首页 | 本学科首页   官方微博 | 高级检索  
相似文献
 共查询到20条相似文献,搜索用时 23 毫秒
1.
Summary During embryogenesis and planula development of the colonial hydroidHydractinia echinata cell proliferation decreases in a distinct spatio-temporal pattern. Arrest in S-phase activity appears first in cells localized at the posterior and then subsequently at the anterior pole of the elongating embryo. These areas do not resume S-phase activity, even during the metamorphosis of the planula larva into the primary polyp. Tissue containing the quiescent cells gives rise to the terminal structures of the polyp. The posterior area of the larva becomes the hypostome and tentacles, while the anterior part of the larva develops into the basal plate and stolon tips. In mature planulae only a very few cells continue to proliferate. These cells are found in the middle part of the larva. Labelling experiments indicate that the prospective material of the postmetamorphic tentacles and stolon tips originates from cells which have exited from the cell cycle in embryogenesis or early in planula development. Precursor cells of the nematocytes which appear in the tentacles of the polyp following metamorphosis appear to have ceased cycling before the 38th hour of embryonic development. The vast majority of the cells that constitute the stolon tips of the primary polyp leave the cell cycle not later than 58 h after the beginning of development. We also report the identification of a cell type which differentiates in the polyp without passing through a post-metamorphic S-phase. The cell type appears to be neural in origin, based upon the identification of a neuropeptide of the FMRFamide type.  相似文献   

2.
In the marine hydroid Hydractinia echinata, metamorphosis transforms the spindle-shaped larva into a primary polyp. It bears a hypostome with a ring of tentacles at its apical end, a gastric region in the middle and stolons at the base. In nature, metamorphosis is induced in response to external stimuli provided by bacteria. These stimuli can be replaced by artificial inducers, one of which is heat shock. Among heat shock treated stages are those undergoing complete metamorphosis but also specimens forming chimeras of different developmental stages. In the chimeric larvae, the posterior is transformed into the apical hypostome of the adult polyp while the anterior part of the larva persists as larval tissue. After transverse sectioning, these stage chimeras regenerate the missing body parts with respect to the nature of the tissue at the wound surface. This shows that the decision to make larva or polyp morphology depends not on the majority of the tissue in the original body section, but on stage specificity within the regenerating animal part. Single cells can escape from this general rule, since RFamide nerve cells which usually differentiate in polyp tissue appear in regenerated larval tails of sectioned stage chimeras. The results indicate that the pattern-forming system of the larva and of the adult have features in common. The primary signals controlling patterning along the anterior-posterior axis in larvae and the apical-basal axis in polyps arethus likelyto be the same while the interpretation of these primary signals by the individual cells changes during metamorphosis.  相似文献   

3.
Summary Antisera to the sequence Arg-Phe-amide (RF-amide) have a high affinity to the nervous system of fixed hydroid polyps. Whole-mount incubations of several Hydra species with RFamide antisera visualize the three-dimensional structure of an ectodermal nervous system in the hypostome, tentacles, gastric region and peduncle. In the hypostome of Hydra attenuata a ganglion-like structure occurs, consisting of numerous sensory cells located in a region around the mouth opening and a dense plexus of processes which project mostly radially towards the bases of the tentacles. In Hydra oligactis an ectodermal nerve ring was observed lying at the border of hypostome and tentacle bases. This nerve ring consists of a few large ganglion cells with thick processes forming a circle around the hypostome. This is the first direct demonstration of a nerve ring in a hydroid polyp.Incubation of Hydractinia echinata gastrozooids with RFamide antisera visualizes an extremly dense plexus of neuronal processes in body and head regions. A ring of sensory cells around the mouth opening is the first group of neurons to show RFamide immunoreactivity during the development of a primary polyp. In gonozooids the oocytes and spermatophores are covered with strongly immunoreactive neurons.All examples of whole-mount incubations with RF-amide antisera clearly show that hydroid polyps have by no means a diffuse nerve net, as is often believed, and that neuronal centralization and plexus formation are common in these animals. The examples also show that treatment of intact fixed animals with RFamide antisera is a useful technique to study the anatomy or development of a principal portion of the hydroid nervous system.  相似文献   

