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1.
The root hemiparasitic weed Striga hermonthica is a serious constraint to grain production of economically important cereals in sub-Saharan Africa. Breeding for parasite resistance in cereals is widely recognized as the most sustainable form of long-term control; however, advances have been limited owing to a lack of cereal germplasm demonstrating postattachment resistance to Striga. Here, we identify a cultivar of rice (Nipponbare) that exhibits strong postattachment resistance to S. hermonthica; the parasite penetrates the host root cortex but does not form parasite-host xylem-xylem connections. In order to identify the genomic regions contributing to this resistance, a mapping population of backcross inbred lines between the resistant (Nipponbare) and susceptible (Kasalath) parents were evaluated for resistance to S. hermonthica. Composite interval mapping located seven putative quantitative trait loci (QTL) explaining 31% of the overall phenotypic variance; a second, independent, screen confirmed four of these QTL. Relative to the parental lines, allelic substitutions at these QTL altered the phenotype by at least 0.5 of a phenotypic standard deviation. Thus, they should be regarded as major genes and are likely to be useful in breeding programmes to enhance host resistance. 相似文献
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Two trials were conducted to evaluate the efficacy of a granular mycoherbicide formulation based on Fusarium oxysporum and post-emergence herbicide for the control of the parasitic plant, Striga hermonthica in the Nigerian Savanna. Four fungal treatments were used: F. oxysporum followed by 2,4-D, F. oxysporum followed by supplementary hoe weeding, F. oxysporum followed by Triclopyr and a control (No F. oxysporum but hoe-weeded). The experiments were laid out in a split plot design with three replications in the two locations. The two varieties (Across 97 TZL and farmer's local variety) formed the main plot treatments, while the Striga fungal treatments formed the sub-plot treatments. At the Lafia location, the emergence of Striga was delayed by 7 days (46 days) as compared to the Makurdi location, which germinated earlier 39 days after sowing. Maize variety Across 97 TZL similarly delayed the time to Striga emergence when compared to the farmer's local variety. However, the different Striga control methods did not have any significant effect on the time of Striga emergence. Generally, number of maize plants infected with Striga was highest with the farmer's local variety throughout the period of observation, while in the Striga control treatments, hoe-weeded check recorded the highest; the minimum was obtained with plots treated with F. oxysporum followed by postemergence application of Triclopyr at the rate of 0.36 kg a.i./ha at 6 weeks after sowing. Highest maize grain yields were obtained at Lafia with Across 97 TZL and plots treated with F. oxysporum followed by either post-emergence 2,4-D or Triclopyr at 0.36 kg a.i./ha each. The results demonstrate the high potentiality of using F. oxysporum as a spot application at planting followed by post-emergence herbicide (2,4-D or Triclopyr) application at 6 weeks after sowing for the control of the parasitic plant S. hermonthica in the Nigerian Savanna. 相似文献
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A novel functional gene associated with cold tolerance at the seedling stage in rice 总被引:1,自引:0,他引:1
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Junliang Zhao Shaohong Zhang Jingfang Dong Tifeng Yang Xingxue Mao Qing Liu Xiaofei Wang Bin Liu 《Plant biotechnology journal》2017,15(9):1141-1148
Identification and cloning of cold‐tolerant genes that can stably express under different cold environments are crucial for molecular rice breeding for cold tolerance. In the previous study, we identified a cold‐tolerant QTL at the seedling stage, qCTS‐9 which could be detected under different cold environments using a recombinant inbred line (RIL) population derived from a cold‐tolerant variety Lijiangxintuanheigu (LTH) and a cold‐sensitive variety Shanhuangzhan 2 (SHZ‐2). In this study, eight candidate genes within the qCTS‐9 interval were identified through integrated analysis of QTL mapping with genomewide differential expression profiling of LTH. The qRT‐PCR assay showed that only Os09g0410300 exhibited different expression patterns between LTH and SHZ‐2 during cold stress, and significantly positive correlation was found between