Host plant resistance among tomato accessions to the spider mite <Emphasis Type="Italic">Tetranychus evansi</Emphasis> in Kenya

The spider mite Tetranychus evansi has a broad range of host plants. Control of T. evansi has been a big challenge to tomato farmers due to its fast rate of reproduction, development of resistance to chemical pesticides and its ability to use weeds as alternative hosts when the tomato plants are not available. The aim of the current study was to determine the host plant acceptance and the relative contributions of trichomes in the control of the red spider mite by comparing the survival, development and oviposition rates of the red spider mite on eight tomato accessions. Leaflets from eight tomato varieties were assayed with the spider mites to determine the egg laying capacity and developmental time of the spider mites on the tomato accessions as well as the trichome densities. Densities of trichome types I, IV, V and VI varied among the tomato accessions. Variation in types I, IV and VI accounted for most of the variation in mite responses. The varieties with high densities of types IV and VI had the highest fecundity and mite development did not go beyond the larval stage. The developmental time varied significantly among the tomato accessions. The results indicated that the higher the density of trichome type I the lower the adult survival. The findings indicated possible resistance of some of the tested tomato accessions against T. evansi which is partially associated with trichomes types and density.     

Population density and phenology of Tetranychus urticae (Acari: Tetranychidae) in hop is linked to the timing of sulfur applications

The twospotted spider mite, Tetranychus urticae Koch, is a worldwide pest of numerous agronomic and horticultural plants. Sulfur fungicides are known to induce outbreaks of this pest on several crops, although mechanisms associated with sulfur-induced mite outbreaks are largely unknown. Studies were conducted during 2007-2009 in Oregon and Washington hop yards to evaluate the effect of timing of sulfur applications on T. urticae and key predators. In both regions, applications of sulfur made relatively late in the growing season (mid-June to mid-July) were associated with the greatest exacerbation of spider mite outbreaks, particularly in the upper canopy of the crop. The severity of mite outbreaks was closely associated with sulfur applications made during a relatively narrow time period coincident with the early exponential phase of spider mite increase and rapid host growth. A nonlinear model relating mean cumulative mite days during the time of sulfur sprays to the percent increase in total cumulative mite days (standardized to a nontreated plot) explained 58% of the variability observed in increased spider mite severity related to sulfur spray timing. Spatial patterns of spider mites in the Oregon plots indicated similar dispersal of motile stages of spider mites among leaves treated with sulfur versus nontreated leaves; however, in two of three years, eggs were less aggregated on leaves of sulfur-treated plants, pointing to enhanced dispersal. Apart from one experiment in Washington, relatively few predatory mites were observed during the course of these studies, and sulfur-induced mite outbreaks generally occurred irrespective of predatory mite abundance. Collectively, these studies indicate sulfur induces mite outbreaks through direct or indirect effects on T. urticae, mostly independent of predatory mite abundance or toxicity to these predators. Avoidance of exacerbation of spider mite outbreaks by sulfur sprays was achieved by carefully timing applications to periods of low spider mite abundance and slower host development, which is generally early to mid-spring for hop.     

Strategic and tactical modeling: cotton-spider mite agroecosystem management

We discuss the development of two simulation models, a mechanistic cotton crop model and a spider mite population model. In a strategic mode, the simulation models are used to address basic hypotheses involving the interaction of cotton with its environment, and with the spider mite population, and to develop general strategies for managing the crop and its herbivores. In a tactical mode, these models are used to provide estimates of the anticipated severity of a spider mite population. Tactical modelling is made possible by using a statistically-based adaptive interface. The departure of simulated patterns of growth from observed patterns is used to adapt several crop parameters including rate of photosynthesis, rate of vegetative growth, and metabolite allocation priority for fruit. For the spider mite population model, fecundity, predator-mediated mortality, and acaricide-induced mortality are adapted to specific field conditions.     

Consequences of mite feeding injury to beans on the fecundity and survivorship of the two-spotted spider mite (Acari: Tetranychidae)

Fecundity and survival of the two-spotted spider mite,Tetranychus urticae Koch, were examined on bean (Phaseolus vulgaris L.) plants that had been subjected to mite feeding injury in the laboratory. Different numbers ofT. urticae were restricted on the first two leaves of young bean plants, and spider-mite fecundity and survivorship was assayed on the third leaf. Each plant received four recently enclosed females, one female from each of four mite lineages. Using changes in the ratio of root mass to shoot mass of bean plants as a continuous measure of plant stress from spider-mite feeding, fecundity was positively related to stress for three out of four experiments. In two out of four experiments, survival of females was also positively related to stress, but reached an asymptote at slight or moderate stress levels. No evidence for induced resistance in beans was found. Mite lineage and the interaction between lineage and stress affected female survival but not fecundity. The implications of these results for understanding spider-mite outbreaks are discussed.     

