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1.
The taste organ in insects is a hair-shaped taste sensory unit having four functionally differentiated contact chemoreceptor cells. In the blowfly, Phormia regina, cGMP has been suggested to be a second messenger for the sugar receptor cell. Generally, cGMP is produced by membranous or soluble guanylyl cyclase (sGC), which can be activated by nitric oxide (NO). In the present paper, we electrophysiologically showed that an NO scavenger, 2-phenyl-4,4,5,5-tetramethylimidazoline-3-oxide-1-oxyl (PTIO), an NO donor, 1-hydroxy-2-oxo-3-(N-methyl-3-aminopropyl)-3-methyl-1-triazene (NOC 7) or an NO synthase (NOS) inhibitor, NG-nitro-L-arginine methyl ester (L-NAME) specifically affected the response in the sugar receptor cell, but not in other receptor cells. PTIO, when introduced into the receptor cells in a sensillum aided by sodium deoxycholate (DOC, pH 7.2), depressed the response of sugar receptor cells to sucrose but did not affect those of the salt or water receptor cells. NOC 7, given extracellularly, latently induced the response of sugar receptor cells; and L-NAME, when introduced into the receptor cells, depressed the response of sugar receptor cells. The results clearly suggest that NO, which may be produced by intrinsic NOS in sugar receptor cells, participates in the transduction cascade of these cells in blowfly. 相似文献
2.
Ahamed A Tsurumi S Ozaki M Amakawa T 《Comparative biochemistry and physiology. Part A, Molecular & integrative physiology》2000,125(3):343-349
Chromosaponin I (CSI), a gamma-pyronyl-triterpenoid saponin isolated from pea and other leguminous plants, stimulates the growth of roots in a variety of plants. In the present work, we introduce CSI as a sugar taste substance for the blowfly, Phormia regina. The blowfly has taste chemosensilla on the labellum. The sensory receptor cells in the chemosensillum are highly specialized for the tastes of sugar, salt and water, respectively. Application of CSI induced the feeding response of blowflies including full proboscis extension. CSI also induced impulses of the sugar taste receptor cell in the LL-type sensillum. The optimum concentration of CSI in these responses was 0.1 mM which is much lower than that of sucrose. Based on the comparison of dose-response relationships, CSI is 100 times more effective than sucrose in stimulating the sugar taste receptor cells. CSI-induced impulses appeared after a significant latency compared with sucrose. As far as we know, this is the first report describing that a natural saponin induces sugar responses in insects. CSI is a unique saponin because of its bifunctional property in plants and insects. 相似文献
3.
We studied the birth times of neurons of labellar taste sensilla in blowflies using incorporation of the thymidine analogue 5-bromodeoxyuridine (BrdU) as an indicator of birth time. We found that one of the two main sensillum types, the taste papillae, arise according to a clear spatial gradient of birth times, whereas the other sensillum type, taste hairs, arise without any apparent spatial ordering. Within each sensillum type, there was a strong tendency for either all or none of the neurons to have incorporated BrdU. Among those rare sensilla in which only some of the neurons incorporated BrdU, there were clear patterns of the distribution of labeled and unlabeled neurons per sensillum. These results suggest that subsets of the neurons of a sensillum are siblings, and thus argue against the possibility that the several neurons of a sensillum arise from a single stem cell precursor through repeated asymmetrical divisions. © 1995 John Wiley & Sons, Inc. 相似文献
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The blowfly has taste chemosensilla on the labellum. The sensory receptor cells in the chemosensillum are highly specialized for the tastes of sugar, salt and water, respectively. Previously we introduced chromosaponin I (CSI) and glycyrrhizin (GL), as sweet substances for the blowfly, Phormia regina. Application of these triterpenoid saponins induced feeding responses as well as impulses of the sugar taste receptor cell in the LL-type sensillum at a much lower concentration than that of sucrose. In the present paper, we show the involvement of G protein-mediated cascade in the CSI- and GL-responses as well as in sugar responses. CSI activates the sugar signal transduction cascade after penetrating through the membrane. On the other hand, GL exerts dual effects to stimulate the sugar signal transduction possibly by activating it inside the cell and also by interacting with the pyranose sugar receptor site. A non hydrolyzable G protein inhibitor guanosine 5′-O-(2-thiodiphosphate), GDPβS, markedly decreased the responses of the sugar receptor cell to the two triterpenoid saponins as well as the response to sucrose and fructose. These results suggest that CSI and GL are direct activators of G protein. 相似文献
6.
