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1.
We examined the expression of the KCC2 isoform of the K‐Cl cotransporter in the developing and adult brain, using an affinity‐purified antibody directed against a unique region of the KCC2 protein. Expression was shown to be limited to neurons at the cell bodies and cell processes in the hippocampus and cerebellum. Expression seemed to be the highest at the end of processes that originated from the CA1 pyramidal cells. Developmental up‐regulation of KCC2 expression was demonstrated in the entire rat brain by Northern and Western blot analyses, and in the hippocampus by immunofluorescence. Level of KCC2 expression was minimal at birth and increased significantly during postnatal development. This pattern of expression was opposite to the one of the Na‐K‐2Cl cotransporter that is highly expressed in immature brain and decreases during development. The up‐regulation of the K‐Cl cotransporter expression is consistent with the developmental down‐regulation of the intracellular Cl− concentration in neurons. The level of intracellular Cl−, in turn, determines the excitatory versus inhibitory response of the neurotransmitter γ‐aminobutyric acid in the immature versus mature brain. Finally, KCC2 expression was shown in dorsal root ganglion neurons, demonstrating that expression of the cotransporter is not strictly confined to central nervous system neurons. © 1999 John Wiley & Sons, Inc. J Neurobiol 39: 558–568, 1999 相似文献
2.
Courtney R. Sullivan Adam J. Funk Dan Shan Vahram Haroutunian Robert E. McCullumsmith 《PloS one》2015,10(3)
Alterations in GABAergic neurotransmission are implicated in several psychiatric illnesses, including schizophrenia. The Na-K-Cl and K-Cl cotransporters regulate intracellular chloride levels. Abnormalities in cotransporter expression levels could shift the chloride electrochemical gradient and impair GABAergic transmission. In this study, we performed Western blot analysis to investigate whether the Na-K-Cl and K-Cl cotransporter protein is abnormally expressed in the dorsal lateral prefrontal cortex and the anterior cingulate cortex in patients with schizophrenia versus a control group. We found decreased K-Cl cotransporter protein expression in the dorsal lateral prefrontal cortex, but not the anterior cingulate cortex, in subjects with schizophrenia, supporting the hypothesis of region level abnormal GABAergic function in the pathophysiology of schizophrenia. Subjects with schizophrenia off antipsychotic medication at the time of death had decreased K-Cl cotransporter protein expression compared to both normal controls and subjects with schizophrenia on antipsychotics. Our results provide evidence for KCC2 protein abnormalities in schizophrenia and suggest that antipsychotic medications might reverse deficits of this protein in the illness. 相似文献
3.
Activity-induced modification of GABAergic transmission contributes to the plasticity of neural circuits. In the present work we found that prolonged postsynaptic spiking of hippocampal neurons led to a shift in the reversal potential of GABA-induced Cl- currents (E(Cl)) toward positive levels in a duration- and frequency-dependent manner. This effect was abolished by blocking cytosolic Ca2+ elevation and mimicked by releasing Ca2+ from internal stores. Activity- and Ca2+-induced E(Cl) shifts were larger in mature neurons, which express the K-Cl cotransporter KCC2 at high levels, and inhibition of KCC2 occluded the shifts. Overexpression of KCC2 in young cultured neurons, which express lower levels of KCC2 and have a more positive E(Cl), resulted in hyperpolarized E(Cl) similar to that of mature cells. Importantly, these young KCC2-expressing neurons became responsive to neuronal spiking and Ca2+ elevation by showing positive E(Cl) shifts. Thus, repetitive postsynaptic spiking reduces the inhibitory action of GABA through a Ca2+-dependent downregulation of KCC2 function. 相似文献
4.
