Lacinutrix jangbogonensis sp. nov., a psychrophilic bacterium isolated from Antarctic marine sediment and emended description of the genus Lacinutrix

A Gram-negative, strictly aerobic, non-motile, rod-shaped and psychrophilic bacterial strain, PAMC 27137^T, was isolated from the marine sediment of the Ross Sea, Antarctica. Strain PAMC 27137^T was observed to grow at 4–10 °C, at pH 6.5–7.5 and in the presence of 2.5–4.0 % (w/v) sea salts. Phylogenetic analysis based on the 16S rRNA gene sequence indicated that strain PAMC 27137^T belongs to the genus Lacinutrix showing the high similarities with Lacinutrix mariniflava JCM 13824^T (97.6 %) and Lacinutrix algicola JCM 13825^T (97.1 %). Genomic relatedness analyses based on the average nucleotide identity and the genome-to-genome distance showed that strain PAMC 27137^T is clearly distinguished from the most closely related Lacinutrix species. The major fatty acids (>5 %) were identified as iso-C_15:1 G (19.9 %), iso-C_15:0 (19.3 %), iso-C_17:0 3-OH (11.3 %), summed feature 9 (C_16:0 10-methyl and/or iso-C_17:1 ω9c as defined by MIDI, 9.1 %), iso-C_15:0 3-OH (7.5 %), and anteiso-C_15:1 A (5.8 %). The polar lipids were found to consist of phosphatidylethanolamine, an unidentified aminolipid, an unidentified aminophospholipid, and five unidentified phospholipids. The major respiratory quinone was identified as MK-6. The genomic DNA G+C content was determined to be 32.1 mol%. Based on the data from this polyphasic taxonomic study, strain PAMC 27137^T is considered to represent a novel species of the genus Lacinutrix, for which the name Lacinutrix jangbogonensis sp. nov. is proposed. The type strain is PAMC 27137^T (=KCTC 32573^T=JCM 19883^T).     

Siansivirga jejunensis sp. nov., isolated from seawater of Jeju Island in Korea and emendation of the genus Siansivirga

A Gram-stain negative, non-flagellated, non-gliding and rod-shaped bacterium, strain JHH-2^T, was isolated from seawater collected in Jeju Island, Korea. The novel isolate was found to grow at 25–30 °C, at pH 6.5–7.0 and in the presence of 1–2 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain JHH-2^T is closely related to Siansivirga zeaxanthinifaciens JCM 17682^T, with 16S rRNA gene sequence similarity of 96.6 %. The predominant respiratory quinone was identified as menaquinone-6 and the major cellular fatty acids were detected as iso-C_15:0, C_16:0 and iso-C_17:0 3-OH. Polar lipid profiling revealed the presence of phosphatidylethanolamine, an unidentified glycolipid, four unidentified aminolipids and two unidentified lipids. The DNA G+C content of the chromosomal DNA of the type strain is 35.6 mol%. On the basis of several distinct phenotypic characteristics and phylogenetic analysis, a new species of the genus Siansivirga, Siansivirga jejunensis JHH-2^T sp. nov. is proposed. The type strain is JHH-2^T (= KCCM 92030^T = JCM 19228^T).     

Ochrovirga pacifica gen. nov., sp. nov., A Novel Agar-Lytic Marine Bacterium of the Family Flavobacteriaceae Isolated From A Seaweed

A strain designated as S85^T was isolated from a seaweed collected from coastal area of Chuuk State in Micronesia. The strain was gram-negative, rod-shaped, and non-motile and formed yellow colonies on the SWY agar (0.2 % yeast extract and 1.5 % agar in seawater) and Marine agar 2216. The strain grew at pH 5–9 (optimum, pH 8), at 15–40 °C (optimum, 25–28 °C), and with 1–9 % (w/v) NaCl (optimum, 3 %). The phylogenetic analysis based on 16S rRNA gene sequence showed that strain S85^T was related to Lutibacter litoralis CL-TF09^T and Maritimimonas rapanae A31^T with 91.4 % and with 90.5 % similarity, respectively. The dominant fatty acids were iso-C_15:0, iso-C_15:0 3-OH and iso-C_17:0 3-OH, C_16:0 3-OH and summed feature 3 (C_16:1 ω7c and/or iso-C_15:0 2-OH). The major isoprenoid quinone was MK-6. The DNA G+C content of the type strain was 34.6 mol %. The major polar lipids were phosphatidylethanolamine, an unknown glycolipid and two unknown polar lipids. Based on this polyphasic taxonomic data, strain S85^T stands for a novel species of a new genus, and we propose the name Ochrovirga pacifica gen. nov., sp. nov. The type strain of O. pacifica is S85^T (=KCCM 90106 =JCM 18327^T).     

