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1.
Martin Sievers Christoph Gaberthüel Cornelia Boesch Wolfgang Ludwig Michael Teuber 《FEMS microbiology letters》1995,126(2):123-126
Abstract The 16S rRNA sequences from the Gluconobacter species G. asaii G. cerinus and G. frateurii were determined and compared with homologous sequences from published databases and sequences of G. oxydans and Acetobacter species previously described [Sievers M., Ludwig W. and Teuber M. (1994) System. Appl. Microbiol. 17, 189–196]. The Gluconobacter species have unique 16S rRNA sequences and exhibit sequence similarity values of 97.4 to 99.1%, corresponding to 36 to 14 base differences. The phylogenetic tree inferring methods (distance matrix, maximum parsimony and maximum likelihood) show that the species of Gluconobacter form a coherent, closely related cluster. Based on the distance matrix method including Rhodopila globiformis as an outgroup reference organism, Gluconobacter is well separated from Acetobacter . 相似文献
2.
3.
The systematic position of the genus Cryothecomonas has been determined from an analysis of the nuclear-encoded small subunit ribosomal RNA gene of Cryothecomonas longipes and two strains of Cryothecomonas aestivalis. Our phylogenetic trees inferred from maximum likelihood, distance and maximum parsimony methods robustly show that the genus Cryothecomonas clusters within the phylum Cercozoa, and is related to the sarcomonad flagellate Heteromita globosa. Morphological data supporting the taxonomic placement of Cryothecomonas near the sarcomonad flagellates has been compiled from the literature. The high number of nucleotide substitutions found between two morphologically indistinguishable strains of Cryothecomonas aestivalis suggests the possibility of cryptic species within Cryothecomonas aestivalis. 相似文献
4.
Mitochondrial small subunit ribosomal DNAs of Cerrena unicolor and Trametes consors were sequenced and compared with those of known mushroom taxa. Trametes consors is a species recently transferred from Irpex, and Cerrena is a genus closely related to Trametes. The present phylogenetic tree showed that Cerrena unicolor and Trametes consors clustered together and made an independent lineage from the Trametes group. A new combination, Cerrena consors (Berk.) Ko and Jung, comb. nov., is proposed here by transferring Trametes consors into Cerrena based on molecular data along with taxonomic evidence. 相似文献
5.
Background
Candida albicans is a polymorphic fungus causing serious infections in immunocompromised patients. It is capable of shifting from yeast to germinating forms such as hypha and pseudohypha in response to a variety of signals, including mammalian serum. We have previously shown that some of the large 25S components of ribosomal RNA in Candida albicans get polyadenylated, and this process is transiently intensified shortly after serum exposure just prior to the appearance of germination changes. 相似文献6.
L. Hendriks R. De Baere Y. Van de Peer J. Neefs A. Goris R. De Wachter 《Journal of molecular evolution》1991,32(2):167-177
Summary The complete small ribosomal subunit RNA (srRNA) sequence was determined for the red algaPorphyra umbilicalis and the basidiomyceteLeucosporidium scottii, representing two taxa for which no srRNA sequences were hitherto known. These sequences were aligned with other published
complete srRNA sequences of 58 eukaryotes. Evolutionary trees were reconstructed by a matrix optimization method from a dissimilarity
matrix based on sections of the alignment that correspond to structurally conservative areas of the molecule that can be aligned
unambiguously. The overall topology of the eukaryotic tree thus constructed is as follows: first there is a succession of
early diverging branches, leading to a diplomonad, a microsporidian, a euglenoid plus kinetoplastids, an amoeba, and slime
molds. Later, a nearly simultaneous radiation seems to occur into a number of taxa comprising the metazoa, the red alga, the
sporozoa, the higher fungi, the ciliates, the green plants, plus some other less numerous groups. Because the red alga diverges
late in the evolutionary tree, it does not seem to represent a very primitive organism as proposed on the basis of morphological
and 5S rRNA sequence data.
Asco- and basidiomycetes do not share a common ancestor in our tree as is generally accepted on the basis of conventional
criteria. In contrast, when all alignment positions, rather than the more conservative ones, are used to construct the evolutionary
tree, higher fungi do form a monophyletic cluster. The hypothesis that higher fungi and red algae might have shared a common
origin has been put forward. Although the red alga and fungi seem to diverge at nearly the same time, no such relationship
can be detected.
The newly determined sequences can be fitted into a secondary structure model for srRNA, which is now relatively well established
with the exception of uncertainties in a number of eukaryote-specific expansion areas. A specific structural model featuring
a pseudoknot is proposed for one of these areas. 相似文献
7.
