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虾青素是一种天然类胡萝卜素,具有较强的抗氧化作用。法夫红酵母是一种能合成虾青素的真菌。本文主要概述法夫红酵母高产虾青素菌株的育种、高产株选择、培养条件和虾青素的提取检测方法。 相似文献
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法夫红酵母高产虾青素菌株的研究概况 总被引:8,自引:0,他引:8
虾青素是一种天然类胡萝卜素,具有较强的抗氧化作用。法夫红酵母是一种能合成虾青素的真菌。本文主要概述法夫红酵母高产虾青素菌株的育种、高产株选择、培养条件和虾青素的提取检测方法 相似文献
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法夫酵母生物合成虾青素的研究 总被引:4,自引:0,他引:4
本文对法夫酵母是青素的生命合成进行了研究。实验结果表明,法夫酵母在YEPD培养基中生长时表现出二次生出现象,细胞内虾青素的合成是与细胞季开偶联的,在约66h达一在积累,3.4μg/ml。 相似文献
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法夫酵母高产虾青素的选育及发酵条件的优化 总被引:2,自引:0,他引:2
对法夫酵母原始菌株进行了紫外线-氯化锂复合诱变,获得了一株遗传性状稳定、在25℃条件下虾青素高产的菌株UL-40。其类胡萝卜素产量为7.10mg/L,经HPLC测定,其虾青素产量为643.97μg/g,比出发菌株提高了249.87%。利用SAS软件中的Plackett-Burman设计对发酵温度、初始pH值以及发酵培养基的八种组分进行优化组合,从中选出发酵温度、初始pH值和Corn steep liquor浓度为重要因素,通过响应面分析法建立了模型并从该模型中计算出在发酵温度为16.78℃、初始pH为4.73和CSL浓度为7.06mg/L时预测的最大响应值为3.9407mg/L,经实验证实此点的实测值为3.9261mg/L,证明模型是有效的并且存在极大值点。采用优化后的发酵条件及发酵培养基,法夫酵母生产虾青素的产量较原始发酵培养基提高了20.4%。 相似文献
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法夫酵母(Phaffiarhodozyma)PLX朅ll菌株能够发酵纤维素酶水解物进行虾青素的生物合成。纤维素的酶解物主要为纤维二糖和葡萄糖,在另外添加适量其它营养物后可被法夫酵母发酵用于生长及合成虾青素。摇瓶试验结果表明,培养108h,法夫酵母的生物量可达2.3g/L,虾青素的产率达913.4g/g干细胞,虾青素体积产率为2.1mg/L。在2L罐的发酵试验中,法夫酵母的生物量可达3.23g/L(第96h),虾青素的产率达581.4g/g干细胞,虾青素体积产率达1.88mg/L。 相似文献
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法夫酵母PLX-All发酵纤维素酶水解物合成虾青素 总被引:2,自引:0,他引:2
法夫酵母(Phaffia rhodozyma)PLX-All菌株能够发酵纤维素酶水解物进行虾青素的生物合成。纤维素的酶解物主要为纤维二糖和葡萄糖,在另外添加适量其它营养物后可被法夫酵母发酵用于生长及合成虾青素。摇瓶试验结果表明,培养108h,法夫酵母的生物量可达2.3g/L,虾青素的产率达913.4g/g干细胞,虾青素体积产率为2.1mg/L。在2L罐的发酵试验中,法夫酵母的生物量可达3.23g/L(第96h),虾青素的产率达581.4g/g干细胞,虾青素体积产率达1.88mg/L。 相似文献
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法夫酵母PLX—A11发酵纤维素酶水解物合成虾青素 总被引:1,自引:1,他引:1
法夫酵母(Phaffia rhodozyma)PLX-A11菌株能够发酵纤维素酶水解物进行虾青素的生物合成。纤维素的酶解物主要为纤维二糖和葡萄糖,在另外添加适量其它营养物后可被法夫酵母发酵用于生长及俣成虾青素。摇瓶试验结果表明,培养108h,法夫酵母的生物量可达2.3g/L,虾青素的产率达913.4μg/g干细胞,虾青素体积产率为2.1mg/L。在2L罐的发酵试验中,法夫酵母的生物量可达3.23g 相似文献
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法夫酵母生产虾青素发酵条件的研究 总被引:2,自引:0,他引:2
方法:分别进行了接种时间、摇床转速、接种量和装液量对法夫酵母细胞生产虾青素摇瓶发酵过程影响的实验,比较了DMSO法、酸热法、碱法和自溶法等破壁方法和提取溶剂之间的差别,测定了法夫酵母生长过程中的生物量、类胡萝卜素产量和培养基中的残糖。结果:确定了最佳的摇瓶发酵条件为:种瓶至发酵摇瓶的接种时间为40h,摇床转速为160r/min,接种量为10%,装液量为50mL;DMSO法和丙酮分别为合适的破壁方法和提取溶剂。结论:初步确定发酵的基本条件,为进行法夫酵母高产虾青素菌种的筛选以及发酵培养基的优化奠定了基础。 相似文献
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Increased astaxanthin production by a Phaffia rhodozyma mutant grown on date juice from Yucca fillifera 总被引:1,自引:0,他引:1
J Ramírez M L Nuñez R Valdivia 《Journal of industrial microbiology & biotechnology》2000,24(3):187-190
The wild strain and the astaxanthin-overproducing mutant strain 25–2 of Phaffia rhodozyma were analyzed in order to assess their ability to grow and synthesize astaxanthin in a minimal medium composed of g L−1: KH2PO4 2.0; MgSO4 0.5; CaCl2 0.1; urea 1.0 and supplemented with date juice of Yucca fillifera as a carbon source (yuca medium). The highest astaxanthin production (6170 μg L−1) was obtained at 22.5 g L−1 of reducing sugars. The addition of yeast extract to the yuca medium at concentrations of 0.5–3.0 g L−1 inhibited astaxanthin synthesis. The yuca medium supported a higher production of astaxanthin, 2.5-fold more than that observed
in the YM medium. Journal of Industrial Microbiology & Biotechnology (2000) 24, 187–190.
