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1.
The southern armyworm (SAW) Spodoptera eridania (Cramer) is one of the most common armyworm species defoliating soybeans. Preliminary screening trials have indicated that some soybean genotypes exhibit resistance to SAW. Therefore, in this study, we evaluated the development of SAW larvae fed on ten soybean genotypes in order to identify genotypes with antibiosis-type resistance. Neonate SAW larvae were daily fed with young leaves collected from plants at the vegetative growth stages V4–V5. Larval development and survival were recorded. Genotypes PI 227687 and PI 227682 delayed larval, pupal, and larva-adult development and yielded larvae with the lowest weight and survival and pupae with the lowest weight. Genotypes IAC 100 and DM 339 also negatively affected larval and pupal development and larval survival but at a lower level. Based on our results, the soybean lines PI 227687 and PI 227682 could be used as sources of genes for soybean breeding programs aiming to develop high yield, SAW-resistant cultivars. Moreover, further trials must be carried out under field conditions to validate if the commercial cultivars IAC 100 and DM 339, which expressed moderate levels of antibiosis-type resistance in the laboratory, are effective in suppressing SAW larvae populations. 相似文献
2.
M F Cabezas D E Nava L O Geissler M Melo M S Garcia R Krüger 《Neotropical Entomology》2013,42(6):588-594
Spodoptera cosmioides (Walker) (Lepidoptera: Noctuidae) is a polyphagous pest that threatens more than 24 species of crop plants including those used for biodiesel production such as Ricinus communis (castor bean), Jatropha curcas (Barbados nut), and Aleurites fordii (tung oil tree). The development and leaf consumption by S. cosmioides reared on leaves of these three species were studied under controlled laboratory conditions. The egg-to-adult development time of S. cosmioides was shortest when reared on castor bean leaves and longest when reared on tung oil tree leaves. Larvae reared on castor bean and Barbados nut leaves had seven instars, whereas those reared on tung oil tree leaves had eight. Females originating from larvae reared on castor bean and Barbados nut leaves showed greater fecundity than did females originating from larvae reared on tung oil tree leaves. Insects fed on castor bean leaves had shorter life spans than those fed on tung oil tree and Barbados nut leaves although the oviposition period did not differ significantly. The intrinsic and finite rates of increase were highest for females reared on castor bean leaves. Total leaf consumption was highest for larvae reared on tung oil tree leaves and lowest for those reared on Barbados nut leaves. We conclude that castor bean is a more appropriate host plant for the development of S. cosmioides than are Barbados nut and tung oil tree. 相似文献
3.
《Revista Brasileira de Entomologia》2019,63(3):238-244
This is the first report of Cotesia scotti (Valerio and Whitfield) comb. nov. in Brazil, attacking larvae of the black armyworm, Spodoptera cosmioides, and the southern armyworm, S. eridania. The moth larvae were found respectively, infesting a protected cropping of organic tomato in Hidrolândia, Goiás, Brazil, and a transgenic soybean crop in São José dos Pinhais, Paraná, Brazil. Biological, molecular and morphological characters were used to confirm the identity of the specimens. Parasitoid identification presented a challenge since the species has most diagnostic characters of the genus Cotesia Cameron, but few in the poorly defined genus Parapanteles Ashmead. Based on morphological and molecular evidence, we transfer Parapanteles scotti to the genus Cotesia. The new combination is discussed by comparison with morphologically similar species and available molecular data. 相似文献
4.
《Genomics》2020,112(6):3903-3914
The Southern armyworm Spodoptera eridania (Lepidoptera: Noctuidae) is native to the American tropics and a polyphagous pest of several crops. Here we characterized a novel alphabaculovirus isolated from S. eridania, isolate Spodoptera eridania nucleopolyhedrivurus CNPSo-165 (SperNPV-CNPSo-165). SperNPV-CNPSo-165 occlusion bodies were found to be polyhedral and to contain virions with multiple nucleocapsids. The virus was lethal to S. eridania and S. albula but not to S. frugiperda. The SperNPV-CNPSo-165 genome was 137.373 bp in size with a G + C content of 42.8%. We annotated 151 ORFs with 16 ORFs unique among baculoviruses. Phylogenetic inference indicated that this virus was closely related to the most recent common ancestor of other Spodoptera-isolated viruses. 相似文献
5.