cold induction of Os09g0410300 and seedling cold tolerance in the RI lines. Five SNPs and one InDel in the promoters of Os09g0410300 were detected between LTH and SHZ‐2, and the InDel marker ID410300 designed based on the insertion–deletion polymorphism in the promoter was significantly associated with seedling cold tolerance in RIL population. Further, Os09g0410300 over‐expression plants exhibited enhanced cold tolerance at the seedling stage compared with the wild‐type plants. Thus, our results suggest that Os09g0410300 is the functional gene underlying qCTS‐9. To our knowledge, it is a novel gene contributed to enhance cold tolerance at the seedling stage in rice. Identification of the functional gene underlying qCTS‐9 and development of the gene‐specific marker will facilitate molecular breeding for cold tolerance at the seedling stage in rice through transgenic approach and marker‐assisted selection (MAS). 相似文献
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Liangyong Ma Changdeng Yang Dali Zeng Jing Cai Ximing Li Zhijuan Ji Yingwu Xia Qian Qian Jinsong Bao 《遗传学报》2009,36(5):297-304
Simultaneous heading of plants within the same rice variety, also refer to heading synchrony, is an important factor that affects simultaneous ripening of the variety. Understanding of the genetic basis of heading synchrony may contribute to molecular breeding of rice with simultaneous heading and ripening. In the present study, a doubled haploid (DH) population, derived from a cross between Chunjiang 06 and TN1 was used to analyze quantitative trait locus (QTL) for heading synchrony related traits, i.e., early heading date (EHD), late heading date (LHD), heading asynchrony (HAS), and tiller number (PN). A total of 19 QTLs for four traits distributed on nine chromosomes were detected in two environments. One QTL, qHAS-8 for HAS, explained 27.7% of the phenotypic variation, co-located with the QTLs for EHD and LHD, but it was only significant under long-day conditions in Hangzhou, China. The other three QTLs, qHAS-6, qHAS-9, and qHAS-10, were identified under short-day conditions in Hainan, China, each of which explained about 11% of the phenotypic variation. Two of them, qHAS-6 and qHAS-9, were co-located with the QTLs for EHD and LHD. Two QTLs, qPN-4 and qPN-5 for PN, were detected in Hangzhou, and qPN-5 was also detected in Hainan. However, none of them was co-located with QTLs for EHD, LHD, and HAS, suggesting that PN and HAS were controlled by different genetic factors. The results of this study can be useful in marker assisted breeding for improvement of heading synchrony. 相似文献
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Statistical methods established for the genetic analysis of quantitative traits can be applied to gene expression data. Quantitative trait locus (QTL) analysis can associate the expression of genes or groups of genes with particular genomic regions, and thereby identify regions regulating gene expression. A segregating population of 41 doubled haploid (DH) lines from the hard red spring wheat cross RL4452 x 'AC Domain' was used to map expression level polymorphisms. This population had previously been mapped with microsatellites, and includes a full QTL analysis for agronomic and seed quality traits. Expression analysis on mRNA from developing seed grown in two field locations was conducted on 39 of the 41 DH lines using the Affymetrix GeneChip Wheat Genome Array. Analysis of the hybridization intensity identified 1484 Affymetrix probe sets in the first location and 10,280 probe sets in the second location, where the hybridization intensity varied significantly between genotypes of the population. A common set of 1455 probe sets differing in intensity between genotypes in both locations was used for mapping, and 542 QTLs were identified that each mapped to a single chromosome interval, illustrating that major gene expression QTLs could be found in wheat. Genomic regions corresponding to multiple gene expression QTLs were identified. Comparison of expression mapping data with physical mapping of wheat expressed sequence tag (EST) sequences using rice synteny, as well as logarithm of odds (LOD) score analysis, showed that both cis- and trans-acting expression QTLs were present. Chromosomes 1D and 4B may contain significant trans-regulatory regions in this population. 相似文献
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《Journal of Plant Interactions》2013,8(1):315-323
