Effect of zinc-amended poultry manure and zinc sulphate on the growth and uptake of zinc by corn (Zea mays L.)

Summary A greenhouse experiment conducted to study the effect of different levels of Zn supplied through Zn-amended poultry manure and ZnSO₄ on corn (Zea mays L.) indicated that both the sources significantly increased the dry matter yield and uptake of zinc. The zinc amended poultry manure at all levels of zinc application was more effective than ZnSO₄ in this respect. The percent zinc in corn derived from the fertilizer and percent utilization of applied zinc from the Zn-amended poultry manure was higher than that from ZnSO₄ when applied at equivalent levels.     

Aerobic heterotrophic bacteria and petroleum-utilizing bacteria from cow dung and poultry manure

In this study, enumeration and identification of total aerobic heterotrophic bacteria and petroleum-utilizing bacteria as well as the degradative potential of petroleum-utilizing bacterial isolates were carried out. The average counts of total aerobic heterotrophic bacteria in cow dung and poultry manure were 74.25 × 10⁵ c.f.u. g⁻¹ and 138.75 × 10⁵ c.f.u. g⁻¹ respectively. Acinetobacter sp, Bacillus sp, Pseudomonas sp, and Serratia spp. occurred as aerobic heterotrophs in both cow dung and poultry manure. However, Alcaligenes spp. occurred only in cow dung while, Flavobacterium sp, Klebsiella sp, Micrococcus sp, and Nocardia spp. occurred only in poultry manure as aerobic heterotrophs. The average counts of petroleum-utilizing bacteria in cow dung and poultry manure were 9.25 × 10⁵ c.f.u. g⁻¹ and 17.25 × 10⁵ c.f.u. g⁻¹ respectively. Pseudomonas spp. occurred as petroleum utilizer in both cow dung and poultry manure. However, Bacillus spp. occurred only in cow dung while Acinetobacter spp. and Micrococcus spp. occurred only in poultry manure as petroleum utilizers. Relative abundance of petroleum utilizers in total aerobic heterotrophs ranged from 6.38% to 20.00% for cow dung and from 9.38% to 17.29% for poultry manure. Introduction of pure cultures of petroleum-utilizing bacteria from cow dung and poultry manure into sterile oil-polluted soil revealed oil degradation in one week period.     

Enhancement of resistance by poultry manure and plant hormones (salicylic acid & citric acid) against tobacco mosaic virus

Virus is the most menacing factor for plant, which causes enormous economic losses in agriculture worldwide. Tobacco mosaic virus is most hazardous virus among the plants that can spread through biological and non-biological sources. TMV is ancient virus that causes huge economic losses to pepper cucumber ornamental crops and tobacco. It can be controlled by reducing the population of vector through pesticide application. However, the rapid usage of synthetic chemicals causes environmental pollution and destroys our ecosystem. Consequently, different approaches just like natural derivatives should be adopted for the environmental friendly management for TMV. This in vitro study demonstrated the potential role of natural metabolites such as poultry manure and plant extracts such as salicylic acid and citric acid for the control of TMV. Two different concentrations of poultry manure 60G and 30G were used. Poultry manure was mixed with the soil at the time of sowing. Disease severity was minimum at maximum concentration as compared to the control. Meanwhile, two different concentrations of salicylic acid and citric acid 60% and 90% were applied by foliar sprayer after three-leaf stages. Disease severity was observed after 5, 10, 15, 20, 25, and 30 days after disease inoculation. Here also maximum concentration showed the minimum disease severity and higher concentration of both animal and plants extracts were used for following experiment. Quantitative real-time PCR (RT-qPCR) results demonstrated that different plant defense-related genes such as PR1a, PAL, PR5, NPR1, PRIb, and PDF1.2 were up-regulated. Furthermore, applications of each treatment-induced systemic resistance against a wide range of pathogen including TMV and fungal pathogen Botrytis cinerea.     