The incubation period of Spodoptera exigua (Hübner) was not influenced by the host plant, whereas larval development time and pupal period were affected. Larval development time was longest on shallot and lady's finger, followed by cabbage and long bean. Larvae did not develop beyond the first instar when fed on chilli. The pupal period was longer on lady's finger than on cabbage, shallot and long bean. Overall, adult longevity was not influenced by the host plant but there was a difference between female and male longevity among the host plants. Survival of S. exigua was affected by the host plant at the larval stage. The number of larval instars varied between 5 and 8 within and between the studied host plants. Long bean was found to be the most suitable host plant and provide the best food quality for S. exigua compared to the other host plants, as it allowed faster development, fewer larval instars and a higher survival rate. 相似文献
6.
Naeem M. Eesa Hanaa El-Sherif Wael M. El-Sayed 《Invertebrate reproduction & development.》2017,61(1):27-33
The sensitivity of Spodoptera littoralis second and fourth instar larvae to the heavy metals cadmium (Cd) and lead (Pb) was investigated under laboratory conditions. Both Cd and Pb, even, at very low concentrations, had relatively high toxic effects on S. littoralis larvae. Cd was more toxic to S. littoralis larvae than Pb. In addition, the mean percentage feeding deterrence (FDI%) of Cd and Pb was concentration-dependent. The nutritional indices (consumption index, growth rate, efficiency of converted ingested and digested food, and approximate digestibility) of S. littoralis second and fourth instar larvae were more affected in Cd-treatments compared with those of Pb-treatments. Therefore, the presence of such heavy metals in the environment would exert an adverse impact on S. littoralis larvae and their development. 相似文献
7.
Martinelli S Barata RM Zucchi MI Silva-Filho Mde C Omoto C 《Journal of economic entomology》2006,99(2):519-526
The molecular variability among 10 populations of Spodoptera frugiperda (J.E. Smith), collected from maize, Zea mays L., or cotton Gossypium hirsutum L. crops located at distinctive geographical regions in Brazil, was assessed through random amplification of polymorphic DNA (RAPD)-polymerase chain reaction (PCR). In total, 208 RAPD markers were evaluated, and 98% of them were polymorphic. The mean genetic similarity was 0.6621 and 0.2499 by the Simple Matching and Jaccard matrices, respectively. In general, the unweighted pair-group method with arithmetic average dendrograms separated the populations into clusters related to the geographical origin of the samples. No branch of the dendrograms underpinning a molecular association of S. frugiperda has been identified to either of the two host plants. The molecular variance analysis showed that 18 and 82% of the genetic variation was distributed among and within the groups of populations, respectively. The principal coordinate analysis reinforced the pattern of population clustering found with the unweighted pair-group method with arithmetic average method. These results suggest the occurrence of considerable gene flow between S. frugiperda populations from maize and cotton fields located in the same region in Brazil. Therefore, for an effective management of this pest, there is an urgent need for a better understanding of the gene flow of S. frugiperda populations associated to different host plants along the distribution range of this pest over time in a specific cropping system. 相似文献
8.
A. L. WARD 《Australian Journal of Entomology》1997,36(1):75-79
Experiments were conducted using chemical irritants to develop a non-destructive sampling technique for pests of high quality turf and lawn. POW detergent at a concentration of 35 mL in 3.5 L of water applied to an area of 1,600 cm2 was the most effective for sampling Spodoptera mauritia and Herpetogramma licarsisalis and resulted in 84% of S. mauritia larvae surfacing within 4 min of application. No larvae surfaced after 4 min. Kendon pyrethrin SF applied at a concentration of 0.46 mL in 3.5 L of water resulted in similar numbers of larvae surfacing relative to 35 mL POW detergent. However, larvae took up to 20 min to surface. Formaldehyde and the pyrethroid, Mavrik® AF did not cause S. mauritia and H. licarsisalis larvae to surface. 相似文献
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11.