Phylogenetic analysis of ten black yeast species using nuclear small subunit rRNA gene sequences 总被引:5,自引:0,他引:5
G. Haase L. Sonntag Y. van de Peer J. M. J. Uijthof A. Podbielski B. Melzer-Krick 《Antonie van Leeuwenhoek》1995,68(1):19-33
The nuclear small subunit rRNA genes of authentic strains of the black yeastsExophiala dermatitidis, Wangiella dermatitidis, Sarcinomyces phaeomuriformis, Capronia mansonii, Nadsoniella nigra var.hesuelica, Phaeoannellomyces elegans, Phaeococcomyces exophialae, Exophiala jeanselmei var.jeanselmei andE. castellanii were amplified by PCR and directly sequenced. A putative secondary structure of the nuclear small subunit rRNA ofExophiala dermatitidis was predicted from the sequence data. Alignment with corresponding sequences fromNeurospora crassa andAureobasidium pullulans was performed and a phylogenetic tree was constructed using the neighbor-joining method. The obtained topology of the tree was confirmed by bootstrap analysis. Based upon this analysis all fungi studied formed a well-supported monophyletic group clustering as a sister group to one group of the Plectomycetes (Trichocomaceae and Onygenales). The analysis confirmed the close relationship postulated betweenExophiala dermatitidis, Wangiella dermatitidis andSarcinomyces phaeomuriformis. This monophyletic clade also contains the teleomorph speciesCapronia mansonii thus confirming the concept of a teleomorph connection of the genusExophiala to a member of the Herpotrichiellaceae. However,Exophiala castellanii did not belong to this clade. Therefore, this species is not the anamorph ofCapronia mansonii as it was postulated. 相似文献
8.
The Antwerp database on small ribosomal subunit RNA now offers more than 6000 nucleotide sequences (August 1996). All these sequences are stored in the form of an alignment based on the adopted secondary structure model, which is corroborated by the observation of compensating substitutions in the alignment. Besides the primary and secondary structure information, literature references, accession numbers and detailed taxonomic information are also compiled. For ease of use, the complete database is made available to the scientific community via World Wide Web at URL http://rrna.uia.ac.be/ssu/ . 相似文献
9.
The Antwerp database on small ribosomal subunit RNA offers over 4300 nucleotide sequences (August 1995). All these sequences are stored in the form of an alignment based on the adopted secondary structure model, which in turn is corroborated by the observation of compensating substitutions in the alignment. Besides the primary and secondary structure information, literature references, accession numbers and detailed taxonomic information are also compiled. The complete database is made available to the scientific community through anonymous ftp and World Wide Web(WWW). 相似文献
10.
About 8600 complete or nearly complete sequences are now available from the Antwerp database on small ribosomal subunit RNA. All these sequences are aligned with one another on the basis of the adopted secondary structure model, which is corroborated by the observation of compensating substitutions in the alignment. Literature references, accession numbers and detailed taxonomic information are also compiled. The database can be consulted via the World Wide Web at URL http://rrna.uia.ac.be/ssu/ 相似文献
11.
Y Van de Peer E Robbrecht S de Hoog A Caers P De Rijk R De Wachter 《Nucleic acids research》1999,27(1):179-183
Over 11 500 complete or nearly complete sequences are now available from the Antwerp database on small subunit ribosomal RNA. All these sequences are aligned with one another on the basis of the adopted secondary structure model, which is corroborated by the observation of compensating substitutions in the alignment. Literature references, accession numbers and taxonomic information are also compiled. The database can be consulted via the World Wide Web at URL http://rrna.uia.ac.be/ssu/ 相似文献
12.
Using nucleotide sequences of 5S ribosomal RNAs from 2 hydrozoan jellyfishes, 3 scyphozoan jellyfishes and 2 sea anemones, a phylogenetic tree of Cnidaria has been constructed to elucidate the evolutionary relationships of radial and bilateral symmetries. The 3 classes of Cnidaria examined herein belong to one branch, which does not include other metazoan phyla such as the Platyhelminthes. The Hydrozoa (having radial symmetry without septa) and the Scyphozoa (having radial symmetry with septa) are more closely related to each other than to the Anthozoa (having bilateral symmetry with septa). In classical taxonomy, multicellular animals are considered to have evolved through organisms with radial symmetry (e.g., Cnidaria) to bilateral symmetry. Our results, however, indicate that the emergence of the Bilateria was earlier than that of the Radiata, suggesting (in opposition to Haeckel's view) that the radial symmetry of Cnidaria is an evolutionary dead end. 相似文献
13.
