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1.
The addition of the divalent cation ionophore A23187, carbachol, norepinephrine or insulin to rat fat cells elevated cyclic GMP. The increase in cyclic GMP due to these agents was greater at 4 than at 2 minutes after their addition. Cyclic GMP accumulation was also elevated by the addition of 0.1 to 0.5 mM sodium oleate in the presence of 0.1% albumin and by albumin containing added palmitate with an FFA/albumin molar ratio of 6.7. The rise in cyclic GMP due to all agents was markedly reduced in calcium-free buffer. The effects of the various agents on cyclic GMP accumulation in rat fat cells had little correlation with lipolysis. Insulin was an effective anti-lipolytic agent in both the presence and absence of calcium while neither A23187 nor carbachol had any effect on fat cell lipolysis. 相似文献
2.
Meiotic maturation was induced in Xenopus laevis oocytes when the external Ca++ or Mg++ ion concentration was raised above 5 mM in the presence of the ionophore. Ionophore-divalent cation-induced maturation appears to be due to the stimulation of the oocyte itself. Cytoplasm of responding oocytes induced maturation when microinjected into ovarian oocytes. Cycloheximid, an inhibitor of progesterone-induced maturation, inhibited the maturational response induced by the ionophore and divalent cations. Ethidium bromide, an inhibitor of the follicular response to human chorionic gonadotropin, had no effect. The possible roles that Ca++ and Mg++ may play in the initiation of maturation are discussed. 相似文献
3.
1. The prior addition of non-aggregating concentrations of the divalent cation ionophore, A-23187, causes human platelets to aggregate in response to a subsequent addition of the 2',3'-dialdehyde and 2',3'-dialcohol derivatives of ADP (oADP and or ADP). Previous studies [Pearce et al. (1978) Eur. J. Biochem. 88, 543--555] have shown that these derivatives act as partial agonists at the platelet ADP receptor inducing only the transition from discoid to globular morphology ('shape change'). A secretion response is also observed on addition of a low concentration of ionophore A-23187 prior to orADP. These responses are not observed if ionophore A-23187 is added prior to the 2',3'-dialdehyde and 2',3'-dialcohol derivatives of ATP (oATP and or ATP) and are markedly inhibited by prior addition of the ADP antagonist, adenosine 5'-[beta, gamma-methylene]triphosphate. 2. The aggregation response to oADP in the presence of ionophore A-23187 is reduced but not eliminated by addition of 3 mM EGTA when studies are performed in heparinised platelet-rich plasma. Additions of 3 mM EGTA in citrated platelet-rich plasma, or of 4 mM EDTA in either system completely inhibits this response. Inhibitors which are reported to elevate the intracellular concentration of adenosine 3':5'-monophosphate (cyclic AMP) or to prevent Ca2+ movement also inhibit the aggregation response to oADP which is observed in the presence of ionophore A-23187. 3. Prior addition of inhibitors of adenylate cyclase fails to cause an aggregation response to subsequent addition of oADP or orADP. Certain of these inhibitors enhance and prolong the shape change response to oADP or orADP but only at concentrations an order of magnitude in excess of those required to antagonise inhibition by agents such as prostaglandin E1, which act by increasing the concentration of cyclic AMP. 4. The concentration of prostaglandin E1, adenosine or papaverine required to inhibit shape change induced by oADP is one to two orders of magnitude lower than that required to inhibit shape change induced by ADP. 5. Prior addition of oADP decreases the lag phase in the response of human platelets to arachidonate while also increasing the concentration required to observe half-maximal response, and causing a decrease in the extent of the response. Prior addition of oATP also diminishes the extent of this response and increases the concentration of arachidonate required but has no effect on the lag phase. 6. The data suggest that oADP and orADP are capable only of acting as partial agonists at the ADP receptor because of a defective ability to increase cytosolic Ca2+ concentration. The defect is rectified by the presence of low concentrations of ionophore A-23187, which promotes mobilisation of Ca2+ from an intracellular store. The results do not appear consistent with the thesis that a decrease in platelet cyclic AMP is an initiating event in aggregation induced by ADP, but do support a model which implicates cyclic AMP in depletion of cytosolic Ca2+. 相似文献
4.
