggs of much lesser yolk volume and may represent an earlier form of oviparity with hiding, guarding or brooding type of parental care. The Jurassic coelacanth Holophagus (Undina) and the Cretaceous Axelrodichthys appear to have already evolved the internal-bearing style. Much of this evolutionary sequence is similar to that in cichlids. Ancestral cichlids are substrate tenders and nesters, with small eggs, little yolk and a feeding larva with indirect development. Mouthbrooding cichlids evolved a few, large eggs with denser yolk, direct development and, ultimately, orally feeding embryos while yolk is still in ample supply. Mixed feeding from yolk and orally ingested food in cichlids and in coelacanths is shown to be an enhanced mode of food delivery to the embryos over that from each source separately, in order to produce directly a better developed or larger young at the time of release, i.e. independence. Increase in egg size is regarded as an environmentally induced, altered pattern of yolk synthesis and an initial component of the epigenetic mechanism leading towards greater specialization. Carotenoids are incorporated within the yolk to assist the oxidative metabolism of the developing embryo.     

Trypanosoma rangeli: RAPD-PCR and LSSP-PCR analyses of isolates from southeast Brazil and Colombia and their relation with KPI minicircles

This study presents the first genetic characterization of five Trypanosoma rangeli isolates from Minas Gerais, in the southeast of Brazil and their comparison with Colombian populations by minicircle classification, RAPD-PCR and LSSP-PCR analyses. Our results demonstrated a homogenous T. rangeli population circulating among Didelphis albiventris as reservoir host in Brazil while heterogeneous populations were found in different regions of Colombia. KP1(+) minicircles were found in 100% isolates from Brazil and in 36.4% of the Colombian samples, whereas the KP2 and KP3 minicircles were detected in both groups. RAPD-PCR and LSSP-PCR profiles revealed a polymorphism within KP1(+) and KP1(-) T. rangeli populations and allowed the division of T. rangeli in two branches. The Brazilian KP1(+) isolates were more homogenous than the KP1(+) isolates from Colombia. The RAPD-PCR were entirely consistent with the distribution of KP1 minicircles while those obtained by LSSP-PCR were associated in 88.9% and 71.4% with KP1(+) and KP1(-) populations, respectively.