CHARACTERIZATION OF THE OFFSHORE MARINE MACROALGAE FROM THE NORTHWESTERN GULF OF MEXICO HARD BANK COMMUNITIES

The upper northwestern Gulf basin is characterized by a relatively shallow but well‐developed continental shelf with an extensive system of mid‐ and outer shelf hard banks of varying origin and composition. Observations done by our laboratory while participating in monitoring cruises to the National Marine Sanctuaries of the East and West Banks of the Texas Flower Gardens, Stetson Banks (TX) and Sonnier Banks (LA) reveal a surprisingly different floristic composition among the sites. Several range extensions, new macro‐algal records for the Gulf of Mexico, and new species imply a much more complex Algal Zone community than the one proposed by Rezak et al. (1985). Our ongoing project includes the development of a modern taxonomic, phylogenetic, morphological and genetic database essential to informed management for preservation of biological diversity. It also provides a framework of seasonal algal composition against which long‐term trends and anomalies in algal distribution and health of the Gulf of Mexico can be assessed at future dates.     

Applications of Satellite Ocean Color Sensors for Monitoring and Predicting Harmful Algal Blooms

The new satellite ocean color sensors offer a means of detecting and monitoring algal blooms in the ocean and coastal zone. Beginning with SeaWiFS (Sea Wide Field-of-view Sensor) in September 1997, these sensors provide coverage every 1 to 2 days with 1-km pixel view at nadir. Atmospheric correction algorithms designed for the coastal zone combined with regional chlorophyll algorithms can provide good and reproducible estimates of chlorophyll, providing the means of monitoring various algal blooms. Harmful algal blooms (HABs) caused by Karenia brevis in the Gulf of Mexico are particularly amenable to remote observation. The Gulf of Mexico has relatively clear water and K. brevis, in bloom conditions, tends to produce a major portion of the phytoplankton biomass. A monitoring program has begun in the Gulf of Mexico that integrates field data from state monitoring programs with satellite imagery, providing an improved capability for the monitoring of K. brevis blooms.     

ALGAL COMMUNITY STRUCTURE OF THE EAST AND WEST FLOWER GARDEN BANKS,NORTHWESTERN GULF OF MEXICO

The algal communities of the Flower Garden Banks National Marine Sanctuary have not been comprehensively evaluated and only a few dominant macroalgal species have been reported. This study utilizes both destructive and non‐destructive sampling techniques to characterize and taxonomically identify the ‘algal mat’ community structure. The East and West Flower Garden Banks are located on the outer continental shelf approximately 200 km off the Texas‐Louisiana coastline. The average depth of both banks is 100 m with the crest approximately 20 m from the surface. Harvest and photogrammetric samples were collected during two extended cruises to the Flower Garden Banks in October 1998 and March 1999. Forty, 0.25‐m² quadrats of standing stock material were randomly collected along with one hundred sixty‐one 0.25 m² photo‐quadrats from an average depth of 27 m. Photo‐transparencies were projected to an actual size grid with 25 random points. Four thousand twenty‐five transparency points were evaluated and used to calculate percent composition of algal cover. Harvest samples were used to characterize the “algal mat” composition, species richness, abundance, and biomass. Forty‐two species were identified from the samples representing 14 Orders. The “red algal mat” was the dominant algal coverage comprising 38.4% of all photogrammetric samples. This mat was primarily composed of members from the Order Ceramiales. Centroceras, Ceramium, and Polysiphonia comprised 33.4% of the mat, Anotrichium and Hypoglossum, 22.4%.     

