共查询到20条相似文献,搜索用时 31 毫秒
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The structural organization of the chicken calmodulin gene 总被引:13,自引:0,他引:13
R C Simmen T Tanaka K F Ts'ui J A Putkey M J Scott E C Lai A R Means 《The Journal of biological chemistry》1985,260(2):907-912
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We have isolated two allelic duck-genomic DNA fragments containing beta-type globin genes. The beta- and epsilon-globin genes are 1800 bp apart in both fragments, have second introns of 1100 and 1200 bp, respectively, and small first introns. We have also determined approx. 550 nucleotides of the sequence on the 5' side of the beta-globin gene. Comparison with the chicken beta-globin gene suggests sequences of possible significance to gene expression. 相似文献
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Isolation and functional analysis of chicken 90-kDa heat shock protein gene promoter. 总被引:2,自引:1,他引:1 下载免费PDF全文
C Vourc'h N Binart B Chambraud J P David V Jrme E E Baulieu M G Catelli 《Nucleic acids research》1989,17(13):5259-5272
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The entire nucleotide sequence of the chicken cardiac alpha-actin (CC alpha A) gene has been determined. This is the first complete sequence of a cardiac actin gene that includes the promoter region, cap site, all the introns, and the polyadenylation site. The gene contains six introns, five of which interrupt the coding region at amino acids (aa) 41, 150, 204, 267, and 327. The first intron is in the 5'-noncoding region and is 438 bp in length. The CC alpha A gene encodes an mRNA of approx. 1400 bp with 5'- and 3'-untranslated region of 59 and 184 nucleotides (nt), respectively. Like the chicken skeletal alpha-actin gene, the CC alpha A gene has the codon for the aa cysteine between the initiator ATG and the codon for the N-terminal aspartic acid residue of the mature protein. There are no strong homologies (less than 13 consecutive nt) in the promoter or 3'-untranslated regions between the CC alpha A and chicken skeletal alpha-actin genes even though both are expressed in skeletal muscle during development. However, the 3'-untranslated region of the CC alpha A gene demonstrates significant sequence homology (76% over a 200-nt region) with the same region in the partial sequence of the human cardiac gene. The conservation of these sequence homologies between identical isoforms rather than the different alpha actin genes suggests these conserved regions may have a role in regulation rather than tissue-specific expression, as previously proposed. 相似文献
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A phase relationship associates tRNA structural gene sequences with nucleosome cores 总被引:17,自引:0,他引:17
DNA (760 bp) isolated from nucleosome tetramers of staphylococcal nuclease-digested chicken embryo chromatin was highly enriched for tRNA genes and subsequently cloned in E. coli chi 1776. The location of genes coding for chicken embryo tRNALys, tRNAPhe and tRNAiMet within the cloned nucleosome tetramer DNA was determined using restriction endonucleases for which single cleavage sites could be predicted from the respective tRNA base sequence. All our tRNA genes reside nonrandomly at four locations on nucleosome tetramer DNA. The spacing between the tRNA gene locations is approximately 190 bp, similar to the DNA repeat length of chicken embryo chromatin. The four tRNA gene locations were also defined in noncloned nucleosome tetramer DNA highly enriched for tRNA genes. The majority of genes coding for tRNALys, tRNAPhe and tRNAiMet, respectively, are located in equal proportion 40-45, 230, 420 and 610 bp distant from the 5' end of the tRNA-identical strand. Thus the tRNA structural gene sequences all appear to begin about 20 bp "inside" the nucleosome core. As observed with nucleosomal DNA not enriched for tRNA genes, the phase relationship between tRNA genes and nucleosome location is maintained over a distance of 4-6 subsequent nucleosomes. A cloned molecule of nucleosomal DNA containing both a tRNALys gene and a tRNAiMet gene in the same polarity reveals that a phase adjustment might be necessary for the nucleosomes between these two tRNA genes in chicken embryo chromatin. 相似文献
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Chromosomal arrangement of the duck alpha-globin genes and primary structure of the embryonic alpha-globin gene pi 总被引:1,自引:0,他引:1
A recombinant lambda Charon 4A bacteriophage, D alpha G-1, carrying the genes coding for the duck embryonic (pi') and adult (alpha A, alpha D) alpha-like globins was isolated from a previously constructed duck DNA recombinant library. The three globin genes are transcribed from the same DNA strand and are arranged in the order of their expression during development: 5'-pi'-alpha D-alpha A-3'. We have determined the complete nucleotide sequence of the duck pi'-globin gene, including the flanking regions. Due to the unusual length of intron 1 (963 bp) and intron 2 (568 bp) the 2167-bp duck pi'-globin gene is by far the largest among all known mammalian or avian alpha- and beta-globin genes. For instance, the duck pi'-globin gene introns are almost twice as long as those of the chicken pi'-globin genes. A surprisingly high degree of nucleotide sequence homology (88%) has been found for the 5' flanking region (positions -1 to -223) of the duck and chicken pi'-globin gene. 相似文献
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M Romana A Dubart D Beaupain C Chabret M Goossens P H Romeo 《Nucleic acids research》1987,15(18):7343-7356
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The structure and complete nucleotide sequence of the avian lipoprotein lipase gene. 总被引:6,自引:0,他引:6
D A Cooper S C Lu R Viswanath R N Freiman A Bensadoun 《Biochimica et biophysica acta》1992,1129(2):166-171
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Structure of the rat L-type pyruvate kinase gene 总被引:10,自引:0,他引:10