共查询到20条相似文献,搜索用时 32 毫秒
1.
J. D. Sherman A. L. Fenwick D. M. Namuth N. L. V. Lapitan 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1995,91(4):681-690
Several gene linkage maps have been produced for cultivated barley. We have produced a new linkage map for barley, based on a cross between Hordeum vulgare subsp. spontaneum and Hordeum vulgare subsp. vulgare (Hvs x Hvv), having a higher level of polymorphism than most of the previous barley crosses used for RFLP mapping. Of 133 markers mapped in the Hvs x Hvv F2 population, 69 were previously mapped on other barley maps, and 26 were mapped in rice, maize, or wheat. Two known gene clones were mapped as well as two morphological markers. The distributions of previously mapped markers were compared with their respective barley maps to align the different maps into one consensus map. The distributions of common markers among barley, wheat, rice and maize were also compared, indicating colinear linkage groups among these species.To be considered dual first authorsPublished with the approval of the Director of the Colorado State University/Agricultural Experiment Station. 相似文献
2.
D. Menancio-Hautea C. A. Fatokun L. Kumar D. Danesh N. D. Young 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1993,86(7):797-810
Genome relationships between mungbean (Vigna tradiata) and cowpea (V. Unguiculata) based on the linkage arrangement of random genomic restriction fragment length polymorphism (RFLP) markers have been investigated. A common set of probes derived from cowpea, common bean (Phaseolus vulgaris), mungbean, and soybean (Glycine max) PstI genomic libraries were used to construct the genetic linkage maps. In both species, a single F2 population from a cross between an improved cultivar and a putative wild progenitor species was used to follow the segregation of the RFLP markers. Approximately 90% of the probes hybridized to both mungbean and cowpea DNA, indicating a high degree of similarity in the nucleotide sequences among these species. A higher level of polymorphism was detected in the mungbean population (75.7%) than in the cowpea population (41.2%). Loci exhibiting duplications, null phenotypes, and distorted segregation ratios were detected in both populations. Random genomic DNA RFLP loci account for about 89% of the currently mapped markers with a few cDNA and RAPD markers added. The current mungbean map is comprised of 171 loci/loci clusters distributed in 14 linkage groups spanning a total of 1570cM. On the other hand, 97 markers covered 684 cM and defined 10 linkage groups in the current cowpea map. The mungbean and cowpea genomes were compared on the basis of the copy number and linkage arrangement of 53 markers mapped in common between the two species. Results indicate that nucleotide sequences are conserved, but variation in copy number were detected and several rearrangements in linkage orders appeared to have occurred since the divergence of the two species. Entire linkage groups were not conserved, but several large linkage blocks were maintained in both genomes. 相似文献
3.
Ayliffe MA Mitchell HJ Deuschle K Pryor AJ 《Molecular genetics and genomics : MGG》2005,274(5):494-505
Proline accumulation and catabolism play significant roles in adaptation to a variety of plant stresses including osmotic
stress, drought, temperature, freezing, UV irradiation, heavy metals and pathogen infection. In this study, the gene Δ1 -pyrroline-5-carboxylate dehydrogenase (P5CDH), which catalyzes the second step in the conversion of proline to glutamate, is characterized in a number of cereal species.
P5CDH genes from hexaploid wheat, Triticum turgidum (durum wheat), Aegilops tauschii, Triticum monococcum, barley, maize and rice were shown to be conserved in terms of gene structure and sequence, present as a single copy per
haploid, non-polyploid genome and located in evolutionarily conserved linkage groups. A wheat cDNA sequence was shown by yeast
complementation to encode a functional P5CDH activity. A divergently-transcribed rab7 gene was identified immediately 5′ of P5CDH in all grasses examined, except rice. The rab7/P5CDH intergenic region in these species, which presumably encompasses 5′ regulatory elements of both genes, showed a distinct
pattern of sequence evolution with sequences in juxtaposition to each ORF conserved between barley, wheat, A. tauschii and T. monococcum. More distal 5′ sequence in this intergenic region showed a higher rate of divergence, with no homology observed between
these regions in the wheat and barley genomes. Maize and rice showed no similarity in regions 5′ of P5CDH when compared with wheat, barley, and each other, apart from a 22 bp region of conserved non-coding sequence (CNS) that is
similar to a proline response element identified in the promoter of the Arabidopsis proline dehydrogenase gene. A palindromic
motif similar to this cereal CNS was also identified 5′ of the Arabidopsis AtP5CDH gene showing conservation of this sequence in monocot and dicot lineages. 相似文献
4.