4.
Eggs of medusae develop into lecithotrophic planulae that undergo metamorphosis at different ages to form polyps. As planulae age they decrease in size as their yolk stores are utilized. The planulae of most Phialidium medusae develop into polyps where there is a decrease in the size of the holdfast region and a relative increase in the size of the hydranth region as they age. These changes occur independently of the decrease in planula size. In planulae with a decrease in the size of the holdfast region and an increase in the size of the hydranth-forming region there was a 50% decline in polyps that successfully stayed attached to the substrate after metamorphosis. These aged planulae produced an initial hydranth with the same number of tentacles as polyps from full-sized young planulae while young half-sized planulae produced hydranths where the tentacle number was smaller. The first phase of polyp colony growth with a small initial hydranth was slower than growth of a colony with a larger initial hydranth. Predation during this period led to more death in colonies with a small initial hydranth. The decline in successful attachment in aged planulae was not offset by the higher rate of growth and a smaller window of time where predation leads to death, suggesting that this age-related developmental change in planulae was not adaptive.  相似文献   

5.
Plickert  G. 《Hydrobiologia》1991,216(1):83-89
Treatment of metamorphosing planulae of Hydractinia echinata with proportion altering factor (PAF) causes oversizing in the hypostome of developing primary polyps. With increasing concentrations more specimens fail to acquire the appearance of a primary polyp but remain as cones lacking tentacles. Yet, such specimens are true primary polyps as they contain neurons of RF-amide-like immunoreactivity that are typical of the polyp stage. Numbers of RF-amide-positive cells are increased up to 2.5 times compared with the normal value. In intact Hydra vulgaris, PAF increases the number of neurons that differentiate from cycling precursor cells, or even from stem cells. The effect is dose dependent.  相似文献   

6.
The life cycle ofTripedalia cystophora includes a sessile saclike polyp — the asexual reproducing form — and a pelagic tetraradial medusa — the sexually reproducing generation. Medusan development can be induced by temperature increase. It reveals neither budding nor strobilation, but a real metamorphosis of a polyp to only one medusa. According to morphological and anatomical criteria the metamorphosis can be subdivided into four different stages: (1) four longitudinal furrows segment the polyp, the tentacles of which are apportionated on the four quadrants of the body. (2) The subumbrellar cavity develops by invagination of the peristom; the relicts of the fused tentacles change to four rhopalia buds. (3) Medusan architecture including four new interradial tentacles, four rhopalia and the subumbrellar swimming musculature is completed. (4) A young tetraradial medusa starts swimming. Ultrastructural analysis of those metamorphic stages show the different processes of morphogenesis: (a) Gastrodermal cells — absorptive and spumous cells — undergo transdifferentiation and proliferation to medusan cells of the same structure and function. (b) Epidermal cells, excluding the epithel muscle cells, dissociate and are autolytically withdrawn. Dedifferentiated epithel muscle cells — interstitial cells — regain the ability to develop a complete new set of somatic cells, not originally present in the polyp. They include amongst others cross-striated muscle cells, medusan typic nematocyts and particularly sensory and nervous cells. Those cells establish a nervous system with lens-eyes, simple ocelli, statocysts, diffuse nerve net and an additional nerve ring.  相似文献   

7.
Summary Patterning processes during embryonic development of Hydractinia echinata were analysed for alterations in morphology and physiology as well as for changes at the cellular level by means of treatment with proportioning altering factor (PAF). PAF is an endogenous factor known to change body proportions and to stimulate nerve cell differentiation in hydroids (Plickert 1987, 1989). Applied during early embryogenesis, this factor interferes with the proper establishment of polarity in the embryo. Instead of normal shaped planulae with one single anterior and one single posterior end, larvae with multiple termini develop. Preferentially, supernumerary posterior ends, which give rise to polyp head structures during metamorphosis, form while anterior ends are reduced. The formation of such polycaudal larvae coincide with an increase in the number of interstitial cells and their derivatives at the expense of epithelial cells. Treatment of further advanced embryonic stages causes an increase in length, presumably due to the general stimulation of cell proliferation observed in such embryos. Also, the spatial arrangement of cells (i.e. cells in proliferation and RFamide (Arg-Phe-amide immunopositive nerve cells) is altered by PAF. Larvae that develop from treated embryos display altered physiological properties and are remarkably different from normal planulae with respect to their morphogenetic potential: (1) Larvae lose their capacity to regenerate missing anterior parts; isolated posterior larva fragments form regenerates of a bicaudal phenotype. (2) In accordance with the frequently observed reduction of anterior structures, the capacity to respond to metamorphosis-inducing stimuli decreases. (3) The morphogenetic potential to form basal polyp parts is found to be reduced. In contrast, the potential to form head structures during metamorphosis increases, since primary polyps with supernumerary hypostomes and tentacles metamorphose from treated animals.  相似文献   

