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1.
Incubation of abbreviated tRNA's (tRNA-C-COH's) with (chemically) preaminoacylated P1, P2-di(adenosine 5'-)diphosphates in the presence of purified RNA ligase effected transfer of an aminoacyladenylate moiety to the 3'-terminus of the abbreviated tRNA's in good yield. Aminoacylated (or misacylated) tRNA's may thus be prepared from fractionated or unfractionated tRNA-C-COH's; each of the five aminoacylated dinucleoside diphosphates tested was utilized as a substrate by RNA ligase. That the resulting "chemically aminoacylated" tRNA's were identical with those prepared by enzymatic aminoacylation was judged by comparison of 1) chromatographic properties on benzolated diethylaminoethyl-cellulose, 2) rates of chemical deacylation, and 3) affinities for elongation factor Tu, as well as 4) the ability of misacylated tRNA's so derived to be deacylated chemically and then reactivated enzymatically with their cognate amino acids. 相似文献
2.
G V Maksimov A A Churin V Z Paschenko A B Rubin 《General physiology and biophysics》1990,9(4):353-360
Raman spectroscopy (RS) study shows that the "potential sensor" responds to changes in intramembrane potential by conformational changes. The mechanism of regulation of the channel "gate" by the carotenoid potential sensor is discussed. 相似文献
3.
Summary We confirm the hypothesis of Low (1973) that many E. coli K 12 strains contain a prophage (the Rac prophage) located a few minutes clockwise of the trp operon on the genetic map. We have used restriction endonucleases and 32P-labelled probes to construct a physical map of this prophage. Some E. coli K 12 strains, including AB1157, have lost the entire prophage, apparently by a specific deletion. This is consistent with prophage excision by site-specific recombination. reverse (rev) phages (Zissler et al., 1971) are recombination proficient derivatives of phage in which the phage recombination functions have been replaced by analogous functions (RecE) derived from the host chromosome (Gottesman et al., 1974; Gillen et al., 1977). Our data support the origin of rev phages by recombination between and the Rac prophage following excision of the Rac prophage from the E. coli chromosome.Important experimental data are included in the Figure legends. 相似文献
4.
Hapten-specific IgE antibody responses in mice. VII. Conversion of IgE "non-responder" strains to IgE "responders" by elimination of suppressor T cell activity. 总被引:16,自引:0,他引:16
Mice of the inbred strains SJL (H-2s) and AKR (H-2k) are "non-responders" and "low-responders," respectively, in terms of their capacity to develop antibody responses of the IgE class when immunized with conventional proteins and hapten-protein conjugates under conditions optimal for eliciting IgE responses in "high-responder" mice, such as BALB/c (H-2d), to these same antigens. For example, BALB/c mice preimmunized with ASC and then challenged 7 days later with DNP-ASC develop peak augmented primary IgE anti-DNP antibody responses of 320 PCA units, whereas SJL and AKR mice develop responses which are 16-fold and 4-fold lower, respectively. However, pretreatment of the latter two strains with appropriate doses of either x-irradiation (150 R), cyclophosphamide (100 mg/kg) or ALS (150 mul) before carrier-preimmunization strikingly enhances the magnitude of IgE antibody responses in such mice to levels as high as 64-fold above those of untreated control mice of the same strains. Evidence obtained in these experiments indicates that the capacity of such maneuvers to to convert poor IgE responders to high responder status reflects elimination of nonantigen-specific suppressor T lymphocytes which are naturally present and normally function to suppress or "dampen" the IgE antibody response in a relatively selective manner. It appears that these cells modulate IgE responses by acting at least at two distinct points: 1) The most effective activity seems to be at the level of induction of carrier-specific helper T cells; 2) A second locus of inhibitory activity is more distal in the response, either impeding helper T cell-B cell cooperative interactions or suppressing B cell differentiation and/or function directly. Taken collectively, these observations demonstrate that the state of poor responsiveness of the SJL and AKR strains for the IgE antibody class is not a reflection of a genetic inability to develop IgE responses but rather a manifestation of a genetic capability to actively inhibit IgE antibody synthesis. 相似文献
5.
