共查询到20条相似文献,搜索用时 15 毫秒
1.
Satellite RNA-mediated reduction of cucumber mosaic virus genomic RNAs accumulation in Nicotiana tabacum 总被引:1,自引:0,他引:1
Satellite RNAs(satRNAs)are molecular parasites that interfere with the pathogenesis of thehelper viruses.In this study,the relative accumulation of cucumber mosaic virus(CMV)-Fny genomicRNAs with or without satRNAs were quantitatively analyzed by real-time RT-PCR.The results showed thatsatRs apparently attenuated the symptoms of CMV-Fny on Nicotiana tabacum by depressing the accumu-lation of CMV-Fny genomic RNAs,tested as open reading frames.The accumulation of CMV-Fny 1a,2a,2b,3a,and CP genes was much higher than that of CMV-Fny with satRs added(CMV-Fsat),at differentinoculation times.CMV-FnyΔ2b,in which the complete 2b gene and 41 amino acids at the C-terminal of the2a gene were deleted,caused only a slight mosaic effect on N.tabacum seedlings,similar to that of CMV-Fsat,but the addition of satRs to CMV-FnyΔ2b showed further decrease in the accumulation of CMV-FnyΔ2b genomic RNAs.Our results indicated that the attenuation of CMV,by adding satRs or deleting the2b gene,was due to the low accumulation of CMV genomic RNAs,and that satRNA-mediated reduction ofCMV genomic RNAs accumulation in N.tabacum was possibly related to the 2b gene. 相似文献
2.
The amino acid sequences of the non-structural protein (molecular weight 35,000; 3a protein) from three plant viruses — cucumber
mosaic, brome mosaic and alfalfa mosaic have been systematically compared using the partial genomic sequences for these three
viruses already available. The 3a protein of cucumber mosaic virus has an amino acid sequence homology of 33.7% with the corresponding
protein of brome mosaic virus. A similar protein from alfalfa mosaic virus has a homology of 18.2% and 14.2% with the protein
from brome mosaic virus and cucumber mosaic virus, respectively. These results suggest that the three plant viruses are evolutionarily
related, although, the evolutionary distance between alfalfa mosaic virus and cucumber mosaic virus or brome mosaic virus
is much larger than the corresponding distance between the latter two viruses. 相似文献
3.
Characterization of Synergy between Cucumber mosaic virus and Tobacco necrosis virus in Nicotiana benthamiana 总被引:1,自引:0,他引:1
D. Xi H. Feng L. Lan J. Du J. Wang Z. Zhang L. Xue W. Xu H. Lin 《Journal of Phytopathology》2007,155(9):570-573
Mixed infections of Nicotiana benthamiana plants by Cucumber mosaic virus (CMV) and Tobacco necrosis virus (TNV) exhibit a synergistic interaction and result in symptom enhancement. Accumulation of CMV(+) RNA as well as capsid protein (CP) in mixed infection was considerably higher than that of singly‐infected plants. There was also a slight increase in TNV(+) RNA and CP levels in doubly infected plants. Synergistic infection by CMV‐ and TNV‐induced higher increase in the levels of malonyldialdehyde, hydrogen peroxide (H2O2) and more decline in the activities of catalase than singly infected ones. Both peroxidase and superoxide dismutase activities increased rapidly for the first 10 days post inoculation (dpi) in doubly‐infected plants and then declined, whereas the enzyme activities continued to increase after 10 dpi in singly infected plants and had higher enzyme activities in the late stages than that of co‐infected plants. These results suggest that synergistic infection by CMV and TNV produced severes oxidative stress in N. benthamiana plants and the synergy between the two viruses was mutual. 相似文献
4.
Exogenously applied dsRNA molecules deriving from the Zucchini yellow mosaic virus (ZYMV) genome move systemically and protect cucurbits against ZYMV
下载免费PDF全文
![点击此处可从《Molecular Plant Pathology》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Athanasios Kaldis Margarita Berbati Ourania Melita Chrysavgi Reppa Maria Holeva Patricia Otten Andreas Voloudakis 《Molecular Plant Pathology》2018,19(4):883-895
5.