Clostridium vulturis sp. nov., isolated from the intestine of the cinereous vulture (Aegypius monachus)

A Gram-stain positive, strict anaerobe, spore-forming, motile rod-shaped bacterial strain with peritrichous flagella, designated YMB-57^T, was isolated from the intestine of a cinereous vulture (Aegypius monachus) in Korea. StrainYMB-57^T was found to show optimal growth at 37 °C, pH 7.5 and 1.0 % (w/v) NaCl. Phylogenetic analysis based on the 16S rRNA gene sequence showed that strain YMB-57^T belongs to the genus Clostridium and is most closely related to the type strains of Clostridium subterminale (96.9 % sequence similarity), Clostridium thiosulfatireducens (96.7 %) and Clostridium sulfidigenes (96.6 %). The main fermentation end-products identified following growth in PYG medium were acetate, butyrate, ethanol, propanol, carbon dioxide and hydrogen. Peptone was converted to ethanol, and butanol, whereas glucose was fermented to ethanol. The major cellular fatty acids were identified as C_16:0, C_18:1 ω9c, and C_18:1 ω9c DMA and the DNA G+C content was determined to be 34.0 mol%. Phenotypic and phylogenetic differences indicate that strain YMB-57^T is distinct from other Clostridium species. It is proposed that strain YMB-57^T be classified as the type strain of a novel species of the genus Clostridium, with the name Clostridium vulturis sp. nov. The type strain is YMB-57^T (=KCTC 15114^T = JCM 17998^T).     

Gordonibacter faecihominis sp. nov., isolated from human faeces

A novel actinobacterial strain, designated CAT-2^T, was isolated from human faeces as a bacterium capable of dehydroxylating (+)-catechin derivatives. Strain CAT-2^T was found to be strictly anaerobic, Gram-positive, non-motile and non-spore-forming coccobacilli. The major fatty acids were identified as C_16:0 DMA (dimethy acetal), C_16:0, C_14:0, anteiso-C_15:0 and iso-C_14:0. The three predominant menaquinones were identified as MK-6 (menaquinene-6), MMK-6 (monomethylmenaquinone-6) and DMMK-6 (dimethylmenaquinone-6). The polar lipids were found to be diphosphatidylglycerol, phosphatidylglycerol and four unidentified glycolipid. The DNA G+C content of strain CAT-2^T was 68.4 mol%. Phylogenetic analysis based on 16S rRNA gene sequence similarities showed that strain CAT-2^T belongs to the genus Gordonibacter, sharing the highest level of sequence homology with Gordonibacter pamelaeae DSM 19378^T (97.3 %). Combined phenotypic, chemotaxonomic and phylogenetic characteristics support the conclusion that the strain CAT-2^T represents a novel species, for which the name Gordonibacter faecihominis sp. nov. is proposed. The type strain is CAT-2^T (= KCTC 15204^T = JCM 16058^T).     

Aquimarina penaei sp. nov., isolated from intestinal tract contents of Pacific white shrimp,Penaeus vannamei

A novel bacterial strain designated P3-1^T was isolated from the intestinal tract contents of Pacific white shrimp (Penaeus vannamei) in Zhangpu, Fujian province, China. The isolate was found to be Gram-negative, long rod shaped, oxidase- and catalase- positive. Growth was observed at 1–7 % sea salt (w/v, optimum, 3 %), at pH 7.0–9.0 (optimum, pH 7.0) and at 10–37 °C (optimum, 28 °C). The isolate was capable of hydrolysing gelatin, casein, starch and DNA but unable to degrade Tween 20, 40, 80 and cellulose. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain P3-1^T belongs to the genus Aquimarina, with highest sequence similarity to Aquimarina gracilis PSC32^T (96.2 %), followed by Aquimarina intermedia KMM 6258^T (96.1 %), Aquimarina spongiae A6^T (95.9 %) and Aquimarina muelleri KMM 6020^T (95.8 %). The principal cellular fatty acids were identified as iso-C_15:0, iso-C_17:0 3OH, C_16:1 ω7c/ω6c, iso-C_15:1 G, iso-C_15:0 3OH, iso-C_17:1 ω9c/C_16:0 10-methyl and C_16:0. The G+C content of the chromosomal DNA was determined to be 33.3 mol%. The respiratory quinone was determined to be MK-6 (100 %). The combined genotypic and phenotypic data show that strain P3-1^T represents a novel species within the genus Aquimarina, for which the name Aquimarina penaei sp. nov. is proposed, with the type strain P3-1^T (=MCCC 1A09871^T = LMG 27943^T).     