8.
The structure of the larval nervous system and the musculature of Phoronis pallida were studied, as well as the remodeling of these systems at metamorphosis. The serotonergic portion of the apical ganglion is a U-shaped field of cell bodies that send projections into a central neuropil. The majority of the serotonergic cells are (at least) bipolar sensory cells, and a few are nonsensory cells. Catecholaminergic cell bodies border the apical ganglion. The second (hood) sense organ develops at competence and is composed of bipolar sensory cells that send projections into a secondary neuropil. Musculature of the competent larva includes circular and longitudinal muscle fibers of the body wall, as well as elevators and depressors of the tentacles and hood. The juvenile nervous system and musculature are developed prior to metamorphosis and are integrated with those of the larva. Components of the juvenile nervous system include a diffuse neural net of serotonergic cell bodies and fibers and longitudinal catecholaminergic fibers. The juvenile body wall musculature consists of longitudinal fibers that overlie circular muscle fibers, except in the cincture regions, where this pattern is reversed. Metamorphosis is initiated by the larval neuromuscular system but is completed by the juvenile neuromuscular system. During metamorphosis, the larval nervous system and the musculature undergo cell death, and the larval tentacles and gut are remodeled into the juvenile arrangement. Although the phoronid nervous system has often been described as deuterostome-like, these data show that several cytological aspects of the larval and juvenile neuromuscular systems also have protostome (lophotrochozoan) characteristics.  相似文献   

9.
The larvae of many marine organisms including hydrozoans are lecithotrophic and will not feed until after metamorphosis. In hydrozoans the aboral region of the planula becomes the holdfast and stolon, while the oral region becomes the stalk and hydranth that grows out of the holdfast following metamorphosis. If metamorphosis is delayed, the portion of the planula allocated to form holdfast and stolon shrinks and the region that forms the hydranth increases in size. Planulae also have the ability to regenerate their polyp prepattern. When the aboral region of the planula that does not normally form a hydranth is isolated and metamorphosis is delayed, it acquires the capacity to form a hydranth from the holdfast. A relatively high proportion of entodermal cells of young planulae engage in DNA synthesis (BrdU labeling index); as planulae age, the labeling index falls close to zero. When the polyp prepattern is modified during planula regeneration, entodermal cells are induced to engage in DNA synthesis. If DNA synthesis is inhibited in planulae, the polyp prepattern changes during regeneration and age-related developmental changes in planula are inhibited, suggesting that DNA synthesis is a necessary part of the pattern respecification process.  相似文献   

