稀土卟啉近红外发光配合物在生命科学领域中的应用研究进展

稀土近红外发光材料具有独特的光物理性质,如发光谱带窄、较大的Stock位移、荧光寿命长可达毫秒级等,在医学诊断和成像、免疫分析等热门领域具有重大的应用前景。但由于跃迁选择定则,稀土离子本身的吸收系数较小,需要用特定的生色团对其进行敏化,以增强其发光性能。在众多生色团中,卟啉化合物由于其激发态能级与近红外发光的稀土离子能级较为匹配,可以较好的敏化稀土离子,获得较高的近红外发光效率,因此,近年来受到了极大的关注。本文总结了近年来近红外发光卟啉稀土配合物在生命科学领域中的应用研究进展,并对其发展前景进行了展望。     

原卟啉Ⅸ发光特性与癌发光关系的研究

研究了人体内源性卟啉──原卟啉Ⅸ（ＰｒｏｔｏｐｏｒｐｈｙｒｉｎⅨ简称ＰＰ）的发光特性,结果表明,它与癌组织抽提物的发光及目前用于临床研究所选用的癌固有荧光特征峰其本相类同     

基于金属配合物的生物小分子发光探针研究进展

生物体内存在各种内源性活性物质,帮助生物进行信号传递与代谢调控。正常条件下,细胞环境不断变化,内源性小分子的时 空分布在生物体内保持动态平衡。但当它们的种类和浓度超过生理过程所需的限定范围时,就会影响细胞活性,进而导致疾病,甚至 是肿瘤和癌症的发生。因此,这些活性物质在体内活动的实时追踪及可视化对人们理解生命现象、研究疾病发生机制十分重要。与传 统有机染料相比,金属配合物发光(荧光/磷光)探针因光稳定性好、生理功能易调控等优势,已成为生物体系小分子活性物质示踪和 成像的研究热点。依照不同的作用靶点,对应用于生物体系的金属配合物探针的最新进展进行分类和总结,并展望金属配合物在生物 成像中的未来应用,以期可以为人们继续设计出新的具有良好示踪成像性能金属配合物探针提供参考,并从分子水平理解探针作用及 癌症治疗的机制。     

在光作用下卟啉和蛋白复合物的发光特性——癌固有荧光发光物质的探讨

本文研究了在光作用下,原卟啉和白蛋白复合物的发光特性,讨论了这些特性与临床诊断中所选择的癌固有荧光峰的关系。     

甾体-卟啉化学的研究——Ⅰ.雌甾-卟啉化合物的合成及炔雌醇-卟啉生理活性的研究

本文合成了8个新的雌甾-卟啉化合物。首次对炔雌醇-卟啉的生理活性进行了初步研究。     

萤火虫的发光行为及其功能起源

发光生物因其活体能自发荧光而倍受关注,萤火虫(鞘翅目:萤科)是被研究最多的发光生物,因为成虫发光在其性选择以及求偶交配中起着重要作用.由于萤火虫的发光是一个消耗能量(ATP),的化学反应过程,因而其发光在成虫之外的虫态也具有重要意义.本文就萤火虫成虫和幼虫的发光行为、功能意义进行了综述,并结合萤火虫的饲养观察对其卵和蛹的发光行为进行了描述,探讨了卵和蛹的形态阶段发光的生物学功能以及生物荧光的起源进化.这将有助于理解萤火虫及其它生物自发荧光的本质和起源进化过程.     

类卟啉稀土配合物对于小鼠腹水肝癌细胞光敏损伤的研究

研究了类卟啉稀土配合物（以下简称ＰＬＭ－Ｇｄ－Ａ）对小鼠腹水肝癌细胞（ＡＨ）的光敏作用及ＡＨ细胞对ＰＬＭ－Ｇｄ－Ａ的摄取表明：ＰＬＭ－Ｇｄ－Ａ被ＡＨ细胞摄取的速度快,用ＭＴＴ方法测定了细胞光敏存活曲线,杀伤细胞的能力与光照时间和光照强度以及ＰＬＭ－Ｇｄ的浓度密切相关,用ＦＡＤＵ方法和电镜观察结果证明：该光敏损伤细胞的靶主要是在细胞核;对于不同稀土离子配合物光敏能力比较表明：Ｇｄ＞Ｅｕ＞Ｓｍ;造成细     