Bizionia psychrotolerans sp. nov., a psychrophilic bacterium isolated from the intestine of a sea cucumber (Apostichopus japonicus)

A novel Gram-negative, non-flagellated, non-gliding, rod-shaped bacterial strain, designated PB-M7^T, was isolated from the intestine of a sea cucumber collected from Pohang, South Korea. Growth was observed at 4–30 °C (optimum, 25 °C), pH 6.0–9.0 (optimum, pH 7.0–8.0), and with 2.0–6.0 % (w/v) NaCl (optimum, 2.0 %). In a phylogenetic analysis based on 16S rRNA gene sequences, strain PB-M7^T was found to belong to the genus Bizionia and to be most closely related to Bizionia echini KMM 6177^T (99.0 % 16S rRNA gene sequence similarity), Bizionia hallyeonensis T-y7^T (97.9 %), Bizionia algoritergicola APA-1^T (97.5 %), Bizionia argentinensis JUB59^T (97.5 %) and Bizionia myxarmorum ADA-4^T (97.1 %). The predominant fatty acids of strain PB-M7^T were identified as iso-C_15:0 (22.2 %), iso-C_15:1 G (10.8 %), iso-C_17:0 3-OH (16.7 %) and summed feature 3 (C_16:1 ω7c and/or C_16:1 ω6c; 11.2 %). The major respiratory quinone was identified as menaquinone-6 (MK-6). The polar lipid profile of strain PB-M7^T was found to contain phosphatidylethanolamine, an unidentified phospholipid, three unidentified aminolipids and three unidentified lipids. The DNA G + C content of strain PB-M7^T was determined to be 33.4 mol% and the mean DNA–DNA relatedness values with the type strains of B. echini, B. hallyeonensis, B. algoritergicola, B. argentinensis, and B. myxarmorum were 52.9, 48.5, 46.5, 37.1 and 26.6 %, respectively. Based on the data presented, strain PB-M7^T represents a novel species of the genus Bizionia, for which the name Bizionia psychrotolerans sp. nov. is proposed. The type strain of B. psychrotolerans is PB-M7^T (= KCCM 43042 ^T = JCM 19924 ^T).     

Bacillus haikouensis sp. nov., a facultatively anaerobic halotolerant bacterium isolated from a paddy soil

A Gram-stain positive, rod-shaped, endospore-forming and facultatively anaerobic halotolerant bacterium, designated as C-89^T, was isolated from a paddy field soil in Haikou, Hainan Province, People’s Republic of China. Optimal growth was observed at 37 °C and pH 7.0 in the presence of 4 % NaCl (w/v). The predominant menaquinone was identified as MK-7, the major cellular fatty acids were identified as anteiso-C_15:0 and iso-C_15:0, and the major cellular polar lipids were identified as phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol and two unknown phospholipids. The peptidoglycan type was determined to be based on meso-DAP. Based on 16S rRNA gene sequence similarity, the closest phylogenetic relatives were identified as Bacillus vietnamensis JCM 11124^T (98.8 % sequence similarity), Bacillus aquimaris JCM 11545^T (98.6 %) and Bacillus marisflavi JCM 11544^T (98.5 %). The DNA G+C content of strain C-89^T was determined to be 45.4 mol%. The DNA–DNA relatedness values of strain C-89^T with its closest relatives were below 18 %. Therefore, on the basis of phylogenetic, chemotaxonomic, and phenotypic results, strain C-89^T can be considered to represent a novel species within the genus Bacillus, for which the name Bacillus haikouensis sp. nov., is proposed. The type strain is C-89^T (=KCTC 33545^T = CCTCC AB 2014076^T).     