Composting of poultry manure and wheat straw in a closed reactor: optimum mixture ratio and evolution of parameters

The main objectives of this work were to investigate the evolution of some principal physico-chemical properties (temperature, carbon dioxide, oxygen, ammonia, pH, electrical conductivity, organic matter) and microbial population (mesophilic and thermophilic bacteria and fungi) during composting poultry manure with wheat straw in a reactor system, and to evaluate the optimum mixture ratio for organic substrate production. The experiments were carried out in four small laboratory reactors (1 l) and one large reactor (32 l) under adiabatic conditions over 14 days. During the process the highest temperature was 64.6°C, pH varied between 7.40 and 8.85, electrical conductivity varied between 3.50 and 4.31 dS m⁻¹ and the highest value of organic matter (dry weight) degradation was 47.6%. Mesophilic bacteria and fungi predominated in the beginning, and started the degradation with generation of metabolic heat. By increasing the temperature in reactors, the number of thermophilic microorganisms also increased, which resulted in faster degradation of substrate. The application of a closed reactor showed a rapid degradation of manure/straw mixture as well as a good control of the emissions of air polluting gases into atmosphere. The results showed that the ratio of manure to straw 5.25:1 (dry weight) was better for composting process than the other mixture ratios.     

生物炭影响作物生长及其与化肥混施的增效机制研究进展

利用秸秆型生物炭进行还田改土不仅具有提升作物产量的潜力,而且能够产生明显的环境效益,现已成为当今国内外农业领域的研究热点.本文综述了近年来国内外有关生物炭添加影响作物生长的分子调控机制研究,尤其关注了生物炭与作物根系的互作效应;介绍了生物炭与化肥混施的生物学效应及可能的增效机制;展望了今后的研究方向,以期促进我国相关领域的研究.国内外的最新研究表明:生物炭土壤添加改善植物生长的关键是生长素相关信号转导分子,通过促进植物细胞扩增、细胞壁松弛、水及营养的转运等相关基因的表达,有利于植物的新陈代谢及生长.生物炭及其与根系的相互作用能够直接或间接地影响土壤物理、化学、生物因子,从而在炭、肥互作增效过程中起主导调控作用.     

添加竹炭对猪粪堆肥过程中升温脱水及氮素损失的影响

堆肥是实现畜禽粪便处理及资源化利用的有效途径,但传统堆肥过程存在升温脱水效果不佳及氮素损失的问题.本文利用猪粪进行堆肥试验,探讨了堆肥过程中添加不同比例竹炭对堆肥升温脱水及氮素损失的影响.结果表明: 与不添加竹炭的对照相比,添加竹炭处理可以使堆体升温时间缩短24～48 h,脱水率提高13.6%～21.4%,堆肥高温期持续时间延长216～264 h;添加竹炭处理可以增加堆肥铵态氮、硝态氮及总氮含量,使氮素固定率提高28.3%～65.4%.
     

Investigating the response of soil and vegetable crops to poultry and cow manure using ground and satellite data

Based on the massive production of cow and poultry manures, farmers in Saudi Arabia are moving towards the application of organic fertilizers in their farms. Therefore, the present work was conducted to study the response of soil and selected vegetable crops to poultry and cow manures, using ground data and Landsat-8 and Hyperion images. The studied vegetable crops are cabbage, cauliflower, broccoli, and lettuce. A total of 100 t ha^?1 organic manures were applied as a pre-planting treatment. A 12.5 ha field in Tawdeehiya Farms, 200 km southeast of Riyadh, was earmarked for this study. The field was divided into sectors cultivated with the above-mentioned vegetable crops. Soil characteristics, including the soil pH, the electric conductivity (EC), the nitrogen (N), the phosphorus (P) and the potassium (K), were examined before the application of manures and 25 days after the transplanting process. Observations on crops chlorophyll content, number of leaves, the diameter of merchantable products and yield were also investigated. Furthermore, the relationship between the crop performance and yield was investigated through the satellite images generated vegetation indices (VIs). This study revealed the better performance of poultry manure compared to cow manure in terms of development and production parameters of the experimental crops. Dynamics of the chlorophyll content across the crop growth period revealed that all the tested crops responded significantly (R² = 0.69; P = 0.001) to the poultry manure treatments. Among the tested crops, the chlorophyll content, curd or head sizes and crop yields were quite better in poultry manure applied plots. The investigation of crop yield was significant with poultry manure (R² = 0.64; P = 0.001) than cow manure (R² = 0.57; P = 0.001) using the OSAVI and mNDVI, respectively.     