A. Kaga M. Ohnishi T. Ishii O. Kamijima 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1996,93(5-6):658-663
A genetic linkage map of azuki bean (Vigna angularis) was constructed with molecular and morphological markers using an F2 population of an interspecific cross between azuki bean and its wild relative, V. nakashimae. In total, 132 markers (108 RAPD, 19 RFLP and five morphological markers) were mapped in 14 linkage groups covering 1250 cM; ten remained unlinked. The clusters of markers showing distorted segregation were found in linkage groups 2, 8 and 12. By comparing the azuki linkage map with those of mungbean and cowpea, using 20 RFLP common markers, some sets of the markers were found to belong to the same linkage groups of the respective maps, indicating that these linkage blocks are conserved among the three Vigna species. This map provides a tool for markerassisted selection and for studies of genome organization in Vigna species. 相似文献
5.
Genetic Map of Diploid Wheat, Triticum Monococcum L., and Its Comparison with Maps of Hordeum Vulgare L 总被引:6,自引:0,他引:6
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J. Dubcovsky M. C. Luo G. Y. Zhong R. Bransteitter A. Desai A. Kilian A. Kleinhofs J. Dvorak 《Genetics》1996,143(2):983-999
A genetic map of diploid wheat, Triticum monococcum L., involving 335 markers, including RFLP DNA markers, isozymes, seed storage proteins, rRNA, and morphological loci, is reported. T. monococcum and barley linkage groups are remarkably conserved. They differ by a reciprocal translocation involving the long arms of chromosomes 4 and 5, and paracentric inversions in the long arm of chromosomes 1 and 4; the latter is in a segment of chromosome arm 4L translocated to 5L in T. monococcum. The order of the markers in the inverted segments in the T. monococcum genome is the same as in the B and D genomes of T. aestivum L. The T. monococcum map differs from the barley maps in the distribution of recombination within chromosomes. The major 5S rRNA loci were mapped on the short arms of T. monococcum chromosomes 1 and 5 and the long arms of barley chromosomes 2 and 3. Since these chromosome arms are colinear, the major 5S rRNA loci must be subjected to positional changes in the evolving Triticeae genome that do not perturb chromosome colinearity. The positional changes of the major 5S rRNA loci in Triticeae genomes are analogous to those of the 18S-5.8S-26S rRNA loci. 相似文献
6.
Development of simple sequence repeat DNA markers and their integration into a barley linkage map 总被引:29,自引:0,他引:29
Z. -W. Liu R. M. Biyashev M. A. Saghai Maroof 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1996,93(5-6):869-876
Simple sequence repeats (SSRs), or microsatellites, are a new class of PCR-based DNA markers for genetic mapping. The objectives of the present study were to develop SSR markers for barley and to integrate them into an existing barley linkage map. DNA sequences containing SSRs were isolated from a barley genomic library and from public databases. It is estimated that the barley genome contains one (GA)n repeat every 330 kb and one (CA)n repeat every 620 kb. A total of 45 SSRs were identified and mapped to seven barley chromosomes using doubled-haploid lines and/or wheat-barley addition-line assays. Segregation analysis for 39 of these SSRs identified 40 loci. These 40 markers were placed on a barley linkage map with respect to 160 restriction fragment length polymorphism (RFLP) and other markers. The results of this study demonstrate the value of SSRs as markers in genetic studies and breeding research in barley. 相似文献
7.
