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1.
Summary Short-term absorption experiments were conducted with intact barley (Hordeum vulgare L.) seedlings to observe the effects of the osmotic potential (Ψπ) and salt species on nitrate uptake andin vivo nitrate reduction. The experiments consisted of growing barley seedlings for 5 days in complete nutrient solutions salinized
to (Ψπ) levels of −0.6, −1.8, −3.0, −4.2, and −5.4 bars with NaCl, CaCl2 or Na2SO4. After the absorption period, the seedlings were separated into shoots and roots, weighed, then analyzed for NO3. The nutrient solutions were sampled for NO3 analysis each day immediately before renewing the solutions. The accumulative loss of NO3 from the solutions was considered to be uptake whereas NO3 reduction was the difference between uptake and seedling content. Lowering the (Ψπ) of the nutrient solutions resulted in decreased concentrations of NO3 in the plant, little or no effect (except at the lowest (Ψπ) level) on uptake, and increased nitrate reductase activity. Increased rates of NO3 reduction were in particular associated with the Cl concentration of the nutrient solution. 相似文献
2.
Although the micronutrient boron (B) apparently exerts no influences on the induction of somatic embryogenesis of cultured
carrot petiole explants, strong influences on the development of somatic embryos were observed (0–8 mg l−1 B). At lower B concentrations the development of roots is promoted with simultaneous retardation of shoot development and
at higher B concentrations shoot development is favored at the expenses of the development of the root system. Parallel to
this, the ratios of concentrations of endogenous indole-3-acetic acid to total cytokinins (with one exception) changes continuously
from 4 (zero B) to 0.22 (8 mg l−1 B). Analogies to morphogenetic reactions following the application of various ratios of auxins/cytokinins to the nutrient
medium of cultured tissue (Skoog and Miller, 1957 and others) are suggested. An increase in the B concentration in the nutrient
medium results generally in a reduced concentration of endogenous abscisic acid. 相似文献
3.
The responses of leaf water parameters to drought were examined using three sunflower (Helianthus annuus L.) genotypes. Osmotic potential at full water saturation (π100), apoplastic water fraction (AWF) and bulk elastic modulus (BEM) were determined by pressure-volume curve analysis on well
watered or on water-stressed plants (−1.0 MPa Ψ1 < −1.5 MPa) previously drought-pretreated or not. The drought-pretreated plants were subjected to a 7-day drought period
(predawn leaf water potential reached −0.9 MPa) followed by 8 days of rewatering. In well watered plants, all genotypes in
response to drought acclimation displayed a significantly decreased π100 associated with a decrease in the leaf water potential at the turgor-loss point (decrease in Ψtlp was between 0.15 and 0.21 MPa, depending on the genotype). In two genotypes, drought acclimation affected the partitioning
of water between the apoplastic and symplastic fractions without any effect on the total amount of water in the leaves. As
a third genotype displayed no modification of AWF and BEM after drought acclimation, the decreased π100 was only due to the net accumulation of solutes and was consistent with the adjustment of the photochemical efficiency observed
previously in this genotype in response to drought acclimation. In water-stressed plants, the osmotic adjustment (OA) can
increase further beyond that observed in response to the drought pretreatment. However, the maintenance of photosynthetic
rate and stomatal conductance at low leaf water potentials not only depends on the extent of osmotic adjustment, but also
on the interaction between OA and AWF or BEM. Adaptative responses of leaf water parameters to drought are thus quite contrasted
in sunflower genotypes.
This revised version was published online in June 2006 with corrections to the Cover Date. 相似文献
4.
In a sand culture experiment we investigated the effects of boron (0.01, 0.19, 0.46 and 0.93 mol m−3 B, as H3BO3), sodium chloride (0, 100 and 200 mol m−3 NaCl) and combined B and NaCl, over 36 days, on growth, water use and foliar ion concentrations of nine week-old seedlings
of three fast-growing, commercial eucalypts ( Eucalyptus camaldulensis Dehnh. , E. globulus Labill. ssp. globulus and E. grandis
W.Hill.). Shoot dry weight was significantly reduced by high concentrations of NaCl (p < 0.001) and by B and NaCl in combination
(p ≤ 0.05) but not by B alone. Root dry weight was significantly reduced by both NaCl (p < 0.001) and B (p < 0.001), but not
by combined B and NaCl. Foliar B concentrations increased with higher concentrations of applied B and decreased with higher
NaCl concentrations. Foliar Na concentrations were greater with higher NaCl concentrations, whereas B application had no significant
effect on foliar Na concentrations. All three species accumulated relatively high B concentrations in leaves. Severe boron
toxicity symptoms (BTS) were apparent only when leaf B concentrations exceeded 50 mol x 10−6 g−1, but even at these high concentrations plant growth was only slightly reduced. E. camaldulensis showed least development
of BTS, the lowest leaf B concentrations and least reduction in height growth due to B and NaCl. The results suggest that
there was a correlation between both B tolerance and B accumulation in leaves and between tolerance to B and NaCl.