《Biological Control》2001,20(1):39-47
A Philippine Spodoptera litura nucleopolyhedrovirus (SltMNPV) was isolated by plaque purification from an uncloned NPV population in a formulated product that exhibits high insecticidal activity against the onion-attacking common cutworm, S. litura. Its estimated genome size was approximately 142 kb. Comparison with Autographa californica MNPV and Spodoptera exigua NPV by restriction fragmentation analysis showed that it was a distinct isolate. Light microscopy examination showed that SltMNPV-P7 infected insect cell lines derived from Spodoptera littoralis (CLS-79), Spodoptera frugiperda (Sf9), and S. litura (TUAT-SpLi221). It produced typical cytopathic effects in cell lines established from Bombyx mori and S. exigua and induced apoptotic-like cytopathology in Lymantria dispar-derived cell line; it exerted no observable cytopathic effect on Spilosoma imparilis cell line. Significant increase in budded virus (BV) production was observed in CLS-79, Sf9, and TUAT-SpLi221 cell lines, in which TUAT-SpLi221 cell line supported the highest BV titer. No significant BV production was observed in the remaining four cell lines. Similarly, viral DNA replication and polyhedrin production proceeded only in these three spodopteran cell lines. Results showed that SltMNPV-P7 is a unique isolate which can be propagated in vitro for further studies and thus has a potential for development into a more effective microbial insecticide. 相似文献
12.
Although various biological aspects of Spodoptera frugiperda (J.E. Smith) (Lepidoptera: Noctuidae) have been examined, adult movement and dispersal of this insect pest is not well understood. Release-recapture techniques by using marked insects is a useful approach for dispersal studies; however, the marking technique should not significantly affect insect biology or behavior. Therefore, the effect of different concentrations of oil-soluble dyes (Solvent Blue 35 [C.I. 61554], Sudan Red 7B [C.I. 26050], Sudan Black B [26150], Sudan Orange G [C.I. 11920], and Sudan I 103624 [C.I. 12055]) on development, mortality, and fecundity of S. frugiperda was evaluated. Dyes were added to artificial diet used to feed larvae. Larval and pupal development and mortality, adult longevity, and female fecundity were evaluated. High concentrations (400 and 600 ppm) of all dyes led to longer larval and pupal stages. Adult life span and number of eggs were not affected by the dyes. Sudan Red 7B marked both adults and eggs very well. Solvent Blue 35 marked both adults and eggs, but the blue-marked eggs could not be distinguished from some bluish eggs laid by nonlabeled females. Adults and eggs were not adequately marked by the Sudan Black B, Sudan Orange G, and Sudan I 103624 (a yellow dye). 相似文献
13.
《Insect Biochemistry》1987,17(6):877-881
Female pheromone gland extracts of Spodoptera littoralis were analyzed for pheromone precursors. Large amounts of fatty methyl esters were found and a positive relationship between the methyl esters and the pheromonal components was observed. The esters were identified on the basis of capillary gas chromatography, coupled gas chromatography with mass spectroscopy and dimethyl disulfide derivatization, and subsequent gas chromatography with mass spectrometry. The characteristic fatty esters of S. littoralis are methyl (Z)-9-tetradecenoate, (E)- and (Z)-11-tetradecenoate, (Z,E)-9,12-tetradecadienoate, (Z,E)-9,11-tetradecadienoate, and (Z)-11-hexadecenoate. The biosynthesis of the monosaturated acids involves probably the common E11 and Z11 desaturases and chain shortening. For the biosynthesis of the novel diene acids, we propose a second, specific desaturation of (Z)-9-tetradecenoate by an E11 desaturase to produce (Z,E)-9,11-tetradecadienoate or by an E12 desaturase to produce (Z,E)-9,12-tetradecadienoate. 相似文献
14.
Dr. J. Godula 《Cell and tissue research》1985,242(3):681-683
Summary The surface morphology of primary spermatocytes from testicular cysts of the last instar larvae of Spodoptera littoralis was examined by scanning electron microscopy (SEM). At this stage, each primary spermatocyte possesses four developing flagella, directed towards the lumen of the cyst. The identical length of flagella in all primary spermatocytes from a single cyst indicates that the initiation and rate of flagellar growth are synchronized. Some asynchrony can, however, be observed in the translocation of flagella to secondary spermatocytes during the first meiotic division. 相似文献
15.