Extreme resistance to cucumber mosaic virus (CMV) in transgenic tomato expressing one or two viral coat proteins 总被引:2,自引:0,他引:2
Kaniewski Wojciech Ilardi Vincenza Tomassoli Laura Mitsky T. Layton J. Barba Marina 《Molecular breeding : new strategies in plant improvement》1999,5(2):111-119
For the production of broad commercial resistance to cucumber mosaic virus (CMV) infection, tomato plants were transformed
with a combination of two coat protein (CP) genes, representing both subgroups of CMV. The CP genes were cloned from the CMV-D
strain and Italian CMV isolates (CMV-22 of subgroup I and CMV-PG of subgroup II) which have been shown to produce severe disease
symptoms. Four plant transformation vectors were constructed: pMON18774 and pMON18775 (CMV-D CP), pMON18831 (CMV-PG CP) and
pMON18833 (CMV-22 CP and CMV-PG CP). Transformed R0 plants were produced and lines were selected based on the combination
of three traits: CMV CP expression at the R0 stage, resistance to CMV (subgroup I and/or II) infection in growth chamber tests
in R1 expressing plants, and single transgene copy, based on R1 segregation. The results indicate that all four vector constructs
generated plants with extremely high resistant to CMV infection. The single and double gene vector construct produced plants
with broad resistance against strains of CMV from both subgroups I and II at high frequency. The engineered resistance is
of practical value and will be applied for major Italian tomato varieties.
This revised version was published online in June 2006 with corrections to the Cover Date. 相似文献
6.
7.
The complete nucleotide (nt) sequence (3369 nt) of RNA 1 of cucumber mosaic virus strain O (CMV-O) was determined. One open reading frame (ORF; 993 aa) could be deduced from the nt sequence. The homologies of the ORF between CMV-O and CMV-Q or CMV-Fny were calculated to be 85% or 97%, respectively. For CMV-O and CMV-Q, the first one-third of the ORF showed a higher degree of homology (89%), as compared with the other portions (82-85%); the first 224 aa showed more than 93% homology. A comparative study of the three viruses revealed that CMV-O is more homologous to CMV-Fny (subgroup I) [corrected]) than to CMV-Q (subgroup II) [corrected]. 相似文献
8.
Fuchs Marc Klas Ferdinand E. McFerson James R. Gonsalves Dennis 《Transgenic research》1998,7(6):449-462
Transgenic melon and squash containing the coat protein (CP) gene of the aphid transmissible strain WL of cucumber mosaic cucumovirus (CMV) were grown under field conditions to determine if they would assist the spread of the aphid non-transmissible strain C of CMV, possibly through heterologous encapsidation and recombination. Transgenic melon were susceptible to CMV strain C whereas transgenic squash were resistant although the latter occasionally developed chlorotic blotches on lower leaves. Transgenic squash line ZW-20, one of the parents of commercialized cultivar Freedom II, which expresses the CP genes of the aphid transmissible strains FL of zucchini yellow mosaic (ZYMV) and watermelon mosaic virus 2 (WMV 2) potyviruses was also tested. Line ZW-20 is resistant to ZYMV and WMV 2 but is susceptible to CMV. Field experiments conducted over two consecutive years showed that aphid-vectored spread of CMV strain C did not occur from any of the CMV strain C-challenge inoculated transgenic plants to any of the uninoculated CMV-susceptible non- transgenic plants. Although CMV was detected in 3% (22/764) of the uninoculated plants, several assays including ELISA, RT- PCR-RFLP, identification of CP amino acid at position 168, and aphid transmission tests demonstrated that these CMV isolates were distinct from strain C. Instead, they were non-targeted CMV isolates that came from outside the field plots. This is the first report on field experiments designed to determine the potential of transgenic plants expressing CP genes for triggering changes in virus-vector specificity. Our results indicate that transgenic plants expressing CP genes of aphid transmissible strains of CMV, ZYMV, and WMV 2 are unlikely to mediate the spread of aphid non-transmissible strains of CMV. This finding is of practical relevance because transgenic crops expressing the three CP genes are targeted for commercial release, and because CMV is economically important, has a wide host range, and is widespread worldwide. 相似文献
9.
Cymbidium mosaic virus (CyMV) is the most prevalent virus infecting orchids. Here, we report the isolation of partial cDNA clones encoding the genomic RNA of CyMV. Like most of the polyadenylated monopartite positive-strand RNA viruses, the open reading frame (ORF) coding for the viral coat protein (CP) is located at the 3 end. The ORF predicts a polypeptide chain of 220 amino acids with a molecular weight of 23 600. Sequence comparison of this ORF to the CP sequences of potato virus X(PVX) and white clover mosaic virus (WCIMV) revealed a strong amino acid homology in the mid-portion of the CP, but the overall homology was low. The CyMV CP gene was placed downstream of a cauliflower mosaic virus 35S promoter and the chimaeric gene was transferred into Nicotiana benthamiana. Transgenic plants expressing the CyMV CP were protected against CyMV infection. 相似文献
10.