Stimulation of hybrid poplar growth in petroleum-contaminated soils through oxygen addition and soil nutrient amendments

Hybrid poplar trees (Populus deltoides x nigra DN34) were grown in a green-house using hydrocarbon-contaminated soil from a phytoremediation demonstration site in Health, Ohio. Two independent experiments investigated the effect of nutrient addition on poplar growth and the importance of oxygen addition to root development and plant growth. Biomass measurements, poplar height, and leaf color were used as indicators of plant health in the selection of a 10/5/5 NPK fertilizer applied at 1121 kg/ha (112 kg-N, 24.4 kg-P, 46.5 kg-K per ha) to enhance hybrid poplar growth at the Health site. Five passive methods of oxygen delivery were examined, including aeration tubes, gravel addition, and an Oxygen Release Compound (ORC). When ORC was placed in coffee filters above hydrocarbon-contaminated soil, a statistically significant increase of 145% was observed in poplar biomass growth, relative to unamended controls. The ORC in filters also stimulated significant increases in root density. A 15.2-cm interval of soil directly below ORC addition exhibited an increase from 2.6 +/- 1.0 mg/cm3 to 4.8 +/- 1.0 mg/cm3, showing stimulation of root growth in hydrocarbon-stained soil. The positive response of hybrid poplars to oxygen amendments suggests that overcoming oxygen limitation to plants should be considered in phytoremediation projects when soil contamination exerts a high biochemical oxygen demand, such as in former refinery sites.     

Recovery of activated carbon catalyst,calcium, nitrogen and phosphate from effluent following supercritical water gasification of poultry manure

A new method for suspending a fine, activated carbon catalyst in a biomass feedstock used for a supercritical water gasification (SCWG) continuous reactor is proposed. In a previous study, the organic matter in poultry manure was shown to be completely converted into gases such as H₂, CO₂ and CH₄ using SCWG. In practice, however, since the feedstock is not only composed of organic matter, but also contains inorganic material, water and catalyst, products such as gas, solid and liquid are produced during SCWG. The aim of this work was to investigate SCWG by-product utilization. This paper describes fundamental studies on the recovery of materials from SCWG products to develop a novel and simple recycling process that utilizes the by-products. A major portion of the activated carbon, monetite and ammonium sulfate can be isolated from the SCWG effluent.     

Ecological effects of cow manure compost on soils contaminated by landfill leachate

The experiment was conducted to evaluate the effect of cow manure compost (CMC) application on leaching toxicity of leachate polluted soils by using Tetrahymena pyriformis (TP). Soils treated with various levels of leachate (0, 12.5 ml, 25 ml, 37.5 ml, and 50 ml leachate per 300 g soil) were amended with 0, 25 g and 50 g CMC, respectively. The results showed CMC application resulted in 7–18% lower leaching toxicity while excessive CMC has no significant benefit for decreasing leaching toxicity further. The alleviating effect of CMC on biotoxicity of soil extract was mainly attributed to either pH increase, high content of P and organic matter, or promotion on soil microbial metabolism and especially pH played an important role in alleviating effect. And the observations indicated that death rate (DR) of TP was more sensitive to leachate level respect to other biological parameters above and TP was effective as the test organism for leaching toxicity. Further studies are needed to unambiguously determine in-deep mechanism of toxicity impacts on TP posed by leachate pollutants.     

The impact of corncob biochar and poultry litter on pepper (Capsicum annuum L.) growth and chemical properties of a silty-clay soil

Red pepper (Capsicum annuum L.) is one of the most commonly cultivated vegetable in the Mediterranean region. This study evaluated the effects of biochar derived from corncob and poultry litter on growth of red pepper (Capsicum annuum L.) and some chemical properties of a silty clay soil. The experiment consisted of two factors, i.e., biochar doses (0, 0.5, 1.0 and 2%) and poultry litter doses (0, 0.5, 1.0 and 2%). The number of days to 50% flowering, plant height, stem diameter, total number of leaves per plant, the number of main branches per plant, fresh root weight, root length, dry shoot weight, macro (P and K) and micro (Fe, Zn, Cu and Mn) nutrient concentrations of leaves were determined to compare the efficiency biochar and poultry litter. Moreover, post-harvest soil analysis was conducted to measure pH, organic matter, and macro and micronutrient contents. Biochar had varying impact on plant growth parameters, whereas poultry litter alone or in combination with biochar increased macro and micronutrient concentrations of soil and improved most of the growth parameters of red pepper. In contrast, sole biochar application had no significant impact on most of the growth parameters. Wider C/N ratio (107.7) of corncob derived biochar restricted the nitrogen supply for plant growth. The combination of 0.5% biochar and 2% poultry litter resulted in the highest plant height (36.7 cm) and stem diameter (0.69 cm). The results revealed that application of single biochar derived from corncob is insufficient to supply adequate nutrients for optimal plant growth. The application of biochar alone enhances carbon sequestration in soils, however most biochars like cornconb biochar do not contain sufficient available plant nutrients. Therefore, biochars should be applied along with mineral fertilizers or organic materials such as poultry manure which is rich in available plant nutrients.     