Life history traits of Aphis gossypii Glover (Hom., Aphididae) reared on four widely distributed weeds

Variation in the life history traits of cotton aphids, Aphis gossypii Glover, reared on four widely distributed weeds, Ageratum houstonianum Mill., Bidens pilosa L. var. radiata Sch. Bip., Solanum nigrum L. and Spermacoce latifolia Aubl., were investigated. Cotton aphids were reared in the laboratory at 25°C. Each host plant had a distinct effect on aphid life history traits. Cotton aphids reared on S. nigrum had a significantly shorter developmental period, and age-specific fecundity peaked early. In contrast, cotton aphids reared on S. latifolia had a long developmental period and low age-specific fecundity. Cotton aphids that fed on B. pilosa and A. houstonianum displayed intermediate rates of growth and age-specific fecundity. Because the curves of age-specific fecundity ( m_x ) and age-specific net maternity ( l_x  m _x ) on each host plant were close together, development time and the pattern of age-specific fecundity were the major factors determining the population growth potential of the cotton aphid on each weed. As a result, the intrinsic rate of population growth for aphids reared on S. nigrum was significantly higher ( r_m =0.527 ± 0.011) than it was for aphids reared on S. latifolia ( r_m =0.194 ± 0.012).     

Effects of temperature on two Mediterranean population of Dinophilus gyrociliatus (Polychaeta: Dinophilidae): II. Effects on demographic parameters

The effects of temperature on demographic characteristics of two populations from Ravenna and Genoa of the polychaete Dinophilus gyrociliatus were investigated. Temperature affects age-specific survival and fecundity and all the demographic parameters often to a different degree in the two populations. Individuals from Ravenna survive longer than those from Genoa. The most evident differences in the age-specific fecundity curves of the experimental groups are related to age at maturity and the duration of the reproductive period that are in inverse proportion to temperature. In both populations of D. gyrociliatus, the maximum daily fecundity is observed at intermediate temperatures. In all cases, the Genoa females mature earlier, attain their maximum fecundity more quickly and have a shorter reproductive period than their Ravenna counterparts.Age at maturity, fecundity during the first reproductive events and juvenile survival are by far the most important characteristics in determining the fitness of the two populations at the tested temperatures. Even though the greatest net growth rates and highest expectation of life were recorded at 12 °C in the Ravenna population, the delay in the attainment of sexual maturity means that, at this temperature, the population growth rate is lowest. The higher juvenile survivorship and the greater fecundity observed at 24 °C is counter-balanced by the early attainment of sexual maturity induced at 30 °C. The comparison of the population growth rate calculated in laboratory with field data suggests that temperature is one of the main environmental parameters determining the fitness of D. gyrociliatus.     

不同三叶草对二斑叶螨生长发育和繁殖的影响

【目的】明确红三叶、白三叶和杂三叶3种寄主对二斑叶螨Tetranychus urticae Koch生长发育和繁殖的影响,以及3种寄主间二斑叶螨的生物学差异。【方法】在室内(25±1)℃条件下,采用离体叶片法研究了3种三叶草对二斑叶螨种群参数的影响。【结果】3种三叶草对二斑叶螨生长发育、雌成螨寿命以及繁殖力有显著影响(P<0.05)。在杂三叶上,二斑叶螨发育历期、产卵前期明显比红三叶和白三叶上要长,而且雌成螨寿命和产卵期也明显缩短。每雌产卵量在红三叶上最高(150.87粒),白三叶次之(139.43粒),而杂三叶上最低(86.95粒)。二斑叶螨在3种三叶草上的存活曲线均为Ⅰ型,存活率高低依次为红三叶、白三叶和杂三叶。净增殖率(R0)、内禀增长率(rm)和周限增长率(λ)在红三叶上最高、杂三叶上最低,而平均世代周期(T)和种群加倍时间(Dt)在杂三叶上最长、红三叶上最短。【结论】3种三叶草对二斑叶螨的适合度具有一定差异,红三叶和白三叶对二斑叶螨具有更高的适合度。     

