西文新刊介绍

1.《Biomedical and Environmental Science》(美国)1988年创刊,馆藏:1989年v.2开始,索刊号。58/B60.6,ISSN 0895—3988 2.《Cytotechnology》(荷兰)1987年创刊,馆藏,1989年v.2开始,索刊号,58/C99,ISSN 0920—9069 3.《Free Radical Research Communications》(美国)1985年创刊,馆藏,1989年v.5开始,索刊号:58/F53,ISSN 8755—0199 4.《Second messengers and Phosphoproteins》(美国)1975年创刊,馆藏:1988年v.12开始,索刊     

刊物变名

1.Cell and Tissue Kinetics (英国), 1968年创刊→Cell Proliferation. 馆藏:v.24-1991-索刊号:58/C34.5 2.Journal of Biological Response Modifiers (美国), 1982年创刊→Journal of Immunotherapy. 馆藏:v.10-1991-索刊号:58/J61.5 3.Bioprocessing Technology (美国),1979年创刊→Industrial Bioprocessing.馆藏:v.13:6-1991-索刊号:58/I50.5     

西文期刊介绍

1.Biodegradation (荷兰)1990年创刊,馆藏: V.1-1990-索刊号:58/B28.5 2.Biosensers & Bioelectronics (英国)1990年创刊,馆藏:V.6-1991-索刊号:58/B63.801 3.Bioseparation (荷兰)1990年创刊,馆藏:V.1-1990-索刊号:58/B63.803 4.Critical Reviews in Eukaryotic Gene Expression (美国)1990年创刊,馆藏:     

刊物变名

1.CRC critical reviews in analytical。h。mistry(美国),1970年创刊,索刊号:54/Clo~Critical reviews in analytical ehemistry馆藏:V.21一1989一索刊号:SJ/C84 .55 2.CRC critical reviews in miCrobiology(美国),1971年创刊,索刊号:58/C15一CritiCal revi。ws in oierobiology馆藏:V.17一1959一索刊号:58/C62 3.Ex,。eri:n。ntal eell biolog了(瑞士),1978年创刊,索+jJ号:55.7/E66~Pathobiology馆藏:V.58一1990一索刊号:58.7/Pso.2 4.Gene analysis technique(美国),1954年创刊,索刊号:58/G23.5~Genetie ana-lysis馆藏:V.7…     

西文新刊介绍

1.Cytokine(美国)1989年创刊,馆藏,v.3-1991-索刊号:58/C92 该刊主要发表细胞激素所有高质量原始论文,例如内白细胞素、细胞毒素、造血因子、转化生成因子、干扰素以及其它或新的细胞激素。包括基础或应用研究,涉及种系发生、分子遗传学、基因表达、生化、病理、诊断或临床治疗应用。 2.Human Antibodies and Hybridomas(美国)1990年创刊,馆藏:v.2-1991-索     

新刊介绍

1.《Journal of Biochemical Toxicology》(生化毒理学杂志)(美国)1988年创刊,索刊号58.7/J53.5 2.《Brain, Behavior and Immunity》(细胞、行为和免疫)(美国)1988年创刊,索刊号61/B36.1 3.《Ag Biotechnology News》(农业生物技术新闻)(美国)1987年创刊,索刊号65/A29     

Increasing drug resistance in human lung cancer cells by mutant-type p53 gene mediated by retrovirus

Human mutant-type (mt) p53 cDNA was synthesized and cloned from human lung cancer cell line GL containing mt-p53 gene by using polymerase chain reaction (PCR). It was confirmed that the mt-p53 cDNA con-tained the complete coding sequence of p53 gene but mutated at codon 245 (G→T) and resulted in glycine to cysteine by sequencing analysis. The retroviral vector pD53M of the mt-p53 was constructed and introduced into the drug-sen-sitive human lung cancer cells GAO in which p53 gene did not mutate. The transfected GAO cells strongly expressed mutant-type p53 protein by immunohistochemistry, showing that pD53M vector could steadily express in GAO cells. The drug resistance to several anticancer agents of GAO cells infected by pD53M increased in varying degrees, with the highest increase of 4-fold, in vitro and in vivo. By quantitative PCR and flow cytometry (FCM) analyses, the expression of MDR1 gene and the activity of P-glycoprotein (Pgp) did not increase, the expression of MRP gene and the activity of m     

