共查询到20条相似文献,搜索用时 31 毫秒
1.
Su-Lian Lv Li-Jun Lian Pei-Lin Tao Zhao-Xia Li Ke-Wei Zhang Ju-Ren Zhang 《Planta》2009,229(4):899-910
An H+-PPase gene, TsVP from Thellungiella halophila, was transferred into two cotton (Gossypium hirsutum) varieties (Lumianyan19 and Lumianyan 21) and southern and northern blotting analysis showed the foreign gene was integrated
into the cotton genome and expressed. The measurement of isolated vacuolar membrane vesicles demonstrated that the transgenic
plants had higher V–H+-PPase activity compared with wild-type plants (WT). Overexpressing TsVP in cotton improved shoot and root growth, and transgenic plants were much more resistant to osmotic/drought stress than the
WT. Under drought stress conditions, transgenic plants had higher chlorophyll content, improved photosynthesis, higher relative
water content of leaves and less cell membrane damage than WT. We ascribe these properties to improved root development and
the lower solute potential resulting from higher solute content such as soluble sugars and free amino acids in the transgenic
plants. In this study, the average seed cotton yields of transgenic plants from Lumianyan 19 and Lumianyan 21 were significantly
increased compared with those of WT after exposing to drought stress for 21 days at flowering stage. The average seed cotton
yields were 51 and 40% higher than in their WT counterparts, respectively. This study benefits efforts to improve cotton yields
in arid and semiarid regions. 相似文献
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To investigate the relationship between nuclear factor Y (NF-Y) and stress tolerance in garlic, we cloned a NF-Y family gene AsNF-YC8 from garlic, which was largely upregulated at dehydrate stage. Expression pattern analyses in garlic revealed that AsNF-YC8 is induced through abscisic acid (ABA) and abiotic stresses, such as NaCl and PEG. Compared with wild-type plants, the overexpressing-AsNF-YC8 transgenic tobacco plants showed higher seed germination rates, longer root length and better plant growth under salt and drought stresses. Under drought stress, the transgenic plants maintained higher relative water content (RWC), net photosynthesis, lower levels of malondialdehyde (MDA), and less ion leakage (IL) than wild-type control plants. These results indicate the high tolerance of the transgenic plants to drought stress compared to the WT. The transgenic tobacco lines accumulated less reactive oxygen species (ROS) and exhibited higher antioxidative enzyme activities compared with wild-type (WT) plants under drought stress, which suggested that the overexpression of AsNF-YC8 improves the antioxidant defense system by regulating the activities of these antioxidant enzymes, which in turn protect transgenic lines against drought stress. These results suggest that AsNF-YC8 plays an important role in tolerance to drought and salt stresses. 相似文献
3.
Enhanced expression of phospholipase C 1 (ZmPLC1) improves drought tolerance in transgenic maize 总被引:2,自引:0,他引:2
Phosphatidylinositol-specific phospholipase C (PI-PLC) plays an important role in a variety of physiological processes in
plants, including drought tolerance. It has been reported that the ZmPLC1 gene cloned from maize (Zea mays L.) encoded a PI-PLC and up-regulated the expression in maize roots under dehydration conditions (Zhai SM, Sui ZH, Yang AF,
Zhang JR in Biotechnol Lett 27:799–804, 2005). In this paper, transgenic maize expressing ZmPLC1 transgenes in sense or antisense orientation were generated by Agrobacterium-mediated transformation and confirmed by Southern blot analysis. High-level expression of the transgene was confirmed by
real-time RT-PCR and PI-PLC activity assay. The tolerance to drought stress (DS) of the homogenous transgenic maize plants
was investigated at two developmental stages. The results demonstrated that, under DS conditions, the sense transgenic plants
had higher relative water content, better osmotic adjustment, increased photosynthesis rates, lower percentage of ion leakage
and less lipid membrane peroxidation, higher grain yield than the WT; whereas those expressing the antisense transgene exhibited
inferior characters compared with the WT. It was concluded that enhanced expression of sense ZmPLC1 improved the drought tolerance of maize. 相似文献
4.