Flavobacterium arsenitoxidans sp. nov., an arsenite-oxidizing bacterium from Thai soil

An arsenite-oxidizing bacterium, strain S2-3H^T, was isolated from arsenic-contaminated soil sample collected from Dantchaeng district, Suphanburi province, Thailand and was characterized based on polyphasic taxonomic study. The strain was observed to be a Gram-stain negative, aerobic, yellow pigmented, non-spore forming and rod-shaped bacterium. Major menaquinone was MK-6. Iso-C_15:0, iso-C_15:0 3OH, C_16:1 ω7c/C_16:1 ω6c, C_16:0, iso-C_17:0 3OH, and C_16:0 3OH were the predominant cellular fatty acids. The polar lipid profile consisted of phosphatidylethanolamine, unidentified phospholipids and unidentified aminophospholipids. The DNA G+C content was 37.0 mol%. Phylogenetic analysis using 16S rRNA sequence showed that strain S2-3H^T is affiliated to the genus Flavobacterium, and is closely related to F. defluvii KCTC 12612^T (97.0 %) and F. johnsoniae NBRC 14942^T (97.0 %). The strain S2-3H^T could be clearly distinguished from the related Flavobacterium species by its physiological and biochemical characteristics as well as its phylogenetic position and DNA–DNA relatedness. Therefore, the strain represents a novel species of the genus Flavobacterium, for which the name Flavobacterium arsenitoxidans sp. nov. (type strain S2-3H^T = KCTC 22507^T = NBRC 109607^T = PCU 331^T = TISTR 2238^T) is proposed.     

Flavobacterium ahnfeltiae sp. nov., a new marine polysaccharide-degrading bacterium isolated from a Pacific red alga

A Gram-negative, aerobic, rod-shaped, motile by gliding and yellow-pigmented bacterium, designated strain 10Alg 130^T, that displayed the ability to destroy polysaccharides of red and brown algae, was isolated from the red alga Ahnfeltia tobuchiensis. The phylogenetic analysis based on 16S rRNA gene sequence placed the novel strain within the genus Flavobacterium, the type genus of the family Flavobacteriaceae, the phylum Bacteroidetes, with sequence similarities of 96.2 and 95.7 % to Flavobacterium jumunjiense KCTC 23618^T and Flavobacterium ponti CCUG 58402^T, and 95.3–92.5 % to other recognized Flavobacterium species. The prevalent fatty acids of strain 10Alg 130^T were iso-C_15:0, iso-C_15:0 3-OH, iso-C_17:0 3-OH, C_15:0 and iso-C_17:1ω9c. The polar lipid profile consisted of phosphatidylethanolamine, two unknown aminolipids and three unknown lipids. The DNA G+C content of the type strain was 34.3 mol%. The new isolate and the type strains of recognized species of the genus Flavobacterium could strongly be distinguished by a number of phenotypic characteristics. A combination of the genotypic and phenotypic data showed that the algal isolate represents a novel species of the genus Flavobacterium, for which the name Flavobacterium ahnfeltiae sp. nov. is proposed. The type strain is 10Alg 130^T (=KCTC 32467^T = KMM 6686^T).     

Oceanobacillus halophilum sp. nov. Isolated from a Mangrove Forest Soil

A halophilic, aerobic bacterium, designated GD01^T, was isolated from a mangrove forest soil near the South China Sea. Cells of strain GD01^T were Gram staining positive, oxidase positive, and catalase positive. The strain was rod shaped and motile by means of peritrichous flagella and produced ellipsoidal endospores. The strain was able to grow with NaCl at concentrations of 0.5–12 % (optimum 3–5 %, w/v), at temperatures of 20–50 °C (optimum 30 °C), and at pH 6.0–8.5 (optimum pH 7.0). Phylogenetic analysis based on 16S rRNA gene sequences showed that strain GD01^T formed a cluster with O. profundus DSM 18246^T (96.4 % 16S rRNA gene sequence similarity), O. caeni KCTC 13061^T (95.4 %), and O. oncorhynchi JCM 12661^T (94.5 %). The G+C content of strain GD01^T was 38.7 mol%. The major respiratory quinone was MK-7. The major cellular fatty acids (>5 %) were anteiso-C_15:0, iso-C_16:0 (13.7 %), anteiso-C_17:0 (12.6 %), iso-C_15:0 (9.9 %), iso-C_14:0 (9.5 %), and C_16:0 (5.0 %). The polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol, glycolipid, four unknown lipids, and four unknown phospholipids. Based on phenotypic characteristics, chemotaxonomic features, and phylogenetic analysis based on 16S rRNA gene sequences, the strain was identified to represent a distinct novel species in the genus Oceanobacillus, and the name proposed is Oceanobacillus halophilum sp. nov. with type train GD01^T (=CCTCC AB 2012863^T = KCTC 33101^T).     