Mobilization of intracellular calcium by extracellular ATP and by calcium ionophores in the Ehrlich ascites-tumour cell 总被引:2,自引:0,他引:2
We have studied the changes of the intracellular free calcium concentration ([Ca2+]i) effected by external ATP, which induces formation of inositol trisphosphate, and by the divalent cation ionophores ionomycin and A23187. Both, ATP (40 microM) and ionophores (1-80 mumol/l cells ionomycin; 20-400 mumol/l cells A23187), produced a transient rise of [Ca2+]i which reached its maximum within 15-30 s and declined near resting values (about 200 nM) within 1-3 min. When the [Ca2+]i peak surpassed 500 nM a transient cell shrinkage due to simultaneous activation of Ca2+-dependent K+ and Cl- channels was also observed. The cell response was similar in medium containing 1 mM Ca2+ and in Ca2+-free medium, suggesting that the Ca mobilized to the cytosol comes preferently from the intracellular stores. Treatment with low doses of ionophore (1 mumol/l cells for ionomycin; 20 mumol/l cells for A23187) depressed the response to a subsequent treatment, either with ionophore or with ATP. Treatment with ATP did also inhibit the subsequent response to ionophore, but in this case the inhibition was dependent on time, the stronger the shorter the interval between both treatments. This result suggests that the permeabilization of Ca stores by ATP is transient and that Ca can be taken up again by the intracellular stores. Refill was most efficient when Ca2+ was present in the incubation medium. Addition of either ATP or ionomycin (1-25 mumol/l cells) to cells incubated in medium containing 1 mM Ca2+ decreased drastically the total cell Ca content during the following 3 min of incubation. In the case of ATP the total cell levels of Ca returned to the initial values after 7-15 min, whereas in the case of the ionophore they remained decreased during the whole incubation period. These results indicate that Ca released from the intracellular stores by either ATP or ionophores is quickly extruded by active mechanisms located at the plasma membrane. They also suggest that, under the conditions studied here, with 1 mM Ca2+ outside, the Ca-mobilizing effect of ionophores is stronger in endomembranes than in the plasma membrane. 相似文献
5.
Resumption of meiotic maturation was induced in follicle-enclosed rat-oocytes by treatment with the divalent cationophore A23187 (10(-5)M). However, the same effect was attained by incubation in Ca++-deficient medium, even in the presence of EDTA or EGTA (1mM). The stability of the first polar body was increased under Ca++-deficient conditions. Neither the ionophore nor Ca++-deficient medium interfered with the spontaneous maturation of isolated oocytes of the rat. The experiments with cultured follicles suggest that redistribution of divalent cations may participate in the physiological control of meiosis in mammalian oocytes. 相似文献
6.
Cytoplasmic activation of starfish oocytes by sperm and divalent ionophore A-23187 总被引:3,自引:2,他引:1
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《The Journal of cell biology》1975,66(1):86-94
The relationship between onset of the early cytoplasmic stages of oocyte activation (vitelline membrane separation and elevation) and nuclear meiotic maturation was investigated in starfish oocytes after their exposure to divalent ionophore (A-23187) or sperm. Meiotically mature oocytes, isolated in calcium-free seawater, underwent activation in response to sperm or ionophore as previously reported. Large, immature starfish oocytes, arrested in prophase I of meiosis (germinal vesicle stage), underwent vitelline membrane elevation when treated with divalent ionophore A-23187 or starfish sperm. Histological studies demonstrated that cortical granule breakdown in the oocyte cortex was associated with vitelline membrane elevation after these treatments. Activation of oocytes by sperm occurred only in response to starfish sperm. Sea urchin, sand dollar, surf clam, or marine worm sperm did not induce vitelline membrane elevation of either immature or mature starfish oocytes. Sperm- or ionophore-activated immature oocytes underwent nuclear maturation after addition of the meiosis-inducing hormone, l-methyladenine; however, parthenogenetic development did not occur and embryonic development was markedly inhibited. In contrast to previous studies, the present results indicate that cytoplasmic activation can be initiated before and without hormone induction of the nuclear maturation process. Differentiation of the oocyte cell surface or cortex reactivity therefore appears to occur during oogenesis rather than as a consequence of maturation. The data further support the view that divalent ions mediate certain of the early activation responses initiated by sperm at the time of fertilization and that synchronization of fertilization to the meiotic process in the oocyte is important for the occurrence of normal development. 相似文献
7.