Downregulation of wild-type p53 protein by HER-2／neu mediated PI3K pathway activation in human breast cancer cells： its effect on cell proliferation and implication for therapy

Overexpression and activation of HER-2/neu (also known as c-erbB-2), a proto-oncogene, was found in about 30% of human breast cancers, promoting cancer growth and making cancer cells resistant to chemo- and radio-therapy.Wild-type p53 is crucial in regulating cell growth and apoptosis and is found to be mutated or deleted in 60-70% of human cancers. And some cancers with a wild-type p53 do not have normal p53 function, suggesting that it is implicated in a complex process regulated by many factors. In the present study, we showed that the overexpression of HER-2/neu could decrease the amount of wild-type p53 protein via activating PI3K pathway, as well as inducing MDM2 nuclear translocation in MCF7 human breast cancer cells. Blockage of PI3K pathway with its specific inhibitor LY294002 caused G1-S phase arrest, decreased cell growth rate and increased chemo- and radio-therapeutic sensitivity in MCF7 cells expressing wild-type p53. However, it did not increase the sensitivity to adriamycin in MDA-MB-453 breast cancer cells containing mutant p53. Our study indicates that blocking PI3K pathway activation mediated by HER-2/neu overexpression may be useful in the treatment of breast tumors with HER-2/neu overexpression and wild-type p53.     

Growth factor receptor trafficking as a potential therapeutic target in pediatric cancer

Growth factor receptors (GFRs) are often aberrantly expressed in tumor cells,and altered GFR expression and activity contribute to the pathogenesis of many types of cancer.A variety of mechanisms have ...     

中国科学院图书馆馆藏生物工程及其有关外文专书(英文)

Dahlem Workshop on Biotechnology: Potentials and Limitations (1985. Mar.: Berlin)Biotechnology: potentials and limitations: report of the…/S. Silver, editor.--Berlin: Springer-Verlag, c1986. 312 p. (58. 1734083/D131/1985)     

Pro-apoptotic BAX and BAK mediate p53-independent suppression of tumorigenesis

BAX and BAK are essential regulators of apoptotic signaling through mitochondria in mammalian development and in response to cytotoxic stimuli. To investigate the role of BAX and BAK in transformation and tumorigenesis, primary baby mouse kidney epithelial cells (BMKs) from wild-type, BAX, BAK and BAK and BAK deficient mice were transformed by adenovirus E1A and dominant-negative p53 (p53DD). While E1A alone transforms p53 deficient BMKs, transformation of BAX and/or BAK deficient BMKs still required inactivation of p53. Since BAX and BAK are dispensable for p53 to suppress transformation, but     

Cells in G2／M phase increased in human nasopharyngeal carcinoma cellline by EBV-LMP1 through activation of NF-kB and AP-1

Although previous studies showed that the principal oncoprotein encoded by Epstein-Barr virus, latent membrane protein 1 (LMP1), could induce the nasopharyngeal carcinoma cells in G2/M phase increased, littleis known about the target molecules and mechanisms. The present study demonstrated that LMP1 couldinduce the accumulation of p53 protein and upregulate its transactivity in a dose dependent manner, whichresulted in the decrease of the kinase activity of cdc2/cyclin B complex and inducing arrest at G2/M phasethrough the activation of NF-kB and AP-1 signaling pathways, and the effect of NF-kB was more obviousthan that of AP-1. This study provided some significant evidence for further elucidating the molecular mechanisms that LMP1 had effects on the surveillance mechanism of cell cycle and promoting the survivalof transformed cells and tumorigenesis.     

