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1.
Ooplasmic segregation is of great importance in the development of Annelida. The mechanisms of this process are very diverse in different groups of polychaetes, oligochaetes, and leeches (Fernandez et al., 1998). Ooplasmic segregation in Nereis virens is connected with the first meiotic spindle formation and animal-vegetative axis appearance. Spherical polyaxial symmetry of the oocyte transforms into radial stratified symmetry in the course of ooplasmic segregation. There are two main steps of ooplasmic segregation in Nereis virens. The first step begins after the cortical reaction when the central clear cytoplasm reaches the surface of the oocyte. The movement of the cytoplasm is sensitive to nocodazole, colchicine, and cytochalasin B and appears to be mediated by microtubules and, partly, by microfilaments. The second step is not sensitive to the microtubule inhibitors and is mediated mainly by actin filaments. Ooplasmic segregation in Nereis virens may be considered as a primitive form of ooplasmic segregation in Annelida. 相似文献
2.
Ooplasmic segregation in theTubifex egg: Mode of pole plasm accumulation and possible involvement of microfilaments 总被引:1,自引:0,他引:1
Takashi Shimizu 《Development genes and evolution》1982,191(4):246-256
Summary Ooplasmic segregation, i.e. the accumulation of pole plasm in theTubifex egg, consists of two steps: (1) Cytoplasm devoid of yolk granules and lipid droplets migrates toward the egg periphery and forms a continuous subcortical layer around the whole egg; (2) the subcortical cytoplasm moves along the surface toward the animal pole in the animal hemisphere and toward the vegetal pole in the vegetal hemisphere, and finally accumulates at both poles of the egg to form the animal and vegetal pole plasms. Whereas the subcortical layer increases in volume during the first step, it decreases during the second step. This is ascribed to the compact rearrangement in the subcortical layer of membraneous organelles such as endoplasmic reticulum and mitochondria. The number of membraneous organelles associated with the cortical layer increases during the second step. Electron microscopy reveals the presence of microfilaments not only in the cortical layer but also in the subcortical layer. Subcortical microfilaments link membraneous organelles to form networks; some are associated with bundles of cortical microfilaments. The thickness of the cortical layer differs regionally. The pattern of this difference does not change during the second step. On the other hand, the subcortical cytoplasm moves ahead of the stationary cortical layer. The accumulation of pole plasm is blocked by cytochalasin B but not by colchicine. The first step of this process is less sensitive to cytochalasin B than the second step, suggesting that these two steps are controlled by differnt mechanisms. The mechanical aspects of ooplasmic segregation in theTubifex egg are discussed in the light of the present observations. 相似文献
3.
Summary Ooplasmic segregation in ascidians includes the movement of the myoplasm, a pigmented cytoplasmic region thought to be involved in the determination of the embryonic muscle and mesenchyme cell lineages, into the vegetal hemisphere of the egg. A myoplasmic cytoskeletal domain (MCD), composed of a cortical actin network (the PML) and an underlying filamentous lattice extending deep into the cytoplasm, is present in this region. The MCD gradually recedes into the vegetal hemisphere during ooplasmic segregation. It has been proposed that the segregation of the myoplasm is mediated by the contraction of the PML. To test this possibility we have examined ooplasmic segregation in eggs in which the internal parts of the MCD were separated from the PML by centrifugal force. Transmission and scanning electron microscopy of eggs extracted with Triton X-100 showed that the PML remained intact when the internal portions of the MCD were displaced and stratified by centrifugation. When stratified eggs were fertilized there were no rearrangements of the visible cytoplasmic inclusions, but the cellular deformations and the recession of the PML characteristic of ooplasmic segregation occurred as usual. The results indicate that the recession of the PML occurs independently of the internal constituents of the MCD and suggest that PML contraction is the motive force for ooplasmic segregation. 相似文献
4.
M. Zalokar 《Development genes and evolution》1974,175(3):243-248
Summary Ooplasmic segregation inPhallusia mammillata was completed 3 to 5 min after fertilization. Colchicine, which completely stopped nuclear divisions, did not inhibit segregation. Cytochalasin B, which prevented cleavage at a low concentration (0.2 g/ml) inhibited segregation only at a concentration at least five times higher. The action of these drugs leads to the conclusion that ooplasmic segregation does not depend on an assembly of microtubules or on microfilaments which are involved in cell division.This work was performed at the Station Zoologique Villefranche-sur mer (Director: Prof. P. Bougis). The work was supported by a grant A.T.P. of C.N.R.S. 相似文献
5.