Comparative RFLP mapping of meadow and tall fescue 总被引:7,自引:2,他引:5
C. Chen D. A. Sleper G. S Johal 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1998,97(1-2):255-260
Molecular markers based on restriction fragment length polymorphism (RFLP) were used to construct a genetic linkage map in
diploid meadow fescue, Festuca pratensis Huds. (2n=2x=14, genomic designation PP), and to compare its genomic relationship with a related species, hexaploid tall fescue (Festuca arundinacea Schreb.; 2n=6x=42, PPG1G1G2G2). Using a collection of 66 tall-fescue (heterologous) markers, an RFLP linkage map was constructed in F. pratensis. This map, which has a total length of 280.1 cM, includes seven linkage groups. A comparison of 33 markers that were mapped
in both F. pratensis and F. arundinacea detected highly conserved linkage groups between these two species. Our data are consistent with the proposal that one of
the genomes of F. arundinacea was derived from F. pratensis. However, since significant changes in marker sequences, map distances, and homoeologous linkage groups were also detected
between the two species, it appears that the P genome diverged substantially during evolution from the diploid to the hexaploid
Festuca.
Received: 23 May 1997 / Accepted: 15 January 1998 相似文献
8.
M. J. Truco C. F. Quiros 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1994,89(5):590-598
We constructed a genetic map on Brassica nigra based on a segregating population of 83 F2 individuals. Three different types of molecular markers were used to build the map including isozymes, restriction fragment length polymorphisms (RFLP), and random amplified polymorphic DNA (RAPD). The final map contained 124 markers distributed in 11 linkage groups. The map covered a total distance of 677 cM with the markers distributed within a mean distance of 5.5cM. Of the sequences found in the B. nigra map, 40% were duplicated and organized into three different types of arrangements. They were either scattered throughout the genome, organized in tandem, or organized in blocks of duplicated loci conserved in more than 1 linkage group. 相似文献
9.
Cytologically integrated physical restriction fragment length polymorphism maps for the barley genome based on translocation breakpoints 总被引:33,自引:0,他引:33
We have developed a new technique for the physical mapping of barley chromosomes using microdissected translocation chromosomes for PCR with sequence-tagged site primers derived from >300 genetically mapped RFLP probes. The positions of 240 translocation breakpoints were integrated as physical landmarks into linkage maps of the seven barley chromosomes. This strategy proved to be highly efficient in relating physical to genetic distances. A very heterogeneous distribution of recombination rates was found along individual chromosomes. Recombination is mainly confined to a few relatively small areas spaced by large segments in which recombination is severely suppressed. The regions of highest recombination frequency (=1 Mb/cM) correspond to only 4.9% of the total barley genome and harbor 47.3% of the 429 markers of the studied RFLP map. The results for barley correspond well with those obtained by deletion mapping in wheat. This indicates that chromosomal regions characterized by similar recombination frequencies and marker densities are highly conserved between the genomes of barley and wheat. The findings for barley support the conclusions drawn from deletion mapping in wheat that for all plant genomes, notwithstanding their size, the marker-rich regions are all of similar gene density and recombination activity and, therefore, should be equally accessible to map-based cloning. 相似文献
10.
W. Kennard R. Phillips R. Porter A. Grombacher R. L. Phillips 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1999,99(5):793-799
We present the first genetic map of wild rice (Zizania palustris L., 2n=2x=30), a native aquatic grain of northern North America. This map is composed principally of previously mapped RFLP (restriction
fragment length polymorphism) genetic markers from rice (Oryza sativa 2n=2x=24). The map is important as a foundation for genetic and crop improvement studies as well as a reference for genome organization
comparisons among species of Gramineae. A comparative mapping approach with rice is especially useful because wild rice is
grouped in the same subfamily, Oryzoideae, and no other mapping comparison has yet been made within the subfamily. As rice
is the reference point for mapping and gene cloning in cereals, establishing a consensus map within the subfamily identifies
conserved and unique regions. The genomes of wild rice and rice differ in total DNA content (wild rice has twice that of rice)
and the number of chromosome pairs (wild rice=15 versus rice=12). The wild rice linkage map reported herein consists of 121
RFLP markers on 16 linkage groups spanning 1805 cM. Two linkage groups consist of only two markers. Colinear markers were
found representing all rice linkage groups except #12. The majority of rice loci mapped to colinearly arranged arrays in wild
rice (92 of 118). Features of the map include duplication of portions of three rice linkage groups and three possible translocations.