The fungal disease, charcoal root rot, caused by Macrophomina phaseolina is a foremost yield restraining factor of Sorghum bicolor L. around the world. The expression analysis of genes induced in general defense response can endow with clues to reveal major defense mechanisms against pathogen infection in sorghum plant. The role of chitinase and Stilbene synthase in response to M. phaseolina in sorghum was studied under control growth conditions using a real-time polymerase chain reaction. Here, we report the expression analysis of antifungal genes in two cultivars viz. PJ-1430 (resistant) and SU-1080 (susceptible) at different hours after inoculation with M. phaseolina isolate MTCC 2165. Chitinase and stilbene synthase were induced in PJ-1430 within 0 h, 24 h and in SU-1080 in 48 h, 24 h, respectively, after inoculation. However, the expression levels of chitinase and stilbene synthase in resistant cultivar were significantly higher. The results showed that chitinase and stilbene synthase can be effective to enhance resistance to M. phaseolina. 相似文献
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Javad Nadaf Cecile Berri Ian Dunn Estelle Godet Elisabeth Le Bihan-Duval Dirk Jan De Koning 《Genetics》2014,196(3):867-874
Genetical genomics has been suggested as a powerful approach to study the genotype–phenotype gap. However, the relatively low power of these experiments (usually related to the high cost) has hindered fulfillment of its promise, especially for loci (QTL) of moderate effects.One strategy with which to overcome the issue is to use a targeted approach. It has two clear advantages: (i) it reduces the problem to a simple comparison between different genotypic groups at the QTL and (ii) it is a good starting point from which to investigate downstream effects of the QTL. In this study, from 698 F2 birds used for QTL mapping, gene expression profiles of 24 birds with divergent homozygous QTL genotypes were investigated. The targeted QTL was on chromosome 1 and affected initial pH of breast muscle. The biological mechanisms controlling this trait can be similar to those affecting malignant hyperthermia or muscle fatigue in humans. The gene expression study identified 10 strong local signals that were markedly more significant compared to any genes on the rest of the genome. The differentially expressed genes all mapped to a region <1 Mb, suggesting a remarkable reduction of the QTL interval. These results, combined with analysis of downstream effect of the QTL using gene network analysis, suggest that the QTL is controlling pH by governing oxidative stress. The results were reproducible with use of as few as four microarrays on pooled samples (with lower significance level). The results demonstrate that this cost-effective approach is promising for characterization of QTL. 相似文献
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Diverse RNA viruses elicit the expression of common sets of genes in susceptible Arabidopsis thaliana plants 总被引:6,自引:0,他引:6
Whitham SA Quan S Chang HS Cooper B Estes B Zhu T Wang X Hou YM 《The Plant journal : for cell and molecular biology》2003,33(2):271-283
Systemic infections of plants by viruses require that viruses modify host cells in order to facilitate infections. These modifications include induction of host factors required for replication, propagation and movement, and suppression of host defense responses, which are likely to be associated with changes in host gene expression. Past studies of the effects of viral infection on gene expression in susceptible hosts have been limited to only a handful of genes. To gain broader insight into the responses elicited by viruses in susceptible hosts, high-density oligonucleotide probe microarray technology was used. Arabidopsis leaves were either mock inoculated or inoculated with cucumber mosaic cucumovirus, oil seed rape tobamovirus, turnip vein clearing tobamovirus, potato virus X potexvirus, or turnip mosaic potyvirus. Inoculated leaves were collected at 1, 2, 4, and 5 days after inoculation, total RNA was isolated, and samples were hybridized to Arabidopsis GeneChip microarrays (Affymetrix). Microarray hybridization revealed co-ordinated changes in gene expression in response to infection by diverse viruses. These changes include virus-general and virus-specific alterations in the expression of genes associated with distinct defense or stress responses. Analyses of the promoters of these genes further suggest that diverse RNA viruses elicit common responses in susceptible plant hosts through signaling pathways that have not been previously characterized. 相似文献
10.