Artur G. M. Mattisson 《Histochemistry and cell biology》1965,5(2):97-115
Summary The localisation of succinic dehydrogenase and cytochrome oxidase in body muscles of Nereis virens and in tail muscles of Homarus gammarus was studied. Pig heart muscle was used for some comparisons.Electron microscopic studies on tissue sections generally showed well developed and independent mitochondria in Homarus gammarus. A lower degree of independence characterised the less developed mitochondria of Nereis virens.Sections were stained with nitro-BT. Light microscopic studies showed a distinct and selective staining of the mitochondria in sections of Homarus gammarus. In addition to the few mitochondria of Nereis virens strings within the cytoplasm became distinctly blue. Electron microscopic studies on Nereis virens showed a higher electron density along the membranes of the vesicular sarcotubular system in incubated than in non-incubated sections.The fractions obtained on centrifugation of the homogenised tissues were used for combined enzyme studies and electron microscopic investigation. Similarly prepared fractions from the two invertebrates showed a similar electron microscopic appearance. The supernatants obtained by centrifugation at 12,000 g for 10 minutes contained vesicles different from the majority of those in the mitochondrial fractions. These supernatants had rather considerable activities of succinate-cytochrome c reductase and of cytochrome c oxidase. The activity of succinate-cytochrome c reductase was most pronounced in the supernatants of Nereis virens and much greater than the cytochrome c oxidase activity in these fractions. The ratio between succinate-cytochrome c reductase activity and cytochrome c oxidase activity in the supernatants of Nereis virens was about three times that in the corresponding fractions of Homarus gammarus.Manometric studies were performed to get the effect of added succinate on the O2 uptake of the supernatants obtained by centrifugation at 12,000 g for 10 minutes. A distinctly larger increase in oxygen consumption characterised the supernatants of Nereis virens.The results presented indicate the occurrence of an extra-mitochondrial succinic dehydrogenase in Nereis virens. This conclusion was related to the occurrence of alternative oxidative systems in the muscles of this invertebrate.The literature dealing with an extra-mitochondrial localisation of succinic dehydrogenase is briefly reviewed as well as the electron microscopic studies concerning transformations between the membrane structures of cells. 相似文献
6.
ShotaChiba YukoMiki KazunoriAshida Michiko R.Wada Kimio J.Tanaka YumikoShibata RinnaNakamori TakahitoNishikata 《Development, growth & differentiation》1999,41(3):265-272
Ooplasmic segregation in ascidian eggs consists of two phases of cytoplasmic movement, the first phase is mediated by the microfilament system and the second is mediated by the microtubule system. Recently, two novel proteins, p58 and myoplasmin-C1, which are localized to the myoplasm, were suggested to have important roles in muscle differentiation. In order to analyze the molecular mechanisms underlying ooplasmic segregation, the interactions between actin, tubulin, p58 and myoplasmin-C1 were examined. During the first segregation, microtubule meshwork in the unfertilized egg disappeared. At the second segregation, a novel structure of the microtubules that extended from the sperm aster and localized in the cortical region of the myoplasm was found. Moreover, uniform distribution of the cortical actin filament was observed at the second segregation. During the course of myoplasm rearrangement, p58 and myoplasmin-C1 are colocalized and can form a molecular complex in vitro. This complex of p58 and myoplasmin-C1 is a good candidate for a cytoskeletal component of the myoplasm, and is likely to be involved in the correct distribution of cytoplasmic determinants. 相似文献
7.
A Cytological Analysis of Differentiation Without Cleavage in Cytochalasin B- and Colchicine-Treated Embryos of Chaetopterus pergamentaceus 总被引:1,自引:0,他引:1
WILLIAM R. ECKBERG YUAN-HSU KANG 《Differentiation; research in biological diversity》1981,19(1-3):154-160
Chaetopterus eggs undergo characteristic ooplasmic rearrangements during development. Ooplasmic rearrangement in the absence of cell division is called differentiation without cleavage. Treatment of fertilized eggs with cytochalasin B allowed the continuation of nuclear divisions in the absence of cytoplasmic division. The ooplasmic rearrangements in uncleaved cytochalasin B-treated fertilized eggs closely paralleled those of normal development. Colchicine treatment, which blocks mitosis, arrested ooplasmic movements at a stage comparable to that of normal embryos at first cleavage. Neither drug eliminated the segregation between hyaloplasm and endoplasm, even though colchicine prevented the later rearrangements. Localizing movements are therefore dependent upon normal microtubule function, but not on microfilament function. The maintenance of localized materials does not seem to depend exclusively on either of these organelles. 相似文献
8.