Uvarov P Ludwig A Markkanen M Pruunsild P Kaila K Delpire E Timmusk T Rivera C Airaksinen MS 《The Journal of biological chemistry》2007,282(42):30570-30576
The neuronal K-Cl cotransporter KCC2 maintains the low intracellular chloride concentration required for the hyperpolarizing actions of inhibitory neurotransmitters gamma-aminobutyric acid and glycine in the central nervous system. This study shows that the mammalian KCC2 gene (alias Slc12a5) generates two neuron-specific isoforms by using alternative promoters and first exons. The novel KCC2a isoform differs from the only previously known KCC2 isoform (now termed KCC2b) by 40 unique N-terminal amino acid residues, including a putative Ste20-related proline alanine-rich kinase-binding site. Ribonuclease protection and quantitative PCR assays indicated that KCC2a contributes 20-50% of total KCC2 mRNA expression in the neonatal mouse brain stem and spinal cord. In contrast to the marked increase in KCC2b mRNA levels in the cortex during postnatal development, the overall expression of KCC2a remains relatively constant and makes up only 5-10% of total KCC2 mRNA in the mature cortex. A rubidium uptake assay in human embryonic kidney 293 cells showed that the KCC2a isoform mediates furosemide-sensitive ion transport activity comparable with that of KCC2b. Mice that lack both KCC2 isoforms die at birth due to severe motor defects, including disrupted respiratory rhythm, whereas mice with a targeted disruption of the first exon of KCC2b survive for up to 2 weeks but eventually die due to spontaneous seizures. We show that these mice lack KCC2b but retain KCC2a mRNA. Thus, distinct populations of neurons show a differential dependence on the expression of the two isoforms: KCC2a expression in the absence of KCC2b is presumably sufficient to support vital neuronal functions in the brain stem and spinal cord but not in the cortex. 相似文献
5.
The neuron-specific K-Cl cotransporter, KCC2. Antibody development and initial characterization of the protein 总被引:6,自引:0,他引:6
Williams JR Sharp JW Kumari VG Wilson M Payne JA 《The Journal of biological chemistry》1999,274(18):12656-12664
The neuron-specific K-Cl cotransporter (KCC2) is hypothesized to function as an active Cl- extrusion pathway important in postsynaptic inhibition mediated by ligand-gated anion channels, like gamma-aminobutyric acid type A (GABAA) and glycine receptors. To understand better the functional role of KCC2 in the nervous system, we developed polyclonal antibodies to a KCC2 fusion protein and used these antibodies to characterize and localize KCC2 in the rat cerebellum. The antibodies specifically recognized the KCC2 protein which is an approximately 140-kDa glycoprotein detectable only within the central nervous system. The KCC2 protein displayed a robust and punctate distribution in primary cultured retinal amacrine cells known to form exclusively GABAAergic synapses in culture. In immunolocalization studies, KCC2 was absent from axons and glia but was highly expressed at neuronal somata and dendrites, indicating a specific postsynaptic distribution of the protein. In the granule cell layer, KCC2 exhibited a distinct colocalization with the beta2/beta3-subunits of the GABAA receptor at the plasma membrane of granule cell somata and at cerebellar glomeruli. KCC2 lightly labeled the plasma membrane of Purkinje cell somata. Within the molecular layer, KCC2 exhibited a distinctly punctate distribution along dendrites, indicating it may be highly localized at inhibitory synapses along these processes. The distinct postsynaptic localization of KCC2 and its colocalization with GABAA receptor in the cerebellum are consistent with the putative role of KCC2 in neuronal Cl- extrusion and postsynaptic inhibition. 相似文献
6.