Multiple alleles for zucchini yellow mosaic virus resistance at the zym locus in cucumber 总被引:6,自引:0,他引:6
E. Kabelka Z. Ullah R. Grumet 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1997,95(5-6):997-1004
Sources of resistance to several potyviruses have been identified and characterized within the cucumber (Cucumis sativus L.) germplasm. Resistance to zucchini yellow mosaic virus (ZYMV) is present in inbred lines derived from the Dutch hybrid
Dina (Dina-1) and from the Chinese cultivar ‘Taichung Mou Gua’ (TMG-1). Tests of allelism indicated that the genes for resistance
to ZYMV in TMG-1 and Dina-1 are at the same locus; however, the two genotypes exhibited different phenotypes in response to
cotyledon inoculation with ZYMV. Dina-1 exhibited a distinct veinal chlorosis and accumulation of virus limited to the first
and/or second true leaves, while TMG-1 remained symptom-free and did not accumulate virus. The distinct veinal chlorosis phenotype
in Dina-1 was dominant to the symptom-free phenotype in TMG-1 and was shown not to be due to a separate gene. These results
indicate that a series of alleles differing in effectiveness and dominance relationships occurs at the zym locus such that Zym>zym
Dina>zym
TMG-1. In addition to ZYMV resistance, TMG-1 is also resistant to watermelon mosaic virus (WMV), the watermelon strain of papaya
ringspot virus (PRSV-W) and the Moroccan watermelon mosaic virus (MWMV); the WMV and MWMV resistances are at the same locus,
or tightly linked to the zym locus. Dina-1 also was found to be resistant to PRSV-W and MWMV. The gene for MWMV resistance in Dina-1 appeared to be at
the same locus or tightly linked (<1% recombination) to the gene for ZYMV resistance. In contrast to the response to ZYMV
inoculation, Dina-1 does not exhibit distinct veinal chlorosis when inoculated with PRSV-W or MWMV. Collectively, these observations
suggest that the gene(s) conferring resistance to ZYMV, WMV, and MWMV may be part of a gene cluster for potyvirus resistance
in cucumber.
Received: 12 November 1996 / Accepted: 25 April 1997 相似文献
11.
《Seminars in Virology》1993,4(6):357-361
Transgenic tobacco plants (CP +) that express the coat protein gene of cucumber mosaic virus (CMV)-Y strain were highly protected from infection with either CMV virions or CMV RNA, while transgenic protoplasts were also protected from infection with CMV virions but not with CMV RNA. CP + plants showed greater susceptibility to infection with satellite RNA-free CMV-Y than CMV-Y containing satellite RNA. At temperatures above 30°C, CP + plants did not or poorly resist infection with CMV. Elevated temperature affected the accumulation of CP rather than its mRNA, suggesting that CP molecules are mainly involved in virus resistance in CP + plants. 相似文献
12.
13.
Cucumber mosaic virus genome is encapsidated in alfalfa mosaic virus coat protein expressed in transgenic tobacco plants 总被引:1,自引:0,他引:1
The expression of viral coat protein (CP) in transgenic plants has been shown to be very effective in virus plant protection. However, the introduction of CP genes into plants presents the potential risk of the encapsidation of a superinfecting viral genome in the transgenic protein, an event which could change the epidemiology of the disease. To detect the potential heterologous encapsidation of the cucumber mosaic virus (CMV) genome by alfalfa mosaic virus (AIMV) CP expressed in transgenic tobacco plants, a system of immunocapture (IC) and amplification by polymerase chain reaction (PCR) was optimized. This provided high sensitivity and reliable selection of the heterologously encapsidated CMV genome in the presence of natural CMV particles. As little as 2 pg of virus could be detected by immunocapture/polymerase chain reaction (IC/PCR) technique. Evidence for heterologous encapsidation of the CMV genome was found in 11 of the 33 transgenic plants tested two weeks after CMV inoculation. This demonstrates a significant rate of heterologous encapsidation events between two unrelated viruses in transgenic plants. Since CP is involved in the interactions of the virus particle with its vector, the release in the field of such transgenic plants could alter the transmission properties of some important viruses. 相似文献
14.