Summary We have traced the central projections of the receptor neurons associated with each of the eleven largest taste hairs on the labellum of the blowfly, Phormia regina (Meigen), by staining them with cobaltous lysine. The eleven hairs fall into three groups which reflect their peripheral locations and their branching patterns in the subesophageal ganglion. Group 1, consisting of the anterior hairs (numbers 1 and 2) and Group 3, consisting of the posterior hairs (numbers 9–11) project bilaterally, while Group 2, consisting of the middle hairs (numbers 3–8) projects primarily ipsilaterally. The central projections of the hairs within a single group are similar. Each hair houses four chemoreceptors, which have differing chemical sensitivities and behavioral roles, and one mechanoreceptor. In some cases, there were indications that the different cells within a single hair have different central branching patterns. For some hairs, however, it was clear that a single central branching region and pattern was shared by more than one receptor cell. We failed to find either a continuous somatotopic representation of a hair's position on the periphery, or an anatomical segregation of receptors coding for different modalities. Behavioral experiments indicate that the fly is informed both of the identity of the hair stimulated and of the chemical nature of the stimulus. Our results suggest that this information is not represented on a gross anatomical level. 相似文献
7.
Adaptation-promoting effect of IP3, Ca2+, and phorbol ester on the sugar taste receptor cell of the blowfly, Phormia regina.
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The fly has a receptor cell highly specialized for the taste of sugars. We introduced inositol 1,4,5-trisphosphate (IP3), Ca2+, or a phorbol ester, 12-deoxyphorbol 13-isobutylate 20-acetate (DPBA), into the cell and investigated their effects on the response to sucrose. The sugar receptor cell generates impulses during constant stimulation with sucrose, but the impulse frequency gradually declines as the cell adapts to the stimulus. Thus, this gradual reduction of the impulse frequency is a direct manifestation of adaptation of the cell. These reagents accelerated the gradual reduction of the impulse frequency, although the initial impulse frequency was little affected. In contrast to these reagents, glycoletherdiamine-tetraacetate (EGTA) retarded the gradual reduction of the impulse frequency. Moreover, when IP3 and DPBA were applied together, the gradual reduction of the impulse frequency was more accelerated than when either IP3 or DPBA was applied. When IP3 and EGTA were applied together, however, the accelerating effect of IP3 tended to be canceled. Based on these results, we hypothesized that an intracellular cascade involving inositol phospholipid hydrolysis, intracellular Ca2+ mobilization, and protein kinase C-mediated phosphorylation promotes adaptation of the sugar receptor cell. 相似文献
8.
Flies provide a powerful model system for exploring signaling systems in gustatory receptor neurons (GRNs). To elucidate the cellular and molecular bases of these signaling systems, we sought to develop techniques to dissociate GRNs. We developed a primary culture of GRNs isolated from the labella of the blowfly, Phormia regina, 4-5 days after pupation. Dissected labella were treated with papain in a low Ca2+ saline solution and shaken in Leibovitz's L-15 medium supplemented with 20-hydroxyecdysone, L-ascorbic acid, and trehalose with a test tube mixer. Released cells were plated and kept at 29 degrees C in a medium containing fetal bovine serum. After a minimum of 2 days in culture, we observed survival or growth of bipolar cells with the characteristic morphology of GRNs. We also examined taste responsiveness by monitoring intracellular Ca2+ with a Ca2+-sensitive fluorescent dye, fluo-3. For some bipolar cells, application of sucrose, NaCl, or LiCl for 5-20 s transiently increased the intracellular Ca2+ levels in cell bodies for 20-30 s. The primary cell culture described here is useful for functional analysis of GRNs. 相似文献
9.
