Effect of Nitrogen Supply on the Nitrogen Use Efficiency of an Annual Herb, Helianthus annuus L.

Nitrogen use efficiency (NUE) is the product of nitrogen productivity (NP) and the mean residence time of nitrogen (MRT). Theory suggests that there should be a trade-off between both components,but direct experimental evidence is still scarce. To test this hypothesis, we analyzed the effect of varying nitrogen supply levels on NUEand its two components (NP, MRT) in Helianthus annuus L., an annual herb.The plants investigated were subjected to six nitrogen levels (0, 2, 4, 8, 16, and 32 g N/m2). Total plant production increased substantially with increasing nitrogen supply. Nitrogen uptake and loss also in creased with nitrogen supply. Nitrogen influx (rin) and outflux (rout) were defined as the rates of nitrogen uptake and loss per unit aboveground nitrogen, respectively. Both rin and rout increased with increasing nitrogen supply. In addition, rin was far higher than rout. Consequently, the relative rate of nitrogen incre ment (rin- rout) also increased with nitrogen supply. There were marked differences between treatments with respect to parameters related to the stress resistance syndrome: nitrogen pool size, leaf nitrogen concentration,and net aboveground productivity increased with nitrogen supply. Plants at high nitrogen levels showed a higher NP (the growth rate per unit aboveground nitrogen) and a shorter MRT (the inverse of rout), whereas plants at low nitrogen levels displayed the reverse pattern. Shorter MRT for plants at high nitrogen levels was caused by the abscission of leaves that contained relatively large fractions of total plant nitrogen. We found a negative relationship between NP and MRT, the components of NUE, along the gradient of nitrogen availability, suggesting that there was a trade-off between NP and MRT. The NUE increased with increasing nitrogen availability, up to a certain level, and then decreased. These results offer support for the hypoth esis that adaptation to infertile habitats involves a low nitrogen loss (long MRT in the plant) rather than a high NUE per se. The higher NUE at the plant level was a result, in part, of greater nitrogen resorption during senescence. We suggest that a long MRT (an index of nitrogen conservation) is a potentially successful strategy in nitrogen-poor environments.     

氮素形态及配比对夏播菘蓝生长及活性成分含量的影响

以来自山西的菘蓝(Isatis indigotica Fort.)为实验对象,采用盆栽法研究铵态氮(NH4+-N)、硝态氮(NO3--N)和酰胺态氮〔CO(NH2)2〕的不同配比对夏播菘蓝生长,叶和根中的可溶性蛋白质及总氮含量,根中多糖含量,叶中叶绿素相对含量,以及叶中靛玉红和靛蓝、根中(R,S)-告依春的含量和积累量的影响.结果表明:各施氮处理组的单株叶干质量均高于对照(不施用氮素)组,但单株根干质量或高于或低于对照组,其中,T4〔n(铵态氮):n(硝态氮):n(酰胺态氮)=25:75:0〕处理组的单株叶和根干质量均最大,且总体上显著高于对照组及其他施氮处理组(P<005);而施氮处理组的根冠比均显著低于对照组.各施氮处理组叶中的可溶性蛋白质含量与对照均无显著差异,但各施氮处理组根中的可溶性蛋白质含量、叶和根中的总氮含量以及叶中的叶绿素相对含量总体上显著高于对照组,而根中的多糖含量或高于或低于对照组,其中,T6〔n(铵态氮):n(硝态氮):n(酰胺态氮)=0:75:25〕处理组根中的多糖含量和叶中的叶绿素相对含量均最高,T3〔n(铵态氮):n(硝态氮):n(酰胺态氮)=50:50:0〕处理组叶和根中的可溶性蛋白质含量均较高.各施氮处理组叶中靛玉红含量总体上显著高于对照组,多数施氮处理组叶中靛蓝含量则显著低于对照组,但各施氮处理组的单株叶中靛蓝和靛玉红积累量总体上高于对照组;其中,T2〔n(铵态氮):n(硝态氮):n(酰胺态氮)=75:25:0〕处理组叶中靛玉红含量及其单株积累量均最高,T6处理组叶中靛蓝含量最高,而单株叶中靛蓝积累量则以T3处理组最高.各施氮处理组根中(R,S)-告依春含量总体上显著低于对照组,其中,以T1〔n(铵态氮):n(硝态氮):n(酰胺态氮)=100:0:0〕处理组根中(R,S)-告依春含量最高,T4处理组单株根中(R,S)-告依春积累量最高.综合分析结果表明:按不同配比施用不同形态氮素,夏播菘蓝的生长及活性成分含量有明显差异,因此,若以收获叶为目的,结合叶中靛玉红含量,建议施用铵态氮和硝态氮物质的量比为75:25的复合氮肥;若以收获根为目的,结合根中(R,S)-告依春含量,建议施用铵态氮和硝态氮物质的量比为25:75的复合氮肥.     