Heavy metal accumulation in wheat and barley: The effects of soil presence and liquid manure amendment

An experiment was undertaken to evaluate the effect of liquid manure amendment on heavy metal accumulation in wheat and barley. For this purpose, both kinds of seedlings were grown simultaneously in a Petri dish, while wheat seedlings were also grown in pots containing unpolluted agricultural soil. All of the seedlings were irrigated with one of the three prepared solutions: artificial rainwater solution, heavy metal solution and liquid manure solution containing NH₄NO₃, H₃PO₄ and KOH along with equal amounts of heavy metals as in the second solution. Twenty days later, 1 g of plant tissue was digested with the mixture of HNO₃ and H₂O₂ for ICP-OES/HG-ICP-OES analysis. The results showed that the uptake of arsenic and mercury was highest for both plants grown in a Petri dish. Furthermore, the wheat grown in a Petri dish also had a high content of nickel, cadmium and copper, while the pot-grown wheat contained high amounts of iron and manganese, probably due to the adsorption of nickel, cadmium, copper and mercury on soil phases. The lower uptake of all heavy metals was observed after the amendment of liquid manure, with the exception of manganese in wheat and mercury in all plants.     

生物炭对猪粪堆肥过程中细菌群落结构的影响

【背景】细菌群落多样性影响堆肥过程,生物炭影响细菌的生长,但生物炭对猪粪堆肥细菌群落结构的影响尚未见报道。【目的】根据细菌群落结构和堆肥温度的变化规律,添加适量的生物炭到猪粪堆肥,以提高堆肥主要细菌的占比和堆肥效率,为推广生物炭和猪粪堆肥的联合应用提供参考。【方法】设计0%、3%、6%和9%的生物炭添加量,在堆肥的高温期和温度稳定期分别取样,总共8个样品。根据IlluminaMiSeq对细菌16SrRNA基因的高通量测序结果,分析生物炭添加量和堆肥温度对猪粪堆肥细菌群落结构的影响。【结果】在细菌门水平上,猪粪堆肥主要的细菌有Proteobacteria、Actinobacteria、Bacteroidetes、Chloroflexi、Gemmatimonadetes、Firmicutes、Acidobacteria和Deinococcus-Thermus;在细菌属水平上,猪粪堆肥主要的细菌有Chryseolinea、Subgroup_6_norank、Steroidobacter、Anaerolineaceae、Nonomuraea、Longispora、Bacillus、Sporacetigenium、Luteimonas、Phyllobacteriaceae、Truepera、Rhodothermacea和Aquamicrobium。生物炭添加量对猪粪堆肥主要细菌的生长既有促进作用,也有抑制作用。随着生物炭添加量的增加,Bacillus、Streptomyces、Rhodothermaceae和Firmicutes的丰度随之增加,而Chryseolinea、Longispora和Steroidobacter的丰度却随之减少。Firmicutes、Bacillus和Streptomyces的丰度在堆肥高温期要大于堆肥温度稳定期,而Chloroflexi、Anaerolineaceae和Longispora则相反。猪粪堆肥高温期的细菌多样性要明显大于堆肥温度稳定期。堆肥高温期的细菌群落达到70个,远多于堆肥温度稳定期的15个;其中对猪粪堆肥起主要作用的细菌,堆肥高温期时达到7个(Rhizobiales、Incertae_Sedis、Proteobacteria、Alphaproteobacteria、Xanthomonadales、Gammaproteobacteria和Steroidobacter),而堆肥温度稳定期只有3个(Micromonosporales、Longispora和Micromonosporaceae)。猪粪堆肥添加生物碳后,环境因子(电导率、含水量、温度和pH)对堆肥主要的细菌不能产生显著的影响。β-Proteobacteria、Rhodothermaceae、Phyllobacteriaceae和Bacterium是含水量、温度和pH影响最大的细菌。【结论】生物碳添加量和堆肥温度能改变猪粪堆肥的细菌群落结构,在猪粪堆肥高温期能显著增加细菌的数量和多样性。猪粪堆肥的电导率、含水率、温度和pH能影响堆肥细菌的生长,但对堆肥主要细菌的影响不显著。     