In insects, the functional molecules responsible for the taste system are still obscure. The gene for a 28.5 kDa protein purified from taste sensilla of the blowfly Phormia regina belongs to a gene family that includes takeout of Drosophila melanogaster. Molecular phylogenetic analysis revealed that the Phormia Takeout-like protein is most similar to the protein encoded by a member of the Drosophila takeout gene family, CG14661, whose expression and function have not been identified yet. Western blot analyses revealed that Phormia Takeout-like protein was exclusively expressed in antennae and labellum of the adult blowfly in both sexes. Immunohistochemical experiments demonstrated that Takeout-like protein was localized around the lamella structure of the auxiliary cells and in the sensillar lymph of the labellar taste sensillum. In antennae, Takeout-like protein was distributed at the base of the olfactory sensilla as well. No significant differences in Takeout-like protein expression were found between the sexes. Our results suggest that Phormia Takeout-like protein is involved in some early events concerned with chemoreception in both the taste and olfactory systems. 相似文献
10.
Akio Shiraishi Nobuyuki Miyachi 《Journal of comparative physiology. A, Neuroethology, sensory, neural, and behavioral physiology》1976,110(1):97-109
Summary The proboscis extension reponse of the blowfly during stimulation of the tarsal sugar receptors was inhibited by the presence of NaCl. Acceptance thresholds for sucrose in various concentrations of NaCl were measured. The median acceptance thresholds for sucrose in mixtures of 0.01, 0.25, 0.5 and 1.0 M NaCl were 1.8 × 10–3, 6.0 × 10–3, 1.2 × 10–2, and 2.0 × 10–2 M, respectively. Concentration-response curves for sucrose in the tarsal D-type sugar receptor shifted to the right under the existence of high concentration of NaCl. Number of impulses per D-type sugar receptor at the median acceptance thresholds described above were 7.5, 8.4, 6.8 and 10.4 for the first 0.1 s of stimulation, respectively. The average number was 8.2 impulses per 0.1 s. Comparisons were made between the behavioral acceptance thresholds (1) on one leg exposed to sucrose mixed with 0.01 M NaCl and (2) on two contralateral legs, one of which was exposed to sucrose in 0.01 M NaCl and the other to 0.5 M NaCl alone. The acceptance thresholds from two experiments agreed with each other. The median threshold value was 1.7 × 10–2 M sucrose. Behavioral inhibition by NaCl in mixtures with sucrose can be explained by its peripheral inhibition of sugar receptors.This research was supported in part by ITO foundation and Scientific Research Fund from the Ministry of Education of Japan. 相似文献
11.
The insoluble α-glucosidase activity was examined in three kinds of preparations of the fly labella: labellar integuments with intact chemosensilla, fragments of nerve bundles, and sensilla cut off from the labella.α-Glucosidases having a similar affinity for substrates to isozyme P-II were found in each preparation.Kinetics, inhibition by tris(hydroxymethyl)amino methane (Tris), and pH dependence were examined on the activity of both the membrane-fixed and solublized glucosidase isozymes.Most of the insoluble activity was observed in the nerve bundle fractions, which showed the same properties as P-II in the soluble fraction but were not solubilized by chaotropic anion or by addition of EDTA (insoluble P-II). A different type of insoluble α-glucosidase has been suggested to exist in the labellar chemosensilla in addition to insoluble P-II. It showed a very broad pH dependence and had a larger inhibition constant for Tris. It was inferred to be identical with the enzyme found at the tip of the intact chemosensilla. The hypothesis that the above isozyme might be the pyranose site of the sugar receptor was supported. 相似文献
12.
Ayami Matsushima Satoru Yokotani Xiaohui Lui Kazunori Sumida Takeshi Honda Seiji Sato Atsushi Kaneki Yukimasa Takeda Yoshiro Chuman Mamiko Ozaki Daisuke Asai Takeru Nose Hitoshi Onoue Yushi Ito Yoshiya Tominaga Yasuyuki Shimohigashi Miki Shimohigashi 《International journal of peptide research and therapeutics》2003,10(5-6):419-430
Pigment-dispersing factor PDF is an 18-amino acid insect neuropeptide that mediates a circadian rhythmicity in locomotor activity.