The map gives basic information on the composition of the wild rice genome and provides tools to assist in the domestication
of this important food source.
Received: 25 August 1998 / Accepted: 20 February 1999 相似文献
11.
Singh K Ghai M Garg M Chhuneja P Kaur P Schnurbusch T Keller B Dhaliwal HS 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2007,115(3):301-312
Diploid A genome species of wheat harbour immense variability for biotic stresses and productivity traits, and these could
be transferred efficiently to hexaploid wheat through marker assisted selection, provided the target genes are tagged at diploid
level first. Here we report an integrated molecular linkage map of A genome diploid wheat based on 93 recombinant inbred lines
(RILs) derived from Triticum boeoticum × Triticum monococcum inter sub-specific cross. The parental lines were analysed with 306 simple sequence repeat (SSR) and 194 RFLP markers, including
66 bin mapped ESTs. Out of 306 SSRs tested for polymorphism, 74 (24.2%) did not show amplification (null) in both the parents.
Overall, 171 (73.7%) of the 232 remaining SSR and 98 (50.5%) of the 194 RFLP markers were polymorphic. Both A and D genome
specific SSR markers showed similar transferability to A genome of diploid wheat species. The 176 polymorphic markers, that
were assayed on a set of 93 RILs, yielded 188 polymorphic loci and 177 of these as well as two additional morphological traits
mapped on seven linkage groups with a total map length of 1,262 cM, which is longer than most of the available A genome linkage
maps in diploid and hexaploid wheat. About 58 loci showed distorted segregation with majority of these mapping on chromosome
2Am. With a few exceptions, the position and order of the markers was similar to the ones in other maps of the wheat A genome.
Chromosome 1Am of T. monococcum and T. boeoticum showed a small paracentric inversion relative to the A genome of hexaploid wheat. The described linkage map could be useful
for gene tagging, marker assisted gene introgression from diploid into hexaploid wheat as well as for map based cloning of
genes from diploid A genome species and orthologous genes from hexaploid wheat. 相似文献
12.
Ulloa M Meredith WR Shappley ZW Kahler AL 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2002,104(2-3):200-208
An RFLP genetic linkage joinmap was constructed from four different mapping populations of cotton (Gossypium hirsutum L.). Genetic maps from two of the four populations have been previously reported. The third genetic map was constructed from
199 bulk-sampled plots of an F2.3 (HQ95–6×’MD51ne’) population. The map comprises 83 loci mapped to 24 linkage groups with an average distance between markers
of 10.0 centiMorgan (cM), covering 830.1 cM or approximately 18% of the genome. The fourth genetic map was developed from
155 bulk-sampled plots of an F2.3 (119– 5 sub-okra×’MD51ne’) population. This map comprises 56 loci mapped to 16 linkage groups with an average distance between
markers of 9.3 cM, covering 520.4 cM or approximately 11% of the cotton genome. A core of 104 cDNA probes was shared between
populations, yielding 111 RFLP loci. The constructed genetic linkage joinmap from the above four populations comprises 284
loci mapped to 47 linkage groups with the average distance between markers of 5.3 cM, covering 1,502.6 cM or approximately
31% of the total recombinational length of the cotton genome. The linkage groups contained from 2 to 54 loci each and ranged
in distance from 1.0 to 142.6 cM. The joinmap provided further knowledge of competitive chromosome arrangement, parental relationships,
gene order, and increased the potential to map genes for the improvement of the cotton crop. This is the first genetic linkage
joinmap assembled in G. hirsutum with a core of RFLP markers assayed on different genetic backgrounds of cotton populations (Acala, Delta, and Texas plain).