类卟啉稀土配合物对于小鼠腹水肝癌细胞光敏损伤的研究

研究了类卟啉稀土配合物（以下简称ＰＬＭ—Ｇｄ—Ａ）对小鼠腹水肝癌细胞（ＡＨ）的光敏作用及ＡＨ细胞对ＰＬＭ一Ｇｄ—Ａ的摄取表明：ＰＬＭ－Ｇｄ－Ａ被ＡＨ细胞摄取的速度快（大约１０ｍｉｎ可达到平衡）,用ＭＴＴ方法测定了细胞光敏存活曲线,杀伤细胞的能力与光照时间和光照强度以及ＰＬＭ—Ｇｄ的浓度密切相关,用ＦＡＤＵ方法和电镜观察结果证明：该光敏损伤细胞的靶主要是在细胞核;对于不同稀土离子配合物光敏能力比较表明：Ｇｄ＞Ｅｕ＞Ｓｍ;造成细胞死亡的原因包括１Ｏ２、在内的活性氧。     

有机荧光探针辅助的近红外荧光成像技术在宫颈癌中的应用

荧光成像已被广泛应用于生物医学和临床诊断领域.近红外(Near-infrared,NIR,700-1700 nm)荧光成像在NIR波段对生物组织显影,与可见光波段(400-760 nm)的传统荧光成像相比,更有助于提高成像的信噪比和灵敏度.高质量的荧光成像需要借助良好的荧光探针,纳米技术的快速发展使具备良好荧光特性的有...     

Near Infrared,Highly Efficient Luminescent Solar Concentrators

The fabrication of a low reabsorption emission loss, high efficient luminescent solar concentrator (LSC) is demonstrated by embedding near infrared (NIR) core/shell quantum dots (QDs) in a polymer matrix. An engineered Stokes shift in NIR core/shell PbS/CdS QDs is achieved via a cation exchange approach by varying the core size and shell thickness through the refined reaction parameters such as reaction time, temperature, precursor molar ratio, etc. The as‐synthesized core/shell QDs with high quantum yield (QY) and excellent chemical/photostability exhibit a large Stokes shift with respect to the bare PbS QDs due to the strong core‐to‐shell electrons leakage. The large‐area planar LSC based on core/shell QDs exhibits the highest value (6.1% with a geometric factor of 10) for optical efficiency compared to the bare NIR QD‐based LSCs and other reported NIR QD‐based LSCs. The suppression of emission loss and the broad absorption of PbS/CdS QDs offer a promising pathway to integrate LSCs and photovoltaic devices with good spectral matching, indicating that the proposed core/shell QDs are strong candidates for fabricating high efficiency semi‐transparent large‐area LSCs.     

近红外光谱技术在稻米特性检测中的应用(综述)

近红外光谱技术是一种新型的检测分析技术,广泛应用于农业、林业、工业、医药以及食品等多个行业领域。文章综述近红外光谱技术在稻米特性检测中的应用概况,包括对大米淀粉、蛋白质和脂肪酸等营养物质的测定,大米糊化特性、粘稠度和食味特性的分析,水稻生长过程中氮、磷、钾和其他营养元素含量的分析,育种研究与品种鉴别,病害、重金属等有害物质以及其他方面。同时,指出该技术在当前检测应用中存在的一些问题,并针对目前发展趋势展望该技术的前景。     