Thehypersensitiveresponse(HR)isoneofmostextensivelystudiedresistantreactionsdur-ingtheincompatibleinteractionbetweenplantandpathogen.Inthisprocess,plantinitiatesdiversedefensesystemssuchasdepositionofligninandcalloseinthecellwall,productionofphytoalex-ins,expressionofpathogenesis-relatedproteins(PRprotein)andactivationofprogrammedcelldeath(PCD),whichresultinlimitationofthepathogenwithintheinitialinfectionsites[1,2].TheHRinducedbybacteria,fungiandvirusesusuallyactivatesasystemicacquiredresis… 相似文献
15.
Ryu CM Murphy JF Mysore KS Kloepper JW 《The Plant journal : for cell and molecular biology》2004,39(3):381-392
Arabidopsis thaliana ecotype Columbia plants (Col-0) treated with plant growth-promoting rhizobacteria (PGPR) Serattia marcescens strain 90-166 and Bacillus pumilus strain SE34 had significantly reduced symptom severity by Cucumber mosaic virus (CMV). In some cases, CMV accumulation was also significantly reduced in systemically infected leaves. The signal transduction pathway(s) associated with induced resistance against CMV by strain 90-166 was determined using mutant strains and transgenic and mutant Arabidopsis lines. NahG plants treated with strains 90-166 and SE34 had reduced symptom severity indicating that the resistance did not require salicylic acid (SA). Strain 90-166 naturally produces SA under iron-limited conditions. Col-0 and NahG plants treated with the SA-deficient mutant, 90-166-1441, had significantly reduced CMV symptom severity with reduced virus accumulation in Col-0 plants. Another PGPR mutant, 90-166-2882, caused reduced disease severity in Col-0 and NahG plants. In a time course study, strain 90-166 reduced virus accumulation at 7 but not at 14 and 21 days post-inoculation (dpi) on the non-inoculated leaves of Col-0 plants. NahG and npr1-1 plants treated with strain 90-166 had reduced amounts of virus at 7 and 14 dpi but not at 21 dpi. In contrast, no decrease in CMV accumulation occurred in strain 90-166-treated fad3-2 fad7-2 fad8 plants. These data indicate that the protection of Arabidopsis against CMV by strain 90-166 follows a signaling pathway for virus protection that is independent of SA and NPR1, but dependent on jasmonic acid. 相似文献
16.
Fuchs Marc McFerson James R. Tricoli David M. McMaster J. Russell Deng Rosaline Z. Boeshore Maury L. Reynolds John F. Russell Paul F. Quemada Hector D. Gonsalves Dennis 《Molecular breeding : new strategies in plant improvement》1997,3(4):279-290
Cantaloupe line CZW-30 containing coat protein gene constructs of cucumber mosaic cucumovirus (CMV), zucchini yellow mosaic potyvirus (ZYMV), and watermelon mosaic virus 2 potyvirus (WMV-2) was investigated in the field over two consecutive years for resistance to infections by CMV, ZYMV, and/or WMV-2. Resistance was evaluated under high disease pressure achieved by mechanical inoculations and/or natural challenge inoculations by indigenous aphid vectors. Across five different trials, homozygous plants were highly resistant in that they never developed systemic symptoms as did the nontransformed plants but showed few symptomatic leaves confined close to the vine tips. Hemizygous plants exhibited a significant delay (2–3 weeks) in the onset of disease compared to control plants but had systemic symptoms 9–10 weeks after transplanting to the field. Importantly, ELISA data revealed that transgenic plants reduced the incidence of mixed infections. Only 8% of the homozygous and 33% of the hemizygous plants were infected by two or three viruses while 99% of the nontransformed plants were mixed infected. This performance is of epidemiological significance. In addition, control plants were severely stunted (44% reduction in shoot length) and had poor fruit yield (62% loss) compared to transgenic plants, and most of their fruits (60%) were unmarketable. Remarkably, hemizygous plants yielded 7.4 times more marketable fruits than control plants, thus suggesting a potential commercial performance. This is the first report on extensive field trials designed to assess the resistance to mixed infection by CMV, ZYMV, and WMV-2, and to evaluate the yield of commercial quality cantaloupes that are genetically engineered. 相似文献
17.