Influences of rice straw biochar and organic manure on forage soybean nutrient and Cd uptake

Abstract

This pot experiment aimed to investigate the influence of rice straw biochar (BC 0, 1, and 3%, w/w) and organic manure (OM 0, 1, and 2%, w/w) addition on the growth, nutrient and cadmium (Cd) uptake of forage soybean in 10 mg Cd kg^?1 contaminated soils. Compared with non-biochar treatments, biochar decreased shoot biomass, height and nitrogen (N) contents. Organic manure markedly increased the shoot biomass, shoot phosphorus (P), potassium (K), calcium (Ca) and magnesium (Mg) concentration, and root N, P, Ca contents without biochar addition treatments, while in the case of 3% biochar, there were no significant effects on N, K, Ca, and Mg contents of shoot and root among organic manure treatments. In comparison with other treatments, the minimum Cd content of shoots and roots both occurred in the treatment of BC3%+OM2%, while shoot Cd content reached the maximum value in OM2% treatment. Thus, these results suggested that organic manure addition can elevate forage soybean yield and nutrient content, while biochar had no positive effects. High biochar (3%) addition in combination with highest dose of organic manure (2%) can decline the Cd content of soybean and contribute to the agricultural product safety.     

Effect of long-term application of animal manure on physical properties of three soils

Surface soil samples to 15 cm depth were taken from replicated plots in an ongoing long-term field experiment involving application of animal manure on three soils in Virginia. The sampled plots had received either no manure or the equivalent of 289,000 kg ha^–1 of manure as dry weight. The manure was applied annually at the beginning of each spring for 15 years from 1978 through 1992. The plots were cropped similarly since 1978. Soil textures were a fine sandy loam at Holland in the Atlantic Coastal Plain region, a silt loam at Blacksburg in the Appalachian region, and a clay loam at Orange in the Piedmont region of Virginia. The following measurements were made on subsamples: liquid and plastic limits, wet aggregate stability, aggregate size distribution, dispersible clay percentage, water retention at 0. 03, 0.1, 0.3, 0.5, 1.0, and 1.5 MPa tension, and modulus of rupture of moulded briquettes at a water content corresponding to 0.1 MPa tension. Organic matter content by the Walkley-Black method was significantly higher in the manure-treated soils at all three locations. Increases were 3% for the sandy loam and 25% for the silt loam and clay loam. From these values it was estimated that at least 95% of the total applied manure had been degraded over the 15 years. Results showed that the liquid and plastic limits for all three soils were higher (p<0.05) for the manure-treated samples. However, the differences in the limits were only 2 to 3%. The modulus of rupture values were lowered by addition of the animal manure. Decreases (p<0.05) occurred for the silt loam and clay loam samples. The wet aggregate stability increased and the dispersible clay decreased in the manure-treated soils. Increases (p<0.05) in wet aggregate stability occurred for the sandy loam and silt loam samples. Decreases (p<0.05) in dispersible clay were measured for the sandy loam and clay loam samples. Water retention was consistently, but only slightly, increased by manure addition. The increases, in the order of sample texture, were clay loam > sandy loam silt loam. Increases tended to be higher at the lower values of tension. Manure addition consistently increased the weight percentages of aggregates passing a given mesh size. Increases, in order of sample texture, were silt loam > clay loam > sandy loam. In their entirety, these results show that the manure produced measurable changes in the soil physical properties. The magnitude of the changes, in most cases, were small and depended on the soil texture. Given the high total amount of manure applied, the results indicate that manure-induced physical changes in the soil were small and evidently did not accumulate over time. Rapid microbial degradation of the manure could be responsible for the lack of marked changes in the soil physical properties.     

