共查询到20条相似文献,搜索用时 15 毫秒
1.
2.
Julien Boudet Anne Chouquet Aicha Chahboune Cécile Giustini Bernard Joris Jean-Pierre Simorre Catherine Bougault 《Biomolecular NMR assignments》2007,1(1):89-91
The ampG gene codes for a permease required to uptake anhydro-muropeptides into bacterial cytoplasm. Located upstream in the same
operon, is another 579-base-pair-long open reading frame encoding a putative lipoprotein YajG, whose nearly complete 1H,13C,15N assignments are reported here. 相似文献
3.
Lipocalin2 plays an important role in the innate immune system. In this article we report the backbone and side-chain resonance
assignments of rat lipocalin2 (rLcn2). These assignments provide a basis for determining the structure and dynamics of rLcn2.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users. 相似文献
4.
Richard Harris Ulf Eidhoff Daniela Vinzenz Martin Renatus Bernd Gerhartz Ulrich Hommel Paul C. Driscoll 《Biomolecular NMR assignments》2007,1(1):51-53
To facilitate NMR spectroscopy studies of interactions with various ligands and potential inhibitors, we report the NMR backbone
resonance assignments for the 26 kD human enzyme UCH-L3, a member of the ubiquitin C-hydrolase family of ubiquitin-specific
cysteine proteases. 相似文献
5.
6.
Kozlov G Cheng J Lievre C Banville D Gehring K Ekiel I 《Journal of biomolecular NMR》2002,24(2):169-170
7.
The carboxy-terminal pleckstrin homology (PH) domain recruits GRP1 to the plasma membrane through the specific binding to
phosphatidylinositol 3,4,5-trisphosphate [PtdIns(3,4,5)P3]. Here, we describe backbone and side chain assignments of the GRP1 PH domain determined by triple resonance experiments.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users. 相似文献
8.
9.
10.
Yunchen Bi Hongwei Li Shoujin Fan Bin Xia Changwen Jin 《Biomolecular NMR assignments》2009,3(1):149-151
The mRNA degradation is an important regulatory mechanism which controls gene expression by limiting the number of translation
times. Previous studies demonstrated that this process is essential for organisms. Escherichia coli RNA pyrophosphohydrolase (RppH) is an enzyme that triggers mRNA degradation by removing the 5′ pyrophosphate, which is a
rate-determining step. In order to understand the molecular mechanism of the biological function, the structural information
of RppH is required. Herein, we report the resonance assignments of 1H, 15N, 13C atoms of the E. coli RppH. 相似文献
11.
Christopher J. Falzone John Cavanagh Marlon Cowart Arthur G. Palmer III C. Robert Matthews Stephen J. Benkovic Peter E. Wright 《Journal of biomolecular NMR》1994,4(3):349-366
Summary By using fully 15N- and 15N/13C-labeled Escherichia coli dihydrofolate reductase, the sequence-specific 1H and 15N NMR assignments were achieved for 95% of the backbone resonances and for 90% of the 13C resonances in the binary folate complex. These assignments were made through a variety of three-dimensional proton-detected 15N and 13C experiments. A smaller but significant subset of side-chain 1H and 13C assignments were also determined. In this complex, only one 15N or 13C resonance was detected per 15N or 13C protein nucleus, which indicated a single conformation. Proton-detected 13C experiments were also performed with unlabeled DHFR, complexed with 13C-7/13C-9 folate to probe for multiple conformations of the substrate in its binary complex. As was found for the protein resonances, only a single bound resonance corresponding to a productive conformation could be detected for C-7. These results are consistent with an earlier report based on 1H NMR data [Falzone, C.J. et al. (1990) Biochemistry, 29, 9667–9677] and suggest that the E. coli enzyme is not involved in any catalytically unproductive binding modes in the binary complex. This feature of the E. coli enzyme seems to be unique among the bacterial forms of DHFR that have been studied to date. 相似文献
12.
13.
14.
15.
16.
17.
Sagaya Theresa Leena Philominathan Osamu Matsushita John Brad Jordan Joshua Sakon 《Biomolecular NMR assignments》2008,2(2):127-129
1H, 15N, and 13C NMR assignments for 116 amino acids (Gly893-Lys1008) of a bacterial collagen-binding domain (CBD) derived from Clostridium histolyticum class I collagenase were accomplished. Clostridial collagenases hydrolyze insoluble collagen. One to three copies of collagen-binding
domains (CBDs) are present at their C-termini, each of which is the minimal segment required for the binding to the insoluble
substrate. CBD has been shown to be able to anchor fused growth factors for up to 10 days in vivo. Structural analysis of
the small domain with the unique function provides insights into designing a novel drug delivery vehicle by the rational drug
design. 相似文献
18.
19.
Summary Sequence-specific 1H, 13C and 15N resonance assignments have been established for rat intestinal fatty acid-binding protein complexed with palmitate (15.4 kDa) at pH 7.2 and 37°C. The resonance assignment strategy involved the concerted use of seven 3D triple-resonance expriments (CC-TOCSY, HCCH-TOCSY, HNCO, HNCA, 15N-TOCSY-HMQC, HCACO and HCA(CO)N). A central feature of this strategy was the concurrent assignment of both backbone and side-chain aliphatic atoms, which was critical for overcoming ambiguities in the assignment process. The CC-TOCSY experiment provided the unambiguous links between the side-chain spin systems observed in HCCH-TOCSY and the backbone correlations observed in the other experiments. Assignments were established for 124 of the 131 residues, although 6 of the 124 had missing amide 1H resonances, presumably due to rapid exchange with solvent under these experimental conditions. The assignment database was used to determine the solution secondary structure of the complex, based on chemical shift indices for the 1H, 13C, 13C and 13CO atoms. Overall, the secondary structure agreed well with that determined by X-ray crystallography [Sacchettini et al. (1989) J. Mol. Biol., 208, 327–339], although minor differences were observed at the edges of secondary structure elements. 相似文献