THE PHYLOGENETIC RELATIONSHIPS OF THE PHAEOTHAMNIOPHYCEAE, WITH COMMENTS ON CERTAIN CAPSOID MEMBERS OF THE CHRYSOPHYCEAE

The algal communities of the Flower Garden Banks National Marine Sanctuary have not been comprehensively evaluated and only a few dominant macroalgal species have been reported. This study utilizes both destructive and non-destructive sampling techniques to characterize and taxonomically identify the 'algal mat' community structure. The East and West Flower Garden Banks are located on the outer continental shelf approximately 200 km off the Texas-Louisiana coastline. The average depth of both banks is 100 m with the crest approximately 20 m from the surface. Harvest and photogrammetric samples were collected during two extended cruises to the Flower Garden Banks in October 1998 and March 1999. Forty, 0.25-m² quadrats of standing stock material were randomly collected along with one hundred sixty-one 0.25 m² photo-quadrats from an average depth of 27 m. Photo-transparencies were projected to an actual size grid with 25 random points. Four thousand twenty-five transparency points were evaluated and used to calculate percent composition of algal cover. Harvest samples were used to characterize the "algal mat" composition, species richness, abundance, and biomass. Forty-two species were identified from the samples representing 14 Orders. The "red algal mat" was the dominant algal coverage comprising 38.4% of all photogrammetric samples. This mat was primarily composed of members from the Order Ceramiales. Centroceras, Ceramium , and Polysiphonia comprised 33.4% of the mat, Anotrichium and Hypoglossum , 22.4%.     

Assessment of littoral algal diversity from the northern Gulf of Mexico using environmental DNA metabarcoding

Traditional methods for algal biodiversity monitoring are costly and time inefficient because they rely on high‐level taxonomic expertise to address species identity problems involving phenotypic plasticity and morphological convergence. These problems are exacerbated in regions such as the Gulf of Mexico, that has a limited history of phycological exploration, but that are economically important or threatened by numerous anthropogenic stressors. Given the high pace of disturbance to natural systems, there is a critical need for expedient and cost‐effective tools for the study of benthic algal communities. Here we document the use of environmental DNA metabarcoding, using the partial LSU rDNA and 23S rDNA plastid molecular markers, to elucidate littoral algal diversity in the Northern Gulf of Mexico. We assigned 73.7% of algal OTUs to genus and 59.6% to species ranks. Our current study detected molecular signals for 35 algal/protist species with no previous reports in the Gulf of Mexico, thus providing an important, molecular‐validated, baseline of species richness for this region. We also make several bioinformatic recommendations for the efficient use of high‐throughput sequence data to assess biological communities.     

Persistence of coral assemblages at East and West Flower Garden Banks,Gulf of Mexico

Since 1989 a federally supported long-term coral reef monitoring program has focused on two study sites atop East and West Flower Garden Banks in the northwestern Gulf of Mexico. We examined 25 yr of benthic cover data to provide a multi-decadal baseline and trend analysis of the community structure for this coral reef system. Despite global coral reef decline in recent decades, mean coral cover at East and West Flower Garden Banks was above 50% for the combined 25 yr of continuous monitoring, and represented a stable coral community. However, mean macroalgal cover increased significantly between 1998 and 1999, rising from approximately 3 to 20%, and reaching a maximum above 30% in 2012. In contrast to many other shallow water reefs in the Caribbean region, increases in mean macroalgal cover have not been concomitant with coral cover decline at the Flower Garden Banks.     

Diel reproduction and vertical distributions of the marine cladocerans, Evadne tergestina and Penilia avirostris, in contrasting coastal environments

Evadne tergestina and Penilia avirostris were sampled duringseveral diel cycles in the well-mixed, turbid Inland Sea ofJapan and the more stratified, less turbid Gulf of Mexico shelf.Parthenogenetic female Evadne contained embryos with pigmentedeyes only at night, and apparently released neonates near dawnor in the morning. There was no significant diel vertical migrationin the Gulf of Mexico, and a ‘reverse’ migrationin the Inland Sea. Female Penilia could contain mature embryosat any time, but were most likely to do so at night. Peniliadid not migrate dielly, and in the Gulf of Mexico was concentratedin a nepheloid layer near the bottom.     

Sedimentological history of Bryant Canyon area, northwest Gulf of Mexico, during the last 135 kyr (Marine Isotope Stages 1-6): A proxy record of Mississippi River discharge