Guiping Diao Yucheng Wang Chao Wang Chuanping Yang 《Plant Molecular Biology Reporter》2011,29(1):77-87
Plant glutathione S-transferases (GSTs) are involved in protecting plants against both diverse biotic and abiotic stresses. In the present study,
a novel GST gene (LbGST1) was cloned from Limonium bicolor (Bunge) Kuntze (Plumbaginaceae). To characterize its function in salt tolerance, tobacco lines transformed with LbGST1 were generated. Compared with wild-type (WT) tobacco, transgenic plants overexpressing LbGST1 exhibited both GST and glutathione peroxidase activities. Moreover, superoxide dismutase, peroxidase (POD), and catalase
activities in transgenic plants were significantly higher than those in WT plants, particularly when grown under conditions
of salt stress. Similarly, levels of proline in transgenic plants were also higher than those in WT plants grown under NaCl
stress conditions. Whereas, Malondialdehyde contents in transgenic plants were lower than those in WT plants under NaCl conditions.
Furthermore, Na+ content in transgenic plants was lower than that in WT plants under these stress conditions. Subcellular localization analysis
revealed that the LbGST1 protein was localized in the nucleus. These results suggested that overexpression of LbGST1 gene can affect many physiological processes associated with plant salt tolerance. Therefore, we hypothesize that LbGST1 gene can mediate many physiological pathways that enhance stress resistance in plants. 相似文献
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Hongxia Liu Yunpeng Wang Xiaosi Zhou Cui Wang Chao Wang Jia Fu Tian Wei 《Plant cell reports》2016,35(6):1333-1344
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Chenxi Xu Lei Zheng Caiqiu Gao Chao Wang Guifeng Liu Jing Jiang Yucheng Wang 《Plant Molecular Biology Reporter》2011,29(2):424-430
H+-ATPase subunit c (VHA-c) is involved in the adaptation to environmental stresses, including salt, drought, and heavy metals.
However, it remains unclear whether VHA-c can induce a physiological response related to stress tolerance. To investigate
this possibility, we generated transgenic tobacco lines overexpressing a V-ATPase subunit c (LbVHA-c1) gene from Limonium bicolor (Bunge) Kuntze. Compared with wild-type (WT) tobacco, superoxide dismutase (SOD) and peroxidase (POD) activities in the transgenic
plants were significantly enhanced under salt stress conditions. The level of malondialdehyde (MDA) in the transgenic plants
was significantly lower than that in WT plants grown under salt stress conditions. Moreover, the transgenic plants displayed
obviously better growth than the WT plants under salt stress. These results suggest that LbVHA-c1 may confer stress tolerance through enhancing POD and SOD activities, and by protecting membranes from damage by decreasing
lipid peroxidation under salt stress. 相似文献
9.
Liang Liu Ying Wang Nan Wang Yuan‐Yuan Dong Xiu‐Duo Fan Xiu‐Ming Liu Jing Yang Hai‐Yan Li 《植物学报(英文版)》2011,53(9):731-742
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Xiaohui Yu Qiong Gao Guoping Chen Jun-E Guo Xuhu Guo Boyan Tang Zongli Hu 《Plant Molecular Biology Reporter》2018,36(1):36-44
Histone deacetylation catalyzed by histone deacetylases is an important type of histone modification. Histone deacetylases affect various processes of plant development and involve in responding to hormones and biotic and abiotic stresses. Here, we report a tomato PRD3/HDA1 histone deacetylase gene, SlHDA5, which is expressed ubiquitously in different tissues and development stages. Expression profiles in hormone treatments showed that SlHDA5 was induced by abscisic acid (ABA) and methyl jasmonate (MeJA). Seedlings growth of SlHDA5-RNAi lines were more inhibited on the medium containing salt compared with wild type (WT). Under salt stress, chlorophyll in mature leaves degraded earlier in transgenic leaves than that in WT, and transgenic plants displayed wilting earlier and more severe than WT. After drought treatment, transgenic plants wilted and dehydrated earlier than WT, which was confirmed by lower water and chlorophyll content, and higher malondialdehyde (MDA) content in transgenic plants manifesting that the tolerance of transgenic plants to drought receded. Under the treatment of ABA, root length of transgenic seedlings was more strongly repressed by contrast with WT, suggesting repression of SlHDA5 increased seedling sensibility to ABA. Our study indicated that silencing of SlHDA5 resulted in decreasing tolerance to salt, drought, and ABA. 相似文献
13.