The host plant-mediated impact of simulated acid rain on the development and reproduction of Tetranychus cinnabarinus (Acari, Tetranychidae)

Abstract:  The host plant-mediated impact of simulated acid rain (pH 3.0, 3.5, 4.0, 5.6, and 6.8) on the behaviour, development, and reproduction of carmine spider mite, Tetranychus cinnabarinus (Boisduvals), were evaluated at 25°C in a series of laboratory trials. The results indicated that the adults of carmine spider mite prefer to aggregate on pH 4.0 acid rain-treated eggplant leaves. The developmental times of nymph, larva and the pre-oviposition period of the mite on kidney bean leaves were significantly affected by acid rain treatments. The developmental times for the immature stages ranged from 11.4 days on pH 4.0 acid rain-treated leaves to 12.7 days on other acid rain-treated leaves. The survival of immatures varied from 85.14% on pH 4.0 acid rain-treated leaves to 78.83% on deionized water-treated leaves (pH 6.8). The mites feeding on acid rain-treated leaves (pH 3.0–5.6) had significantly greater reproductivity and longevity than those feeding on deionized water-treated leaves. The average greatest reproductivity (74.6 eggs per female) and longest female longevity (8.9 days) were recorded on pH 4.0 acid rain-treated leaves. Accordingly, the intrinsic rate of increase ( r _m ) for the mite on pH 4.0 acid rain-treated leaves was the highest. Jackknife estimates of r _m varied from 0.201 on pH 4.0 acid rain-treated leaves to 0.158 on pH 5.6 acid rain-treated leaves. The results implied that the population growth of the mite were enhanced by application of acid rain (pH 3.0–5.6) on host plants.     

温度对扶桑绵粉蚧生长发育的影响

【目的】扶桑绵粉蚧Phenacoccus solenopsis Tinsley是近年在我国新发现的一种重要外来入侵害虫,对我国棉花生产具有潜在的巨大威胁。本研究以棉花作为寄主,探索恒温及变温条件对扶桑绵粉蚧生长发育的影响。【方法】在光周期为12L∶12D,RH 70%±5％,在系列恒温及变温（温度波动范围为±1℃）条件下,用棉花饲养扶桑绵粉蚧,观察和分析了不同温度下各龄虫态的发育历期、发育速率、存活率和繁殖力及发育起点温度、有效积温、最适发育温度、极限高温等。【结果】在恒温17~32℃范围内,扶桑绵粉蚧各虫态的发育历期随温度升高而逐渐缩短,当温度达到27℃时发育速率值增幅最大,其中在恒温22~32℃范围内,该虫具有较高的生长发育速率和存活率,且繁殖能力较强,而在恒温37℃条件下,该虫不能完成整个生活史而死亡。在循环变温条件（25~40℃）下,该虫表现出更好的适应性：发育历期更短,速率更快,存活率更高,繁殖力也达到了恒温条件下的平均水平。雌雄虫的发育起点温度分别为9.0℃和8.1℃,而达到成虫时所需有效积温分别为322.6日度和344.8日度。通过拟合发育速率与温度之间的非线性回归关系求出雌雄虫最适发育温度分别为30.5℃和29.9℃,而极限高温分别为36.6℃和35.8℃。【结论】扶桑绵粉蚧适温范围广泛,特别是在变温条件下的适应性非常强。这些结果为预测扶桑绵粉蚧在我国主要棉区的分布提供了依据。     

Reproduction and survival of the predatory bugOrius albidipennis on various arthropod prey

The daily rate of oviposition, fecundity, survival and adult longevity ofOrius albidipennis (Reuter) (Hemiptera: Anthocoridae) were studied in the laboratory in Israel. These parameters were compared on three arthropod prey species: the two-spotted spider mite,Tetranychus urticae Koch; the onion thrips,Thrips tabaci Lindeman; and eggs of the almond moth,Ephestia cautella Walker. The fecundity and survival on the thrips diet (217.2 eggs/female and 98.7%, respectively) and on moth eggs (184.1 eggs/female and 84.6%, respectively) were significantly higher than on the spider mite diet (110.9 eggs/female and 40.4%, respectively). Female longevity was significantly higher onEphestia eggs (63.0 days) than on thrips (45.1 days) and mites (35.1 days). There were no significant differences in male longevity among the three diets (57.5, 64.1 and 54.5 days, respectively).     