Three synthetic genes for the production of anti-sense RNA to different regions of the cucumber mosaic virus (CMV) genome were constructed using virus-derived double-stranded cDNA coupled to a promoter sequence from cauliflower mosaic virus. The genes were used to transform tobacco plants by a Ti plasmid vector. Transgenic plants obtained with the three constructs produced anti-sense RNA at different levels. Plants expressing each of the three anti-sense RNAs were inoculated with CMV and their sensitivity to the virus infection was compared with the non-transformed plants. Only one plant line which expressed relatively low levels of one of the anti-sense RNAs showed resistance to CMV but other plants expressing the same or the other two antisense RNAs had similar sensitivity to CMV infection as the non-transformed plants. 相似文献
18.
Mascia T Cillo F Fanelli V Finetti-Sialer MM De Stradis A Palukaitis P Gallitelli D 《Molecular plant-microbe interactions : MPMI》2010,23(11):1514-1524
Mixed infection with the SON41 strain of Potato virus Y (PVY-SON41) in tomato increased accumulation of RNAs of strains Fny and LS of Cucumber mosaic virus (CMV-Fny and CMV-LS, respectively) and enhanced disease symptoms. By contrast, replication of PVY-SON41 was downregulated by CMV-Fny and this was due to the CMV-Fny 2b protein. The CMV-FnyΔ2b mutant was unable to systemically invade the tomato plant because its movement was blocked at the bundle sheath of the phloem. The function needed for invading the phloem was complemented by PVY-SON41 in plants grown at 22°C whereas this complementation was not necessary in plants grown at 15°C. Mutations in the 2b protein coding sequence of CMV-Fny as well as inhibition of translation of the 2a/2b overlapping region of the 2a protein lessened both the accumulation of viral RNAs and the severity of symptoms. Both of these functions were complemented by PVY-SON41. Infection of CMV-Fny supporting replication of the Tfn-satellite RNA reduced the accumulation of CMV RNA and suppressed symptom expression also in plants mixed-infected with PVY-SON41. The interaction between CMV and PVY-SON41 in tomato exhibited different features from that documented in other hosts. The results of this work are relevant from an ecological and epidemiological perspective due to the frequency of natural mixed infection of CMV and PVY in tomato. 相似文献
19.
Viral infections generally cause disease symptoms by interfering with the microRNA (miRNA)-mediated regulation of gene expression
of host plants. In tomato leaves, the accumulation levels of eleven miRNAs and ten target mRNAs were enhanced by different
degrees upon Cucumber mosaic virus (CMV)-Fny and Tomato aspermy virus (TAV)-Bj infections. The ability of CMV-Fny to interfere with miRNA pathway was dramatically suppressed in the addition of
the benign satellite (sat) RNA variant (satYn12), but was slightly affected when CMV-Fny was co-inoculated with the aggressive satRNA variant (satT1). In plants harboring the infection of CMV-FnyΔ2b (a CMV-Fny 2b-deletion mutant), the unaltered miRNAs and target mRNAs
levels compared with mock inoculated plants indicated that 2b ORF was essential for perturbation of miRNA metabolism. When
the amounts of viral open reading frames (ORFs) in these infections were quantified, we found satYn12 caused a higher reduction of CMV-Fny accumulation levels than satT1. These results indicate the complex mechanism by which satRNAs participate in CMV-tomato interaction, and suggest that the severity of disease symptoms positively correlates to some
extent with the perturbation of miRNA pathway in tomato. 相似文献
20.
Tomassoli Laura Ilardi Vincenza Barba Marina Kaniewski Wojciech 《Molecular breeding : new strategies in plant improvement》1999,5(2):121-130
Since the summer of 1993, transgenic tomato plants expressing the coat protein (CP) genes of cucumber mosaic cucumovirus have
been tested under field conditions to assess the level of resistance and agronomic performance. Trials were performed in different
areas in Italy and the target virus in the majority of tests was spread naturally by the indigenous aphid populations. Twenty-three
homozygous lines of variety UC82B, transformed to contain four different CP genes of CMV, were evaluated. The lines were preselected
for CP expression, single gene copy, and virus resistance in growth chamber experiments. In general, CMV resistance was confirmed
under field conditions though resistance in the field was less effective than what was observed in growth chamber experiments.
The resistance observed in multi-year and multi-location experiments is of commercial value for several of the most resistant
lines. Engineered resistance upon transfer to Italian varieties by breeding or direct transformation will be used in tomato
production in Italy or elsewhere.
This revised version was published online in June 2006 with corrections to the Cover Date. 相似文献