玉米5个农艺性状的QTL定位 总被引:35,自引:0,他引:35
利用“豫玉22”构建的266个玉米F2:3家系为材料,通过一年两点的随机区组田间试验和分子标记分析,研究了玉米穗位高、雄穗分支数、茎粗、抽雄期、吐丝期5个重要农艺性状。相关分析表明,穗位高、雄穗分支数、茎粗与单株产量显著正相关,抽雄期与吐丝期高度正相关,雄穗分支数与茎粗显著正相关。采用复合区间作图法,通过500次排列测验分别确定各性状的LOD阈值,在武汉和襄樊两地共定位了7个穗位高QTL、9个雄穗分支数QTL、8个茎粗QTL、9个抽雄期QTL和7个吐丝期QTL;这些QTL在染色体上分布不均匀,具有集中分布的特点。研究表明,数量性状间的表型相关可能源于控制数量性状的QTL位点间的相关。 相似文献
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Dissection of genetic overlap of salt tolerance QTLs at the seedling and tillering stages using backcross introgression lines in rice 总被引:3,自引:0,他引:3
JinPing Zang Yong Sun Yun Wang Jing Yang Fang Li YongLi Zhou LingHua Zhu Reys Jessica Fotokian Mohammadhosein JianLong Xu ZhiKang Li 《中国科学:生命科学英文版》2008,51(7):583-591
QTLs for salt-tolerance(ST)related traits at the seedling and tillering stages were identified using 99 BC2F8 introgression lines(IL)derived from a cross between IR64(indica)as a recurrent parent and Binam(japonica)from Iran as the donor parent.Thirteen QTLs affecting survival days of seedlings(SDS), score of salt toxicity of leaves(SST),shoot K concentration(SKC)and shoot Na concentration(SNC) at the seedling stage and 22 QTLs underlying fresh weight of shoots(FW),tiller number per plant(TN) and plant height(PH)at the tillering stage were identified.Most QTLs detected at the tillering stage showed obvious differential expression to salt stress and were classified into three types based on their differential behaviors.Type I included 11 QTLs which were expressed only under the non-stress condition.Type II included five QTLs expressed in the control and the salt stress conditions,and three of them(QPh5,QPh8 and QTn9)had similar quantity and the same direction of gene effect,suggesting their expression was less influenced by salt stress.Type III included six QTLs which were detectable only under salt stress,suggesting that these QTLs were apparently induced by the stress.Thirteen QTLs affecting trait difference or trait stability of ILs between the stress and non-stress conditions were identified and the Binam alleles at all loci except QPh4,QTn2 and QFw2a decreased trait difference.The three QTLs less influenced by the stress and 13 QTLs affecting trait stability were considered as ST QTLs which contributed to ST.Comparing the distribution of QTLs detected at the seedling and tillering stages,most(69%)of them were genetically independent.Only four were the same or adjacent regions on chromosomes 1,2,8 and 11 harboring ST QTLs detected at the two stages,suggesting that partial genetic overlap of ST across the two stages occurs.It is likely,therefore,to develop ST rice variety for both stages by pyramiding of ST QTLs of different stages or selection against the overlapping QTLs between the two stages via marker-assisted selection(MAS). 相似文献
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Guohua Yang Sansi Tu Shaoqing Li Lingling Feng Jin Kong Hui Li Yangsheng Li 《生物学前沿》2008,3(4):443-448
A population of 117 doubled haploid (DH) lines derived from the cross of Zhaiyeqing 8 (indica) x Jingxi 17 (japonica) was employed to map quantitative trait loci (QTL) underlying four physiological traits related to chlorophyll contents of the flag leaf. There were significantly positive correlations among chlorophyll a, chlorophyll b and chlorophyll a+ b content. Chlorophyll a/b ratio was significantly negatively correlated with chlorophyll b content. These four traits were normally distributed with transgressive segregation, suggesting that they were controlled by multiple minor genes. A total of 11 QTLs were detected for the four traits and they lay on six chromosomes. Each of them explained 9.2%-19.6% of the phenotypic variations, respectively. Of these, two QTLs controlling chlorophyll a content were mapped on chromosomes 2 and 5; four QTLs underlying chlorophyll b content were mapped on chromosomes 2, 3, 5 and 9; three QTLs underlying chlorophyll a+b amount were mapped on chromosomes 3, 5 and 9; two QTLs under-lying chlorophyll a/b ratio were mapped on chromosomes 6 and 1 1. The intrinsic relationship among the four traits and the practical implication in rice breeding are discussed. 相似文献
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Guohua Yang Sansi Tu Shaoqing Li Lingling Feng Jin Kong Hui Li Yangsheng Li 《Frontiers of Biology in China》2008,3(4):443-448
A population of 117 doubled haploid (DH) lines derived from the cross of Zhaiyeqing 8 (indica)× Jingxi 17 (japonica) was employed to map quantitative trait loci (QTL) underlying four physiological traits related to chlorophyll contents of
the flag leaf. There were significantly positive correlations among chlorophyll a, chlorophyll b and chlorophyll a + b content.