Nitrogen dynamics in the intact grasses Poa trivialis and Panicum maximum receiving contrasting supplies of nitrogen

The C(3) grass Poa trivialis and the C(4) grass Panicum maximum were grown in sand culture and received a complete nutrient solution with nitrogen supplied as 1.5 mol m(-3) NH(4)NO(3). (15)N tracer techniques were used to quantify the relative use of root uptake and mobilization in supplying nitrogen to growing leaves in intact plants which either continued to receive nitrogen or which received the complete nutrient solution without nitrogen. The allocation of both (15)N-labelled nitrogen uptake and unlabelled mobilized nitrogen indicated that, under their conditions of growth, the sink strength of growing leaves was relatively greater in P. maximum than P. trivialis. The supply of nitrogen by mobilization to side tillers of P. trivialis was completely stopped as the external nitrogen supply was reduced, whilst in P. maximum some allocation of mobilized nitrogen to side tillers, roots and growing leaves was maintained. In both plant species receiving an uninterrupted supply of nitrogen the allocation pattern of mobilized nitrogen differed from that of nitrogen derived from root uptake. Differences exist in the degree to which P. trivialis and P. maximum utilized uptake and mobilization to supply nitrogen to the growing leaves. In P. trivialis roots were always a net sink of mobilized nitrogen, irrespective of the external nitrogen supply. In P. maximum, roots were a net sink of mobilized nitrogen when external nitrogen was withdrawn, but exhibited both source and sink behaviour when nitrogen supply was continued.     

开放式空气CO2浓度增高对水稻N素吸收利用的影响

在大田栽培条件下 ,研究空气中CO2 浓度增高 (FACE) 2 0 0 μmol·mol-1对水稻N素吸收及其利用效率的影响 .结果表明 ,FACE处理使水稻不同生育时期的植株含N率显著下降 ;由于干物质生产量显著增大 ,FACE处理使水稻不同生育时期的N素累积量有所提高 ,但无显著影响 ;FACE处理能够显著提高移栽后 2 8d、抽穗期以及成熟期单位N素的干物质生产效率、单位N素的籽粒生产效率和显著提高水稻的N素收获指数 .高N处理的植株含N率、N素累积量均有所增加 ,但使N素生产效率呈现下降趋势 .     

Neonatal growth and survival of rodents in response to variation in maternal dietary nitrogen: life history strategy vs dietary niche

Nitrogen (N) enrichment of terrestrial ecosystems dramatically changes ecosystem diversity and structure of plant communities. Research designed to elucidate effects of nitrogen addition on mammalian assemblages is rare. We investigated nitrogen requirements of hispid cotton rats ( Sigmodon hispidus ) and fulvous harvest mice ( Reithrodontomys fulvescens ), small mammals native to the tallgrass prairie of the southern Great Plains, USA, to better understand population responses of these species to nitrogen enrichment. We studied reproductive requirements by measuring growth of offspring under varying levels of dietary nitrogen. We predicted that dietary niche would dictate nitrogen requirements, such that the larger herbivore ( S. hispidus ) would have a lower dietary need for nitrogen per unit mass than the small omnivore/granivore ( R. fulvescens ). Reproductive output (measured as mass gain of litters and offspring) was responsive to varying nitrogen in cotton rats but not in harvest mice. Nitrogen intake that supported 50% survival of juvenile harvest mice (1.34% dietary nitrogen) also was adequate for maximum growth (1.29%). Cotton rats potentially drew on maternal nutrient stores to support litter growth at low levels of dietary nitrogen (as low as 1.08%). Overall, nitrogen requirements for maximum reproduction were greater (2.31% dietary nitrogen) for cotton rats. We conclude that life history characteristics and body size constraints rather than dietary niche explain the differential species response to variation in dietary nitrogen. Our results imply that nitrogen enrichment in old-field succession in the southern Great Plains may lead to dominance by cotton rats and a reduction in diversity of the small-mammal assemblage. Consumers with similar abilities to take advantage of increased environmental nitrogen may likewise dominate other ecosystems.     