This revised version was published online in June 2006 with corrections to the Cover Date. 相似文献
5.
Water potential and sap flow rate in adult trees with moist and dry soil as used for the assessment of root system depth 总被引:4,自引:0,他引:4
Sap flow rate (Qw) and leaf water potential (Ψw.leaf) in adult specimens of birch (Betula) and oak (Quercus) were measured under contrasting soil moisture conditions (Ψw.sofl). With sufficient soil moisture Qw reached about 250 cm3h−1 calculated per unit tree-trunk segment as given by 1 cm length of its circumference. In soil water-stress conditions (when
Ψw.leaf = = −15 × 105Pa), birch stopped transpiration and wilted. Oak transpired even when Ψw.leaf fell below −20 × 105Pa. The relation between Qw and Ψw.leaf was always linear and with various Ψw.soil differed in the slopes of regression lines only. Hydraulic conductance (Kwcu) with nonlimiting moisture conditions reached about 6 × 10−9m3 10−5Pa−1s−1 and “conductivity” (“kwa”) when calculated per leaf area unit reached about 23 m 10−5Pa−1s−1. Kwcu and “kwa” were of about one half to nine times greater in birch than in oak. On the basis of relations between Ψw.soil at various depths, Ψw.leaf and Qw (resp. Kw) it is possible to assess the maximal rooting depth and the effective depth where the maximum of absorption of roots occurs.
It is to be seen that the root system macrostructure substantially participates in the drought avoidance of adult trees in
a forest stand. 相似文献
6.
This study examined whether ‘Risnod2’ and ‘Risnod27’ non-nodulating mutants of pea (Pisum sativum L.) provided with increasing concentrations of nitrate could achieve a growth and nitrogen accumulation comparable to their
parental N2-fixing cv. Finale. In the cv. Finale, nodule number, nodule dry mass accumulation, total C2H2-reducing activity of nodulated roots (TAR) and estimated N2 fixation were considerably inhibited at 5.0 and 10.0 mM root medium NO3
− concentrations. In contrast a 0.63 mM level stimulated both the nodule dry mass and TAR. The cv. Finale N2-fixing plants grown on 0 to 2.5 mM NO3
− levels had higher shoot N concentrations than the Nod− mutants, but within the 5.0 to 10.0 mM levels the Nod− mutants approached or even overtopped the N concentration of the cv. Finale plants. Compared with a high positive correlation
found in the Nod− mutants, shoot N concentration in the cv. Finale was negatively correlated with the root medium NO3
− concentration. The pattern of nitrogen content in shoot dry mass was very similar to that seen in the shoot dry mass accumulation.
The Nod− mutants grown on the 5.0 and/or 10.0 mM NO3
− level had plant dry mass, shoot nitrogen concentration, shoot nitrogen content, and root/shoot dry mass ratio comparable
with those of the nodulating cv. Finale grown on the same nitrate levels. 相似文献
7.
Seasonal leaf water relations characteristics were studied in fully irrigated spring barley (Hordeum distichum L. cv. Gunnar) fertilized at low (50 kg K ha−1) or high (200 kg K ha−1) levels of potassium applied as KCl. The investigation was undertaken from about 14 days before anthesis until the milk ripe
stage in leaves of different position and age. Additionally, the effects of severe water stress on leaf water relations were
studied in the middle of the grain filling period in spring barley (cv. Alis). The leaf water relations characteristics were
determined by the pressure volume (PV) technique.
Water relations of fully irrigated plants were compared in leaf No 7 with the water relations of slowly droughted plants (cv.