Chlorophyll a/b ratio was significantly negatively correlated with chlorophyll b content. These four traits were normally
distributed with transgressive segregation, suggesting that they were controlled by multiple minor genes. A total of 11 QTLs
were detected for the four traits and they lay on six chromosomes. Each of them explained 9.2%–19.6% of the phenotypic variations,
respectively. Of these, two QTLs controlling chlorophyll a content were mapped on chromosomes 2 and 5; four QTLs underlying
chlorophyll b content were mapped on chromosomes 2, 3, 5 and 9; three QTLs underlying chlorophyll a + b amount were mapped
on chromosomes 3, 5 and 9; two QTLs underlying chlorophyll a/b ratio were mapped on chromosomes 6 and 11. The intrinsic relationship
among the four traits and the practical implication in rice breeding are discussed.
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Translated from Journal of Wuhan University (Science Edition), 2006, 52(6): 751–756 [译自: 武汉大学学报(理学版)] 相似文献
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Upland rice (Oryza sativa L.) was grown at both ambient (350 μmol mol?1) and elevated (700 μmol mol?1) CO2 in either the presence or absence of the root hemi‐parasitic angiosperm Striga hermonthica (Del) Benth. Elevated CO2 alleviated the impact of the parasite on host growth: biomass of infected rice grown at ambient CO2 was 35% that of uninfected, control plants, while at elevated CO2, biomass of infected plants was 73% that of controls. This amelioration occurred despite the fact that O. sativa grown at elevated CO2 supported both greater numbers and a higher biomass of parasites per host than plants grown at ambient CO2. The impact of infection on host leaf area, leaf mass, root mass and reproductive tissue mass was significantly lower in plants grown at elevated as compared with ambient CO2. There were significant CO2 and Striga effects on photosynthetic metabolism and instantaneous water‐use efficiency of O. sativa. The response of photosynthesis to internal [CO2] (A/Ci curves) indicated that, at 45 days after sowing (DAS), prior to emergence of the parasites, uninfected plants grown at elevated CO2 had significantly lower CO2 saturated rates of photosynthesis, carboxylation efficiencies and ribulose‐1,5‐bisphosphate carboxylase/oxygenase (Rubisco; EC 4.1.1.39) contents than uninfected, ambient CO2‐grown O. sativa. In contrast, infection with S. hermonthica prevented down‐regulation of photosynthesis in O. sativa grown at elevated CO2, but had no impact on photosynthesis of hosts grown at ambient CO2. At 76 DAS (after parasites had emerged), however, infected plants grown at both elevated and ambient CO2 had lower carboxylation efficiencies and Rubisco contents than uninfected O. sativa grown at ambient CO2. The reductions in carboxylation efficiency (and Rubisco content) were accompanied by similar reductions in nitrogen concentration of O. sativa leaves, both before and after parasite emergence. There were no significant CO2 or infection effects on the concentrations of soluble sugars in leaves of O. sativa, but starch concentration was significantly lower in infected plants at both CO2 concentrations. These results demonstrate that elevated CO2 concentrations can alleviate the impact of infection with Striga on the growth of C3 hosts such as rice and also that infection can delay the onset of photosynthetic down‐regulation in rice grown at elevated CO2. 相似文献
16.