Bryant and Eastern Canyons are located in northwest Gulf of Mexico, and are characterized by a complex sedimentological history related to glacioeustatic cycles, river discharges, and interactions between depositional and halokinetic processes. This study is based on detailed sedimentological analysis from forty-eight long cores from these two canyons. This paper determines the evolutionary history of the canyons and assesses the response of sedimentary processes to morphological, climatic, hydrological, and sea-level changes.During the last glaciation, the upper and middle continental slope was supplied with sediments by low density turbidity currents derived from the depositional segregation (deposition of the coarsest material in the most proximal locations) of large turbidity currents initiated on the outer shelf. The lower continental slope was supplied with sediment by westward flowing bottom currents, originated from the entrainment of the most diluted wash-load and tails of turbidity currents from the Mississippi Fan.Bryant and Eastern Canyon systems were active during the penultimate glaciation, Marine Isotope Stage (MIS) 6, and were supplied with sediments by an ancestral shelf-margin Mississippi River delta. Gravity flows transported enormous amounts of sediment to the continental slope and abyssal plain of the northwest Gulf of Mexico. The sea-level rise at MIS 5 led to confinement of river-sourced sediments to the widespread continental shelf of the northwest Gulf of Mexico, and consequently to the cessation of gravity flows. During the first 40 kyr of MIS 5, salt diapirs transformed the canyons into a network of intraslope basins.The sea level dropped to the mid-shelf during MIS 3 and 4, but never reached the shelf-break, and therefore, river-sourced sediments remained largely confined to the shelf. However, seaward sediment transportation was achieved occasionally through turbidity currents related to sediment failures, storms, and high-river discharges. Four high river discharge events have been identified during this period. The first three were centred at 37, 45, and 53 cal ka BP. The last high river discharge occurred at the end of MIS 3 (29.4-33.2 cal ka BP), and resulted in the deposition of closely-spaced, mud turbidites over the entire continental slope. The Laurentide Ice Sheet (LIS) was restricted north of the upper Mississippi River valley during 60 to ∼ 30 cal ka BP and therefore, the high river discharge events are interpreted as melt-water events, related to brief southward advancements of the LIS, which resulted in the flooding of Mississippi River. The extensive lowering of sea level during the last glacial maximum (MIS 2) resulted in the almost direct discharge of Mississippi River sediments to the upper continental slope leading to the development of abundant turbidity currents. Eleven wet-dry cycles during this period are defined; they probably originated from episodic subglacial melt-water floods, released from southern parts of the LIS.The last deglaciation event is characterized by the development of a major melt water event at 16.5-13 cal ka BP that resulted in the deposition of distinct, organic-rich sediments. At about 13 cal ka BP, the melt water discharges of the LIS in North America switched from the Mississippi River to either the St. Lawrence or Mackenzie River valleys, causing the domination of hemipelagic sedimentation on the continental slope of the northwest Gulf of Mexico. Isotopic data indicate that melt-water discharges returned to the Mississippi River Valley at ∼ 11.4 cal ka BP. The absence of any sedimentological indication on the continental slope of the northwest Gulf of Mexico of the return of the melt-water discharges to the Mississippi River is attributed to the confinement of river-sourced sediments on the continental shelf due to the rise of the sea level.     

Colony morphology,age structure,and relative growth of two gorgonian corals,Leptogorgia hebes (Verrill) and Leptogorgia virgulata (Lamarck), from the northerm Gulf of Mexico

Along the northeastern Gulf of Mexico coast, Leptogorgia hebes is the predominant gorgonian at two inner shelf (22 and 27 m) sites, while Leptogorgia virgulata dominates a third shallow (1–2m), inshore site. The axial skeleton of both species is composed of concentric rings which appeared to exhibit annual periodicity. Although age structure of the populations differed between shelf sites, neither colony growth rates, estimated from direct measurements of ring widths plotted on Walford graphs, nor overall branching complexity, determined from bifurcation ratios (R_b), differed between the shelf populations. Larger individuals at the 27m shelf site were simply older than those at the shallower (22m) shelf site. Walford plots of growth increments revealed colony growth of the shallow inshore species to be similar to that of the offshore species.     