PDF is coded in a precursor protein together with another neuropeptide named PDF-associated peptide, PAP. PDF is highly conserved
among insects, whereas the homology of PAPs is very low with considerably varied amino acid sequences. Since such dissimilarity
has suggested that the function of PAP peptide is not associated with that of PDF, we have attempted to analyze the sequences
of PDF precursor proteins among a series of species of insects and hypothesized that PDF precursors are classified into at
least three different classes: Drosophila-Musca, Meimuna-Romalea, and Gryllus. In order to exemplify this hypothesis, we here describe the molecular cloning of the pdf-gene of the black blowfly Phormia regina and an in silico screening for the pdf-gene in the genome databank of the mosquito Anopheles gambie, both species belonging to the Diptera. It was found that deduced amino acid sequences of PDF peptides are almost completely
conserved among all Dipterans and also the amino acid sequences of PAPs are considerably highly preserved (55–82 similarity)
among the species of Diptera. The results confirmed the validity of grouping the PDF precursor proteins. In situ hybridization was carried out in fly brains to identify the precise locations of pdf-expressing cells and to examine any daily cycling of pdf mRNA. Intense signals for pdf mRNA were identified in the medulla, but not in the pars lateralis where PDF neurons were strongly immunostained by the antibody
raised against PDF peptide. Hybridization was also performed for the brain samples at two hour intervals throughout the day.
Although very intense hybridization signals were observed at ZT8 even in some neurites, no prominent rhythmicity of pdf mRNA expression was observed. 相似文献
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A M Angioy A Liscia P Pietra 《Bollettino della Società italiana di biologia sperimentale》1979,55(20):2100-2104
The electrical resistance of the labellar taste hairs of Phormia regina M. is enhanced by aging in both males and females; this increase is greater in males. Resistance variations, related to changes in response of stimulated chemoreceptors, may explain, at least in part, the reduction in reflex food intake, O2 consumption, weight increase and, on the whole, survival capabilities of males vis-à-vis female insects. 相似文献
15.
ABSTRACT. Incubation conditions have been established for the corpus cardiacum-corpus allatum (CC-CA) complex of female Phormia regina (Meigen), which will support CC-CA biosynthetic activities in vitro as measured by the incorporation of a labelled methyl group with L-[methyl-3 H]methionine as the methyl donor. After incubation, radioactivity in the organic extract of the medium was determined by scintillation counting. Analysis of the organic extract with reverse phase high-performance liquid chromatography (HPLC) revealed that a compound which has similar retention time (UV absorbance) with synthetic JH III was synthesized by the CC-CA complexes of liver-fed females. By using this short-term, in vitro , radiochemical assay for CA activity, it was shown that a protein diet significantly increases the activity of the CA compared with females fed only a sugar-water diet. Furthermore, use of HPLC separation, in conjunction with scintillation counting of time-collected fractions, demonstrated the existence of a moiety containing incorporated radiolabeled methyl group (from the methionine) which did not co-elute with JH I or JH III standards. These results suggest that in P. regina use of the incorporation of a radiolabeled methyl group to measure JH biosynthesis (CA activity) can be misleading if the compounds which do not co-elute with JHs are not considered. 相似文献
16.
Summary The development of the sensory neurons of the legs of the blowfly,Phormia regina has been described from the third instar larva to the late pupa using immunohistochemical staining. The leg discs of the third instar larva contain 8 neurons of which 5 come to lie in the fifth tarsomere of the developing leg. Whereas 2 neurons persist at least to the late pupa, the other cells degenerate. The first neurons of gustatory sensilla arise in the fifth tarsomere at about 1.5 h after formation of the puparium. Most of these sensilla, however, appear within a short time period beginning at about 18 h. The femoral chordotonal sensory neurons first appear at the time of formation of the puparium, as a mass of cells situated in the distal femur. During later pupal development 2 groups of these cells come to lie at the femur-trochanter border, where they become the proximal femoral chordotonal organ of the adult; the remaining cells become the distal femoral chordotonal organ. Other scolopidial neurons appear later in development. The nerve pathways of the late pupal leg are established either by the axons of the cells that are present in the larval leg disc or by new outgrowing processes of sensory neurons. In the tibia, the initial direction of new outgrowth differs in different regions of the segment: proximal tibial neurons grow distally, while distal tibial neurons grow initially proximally. 相似文献
17.