Some priority research areas in nitrogen studies

The more efficient management of the world's nitrogen resources, leading to greater plant productivity, depends on research which (a) improves the economic efficiency of nitrogen inputs into ecosystems, (b) makes more effective use of the nitrogen within the plant environment, and (c) reduces nitrogen losses, particularly from the root region. Ecosystem nitrogen budget studies can emphasize gaps in knowledge of nitrogen cycle processes.     

不同有机氮效率的甜菜基因型筛选及差异分析

通过对不同基因型甜菜土壤有机氮利用及吸收效率的筛选和差异分析,为土壤有机氮高效基因型甜菜的栽培及品种选育提供理论依据。2014-2015年选取100份不同基因型的甜菜材料通过室内及田间试验在甜菜的不同发育阶段比较并分析土壤有机氮效率,筛选出对有机氮利用及吸收效率均显著差异的高效和低效基因型甜菜材料。结果表明,初步筛选得到的有机氮高效基因型甜菜材料KWS8138、HI0466和有机氮低效基因型甜菜材料BETA176、T230苗期全株及根部有机氮利用效率（Organic Nitrogen Use Efficiency,ONUE）差异显著;之后通过田间试验对有机氮吸收效率（Organic Nitrogen Assimilation Efficiency,ONAE）做了进一步筛选,发现KWS8138不但对ONUE有显著优势,还具有较高的有机氮素吸收能力,包括苗期之后对土壤有机氮素的运转量较高,合理的根冠比等。有机氮低效基因型甜菜材料BETA176的有机氮素吸收利用能力很弱、氮素转运能力过低等限制了植株对有机氮素的合理利用,不利于有机氮效率的提高。因此确定KWS8138为有机氮高效基因型材料,BETA176为有机氮低效基因型材料,均可作为进一步试验的材料。有机氮高效基因型甜菜较高的土壤有机氮转运量及合理的根冠比促进了其对有机氮素的吸收,是有机氮高效的基础。较高的干物质生产效率反应了甜菜对有机氮素的高效利用,是有机氮高效的关键。     

施氮量对麦后直播棉氮素吸收利用的影响

以早熟棉中棉所50为材料进行麦后直播棉花试验,研究施氮量(0、60、120、150、180、240 kg N·hm^-2)对棉株氮素吸收、利用和分配的影响.结果表明： 增施氮肥提高了麦后直播棉不同生育阶段的氮吸收量,以盛花到见絮期的氮积累增量最大,并且改变了不同生育期间氮吸收比例,使棉花出苗到盛花期的氮吸收比例降低,盛花到吐絮期的氮吸收比例升高;增施氮肥还降低了生育后期中上部位果枝氮浓度的下降速率.麦后直播棉氮素和生物量累积以中下部果枝为主,在150～180 kg N·hm^-2施氮量下棉花产量、氮肥表观利用率、各果枝部位干物质和氮在生殖器官中的分配比例较高,氮浓度和氮累积量动态特征参数比较协调.高于180 kg N·hm^-2的施氮量导致棉花中部和下部果枝生殖器官生物量和氮素累积量、产量增幅和氮肥利用率降低,而低于150 kg N·hm^-2施氮量降低棉花整株干物质和氮经济系数,不利于高产形成.综合分析,150～180 kg N·hm^-2施氮量可作为长江流域下游棉区麦后直播棉的推荐施氮量.     

开放式空气CO2浓度增高对水稻N素吸收利用的影响

在大田栽培条件下,研究空气中CO₂浓度增高(FACE)200μmol·mol^-1对水稻N素吸收及其利用效率的影响.结果表明,FACE处理使水稻不同生育时期的植株含N率显著下降;由于干物质生产量显著增大,FACE处理使水稻不同生育时期的N素累积量有所提高,但无显著影响;FACE处理能够显著提高移栽后28d、抽穗期以及成熟期单位N素的干物质生产效率、单位N素的籽粒生产效率和显著提高水稻的N素收获指数.高N处理的植株含N率、N素累积量均有所增加,但使N素生产效率呈现下降趋势.     