Relationship between dispersal and fecundity ofTetranychus urticae Koch (Acari: Tetranychidae) exposed to flucythrinate

The effects of the synthetic pyrethroid flucythrinate on dispersal and fecundity of the two-spotted spider mite,Tetranychus urticae Koch, was investigated using a vial exposure technique that allowed treatment of mites without incidental treatment of host plant and allowed determination of the actual dose received by the mites. Flycythrinate elicited both immediate and delayed dispersal. Immediate dispersal within 120 min posttreatment was more sensitive than delayed dispersal and fecundity at 24, 48, and 72 h posttreatment. The lowest flucythrinate concentrations elicited immediate dispersal without subsequent inhibition of fecundity, providing support for the hypothesis that pyrethroid-induced mite population outbreaks can result from dispersal of healthy mites to areas of low competition. Delayed dispersal, however, was observed only in mites that had reduced fecundity. No significant increases in egg production were observed; however, mites exposed to the lowest concentrations of flucythrinate showed a trend toward increased fecundity at 48 and 72 h posttreatment. Since the mites were treated rather than the host plant, it appeared that the dispersal associated with flucythrinate was manifested primarily through a general stimulation of locomotor activity rather than directional locomotor response.Contribution from the Missouri Agricultural Experiment Station, Columbia, MO, Journal Series No. 10037.     

温度和氮肥对褐飞虱存活、生长发育和繁殖的交互作用

在实验室条件下,研究了不同温度（20 ℃、23 ℃、26 ℃、29 ℃和32 ℃）和氮肥水平（不施氮：0 kg·hm^-2;高氮：250 kg·hm^-2）对褐飞虱存活、发育和繁殖的影响和交互作用.结果表明：在20 ℃~29 ℃范围内,随着温度的升高,褐飞虱的卵孵化率和若虫存活率提高,卵和若虫历期缩短,成虫寿命缩短,同时产卵量增加;32 ℃时, 褐飞虱卵孵化率和若虫存活率降低、卵和若虫历期延长、成虫生殖力降低;在不同温度下,高氮植株上的褐飞虱卵孵化率和若虫存活率、成虫生殖力均明显高于低氮稻株上的褐飞虱,同时高氮植株上褐飞虱的卵和若虫历期明显短于低氮稻株上的褐飞虱,说明施用高水平氮肥提高了褐飞虱对逆境条件的生态适应性;温度和氮肥对褐飞虱卵存活率、若虫历期和产卵量的交互作用显著,表明全球气候变暖和长期施用高水平氮肥很可能是近年来褐飞虱猖獗暴发的重要原因.     

Overwintering survival and postdiapause fecundity in a population of the Kanzawa spider mite <Emphasis Type="Italic">Tetranychus kanzawai</Emphasis> (Acari: Tetranychidae) on <Emphasis Type="Italic">Orixa japonica</Emphasis> (Rutaceae)

In several adult-diapausing insects and mites, long duration of diapause decreases egg production after diapause termination. However, such nonlethal effects are much less studied than overwintering survival. In this study, lethal and nonlethal effects of different periods of diapause were investigated in an adult-diapausing spider mite, Tetranychus kanzawai Kishida (Acari: Tetranychidae), through field experiments. Diapausing females produced in the laboratory were kept in their natural habitat from October or November to the next April, and survival rate and postdiapause fecundity were compared between the months. The survival rate was lower, but not significantly, in the October treatment. Postdiapause fecundity was also not significantly different between the months. These results suggest that the effects of diapause on survival and reproduction are quite small after October, when diapausing mites are increasing in their natural habitat.     

Demographic analyses of organophosphate-resistant and susceptible strains of greenhouse whitefly, Trialeurodes vaporariorum, on three cotton cultivars

Laboratory experiments were conducted using organophosphate-resistant and susceptible strains of greenhouse whitefly, Trialeurodes vaporariorum Westwood, to assess age-specific vital rates in individually-held adults, and development and survival in preadults on three cotton cultivars at 27±1 °C, 50±10% RH, and a photoperiod of 16:8 (L:D). Female whiteflies lived longer than males, with a maximum life expectancy of 29 days. Heaviest egg laying occurred at ages between 7 and 18 days when individual whiteflies laid > 10 eggs/day. Greenhouse whitefly populations doubled weekly, with stable age distribution of 63% eggs, 28% larvae, 5% pupae, and 4% adults. Analysis of various life history parameters that combine aspects of survival, developmental rates, and fecundity indicated no consistent differences in reproductive fitness between the two greenhouse whitefly strains. Of the three cotton cultivars tested, Pima S-6 was most susceptible, Acala SJ-2 was intermediate and Gumbo 500 was most resistant to greenhouse whitefly. Resistance of Gumbo 500 was expressed as slower developmental rates, reduced survival to adulthood, lower reproductive rates, and lower intrinsic rate of increase.     

