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巨噬细胞集落刺激因子与动脉粥样硬化欧阳谦,周玫,陈瑗(第一军医大学自由基医学研究室,广州510515)关键词巨噬细胞集落刺激因子,氧化修饰低密度脂蛋白,动脉粥样硬化巨噬细胞集落刺激因子(MCSF)是一种多肽类激素样造血生长因子,可由单核细胞、巨噬细胞... 相似文献
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目的:开发一种粒细胞-巨噬细胞集落刺激因子(GM—CSF)长效缓释微球剂型。方法:采用S/O/hO法制备了包裹粒细胞一巨噬细胞集落刺激因子多糖玻璃体颗粒的PLGA微球,考察了微球的表面形态、粒径分布等,并且运用ELISA方法考察了微球的体外释放效果。结果:本方法制备的粒细胞-巨噬细胞集落刺激因子微球光滑圆整,粒径分布均匀,体外可以缓释达32天,累积释放率接近90%。结论:本方法制备的粒细胞-巨噬细胞集落刺激因子微球能有效地保护蛋白活性,同时实现长效缓释的目标,是一种可行的蛋白缓释方案。 相似文献
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研究了重组人粒细胞集落刺激因子突变体(rmhG-CSF)的聚乙二醇化修饰、分离纯化和活性鉴定。通过对人重组粒细胞集落刺激因子(rhG-CSF)第1,3,4,5,17位氨基酸进行突变,并在C末端加了一个半胱氨酸,获得了体外活性为原型rhG-CSF 150%以上的重组人粒细胞集落刺激因子突变体(rmhG-CSF)。然后用分子量为20kD的甲氧聚乙二醇马来酸酐(mPEG-Mal)修饰rmhG-CSF,反应混合物经离子交换和凝胶过滤柱纯化,得到纯化的聚乙二醇重组人粒细胞集落刺激因子突变体(PEG-rmhG-CSF)。SDS-PAGE电泳分析表明纯化后的PEG-rmhG-CSF的纯度大于95%,体外活性分析表明PEG-rmhG-CSF活性优于目前临床使用的聚乙二醇重组人粒细胞集落刺激因子(PEG-rhG-CSF,Neulasta?),药代动力学研究表明PEG-rmhG-CSF体内半衰期约为14h,比修饰前延长了7倍。 相似文献
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培养大鼠主动脉血管平滑肌细胞产生巨噬细胞集落刺激因子及其受体的表达 总被引:1,自引:0,他引:1
本研究用培养大鼠主动脉血管平滑肌细胞(VSMCs),结果如下:(1)用生物活性检测法发现VSMCs无血清条件培养液可刺激巨噬细胞集落形成,其作用能被抗巨噬细胞集落刺激因子(MCSF)抗体抑制;(2)用免疫细胞化学技术证明VSMCs存在MCSF受体;(3)用Northern blot技术证明VSMCs有MCSF及其受本的mRNA表达,血清刺激使两者表达明显增强。本研究首次报道了培养大鼠主动脉VSMC 相似文献
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FL对脐血造血细胞长期液体培养的影响 总被引:3,自引:0,他引:3
用脐血进行干细胞移植有许多优点,但有一个主要的缺点是可获得的细胞数量有限,因此脐血干细胞的体外扩增对于其临床应用具有重要意义。考察了Flt-3配体(FL)和干细胞因子(SCF),白介素3(IL-3),IL-6,粒细胞集落刺激因子(G-CSF),粒细胞巨噬细胞集落刺激因子(GM-CSF)的组合对脐血细胞扩增和分化的影响,培养42d,总细胞最多扩增了385.30±163.51倍(FL+SCF+G-CS 相似文献
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对重组人粒细胞-巨噬细胞集落刺激因子(rhGM-CSF)的工程菌表达产物进行纯化,经过超声破碎,包涵体抽提,凝胶层析,复性,离子交换一系列纯化步骤,终产物纯度达98%,比活性达10000000u/mg。 相似文献
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对重组人粒细胞-巨噬细胞集落刺激因子(rhGM-CSF)的工程菌表达产物进行纯化,经过超声破碎,包涵体抽提,凝胶层析,复性,离子交换一系列纯化步骤,终产物纯度达98%,比活性达10000000u/mg。 相似文献
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非转座子载体介导的稳定转化家蚕BmN细胞表达人粒细胞-巨噬细胞集落刺激因子 总被引:2,自引:0,他引:2
为了建立非转座子载体介导的持续表达外源基因的转化家蚕BmN细胞系,将家蚕核型多角体病毒极早期基因(ie-1)启动子控制的人粒细胞-巨噬细胞集落刺激因子(hGM-CSF)基因的表达盒克隆至pIZT/V5-His,获得重组载体pIZT-IE-hGM-CSF,该载体转染家蚕BmN细胞后,通过博莱霉素(Zeocin)筛选获得了稳定转化细胞系IE-hGM-CSF。转基因细胞基因组经PCR鉴定,成功检测到ie-hGM-CSF,Western blotting分析结果显示转化细胞表达的重组hGM-CSF的大小为22kDa,ELISA检测结果显示hGM-CSF在转化细胞系里的表达水平大约为2814.7pg/106个细胞。 相似文献
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Recent advances in the study of Kaposi's sarcoma-associated herpesvirus replication and pathogenesis
It has now been over twenty years since a novel herpesviral genome was identified in Kaposi's sarcoma biopsies. Since then, the cumulative research effort by molecular biologists, virologists, clinicians, and epidemiologists alike has led to the extensive characterization of this tumor virus, Kaposi's sarcoma-associated herpesvirus(KSHV; also known as human herpesvirus 8(HHV-8)), and its associated diseases. Here we review the current knowledge of KSHV biology and pathogenesis, with a particular emphasis on new and exciting advances in the field of epigenetics. We also discuss the development and practicality of various cell culture and animal model systems to study KSHV replication and pathogenesis. 相似文献
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正Dear Editor,In December 2019, a novel human coronavirus caused an epidemic of severe pneumonia(Coronavirus Disease 2019,COVID-19) in Wuhan, Hubei, China(Wu et al. 2020; Zhu et al. 2020). So far, this virus has spread to all areas of China and even to other countries. The epidemic has caused 67,102 confirmed infections with 1526 fatal cases 相似文献