Pattern formation in molluscs is illustrated by the exampleof the larval head pattern of gastropods. The egg cell at thebeginning of development is provided with a spatial patternof developmental factors lying in the surface membrane, by whichthe main axes of the future embryo and the frame of referenceof ooplasmic segregation are determined. Ooplasmic segregationleads to a gradient-like distribution of pole plasm substancealong the main axis and to the formation of RNA-rich granulain the vegetative cells, which play a part in the inductionof bilateral symmetry in the animal hemisphere. The patternof larval head organs arises by interaction of the axial gradientand a dorsoventral concentration gradient of an inductive substance. 相似文献
9.
Shimizu T 《The International journal of developmental biology》1999,43(1):11-18
Annelid embryos are comprised of yolk-deficient animal and yolk-filled vegetal blastomeres. This "unipolar" organization along the animal-vegetal axis (in terms of ooplasmic distribution) is generated via selective segregation of yolk-free, clear cytoplasm to the animal blastomeres. The pathway that leads to the unipolar organization is different between polychaetes and clitellates (i.e., oligochaetes and hirudinidans). In polychaetes, the clear cytoplasm domain, which is established through ooplasmic segregation at the animal side of the egg, is simply cut up by unequal equatorial cleavage. In clitellates, localization of clear cytoplasm to animal blastomeres is preceded by unification of the initially separated polar domains of clear cytoplasm, which result from bipolar ooplasmic segregation. In this article, I have reviewed recent studies on cytoskeletal mechanisms for ooplasmic localization during early annelid development. Annelid eggs accomplish ooplasmic rearrangements through various combinations of three cytoskeletal mechanisms, which are mediated by actin microfilaments, microtubules and mitotic asters, respectively. One of the unique features of annelid eggs isthat a homologous process is driven by distinct cytoskeletal elements. Annelid eggs may provide an intriguing system to investigate not only mechanical aspects of ooplasmic segregation but also evolutionary divergence of cytoskeletal mechanisms that operate in a homologous process. 相似文献
10.
Summary The egg cytoplasm of ascidian,Ciona intestinalis, segregates towards both the animal and vegetal poles within a few minutes of fertilization or parthenogetic activation with ionophore A23187. A constriction appears first on the egg surface near the animal pole and then moves to the vegetal pole. Carmine granules and spermatozoa attached to the egg surface move towards the vegetal pole with the movement of the constriction. Microvilli, which are distributed uniformly in unfertilized egg, disappear on the animal side of the constriction and became more dense on the vegetal side of the constriction. Transmission electron microscopy revealed that sub-cortical cytoplasm, containing numerous mitochondria and sub-cortical granules, moves towards the vegetal pole with the movement of the constriction and then concentrates into a cytoplasmic cap at the vegetal pole. An electron-dense layer appears in the cortex of the cap. The ooplasmic segregation and the cortical contraction were inhibited by cytochalasin B and induced by ionophore A23187. These observations suggest that ooplasmic segregation is caused by the cortical contraction which is characterised by a surface constriction and by the formation of an electron-dense layer. 相似文献
11.
Sergei Vadimovich Evsikov Ludmila Michailovna Morozova Alexander Petrovich Solomko 《Development genes and evolution》1994,203(4):199-204
A new micromanipulation technique permitted the scrambling of the zygote cytoplasm. Such interference had no effect on preimplantation development, and when zygotes with scrambled cytoplasm were transfered to the pseudopregnant females, normal and fertile mice were born. This demonstrates that no morphogenetic factors are prelocalized in the egg cytoplasm. Cleavage characteristics of mouse embryos provide the evidence that zygote cytoplasm does not define any determinate type of cleavage. We conclude that the mechanism of ooplasmic segregation is not used in the mouse (and presumably mammalian) development. It is suggested that the turning point in the evolution of mammalian embryogenesis was the transition to the intrauterine development, that started the process leading among other changes, to the loss of the ooplasmic morphogenetic determinants.
Correspondence to: S.V Evsikov 相似文献
12.