Genetic connectivity in scleractinian corals across the Northern Gulf of Mexico: oil/gas platforms, and relationship to the Flower Garden Banks

The 3,000 oil/gas structures currently deployed in the northern Gulf of Mexico (GOM) provide hard substratum for marine organisms in a region where such has been rare since the Holocene. The major exception to this are the Flower Garden Banks (FGB). Corals are known to have colonized oil/gas platforms around the FGB, facilitating biogeographic expansion. We ask the question, what are the patterns of genetic affinity in these coral populations. We sampled coral tissue from populations of two species occurring on oil and gas platforms: Madracis decactis (hermatype) and Tubastraea coccinea (invasive ahermatype). We sampled 28 platforms along four transects from 20 km offshore to the continental shelf edge off 1) Matagorda Island, TX; 2) Lake Sabine, TX; 3) Terrebonne Bay, LA; and 4) Mobile, AL. The entire population of M. decactis was sampled between depths of 5 m and 37 m. T. coccinea populations were sub-sampled. Genetic variation was assessed using the PCR-based Amplified Fragment Length Polymorphisms (AFLPs). Data were analyzed via AFLPOP and STRUCTURE. Genetic connectivity among M. decactis platform populations was highest near the FGB and decreased to the east. Connectivity increased again in the eastern sector, indicating isolation between the populations from different sides of the Mississippi River (Transects 3 and 4). A point-drop in genetic affinity (relatedness) at the shelf edge south of Terrebonne Bay, LA indicated a population differing from all others in the northern GOM. Genetic affinities among T. coccinea were highest in the west and decreased to the east. Very low genetic affinities off Mobile, AL indicated a dramatic difference between those populations and those west of the Mississippi River, apparently a formidable barrier to larval dispersal.     

Genetic differences within and between species of deep-sea crabs (chaceon) from the North Atlantic Ocean

The deep-sea red crab Chaceon quinquedens is a commercially important crustacean on the Atlantic continental shelf and slope of North America. To assess genetic subdivision in C. quinquedens, we examined the nucleotide sequence of the mitochondrial 16S rDNA gene and the internal transcribed spacers (ITS) of the nuclear ribosomal repeat in samples from southern New England and the Gulf of Mexico. We compared those data to sequences from two congeners, a sympatric species from the Florida coast, C. fenneri, and an allopatric eastern Atlantic species, C. affinis. The 16S rDNA data consisted of 379 aligned nucleotides obtained from 37 individuals. The greatest genetic difference among geographical groups or nominal species was between C. quinquedens from southern New England and C. quinquedens from the Gulf of Mexico. Haplotypes from these two groups had a minimum of 10 differences. All 11 C. fenneri samples matched the most common haplotype found in C. quinquedens from the Gulf of Mexico, and this haplotype was not detected in C. quinquedens from southern New England. The three haplotypes of C. affinis were unique to that recognized species, but those haplotypes differed only slightly from those of C. fenneri and C. quinquedens from the Gulf of Mexico. Based on 16S rDNA and ITS data, genetic differences between C. quinquedens from southern New England and the Gulf of Mexico are large enough to conclude that these are different fishery stocks. Our results also indicate that the designation of morphological species within the commercially important genus Chaceon is not congruent with evolutionary history. The genetic similarity of C. affinis from the eastern Atlantic and C. quinquedens from the Gulf of Mexico suggests these trans-Atlantic taxa share a more recent common history than the two populations of "C. quinquedens" that we examined.     

Defining and Dividing the Greater Caribbean: Insights from the Biogeography of Shorefishes

The Greater Caribbean biogeographic region is the high-diversity heart of the Tropical West Atlantic, one of four global centers of tropical marine biodiversity. The traditional view of the Greater Caribbean is that it is limited to the Caribbean, West Indies, southwest Gulf of Mexico and tip of Florida, and that, due to its faunal homogeneity, lacks major provincial subdivisions. In this scenario the northern 2/3 of the Gulf of Mexico and southeastern USA represent a separate temperate, “Carolinian” biogeographic region. We completed a comprehensive re-assessment of the biogeography of the Greater Caribbean by comparing the distributions of 1,559 shorefish species within 45 sections of shelf waters of the Greater Caribbean and adjacent areas. This analysis shows that that the Greater Caribbean occupies a much larger area than usually thought, extending south to at least Guyana, and north to encompass the entire Carolinian area. Rather than being homogenous, the Greater Caribbean is divided into three major provinces, each with a distinctive, primarily tropical fauna: (1) a central, tropical province comprising the West Indies, Bermuda and Central America; (2) a southern, upwelling-affected province spanning the entire continental shelf of northern South America; and (iii) a northern, subtropical province that includes all of the Gulf of Mexico, Florida and southeastern USA. This three-province pattern holds for both reef- and soft bottom fishes, indicating a general response by demersal fishes to major variation in provincial shelf environments. Such environmental differences include latitudinal variation in sea temperature, availability of major habitats (coral reefs, soft bottom shorelines, and mangroves), and nutrient additions from upwelling areas and large rivers. The three-province arrangement of the Greater Caribbean broadly resembles and has a similar environmental basis to the provincial arrangement of its sister biogeographic region, the Tropical Eastern Pacific.     