Received 14 July 1999/ Accepted in revised form 02 December 1999 相似文献
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Víctor Cifuentes Germán Hermosilla Claudio Martínez Ruben León Guido Pincheira Antonio Jiménez 《Antonie van Leeuwenhoek》1997,72(2):111-117
In this work we establish the chromosomal composition of a wild-type, one astaxanthin and two -carotene overproducer strains of the red yeast Phaffia rhodozyma. The method used has been pulsed field gel electrophoresis, which has determined 9 DNA chromosomal bands in the yeast genome. The two largest bands are triplets and two other bands, VI and VIII, seem to be doublets. The size of the chromosomal bands varies between 0.35 and 2.5 Mb, suggesting a genome size of 25 Mb. The technique used, complemented with hybridization assays using specific DNA probes, provides direct information about the genomic organization of P. rhodozyma. We have also cloned and located in chromosomal bands different DNA sequences that code for the translation elongation factor 1 alpha (ef-1), a 7.6 kb BamHI fragment of repetitive DNA (possibly rDNA) and a randomly chosen fragment (named locus R2). Additionally, we have detected a chromosomal length polymorphism between wild-type strains and mutant strains affecting carotenogenesis obtained in our laboratory. 相似文献
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Addition of ethanol (0.2%) to cultures of the yeast Phaffia rhodozyma increased the specific rate of carotenoid production [(carotenoid)(cell mass)−1(time)−1]. The incremental increase in carotenoid synthesis with ethanol was highest in carotenoid-hyperproducing strains. Ethanol
increased carotenoid production when it was added at various points during the lag and active growth phases. Ethanol increased
alcohol dehydrogenase and hydroxy-methyl-glutaryl-CoA (HMG-CoA) reductase activities. Our results indicate that increased
carotenoid production by ethanol is associated with induction of HMG-CoA reductase and possibly activation of oxidative metabolism.
Received 24 December 1996/ Accepted in revised form 27 May 1997 相似文献
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为了评价虾青素高产菌株-法夫酵母JMU-MVP14的生产性能及建立虾青素高产发酵技术,通过测定糖、生物量、虾青素产量、总类胡萝卜素产量等发酵参数,用摇瓶试验对比了法夫酵母JMU-MVP14和出发菌株的差异,用7 L罐试验对比了pH值调控方式及补料培养基成分对发酵的影响,用1 m3罐试验评估了法夫酵母JMU-MVP14高密度发酵虾青素的产量水平。摇瓶发酵结果表明,法夫酵母JMU-MVP14虾青素及总类胡萝卜素的细胞产率分别达到6.01 mg/g及10.38 mg/g;7 L罐分批发酵试验结果表明,自动流加调 相似文献
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【目的】研究乳酸钠(一种糖代谢产物)的加入对法夫酵母JMU-VDL668发酵过程中细胞生长和虾青素合成的影响。【方法】分别在摇瓶和7 L发酵罐实验基础上,采用代谢通量分析的方法分析添加乳酸钠对法夫酵母菌株JMU-VDL668合成虾青素代谢流的影响。【结果】在7 L发酵罐实验中添加乳酸钠,虾青素产量最高可达17.70 mg/L,与对照组相比提高26%。代谢通量分析表明,乳酸钠可以调节丙酮酸、乙酰辅酶A节点处的代谢通量分布,乳酸在乳酸脱氢酶的作用下可以直接进入代谢网络的后半程,乙酰辅酶A的通量和进入TCA循环的通量得到了显著加强。【结论】乳酸钠的加入提供了更多的乙酰辅酶A等前体物质和能量供给,因此促进了虾青素的合成。 相似文献
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To optimize biomass and carotenoid production by Phaffia rhodozyma in pH-stat cultures, two methods of feeding glucose were studied. In the first method, which is comparatively simple to operate, the glucose feeding set point (pH 5.02) was higher than the culture pH (5.00) and P. rhodozyma grew at a low specific growth rate (=0.055 h–1). In the second method, the glucose feeding set point (pH 4.98) was lower than the culture pH (5.00) and the yeast grew at a specific growth rate of =0.095 h–1. With the second method of glucose feeding, which is more complex and in order to prevent overfeeding of glucose, a time interval was added to the control strategy of the glucose pump and allowed to expire before the next dose of glucose was added. The length of the time interval affected biomass and carotenoid production. A critical time interval (Tc) was defined. In pH-stat cultures of P. rhodozyma, it was found that if the time interval was set longer than the critical time interval, the yeast did not grow. 相似文献
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R D Medwid 《Journal of industrial microbiology & biotechnology》1998,21(4-5):228-232
The ploidy of the red yeast Phaffia rhodozyma was evaluated using flow cytometric analyses of propidium iodide- stained cells and mutagenic inactivation kinetics. Our
findings suggest that Phaffia rhodozyma is not haploid. Auxotrophic strains were generated at a high frequency following treatment of mutagenized cells with a combination
of benomyl and ethyl acetate. Studies of an auxotrophic mutant using flow cytometry and UV inactivation indicated possible
chromosome loss to an aneuploid state.
Received 21 June 1998/ Accepted in revised form 25 September 1998 相似文献