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Curcumin is the yellow pigment of turmeric that interacts irreversibly forming an adduct with thioredoxin reductase (TrxR), an enzyme
responsible for redox control of cell and defence against oxidative stress. Docking at both the active sites of TrxR was performed to compare
the potency of three naturally occurring curcuminoids, namely curcumin, demethoxy curcumin and bis-demethoxy curcumin. Results show
that active sites of TrxR occur at the junction of E and F chains. Volume and area of both cavities is predicted. It has been concluded by
distance mapping of the most active conformations that Se atom of catalytic residue SeCYS498, is at a distance of 3.56 from C13 of
demethoxy curcumin at the E chain active site, whereas C13 carbon atom forms adduct with Se atom of SeCys 498. We report that at least
one methoxy group in curcuminoids is necessary for interation with catalytic residues of thioredoxin. Pharmacophore of both active sites of
the TrxR receptor for curcumin and demethoxy curcumin molecules has been drawn and proposed for design and synthesis of most probable
potent antiproliferative synthetic drugs. 相似文献
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RICHARD E. NORRIS 《Botanical journal of the Linnean Society. Linnean Society of London》1991,106(1):1-40
Comprises species occurring mostly in subtidal habitats in tropical, subtropical and warm-temperate areas of the world. An analysis of the type species, V. spiralis (Sonder) Lamouroux ex J. Agardh, a species from Australia, establishes basic characters for distinguishing species in the genus. These characters are (1) branching patterns of thalli, (2) flat blades that may be spiralled on their axis, (3) width of the blade, (4) primary or secondary derivation of sterile and fertile branchlets and (5) position of sterile and fertile branchlets on the thalli. Application of the latter two characters provides an important basic method for separation of species into three major groups. Osmundaria , a genus known only in southern Australia, was studied in relation to Vidalia , and its separation from the Vidalia assemblage is not accepted. Species of Vidalia therefore are transferred to the older genus name, Osmundaria. Two new species, Osmundaria papenfussii and Osmundaria oliveae are described from Natal. Confusion in the usage of the epithet, Vidalia fimbriala Brown ex Turner has been clarified, and Vidalia gregaria Falkenberg, described as an epiphyte on Osmundaria pro/ifera Lamouroux, is revealed to be young branches of the host, Osmundaria prolifera. 相似文献
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JOAN VALUÈS MONTSERRAT TORRELL NÚRIA GARCIA JACAS 《Botanical journal of the Linnean Society. Linnean Society of London》2001,137(4):399-407
Fifteen chromosome counts of six Artemisia taxa and one species of each of the genera Brachanthemum, Hippolytia, Kaschgaria, Lepidolopsis and Turaniphytum are reported from Kazakhstan. Three of them are new reports, two are not consistent with previous counts and the remainder are confirmations of very scarce (one to four) earlier records. All the populations studied have the same basic chromosome number, x = 9, with ploidy levels ranging from 2x to 6x. Some correlations between ploidy level, morphological characters and distribution are noted. 相似文献