Yu Cheng Wang Guan Zheng Qu Hong Yan Li Ying Jie Wu Chao Wang Gui Feng Liu Chuan Ping Yang 《Molecular biology reports》2010,37(2):1119-1124
Superoxide dismutases (SODs) play important role in stress tolerance of plants. In this study, an MnSOD gene (TaMnSOD) from Tamarix androssowii, under the control of the CaMV35S promoter, was introduced into poplar (Populus davidiana × P. bolleana). The physiological parameters, including SOD activity, malondialdehyde (MDA) content, relative electrical conductivity (REC)
and relative weight gain, of transgenic lines and wild type (WT) plants, were measured and compared. The results showed that
SOD activity was enhanced in transgenic plants, and the MDA content and REC were significantly decreased compared to WT plants
when exposed to NaCl stress. In addition, the relative weight gains of the transgenic plants were 8- to 23-fold of those observed
for WT plants after NaCl stress for 30 days. The data showed that the SOD activities that increased in transgenic lines are
1.3–4-folds of that increased in the WT plant when exposed to NaCl stress. Our analysis showed that increases in SOD activities
as low as 0.15-fold can also significantly enhance salt tolerance in transgenic plants, suggesting an important role of increased
SOD activity in plant salt tolerance. 相似文献
14.
Ariadna Peremarti Ludovic Bassie Paul Christou Teresa Capell 《Plant molecular biology》2009,70(3):253-264
Polyamines are known to play important roles in plant stress tolerance but it has been difficult to determine precise functions
for each type of polyamine and their interrelationships. To dissect the roles of putrescine from the higher polyamines spermidine
and spermine, we generated transgenic rice plants constitutively expressing a heterologous S-adenosylmethionine decarboxylase (SAMDC) gene from Datura stramonium so that spermidine and spermine levels could be investigated while maintaining a constant putrescine pool. Whereas transgenic
plants expressing arginine decarboxylase (ADC) produced higher levels of putrescine, spermidine and spermine, and were protected from drought stress, transgenic plants
expressing SAMDC produced normal levels of putrescine and showed drought symptoms typical of wild type plants under stress,
but the transgenic plants showed a much more robust recovery on return to normal conditions (90% full recovery compared to
25% partial recovery for wild type plants). At the molecular level, both wild type and transgenic plants showed transient
reductions in the levels of endogenous ADC1 and SAMDC mRNA, but only wild type plants showed a spike in putrescine levels under stress. In transgenic plants, there was no spike
in putrescine but a smooth increase in spermine levels at the expense of spermidine. These results confirm and extend the
threshold model for polyamine activity in drought stress, and attribute individual roles to putrescine, spermidine and spermine. 相似文献
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Yongan Zhang Qinghua Wang Xue Zhang Xingliang Liu Ping Wang Yuxia Hou 《Journal of Plant Biology》2011,54(5):303-313
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Asif MA Zafar Y Iqbal J Iqbal MM Rashid U Ali GM Arif A Nazir F 《Molecular biotechnology》2011,49(3):250-256
Salinity and drought are main threat to agriculture productivity, to avoid further losses it is necessary to improve the genetic
material of crops against these stresses In this present study, AtNHX1, a vacuolar type Na+/H+ antiporter gene driven by 35S promoter was introduced into groundnut using Agrobacterium tumefaciens transformation system. The stable integration of the AtNHX1 gene was confirmed by polymerase chain reaction (PCR) and southern blot analysis. It was found that transgenic plants having
AtNHX1 gene are more resistant to high concentration of salt and water deprivation than the wild type plants. Salt and proline level
in the leaves of the transgenic plants were also much higher than that of wild type plants. The results showed that overexpression
of AtNHX1 gene not only improved salt tolerance but also drought tolerance in transgenic groundnut. Our results suggest that these
plants could be cultivated in salt and drought-affected soils. 相似文献
19.