10.
FollowingHaeckel (1880), most zoologists have grouped the Cubomedusae with the class Scyphozoa. However, the actual systematic position and evolution of the Cubomedusae remained unclear because essential phenomena of the life cycle, i. e. life history and structure of the polyp generation and the process of medusa formation were unknown. Successful cultivation of the Carribean larviparousTripedalia cystophora Conant, 1898 elucidated for the first time the complete life cycle of a cubomedusa. Primary polyps could be raised from planulae which were transferred by air mail from La Parguera, Puerto Rico. The sessile polyp is solitary. Its morphology, anatomy, and behaviour are described. The body (length 0.6–1.0 mm) is radially constructed without any trace of tetramerous structures. 6 to 11 solid capitate tentacles insert in one circle, above which the body ends in a long contractile snout-like mouth cone (proboscis). The body is sac-like without gastric septa or gastric pockets; its base is enveloped by a small cup of thin, structureless periderm. Asexual reproduction by which the stock is enlarged quickly envolves lateral budding of small secondary polyps. After detachment these small polyps go through a creeping phase. The fully grown polyp shows a remarkable behavioural plasticity as it can migrate and change into an inactive encysted stage. The whole polyp metamorphoses into a single medusa. All externally visible metamorphosis phases are described. First, the polyp's body becomes tetramerous due to 4 longitudinal folds. The tentacles congregate into 4 groups, each in one quadrant. While the distal parts of the tentacles are resorbed, their bases develop into 4 perradial sensory organs (rhopalia). Interradially, 4 new tentacles are formed and become the primary tentacles of the medusa. Simultaneously, the complete body of the polyp transforms into the bell of the medusa. At the end of the metamorphosis which takes 5 to 6 days at 25 to 27° C, the young medusa begins to pulsate quickly and swims away leaving behind the empty peridermal cup. The morphology of the young medusa is described.T. cystophora has a tricnidom of basitrich haplonemes, holotrich haplonemes, and heterotrich microbasic euryteles. The ecology of both, polyp and medusa generation, is briefly outlined. A critical comparison between the polyp and medusa ofT. cystophora and the Scyphozoa and Hydrozoa reveals important differences. Consequently, a new class, Cubozoa, must be established and given the evolutionary position between Scyphozoa and Hydrozoa. Diagnoses are presented for the polyp ofT. cystophora and the class Cubozoa.  相似文献   

11.
Cells of scyphistomae, strobilae, and ephyrae were dissociated with trypsin and reaggregated. Clumping was inhibited in low Ca++ and by puromycin, but not by collagenase or sugars. Reaggregates from the oral end of the polyp developed tentacles and mouths first and basal structures later, whereas the opposite sequence occurred with cells from the lower gastric region. Nile-blue-stained cells from hypostome or peduncle did not form specific structures in the reconstructed polyp, but were distributed throughout the animal. Ephyra cell aggregates showed little morphogenesis, whereas cells from presumptive ephyra tissue gave rise to structures with tentacles and multiple oral openings. Mixed reaggregates containing equal proportions of polyp and ephyra cells formed irregular structures with transparent outer layer and opaque inner cell mass, suggesting stage-specific sorting.  相似文献   

12.
The larval development ofPhoronis psammophila Cori is divided into 6 phases (on the basis of increasing pairs of larval tentacles); furthermore an initial and a ripe phase are distinguished. Specific aspects of the development are described: Formation and structure of larval tentacles; anlage of adult tentacles as a thickening in the larval tentacle base; late development of the metasome (larva with 4–6 tentacles); formation of the metasome pouch in the larva with 8 tentacles; enlargement of the apical plate; differentiation of the gut; differentiation of larval nephridia; formation of pigment particles in the larva with 6 tentacles (storage function of pigments and its significance for larval identification); different types of discoflagella in various regions of the body. The larval development shows the following tendencies: Improvement of locomotion; intensification of food filtration; anlage of adult organs in the larva leading to a shortening of metamorphosis duration. The larva ofP. psammophila is compared with those ofP. pallida, P. hippocrepia, andP. vancouverensis. Earlier larval determinations ofP. psammophila (e.g.Actinotrocha sabatieri, A. hatschekii) are shown to have been mistakes. Termination of the postembryonic phase (metamorphosis) can be induced experimentally by bacteria and also by cations. Pure or mixed bacteria cultures must be present at the beginning exponential growth phase. The bacteria density required is 20–94×106 bact.ml?1 for pure cultures and on the average 28×106 bact. ml?1 for mixed cultures. Metamorphosis initiation by cations can be induced with CsCl (0.06 M) and RbCl (0.035 M). Metamorphosis ofP. psammophila occurs in 6 phases: larva, ready for metamorphosis; larva, activated by bacteria or ions; evagination of the metasome diverticle, dislocation of gut; losing and swallowing of episphaere and larval tentacles; formation of the youngP. psammophila. All developmental phases are described and compared with those ofP. muelleri; imperfect metamorphosis is characterized and the youngP. psammophila compared with older stages and the adult Phoronis.  相似文献   

