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Draženka Komes Ana Belščak‐Cvitanović Dunja Horžić Gordana Rusak Saša Likić Marija Berendika 《Phytochemical analysis : PCA》2011,22(2):172-180
Introduction – Polyphenolic phytochemicals in traditionally used medicinal plants act as powerful antioxidants, which aroused an increasing interest in their application in functional food development. Objective – The effect of extraction time (5 and 15 min) and hydrolysis on the qualitative and quantitative content of phenolic compounds and antioxidant capacity of six traditionally used medicinal plants (Melissa officinalis L., Thymus serpyllum L., Lavandula officinalis Miller, Rubus fruticosus L., Urtica dioica L., and Olea europea L.) were investigated. Methodology – The content of total phenols, flavonoids, flavan‐3‐ols and tannins was determined using UV/Vis spectrophotometric methods, while individual phenolic acids, flavones and flavonols were separated and detected using HPLC analysis. Also, to obtain relevant data on the antioxidant capacity, two different assays, (2,2‐azino‐bis(3‐ethylbenzthiazoline‐6‐sulphonic acid) (ABTS) radical scavenging and ferric reducing/antioxidant power (FRAP) assays were used. Results – The extraction efficiency of phenolics, as well as the antioxidant capacity of plant extracts, was affected by both prolonged extraction and hydrolysis. The overall highest content of phenolic compounds was determined in hydrolyzed extract of blackberry leaves (2160 mg GAE/L), followed by the non‐hydrolyzed extract of lemon balm obtained after 15 min of extraction (929.33 mg GAE/L). The above extracts also exhibited the highest antioxidant capacity, while extracts of olive leaves were characterized with the lowest content of phenolic compounds, as well as the lowest antioxidant capacity. The highest content of rosmarinic acid, as the most abundant phenolic compound, was determined in non‐hydrolyzed extract of lemon balm, obtained after 15 min of extraction. Although the hydrolysis provided the highest content of polyphenolic compounds, longer extraction time (15 min) was more efficient to extract these bioactives than shorter extraction duration (5 min). Conclusion – The distribution of detected phenolic compounds showed a wide variability with regard to their botanical origin. Examined medicinal plants showed to be a valuable supplement to a daily intake of bioactive compounds. Copyright © 2010 John Wiley & Sons, Ltd. 相似文献
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Compositions of phenolic substances were studied in leaves of 21 species of medicinal plants. Flavonoid levels varied from 1.94 to 5.42%, whereas total amounts of monomeric polyphenols and hydroxybenzoic acids were estimated as 0.27 to 0.57%, and hydroxycinnamic acids and their esters with quinic acid, 0.09 to 0.18%. Condensed and polymerized polyphenols were detected in amounts of 0.41 to 1.20%. Qualitative compositions of flavonoids in leaves of seven plants studied were presented. The developed analytical procedures may be useful for plant polyphenol studies and as the basis of hemotaxonomy. 相似文献
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Mahidol Chulabhorn Prawat Hunsa Prachyawarakorn Vilailak Ruchirawat Somsak 《Phytochemistry Reviews》2002,1(3):287-297
It has been estimated that plants are the most important source of medicine for more than 80% of the world’s population. Medicinal
plants are a vital source of medication in developing countries. Despite the wealth of human experience and folklore concerning
the medicinal uses of plants, proper scientific investigation has only been applied to a small fraction of the world’s plants.
This is a cause of grave concern as plant species continue to disappear. A rapid response to this situation is urgently needed
to prevent the disappearance of the plant species and the ethnopharmacological knowledge that accompanies them. In this review,
recent work on the investigation of selected bioactive Thai medicinal plants is presented. Their biological activities against
infectious diseases including antimalarial and anti-HIV, are highlighted, as well as their anticancer, antiulcer and anti-inflammatory
properties. The chemical transformations of some selected compounds are discussed.