D C Birdsell G E Gavelin G M Kemsley K S Hein 《Plastic and reconstructive surgery》1981,68(5):742-745
An experiment was designed to compare the "holding power" or "staying power" of absorbable (polyglycolic acid and polyglactin 910) and nonabsorbable (nylon) suture. The aim of this experiment was to determine what provides the lasting strength of the bond between soft tissues that are approximated or plicated. When correcting the rectus diastasis during abdominoplasty, we used nylon sutures in 15 patients and absorbable synthetic sutures in 15 other patients. We then marked the closed folds of the rectus sheath with small metal vascular clips. Two days later and approximately 6 months after operation an upright anteroposterior abdominal x-ray was taken and the position of the metal clips was compared in the test groups. Although there was usually slight separation of the clips after 6 months, no significant difference between the two groups was noted, thereby indicating that holding power is not related to type of suture material but more likely to fibroplasia. 相似文献
6.
The red eye pigment of Drosophila melanogaster consists of six complex pteridines known as neodrosopterin, drosopterin, isodrosopterin, fraction e, and aurodrosopterins (2); these pigments are greatly reduced in the purple mutant. Conditions for biosynthesis of these "drosopterins" are described and compared with those for the synthesis of sepiapterin. The enzymes are contained in a soluble, pteridine-free extract obtained between 40 and 60% saturated ammonium sulfate. The results indicate that sepiapterin synthase consists of two enzymes, the first of which provides a precursor for "drosopterin" biosynthesis. The evidence is (1) the purple mutant, low in accumulated sepiapterin and "drosopterins", is known to have approximately 10% of the sepiapterin synthase activity of wild type; (2) unlabeled sepiapterin does not cause isotope dilution of "drosopterin" synthesis; (3) the 600g pellet prepared from a wild-type head homogenate contains "drosopterin" synthesizing activity and no sepiapterin synthase, yet a heat-labile factor in this fraction stimulates sepiapterin synthesis in the 100000g supernatant of wild-type or pr flies; (4) sepiapterin and "drosopterin" syntheses require Mg2+; (5) sepiapterin synthesis is stimulated by NADPH; "drosopterin" synthesis responds to either NADPH or NADH. Although "drosopterins" are complex pteridine-type pigments, we have demonstrated their biosynthesis by soluble enzymes. This allows us to consider investigation into the mechanism by which the amounts of these pigments are regulated. 相似文献
7.
V Brabec 《Folia haematologica (Leipzig, Germany : 1928)》1976,103(4):552-558
The values of haemoglobin, reticulocytes and half-times of the filtrability of non-washed and washed erythrocytes were examined in male albino rats, Wistar strain, after i.p. injections of methylcellulose (MC) and compared with controls. In individual experiments the rats received 2 to 32 injections of MC. In injected animals, the filtrability of non-washed erythrocytes was altered. The filtrability half-times of the washed erythrocytes did not differ from the controls. Thus, the filtrability is altered for extracorpuscular reasons. "Hypersplenism" being completely developed, (after 32 MC injections), the filtrability of non-washed erythrocytes repaired when the application of MC had been discontinued, the reticulocyte values remained however increased. Problems of the mechanism of anaemia in experimental "hypersplenism" after MC injections in rats and relations between the altered filtrability of the erythrocytes and the haemolysis are discussed. 相似文献
8.
Shnol' SE Zenchenko KI Berulis II Udal'tsova NV Zhirkov SS Rubinshteĭn IA 《Biofizika》2004,49(1):132-139
It was shown in experiments with collimators that the changes with time in the shape of histograms constructed from the measurements of the alpha-activity of 239Pu microsamples depend on the direction of the flow alpha-particles. If the flow of alpha-particles was directed to the Polar Star, the dependence of the probability of the realization of histograms of similar shape on time disappeared. The measurements of alpha-particle flows directed to the East and West revealed a high probability of the repeated realization of histograms of similar shape in 1436 min, i.e., in a sederal day. A comparison of "west" and "east" histograms showed a high probability of the realization of similar "west" histograms 718 min (half a sideral day) later than "east" histograms. The results suggest that the shape of histograms depends on the pattern of the starry sky and the space-time inhomogeneity corresponding to this pattern. 相似文献
9.