《Journal of Asia》2000,3(2):55-58
In vitro metabolism study was conducted to observe the role of enzymes and its kind responsible for tolerance in metabolism of flupyrazofos to Diamond backmoth (DBM), Plutella xylostella as a susceptible insect and Beet armyworm (BAW), Spodoptera exigua as a tolerance insect. The results indicated that there were some differences between the DBM (LD50, 0.54 ppm) and BAW (LD50, >2,000 ppm). In the microsomal fractions, the 4th instar BAW larvae metabolized almost the same amount (about 38%) of flupyrazofos with or without NADPH, but it increased the amount of flupyrazol in the 3rd instar larvae of DBM. Flupyrazofos-oxon was slightly increased with the addition of NADPH in both species. With the addition of piperonyl butoxide (PB) or Iprobenfos (IBP), the amount of flupyrazol was decreased to one third of flupyrazol produced by NADPH in BAW and one fourth of flupyrazol produced by NADPH in DBM. Some effects of inhibitors, PB and IBP, and co-factor, NADPH, were showed when flupyrazofos was metabolized with or without them. This means that esterase and oxidase are responsible for the tolerance mechanism of flupyrazofos in DBM and BAW. 相似文献
16.
Sergio Pérez-Guerrero Asnake Gelan-Begna Rafael Tamajón Enrique Vargas-Osuna 《Biocontrol Science and Technology》2013,23(3):335-347
The relative feeding rates and preferences of a hunting-spider assemblage inhabiting southern Spanish cotton fields for two major cotton pests, Helicoverpa armigera (Hübner) and Spodoptera littoralis (Boisdubal) (Lepidoptera: Noctuidae), were analyzed under laboratory conditions. First, a no-choice feeding test was used to determine relative feeding rates for hunting-spider families and species, offering a fixed number of 10 neonate larvae of H. armigera or S. littoralis and observing predation after 2 h, 4 h, 8 h, and 24 h. In a second test, Drosophila melanogaster, a very palatable alternative prey, was used to determine the degree of preference for cotton pest larvae. The mean number of first-instar lepidoptera larvae consumed by hunting spiders after 24 h was 8.57±0.25. As expected, spiders showed no preference for either of the two cotton pest species H. armigera and S. littoralis over the other. Results also showed that cursorial spiders of the families Miturigidae (represented here by Cheiracanthium pelasgicum) and Philodromidae consumed significantly higher percentages of larvae than crab spiders belonging to the Thomisidae family after 2 h and 24 h, respectively. In the prey choice test, Cheiracantium pelasgicum displayed a strong preference for cotton pest larvae while Thomisidae and Oxyopidae showed no significant preference. In addition, as the attack sequence progressed, Ch. pelasgicum showed a clear tendency towards the alternation of prey while Thomisidae, and more irregularly Oxyopidae, maintained their preference for D. melanogaster. These findings confirmed both the considerable potential value of some cursorial spiders (e.g. Ch. pelasgicum) in the biological control of lepidopteran cotton pests and the relatively low impact of other hunting spiders, e.g. Thomisidae, on pests of this kind. 相似文献
17.
杀菌剂丙环唑对斜纹夜蛾的毒性 总被引:2,自引:2,他引:2
在研究比较了11种农药对斜纹夜蛾Spodoptera litura细胞(简称SL细胞)的毒杀活性基础上,选择毒杀活性最高的杀菌剂丙环唑,对其毒理学机理进行进一步研究。结果表明,丙环唑的细胞毒力最高,在100 μg/mL浓度下处理后48 h,SL细胞的死亡率为98.08%。处理后36 h,丙环唑对SL细胞的LC50值为20.31 μg/mL。丙环唑能明显降低SL细胞的蛋白质含量。以0.5 μg/头的丙环唑注射斜纹夜蛾4龄幼虫,处理后72 h,试虫血淋巴总含量及血细胞数分别下降了26.80%和25.26%;在1.0 μg/头的剂量下,则分别下降了37.67%和36.32%。以0.5 μg/头和1.0 μg/头的丙环唑注射处理后,斜纹夜蛾幼虫体重显著降低。此外,丙环唑能降低斜纹夜蛾幼虫血淋巴含糖量及血淋巴蛋白质含量。在注射处理后96 h和120 h,丙环唑对斜纹夜蛾4龄幼虫的LD50值分别为0.59 μg/头和0.45 μg/头。丙环唑对SL细胞和斜纹夜蛾幼虫均具有较好的毒杀活性,显示出丙环唑类似物控制害虫的可能性。 相似文献
18.