This revised version was published online in June 2006 with corrections to the Cover Date. 相似文献
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Michael A. Weiner 《Economic botany》1970,24(3):279-282
The uses of 26 medicinal plants found in Namosi Village, Viti Levu are listed. Included are the plant parts used, Namosi names, the illnesses, and the preparations of the remedies. 相似文献
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Medicinal plants and their endophytes are important resources for discovery of natural products. Several previous studies
have found a positive correlation between total antioxidant capacity (TAC) and total phenolic content (TPC) of many medicinal
plant extracts. However, no information is available on whether such a relationship also exists in their endophytic fungal
metabolites. We investigated the relationship between TAC and TPC for 292 morphologically distinct endophytic fungi isolated
from 29 traditional Chinese medicinal plants. The antioxidant capacities of the endophytic fungal cultures were significantly
correlated with their total phenolic contents, suggesting that phenolics were also the major antioxidant constituents of the
endophytes. Some of the endophytes were found to produce metabolites possessing strong antioxidant activities. Several bioactive
constituents from the fungal cultures and host plant extracts were identified. This investigation reveals that the metabolites
produced by a wide diversity of endophytic fungi in culture can be a potential source of novel natural antioxidants. 相似文献
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Saleem A Ahotupa M Pihlaja K 《Zeitschrift für Naturforschung. C, Journal of biosciences》2001,56(11-12):973-978
Thirty-seven plant organs, traditionally used as drugs, collected in Pakistan, were extracted with 70% acetone and analyzed for their total phenolics concentration and antioxidant potential. Seven extracts showed more than 85% inhibition of lipid peroxidation in vitro as compared with blank. Butylated hydroxytoluene (BHT) (IC50 = 233.6 microg/l +/- 28.3) was the strongest antioxidant in our test system. The IC50 results indicate that the extracts of Nymphaea lotus L. flowers, Acacia nilotica (Linn.) Delile beans, Terminalia belerica Roxb. fruits, and Terminalia chebula Retz. (fruits, brown) were stronger antioxidants than alpha-tocopherol, while Terminalia chebula Retz. (fruit coat), Terminalia chebula Retz. (fruits, black) and Ricinus communis L. leaves were weaker antioxidant extracts than alpha-tocopherol and BHT. Total phenolics concentration, expressed as gallic acid equivalents, showed close correlation with the antioxidant activity. High performance liquid chromatographic analysis with diode array detection at 280 nm, of the seven extracts indicated the presence of hydroxybenzoic acid derivatives, hydroxycinnamic acid derivatives, flavonol aglycones and their glycosides as main phenolics compounds. This information, based on quick screening methods, enables us to proceed towards more detailed chemical and pharmacological understanding of these plant materials. 相似文献
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de Omena MC Navarro DM de Paula JE Luna JS Ferreira de Lima MR Sant'Ana AE 《Bioresource technology》2007,98(13):2549-2556
Larvicidal activities against Aedes aegypti have been determined in the ethanolic extracts obtained from 51 Brazilian medicinal plants. Eleven of the 84 extracts studied showed significant (LC50 < 100 microg mL(-1)) activities against larvae, with extracts from Annona crassiflora (root bark, LC50 = 0.71 microg mL(-1); root wood, LC50 = 8.94 microg mL(-1)) and Annona glabra (seed, LC50 = 0.06 microg mL(-1)) showing the highest activities. The results obtained should be of value in the search for new natural larvicidal compounds. 相似文献
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Boyom FF Ngouana V Zollo PH Menut C Bessiere JM Gut J Rosenthal PJ 《Phytochemistry》2003,64(7):1269-1275
In a search for new plant-derived biologically active compounds against malaria parasites, five essential oils extracted from the Cameroonian plants Xylopia phloiodora, Pachypodanthium confine, Antidesma laciniatum, Xylopia aethiopica, and Hexalobus crispiflorus were evaluated in regard to their anti-plasmodial activity against the W2 strain of Plasmodium falciparum. The oils were obtained from the plants with 0.12, 0.13, 0.18, 0.6 and 0.1% yields (relatively to dried material weight) respectively. Analysis by gas chromatography and mass spectrometry identified mainly terpenoids, among which alpha-copaene, gamma-cadinene, delta-cadinene, alpha-cadinol, spathulenol and caryophyllene oxide were most commonly found. The five oils were active against Plasmodium falciparum in culture. The most effective was the oil of Hexalobus crispiflorus, with an IC50 of 2 microg/ml. 相似文献
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Sanjay Guleria A. K. Tiku Gurjinder Singh Apurva Koul Sahil Gupta Subhash Rana 《Journal of plant biochemistry and biotechnology.》2013,22(1):9-15
Antioxidant activities and phenolic contents of 26 species extracts from 20 botanical families grown in north-western Himalaya were investigated. Antioxidant activities were determined using DPPH (1,1-diphenyl-2-picrylhydrazyl) radical scavenging and ferric reducing antioxidant power (FRAP) assays. Total phenolic content (TPC) was determined using a Folin-Ciocalteu assay. Quantitative and qualitative analysis of phenolic compounds was also carried out by reverse phase high performance liquid chromatography (RP-HPLC) using diode array detector (DAD). Major phenolics determined using RP-HPLC in analyzed species were gallic acid, chlorogenic acid, p-hydroxy benzoic acid, caffeic acid, vanillic acid, syringic acid, p-coumaric acid and ferulic acid. Antiradical efficiency (1/EC50) determined using DPPH radical scavenging assay ranged from 0.13 to 5.46. FRAP values ranged from 8.66 to 380.9 μmol Fe(II)/g dw. Similarly, the total phenolic content in the analyzed species varied from 3.01 to 69.96 mg of gallic acid equivalents (GAE)/g dry weight. Gallic acid was found in the majority of the samples, being most abundant compound in Syzygium cumini bark (92.64 mg/100 g dw). Vanillic acid was the predominant phenolic compound in Picrorhiza kurroa root stolen (161.2 mg/100 g dry weight). The medicinal plants with highest antioxidant activities were Taxus baccata and Syzygium cumini. A significant positive correlation, R 2?=?0.9461 and R 2?=?0.9112 was observed between TPC determined using Folin-Ciocalteu method and antiradical efficiency and FRAP values respectively, indicating that phenolic compounds are the major contributor of antioxidant activity of these medicinal plants. 相似文献