An inhibitory neurotransmitter in mature brain, γ-aminobutyric acid (GABA) also appears to be excitatory early in development. The mechanisms underlying this shift are not well understood. In vitro studies have suggested that Na-K-Cl cotransport may have a role in modulating immature neuronal and oligodendrocyte responses to the neurotransmitter GABA. An in vivo developmental study would test this view. Therefore, we examined the expression of the BSC2 isoform of the Na-K-2Cl cotransporter in the postnatal developing rat brain. A comparison of sections from developing rat brains by in situ hybridization revealed a well-delineated temporal and spatial pattern of first increasing and then diminishing cotransporter expression. Na-K-2Cl mRNA expression in the cerebral cortex and hippocampus was highest in the first week of postnatal life and then diminished from postnatal day (PND) 14 to adult. Cotransporter signal in white-matter tracts of the cerebrum, cerebellum, peaked at PND 14. Expression was detected in cerebellar progenitor cells of the external granular layer, in internal granular layer cells at least as early as PND 7, and in Purkinje cells beginning at PND 14. Double-labeling immunofluorescence of brain sections with anti-BSC2 antibody and cell type-specific antibodies confirmed expression of the cotransporter gene product in neurons and oligodendrocytes in the white matter in a pattern similar to that determined by in situ hybridization. The temporal pattern of expression of the Na-K-2Cl cotransporter in the postnatal rat brain supports the hypothesis that the cotransporter is the mechanism of intracellular Cl− accumulation in immature neurons and oligodendrocytes. © 1997 John Wiley & Sons, Inc. J Neurobiol 33: 781–795, 1997 相似文献
7.
Interaction of neuron-specific K+-Cl- cotransporter, KCC2, with brain-type creatine kinase 总被引:2,自引:0,他引:2
gamma-Aminobutyric acid, a major inhibitory neurotransmitter within the adult central nervous system, is also known to be excitatory at early developmental stages due to the elevated intracellular Cl(-) concentration. This functional change is primarily attributable to a K(+)-Cl(-) cotransporter, KCC2, the expression of which is developmentally regulated in neurons. However, little detail information is available concerning the intracellular regulation of KCC2 function. Here, we identify an interaction between KCC2 and brain-type creatine kinase by means of yeast two-hybrid screening. This interaction, which was also detected in cultured cells and brain extracts, might contribute to KCC2-mediated modulation of Cl(-) homeostasis. 相似文献
8.
Casula S Shmukler BE Wilhelm S Stuart-Tilley AK Su W Chernova MN Brugnara C Alper SL 《The Journal of biological chemistry》2001,276(45):41870-41878
K-Cl cotransport regulates cell volume and chloride equilibrium potential. Inhibition of erythroid K-Cl cotransport has emerged as an important adjunct strategy for the treatment of sickle cell anemia. However, structure-function relationships among the polypeptide products of the four K-Cl cotransporter (KCC) genes are little understood. We have investigated the importance of the N- and C-terminal cytoplasmic domains of mouse KCC1 to its K-Cl cotransport function expressed in Xenopus oocytes. Truncation of as few as eight C-terminal amino acids (aa) abolished function despite continued polypeptide accumulation and surface expression. These C-terminal loss-of-function mutants lacked a dominant negative phenotype. Truncation of the N-terminal 46 aa diminished function. Removal of 89 or 117 aa (Delta(N)117) abolished function despite continued polypeptide accumulation and surface expression and exhibited dominant negative phenotypes that required the presence of the C-terminal cytoplasmic domain. The dominant negative loss-of-function mutant Delta(N)117 was co-immunoprecipitated with wild type KCC1 polypeptide, and its co-expression did not reduce wild type KCC1 at the oocyte surface. Delta(N)117 also exhibited dominant negative inhibition of human KCC1 and KCC3 and, with lower potency, mouse KCC4 and rat KCC2. 相似文献
9.
