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RNA synthesis: strategies for the use of bacteriophage RNA polymerases 总被引:14,自引:0,他引:14
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RNA polymerases A and B from Physarum were more active on denatured homologous, calf thymus, or phage DNA than on the corresponding native templates. We obtained distinct patterns of template activities for various single- and double-stranded synthetic homopolymers and alternating copolymers. Some templates were copied asymmetrically. All dC-rich structures were highly active templates. Poly(dA) was efficiently transcribed only in combination with oligo(dT), not with poly(dT). Differential activities of enzymes A and B on several synthetic templates and phage DNA suggest different requirements for the RNA synthesis by the two RNA polymerases from Physarum. 相似文献
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Reverse transcription of phage RNA and its fragment directed by synthetic heteropolymeric primers 总被引:1,自引:1,他引:0
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L. Yu. Frolova V. G. Metelyev K. I. Ratmanova V. D. Smirnov Z. A. Shabarova M. A. Prokofyev V. M. Berzin I. V. Jansone E. J. Gren L. L. Kisselev 《Nucleic acids research》1977,4(7):2145-2159
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Transcription of chromatin by human RNA polymerase 总被引:3,自引:0,他引:3
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