13.
Summary Diffuse and synaptic nerve nets are present in the coenenchymal mesoglea and ectoderm of Muricea and Lophogorgia colonies. The nerve nets extend into the polyp column and tentacles maintaining a subectodermalmesogleal position. The density of nerve elements is low in comparison with similar nerve nets found in pennatulids.In the column of the polyp anthocodium, and throughout the oral disk region, neurons cross the mesoglea and enter the polyp endoderm. These neurons presumably connect with the endodermal nerve net which innervates the septal musculature. The trans-mesogleal neurons probably represent the connection between colonial and polyp nervous systems.In the tentacles, longitudinal ectodermal musculature is present with an overlying nerve plexus. These muscles and nerves, as well as tentacular sensory cells, are well represented in the oral side of the tentacles only.Presumed sensory cells form ciliary cone complexes in which one cell possesses an apical cilium. The other cells as well as the centrally located nematocyte contribute microvilli to the cone. The basal portion of the sensory cells is drawn into one or more neurite-like processes which enter the ectodermal nerve plexus. Similar processes form synapses with longitudinal muscle cells and nematocytes. The sensory cells of the ciliary cones presumably include chemoreceptors which can activate or modify nematocyst discharge, local muscle twitches, and tentacle bending.This work was supported by Office of Naval Research Contract N00014-75-C-0242, NSF Grant BMS 74-23242 and General Research Funds of the University of California, Santa Barbara. We wish to thank Dr. Steven K. Fisher for the use of facilities in his lab. This paper is part of a thesis to be submitted by R.A.S. to the Department of Biological Sciences, University of California, Santa Barbara in partial fulfillment of the requirements for the Ph. D.  相似文献   

14.
The hypostome and mouth of fresh-water Hydra were examined by scanning electron microscopy. The external surface of the hypostome possesses cnidocils, possibly sensory hairs, and small spiny protrusions surrounding the mouth; the internal surface has cylindrical microvilli, free flagella and adherent flagella. The adherent flagella are most numerous close to the mouth where they cause the cell surface to appear smooth when viewed at low magnifications. Free flagella and leaf-like microvilli increase in prominence towards the tentacles and enter on proper. The edge of the mouth has an abrupt boundary marking the apposition of epidermal and gastrodermal cells. A transitional groove occurs at the boundary and the cells underlying the groove are smaller than those on other regions of the hypostome. The transition groove may represent a site of cell loss in normal cell turnover. Some of the small underlying cells may represent nervous elements involved in regulating hypostome activity during the feeding reation.  相似文献   

15.
Planktonic larvae of the Athenaria correspond in a number of morphological characteristics to plankto-trophic thenarian planulae. In edwardsiid planulae there are often, during the later part of the planktonic phase, a well-developed aboral physa-"Anlage" and six triangular tentacles. Holotrichous haplonemes are not uncommon in actiniarian planulae. This nemato-cyst type is, however, limited to the larva stage in the edwardsiid and some thenarian species. The phylogenetical connection between the edwardsiid and the planktotrophic thenarian planulae is stressed The striking morphological resemblances between madreporarian and lecithotrophic planulae of the family Actiniidae indicate a close phylogenetic connection between the two planula types.  相似文献   

16.
Summary The scyphozoan polyp Cassiopea forms vegetative free swimming buds that metamorphose into sessile polyps. In sterile sea water metamorphosis does not take place. Buds keep swimming for weeks. Application of millimolar quantities of NH 4 + causes the buds to metamorphose within one day. The resulting animals bear hypostome and tentacles, however, only occasionally peduncle and foot. Almost all transform either completely into solitary polyp head or only the oral half of the bud developes into a head while the aboral half remains bud tissue which becomes constricted off. Under suited conditions this small bud is able to transform into a normal shaped polyp.  相似文献   