Functional interaction of the K-Cl cotransporter (KCC1) with the Na-K-Cl cotransporter in HEK-293 cells 总被引:2,自引:0,他引:2
Gillen Christopher M.; Forbush Bliss III 《American journal of physiology. Cell physiology》1999,276(2):C328
We have studiedthe regulation of the K-Cl cotransporter KCC1 and its functionalinteraction with the Na-K-Cl cotransporter. K-Cl cotransporter activitywas substantially activated in HEK-293 cells overexpressing KCC1(KCC1-HEK) by hypotonic cell swelling, 50 mM external K, andpretreatment with N-ethylmaleimide(NEM). Bumetanide inhibited 86Rbefflux in KCC1-HEK cells after cell swelling [inhibition constant (Ki) ~190µM] and pretreatment with NEM(Ki ~60 µM).Thus regulation of KCC1 is consistent with properties of the red cellK-Cl cotransporter. To investigate functional interactions between K-Cland Na-K-Cl cotransporters, we studied the relationship between Na-K-Clcotransporter activation and intracellular Cl concentration([Cl]i). Without stimulation, KCC1-HEK cells had greater Na-K-Cl cotransporter activitythan controls. Endogenous Na-K-Cl cotransporter of KCC1-HEK cells wasactivated <2-fold by low-Cl hypotonic prestimulation, compared with10-fold activation in HEK-293 cells and >20-fold activation in cellsoverexpressing the Na-K-Cl cotransporter (NKCC1-HEK). KCC1-HEK cellshad lower resting[Cl]i than HEK-293cells; cell volume was not different among cell lines. We found a steeprelationship between[Cl]i and Na-K-Clcotransport activity within the physiological range, supporting aprimary role for [Cl]iin activation of Na-K-Cl cotransport and in apical-basolateral crosstalk in ion-transporting epithelia. 相似文献
10.
K-Cl cotransporter expression in the human kidney 总被引:2,自引:0,他引:2
Liapis Helen; Nag M.; Kaji Deepak M. 《American journal of physiology. Cell physiology》1998,275(6):C1432
The K-Cl cotransporter protein KCC1 is a membrane transportprotein that mediates the coupled, electroneutral transport of K and Clacross plasma membranes. The precise cell type(s) in the kidney thatexpress the K-Cl cotransporter have remained unknown. The aim of thepresent investigation was to define the distribution of KCC1 mRNA inthe human kidney. We used in situ hybridization with a nonradioactivedigoxigenin-labeled riboprobe. We identified abundant KCC1 mRNAexpression in the epithelial cells throughout the distal and proximalrenal tubular epithelium. The transporter was also expressed inglomerular mesangial cells and endothelial cells of the renal vessels.These findings suggest that the K-Cl cotransporter may have animportant role in transepithelial K and Cl reabsorption. 相似文献
11.
BDNF-induced TrkB activation down-regulates the K+-Cl- cotransporter KCC2 and impairs neuronal Cl- extrusion 总被引:7,自引:0,他引:7
Rivera C Li H Thomas-Crusells J Lahtinen H Viitanen T Nanobashvili A Kokaia Z Airaksinen MS Voipio J Kaila K Saarma M 《The Journal of cell biology》2002,159(5):747-752
Pathophysiological activity and various kinds of traumatic insults are known to have deleterious long-term effects on neuronal Cl- regulation, which can lead to a suppression of fast postsynaptic GABAergic responses. Brain-derived neurotrophic factor (BDNF) increases neuronal excitability through a conjunction of mechanisms that include regulation of the efficacy of GABAergic transmission. Here, we show that exposure of rat hippocampal slice cultures and acute slices to exogenous BDNF or neurotrophin-4 produces a TrkB-mediated fall in the neuron-specific K+-Cl- cotransporter KCC2 mRNA and protein, as well as a consequent impairment in neuronal Cl- extrusion capacity. After kindling-induced seizures in vivo, the expression of KCC2 is down-regulated in the mouse hippocampus with a spatiotemporal profile complementary to the up-regulation of TrkB and BDNF. The present data demonstrate a novel mechanism whereby BDNF/TrkB signaling suppresses chloride-dependent fast GABAergic inhibition, which most likely contributes to the well-known role of TrkB-activated signaling cascades in the induction and establishment of epileptic activity. 相似文献
12.
Li H Khirug S Cai C Ludwig A Blaesse P Kolikova J Afzalov R Coleman SK Lauri S Airaksinen MS Keinänen K Khiroug L Saarma M Kaila K Rivera C 《Neuron》2007,56(6):1019-1033
The neuron-specific K-Cl cotransporter, KCC2, induces a developmental shift to render GABAergic transmission from depolarizing to hyperpolarizing. Now we demonstrate that KCC2, independently of its Cl(-) transport function, is a key factor in the maturation of dendritic spines. This morphogenic role of KCC2 in the development of excitatory synapses is mediated by structural interactions between KCC2 and the spine cytoskeleton. Here, the binding of KCC2 C-terminal domain to the cytoskeleton-associated protein 4.1N may play an important role. A more general conclusion based on our data is that KCC2 acts as a synchronizing factor in the functional development of glutamatergic and GABAergic synapses in cortical neurons and networks. 相似文献
13.