C B Wollheim B Blondel P A Trueheart A E Renold G W Sharp 《The Journal of biological chemistry》1975,250(4):1354-1360
The role of Ca2+ on insulin release has been studied by the use of ionophore A23187. The ionophore complexes divalent cations and permits Ca2+ entry into cells by acting as a carrier in the plasma membranes. Cultured cells obtained by enzymatic digestion of pancreases from newborn rats were studied on the 3rd day of culture. With Ca2+ in the incubation medium the ionophore induced sustained insulin release even in the absence of glucose. Optimal effects of the ionophore were observed at 3 and 10 mug per ml in the presence of 0.3 to 1.0 mM Ca-2+. Under these conditions the insulin release was greater than that caused by 16.7 mM glucose. A graded response was observed to changes in Ca-2+ concentration from 0.1 to 1.0 mM Ca-2+. Higher Ca-2+ concentrations caused a large amount of insulin to be released promptly, but the release was not sustained. Mg-2+ and Sr-2+ were not found to substitute for Ca-2+. Ba-2+ at 0.3 mM stimulated insulin release even in the absence of ionophore. Cyclic adenosine 3':5'-monophosphate was able to increase ionophore-induced insulin release. The alpha-adrenergic effect of epinephrine to inhibit insulin release was not observed in the presence of Ca-2+ and the ionophore, and a stimulatory effect of epinephrine was seen. This unusual stimulatory effect of epinephrine was blocked by propranolol indicating a beta-adrenergic mechanism for epinephrine. It is concluded that Ca-2+, which plays an essential role in the stimulus-secretion coupling, can alone initiate and cause sustained insulin release. 相似文献
8.
9.
Shoot and root growth of hydroponic maize (Zea mays L.) as influenced by K deficiency 总被引:1,自引:0,他引:1
Potassium (K) has major biophysical and biochemical functions in plant physiology. However, plant responses to K deficiency
at the whole plant level are not always clearly related to these well-known functions of K at the cellular level. The objective
of this study was to investigate the morphological response of maize to increasing K deficiency and test to what extent this
morphological response can be interpreted in the light of the simple model proposed by Leigh and Wyn Jones, suggesting that
biophysical functions are affected first. Maize was grown in a greenhouse under hydroponic conditions. For half of the plants,
K was removed from the nutrient solution from the 4th visible leaf stage. The K content in the starved plants dropped from
100 to 30 mM, and was not fully compensated by an increase in other cations. Leaf elongation rates were reduced on K-deprived
plants, whereas axile root elongation rates were slightly increased between 45°C days and 75°C days after starvation, and
reduced thereafter. During the first part of the starvation period, i.e. under moderate K deficiency (K concentration above
40 mM), all measured variables suggest that the whole plant response may be interpreted as the consequence of the reduced
leaf growth, probably due to insufficient turgor pressure or cell-wall extensibility. This general pattern of response is
in agreement with the model of Leigh and Wyn Jones. However, during the second part of the starvation period, i.e. under more
severe K deficiency (K concentration below 40 mM), malfunction of additional physiological processes (mostly related to biochemical
functions like photosynthetic processes) must be considered to explain the plant morphological response. 相似文献
10.