F Bruschi 《Parassitologia》1992,34(1-3):23-30
Many helminth infections have a "lung phase" whose significance considering the host-parasite relations has not yet been clarified. In some nematode infections larvae must pass through the lungs, in others adult worms live in the respiratory tract, and some others cause tropical pulmonary eosinophilia. Some examples of these infections (Nippostrongylus brasiliensis, Schistosoma mansoni, Trichinella spiralis and Toxocara canis) are considered to clarify whether this phase of infection is a "pro-host" or a "pro-parasite" mechanism. Whereas in primary infections--in some cases--transit through the lungs may render the parasite more resistant to host defence mechanisms (e.g., Schistosoma and perhaps Trichinella infections) or enables completion of the cycle (Nippostrongylus and Toxocara infections), in secondary infections the lungs might be the site of parasite killing. 相似文献
10.
“三料”转化与生态农业 总被引:2,自引:0,他引:2
农业生态经济系统中,饲料、肥料、燃料作为能流、物流的主要形态和通道,影响着整个系统的结构和功能。本文用源、流、库的关系和数据资料,分析了“三料”转化的重要作用。提出了如何提高其转化效率,促进生态农业建设的技术路线。秸秆资源和腐食食物链的开发是技术关键。 相似文献
11.
Biopsy specimens from 38 "de-epithelized" dermal pedicles were examined microscopically. There was considerable variation in the depth of the plane among surgeons, procedures, and even between specimens from two sides of a bilateral procedure done by the same surgeon. Usually, the "de-epithelization" removed all the epidermis plus the upper layer of dermis containing the pilosebaceous apparatus. The significance of this finding as related to the future development of epidermal inclusion cysts is uncertain. Also, it brings into question the importance of "the dermal plexus circulation," which many have thought to be critical for viability of the nipple. 相似文献
12.
D Wender 《BMJ (Clinical research ed.)》1987,295(6613):1595-1597
For a stroke victim there may be at least three types of strange occurrences: incorrect saying, seeing, and thinking. To the patient only the third seems to be "crazy". After a stroke (left hemisphere), which mainly produced serious aphasia, I (the patient) felt crazy two or three times when someone said something I expected him to say. On the other hand, my initial aphasic "gibberish speech" and an occasional false vision did not seem crazy. In my case the vision is always a car or a child, seen on my extreme right, where I am otherwise blind from the stroke. I am always driving when it happens; in recent years this phenomenon occurs when I am tired or tense, or the light is poor. These rapid visions do not seem insane but merely physical problems in my eyes, much like ordinary people''s dreams. 相似文献
13.
D K Ford 《Journal of bacteriology》1962,84(5):1028-1034
Ford, Denys K. (University of British Columbia, Vancouver, Canada). Culture of human genital "T-strain" pleuropneumonia-like organisms. J. Bacteriol. 84:1028-1034. 1962.-The conditions under which "T-strain" pleuropneumonia-like organisms, as described by Shepard, are best cultured were investigated. The organisms were found to grow on several types of nutrient agar and broth, of which PPLO medium supplemented with yeast extract and horse serum was the simplest. Subculture was possible through broth cultures, provided the broths were not incubated longer than 16 hr. The organisms on agar required either Fortner's anaerobic atmosphere or 10% CO(2), but broth cultures grew aerobically. "T-strains" grew over a pH range of 6.8 to 7.8, and a temperature range of 30 to 36 C. They were viable after storage for 16 days at 4 C and for 90 days at -20 C, and they resisted lyophilization. They were sensitive to 1.5 mug per ml of tetracycline and streptomycin, but were resistant to ampicillin and penicillin. Quantitative studies showed maximal concentration in broth of 10(6) to 10(7) organisms per ml, and logarithmic multiplication for the first 12 hr of broth culture, with a subsequent rapid decline in number. Colonial morphology was maintained after numerous subcultures. 相似文献
14.