QTL and QTL x environment effects on agronomic and nitrogen acquisition traits in rice

Agricultural environments deteriorate due to excess nitrogen application.Breeding for low nitrogen responsive genotypes can reduce soil nitrogen input.Rice genotypes respond variably to soil available nitrogen.The present study attempted quantification of genotype x nitrogen level interaction and mapping of quantitative trait loci (QTLs) associated with nitrogen use efficiency (NUE) and other associated agronomic traits.Twelve parameters were observed across a set of 82 double haploid (DH) lines derived from IR64/Azucena.Three nitrogen regimes namely,native (0 kg/ha; no nitrogen applied),optimum (100 kg/ha) and high (200 kg/ha) replicated thrice were the environments.The parents and DH lines were significantly varying for all traits under different nitrogen regimes.All traits except plant height recorded significant genotype x environment interaction.Individual plant yield was positively correlated with nitrogen use efficiency and nitrogen uptake.Sixteen QTLs were detected by composite interval mapping.Eleven QTLs showed significant QTL x environment interactions.On chromosome 3,seven QTLs were detected associated with nitrogen use,plant yield and associated traits.A QTL region between markers RZ678,RZ574 and RZ284 was associated with nitrogen use and yield.This chromosomal region was enriched with expressed gene sequences of known key nitrogen assimilation genes.     

氯嘧磺隆与尿素对土壤微生物生物量碳、氮及无机氮的影响

通过室内培养试验,研究了不同浓度氯嘧磺隆(20、200、2000 μg·kg-1土)单一施用及与尿素(120 mg· kg-1土)配合施用情况下,土壤微生物生物量碳、氮和土壤铵态氮、硝态氮随时间的动态变化规律.结果表明:各浓度氯嘧磺隆单独处理在整个培养期(60 d)中对微生物生物量碳、氮均有抑制作用,且浓度越高,后期抑制作用越强;各浓度氯嘧磺隆处理在培养前期对硝态氮、铵态氮没有明显影响,中期(15 d)能显著提高土壤中铵态氮的含量,后期(30 d后)显著提高了土壤中硝态氮的含量.尿素单独施用及与氯嘧磺隆配施均能在短时间内增加微生物生物量碳、氮,但随后配施处理的促进作用减弱;尿素单独和配施均能持久增加土壤中铵态氮、硝态氮含量.     

氮素减施对茶树光合作用和氮肥利用率的影响

为探讨当前茶园施肥水平下氮素减施对茶树的生长与氮肥利用率的影响,在大田条件下设置不施氮(空白对照CK),纯氮16 kg·667 m^-2(减氮55.6%,处理A)、纯氮26kg·667 m^-2(减氮27.8%,处理B)、纯氮36 kg·667 m^-2(常规施氮CF)4个施氮处理,研究氮素用量减施对茶树光合作用、产量、氮素吸收及其利用效率的影响。结果表明:与CF比较,处理B能提高茶树的叶绿素含量、净光合速率(Pn)、气孔导度(Gs)、蒸腾速率(Tr),降低胞间CO2浓度(Ci)和水分利用率(WUE);各施氮肥处理中以处理B的产量最高,氮肥农学效率最高,土壤养分剩余较少,氮素的利用效率较高;CF产量较处理B增加不显著,氮肥农学效率降低,土壤未利用养分较多。因此,减氮27.8%的施肥能保证与常规施肥的茶叶产量,氮素利用率明显提升,有利于茶业的可持续发展。     

川西亚高山不同海拔森林土壤活性氮库及净氮矿化的季节动态

研究了川西理县毕棚沟不同海拔梯度(3600 m、3300 m和3000 m)森林群落土壤活性氮库及土壤净氮矿化速率的季节动态.结果表明: 研究区森林土壤活性氮库(铵态氮、硝态氮、微生物生物量氮和可溶性有机氮)及净氮矿化速率存在明显的季节变化,但不同形态土壤活性氮库的季节动态有一定差异.4个采样时期(非生长季与生长季初期、中期及末期)各海拔土壤硝态氮浓度(8.38～89.60 mg·kg^-1)均显著高于铵态氮浓度(0.44～8.43 mg·kg^-1).生长季初期各海拔梯度的土壤净氮矿化速率均表现为负值(-0.77～-0.56 mg·kg^-1·d^-1),而非生长季、生长季中期和末期均为正值.除硝态氮外,不同海拔的土壤铵态氮、微生物生物量氮和可溶性有机氮浓度的差异极显著,海拔对它们的影响与季节变化有关.该区土壤净氮矿化以硝化为主,且氮矿化过程不受海拔梯度的影响.冬季土壤净氮矿化明显(0.42～099 mg·kg^-1·d^-1),早春高的土壤无机氮可能为植物生长提供基础养分,也可能通过淋溶方式从系统中丢失.     