Toxicity and behavioural effects of diet-borne alkaloids on larvae of the black blowfly,Phormia regina 总被引:2,自引:0,他引:2
Larvae of the black blowfly, Phormia regina (Meigen) (Diptera: Calliphoridae) were exposed for 24 h to artificial diets that contained one of the following alkaloids: arecoline, caffeine, nicotine, quinine, sparteine or strychnine at either 1000 or 100 p.p.m. Each of the alkaloids caused reduced weight gain, relative to a control population in a no-choice bioassay and, with the exception of quinine, all alkaloids caused reduced larval weights in a choice bioassay. Larvae were unable to move away from diets containing arecoline (1000 and 100 p.p.m) and congregated away from diets containing 1000 p.p.m. quinine. Arecoline (1000 p.p.m) and both concentrations of nicotine caused significant mortality of larvae. Over a longer period (120 h), 10 and 1 p.p.m. nicotine resulted in significant numbers of larvae congregating away from a treated diet. Ten p.p.m. nicotine caused reduced weight gain over 120 h, although larvae provided with a choice were less affected. Exposure of larvae to dried residues of nicotine for 2 h did not affect subsequent development. 相似文献
18.
- 1 Neurosecretary cells in the central nervous system of the adult blowfly, Phormia regina Meig., have been examined histologically using the parparaldehyde-fuchsin and Gomori's staining method. Six groups of the neurosecretory cells occur in each hemisphere of the brain, the medial, frontal, lateral A, lateral B, posterior I and posterior II groups. In the subesophageal ganglion, four B-cells and two A-cells are present. In the thoracico-abdominal ganglion, ten A-cells are found in the thoracic region and a total of about 50 A- and B-cells in the hind part of the abdominal region.
- 2 A comparison with the neurosecretory system of two other species of blowfly, Calliphora erythrocephala Meig., Sarcophaga bullata Parker, and the housefly, Musca domestica L., showed similar arrangements and grouping.
- 3 Neurosecretory granules have been observed along the axons originating from the medial neurosecretory cells of the brain, and the thoracico-abdominal ganglion. The granules originating from the medial groups can be traced directly to the corpus cardiacum from which they move to the aorta, crop duct and cardia through axons.
- 4 There is with advancing age a gradual increase in the size of cell bodies and nuclei of the median neurosecretory cells in both females and males of Phormia regina, and also a decrease in stainable granules. This increase in size is dependent on nutrition, with no increase in water alone, a slight increase on sugar, and a maximum increase on sugar and liver. Corresponding increases in size occur in the ovaries in connection with feeding the same substances.
19.
Oogenesis in most adult insects is a nutrient-dependent process involving ingestion of both proteins and carbohydrates that ultimately depends on peripheral input from chemoreceptors.The main goal of this study was to characterize, in the female blowfly Phormia regina, the responsive changes of the labellar chemoreceptors to carbohydrates and proteins in relation to four different stages along the ovarian cycle: (1) immature ovaries, (2) mid-mature ovaries, (3) mature ovaries and ready for egg-laying and (4) post egg-laying ovaries. Then, the possible effects exerted by exogenous serotonin on the chemoreceptor sensitivity profiles were investigated.Our results show that ovary length, width and contraction rate progressively increase from stage 1 to 3, when all these parameters reach their maximum values, before declining in the next stage 4.The sensitivity of the labellar “sugar” chemoreceptors to both sucrose and proteins varies during the ovarian maturation stages, reaching a minimum for sucrose in stage 3, while that to proteins begins. Exogenous 5-HT supply specifically increases the chemoreceptor sensitivity to sugar at the stages 3 and 4, while it does not affect that to proteins.In conclusion, our results provide evidence that in female blowflies the cyclic variations in the sensitivity of the labellar chemosensilla to sugars and proteins are time-related to ovarian development and that during the stages 3 and 4 the responsiveness of the sugar cell to sucrose is under serotonergic control. 相似文献
20.
Hormonal regulation of trehalose metabolism in the blowfly, Phormia regina: interaction between hypertrehalosemic and hypotrehalosemic hormones 总被引:1,自引:0,他引:1
Hypertrehalosemia occurs two days after cardiacectomy of adult male Phormia regina with no attendant change in fat body glycogen. In spite of this, cardiacectomized flies caused to fly for 10 min show a lower rate of haemolymph trehalose turnover, and seem to have a decreased capability for synthesizing trehalose from haemolymph glucose. Phormia brain is shown to contain a hypotrehalosemic hormone whose release depends on the integrity of the stomatogastric nervous system. It is possible that the hypertrehalosemic condition in cardiacectomized flies is a result of the absence of this hormone from the blood. 相似文献