Alis). Leaf osmotic potential at full turgor (ψ
π
100
) decreased 0.1 to 0.3 MPa in droughted leaves indicating a limited osmotic adjustment due to solute accumulation. The leaf
osmotic potential at zero turgor (ψ
π
0
) was about −2.2 MPa in fully irrigated plants and −2.6 MPa in droughted plants. The relative water content at zero turgor
(R0) decreased 0.1 unit in severely droughted leaves. The ratio of turgid leaf weight to dry weight (TW/DW) tended to be increased
by drought. The tissue modulus of elasticity (ε) decreased in droughted plants and together with osmotic adjustment mediated
turgor maintenance during drought. A similar response to drought was found in low and high K plants except that the R0 and ε values tended to be higher in the high K plants. Conclusively, during drought limited osmotic adjustment and increase
in elasticity of the leaf tissue mediated turgor maintenance. These effects were only slightly modified by high potassium
application.
The seasonal analysis in fully irrigated plants (cv. Gunnar) showed that within about 14 days from leaf emergence ψ
π
100
decreased from about −0.9 to −1.6 MPa in leaf No 7 (counting the first leaf to emerge as number one) and from about −1.1
to −1.9 MPa in leaf No 8 (the flag leaf) due to solute accumulation. A similar decrease took place in ψ
π
0
except that the level of ψ
π
0
was displaced to a lower level of about 0.2 to 0.3 MPa. Both ψ
π
100
and ψ
π
0
tended to be 0.05 to 0.10 MPa lower in high K than in low K plants. R0 was about 0.8 to 0.9 and was independent of leaf position and age, but tended to be highest in high K plants. The TW/DW ratio
decreased from about 5.5 in leaf No 6 to 4.5 in leaf No 7 and 3.8 in leaf No 8. The TW/DW ratio was 4 to 10% higher in high
K than in low K plants indicating larger leaf cell size in the former. The apoplastic water content (Va) at full turgor constituted about 15% in leaf No 7. ε was maximum at full turgor and varied from about 11 to 34 MPa. ε tended
to be higher in high K plants. Conclusively, in fully watered plants an ontogenetically determined accumulation of solutes
(probably organic as discussed) occurred in the leaves independent of K application. The main effect of high K application
on water relations was an increase in leaf water content and a slight decrease in leaf ψπ.
The effect of K status on growth and drought resistance is discussed. 相似文献
8.
We analysed plant growth, ion accumulation, leaf water relations, and gas exchange of Avicennia germinans (L.) L. subjected to a long-term, controlled salinity gradient from 0 to 55 ‰. Growth and leaf area were affected by salinity
higher than 10 ‰. As salinity increased, the predawn leaf water potential (Ψw) and leaf osmotic potential (Ψs) decreased. Leaf Ψw was at least −0.32 MPa lower than the Ψw of solution. Na+ and K+ ions explained about 78 % of decrease in Ψs. K+ tissue water concentration decreased by more than 60 % in all salinity treatments as compared with those grown at 0 ‰. Inversely,
Na+ concentration in tissue water increased with nutrient solution salinity. The maximum net photosynthetic rate (P
N) and stomatal conductance (g
s) decreased by 68 and 82 %, respectively, as salinity increased from 0 to 55 ‰; the intercellular CO2 concentration (C
i) followed the same trend. The P
N as a function of C
i showed that both the initial linear slope and upper plateau of the P
N
vs. C
i curve were markedly affected by high salinity (40 and 55 ‰). 相似文献
9.