Nitrogen Uptake and Plant Growth I. Effect of Nitrogen Removal on Growth of Polygonum cuspidatum

Polygonun cuspidatum was grown hydroponically to examine theeffect of nitrogen removal from the nutrient solution upon plantgrowth and the partitioning of dry matter and nitrogen amongorgans. Nitrogen removal reduced the growth rate mainly dueto the reduced growth of leaf area. Accelerated root growthwas observed only in plants which earlier had received highlevels of nitrogen. Nitrogen removal caused almost exclusiveallocation of available nitrogen to root growth. Nitrogen fluxfrom the shoot to the root occurred in plants which had receivedlow nitrogen. Not only was net assimilation rate (NAR) littleaffected by nitrogen removal, but it also was not correlatedwith the concentration of leaf nitrogen on an area basis. Light-saturatedCO₂ exchange rate (CER) was highly correlated with the concentrationof leaf nitrogen. Nitrogen use efficiency (NUE) in CER (CERdivided by leaf nitrogen) remained constant against leaf nitrogen,indicating efficient use of nitrogen under light saturation,while NUE in terms of NAR decreased with higher concentrationof leaf nitrogen. Polygonum cuspidatum Sieb. et Zuce., CO₂ exchange rate, growth analysis, leaf nitrogen, net assimilation rate, nitrogen use efficiency, partitioning of dry matter and nitrogen     

不同形态氮素施肥对小黑杨幼苗生长的影响

以小黑杨当年播种苗为材料,研究了不同施氮量（12,24和48 mg·株^-1）和不同形态氮素（有机氮和无机氮）施肥对小黑杨幼苗生长的影响,以探讨小黑杨对氮基酸类有机氮素施肥的生长响应。结果表明：无论施无机氮（硝酸铵）还是有机氮（精氨酸）,小黑杨幼苗的苗高、地径、总生物量都是随施氮量的增加而增加,但是中等施氮量处理的幼苗氮利用效率最高。无机氮（硝酸铵）和有机氮（精氨酸）处理的幼苗在相同施氮水平下生长表现无明显差异,施用有机氮可以与施用无机氮获得相同的促进苗木生长的效果。不同氨基酸及其组合肥料施用对小黑杨幼苗生长的影响显著。单一氨基酸施肥情况下,施用精氨酸促进苗木生长的效果最好,谷氨酸次之,甘氨酸最差;氨基酸组合施肥情况下,有精氨酸的组合施肥苗木生长好,有甘氨酸的组合施肥苗木生长差。不同氨基酸施肥处理对小黑杨幼苗各器官氮含量没有明显影响。     

Nitrogen source influences natural abundance (15)N of Escherichia coli.

Escherichia coli cells were forced to mineralize or assimilate nitrogen in vitro by manipulating substrate carbon and nitrogen availability. When grown on an organic nitrogen source, E. coli cells released NH(4)(+) and were enriched in (15)N relative to the nitrogen source (1.6-3.1 per thousand). However, when cells were grown on an inorganic nitrogen source, the biomass was depleted (6.1-9.1 per thousand) relative to the source. By measuring (15)N enrichment of microorganisms relative to nitrogen pools, ecosystem ecologists may be able to determine if microorganisms are assimilating or mineralizing nitrogen.     

不同施氮量对杂交酸模叶片光合电子流分配的影响

研究了不同施氮量对高蛋白含量植物杂交酸模(Rumex patientiaxR.tianschanicus)叶片中总光合电子流和分配在碳同化、光呼吸、Mehler反应以及氮代谢上的光合电子流的影响,并研究了不同施氮量对硝酸还原酶(NR)和谷氨酰胺合成酶(GS)的活性、叶片的蛋白质含量及叶绿素含量的影响。结果表明随着施氮量的增加,硝酸还原酶和谷氨酰胺合成酶的活性都显著提高,同时更多的光合电子流分配到氮代谢和光呼吸。氮代谢所需光合电子流约占总光合电子流的15%～21%。缺氮并没有造成光合电子流向Mehler反应分配的增加。     