The interaction of two-spotted spider mites, <Emphasis Type="Italic">Tetranychus urticae</Emphasis> Koch,with Cry protein production and predation by <Emphasis Type="Italic">Amblyseius andersoni</Emphasis> (Chant) in Cry1Ac/Cry2Ab cotton and Cry1F maize

Crops producing insecticidal crystal (Cry) proteins from the bacterium, Bacillus thuringiensis (Bt), are an important tool for managing lepidopteran pests on cotton and maize. However, the effects of these Bt crops on non-target organisms, especially natural enemies that provide biological control services, are required to be addressed in an environmental risk assessment. Amblyseius andersoni (Acari: Phytoseiidae) is a cosmopolitan predator of the two-spotted spider mite, Tetranychus urticae (Acari: Tetranychidae), a significant pest of cotton and maize. Tri-trophic studies were conducted to assess the potential effects of Cry1Ac/Cry2Ab cotton and Cry1F maize on life history parameters (survival rate, development time, fecundity and egg hatching rate) of A. andersoni. We confirmed that these Bt crops have no effects on the biology of T. urticae and, in turn, that there were no differences in any of the life history parameters of A. andersoni when it fed on T. urticae feeding on Cry1Ac/Cry2Ab or non-Bt cotton and Cry1F or non-Bt maize. Use of a susceptible insect assay demonstrated that T. urticae contained biologically active Cry proteins. Cry proteins concentrations declined greatly as they moved from plants to herbivores to predators and protein concentration did not appear to be related to mite density. Free-choice experiments revealed that A. andersoni had no preference for Cry1Ac/Cry2Ab cotton or Cry1F maize-reared T. urticae compared with those reared on non-Bt cotton or maize. Collectively these results provide strong evidence that these crops can complement other integrated pest management tactics including biological control.     

Photoperiodic induction of diapause in the spider mite Tetranychus urticae: qualitative or quantitative time measurement?

Abstract. Insects and mites may measure photoperiods eitfier by classifying them as long or short relative to a critical value (qualitative time measurement) or by using the absolute value (quantitative time measurement). The spider mite Tetranychus urticae is thought to use a qualitative mechanism of time measurement. In this paper we present the results of experiments with an inbred line of the spider mite (to keep genetic variation in photoperiodic responses small), to test whether quantitative aspects also play a role. Differences in diapause incidence in different long-night photoperiods at different temperatures may be an indication of quantitative responses to photoperiod. The effect of temperature on the photoperiodic response curve was studied at 16^oC, 19^oC and 22^oC. The response curves appeared to be similar at 16^oC and 19^oC, with a critical nightlength between 10 and 11 h. At 22^oC, diapause induction was less than 100% in all long-night regimens and die critical nightlength had shifted to 12 h. Maximum diapause induction (93%) occurred in a light-dark cycle with a 16 h dark phase (LD 8:16 h). Diapause induction was lowest in long-night photoperiods with dark phases of 20 h and longer. The number of light-dark cycles needed for 50% diapause induction at 19^oC varied. between 12.1 and 14.7 for LD 6:18 h, between 10.9 and 12.5 for LD 8:16 h, between 10.6 and 11.6 for LD 10:14 h, and between 10.1 and 10.7 for LD 12:12 h. Independent of die light-dark regimen, diapause induction took place in some individuals after receiving 8 cycles and virtually all individuals entered diapause after 16 cycles. No effect was found of the photoperiodic treatment during prediapause development (LD 6:18 h, LD 8:16 h, LD 10:14 h, LD 12:12 h) on diapause duration. The average diapause duration at LD 10:14 h and 19^oC was 61 days over all four treatments. We explained the results by hypothesising that nightlengths are assessed qualitatively and mat the photoperiodic clock operates more accurately near the critical nightlength.     