Maastrichtian cephalopods from Cerralvo, north-eastern Mexico

Sediments of the Méndez Formation near Cerralvo, north-eastern Mexico, yield an abundant and diverse Maastrichtian ammonite assemblage. A total of 23 species referred to 18 genera are described, in addition to the possible coleoid Naefia neogaeia . The assemblage is considered to be of early Maastrichtian age on the basis of ammonite occurrences and has been dated to the lower Maastrichtian biozone CF 7 by planktic foraminifera. None of the ammonite species has been reported previously from the Méndez Formation and most species are recorded from Mexico for the first time. In addition to faunal elements known from other Gulf of Mexico localities [ Baculites ovatus , Nostoceras ( N. ) alternatum , N. ( N. ) colubriformis , N. ( N. ) rugosum , Solenoceras reesidei ] the assemblage is characterized by cosmopolitan (e.g. Anagaudryceras politissimum , Desmophyllites diphylloides , Diplomoceras cylindraceum , Gaudryceras kayei , Phyllopachyceras forbesianum , and Pseudophyllites indra ) and Tethyan elements [e.g. Brahmaites ( Anabrahmaites ) vishnu , Fresvillia constricta , Hauericeras rembda , Solenoceras texanum, Tetragonites superstes ]. Some ammonite species have been known from the Indopacific region a [e.g. Fresvillia aff. F. teres , Neophylloceras ( Hypophylloceras ) hetonaiense , Zelandites varuna ] and are clearly cold-water species. The composition of the assemblage contrasts with other Gulf of Mexico faunas which is related to bathymetry.     

Hierarchical population structure and habitat differences in a highly mobile marine species: the Atlantic spotted dolphin

Recent molecular studies have shown that highly mobile species with continuous distributions can exhibit fine‐scale population structure. In this context, we assessed genetic structure within a marine species with high dispersal potential, the Atlantic spotted dolphin (Stenella frontalis). Using 19 microsatellite loci and mitochondrial control region sequences, population structure was investigated in the western North Atlantic, the Gulf of Mexico and the Azores Islands. Analyses of the microsatellite data identified four distinct genetic clusters, which were supported by the control region sequences. The highest level of divergence was seen between two clusters corresponding to previously described morphotypes that inhabit oceanic and shelf waters. The combined morphological and genetic evidence suggests these two lineages are on distinct evolutionary trajectories and could be considered distinct subspecies despite their parapatry. Further analysis of the continental shelf cluster resulted in three groups: animals inhabiting shelf waters in the western North Atlantic, the eastern Gulf of Mexico and the western Gulf of Mexico. Analyses of environmental data indicate the four genetic clusters inhabit distinct habitats in terms of depth and sea surface temperature. Contemporary dispersal rate estimates suggest all of these populations should be considered as distinct management units. Conversely, no significant genetic differentiation was observed between S. frontalis from offshore waters of the western North Atlantic and the Azores, which are separated by approximately 4500 km. Overall, the hierarchical structure observed within the Atlantic spotted dolphin shows that the biogeography of the species is complex because it is not shaped solely by geographic distance.     

Seasonal and spatial variation of phytoplankton assemblages, biomass and cell size from spring to summer across the north-eastern New Zealand continental shelf