Activation of the action potential Na+ ionophore of cultured neuroblastoma cells by veratridine and batrachotoxin. 总被引:15,自引:0,他引:15
W A Catterall 《The Journal of biological chemistry》1975,250(11):4053-4059
The activation of the action potential Na+ ionophore by veratridine and batrachotoxin is time- and concentration-dependent and completely reversible. Batrachotoxin acts more slowly than veratridine. The concentration dependence of activation at equilibrium suggests reversible interaction of each toxin with a single class of independent sites having dissociation constants at physiologic ion concentrations of 80 plus or minus 13 muM for veratridine and 0.4 plus or minus muM for batrachotoxin. The maximum velocity of Na+ uptake at 50 mM Na+ is 128 plus or minus 12 nmol/min/mg in the presence of batrachotoxin compared to 48 plus or minus 4 nmol/min/mg in the presence of veratridine. Treatment of cells with excess veratridine in addition to batrachotoxin inhibits batrachotoxin-dependent 22-Na+ uptake. The concentration dependence of this inhibition suggests that it reflects competitive displacement of batrachotoxin from its binding site by veratridine. The activation by veratridine and batrachotoxin is inhibited in a competitive manner by divalent cations. The inhibition by divalent cations exhibits significant ion specificity with Mn-2+ greater than Co-2+ greater than Ni-2+ greater than Ca-2+ greater than Mg-2+ greater than Sr-2+. The inhibition constants (KI) for Ca-2+ are 0.84 mM for veratridine-dependent 22-Na+ uptake and 1.2 mM for batrachotoxin-dependent 22-Na+ uptake. The activation by veratridine and batrachotoxin is inhibited in a noncompetitive manner by tetrodotoxin. The apparent KD for tetrodotoxin as 11 plus or minus 1 nM in the presence of 150 mM Na+ and approximately 8.5 nM in 50 mM Na+. Divalent cations do not affect the apparent KD for tetrodotoxin. A hypothesis is presented which suggests that batrachotoxin, veratridine, and divalent cations interact with an activation site associated with the action potential Na+ ionophore, whereas tetrodotoxin interacts with a physically and functionally independent site involved in the transport of monovalent cations by the ionophore. 相似文献
11.
Akula Ramakrishna Parvatam Giridhar Gokare Aswathanarayana Ravishankar 《In vitro cellular & developmental biology. Plant》2011,47(6):667-673
High-frequency somatic embryogenesis was achieved in Coffea canephora using calcium ionophore A23187, which influences the influx of calcium into a cell. With 100 μM calcium ionophore and 5 mM
calcium, 85% and 70% of cultures produced embryogenic tissue, with 105 ± 7 and 95 ± 8 primary embryos from each callus mass
respectively. Medium supplemented with 100 μM EGTA (calcium chelator) or 1 mM verapamil (calcium channel blocker) significantly
reduced somatic embryogenesis. Calcium imaging studies were done to determine the relationship between morphogenetic response
and the cellular calcium levels. The calcium ionophore/calcium treatment was very effective in driving cellular machinery
toward embryogenesis. The embryos were regenerated into plantlets when cultured on MS medium supplemented with 5 mM calcium/100 μM
calcium ionophore A23187. Somatic embryogenesis-derived plants were successfully transferred to soil and grown to maturity
in the field. 相似文献
12.
The role of catalase in response of the yeast Saccharomyces cerevisiae to oxidative stress induced by hydrogen peroxide under starvation was investigated. It was shown that under conditions used in this study 0.5 mM H2O2 did not change the number of viable cells in the wild strain YPH250, but this parameter was decreased by 15% in the acatalsaemic strain YWT1. Cells treatment with 0.5 mM H2O2 for 30 min did not modify the levels of carbonyl proteins in the parental strain, but caused its 1.4-fold increase in the defective strain. The observed 1.5-fold activation of catalase in the wild strain cells in response to H2O2-stress suggests that under starvation conditions catalase can be involved in the yeast cell protection, particularly they can prevent oxidative modification of some antioxidant and associated enzymes. 相似文献
13.