Intracellular Ca2+ transients occur at fertilization in the eggs of all animal species and are thought to be critical for the initiation of several events in egg activation. The rho family of small GTPases are known to organize and maintain the actin filament-dependent cytoskeleton, and rho is involved in the control mechanism of cytokinesis. In the ascidian Ciona savignyi, the first step of ooplasmic segregation observed just after fertilization is cortical contraction with egg deformation, mediated by the cortical actin filaments. C3 exoenzyme, a rho-specific inhibitor, did not affect the pattern of [Ca2+]i transients in the ascidian egg, but inhibited ooplasmic segregation and cytokinesis at the first cleavage. Injection of inositol 1,4,5-trisphosphate or treatment of Ca2+ ionophore induced deformation of the egg and extrusion of the first polar body, but these phenomena did not occur in the C3 exoenzyme-injected egg. These results suggest that rho proteins are involved in egg deformation, ooplasmic segregation and cytokinesis downstream of the [Ca2+]i transients. 相似文献
13.
Ceri Lewis Tamara Galloway 《Mutation Research - Genetic Toxicology and Environmental Mutagenesis》2008,654(1):69-75
The marine environment is becoming increasingly contaminated by environmental pollutants with the potential to damage DNA, with marine sediments acting as a sink for many of these contaminants. Understanding genotoxic responses in sediment-dwelling marine organisms, such as polychaetes, is therefore of increasing importance. This study is an exploration of species-specific and cell-specific differences in cell sensitivities to DNA-damaging agents in polychaete worms, aimed at increasing fundamental knowledge of their responses to genotoxic damage. The sensitivities of coelomocytes from three polychaetes species of high ecological relevance, i.e. the lugworm Arenicola marina, the harbour ragworm Nereis diversicolor and the king ragworm Nereis virens to genotoxic damage are compared, and differences in sensitivities of their different coelomic cell types determined by use of the comet assay. A. marina was found to be the most sensitive to genotoxic damage induced by the direct-acting mutagen methyl methanesulfonate (MMS), and showed dose-dependent responses to MMS and the polycyclic aromatic hydrocarbon benzo(a)pyrene. Significant differences in sensitivity were also measured for the different types of coelomocyte. Eleocytes were more sensitive to induction of DNA damage than amoebocytes in both N. virens and N. diversicolor. Spermatozoa from A. marina showed significant DNA damage following in vitro exposure to MMS, but were less sensitive to DNA damage than coelomocytes. This investigation has clearly demonstrated that different cell types within the same species and different species within the polychaetes show significantly different responses to genotoxic insult. These findings are discussed in terms of the relationship between cell function and sensitivity and their implications for the use of polychaetes in environmental genotoxicity studies. 相似文献
14.
Quantitative analysis of cellular differentiation during early embryogenesis ofPlatynereis dumerilii
Adriaan W. C. Dorresteijn 《Development genes and evolution》1990,199(1):14-30
Summary As in many spiralian embryos with unequal cleavage, cleavage inPlatynereis follows an invariant pattern. Preceding each cleavage the cytoplasm is reorganized, allowing the spiral cleavage mode to produce cells with different cytoplasmic composition. The fertilized egg undergoes a dramatic ooplasmic segregation after the completion of the cortical reaction. As a consequence, a plug of clear cytoplasm becomes located at the animal pole. Once the four quadrants of the embryo have been established, the cleavage sequence of the D quadrant differs clearly from that of the other three quadrants. The results presented here suggest that differential distribution of the clear cytoplasm governs this sequence. The first quartet of micromeres, which will form the ectoderm and the cerebral ganglia of the head, is clearly bilaterally symmetrical from the onset of the third cleavage. Dorsoventral polarity and bilateral symmetry in the ectoderm of the trunk is expressed most markedly by the dorsal location of the large 2d cell, whose rapid proliferation is bilaterally symmetrical with respect to the median plane. As a result of this proliferation it comes to fill most of the posttrochal region (ectoderm, three pairs of anlagen for the setal sacs, and the ventral plate which forms the nerve cord). The other micromeres contribute only a minor portion of the ventral ectoderm and are involved in the formation of the stomodaeum. The mesentoblast, 4d, i.e. the stem cell of the primary mesoderm, forms at the sixth cleavage, also in a position on the dorsal mid-line. The daughter cells, which arise from 4d by strictly bilaterally symmetrical cleavage, form the mesodermal germ bands, which lie beneath the ectoderm. The trochoblasts are formed by asynchronously cleaving founder cells, but further cleavages in these cells are synchronous. This suggests that cell-cell interaction is involved in the development of this alleged mosaic embryo. 相似文献
15.