Research is ongoing for the identification of quantitative trait loci for agronomic, physiological and fiber quality traits
on these maps, and the identification of RFLP loci lineage for G. hirsutum from its diploid progenitors (the A and D genomes).
Received: 23 February 2001 / Accepted: 8 June 2001 相似文献
13.
Comparative genetic maps of foxtail millet (Setaria italica) and rice (Oryza sativa) 总被引:4,自引:0,他引:4
K. M. Devos Z. M. Wang J. Beales T. Sasaki M. D. Gale 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1998,96(1):63-68
A foxtail millet-rice comparative genetic map was constructed using mapped rice RFLP markers and wheat genomic and cDNA clones
with known map position in rice. About 74% and 37% of the cDNA and genomic clones, respectively, were transferable to foxtail
millet, confirming that conservation at the DNA level is greatest in genic regions. A high degree of conserved colinearity
was observed between the two genomes. Five entire foxtail millet chromosomes appear to be colinear with five entire rice chromosomes.
The remaining four foxtail millet linkage groups each show colinearity with segments of two rice chromosomes. The rearrangements
of rice chromosomes 3 and 10 to form foxtail millet chromosome IX, and 7 and 9 to form chromosome II are very similar to those
required to form maize chromosomes 1 and 7 and sorghum linkage groups C and B, indicating Setaria’s clear taxonomic position within the subfamily of the Panicoideae.
Received: 18 December 1996 / Accepted: 4 August 1997 相似文献
14.
Boyko Elena Kalendar Ruslan Korzun Victor Fellers John Korol Abraham Schulman Alan H. Gill Bikram S. 《Plant molecular biology》2002,48(5-6):767-789
Aegilops tauschii (Coss.) Schmal. (2n=2x=14, DD) (syn. A. squarrosa L.; Triticum tauschii) is well known as the D-genome donor of bread wheat (T. aestivum, 2n=6x=42, AABBDD). Because of conserved synteny, a high-density map of the A. tauschii genome will be useful for breeding and genetics within the tribe Triticeae which besides bread wheat also includes barley and rye. We have placed 249 new loci onto a high-density integrated cytological and genetic map of A. tauschii for a total of 732 loci making it one of the most extensive maps produced to date for the Triticeae species. Of the mapped loci, 160 are defense-related genes. The retrotransposon marker system recently developed for cultivated barley (Hordeum vulgare L.) was successfully applied to A. tauschii with the placement of 80 retrotransposon loci onto the map. A total of 50 microsatellite and ISSR loci were also added. Most of the retrotransposon loci, resistance (R), and defense-response (DR) genes are organized into clusters: retrotransposon clusters in the pericentromeric regions, R and DR gene clusters in distal/telomeric regions. Markers are non-randomly distributed with low density in the pericentromeric regions and marker clusters in the distal regions. A significant correlation between the physical density of markers (number of markers mapped to the chromosome segment/physical length of the same segment in m) and recombination rate (genetic length of a chromosome segment/physical length of the same segment in m) was demonstrated. Discrete regions of negative or positive interference (an excess or deficiency of crossovers in adjacent intervals relative to the expected rates on the assumption of no interference) was observed in most of the chromosomes. Surprisingly, pericentromeric regions showed negative interference. Islands with negative, positive and/or no interference were present in interstitial and distal regions. Most of the positive interference was restricted to the long arms. The model of chromosome structure and function in cereals with large genomes that emerges from these studies is discussed. 相似文献
15.
S. Groh A. Zacharias S. F. Kianian G. A. Penner J. Chong H. W. Rines R. L. Phillips 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2001,102(6-7):876-884
Amplified fragment length polymorphisms (AFLPs) can be used to quickly develop linkage maps in plant species and are especially
useful for crops with large genomes like oat (Avena sativa L., 2n=6x=42). High reproducibility and consistency are crucial if AFLP linkage maps are employed for comparative mapping.