近红外光谱技术（NIRS）在人体的应用与展望

近红外光谱技术(NIRS)在人体的应用与研究是近年来在国内外新兴的研究领域,因为其方便无创,成本低等优点,近20年来在不断发展和完善,引起大家的广泛关注。近红外光谱在700-900 mm范围内可以穿透一定深度的组织,组织内含氧血红蛋白、去氧血红蛋白对近红外光的吸收系数存在差异,经过传感器和计算机技术分析,得到组织的血氧参数。其测量参数为微动脉、微静脉和毛细血管中血液的血氧参数之加权平均,反应组织中的血氧参数,其中静脉血占主要地位,不同于普通脉搏式血氧监测仪的指端动脉血的血氧饱和度。基于近红外光谱技术的近红外组织血氧无创监测仪、功能性近红外光谱技术(f NIRS)、近红外光谱荧光技术等在临床医学,运动医学,神经生物学,认知科学,脑力疲劳,人机交互等新兴领域正发挥越来越重要作用。     

近红外光谱法无创测量人体血红蛋白浓度

在选定的波长处(760 nm,850 nm)无创测量人体手指的漫反射光谱,利用修正的Lambert-Beer定律,实现了HbO2、Hb的绝对量估算,并由此求得血红蛋白浓度的结果。对16例志愿者进行了测量,全部男性受试者的相对误差在7.39%以下,相关系数为0.954;全部女性受试者的相对误差在7.26%以下,相关系数为0.969。     

Spectral Analysis of Event-Related Hemodynamic Responses in Functional Near Infrared Spectroscopy

The goal of this paper is to design experiments that confirm the evidence of cognitive responses in functional near infrared spectroscopy and to establish relevant spectral subbands. Hemodynamic responses of brain during single-event trials in an odd-ball experiment are measured by functional near infrared spectroscopy method. The frequency axis is partitioned into subbands by clustering the time-frequency power spectrum profiles of the brain responses. The predominant subbands are observed to confine the 0–30 mHz, 30–60 mHz, and 60–330 mHz ranges. We identify the group of subbands that shows strong evidence of protocol-induced periodicity as well as the bands where good correlation with an assumed hemodynamic response models is found.     

Synthesis and Properties of a Fluorescent-Labeled Triglyceride Derivative of the Antitumor Drug Merphalan

A new fluorescent probe, a 3-perylenoyl derivative of the lipophilized antitumor drug merphalan (sarcolysine), was synthesized. The probe is suitable for studying intracellular traffic and metabolism of merphalan and its derivatives. The perylenoyl fluorescence is partially quenched by the merphalan chromophore, which broadens the probe potentialities.     

The structure of a far‐red fluorescent protein,AQ143, shows evidence in support of reported red‐shifting chromophore interactions

Engineering fluorescent proteins (FPs) to emit light at longer wavelengths is a significant focus in the development of the next generation of fluorescent biomarkers, as far‐red light penetrates tissue with minimal absorption, allowing better imaging inside of biological hosts. Structure‐guided design and directed evolution have led to the discovery of red FPs with significant bathochromic shifts to their emission. Here, we present the crystal structure of one of the most bathochromically shifted FPs reported to date, AQ143, a nine‐point mutant of aeCP597, a chromoprotein from Actinia equina. The 2.19 Å resolution structure reveals several important chromophore interactions that contribute to the protein's far‐red emission and shows dual occupancy of the green and red chromophores.     

Purification and Properties of Deoxyribonuclease B From Bacillus subtilis

An exonuclease, DNase B, was isolated from Bacillus subtilis Marburg strain. Molecular weight of DNase B was estimated to be 200,000 by glycerol gradient centrifugation, however, 56,000 by SDS-polyacrylamide gel electrophoresis. This result would indicate a possibility that the enzyme consisted of an identical subunit. The enzyme was specific for single-stranded DNA, required Mg²⁺ or Mn²⁺ (5 mm) for the maximal activity, but 40% of the activity was retained in the absence of divalent cations. The enzyme was active even in the presence of EDTA. The enzyme degraded single-stranded DNA exonucleolytically, producing oligonucleotides in the direction from the 5′-end to the 3′-end.     

Synthesis,Photophysical Properties,and Cytotoxicity of Rhodamine Based Fluorescent Probes

Russian Journal of Bioorganic Chemistry - Rhodamine derivatives are heterocyclic compound, related to the fluorescent probe having a profound application in the field of biotechnology. Herein, we...