Jian Wu Henk Schat Rifei Sun Maarten Koornneef Xiaowu Wang Mark G. M. Aarts 《Plant and Soil》2007,291(1-2):167-180
Brassica rapa L. is an important vegetable crop in eastern Asia. The objective of this study was to investigate the genetic variation in
leaf Zn, Fe and Mn accumulation, Zn toxicity tolerance and Zn efficiency in B. rapa. In total 188 accessions were screened for their Zn-related characteristics in hydroponic culture. In experiment 1, mineral
assays on 111 accessions grown under sufficient Zn supply (2 μM ZnSO4) revealed a variation range of 23.2–155.9 μg g−1 dry weight (d. wt.) for Zn, 60.3–350.1 μg g−1 d. wt. for Fe and 20.9–53.3 μg g−1 d. wt. for the Mn concentration in shoot. The investigation of tolerance to excessive Zn (800 μM ZnSO4) on 158 accessions, by using visual toxicity symptom parameters (TSPs), identified different levels of tolerance in B. rapa. In experiment 2, a selected sub-set of accessions from experiment 1 was characterized in more detail for their mineral accumulation
and tolerance to excessive Zn supply (100 μM and 300 μM ZnSO4). In this experiment Zn tolerance (ZT) determined by relative root or shoot dry biomass varied about 2-fold. The same six
accessions were also examined for Zn efficiency, determined as relative growth under 0 μM ZnSO4 compared to 2 μM ZnSO4. Zn efficiency varied 1.8-fold based on shoot dry biomass and 2.6-fold variation based on root dry biomass. Zn accumulation
was strongly correlated with Mn and Fe accumulation both under sufficient and deficient Zn supply. In conclusion, there is
substantial variation for Zn accumulation, Zn toxicity tolerance and Zn efficiency in Brassica rapa L., which would allow selective breeding for these traits. 相似文献
10.
Chun-Wang Xiao Osbert J. Sun Guang-Sheng Zhou Jing-Zhu Zhao Gang Wu 《Trees - Structure and Function》2005,19(6):712-721
We studied the responses of leaf water potential (Ψw), morphology, biomass accumulation and allocation, and canopy productivity index (CPI) to the combined effects of elevated
CO2 and drought stress in Caragana intermedia seedlings. Seedlings were grown at two CO2 concentrations (350 and 700 μmol mol−1) interacted with three water regimes (60–70%, 45–55%, and 30–40% of field capacity of soil). Elevated CO2 significantly increased Ψw, decreased specific leaf area (SLA) and leaf area ratio (LAR) of drought-stressed seedlings, and increased tree height, basal
diameter, shoot biomass, root biomass as well as total biomass under the all the three water regimes. Growth responses to
elevated CO2 were greater in well-watered seedlings than in drought-stressed seedlings. CPI was significantly increased by elevated CO2, and the increase in CPI became stronger as the level of drought stress increased. There were significant interactions between
elevated CO2 and drought stress on leaf water potential, basal diameter, leaf area, and biomass accumulation. Our results suggest that
elevated CO2 may enhance drought avoidance and improved water relations, thus weakening the effect of drought stress on growth of C. intermedia seedings. 相似文献
11.
In sunflower (Helianthus annuus L.) grown under controlled conditions and subjected to drought by withholding watering, net photosynthetic rate (P
N) and stomatal conductance (g
s) of attached leaves decreased as leaf water potential (Ψw) declined from −0.3 to −2.9 MPa. Although g
s decreased over the whole range of Ψw, nearly constant values in the intercellular CO2 concentrations (C
i) were observed as Ψw decreased to −1.8 MPa, but C
i increased as Ψw decreased further. Relative quantum yield, photochemical quenching, and the apparent quantum yield of photosynthesis decreased
with water deficit, whereas non-photochemical quenching (qNP) increased progressively. A highly significant negative relationship between qNP and ATP content was observed. Water deficit did not alter the pyridine nucleotide concentration but decreased ATP content
suggesting metabolic impairment. At a photon flux density of 550 μmol m−2 s−1, the allocation of electrons from photosystem (PS) 2 to O2 reduction was increased by 51 %, while the allocation to CO2 assimilation was diminished by 32 %, as Ψw declined from −0.3 to −2.9 MPa. A significant linear relationship between mean P
N and the rate of total linear electron transport was observed in well watered plants, the correlation becoming curvilinear
when water deficit increased. The maximum quantum yield of PS2 was not affected by water deficit, whereas qP declined only at very severe stress and the excess photon energy was dissipated by increasing qNP indicating that a greater proportion of the energy was thermally dissipated. This accounted for the apparent down-regulation
of PS2 and supported the protective role of qNP against photoinhibition in sunflower. 相似文献
12.