We mapped AFLP markers in combination with restriction fragment length polymorphism (RFLP) markers in two recombinant inbred
populations of hexaploid oat in two laboratories to test the consistency of AFLP markers in a polyploid crop. Eight primer
combinations produced 102 and 121 scoreable AFLP markers in the respective populations. In a population from the cross Kanota×Ogle,
AFLP markers were placed onto a RFLP reference map consisting of 32 linkage groups. Nineteen linkage groups from another population
from the cross Kanota×Marion were assigned to the reference map using AFLP and RFLP markers homologous to those used in the
Kanota× Ogle cross. Reproducibility of AFLP assays was high in both laboratories and between laboratories. The AFLP markers
were well-distributed across the genome in both populations. Many AFLP markers tended to extend the distance between adjacent
RFLP markers in linkage analysis. Of the 27 polymorphic AFLPs common in both populations, 20 mapped to homologous linkage
groups, 4 were unlinked in at least one population, and 3 mapped to different linkage groups in the two crosses. We believe
that 1 of the 3 markers that mapped to a different linkage group in the two populations mapped to homoeologous linkage groups.
The linkage map of hexaploid oat is not yet complete, and genomic rearrangements such as translocations exist among cultivars
and are likely to account for the remaining two non-syntenous mapping results. AFLPs provide not only a fast and powerful
tool for mapping but could be useful in characterizing genomic structural variations among germplasms in hexaploid oat.
Received: 17 December 1999 / Accepted: 28 July 2000 相似文献
16.
Comparison of the genetic maps of Brassica napus and Brassica oleracea 总被引:14,自引:0,他引:14
W. Y. Cheung G. Champagne N. Hubert B. S. Landry 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1997,94(5):569-582
The genus Brassica consists of several hundreds of diploid and amphidiploid species. Most of the diploid species have eight, nine or ten pairs
of chromosomes, known respectively as the B, C, and A genomes. Genetic maps were constructed for both B. napus and B. oleracea using mostly RFLP and RAPD markers. For the B. napus linkage map, 274 RFLPs, 66 RAPDs, and two STS loci were arranged in 19 major linkage groups and ten smaller unassigned segments,
covering a genetic distance of 2125 cM. A genetic map of B. oleracea was constructed using the same set of RFLP probes and RAPD primers. The B. oleracea map consisted of 270 RFLPs, 31 RAPDs, one STS, three SCARs, one phenotypic and four isozyme marker loci, arranged into nine
major linkage groups and four smaller unassigned segments, covering a genetic distance of 1606 cM. Comparison of the B. napus and B. oleracea linkage maps showed that eight out of nine B. oleracea linkage groups were conserved in the B. napus map. There were also regions in the B. oleracea map showing homoeologies with more than one linkage group in the B. napus map. These results provided molecular evidence for B. oleracea, or a closely related 2n=18 Brassica species, as the C-genome progenitor, and also reflected on the homoeology between the A and C genomes in B. napus.
Received: 14 June 1996 / Accepted: 11 October 1996 相似文献
17.
W. C. Kennard R. L. Phillips R. A. Porter A. W. Grombacher 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2000,101(5-6):677-684
We present the first genetic map of wild rice (Zizania palustris L., 2n=2x=30), a native aquatic grain of northern North America. The map is composed principally of previously mapped RFLP (restriction
fragment length polymorphism) genetic markers from rice (Oryza sativa 2n=2x=24). The map is important as a foundation for genetic and crop improvement studies, as well as a reference for genome organization
comparisons among Gramineae species. A comparative mapping approach with rice is especially useful because wild rice is grouped
in the same subfamily, Oryzoideae, and no other mapping comparison has yet been made within the subfamily. As rice is the
reference point for mapping and gene cloning in cereals, establishing a consensus map within the subfamily identifies conserved
and unique regions. The genomes of wild rice and rice differ in total DNA content (wild rice has twice that of rice) and chromosome
pairs (wild rice=15 versus rice=12). The wild rice linkage map reported herein consists of 121 RFLP markers on 16 linkage
groups spanning 1805 cM. Two linkage groups consist of only two markers. Colinear markers were found representing all rice
linkage groups except #12. The majority of rice loci mapped to colinearly arranged arrays in wild rice (92 of 118). Features
of the map include duplication of portions of three rice linkage groups and three possible translocations. The map gives basic
information on the composition of the wild rice genome and provides tools to assist in the domestication of this important
food source.