Summary The mitochondrial ATPase from a PHO 1 mutant (OLI 2, PHO 1, OLI 4 region on mit DNA of S. cerevisiae) was further examined. A new purification method using Lysolecithin instead of Triton allowed us to solubilize and separate a heterogeneous ATPase population from PHO 1-mitochondria: the major abnormal fraction had extremely low oligomycin-sensitivity (but normal specific immunological reactivity), while a minor normal fraction (representing about 20% of the initial mitochondrial ATPase activity) had high sensitivity and affinity for oligomycin.Moderate urea treatment of PHO 1-mitochondria leads to partial loss of ATPase activity and a concomitant increase of oligomycin-sensitivity, suggesting that a heterogeneous ATPase population exists in situ in the mitochondrial membrane: part of the major abnormal ATPase fraction is selectively inactivated by urea, producing a concomitant enrichment in the initially minor normal ATPase fraction.If the minor normal ATPase fraction is the only one capable of in vivo ATP synthesis, the deficient but oligomycin-sensitive cell growth and oxidative phosphorylation in vitro are readily explained.Further structural studies are under way to ascertain whether the minor normal ATPase fraction is strictly identical to the wild type, in which case PHO 1 is a regulatory gene, or not, in which case PHO 1 is a structural gene. 相似文献
15.
S V Kochetkova T L Tsilevich I P Smirnov I L Shchaveleva A A Khorlin 《Bioorganicheskaia khimiia》1988,14(6):820-823
"Full" acyclic analogues of ribonucleosides, 1-(1,2,6-trihydroxy-4-oxahex-3-yl)thymine and -cytosine, 3- and 9-(1,2,6-trihydroxy-4-oxahex-3-yl)adenine, and 9-(1,2,6-trihydroxy-4-oxahex-3-yl)guanine, have been prepared by condensation of silylated nucleo-bases with 1,4,5,6-tetraacetoxy-3-oxahexane in the presence of Lewis acids followed by deacetylation. 相似文献
16.
Hoyt JD 《Bioethics quarterly》1981,3(2):128-132
A friend of Sharon Siebert's describes her efforts leading to the issuing of a restraining order (Hoyt v. St. Mary's Rehabilitation Center) to prevent the nursing home from implementing a "do not resuscitate" order written by the physician of the brain-damaged Siebert. 相似文献
17.
B A Young 《The Journal of experimental zoology》1991,260(3):275-287
A bioacoustic analysis is conducted on the defensive sounds produced by 21 species of snakes. The "typical" snake hiss is described as having a broad-frequency span (from roughly 3,000 to 13,000 Hertz) and a dominant frequency near 7,500 Hertz. The "growl" of the king cobra (Ophiophagus hannah) differs from the "typical" snake hiss in consisting solely of frequencies below 2,500 Hertz, with a dominant frequency near 600 Hertz. Structural analysis of the upper respiratory tract of O. hannah suggests that the "growl" is produced by tracheal diverticula functioning as low-frequency resonating chambers. This hypothesis is supported in several ways. An acoustic analysis of a mechanical model of the trachea demonstrates the potential for these diverticula to produce resonance effects. A "growl" also occurs in the mangrove ratsnake (Gonyosoma oxycephalum), a species that also has tracheal diverticula. Flushing the respiratory tract of G. oxycephalum with helium produces a shift of over 1,000 Hertz in the "growl," a shift that is indicative of a resonance effect. 相似文献
18.