Y. H. Qin Jaime A. Teixeira da Silva J. H. Bi S. L. Zhang G. B. Hu 《Plant Growth Regulation》2011,65(1):183-193
By identifying antibiotics that had the least phytotoxic effects on explants during genetic transformation, we evaluated the
effect of various antibiotics on callus induction and morphogenesis from leaf explants and in vitro growth of Fragaria × ananassa Duch. cv. Toyonaka. Results showed that kanamycin (Kan) significantly inhibited callus induction, bud differentiation and
root morphogenesis while carbenicillin (Carb), cefotaxime (Cef) and an equal concentration of Cef and Carb up to 500 mg L−1 had no significant effects on callus induction and shoot growth. Kan, even at 2.5 mg L−1, significantly inhibited callus induction, shoot regeneration and root formation, while no shoots regenerated at concentrations
above 15 mg L−1. Rooting was completely inhibited in the presence of 50 mg L−1 Kan. Cef had negative effects on shoot regeneration from leaf explants and in vitro growth of strawberry. Compared to Cef,
Carb at ≤300 mg L−1 significantly promoted shoot and root organogenesis. However, an equal concentration of Carb plus Cef could alleviate the
negative effect of Cef on strawberry. Results from relative electrolyte leakage, root and antioxidant activities, O2·− production rate, H2O2, proline and MDA contents showed that Kan, Cef and Carb caused electrolyte leakage and triggered active enzymatic processes
and metabolism. This offers a possible mechanism for the inhibition or stimulation of strawberry growth caused by these antibiotics. 相似文献
13.
In a previous study on the effects of N-supply on leaf cell elongation, the spatial distribution of relative cell elongation
rates (RCER), epidermal cell turgor, osmotic pressure (OP) and water potential (Ψ) along the elongation zone of the third
leaf of barley was determined (W. Fricke et al. 1997, Planta 202: 522–530). The results suggested that in plants receiving
N at fixed relative addition rates (N-supply limitation of growth), cell elongation was rate-limited by the rate of solute
provision, whereas in plants growing on complete nutrient solution containing excessive amounts of N (N-demand limitation),
cell elongation was rate-limited by the rate of water supply or wall yielding. In the present paper, these suggestions were
tested further. The generation rates of cell OP, turgor and Ψ along the elongation zone were calculated by applying the continuity
equation of fluid dynamics to the previous data. To allow a more conclusive interpretation of results, anatomical data were
collected and bulk solute concentrations determined. The rate of OP generation generally exceeded the rate of turgor generation.
As a result, negative values of cell Ψ were created, particularly in demand-limited plants. These plants showed highest RCER
along the elongation zone and a Ψ gradient of at least −0.15 MPa between water source (xylem) and expanding epidermal cells.
The latter was similar to a theoretically predicted value (−0.18 MPa). Highest rates of OP generation were observed in demand-limited
plants, with a maximum rate of 0.112 MPa · h−1 at 16–20 mm from the leaf base. This was almost twice the rate in N-supply-limited plants and implied that the cells in the
leaf elongation zone were capable of importing (or synthesising) every minute almost 1 mM of osmolytes. Potassium, Cl− and NO3
− were the main inorganic osmolytes (only determined for demand-limited plants). Their concentrations suggest that, unlike
the situation in fully expanded epidermal cells, sugars are used to generate OP and turgor. Anatomical data revealed that
the zone of lateral cell expansion extended distally beyond the zone of cell elongation. It is concluded that leaf cell expansion
in barley relies on high rates of water and solute supply, rates that may not be sustainable during periods of sufficient
N-supply (limitation by water supply: Ψ gradients) or limiting N-supply (limitation by solute provision: reduced OP-generation
rates). To minimise the possibility of growth limitation by water and osmolyte provision, longitudinal and lateral cell expansion
peak at different locations along the growth zone.
Received: 15 October 1997 / Accepted: 12 March 1998 相似文献
14.
Genotypes of mustard (B. juncea) were evaluated for concurrent changes in leaf water potential (Ψ), leaf osmotic potential
(π), leaf turgor potential (P) and leaf relative water content (RWC) during moisture stress at reproductive stage of growth.
The slope ‘b’ in the regression between Ψ and π varied from 0.43 to 0.97 and was positively correlated with P and RWC. The
genotypes with ‘b’ around 0.7 were able to maintain P of about 0.5 MPa at Ψ of − 2.5 MPa and thus such value of ‘b’ seems
to provide enough degree of tolerance against drought. 相似文献
15.