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《Phytomedicine》2020
BackgroundIdentification of the low abundance of phytochemicals in plant extracts is very difficult. Pharmacological activity observed in such plants is not due to a single compound. In most cases, plant extracts show activity based on synergistic or antagonistic effects. Therefore, the idea of a holistic approach is more rational.PurposeThis study was planned to compare the metabolomics and proteomics profiles of Valeriana officinalis L. (Valerianaceae), Melissa officinalis L. (Lamiaceae), Hypericum perforatum L. (Hypericaceae) and Passiflora incarnata L. (Passifloraceae) used in sedative anxiolytic and sleep disorders. Integrated omics analyses were used to provide a better understanding of the effect of plant extracts on the brain-derived neurotrophic factor (BDNF) expression levels on the SH-SY5Y cell line by a holistic approach.MethodsMetabolomic profiling of the plants was performed using the GC–MS and LC-qTOF-MS systems, and the proteomics analysis using the LC-qTOF-MS system after trypsin digestion. The Human BDNF Quantikine ELISA kit was utilized to test BDNF expression activity on the SH-SY5Y cell line.ResultsThe investigated plant extracts showed a significant increase in BDNF expression (p < 0.05). M. officinalis was found as the most active extract. According to the correlation analyses between BDNF activity and metabolomics or proteomics level, 94 metabolites had a positive correlation while 23 metabolites had a highly negative correlation; those for proteins are 24 and 6, respectively.ConclusionThe multivariate data analysis revealed a similar metabolomics profile of H. perforatum and P. incarnata, which also had a similar activity profile. Remarkably, all the primary metabolites belonging to the Krebs Cycle (citric acid, fumaric acid, succinic acid, pyruvic acid, malic acid and citramalic acid, an analog of malic acid) were positively correlated with BDNF activity. Secondary metabolites with a high BDNF expression belonged to flavonoids, xanthone, coumarines, tannin, naphtalenes, terpenoids and carotenoid skeleton. Two proteins from the cytochrome P450 family (P450 71B11 and P450 94B3) were positively correlated with BDNF activity. Employing omics technologies in the plant research area will offer a better understanding of the role of plant extracts and may lead to the discovery of new compounds with specific activity. 相似文献
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In-Kyoung Lee Myung-Suk Han Myeong-Seok Lee Young-Sook Kim Bong-Sik Yun 《Bioorganic & medicinal chemistry letters》2010,20(18):5459-5461
During the search for natural antioxidants from basidiomycetes, a new styrylpyrone, baumin (1), was isolated from the cultivated medicinal fungus Phellinus baumii, together with known compounds, davallialactone (2), hispidin (3), hypholomine B (4), interfungin A (5), inoscavin A (6), and phelligridin D (7), which were previously isolated from the medicinal fungi Phellinus ignarius, Phellinus linteus, and Inonotus xeranticus. Their structures were elucidated by extensive spectroscopic methods. These compounds exhibited antioxidant activity through Fenton reaction inhibition via iron chelation and free radical scavenging. 相似文献
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Merajuddin Khan Mujeeb Khan Syed F. Adil Hamad Z. Alkhathlan 《Saudi Journal of Biological Sciences》2022,29(3):1801-1807
Phytochemicals from plant extracts belong to an important source of natural products which have demonstrated excellent cytotoxic activities. However, plants of different origins exhibit diverse chemical composition and bioactivities. Therefore, discovery of plants based new anticancer agents from different parts of the world is always challenging. In this study, methanolic extracts of different parts of 11 plants from Saudi Arabia have been tested in vitro for their anticancer potential on human liver cancer cell line (HepG2). Particularly, for this study, plants from Asteraceae, Resedaceae and Polygonaceae families were chosen on the basis of locally available ethnobotanical data and their medicinal properties. Among 12 tested extract samples, three samples obtained from Artemisia monosperma stem, Ochradenus baccatus aerial parts and Pulicaria glutinosa stem have demonstrated interesting cytotoxic activities with a cell viability of 29.3%, 28.4% and 24.2%, respectively. Whereas, four plant extracts including Calendula arvensis aerial parts, Scorzonera musilii whole plant, A. monosperma leaves show moderate anticancer properties bearing a cell viability ranging from 11.9 to 16.7%. The remaining extracts have shown poor cytotoxic activities. Subsequently, GC-MS analysis of methanolic extracts of four most active plants extracts such as C. comosum, O. baccatus, P. glutinosa and A. monosperma detected the presence of 41 phytomolecules. Among which 3-(4-hydroxyphenyl) propionitrile (1), 8,11-octadecadiynoic acid methyl ester (2), 6,7-dimethoxycoumarin (3) and 1-(2-hydroxyphenyl) ethenone (4) were found to be the lead compounds of C. comosum, O. baccatus P. glutinosa and A. monosperma, respectively. 相似文献
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Kuete V Wabo HK Eyong KO Feussi MT Wiench B Krusche B Tane P Folefoc GN Efferth T 《PloS one》2011,6(8):e21762