Race Joanne E.; Makhlouf Fadi N.; Logue Paul J.; Wilson Frederick H.; Dunham Philip B.; Holtzman Eli J. 《American journal of physiology. Cell physiology》1999,277(6):C1210
We isolated and characterized a novelK-Cl cotransporter, KCC3, from human placenta. The deduced proteincontains 1,150 amino acids. KCC3 shares 75-76% identity at theamino acid level with human, pig, rat, and rabbit KCC1 and 67%identity with rat KCC2. KCC3 is 40 and 33% identical to twoCaenorhabditis elegans K-Cl cotransporters and ~20%identical to other members of the cation-chloride cotransporter family(CCC), two Na-K-Cl cotransporters (NKCC1, NKCC2), and the Na-Clcotransporter (NCC). Hydropathy analysis indicates a typical KCCtopology with 12 transmembrane domains, a large extracellular loopbetween transmembrane domains 5 and 6 (unique to KCCs), and largeNH2 and COOH termini. KCC3 is predominantly expressed inkidney, heart, and brain, and is also expressed in skeletal muscle,placenta, lung, liver, and pancreas. KCC3 was localized to chromosome15. KCC3 transiently expressed in human embryonic kidney (HEK)-293cells fulfilled three criteria for increased expression of K-Clcotransport: stimulation of cotransport by swelling, treatment withN-ethylmaleimide, or treatment with staurosporine. 相似文献
14.
Several members of the cation-chloride cotransporter (solute carrier family 12, SLC12) gene family are expressed within the central nervous system, with one family member, the K+-Cl- cotransporter KCC2, exclusive to neurons. These transporters are best known for their roles in cell volume regulation and epithelial salt transport, but are increasingly receiving attention in neuroscience. In particular, intracellular chloride activity and hence the neuronal response to GABA and glycine appears to be determined by a balance between chloride efflux and influx through KCC2 and the Na+-K+-2Cl- cotransporter NKCC1, respectively. This relationship has important implications for neuronal development, sensory perception, neuronal excitability, and the response to neuronal injury. Finally, the association between loss of function in the K+-Cl- cotransporter KCC3, with a severe peripheral neuropathy associated with agenesis of the corpus callosum, has revealed an unexpected role for K+-Cl- cotransport in the development and/or maintenance of both the central and peripheral nervous systems. 相似文献
15.
16.
Inoue K Furukawa T Kumada T Yamada J Wang T Inoue R Fukuda A 《The Journal of biological chemistry》2012,287(25):20839-20850
GABA inhibits mature neurons and conversely excites immature neurons due to lower K(+)-Cl(-) cotransporter 2 (KCC2) expression. We observed that ectopically expressed KCC2 in embryonic cerebral cortices was not active; however, KCC2 functioned in newborns. In vitro studies revealed that taurine increased KCC2 inactivation in a phosphorylation-dependent manner. When Thr-906 and Thr-1007 residues in KCC2 were substituted with Ala (KCC2T906A/T1007A), KCC2 activity was facilitated, and the inhibitory effect of taurine was not observed. Exogenous taurine activated the with-no-lysine protein kinase 1 (WNK1) and downstream STE20/SPS1-related proline/alanine-rich kinase (SPAK)/oxidative stress response 1 (OSR1), and overexpression of active WNK1 resulted in KCC2 inhibition in the absence of taurine. Phosphorylation of SPAK was consistently higher in embryonic brains compared with that of neonatal brains and down-regulated by a taurine transporter inhibitor in vivo. Furthermore, cerebral radial migration was perturbed by a taurine-insensitive form of KCC2, KCC2T906A/T1007A, which may be regulated by WNK-SPAK/OSR1 signaling. Thus, taurine and WNK-SPAK/OSR1 signaling may contribute to embryonic neuronal Cl(-) homeostasis, which is required for normal brain development. 相似文献
17.