Sphingobacterium pakistanensis sp. nov., a novel plant growth promoting rhizobacteria isolated from rhizosphere of Vigna mungo

The taxonomic status of a bacterium, strain NCCP-246^T, isolated from rhizosphere of Vigna mungo, was determined using a polyphasic taxonomic approach. The strain NCCP-246^T can grow at 16–37 °C (optimum 32 °C), at pH ranges of 6–8 (optimum growth occurs at pH 7) and in 0–4 % (w/v) NaCl. Phylogenetic analysis based upon on 16S rRNA gene sequence comparison revealed that strain NCCP-246^T belonged to genus Sphingobacterium. Strain NCCP-246^T showed highest similarity to the type strain of Sphingobacterium canadense CR11^T (97.67 %) and less than 97 % with other species of the genus. The DNA–DNA relatedness value of strain NCCP-246^T with S. canadense CR11^T and Sphingobacterium thalpophilum JCM 21153^T was 55 and 44.4 %, respectively. The chemotaxonomic data revealed the major menaquinone as MK-7 and dominant cellular fatty acids were summed feature 3 [C_16:1 ω7c/C_16:1 ω6c] (37.07 %), iso-C_15:0 (28.03 %), C_16:0 (11.85 %), C_17:0 cyclo (8.84 %) and C_14:0 (2.42 %). The G+C content of the strain was 39.2 mol%. On the basis of DNA–DNA hybridization, phylogenetic analyses, physiological and, biochemical data, strain NCCP-246^T can be differentiated from the validly named members of genus Sphingobacterium and thus represents as a new species, for which the name, Sphingobacterium pakistanensis sp. nov. is proposed with the type strain NCCP-246^T (= JCM18974 ^T = KCTC 23914^T).     

Polaribacter marinivivus sp. nov., a member of the family Flavobacteriaceae isolated from seawater

A Gram-stain negative, non-flagellated, non-gliding, aerobic and rod-shaped bacterium, designated GYSW-15^T, was isolated from a seawater of Geoje island on the South Sea, South Korea, and subjected to a polyphasic taxonomic approach. Strain GYSW-15^T was found to grow optimally at 30 °C, at pH 7.0–8.0 and in the presence of approximately 2.0 % (w/v) NaCl. Neighbour-joining phylogenetic tree based on 16S rRNA gene sequences showed that strain GYSW-15^T belongs to the genus Polaribacter, clustering coherently with the type strain of Polaribacter porphyrae showing sequence similarity value of 97.0 %. Strain GYSW-15^T exhibited 16S rRNA gene sequence similarity values of 94.3–96.4 % to the type strains of the other Polaribacter species. Strain GYSW-15^T was found to contain MK-6 as the predominant menaquinone and iso-C_15:0, iso-C_15:1 G, iso-C_16:0 3-OH and summed feature 3 (C_16:1 ω7c and/or C_16:1 ω6c) as the major fatty acids. The polar lipid profile of strain GYSW-15^T containing phosphatidylethanolamine and an unidentified lipid as major components was similar to that of the type strain of P. porphyrae. The DNA G + C content of strain GYSW-15^T was determined to be 31.2 mol% and its mean DNA–DNA relatedness with P. porphyrae LMG 26671^T was 17 %. Differential phenotypic properties, together with its phylogenetic and genetic distinctiveness, revealed that strain GYSW-15^T is separated from recognized Polaribacter species. On the basis of the data presented, strain GYSW-15^T is considered to represent a novel species of the genus Polaribacter, for which the name Polaribacter marinivivus sp. nov. is proposed. The type strain is GYSW-15^T ( = KCTC 42156^T = CECT 8655^T)     