Temporal relationships between maturational events and the onset of activation in response to divalent ionophore and to pricking were examined following in vitro exposure of Rana pipiens oocytes to desoxycorticosterone acetate (DOCA). Activation was evaluated on the basis of vitelline envelope elevation and cortical granule breakdown. Ionophore-induced activation was first observed after 18 hr of DOCA incubation, coincident with the time of separation of the vitelline envelope from the oocyte surface and 2–3 hr after breakdown of the germinal vesicle. Activation in response to pricking was not observed until 30 hr of DOCA incubation. Neither ionophore treatment nor pricking resulted in activation of oocytes that had not been incubated with DOCA. These results indicate that oocytes can be activated many hours earlier than previously demonstrated. The time of onset of the capacity for activation appears to be related to germinal vesicle breakdown and vitelline envelope separation. 相似文献
14.
Neurotensin regulation of TSH secretion in the rat 总被引:3,自引:0,他引:3
M C Sheppard R D Askew K I Shennan S Franks D B Ramsden 《Biochemical and biophysical research communications》1983,113(1):248-254
The ionophore A23187 (6.7 microM) increased the rates of formation of prostaglandins and cyclic AMP in suspensions of thioglycollate-elicited rat peritoneal macrophages. Both effects were inhibited by the calmodulin blocker trifluoperazine (50 microM) and the calcium channel blocker verapamil (500 microM). Inhibitors of phospholipase A2 and cyclo-oxygenase also blocked both actions of A23187. The stimulated prostaglandin formation was markedly reduced when the cells were preincubated with 8-bromo-cyclic AMP (1mM), dibutyryl cyclic AMP (1mM) or cholera toxin (500ng/ml). Addition of exogenous arachidonic acid (30 microM) alleviated this inhibition. We propose that the effect of A23187 on macrophages includes a 'self-limiting' mechanism whereby newly-synthesized prostaglandins can inhibit, via cyclic AMP, a step(s) prior to the transformation of arachidonic acid and thus modulate their own production. 相似文献
15.
Epidermal growth factor (EGF) induces a hyperpolarizing response of 5-20 mV amplitude in mouse mammary epithelial cells in culture. The amplitude of the hyperpolarizing response was reduced by more than 60% within several minutes after addition of blockers of voltage and/or Ca2+-dependent K+ channels such as tetraethylammonium (7 mM) or quinine (0.29 mM). Both nifedipine (0.15 mM), a blocker of the Ca2+ channel, and ruthenium red (2 mM), an inhibitor of the Ca2+-binding site, also reduced the amplitude of the hyperpolarizing response by more than 60%. The Ca2+ ionophore, A23187 (3.8 microM), induced a large hyperpolarization, which was 25-40 mV and lasted about 3 min. These data suggest that activity of the Ca2+-dependent K+ channel was involved in the EGF-induced hyperpolarizing response of the mammary epithelial cells. 相似文献
16.
Summary The effects of divalent cation ionophores, A23187 and X-537A, on the electrical membrane properties were investigated by using the soma membrane of the X-organ of the crayfish. They reduced the amplitude and maximum rate of rise of Ca-action potential in lower concentration. As the concentration increased, a reduction of membrane resistance and hyperpolarization occurred simultaneously. Further increase resulted in membrane depolarization with a further decrease in resistance. The threshold concentration of X537A was 100 times higher than that of A23187. These effects were reversible only when the application period was relatively short, while a longer application resulted in an incomplete reversibility or in no reversibility at all. The ionophore effect was facilitated in high Ca medium and diminished in low Ca medium. In Sr medium, the same effects on the resistance and the membrane potential were barely observable. TEA reduced the effects of A23187 but did not completely inhibit the effects. The Na-action potential was also reduced by the higher concentration of the ionophore. From these results it is concluded that the divalent cation ionophores, A23187 and X537A, carry divalent cation, Ca ions in a physiological medium, into the neuron soma through the membrane and the consequent increase of the intracellular divalent cations induces K conductance increase and that higher concentration of the ionophore induces the increase in the conductance of the other ion species, such as Na. 相似文献
17.