The effects of liming on concentrations of boron and other elements in Norway spruce [Picea abies (L) Karst.] needles and in the mor humus layer were studied in long-term field experiments with and without B fertilizer on podzolic soils in Finland. Liming (2000+4000 kg ha-1 last applied 12 years before sampling) decreased needle B concentrations in the four youngest needle age classes from 6–10 mg kg-1 to 5 mg kg-1. In boron fertilized plots the corresponding concentrations were 23–35 mg kg-1 in control plots and 21–29 mg kg-1 in limed plots. Both liming and B fertilizer decreased the Mn concentrations of needles. In the humus layer, total B concentration was increased by both lime and B fertilizer, and Ca and Mg concentrations and pH were still considerably higher in the limed plots than controls. Liming decreased the organic matter concentration in humus layer, whilst B fertilizer increased it.The results about B uptake were confirmed in a pot experiment, in which additionally the roles of increased soil pH and increased soil Ca concentration were separated by means of comparing the effects of CaCO3 and CaSO4. Two-year-old bare-rooted Norway spruce seedlings were grown in mor humus during the extension growth of the new shoot. The two doses of lime increased the pH of soil from 4.1 to 5.6 to 6.1, and correspondingly decreased the B concentrations in new needles from 22 to 12 to 9 mg kg-1. However, CaSO4 did not affect the pH of the soil or needle B concentrations. Hence the liming effect on boron availability in these soils appeared to be caused by the increased pH rather than increased calcium concentration. 相似文献
16.
Alleviation of Ultraviolet-B Radiation-Induced Growth Inhibition of Green Gram by Triadimefon 总被引:1,自引:0,他引:1
Supplementary UV-B (12.2 kJ m−2 d−1 UV-BBE) provided to Vigna radiata for 2 h d−1 suppressed the length of root, shoot and whole plants, number of leaves, total leaf area, leaf area index, specific leaf
mass, fresh and dry mass of leaves and shoot, relative growth rate and net productivity. In unstressed green gram plants (10
kJ m−2 d−1 UV-BBE), triadimefon (TRIAD) (20 mg dm−3) enhanced growth in all parameters over control. The growth promoting effect of TRIAD enabled the UV-B impacted plants to
overcome the growth inhibitions to varying degrees indicating its protective potential against UV-B stress.
This revised version was published online in July 2006 with corrections to the Cover Date. 相似文献
17.
Application of computer assisted tomography to gamma and X-ray attenuation measurements and Na+-LIX microelectrodes were used to determine the spatial distributions of soil water content and Na+ concentrations respectively near single roots of eighteen day old lupin and radish plants. These quantities were monitored
at root depths of 3, 6 and 9 cm and at zero, 2, 4, 6, and 8 hour intervals from the diurnal commencement of transpiration.
The plants were subjected to two levels of transpirational demand and five Na+ soil solution concentration levels. Water extraction rates for the lupin and radish roots increased continuously with time
but were substantially reduced with increasing Na+ concentration in the treatment. Water uptake was uniform along the length of the essentially constant diameter lupin roots
but decreased along the tapering radish roots as the diameter and hence the surface area per unit length of the roots decreased.
The accumulation of Na+ at the root surfaces of both plants increased gradually with time in a near linear fashion and was slightly higher under
the higher transpiration demand. These increases were not exponential as would be expected with non-absorption by the roots
and this is considered to be due to back diffusion at the relatively high water contents used. At these water contents matric
potentials had a much smaller influence on transpiration than osmotic potentials. The relationships between leaf water potentials
(Ψ1) and osmotic potentials at the root surfaces were linear with the decreases in Ψ1 almost exactly reflecting the decreases in Ψπ indicating rapid plant adjustment. Leaf water potentials decreased progressively with time and the relationships between
leaf water potential and the transpiration rate were also linear supporting the suggestion of constant plant resistances at
any given concentration. 相似文献
18.