The composition, biomass and cell size of phytoplankton taxonomicgroups were determined in the Hauraki Gulf and adjacent shelfof north-eastern New Zealand. In early spring, on the innershelf, over-winter mixing and upwelling supported a bloom dominatedby large, chain-forming diatoms in a moderately turbulent watercolumn. The bloom declined in late spring because of nutrientlimitation, and the assemblage evolved initially toward smalldiatoms, and eventually to co-occurrence of dinoflagellates,small nanoflagellates and picophytoplankton in early and latesummer. Mid- to outer-shelf biomass was much lower than inshore,and was dominated by small or motile taxa which were probablylimited by grazing and light. In early summer, strong upwellingdisplaced inner shelf phytoplankton to beyond the shelf edge,whilst enriching the shelf with nutrients. However, shelf phytoplanktonbiomass increased only after the relaxation of upwelling. TheHauraki Gulf was strongly stratified from early spring throughlate summer. The flora were seasonally less variable than onthe shelf, with a thecate dinoflagellate-dominated flora inearly spring, replaced post-bloom by the co-occurrence of presumablylow-nutrient-adapted autotrophic and/or heterotrophic dinoflagellates(most of which were non-thecate, and some toxic), nanoflagellatesand picophytoplankton. The succession in floristics was consistentwith a change from an autotrophic toward a heterotrophic ecosystemfrom spring to summer. Implications for secondary production,and vertical and lateral organic carbon export on the shelf,are discussed.     

A new sponge-inhabiting amphipod species (Crustacea, Gammaridea, Sebidae) from the Veracruz Coral Reef System, southwestern Gulf of Mexico

A new species of the genus Seba Bate (Amphipoda: Sebidae) is described from the Veracruz Coral Reef System, Veracruz, south-western Gulf of Mexico. The specimens were found in association with a sponge, Ircinia fistularis, at depths from 8 to 10 m. Seba alvarezi n. sp. can be distinguished from closely related species mainly by the presence of a short, two-articulated accessory flagellum, three apical setae on article 3 of the mandible palp, and by the absence of apical setae on the inner lobe of maxilla 1. The new species is compared to closely related species in the family, and an identification key to the species of Seba known from the Gulf of Mexico and the Caribbean Sea is given.     

Numerical increases and distributional shifts of Chrysaora quinquecirrha (Desor) and Aurelia aurita (Linné) (Cnidaria: Scyphozoa) in the northern Gulf of Mexico

Fisheries resource trawl survey data from the National Marine Fisheries Service from a 11–13-year period to 1997 were examined to quantify numerical and distributional changes of two species of northern Gulf of Mexico scyphomedusae: the Atlantic sea nettle, Chrysaora quinquecirrha (Desor), and the moon jelly, Aurelia aurita (Linné). Trawl surveys were grouped into 10 statistical regions from Mobile Bay, Alabama to the southern extent of Texas, and extended seaward to the shelf break. Records of summertime C. quinquecirrha medusa populations show both an overall numerical increase and a distributional expansion away from shore in the down-stream productivity field of two major river system outflows: Mobile Bay and the Mississippi-Atchafalaya Rivers. In addition, there is a significant overlap between summer C. quinquecirrha and lower water column hypoxia on the Louisiana shelf. In trawl surveys from the fall, A. aurita medusae showed significant trends of numerical increase in over half of the regions analyzed. For both species, there were statistical regions of no significant change, but there were no regions that showed significant decrease in number or distribution. The relationships between natural and human-induced (e.g. coastal eutrophication, fishing activity and hard substrate supplementation) ecosystem modifications are very complex in the Gulf of Mexico, and the potential impact of increased jellyfish populations in one of North America's most valuable fishing grounds is a most critical issue. Several hypotheses are developed and discussed to guide future research efforts in the Gulf of Mexico.     

An Analysis of Artificial Reef Fish Community Structure along the Northwestern Gulf of Mexico Shelf: Potential Impacts of “Rigs-to-Reefs” Programs

Artificial structures are the dominant complex marine habitat type along the northwestern Gulf of Mexico (GOM) shelf. These habitats can consist of a variety of materials, but in this region are primarily comprised of active and reefed oil and gas platforms. Despite being established for several decades, the fish communities inhabiting these structures remain poorly investigated. Between 2012 and 2013 we assessed fish communities at 15 sites using remotely operated vehicles (ROVs). Fish assemblages were quantified from standing platforms and an array of artificial reef types (Liberty Ships and partially removed or toppled platforms) distributed over the Texas continental shelf. The depth gradient covered by the surveys (30–84 m) and variability in structure density and relief also permitted analyses of the effects of these characteristics on fish richness, diversity, and assemblage composition. ROVs captured a variety of species inhabiting these reefs from large transient piscivores to small herbivorous reef fishes. While structure type and relief were shown to influence species richness and community structure, major trends in species composition were largely explained by the bottom depth where these structures occurred. We observed a shift in fish communities and relatively high diversity at approximately 60 m bottom depth, confirming trends observed in previous studies of standing platforms. This depth was also correlated with some of the largest Red Snapper captured on supplementary vertical longline surveys. Our work indicates that managers of artificial reefing programs (e.g., Rigs-to-Reefs) in the GOM should carefully consider the ambient environmental conditions when designing reef sites. For the Texas continental shelf, reefing materials at a 50–60 m bottom depth can serve a dual purpose of enhancing diving experiences and providing the best potential habitat for relatively large Red Snapper.     