Frozen-thawed spermatozoa from Friesian bulls held at stud in Ireland were used to assess the effect of ionophore on motility, acrosome reaction and heterologous in vitro fertilization. Bovine spermatozoa penetrated zone-free hamster oocytes following treatment with calcium ionophore in the absence of bovine serum albumin (BSA) and in the presence of 10 mM caffeine. Sperm velocity was stimulated in concentrations of caffeine <2.5 mM following dilution with medium containing BSA. Sperm attachment to the plasmalemma showed no association with penetration rates of zona-free hamster oocytes. Penetrated oocytes in regimens with >0.1 mM ionophore did not progress through Meiosis II. Increasing concentrations of ionophore induced the acrosome reaction more rapidly, although this was associated with reduced motility. Hyperactive motility was observed in calcium ionophore-treated spermatozoa which were capable of penetrating zona-free hamster oocytes. Sperm velocity remained unchanged. whereas the track:vector ratio, a measurement of curvilinear movement, was reduced. This work may have important implications for the assessment of bovine fertility and cytogenetic analysis of bovine sperm. 相似文献
18.
Dispersed rat pancreatic acini were incubated in 0.5mM calcium medium with increasing concentrations of carbamylcholine, with or without the ionophore A23187 (10(-6)M). Addition of the ionophore reduced maximal amylase release, increased the maximal effective concentration of carbamylcholine and dramatically impaired the agonist's capacity to induce enzyme secretion at low concentration. The ionophore also abolished the inhibition of secretion observed at high carbamylcholine concentrations. These effects of the ionophore on the cholinergic secretory response cannot be explained by interaction at the muscarinic receptor since neither the Bmax, the affinity of the receptor for the [3H]QNB nor the binding of carbamylcholine were affected by the ionophore. It is suggested that for the conditions studied, the ionophore can interact with the secretory process at one or several points ulterior to the initial recognition site of carbamylcholine on its receptor. 相似文献
19.
The divalent ionophore A23187 can induce a non-propagated discharge of cortical granules in sea urchin eggs resulting in the elevation of partial fertilization membranes. This occurs when unfertilized eggs (1) partially overlap the margin of a solid film of the ionophore; (2) touch a Sephadex bead previously infiltrated with the ionophore; or (3) are exposed to solutions of ionophore for short periods (15–30 sec). These results demonstrate that the release of cortical granules does not automatically induce the discharge of neighboring granules. 相似文献
20.
M Matsuura Y Muneoka Y Shigenaka 《Comp. Biochem. Physiol. C, Comp. Pharmacol. Toxicol.》1984,79(2):357-361
The ABRM of M. edulis was immersed in KCl-EGTA solution (540 mM KCl + 5 mM EGTA) for 30 min. Then the muscle was returned to normal ASW, effects of the KCl-EGTA solution being examined on the ultrastructure of the neuromuscular junctions and also on the mechanical responses of the muscle to several kinds of stimuli. By the KCl-EGTA treatment of the muscle, synaptic vesicles in the nerve terminals at the junctions were found to be markedly deformed in shape and materially reduced in number. In most of the muscles tested, the contractile response to ACh and catch-relaxing responses to serotonin and dopamine were not depressed by the present treatment, though in some other muscles those responses were depressed a little. Contractile and catch-relaxing responses to repetitive electrical pulses of stimulation were markedly depressed or almost blocked by the treatment. All of the catch-relaxing responses to hexylamine (10(-3)M), phenylethylamine (10(-4)M), Na+-free ASW and 8-bromo-cyclic GMP (10(-3)M) were also markedly depressed or almost completely blocked. These results indicate that the function of intramuscular nerve fibres in the ABRM is markedly impaired by treating the muscle with KCl-EGTA solution while that of muscle fibres is little impaired. The treatment seems to be a useful method for denervation in the isolated ABRM. 相似文献