《Developmental biology》1986,116(1):241-251
The role of the cortex in ooplasmic segregation of the yolky eggs of Tubifex has been studied by epifluorescence microscopy. Living eggs labeled with rhodamine 123 and fine carbon particles placed on the surface showed that, following the second polar body formation, the egg surface cosegregates with subcortical mitochondria in a bipolar fashion, viz. toward the animal and vegetal poles in the animal and vegetal hemispheres, respectively. The egg surface of each pole moves spirally while the equatorial surface appears to remain stationary during this process. The rhodamine-phalloidin staining of whole eggs reveals that actin networks cosegregate with mitochondria. Isolated cortices which were stained with rhodamine-phalloidin demonstrated that cortical actin is organized bipolarly and that, during ooplasmic segregation, it undergoes reorganization directed toward both poles of the egg. The cortical polarity expressed as actin organization is not disrupted by centrifugal force sufficient to stratify the egg cytoplasm into five layers. The surface of a centrifuged egg moves according to the original cortical polarity. This surface movement is accompanied by the reorganization of cortical actin which appears to be identical to that in intact eggs. Other centrifugation experiments have demonstrated that the connection of the subcortical cytoplasm to the cortex is resistant to a centrifugal force of up to 650g. The nature of cortical polarity and its role in ooplasmic segregation are discussed in the light of the present results. 相似文献
16.
Markus Henzen 《Cell and tissue research》1966,71(3):415-440
Summary The egg of the annelid Tubifex reveals, during its meiosis, a change of inner structure which leads to the formation of a developmental pattern. By means of cytological and microcytological technique, the segregation of the different cytoplasmic particles into a specific pattern has been investigated. The results are as follows:The cytoplasm of the mature egg contains as components a hyaloplasm mixed with biosomatic elements and different nutritive particles and parallely or radially oriented fibrils. During meiosis biosomatic elements — endoplasmic vesicles and mitochondria — as well as nutritive particles — lipid droplets and yolk granules — migrate within the cell in a typical streaming movement. As a result of this ooplasmic segregation, the particles arrange themselves before the first mitotic division in a specific morphogenetic pattern; the most significant arrangement of cytoplasmic particles consists of mitochondria and endoplasmic vesicles forming the two polar plasms. It is possible to trace back the formation of these morphogenetic plasms to the beginning of meiosis.Nothing is known about physico-chemical factors which might influence the ooplasmic segregation. It might be relevant, however, that all particles taking part in this morphogenetic movement, are coated with a thin plasmic membrane.
Ausgeführt mit Beiträgen der Eidgenössischen Kommission zur Förderung der wissenschaftlichen Forschung aus Arbeitsbeschaffungskrediten des Bundes. 相似文献
Ausgeführt mit Beiträgen der Eidgenössischen Kommission zur Förderung der wissenschaftlichen Forschung aus Arbeitsbeschaffungskrediten des Bundes. 相似文献
17.
Andreeva T. F. Kuk Ch. Korchagina N. M. Eikem M. Dondua A. K. 《Russian Journal of Developmental Biology》2001,32(3):183-191
We have undertaken an active search for homeobox-containing sequences of Antpclass (Hoxgenes) in the genome DNA of polychaete Nereis virens. This search was based on the high evolutionary conservation of these sequences, which made possible their amplification in the polymerase chain reaction with degenerate primers. As a result, eleven fragments of various Hoxgenes, including AbdB-like Nvi-post1, were cloned. Using pulsed-field electrophoresis, we have demonstrated that Hoxgenes corresponding to the isolated fragments are clustered in the genome of N. virens. 相似文献
18.