Received: 25 August 1998 / Accepted: 20 February 1999 (Corrected version. Originally published in TAG 99:793–799) 相似文献
18.
A genetic linkage map for Pinus radiata based on RFLP, RAPD, and microsatellite markers 总被引:7,自引:0,他引:7
M. E. Devey J. C. Bell D. N. Smith D. B. Neale G. F. Moran 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1996,92(6):673-679
A genetic linkage map for radiata pine (Pinus radiata D. Don) has been constructed using segregation data from a three-generation outbred pedigree. A total of 208 loci were analyzed including 165 restriction fragment length polymorphism (RFLP), 41 random amplified polymorphic DNA (RAPD) and 2 microsatellite markers. The markers were assembled into 22 linkage groups of 2 or more loci and covered a total distance of 1382 cM. Thirteen loci were unlinked to any other marker. Of the RFLP loci that were mapped, 93 were detected by loblolly pine (P. taeda L.) cDNA probes that had been previously mapped or evaluated in that species. The remaining 72 RFLP loci were detected by radiata pine probes from a PstI genomic DNA library. Two hundred and eighty RAPD primers were evaluated, and 41 loci which were segregating in a 11 ratio were mapped. Two microsatellite markers were also placed on the map. This map and the markers derived from it will have wide applicability to genetic studies in P. radiata and other pine species. 相似文献
19.
Inter- and intra-genomic homology of the Brassica genomes: implications for their origin and evolution 总被引:7,自引:0,他引:7
M. J. Truco J. Hu J. Sadowski C. F. Quiros 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1996,93(8):1225-1233
In order to determine the homologous regions shared by the cultivated Brassica genomes, linkage maps of the diploid cultivated B. rapa (A genome, n = 10), B. nigra (B genome, n = 8) and B. oleracea (C genome, n = 9), were compared. We found intergenomic conserved regions but with extensitve reordering among the genomes. Eighteen linkage groups from all three species could be associated on the basis of homologous segments based on at least three common markers. Intragenomic homologous conservation was also observed for some of the chromosomes of the A, B and C genomes. A possible chromosome phylogenetic pathway based on an ancestral genome of at least five, and no more than seven chromosomes, was drawn from the chromosomal inter-relationships observed. These results demonstrate that extensive duplication and rearrangement have been involved in the formation of the Brassica genomes from a smaller ancestral genome. 相似文献
20.
Arjula R. Reddy Brian Scheffler G. Madhuri M. N. Srivastava Arvind Kumar P. V. Sathyanarayanan Suresh Nair Madan Mohan 《Plant molecular biology》1996,32(4):735-743
The chalcone synthase is a key enzyme that catalyses the first dedicated reaction of the flavonoid pathway in higher plants. The chs gene and its protein product in rice has been investigated. The presence of a chalcone synthase (CHS) protein in rice seedlings and its developmental stage-specific expression has been demonstrated by western analysis. The chalcone synthase of rice was found to be immunologically similar to that of maize. A rice cDNA clone, Os-chs cDNA, encoding chalcone synthase, isolated from a leaf cDNA library of an indica rice variety Purpleputtu has been mapped to the centromeric region of chromosome 11 of rice. It was mapped between RFLP markers RG2 and RG103. RG2 is the nearest RFLP marker located at a genetic distance of 3.3 cM. Some segments of chromosome 11 of rice including chs locus are conserved on chromosome 4 of maize. The markers, including chs locus on chromosome 11 of rice are located, though not in the same order, on chromosome 4 of maize. Genetic analysis of purple pigmentation in two rice lines, Abhaya and Shyamala, used in the present mapping studies, indicated the involvement of three genes, one of which has been identified as a dominant inhibitor of leaf pigmentation. The Os-chs cDNA shows extensive sequence homology, both for DNA and protein (deduced), to that of maize, barley and also to different monocots and dicots. 相似文献