Oceanobacillus aidingensis sp. nov., a moderately halophilic bacterium

Two Gram-positive, rod-shaped moderately halophilic bacterial strains, designated AD7-25^T and AB-11, were isolated from Aiding and Manasi salt lakes in Xinjiang of China, respectively. The strains were found to be able to grow at NaCl concentrations of 0–21 % (w/v), with optimum growth occurring at 6–8 % (w/v) NaCl. The optimal temperature and pH for growth were determined to be 33–37 °C and pH 7.0–7.5. Cells of the strains are motile by means of polar flagella. Both strains can produce ellipsoidal spores. The major cellular fatty acids were identified as anteiso-C_15:0, iso-C_15:0, iso-C_14:0, anteiso-C_17:0 and iso-C_16:0. The diamino acid in the peptidoglycan and the major quinone system were determined to be meso-diaminopimelic acid (meso-DAP) and MK-7, respectively. The DNA G+C contents of stains AD7-25^T and AB-11 were 39.8 and 40.0 mol%, respectively. Phylogenetic analysis based on 16S rRNA gene sequences revealed that these two novel strains are closely related to the genus Oceanobacillus showing 90–99.5 % similarity with respect to type strains. These two novel strains were most closely related to Oceanobacillus oncorhynchi subsp. incaldanensis DSM 16557^T (99.1 and 99.5 %), followed by O. oncorhynchi subsp. oncorhynchi JCM 12661^T (99.1 and 99.4 %), Oceanobacillus neutriphilus CGMCC 1.7693^T (97.0 and 97.5 %), Oceanobacillus sojae JCM 15792^T (97.6 and 98.0 %) and Oceanobacillus locisalsi KCTC 13253^T (96.5 and 96.9 %). The DNA–DNA hybridization data indicated that DNA relatedness between strains AD7-25^T and AB-11 was 91.0 %, and the genomic homology of representative strain AD7-25^T with O. oncorhynchi subsp. incaldanensis DSM 16557^T, O. oncorhynchi subsp. oncorhynchi JCM 12661^T, O. neutriphilus CGMCC 1.7693^T, O. sojae JCM 15792^T and O. locisalsi KCTC 13253^T were 41, 39, 20, 23 and 17 %, respectively. On the basis of phenotypic and phylogenetic distinctiveness, strains AD7-25^T and AB-11 should be assigned to the genus Oceanobacillus as a new species, for which the name Oceanobacillus aidingensis sp. nov. was proposed. The type strain is AD7-25^T (=CGMCC 1.9106 ^T = NBRC 105904^T).     

Saccharomonospora oceani sp. nov. isolated from marine sediments in Little Andaman, India

Two actinomycete strains, designated YIM M11168^T and YIM M11177, were isolated from marine sediment samples from Little Andaman, Indian Ocean, and their taxonomic position was determined by a polyphasic approach. The two Gram-positive, aerobic strains were observed to produce branched substrate mycelium and aerial hyphae but did not fragment, and no diffusible pigment was produced on the media tested. At maturity, spores were formed singly or in pairs on aerial hyphae and substrate mycelium, and occasionally the single ones were borne on long sporophores. The optimum growth was determined to occur at 28 °C, 0–4 % (w/v) NaCl and pH 7.0–8.0. Whole-cell hydrolysates of both strains contained meso-diaminopimelic acid and the diagnostic sugars were determined to be galactose, glucose and arabinose. Their predominant menaquinone was found to be MK-9(H₄). The polar lipids detected in the two strains were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol mannoside, phosphatidylinositol, phosphatidylmethylethanolamine, phosphatidylethanolamine and two unknown phosphoglycolipids. The major fatty acids (>10 %) identified were iso-C_16:0, iso-C_16:1 H, iso-C_16:0, C_17:1 ω6c for strain YIM M11168^T, iso-C_16:0 and Summed Feature 3 for strain YIM M11177. The G + C contents of the genomic DNAs of both strains were determined to be 71.4 %. DNA–DNA hybridization relatedness values (78.4 ± 3.7 %) of these two isolates supported the conclusion that they belong to the same species. Based on phylogenetic analysis, phenotypic and genotypic data, it is concluded that the two isolates belong to a novel species of the genus Saccharomonospora of the family Pseudonocardiaceae. The name Saccharomonospora oceani sp. nov. (Type strain YIM M11168^T = DSM 45700^T = JCM 18128^T) is proposed for the novel species.     