17.
Many marine invertebrates reproduce through a larval stage. The settlement and metamorphosis of most of the species are synchronised and induced by environmental organisms, mainly bacteria. The hydrozoan Hydractinia echinata has become a model organism for metamorphosis of marine invertebrates. In this species, bacteria, e.g. Pseudoalteromonas espejiana, are the natural inducers of metamorphosis. Like in other species of marine invertebrates, metamorphosis can be induced artificially by monovalent cations, e.g. Cs+. In this study, we present systematic data that metamorphosis—with both inducing compounds, the natural one from bacteria and the artificial one Cs+—are indeed similar with respect to (a) the morphological progression, (b) the localisation of the primary induction signal in the larva, (c) the pattern of apoptotic cells occurring during the initial 10 h of metamorphosis and (d) the disappearance of RFamide-dependent immunocytochemical signals in sensory neurons during this process. However, a difference occurs during the development of the anterior end, insofar as apoptotic cells and settlement appear earlier in planulae induced with bacteria. Thus, basically, Cs+ may be used as an artificial inducer, mimicking the natural process. However, differences in the appearance of apoptotic cells and in settlement raise the question of how enormous developmental plasticity in hydrozoans actually can be, and how this is related to the absence of malignant devolution in hydrozoans.  相似文献   

18.
Hydroid planulae metamorphose in response to an inducing external stimulus, usually a bacterial cue. There is evidence that neurotransmitters participate in the signal transduction pathway of hydroid metamorphosis. Eudendrium racemosum is a colonial hydroid common in the Mediterranean Sea. It lacks the medusa stage and the planulae develop on female colonies during the fertile season. In this work, serotonin (5-HT) was localized in some planula ectodermal cells. Co-localization of serotonin and beta-tubulin suggested that 5-HT was present in sensory nervous cells and in different ectodermal cells. To investigate the role of neurotransmitters in metamorphosis, E. racemosum planulae were treated with serotonin and dopamine and with agonists and antagonists of the corresponding receptors. Serotonin and a serotonin receptor agonist induced metamorphosis, while a 5-HT receptor antagonist inhibited it. Dopamine and all dopaminergic drugs used did not show any significant effect on the onset of metamorphosis. Results from this work showed that 5-HT could stimulate metamorphosis in E. racemosum planulae in the presence of a natural inducer. A mechanism by which this neurotransmitter could act in this phase is proposed.  相似文献   

19.
Electron microscopic study of an 18-day-old planulae and primary polyps of the sea pen, Ptilosarcus gurneyi, reveals 14 cell types: sustentacular cell A, sustentacular cell B, nerve cell, sensory cell, cnidoblast, interstitial cell, five types of gland cell (A, B, C, D and E), amoebocyte, style cell and endodermal cell. Of these, 9 are found in the planula, 12 in polyps and 7 are common to both stages. The fine structure of all cell types is described. Since the planulae and polyps in this study were identical in age of development, the gaining and losing of certain types of cells in the polyp are attributed to changes associated with settlement and metamorphosis. Modifications of the seven common cell types during metamorphosis can also be attributed to the change of life style from pelagic to benthic.  相似文献   

20.
采用扫描电镜、透射电镜和蛋白银染色等方法研究了海蜇胚胎发育和变态过程中细胞超微结构变化。结果显示: (1)海蜇自受精卵至原肠期阶段细胞均等分裂, 细胞间存在大量连接, 细胞形态相近, 未出现显著分化; (2)海蜇自早期浮浪游虫阶段, 其外胚层细胞开始出现空泡化, 至4触手螅状体阶段外胚层细胞空泡体积逐渐增大, 而内胚层细胞仅在4触手螅状体阶段才出现空泡化。伴随着外胚层细胞空泡化比例的增大, 杯状体和4触手螅状体阶段出现疑似凋亡小体结构; (3)刺细胞分化于早期浮浪游虫期的外胚层近中胶层区域, 而后逐渐向外转移, 至4触手螅状体阶段发育成熟并转移至表面; (4)纤毛形成于早期浮浪幼虫, 在杯状体阶段逐渐退化, 并于4触手螅状体阶段完全消失; (5)在海蜇早期发育各个阶段, 其内部均发现大量着色较深的卵黄体, 且在浮浪游虫阶段首次发现了海蜇外层细胞主动吞噬细菌现象, 表明海蜇早期发育营养来自内源性和外源性两部分。研究结果可为阐明刺胞动物早期发育模式提供依据。  相似文献   

设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号