Human infection with a further evolved avian H9N2 influenza A virus in Sichuan,China

正Dear Editor,Avian influenza A (H9N2) virus plays a crucial role in interspecies transmission between animals and humans due to its wide host range, adaptation in mammals and the diversified gene reassortments. Since 1990s, two lineages of H9N2 virus, A/Duck/HK/Y280/97 (Y280-like)and A/Quail/HK/G1/97 (G1) have established in domestic poultry in China and are now widespread in the whole     

Human TRMT1 catalyzes m2G or (m2)2G formation on tRNAs in a substrate-dependent manner

TRMT1 is an N²-methylguanosine(m²G) and N²,N²-methylguanosine((m²)₂G) methyltransferase that targets G26 of both cytoplasmic and mitochondrial t RNAs. In higher eukaryotes, most cytoplasmic t RNAs with G26 carry (m²)₂G26, although the majority of mitochondrial G26-containing t RNAs carry m²G26 or G26, suggesting differences in the mechanisms by which TRMT1 catalyzes modification of these t RNAs. Lo...     

Genetic aberration in primary hepatocellular carcinoma：correlation between p53 gene mutation and loss—of—heterozygosity on chromosome 16q21—q23 and 9p21—p23

To elucidate the molecular pathology underlying the development of hepatocellular carcinoma (HCC),we used 41 highly polymorphic microsatellite markers to examine 55 HCC and corresponding non-tumor liver tissues on chromosome 9,16 and 17.Loss-of-heterozygosity(LOH) is observed with high frequency on chromosomal region 17p13(36k/55,65%),9q21-p23(28/55,51%),16q21-23(27/55,49%) in tumors.Meanwhile,microsatellite instability is rarely found in these microsatellite loci.Direct sequencing was performed to detect the tentative mutation of tumor wuppressor genes in these regions:p53,MTS1/p16,and CDH1/E-cadherin.Wihin exon 5-9 of p53 gene,14 out of 55 HCC specimens(24%) have somatic mutations,and nucleotide deletion of this gene is reported in HCC for the first time.Mutation in MTS1/p16 is found only in one tumor case.We do not find mutations in CDH1/E-cadherin.Furthermore,a statistically significant correlation is present between p53 gene mutation and loss of chromosome region 16q21-q23 and 9p21-p23,which indicates that synergism between p53 inactivation and deletion of 16q21-q23 and 9p21-p23 may play a role in the pathogenesis of HCC.     

Characterization of glucose-6-phosphate dehydrogenase deficiency and identification of a novel haplotype 487G>A/IVS5-612(G>C) in the Achang population of southwestern China

The prevalence of glucose-6-phosphate dehydrogenase (G6PD) deficiency and its gene mutations were studied in the Achang population from Lianghe County in Southwestern China. We found that 7.31% (19 of 260) males and 4.35% (10 of 230) females had G6PD deficiency. The molecular analysis of G6PD gene exons 2―13 was performed by a PCR-DHPLC-Sequencing or PCR-Sequencing. Sixteen inde-pendent subjects with G6PD Mahidol (487G>A) and the new polymorphism IVS5-612 (G>C), which combined into a novel haplotype, were identified accounting for 84.2% (16/19). And 100% Achang G6PD Mahidol were linked to the IVS5-612 C. The percentage of G6PD Mahidol in the Achang group is close to that in the Myanmar population (91.3% 73/80), which implies that there are some gene flows between Achang and Myanmar populations. Interestingly, G6PD Canton (1376G>T) and G6PD Kaiping (1388G>A), which were the most common G6PD variants from other ethnic groups in China, were not found in this Achang group, suggesting that there are different G6PD mutation profiles in the Achang group and other ethnic groups in China. Our findings appear to be the first documented report on the G6PD genetics of the AChang people, which will provide important clues to the Achang ethnic group origin and will help prevention and treatment of malaria in this area.     