Late embryogenesis abundant (LEA) proteins are closely related to abiotic stress tolerance of plants. In the present study, we identified a novel Em-like gene from lettuce, termed LsEm1, which could be classified into group 1 LEA proteins, and shared high homology with Cynara cardunculus Em protein. The LsEm1 protein contained three different 20-mer conserved elements (C-element, N-element, and M-element) in the C-termini, N-termini, and middle-region, respectively. The LsEm1 mRNAs were accumulated in all examined tissues during the flowering and mature stages, with a little accumulation in the roots and leaves during the seedling stage. Furthermore, the LsEm1 gene was also expressed in response to salt, dehydration, abscisic acid (ABA), and cold stresses in young seedlings. The LsEm1 protein could effectively reduce damage to the lactate dehydrogenase (LDH) and protect LDH activity under desiccation and salt treatments. The Escherichia coli cells overexpressing the LsEm1 gene showed a growth advantage over the control under drought and salt stresses. Moreover, LsEm1-overexpressing rice seeds were relatively sensitive to exogenously applied ABA, suggesting that the LsEm1 gene might depend on an ABA signaling pathway in response to environmental stresses. The transgenic rice plants overexpressing the LsEm1 gene showed higher tolerance to drought and salt stresses than did wild-type (WT) plants on the basis of the germination performances, higher survival rates, higher chlorophyll content, more accumulation of soluble sugar, lower relative electrolyte leakage, and higher superoxide dismutase activity under stress conditions. The LsEm1-overexpressing rice lines also showed less yield loss compared with WT rice under stress conditions. Furthermore, the LsEm1 gene had a positive effect on the expression of the OsCDPK9, OsCDPK13, OsCDPK15, OsCDPK25, and rab21 (rab16a) genes in transgenic rice under drought and salt stress conditions, implying that overexpression of these genes may be involved in the enhanced drought and salt tolerance of transgenic rice. Thus, this work paves the way for improvement in tolerance of crops by genetic engineering breeding. 相似文献
20.
Sulian Lv Aifang Yang Kewei Zhang Lei Wang Juren Zhang 《Molecular breeding : new strategies in plant improvement》2007,20(3):233-248
The tolerance to drought stress of the homozygous transgenic cotton (Gossypium hirsutum L.) plants with enhanced glycinebetaine (GB) accumulation was investigated at three development stages. Among the five transgenic
lines investigated, lines 1, 3, 4, and 5 accumulated significantly higher levels of GB than the wild-type (WT) plants either
before or after drought stress, and the transgenic plants were more tolerant to drought stress than the wild-type counterparts
from young seedlings to flowering plants. Under drought stress conditions, transgenic lines 1, 3, 4, and 5 had higher relative
water content, increased photosynthesis, better osmotic adjustment (OA), a lower percentage of ion leakage, and less lipid
membrane peroxidation than WT plants. The GB levels in transgenic plants were positively correlated with drought tolerance
under water stress. The results suggested that GB may not only protect the integrity of the cell membrane from drought stress
damage, but also be involved in OA in transgenic cotton plants. Most importantly, the seedcotton yield of transgenic line
4 was significantly greater than that of WT plants after drought stress, which is of great value in cotton production. 相似文献