凝结芽胞杆菌生物学特性及其在家禽生产中的应用

作为一种新兴的微生物饲料添加剂,凝结芽胞杆菌既表现出类乳酸菌的益生特性,又具有芽胞杆菌抗逆性好、易储存的优点,有利于产业化及市场推广。凝结芽胞杆菌能够产生有机酸及多种酶类、修复受损的肠黏膜、抑制病原菌繁殖及促进营养物质的消化吸收,在家禽生产中受到广泛关注和应用。目前关于凝结芽胞杆菌的研究不够深入,其生物学特性及作用机制还在进一步研究中,还需加深其在畜牧业中的应用研究。本文主要对凝结芽胞杆菌近几年的研究进展及其在家禽生产中的应用进行综述,为今后凝结芽胞杆菌的研发及应用提供参考依据。     

Effect of poultry manure on the growth,yield and root-knot nematode (Meloidogyne spp.) infestation of carrot (Daucus carota L.)

Currently, synthetic pesticides which are highly detrimental to man and the environment are the principal means of nematode control. However, the use of organic amendments might provide a sustainable control option as nematicidal properties have been identified in many animal wastes. A field experiment was carried out to determine the effectiveness of different levels of poultry manure (PM) applications on root-knot nematode infestation of carrot. There were four treatments (0, 2, 4 and 6?t/ha of PM) laid out in a randomised complete block design with four replications. The various levels of PM were worked into the soil on raised beds before carrot seeds were sown. Data were taken on growth performance (plant height, number of leaves and canopy spread), yield characteristics (root weight, root length and root girth) and infestation (root-galling index and nematode population in the soil). PM significantly reduced both root galling and nematode population with 4?t/ha significantly increasing yield characters of carrot. Since PM significantly reduced (p?<?0.05) nematode infestation and promoted growth and yield of carrot, farmers are encouraged to use it preferably at 4?t/ha.     

Organic manure additions and the leaf water potential and yield of barley

The effect of adding organic manure to three soils with differing textures on changes in leaf water potential (LWP) and yield of barley (cv. Loyola) was investigated under controlled growth-chamber conditions. Cattle manure was applied to the soils in pots at rates equivalent to 0, 15, 25, 50 and 100 t ha^-1. Plants were subjected to water stress by withholding water at three different stages of grwoth.The results show that the addition of cattle manure significantly increased (p<-0.05) the organic carbon (OC) content of all the soils. LWP remained consistently high during the entire growing period in the control plants. As stress progressed, LWP in the stressed plants decreased. However, treatments with high OC contents had significantly higher LWP compared to those which had less. The former plants experienced less water stress than the latter.Yields were higher in the control than stressed plants. Within the stressed plants however, treatments with high OC content had significantly higher yields. An effect of soil texture was also noted. Generally, the influence of manure application on LWP and yields were more pronounced in sand and loam than clay soils.     

利用EM和OL处理猪粪的效果研究

The effects of EM and OL on the color,pHvalue,stink and the various kinds of harmful microbes of pig manure were analyzed.The results show that EM and OL could significantly reduce the stink and restrain the number of harmful microbes.At the same time,the rotting process of pig manure was accelerated,and the number of flies around pig manure reduced.     

施用生物炭对土壤动物群落的影响研究进展

土壤动物是土壤生物群落不可或缺的组成部分,是调控土壤生态过程重要的生物驱动因子。探明向土壤中施加生物炭对土壤动物群落的影响及二者之间的相互关系,对深刻认识土壤生态系统的运行机制、评价土壤生态服务功能具有重要意义。本文综述了施用生物炭对土壤动物群落的影响及机制,包括生物炭原料、制备温度、施用量的差异对土壤动物群落造成的直接影响,及以生物介导(改变植物生理特性、提高微生物数量)和非生物介导(土壤理化性质的改变)环境条件的改变对土壤动物群落造成的间接影响。低量生物炭添加下(生物炭与土壤质量比＜5%),对土壤动物的生长繁殖和行为活动起促进作用,若施炭量过高(＞10%),则会产生毒害;土壤动物的行为活动也会影响生物炭的稳定性。未来应该加强长期田间定位、时空变异性、多学科交融和分析预测等方面的研究。