基于生命表参数的棉铃虫种群动态模拟研究

依据对棉铃虫自然种群生命表研究所获得的种群统计参数,采用蒙特卡罗方法,模拟了随机环境条件下的棉铃虫种群发生动态。模拟结果显示,同一输入参数(模拟的1代残虫量),经过确定世代或生活史阶段后,棉铃虫的种群密度既可能为轻发生,也可能种群暴发。环境条件有利时,输入变量(模拟的1代残虫量)影响棉铃虫最大可能的发生程度。当棉铃虫为中等或轻发生时,1代残虫量(模型输入参数)和最终的棉铃虫发生程度(模型输出)没有明显的依赖关系。相同的最终棉铃虫发生程度所对应的模型的输入变量可以有多种水平。模拟结果显示,在环境条件比较有利情况下,棉铃虫只需要1个世代或某一个生活史阶段(如蛹期),种群密度即可达暴发水平。本文的研究结果说明,对棉铃虫中长期发生态势的预测,存在一定程度的不确定性。     

冷驯化对异色瓢虫后代生长发育及适合度的影响

为明确冷驯化处理对异色瓢虫Harmonia axyridis后代生长发育及适合度的影响, 本研究通过两性生命表的制作, 在室内条件下调查了冷处理后其后代发育历期、 成虫产卵前期、 寿命和生殖力以及后代生命表参数。结果表明: 冷驯化使异色瓢虫后代卵的发育历期延长, 幼虫（1-4龄）和蛹的发育历期则缩短; 随着冷驯化时间的延长, 后代体长和体重增量均减小。且完成发育的后代成虫产卵前期延长, 寿命缩短, 生殖力下降。后代生命表参数内禀增长率（r）、 周限增长率（λ）、 净生殖率（R₀）和年龄特征存活率（l_x）均降低, 但是后代雌虫所占比例却升高。此外, 冷驯化类型对异色瓢虫后代生长发育的影响也不相同。经相同时间（5 d）的低温诱导, 变温诱导的后代成虫寿命比恒定低温诱导的长, 但是生殖力却低; 变温诱导的后代生命表参数（r, λ和R₀）均小于恒定低温诱导的, 但是l_x却高于恒定低温诱导的。结果说明, 异色瓢虫亲代经历冷驯化, 这种对低温的可塑性反应会延伸到下一代, 而且还能够影响后代的适合度, 这对其在低温下的存活和繁殖具有重要的意义。     

Effects of temperature and food quality on life-history parameters in Parameletus chelifer and P. minor (Ephemeroptera): a laboratory study

SUMMARY. 1. Growth rate of Parameletus minor was greatest between 10.8 and 19.8°C, survival rate peaked at 5.9°C, developmental time was shortest at 14.6°C, and adult size and fecundity reached maximum values between 5.9 and 10.8°C. Growth rate of P. chelifer was greatest between 14.6 and 19.8°C, survival rate peaked at 5.9°C, and developmental time was shortest at 14.6°C. A large adult size was found at 10.8°C, and highest fecundity between 10.8 and 14.6°C.
2. Food quality significantly affected growth rate, developmental time, adult size and fecundity of both species. Both P. chelifer and P minor attained highest growth rate, largest adult size and highest fecundity when the C/N ratio of food was 5.95. Developmental time was shortest at a C/N ratio between 5.95 and 12.8.
3. Nymphs of P. chelifer had a higher temperature 'optimum' for growth than nymphs of P. minor . Growth rate of nymphs of P. chelifer reared on detritus from a seasonal stream (C/N ratio 12.8) was about 3 times that of nymphs reared on detritus from a river margin (C/N ratio 20.9). The corresponding growth rate difference for nymphs of P. minor was only about 1.5.
4. When all life-history parameters are taken into consideration, P. chelifer had a higher temperature 'optimum' than P. minor .     

Jasmonic acid induced resistance in grapevines to a root and leaf feeder

We investigated the effects of induced resistance to the folivore Pacific spider mite, Tetranychus pacificus McGregor (Acari: Tetranychidae), as well as the root-feeding grape phylloxera Daktulosphaira vitifoliae (Fitch) (Homoptera: Phylloxeridae) in grapevines using exogenous applications of the natural plant inducer, jasmonic acid. Foliar jasmonic acid application at concentrations that caused no phytotoxicity significantly reduced the performance of both herbivores. There were less than half as many eggs produced by spider mites feeding on the induced leaves compared with control grapevine leaves. Induction reduced the numbers of phylloxera eggs and nymphal instars by approximately threefold and twofold, respectively, on induced compared with control grapevine roots. The negative demographic effects of jasmonic acid application appeared to be caused by changes in fecundity for the Pacific spider mite, and possibly changes in development rate and fecundity for grape phylloxera.