共查询到20条相似文献,搜索用时 31 毫秒
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A DNA polymorphism specific to Candida albicans strains exceptionally successful as human pathogens 总被引:1,自引:0,他引:1
Giblin L Edelmann A Zhang N von Maltzahn NB Cleland SB Sullivan PA Schmid J 《Gene》2001,265(1-2):157-164
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Soullier S Jay P Poulat F Vanacker JM Berta P Laudet V 《Journal of molecular evolution》1999,48(5):517-527
From a database containing the published HMG protein sequences, we constructed an alignment of the HMG box functional domain
based on sequence identity. Due to the large number of sequences (more than 250) and the short size of this domain, several
data sets were used. This analysis reveals that the HMG box superfamily can be separated into two clearly defined subfamilies:
(i) the SOX/MATA/TCF family, which clusters proteins able to bind to specific DNA sequences; and (ii) the HMG/UBF family,
which clusters members which bind non specifically to DNA. The appearance and diversification of these subfamilies largely
predate the split between the yeast and the metazoan lineages. Particular emphasis was placed on the analysis of the SOX subfamily.
For the first time our analysis clearly identified the SOX subfamily as structured in six groups of genes named SOX5/6, SRY,
SOX2/3, SOX14, SOX4/22, and SOX9/18. The validity of these gene clusters is confirmed by their functional characteristics
and their sequences outside the HMG box. In sharp contrast, there are only a few robust branching patterns inside the UBF/HMG
family, probably because of the much more ancient diversification of this family than the diversification of the SOX family.
The only consistent groups that can be detected by our analysis are HMG box 1, vertebrate HMG box 2, insect SSRP, and plant
HMG. The various UBF boxes cannot be clustered together and their diversification appears to be extremely ancient, probably
before the appearance of metazoans.
Received: 20 July 1998 / Accepted: 19 October 1998 相似文献
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Mutations in the transcription factor gene SOX18 underlie recessive and dominant forms of hypotrichosis-lymphedema-telangiectasia 总被引:1,自引:0,他引:1
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Irrthum A Devriendt K Chitayat D Matthijs G Glade C Steijlen PM Fryns JP Van Steensel MA Vikkula M 《American journal of human genetics》2003,72(6):1470-1478
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扬子鳄4个Sox基因保守区的克隆及序列分析 总被引:4,自引:0,他引:4
参考人SRY基因HMG-box的保守区序列,设计一对简并引物,用PCR扩增了扬子鳄Sox基因的HMG-box,并对PCR产物进行了亚克隆和测序。结果在雌雄个体中均筛选到4个不同的Sox基因,无性别差异。其序列与人相应的SOX基因保守区编码序列的相似性分别为91%、96%、100%、96%,分别命名为AS-Sox1,ASSox2,ASSox11,ASSox22。与其他动物相关的Sox/SOX基因的聚类分析结果表明,扬子鳄Sox基因编码的氨基酸序列与进化位置各异的其他动物的Sox/SOX基因编码的氨基酸序列存在高度的同源性,显示出Sox基因在系统进化上的高度保守性。 相似文献
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Involvement of SOX proteins in lens-specific activation of crystallin genes. 总被引:16,自引:0,他引:16
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Y Kamachi S Sockanathan Q Liu M Breitman R Lovell-Badge H Kondoh 《The EMBO journal》1995,14(14):3510-3519
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DNA binding and bending properties of the post-meiotically expressed Sry-related protein Sox-5. 总被引:8,自引:2,他引:6
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F Connor P D Cary C M Read N S Preston P C Driscoll P Denny C Crane-Robinson A Ashworth 《Nucleic acids research》1994,22(16):3339-3346
Sox-5 is one of a family of genes which show homology to the HMG box region of the testis determining gene SRY. We have used indirect immunofluorescence to show that Sox-5 protein is localized to the nucleus of post-meiotic round spermatids in the mouse testis. In vitro footprinting and gel retardation assays demonstrate that Sox-5 binds specifically to the sequence AACAAT with moderately high affinity (Kd of approximately 10(-9) M). Moreover, interaction of Sox-5 with its target DNA induces a significant bend in the DNA, characteristic of HMG box proteins. Circular dichroism spectroscopy of the Sox-5 HMG box and its specific complex with DNA shows an alteration in the DNA spectrum, perhaps as a consequence of DNA bending, but none in the protein spectrum on complex formation. The dependence of the change in the CD spectrum with protein to DNA ratio demonstrates the formation of a 1:1 complex. Analysis of the structure of the Sox-5 HMG box by 2D NMR suggests that both the location of helical secondary structure as well as the tertiary structure is similar to that of HMG1 box 2. 相似文献