Sangan P Brill SR Sangan S Forbush B Binder HJ 《The Journal of biological chemistry》2000,275(40):30813-30816
Active potassium absorption in the rat distal colon is electroneutral, Na(+)-independent, partially chloride-dependent, and energized by an apical membrane H,K-ATPase. Both dietary sodium and dietary potassium depletion substantially increase active potassium absorption. We have recently reported that sodium depletion up-regulates H,K-ATPase alpha-subunit mRNA and protein expression, whereas potassium depletion up-regulates H,K-ATPase beta-subunit mRNA and protein expression. Because overall potassium absorption is non-conductive, K-Cl cotransport (KCC) at the basolateral membrane may also be involved in potassium absorption. Although KCC1 has not been cloned from the colon, we established, in Northern blot analysis with mRNA from the rat distal colon using rabbit kidney KCC1 cDNA as a probe, the presence of an expected size mRNA in the rat colon. This KCC1 mRNA is substantially increased by potassium depletion but only minimally by sodium depletion. KCC1-specific antibody identified a 155-kDa protein in rat colonic basolateral membrane. Potassium depletion but not sodium depletion resulted in an increase in KCC1 protein expression in basolateral membrane. The increase of colonic KCC1 mRNA abundance and KCC1 protein expression in potassium depletion of the rat colonic basolateral membrane suggests that K-Cl cotransporter: 1) is involved in transepithelial potassium absorption and 2) regulates the increase in potassium absorption induced by dietary potassium depletion. We conclude that active potassium absorption in the rat distal colon involves the coordinated regulation of both apical membrane H,K-ATPase and basolateral membrane KCC1 protein. 相似文献
18.
Pavel Uvarov Anastasia Ludwig Marika Markkanen Shetal Soni Christian A. H��bner Claudio Rivera Matti S. Airaksinen 《The Journal of biological chemistry》2009,284(20):13696-13704
The neuron-specific K-Cl cotransporter KCC2 maintains the low intracellular
chloride concentration required for the fast hyperpolarizing actions of
inhibitory neurotransmitters. The KCC2 gene codes for two isoforms, KCC2a and
KCC2b, which differ in their N termini. The relative expression and cellular
distribution of the two KCC2 protein isoforms are unknown. Here, we
characterize an antibody against the KCC2a isoform and show that a previously
described antibody against KCC2 is specific for the KCC2b isoform (Hubner, C.
A., Stein, V., Hermans-Borgmeyer, I., Meyer, T., Ballanyi, K., and Jentsch, T.
J. (2001) Neuron 30, 515–524). Immunostaining of dissociated
hippocampal cultures confirms that both KCC2 isoforms are neuron-specific.
Immunoblot analysis indicates that KCC2b is the major KCC2 isoform in the
adult brain, whereas in the neonatal mouse central nervous system, half of
total KCC2 protein is KCC2a. At this stage, the two KCC2 isoforms are largely
colocalized and show similar patterns of distribution in the brain. When
coexpressed in HEK293 cells, KCC2a and KCC2b proteins form heteromeric
complexes. Moreover, the two isoforms can be coimmunoprecipitated from the
neonatal brain, suggesting the presence of endogenous KCC2a-KCC2b heteromers.