Streptomyces canchipurensis sp. nov., isolated from a limestone habitat

Hundung Limestone habitat, Manipur, India is an unexplored site for microbial diversity studies. Using polyphasic taxonomy, a Streptomyces strain, MBRL 172^T, has been characterized. The strain was found to show highest 16S rRNA gene sequence similarity with Streptomyces coeruleofuscus NBRC 12757^T (99.2 %). The DNA relatedness between MBRL 172^T and S. coeruleofuscus NBRC 12757^T, and between MBRL 172^T and Streptomyces nogalater NBRC 13445^T, were 36.8 ± 4.4 and 52.5 ± 2.7 %, respectively. Strain MBRL 172^T was found to contain ll-diaminopimelic acid as the diagnostic diamino acid and glucose, mannose and xylose as the major sugars in whole cell hydrolysates. The polar lipids in the cell membrane were identified as diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol and phosphatidylinositolmannoside. The predominant menaquinones detected were MK-9(H₆) and MK-9(H₈). The cellular fatty acids identified were mainly saturated fatty acids: anteiso-C_15:0, iso-C_16:0 and iso-C_15:0. Based on differences in the biochemical and molecular characteristics from its closest relatives, the strain can be proposed to represent a novel taxon in the genus Streptomyces, for which the name Streptomyces canchipurensis is proposed, with the type strain MBRL 172^T (=JCM 17575^T = KCTC 29105^T).     

Hymenobacter qilianensis sp. nov., isolated from a subsurface sandstone sediment in the permafrost region of Qilian Mountains,China and emended description of the genus Hymenobacter

A red–pink, Gram-negative, rod-shaped, non-motile, non-spore-forming bacterium, designated strain DK6-37 was isolated from the permafrost region of Qilian Mountains in northwest of China. Phylogenetic analysis based on 16S rRNA gene sequencing indicated that this isolate represents a novel member of the genus Hymenobacter, with low sequence similarities (<97 %) to recognized Hymenobacter species. Optimum growth was observed at 28 °C, pH 7.0 and 0 % NaCl. The strain was found to contain MK-7 as the predominant menaquinone. The polar lipids were identified as phosphatidylethanolanmine, two unknown aminophospholipids, one unknown aminolipid and three unknown polar lipids. The major fatty acids were identified as summed feature 3 (C_16:1 ω7c/C_16:1 ω6c as defined by MIDI), summed feature 4 (anteiso-C_17:1 B/iso-C_17:1 I), C_16:1 ω5c, iso-C_17:0 3-OH, iso-C_15:0 and C_18:0. The DNA G + C content was determined to be 67.4 mol %. On the basis of the polyphasic evidence presented, it is proposed that strain DK6-37 represents a novel species of the genus Hymenobacter, for which the name Hymenobacter qilianensis sp. nov. is proposed. The type strain is DK6-37^T (= CGMCC 1.12720^T = JCM 19763^T).     

Aliiglaciecola coringensis sp. nov., isolated from a water sample collected from mangrove forest in Coringa,Andhra Pradesh,India

A Gram-negative, rod shaped, motile, aerobic bacterium, designated as strain AK49^T was isolated from a water sample from a mangrove forest in Coringa village, Andhra Pradesh, India. Strain AK49^T was observed to form yellow coloured, smooth, circular, convex colonies on marine agar, with entire margins. Cells of strain AK49^T are 0.5–1.0 µm wide and 1.5–3.5 µm long. Growth was observed at 25–37 °C (optimum 30 °C), 2–6 % NaCl (optimum 2 %) and pH 6–8 (optimum 7). Phylogenetic analysis based on 16S rRNA gene sequences showed that the strain AK49^T is closely related to two species recently reclassified as members of the genus Aliiglaciecola: Aliiglaciecola lipolytica JCM 15139^T (sequence similarity 95.43 %) and Aliiglaciecola litoralis JCM 15896^T (sequence similarity 96.91 %). The major cellular fatty acids of strain AK49^T were found to include C_16:0, C_18:1ω7c and summed feature 3 (C_16:1ω7c/C_15:0 iso-2-OH). The polar lipid content of cell membrane was found to include phosphatidylethanolamine, phosphatidylglycerol, an unidentified aminolipid, an unidentified lipid and an unidentified glycolipid. The genomic DNA G+C content of strain AK49^T was determined to be 41.9 mol%. Based on the taxonomic methods, including chemotaxonomic, phenotypic and phylogenetic approaches, strain AK49^T is described here as a novel species belonging to the genus Aliiglaciecola, for which the name Aliiglaciecola coringensis sp. nov. is proposed. The type strain of Aliiglaciecola coringensis sp. nov. is AK49^T (=MTCC 12003^T = JCM19197^T).