Cross-resistance and genetics of resistance to indoxacarb in Spodoptera litura (Lepidoptera: Noctuidae) 总被引:2,自引:0,他引:2
Bioassays (at generation G1) with a newly collected field population of Spodoptera litura (F.) (Lepidoptera: Noctuidae) from Multan, Pakistan, showed resistance ratios of 15, 23, 37, and 16 for indoxacarb, spinosad, abamectin, and emamectin, respectively, compared with a laboratory susceptible population, Lab-PK. At G1, the field population was selected with indoxacarb by using single pair crosses. For Indoxa-SEL, bioassay at G4 found that selection increased resistance ratio to 95 for indoxacarb compared with Lab-PK. Selection with indoxacarb significantly increased resistance to spinosad and emamectin; however, resistance to abamectin was observed to drop. A significant reduction in the resistance to indoxacarb was observed in Indoxa-SEL at G9, indicating unstable resistance to indoxacarb; however, it was stable for fipronil. Synergism tests with microsomal oxidase and esterase-specific inhibitors suggested that the indoxacarb resistance was associated with microsomal oxidase. Reciprocal genetic crosses between Indoxa-SEL and Lab-PK populations indicated that resistance was autosomal and incompletely dominant. Tests of monogenic inheritance suggested that resistance to indoxacarb was controlled by more than one locus. 相似文献
19.
Population dynamics of Spodoptera litura (Lepidoptera: Noctuidae) on Bt cotton in the Yangtze River Valley of China 总被引:3,自引:0,他引:3
Genetically modified cotton that produces a crystalline protein from Bacillus thuringiensis subsp. kurstaki (Berliner) (Bt) has been widely deployed to manage lepidopteran insect pests in cotton growing areas worldwide. However, susceptibility of different insect species to Bt protein varies, which may affect lepidopteran pest populations in the field. Studies on effects of two transgenic cotton lines (BG1560 and GK19) carrying a Cry1A gene on common cutworm Spodoptera litura F. (Lepidoptera: Noctuidae), were conducted during 2002-2005 in the cotton planting region of the Yangtze River valley of China. Results showed that common cutworm larvae had low susceptibility to Bt cotton. There was no significant difference in larval population densities in conventional and Bt cotton fields. However, the larval populations of the insect on conventional plants treated with chemical insecticides for control of target pest of Bt cotton were significantly lower than that in Bt cotton fields. These results indicated that the common cutworm was the potential to become a major and alarming pest in Bt cotton fields, and therefore efforts to develop an effective alternative management strategy are needed. 相似文献
20.
J. Mark Scriber 《Oecologia》1981,51(2):175-180
Summary This study illustrates the diversity of feeding responses of individually polyphagous southern armyworms, Spodoptera eridania, to plants with differing allelochemics. In spite of the near optimal leaf water and nitrogen contents of the young foliage, it is apparent that vastly different larval growth performance results from dill, lima bean, and cabbage. Cabbage is the poorest food (as measured by larval growth rates and metabolic costs of processing the plant biomass). Unlike the case with certain other plant species or cultivars that are costly to process, with cabbage, S. eridania does not compensate for low efficiencies (E.C.D.'s) with increased consumption rates (R.C.R.'s). Biochemical or physiological reasons for this inability are unknown.A sequence of foods (changed each 18–24 h) apparently did not add sufficient stress upon the MFO system to be detected in the respiratory expenditures of S. eridania larvae, in spite of the fact that dill is known to contain insecticidal and synergistic chemicals (Lichtenstein et al. 1974). The larval growth performances and metabolic expenditures in these sequences were intermediate between the best food (dill) and the worse (cabbage). Significant differences were observed however between the sequential switching sequences, perhaps indicating that particular periods during the instar are especially more sensitive to certain allelochemics. Actual respiratory costs of the lima bean-cabbage-dill (i.e. B-C-D) sequence were 40–50% higher than observed for the other two sequences and more than 50% higher than the theoretical metabolic costs based on the proportions actually eaten and known costs associated with each food.This study and a related one (Scriber 1981a) illustrate how consumption rates, feeding efficiences, and larval growth of Spodoptera eridania are not species, population, or even individual characteristics, (cf. Fox and Morrow 1981), but instead depend largely upon variations in plant allelochemics and plant nutritional quality (Wolfson 1978; Scriber, 1981 b; Scriber and Slansky 1981). More significantly they illustrate that the food consumed in earlier instars (Scriber 1981 a) as well as the food consumed earlier in an instar can be a major influence upon the observed armyworm growth performances under a given set of environmental conditions.A publication of the College of Agriculture and Life Sciences, University of Wisconsin, Madison 53706Mention of a product or brand name does not reflect an endorsement of that item by the University of Wisconsin 相似文献