Variation in phosphorus efficiency among 73 bread and durum wheat genotypes grown in a phosphorus-deficient calcareous soil 总被引:3,自引:0,他引:3
A greenhouse experiment was carried out to study the severity of phosphorus (P) deficiency symptoms on leaves, shoot dry matter
production, and shoot concentration and content (the total amount per shoot) of P in 39 bread wheat (Triticum aestivum L.) and 34 durum wheat (Triticum durum L.) genotypes grown in a severely P-deficient calcareous soil with low (20mgPkg−1 soil) and adequate (80mgPkg−1 soil) P supply for 39 days. As the seed P concentration or content can affect plant performance under P-deficient conditions,
the seeds of the genotypes used in the present study were also analyzed for P concentration. Phosphorus efficiency (relative
shoot growth) of genotypes, calculated by the ratio of shoot dry matter production under low P to that under adequate P supply,
significantly differed among the genotypes, and varied between 46.7% and 78.6%. Phosphorus efficiency ranged from 51% to 71%
with an average of 61% for bread and from 47% to 79% with an average of 66% for durum wheat genotypes. There was no correlation
between P efficiency ratio and P concentration of plants (R
2=0.0001), but P efficiency of all bread and durum wheat genotypes showed a very significant correlation with the P content
(the total amount of P per shoot) (R
2=0.333***). The relationship between the P efficiency and total amount of P per shoot was much more significant in bread (R
2=0.341***) than in durum wheat (R
2=0.135*). Like shoot P concentrations, also severity of visible leaf symptoms of P deficiency on older leaves, including leaf chlorosis
and necrosis, did not correlate with P efficiency. In most cases, genotypes showing higher P efficiency had higher absolute
shoot dry weight under P deficient conditions. Under P deficient conditions, the absolute shoot dry weight very significantly
correlated with shoot P content (R
2=0.665***), but the correlation between the absolute shoot dry weight and shoot P concentration tended to be negative. There was also
variation in native seed P reserve of the genotypes, but this variation had no influence on the P efficiency. The results
indicate that the total amount of P per shoot and shoot dry matter production at low P supply are most reliable parameters
in ranking genotypes for P efficiency at early growth stage. In wheat germplasm tested in the present study, several wheat
genotypes are available showing both very high P efficiency and very high shoot content and concentration of P suggesting
that P acquisition ability should be most important mechanism for high P efficiency in such genotypes. On the other hand,
there are also genotypes in the germplasm having more or less same P concentration or P content in shoot but differing substantially
in P efficiency, indicating importance of P utilization at cellular level in P efficiency. All these results suggest that
P efficiency mechanisms can be different from one genotype to other within a given plant species. 相似文献
19.
The permeability of biological membranes to boric acid was investigated using the giant internodal cells of the charophyte
alga Chara corallina (Klein ex Will. Esk. R.D. Wood). The advantage of this system is that it is possible to distinguish between membrane transport
of boron (B) and complexing of B by plant cell walls. Influx of B was found to be rapid, with equilibrium between the intracellular
and extracellular phases being established after approximately 24 h when the external concentration was 50 μM. The intracellular
concentration at equilibrium was 55 μM, which is consistent with passive distribution of B across the membrane along with
a small amount of internal complexation. Efflux of B occurred with a similar half-time to influx, approximately 3 h, which
indicates that the intracellular B was not tightly complexed. The concentration dependence of short-term influx measured with
10B-enriched boric acid was biphasic. This was tentatively attributed to the operation of two separate transport systems, a
facilitated system that saturates at 5 μM, and a linear component due to simple diffusion of B through the membrane. V
max and K
m for the facilitated transport system were 135 pmol m−2 s−1 and 2 μM, respectively. The permeability coefficient for boric acid in the Chara plasmalemma estimated from the slope of the linear influx component was 4.4 × 10−7 cm s−1 which is an order of magnitude lower than computed from the ether:water partition coefficient for B.
Received: 14 August 2000 / Accepted: 16 September 2000 相似文献
20.
The effect of phosphorus (P) concentration in barley seed on seedling growth has not been much investigated. Consequently,
two experiments were conducted in the greenhouse to determine the effect of P concentration in barley seed (Hordeum vulgare L., cv. Empress) on the seedlings grown in sand-filled boxes receiving a culture solution without P. Seeds were selected
with three P concentrations: high-P (113.0 mmol P kg−1), medium-P (80.7 mmol P kg−1) and low-P (54.9 mmol P kg−1). At 21 days after sowing, the shoot and root yield or shoot height was the least with seedlings from low-P seed. In the
other experiment, high-P and low-P seeds were wetted with distilled water or with a solution of 25.8 cmol L−1 of NaH2PO4 for 24 h, and then grown for 31 days. Solution P had been imbibed by seeds whether low or high in native P, but only the
imbibed P held by low native P seed benefited seedling dry matter accumulation and shoot elongation. The lack of benefit from
seed-imbibed P on seedlings grown from high-P barley seed was associated with low recovery of the imbibed P in those seedlings. 相似文献