ALGICIDAL BACTERIA ACTIVE AGAINST GYMNODINIUM BREVE (DINOPHYCEAE). I. BACTERIAL ISOLATION AND CHARACTERIZATION OF KILLING ACTIVITY1,3

Interactions between bacteria and species of harmful and/or toxic algae are potentially important factors affecting both the population dynamics and the toxicity of these algae. Recent reports of bacteria lethal to certain harmful algal bloom (HAB) species, coupled with a rapidly evolving interest in attempting to minimize the adverse effects of HABs through various prevention, control, and mitigation strategies, have focused attention on defining the role of algicidal bacteria in bloom termination. The aim of the present study was to determine whether algicidal bacteria active against Gymnodinium breve Davis, a dinoflagellate responsible for frequent and protracted red tides in the Gulf of Mexico, are present in the waters of the west Florida shelf. To date, we have isolated two bacterial strains from this region lethal to G. breve and have begun to characterize the algicidal activity of one of these strains, 41-DBG2. This bacterium, a yellow-pigmented, gram-negative rod, was isolated from waters containing no detectable G. breve cells, suggesting that such bacteria are part of the ambient microbial community and are not restricted to areas of high G. breve abundance. Strain 41-DBG2 produced a dissolved algicidal compound(s) that was released into the growth medium, and the algicide was effective against the four Gulf of Mexico G. breve isolates tested as well as a closely related HAB species that also occurs in this region, Gymnodinium mikimotoi Miyake et Kominami ex Oda. Nonetheless, data showing that a nontoxic isolate of Gymnodinium sanguineum Hirasaka from Florida Bay was not affected indicate that the algicidal activity of this bacterium does exhibit a degree of taxonomic specificity. Our efforts are currently being directed at resolving several critical issues, including the identity of the algicide(s), the mechanisms regulating its production and ability to discriminate between target algal species, and how the growth rate of 41-DBG2 is affected by the presence of G. breve cells. We have also proposed a conceptual model for interactions between algicidal bacteria and their target species to serve as a testable framework for ensuing field studies.     

Richness and endemism of helminth parasites of freshwater fishes in Mexico

Distribution records of 152 adult helminth taxa parasites of freshwater fishes in Mexico were analysed to determine areas of high richness and endemism. Distribution maps were prepared for each taxon and overlaid onto a map of Mexico divided into 1 × 1 degree grid-cells. Richness was determined by counting recorded helminth species in each grid-cell. A corrected weighted endemism index was calculated for each grid-cell, and the relationship between richness and endemicity was analysed with an Olmstead–Tukey corner test of association. Five areas of high richness and endemism were identified: (1) Los Tuxtlas and the Papaloapan river basin, on the Gulf of Mexico; (2) the Grijalva-Usumacinta basin near the Gulf of Mexico coastal plain; (3) the Yucatan Peninsula; (4) the Sierra de Manantlán Biosphere Reserve in western Mexico; and (5) the Pátzcuaro lake, in central Mexico. The distribution of richness and endemism of helminth parasites of freshwater fishes in Mexico is congruent with distributional patterns described for other freshwater taxa in Mexico. Patterns of richness and/or endemism in the studied areas can be explained by the ichthyological composition of their bodies of water. The present study establishes an objective way of analysing the relationship between richness and endemicity, and suggests that helminths can make valuable contributions to regionalization of geographical areas and for identification of rich and biologically complex areas with potential for conservation of aquatic systems.  © 2008 The Linnean Society of London, Biological Journal of the Linnean Society , 2008, 94 , 435–444.