Using SRAP (sequence-related amplified polymorphism) markers a genetic linkage map of cucumber was constructed with a population
consisting of 138 F2 individuals derived from a cross of the two cucumber lines, S06 and S52. In the survey of parental polymorphisms with 182
primer combinations, 64 polymorphism-revealing primer pairs were screened out, which generated totally 108 polymorphic bands
with an average of 1.7 bands per primer pair and at most 6 bands from one primer pair. The constructed molecular linkage map
included 92 loci, distributed in seven linkage groups and spanning 1164.2 cM in length with an average genetic distance of
12.6 cM between two neighboring loci. Based on this linkage map, the quantitative trait loci (QTL) for the lateral branch
number (lbn) and the lateral branch average length (lbl) in cucumber were identified by QTLMapper1.6. A major QTL lbn1 located
between ME11SA4B and ME5EM5 in LG2 could explain 10.63% of the total variation with its positively effecting allele from S06.
A major QTL lbl1 located between DC1OD3 and DC1EM14 in LG2 could account for 10.38% of the total variation with its positively
effecting allele from S06. 相似文献
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Physical map of the wheat high-grain protein content gene Gpc-B1 and development of a high-throughput molecular marker 总被引:1,自引:0,他引:1
Grain protein content (GPC) is important for human nutrition and has a strong influence on pasta and bread quality. A quantitative trait locus, derived from a Triticum turgidum ssp. dicoccoides accession (DIC), with an average increase in GPC of 14 g kg(-1) was mapped on chromosome 6BS. Using the wheat-rice colinearity, a high-density map of the wheat region was developed and the quantitative trait locus was mapped as a simple Mendelian locus designated Gpc-B1. A physical map of approx. 250 kb of the Gpc-B1 region was developed using a tetraploid wheat bacterial artificial chromosome library. The constructed physical map included the two Gpc-B1 flanking markers and one potential candidate gene from the colinear rice region completely linked to Gpc-B1. The relationship between physical and genetic distances and the feasibility of isolating genes by positional cloning in wheat are discussed. A high-throughput codominant marker, Xuhw89, was developed. A 4-bp deletion present in the DIC allele was absent in a collection of 117 cultivated tetraploid and hexaploid wheat germplasm, suggesting that this marker will be useful to incorporate the high GPC allele from the DIC accession studied here into commercial wheat varieties. 相似文献
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In populations derived from commercial hybrid rice combination Shanyou 10, F1 hetero-sis and F2 inbreeding depression were observed on grain yield (GYD) and number of panicles (NP). Using marker loci evenly distributed on the linkage map as fixing factors, the F2 population was divided into sub-populations. In a large number of sub-populations, significant correlations were observed between heterozygosity and GYD, and between heterozygosity and NP. This was especially true in type III sub-populations in which the genotype of a fixing factor was heterozy-gotes. In type III sub-populations, 15 QTL for GYD and 13 QTL for NP were detected, of which the majority exhibited over-dominance effects for increasing the trait values. This study showed that over-dominance played an important role in the genetic control of heterosis in rice. 相似文献
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O. Matika R. Pong‐Wong R. D. Houston A. Clop J. A. Woolliams S. C. Bishop 《Animal genetics》2011,42(2):172-180
This study aimed at verifying previously identified QTL affecting growth and carcass traits on ovine chromosome 18 (OAR18) in Texel sheep (n = 1844), and on OAR1 in Charollais (n = 851) and Suffolk (n = 998) sheep. The QTL were investigated using regression and variance component mapping (VCA) of body weight, muscle and fat depth measurements. In addition, the mode of inheritance of the Texel OAR18 QTL was explored, using data from 4376 Texel sheep, fitting VCA models testing for additive and imprinting effects. We also simulated a 480‐sheep population with different QTL imprinting models and various available levels of marker information to understand the behaviour of the VCA results under different assumed genetic models. In summary, the previously identified QTL were successfully verified using both interval mapping and VCA in the three breeds. We propose a polar overdominance mode of inheritance for the OAR18 QTL in Texel sheep, and we present methods to dissect the QTL mode of inheritance, using the Texel OAR18 QTL as an example. 相似文献