云南保山猪线粒体DNA D-loop区序列初步分析 Primary Analysis of Mitochondrial DNA D-loop Region Sequence in Baoshan Pig

摘要：为了解云南保山猪(Baoshan pig)的遗传多样性及其遗传背景,我们测定了19个个体线粒体DNA D-loop高变区I 15 363～15 801片段序列438 bp。检测到10种单倍型,包括8个多态位点,其中5次T/C转换、1次G/A转换、1次G/C颠换和1次A/T颠换,其A、T、G、C碱基的平均含量分别为35.4%、26.9%、13.2%和24.5%,A+T含量(62.3%)明显高于G+C含量(37.7%)。对于保山猪的保种及其持续利用有着重要的理论指导意义。 Abstract:To investigate the genetic diversity and genetic data of Baoshan pig in Yunnan province,the mitochondrial DNA D-loop hypervariable segment I sequences 15 363～15 801 (438 bp) in 19 individuals of Baoshan pig were sequenced.Ten mitochondrial haplotypes were identified in the samples,with 8 sites showing polymorphism,which were 5 T/C and 1 G/A transitions,1 G/C and 1 A/T transversions.The contents of A,T,G and C were 35.4%,269%,13.2% and 24.5%,respectively.The content of A+T (62.3%) was significantly higher than that of G+C (37.3%).It will be of importance to conservation and sustainable utilization in Baoshan pig.     

Cells in G_2/M phase increased in human nasopharyngeal carcinoma cell line by EBV-LMP1 through activation of NF-кB and AP-1

Although previous studies showed that the principal oncoprotein encoded by Epstein-Barr virus, latentmembrane protein 1 (LMP1) 5 could induce the nasopharyngeal carcinoma cells in G_2/M phase increased, littleis known about the target molecules and mechanisms. The present study demonstrated that LMP1 couldinduce the accumulation of p53 protein and upregulate its transactivity in a dose dependent manner, whichresulted in the decrease of the kinase activity of cdc2/cyclin B complex and inducing arrest at G2/M phasethrough the activation of NF-κB and AP-1 signaling pathways, and the effect of NF-κB was more obviousthan that of AP-1. This study provided some significant evidence for further elucidating the molecularmechanisms that LMP1 had effects on the surveillance mechanism of cell cycle and promoting the survivalof transformed cells and tumorigenesis.     

汉族左利人群的指纹研究

对111名长治市的汉族左利手者的指纹进行了分析研究, 并与138名右利手者组成的对照组进行了对比分析, 发现左利手组的双箕斗纹（Wd）显著高于对照组(P<0.005)。 Abstract　To study the distribution difference of fingerprint patterns between left-handed and normal populations of Han nationality in Changzhi area, We investigated the frequencies of fingerprint pattern combinations in one or two hands. Comparing the normal population (80 men and 58 women), the left-handed population (58 men and 53 women) posessed higher Wdrate (P<0.005).     

Molecular characterization and expression analysis of Triticum aestivum squamosa-promoter binding protein-box genes involved in ear development

Wheat(Triticum aestivum L.) is one of the most important crops in the world. Squamosa-promoter binding protein(SBP)-box genes play a critical role in regulating flower and fruit development. In this study, 10 novel SBP-box genes(TaSPL genes) were isolated from wheat((Triticum aestivum L.) cultivar Yanzhan 4110). Phylogenetic analysis classified the TaSPL genes into five groups(G1–G5). The motif combinations and expression patterns of the TaSPL genes varied among the five groups with each having own distinctive characteristics: TaSPL20/21 in G1 and TaSPL17 in G2 mainly expressed in the shoot apical meristem and the young ear, and their expression levels responded to development of the ear; TaSPL6/15 belonging to G3 were upregulated and TaSPL1/23 in G4 were downregulated during grain development; the gene in G5(TaSPL3) expressed constitutively. Thus, the consistency of the phylogenetic analysis, motif compositions, and expression patterns of the TaSPL genes revealed specific gene structures and functions.On the other hand, the diverse gene structures and different expression patterns suggested that wheat SBP-box genes have a wide range of functions. The results also suggest a potential role for wheat SBP-box genes in ear development.This study provides a significant beginning of functional analysis of SBP-box genes in wheat.