An animal model was developed to determine the feasibility of vascularizing a porous biomaterial and transferring it as part of a free flap to a recipient site with enhanced resistance to infection due to the maintenance of a blood supply. To that end, the experiment was divided into three stages. Stage 1 compared the soft-tissue response of Interpore-200 to Interpore-500, both continuously porous hydroxyapatite materials implanted beneath the panniculus carnosus of the male Sprague-Dawley rat. Pore size was an important factor as it influenced vascular ingrowth, with Interpore-200 vascularizing earlier (complete at 1 week) and more intensely. Interpore-200 was therefore used for the remainder of the experiment. After 1 week of tissue ingrowth, the implants were moved from the abdomen to the skull on a vascular pedicle as a "biocomposite" free flap. Stage 2 was the histologic evaluation of 15 "biocomposite" free flaps over various time intervals up to 8 months. The free flaps formed a fibrous union to the skull, while a simple nonvascularized Interpore-200 onlay graft (stage 2 control) demonstrated a bony union in three of four implants placed up to 2 months. Stage 3 confirmed the free flap's resistance to bacterial infection. A highly significant difference (p less than 0.005) in infection rates was demonstrated between the "biocomposite" and nonvascularized stage 3 controls with no Pseudomonas growth from 9 of 10 cultures of the free flaps 5 days after exposure to 10(4) Pseudomonas aeruginosa, while stage 3 controls demonstrated Pseudomonas growth in all cultures (heavy growth in 8 of 10). The "biocomposite" free flap has excellent potential to provide form and structure to wounds requiring reconstruction where bacterial contamination is a significant risk factor. 相似文献
19.
C L Brooks 《Journal of molecular biology》1992,227(2):375-380
We have used molecular dynamics simulation methods to study the structure and fluctuations of "native" apomyoglobin in aqueous solution for a period of greater than 0.5 nanosecond. This work was motivated by the recent attempts of Hughson et al. to characterize the structure and motion of both this molecule and the less compact, acid stabilized I stage, using methods of pulsed H/2H exchange. The study of these systems provides new insights into protein folding intermediates and our simulation has yielded a detailed model for structure and fluctuations in apomyoglobin which complements the experimental studies. We find that local (short-time) fluctuations agree well with fluctuations observed for the holoprotein in aqueous solution, as well as results from the crystallographic B-factors. In addition, the structural features we observe for native apomyoglobin are very similar to the holoprotein, in basic agreement with the findings of Hughson et al. By examining larger-scale motions, developing only over timescales in excess of a 100 picoseconds, we are able to identify conformationally "labile" and "non-labile" regions within native apomyoglobin. These regions correspond extremely well to those identified in the nuclear magnetic resonance experiments as unstable and stable "folding subdomains" in the I state of apomyoglobin. Overall we find that helices A, B, E, G and H show the least amount of motion and helices C, D and F move substantially over the timescales examined. The major motions, and the primary difference between the holo and apo structures as we have observed them, are due to the shifting motion of helices C, D and F into the vacant heme cavity. We also find that motions at the interface of helical segments can be large, with one important exception being the chain segment connecting helices G and H. This segment of chain interacts with the conformationally "non-labile" helix A to form a relatively rigid subdomain composed of helices A, G and H. We believe that these findings provide direct support for the suggestion of Hughson et al. that helices A, G and H constitute a compact subdomain that remains in a native-like conformation as the protein begins to unfold in environments of decreasing pH. 相似文献
20.
Microsurgical forearm "cricket bat-transformer" phalloplasty. 总被引:1,自引:0,他引:1
D A Gilbert G H Jordan C J Devine B H Winslow 《Plastic and reconstructive surgery》1992,90(4):711-716
Presently, the donor flap of choice for microsurgical phallic reconstruction is the radial forearm flap. The success of several different design modifications confirms the reliability of the radial and ulnar forearm flaps. Farrow et al. described their experience with the "cricket bat" concept in 1980. To the previous "cricket bat" design, we now wish to add modifications. These modifications utilize longitudinal and transverse rotations of the linear forearm tissues to create a phallus--much like the transformation of a toy robot into a truck. Deepithelialized flaps and a full-thickness skin graft coronoplasty complete glans reconstruction. The "cricket bat-transformer" flap appears to produce the most predictable results in subtotal phallic reconstructions and phallic constructions in the pediatric and transgender patient groups. 相似文献