Consistent with this, native gel analysis shows that a substantial part of
endogenous KCC2 isoforms in the neonatal brain constitute dimers.The neuron-specific K+-Cl- cotransporter KCC2
extrudes potassium and chloride ions from neurons, thus maintaining the low
intracellular chloride concentration [Cl]i necessary for
the fast hyperpolarizing actions of inhibitory neurotransmitters
γ-aminobutyric acid (GABA) and glycine
(1). KCC2 is an ∼140-kDa
plasma membrane protein that belongs to the cation chloride cotransporter
(CCC)4 family
(2,
3). CCCs, including KCC2, are
thought to exist in the plasma membrane as functional oligomers, although the
mechanisms whereby oligomerization affects their transport activity are
unclear
(4–8).We have recently shown that the KCC2 gene (alias Slc12a5)
generates two mRNAs, KCC2a and KCC2b, by an alternative promoter and first
exon usage (9). The difference
between the KCC2a and KCC2b proteins lies in the most N-terminal part; the 40
unique amino acids in KCC2a include a putative binding sequence for the
Ste20-related proline-alanine-rich kinase (SPAK). KCC2 null mutant mice
deficient for both KCC2 isoforms show a disrupted breathing rhythm and die
immediately after birth (10,
11), whereas selective KCC2b
isoform knock-out mice exhibit spontaneous seizures but can survive up to 3
weeks after birth (9,
12). Thus, KCC2a obviously
supports some vital functions of lower brain structures.In general, KCC2 expression in the CNS follows neuronal maturation; it is
first detected in the postmitotic neurons of the spinal cord and brainstem and
is then gradually increased in higher brain structures
(13,
14). Our previous work has
shown that during postnatal development, KCC2a mRNA expression remains
relatively constant, whereas KCC2b mRNA is strongly up-regulated in the cortex
during postnatal development
(9). This indicates that KCC2b
is responsible for the developmental shift from depolarizing to
hyperpolarizing GABAergic responses.Here, we study the relative expression and cellular distribution of KCC2a
and KCC2b proteins in the mouse brain. We characterize a new antibody specific
for the KCC2a isoform and demonstrate that a previously described antibody
against KCC2 (11) is specific
for the KCC2b isoform. The relative expression of the KCC2 protein isoforms
determined by immunoblot analysis correlates well with their mRNA levels
(9). KCC2a and KCC2b proteins
have a similar level and pattern of expression in the neonatal mouse brain and
are colocalized in most neurons in non-cortical lower brain areas.
Coimmunoprecipitation (coIP) experiments and coexpression followed by native
gel analysis indicate that KCC2a-KCC2b heteromers can form in vitro
and may exist in vivo. 相似文献
19.
Cloning, characterization, and chromosomal location of a novel human K+-Cl- cotransporter 总被引:4,自引:0,他引:4
Hiki K D'Andrea RJ Furze J Crawford J Woollatt E Sutherland GR Vadas MA Gamble JR 《The Journal of biological chemistry》1999,274(15):10661-10667
Differential display polymerase chain reaction has been used to isolate genes regulated in vascular endothelial cells by the angiogenic factor vascular endothelial cell growth factor (VEGF). Analysis of one of the bands consistently up-regulated by VEGF led us to the identification of a cDNA from a human umbilical vein endothelial cell library that is 77% identical to the human K+-Cl- cotransporter1 (KCC1). We have referred to the predicted protein as K+-Cl- cotransporter 3 (KCC3). Hydrophobicity analysis of the KCC3 amino acid sequence showed an almost identical pattern to KCC1, suggesting 12 membrane-spanning segments, a large extracellular loop with potential N-glycosylation sites, and cytoplasmic N- and C-terminal regions. The KCC3 mRNA was highly expressed in brain, heart, skeletal muscle, and kidney, showing a distinct pattern and size from KCC1 and KCC2. The KCC3 mRNA level in endothelial cells increased on treatment with VEGF and decreased with the proinflammatory cytokine tumor necrosis factor alpha, whereas KCC1 mRNA levels remained unchanged. Stable overexpression of KCC3 cDNA in HEK293 cells produced a glycoprotein of approximately 150 kDa, which was reduced to 120 kDa by glycosidase digestion. An increased initial uptake rate of 86Rb was seen in clones with high KCC3 expression, which was dependent on extracellular Cl- but not Na+ and was inhibitable by the loop diuretic agent furosemide. The KCC3 genomic localization was shown to be 15q13 by fluorescence in situ hybridization. Radiation hybrid analysis placed KCC3 within an area associated with juvenile myoclonic epilepsy. These results suggest KCC3 is a new member of the KCC family that is under distinct regulation from KCC1. 相似文献