植被不同退化状态下尕海湿地土壤氮含量及酶活性特征

土壤中氮素的吸收、转化及含量的变化是影响植被生长的关键因素。为探讨湿地植被不同退化状态对土壤氮组分含量和相关酶活性的影响,以及土壤氮组分含量与相关酶活性之间的关系,以甘南尕海湿地不同植被退化状态样地（未退化CK、轻度退化SD、中度退化MD和重度退化HD）为研究对象,采用野外采样与室内实验相结合的方法,分析了植被不同退化状态下不同形态氮组分（全氮、铵态氮、硝态氮和微生物量氮）含量的变化特征,以及土壤氮转化酶（蛋白酶、脲酶、硝酸还原酶和亚硝酸还原酶）活性之间的相关关系。结果表明：（1）在植被退化状态下,土壤含水量逐渐减小,土壤温度呈先减小后增大的趋势;（2）随着植被退化程度的加剧,硝态氮含量呈增加趋势,而全氮、铵态氮和微生物量氮含量均随退化程度加剧呈减小趋势;土壤蛋白酶活性随退化程度的加剧而减小,脲酶活性呈先减小后增大的趋势,重度退化活性最高,轻度退化最低;硝酸还原酶活性随退化程度的加剧而增加,亚硝酸还原酶活性表现为"升-降-升"的变化趋势,即轻度退化活性最高,未退化和中度退化较低;（3）土壤蛋白酶活性与全氮、铵态氮和微生物量氮呈极显著正相关关系（P < 0.01）,与硝态氮含量呈显著负相关关系（P < 0.05）;硝酸还原酶活性与蛋白酶活性恰好相反;脲酶活性与微生物量氮含量呈极显著正相关关系（P < 0.01）,与全氮含量呈显著正相关关系（P < 0.05）;亚硝酸还原酶活性与全氮和铵态氮含量呈极显著正相关关系（P < 0.01）,与硝态氮含量呈显著负相关关系（P < 0.05）。综上,在尕海湿地植被退化条件下,土壤氮组分含量增加可以有效提高相关酶活性。     

Effects of Different Nitrogen and Phosphorus Synergistic Fertilizer on Enzymes and Genes Related to Nitrogen Metabolism in Wheat

In recent years, in order to improve nutrient use efficiency, especially nitrogen use efficiency, fertilizer value-added technology has been developed rapidly. However, the mechanism of the effect of synergistic fertilizer on plant nitrogen utilization is not clear. A study was, therefore, conducted to explore the activities and gene expression of key enzymes for nitrogen assimilation and the gene expression of nitrogen transporters in wheat after the application of synergistic fertilizer. Soil column experiment was set up in Qingdao Agricultural University experimental base from October 2018 to June 2019. Maleic acid and itaconic acid were copolymerized with acrylic acid as cross-linking monomer to make a fluid gel, which was sprayed on the fertilizer surface to make nitrogen and phosphorus synergistic fertilizer. A total of 6 treatments was set according to different nitrogen and phosphorus fertilizer ratios: (1) 100% common nitrogen fertilizer+100% common phosphate fertilizer (2) 70% nitrogen synergistic fertilizer+100% phosphorus synergistic fertilizer; (3) 100% nitrogen synergistic fertilizer+70% phosphorus synergistic fertilizer; (4) 100% nitrogen synergistic fertilizer+100% phosphorus synergistic fertilizer; (5) 70% nitrogen synergistic fertilizer+70% phosphorus synergistic fertilizer; (6) 100% commercial nitrogen synergistic fertilizer+100% commercial phosphorus synergistic fertilizer. The results are as follows: (1) the enzyme activities of wheat plants under synergistic fertilizer condition were higher than those under ordinary fertilizer, except under the treatment that nitrogen and phosphorus synergistic fertilizer were both reduced; (2) the expression level of the genes under the treatment “100% nitrogen synergistic fertilizer+100% phosphorus synergistic fertilizer” was significantly higher than those in other treatments. Combined with the higher performance of nitrogen concentration in various parts of the plant under the condition of applying synergistic fertilizer, this study indicated that the application of synergistic fertilizer can improve the nitrogen metabolism of the plant by increasing the nitrogen level in the rhizosphere soil, inducing the expression of nitrogen transporter genes and key assimilation enzymes genes.