The effects of microtubule and microfilament inhibitors on ooplasmic segregation and microtubule organization were examined during fertilization, parthenogenetic activation, and early development in the ascidian Molgula occidentalis. At fertilization the egg cortex contracts as the first phase movement and shortly after mitochondria migrate as the myoplasmic crescent develops in the second phase. The microtubule inhibitors colcemid and nocodazole inhibit the second phase, but not the first phase, of ooplasmic segregation. The microfilament inhibitor cytochalasin E has the reciprocal effect of inhibiting the first, but not the second, phase. It appears that sperm may initially bind at any site on the egg surface and that the contractile activities at the first phase and during polar body formation occur independent of the microtubule system. Since the second phase migration occurs as the sperm astral microtubules assemble and since microtubule, but not microfilament, inhibitors arrest this aspect of ooplasmic segregation, microtubules appear necessary for mitochondrial migration. These results demonstrate that the two phases of ascidian ooplasmic segregation are mediated by different systems, the first by microfilaments and the second by microtubules. The microtubule and microfilament systems appear to operate independent of one another and their combined actions result in the completion of ooplasmic segregation. A model is proposed in which the cortical contraction following fertilization is important not only as the motive force for the first phase movement but also as a method to unite the myoplasm with the entering sperm which can initially bind anywhere on the egg surface. The association between myoplasmic components and the growing sperm aster would ensure that the migration and the spatial distribution of myoplasm in the second phase results in the formation of the myoplasmic crescent. 相似文献
19.
Dynamics of the actin microfilament system in the Tubifex egg during ooplasmic segregation 总被引:2,自引:0,他引:2
T Shimizu 《Developmental biology》1984,106(2):414-426
Following the second polar body formation (PBF), the Tubifex egg undergoes ooplasmic segregation consisting of two steps, i.e., centrifugal migration of membranous organelles forming a subcortical ooplasmic layer and then movements of these organelles along the egg surface. The present investigation was undertaken to examine the microfilament organization in eggs during these ooplasmic rearrangements. Microfilaments throughout the egg are identified as actin by their reversible heavy meromyosin binding. Before the second PBF, a distinct network of actin filaments is present in the endoplasmic region. It is disorganized during the second PBF; short actin filaments are caused to aggregate with membranous organelles. Following the second PBF, similar short filaments become localized in the subcortical layer but not in the underlying yolky region. However, it is not until 50-60 min after the second PBF that an elaborate actin network is established in the subcortical layer. The cortex contains a sheet-like lattice of actin filaments. It is thickest around the animal pole, and tapes toward the equator of the egg. At about 90 min after the second PBF, this polarized distribution of cortical filaments becomes more pronounced as the result of their movements. Chronologically, subcortical actin network formation and cortical reorganization correspond to the later portion of the first step and the earlier portion of the second step of ooplasmic segregation, respectively. These findings are discussed in terms of ooplasmic movements and rearrangements. 相似文献
20.
MARK MCMENAMIN 《Invertebrate reproduction & development.》2013,57(2-3):165-166
Summary The role of Ca2+ in activation and early development of locust eggs was examined through measurement of ooplasmic Ca2+ levels before and after fertilization, and through experimental activation of unfertilized eggs. Ooplasmic pCa (i.e. the negative logarithm of Ca2+ activity) measured in intact eggs decreased from 5.35 before fertilization, to 4.77 and 3.00 by 1 day and 3 days after fertilization, respectively. pCa was also determined for samples of ooplasm collected by rupturing eggs under paraffin oil. The pCa was 5.10 in ooplasm isolated from unfertilized eggs, and 3.84 in ooplasm collected from eggs within 4 h of fertilization. Ooplasmic pCa remained between 3.97 and 3.12 from 1–6 days after fertilization. Since a decline in pCa indicates an increase in ooplasmic Ca2+ activity, the data suggest that regulation of ooplasmic Ca2+ during post-fertilization development involves release of Ca2+ from internal stores. Experimental egg activation was examined in eggs dissected from the oviducts before fertilization and incubated on moist filter paper. Some eggs were first immersed in experimental solutions for 30–60 minutes before incubation. The presence of an embryo 2 or 4 days after fertilization or experimental treatment was used as an indicator of egg activation. Activation occurred in 92% and 12% of fertilized and untreated eggs, respectively. The percentage of unfertilized eggs which activated increased to 47% if eggs were soaked 30–60 minutes in physiological saline, and to as much as 65%-68% if eggs were injected with Ca2+ buffers or if a Ca2+ action potential was evoked. Up to 36% and 42% of unfertilized eggs activated after incubation in Ca2+-free salines or in the presence of the Ca2+-channel blocker Cd2+, respectively. Taken together, the results suggest that entry of external Ca2+ through voltage dependent channels increases the proportion of eggs which activate, but is not an absolute requirement for activation. 相似文献