The database on small ribosomal subunit RNA structure contains (June 1994) 2824 nucleotide sequences. All these sequences are stored in the form of an alignment based on the adopted secondary structure model, which in turn is corroborated by the observation of compensating substitutions in the alignment. The complete database is made available to the scientific community through anonymous ftp on our server in Antwerp. A special effort was made to improve electronic retrieval and a program is supplied that allows to create different file formats. The database can also be obtained from the EMBL nucleotide sequence library. 相似文献
14.
Nucleotide sequences of the small-subunit (SSU) ribosomal DNA were determined forPelvetia babingtonii, P. canaliculate, Pelvetiopsis limitata, andAscophyllum nodosum in the family Fucaceae. A total of 1755 positions were aligned for the whole sequence. The positional differences in the
primary structure among the taxa ranged from 16 to 30 nucleotide changes in pairwise comparisons. There was a minimum divergence
betweenPs. limitata andP. babingtonii while a maximum betweenPs. limitata andP. canaliculata. The SSU rDNA trees showed that the genusPelvetia was not monophyletic and the genusPelvetiopsis was not closely related toPelvetia. Our results suggest that the taxonomic revision of the genusPelvetia as well as the family Fucaceae is needed based on detailed morphological observations. 相似文献
15.
J M Neefs Y Van de Peer P De Rijk S Chapelle R De Wachter 《Nucleic acids research》1993,21(13):3025-3049
The database on small ribosomal subunit RNA structure contained 1804 nucleotide sequences on April 23, 1993. This number comprises 365 eukaryotic, 65 archaeal, 1260 bacterial, 30 plastidial, and 84 mitochondrial sequences. These are stored in the form of an alignment in order to facilitate the use of the database as input for comparative studies on higher-order structure and for reconstruction of phylogenetic trees. The elements of the postulated secondary structure for each molecule are indicated by special symbols. The database is available on-line directly from the authors by ftp and can also be obtained from the EMBL nucleotide sequence library by electronic mail, ftp, and on CD ROM disk. 相似文献
16.
Alternative base pairing between 5''- and 3''-terminal sequences of small subunit RNA may provide the basis of a conformational switch of the small ribosomal subunit. 总被引:4,自引:2,他引:2 下载免费PDF全文
The compiled sequences of small subunit ribosomal RNAs have been screened for base complementary between 5'- and 3'-terminal regions. Highly conserved complementary sequences are found which allow formation of a helix between the two ends of 5 or 6 base pairs. This helix is composed of sequences from the loop region of the first 5'-terminal stem and from sequences immediately distal to the last stem (the Me2A-stem) of the 3' terminus and therefore allows a coaxial stacking with either of these two flanking stems. Formation of the 5'/3'-helical arrangement is, however, only possible at the cost of dissolving the 'pseudo-knot' helix between the 5'-terminal region and the internal region of small subunit RNA. It is postulated that the mutually exclusive conformational states are in dynamic equilibrium and that they correlate with distinct functional states of the small ribosomal subunit. The 'pseudo-knot' containing conformation with the 3'-terminal sequences more exposed is likely to represent the initiating state, whereas the 5'/3' terminal paired 'closed' conformation may represent the elongating state in which interaction with fortuitous ribosomal binding sequences of mRNAs is avoided. 相似文献
17.
Discrimination of Porphyra species based on small subunit ribosomal RNA gene sequence 总被引:2,自引:0,他引:2
M. Kunimoto H. Kito Y. Yamamoto D.P. Cheney Y. Kaminishi Y. Mizukami 《Journal of applied phycology》1999,11(2):203-209
The complete nucleotide sequences of ssu rRNA genes were determined for nine species of Porphyra. Ssu rRNA gene structure
was classified into four types by the presence and absence of intron(s). Gene structure even differed within the same species.
Exon nucleotide sequences were identical within the same species, but differed among species. Seventeen species of Porphyra
were discriminated by comparing the sequences of these diversified regions, using the results of this study and previous studies.
This revised version was published online in July 2006 with corrections to the Cover Date. 相似文献
18.
The phylogeny of the Hyphochytriomycota as deduced from ribosomal RNA sequences of Hyphochytrium catenoides 总被引:1,自引:0,他引:1
Van der Auwera G; De Baere R; Van de Peer Y; De Rijk P; Van den Broeck I; De Wachter R 《Molecular biology and evolution》1995,12(4):671-678
Based on biochemical and ultrastructural data, hyphochytriomycetes are
believed to share an ancestor with oomycetes and heterokont algae. In order
to study the phylogeny of the hyphochytriomycetes, we determined both the
small- and large-subunit ribosomal RNA sequence of Hyphochytrium
catenoides. Phylogenetic trees were constructed using the neighbor-joining
and maximum-parsimony method and include representatives of Chlorobionta,
Fungi, Metazoa, Alveolata, and all known Heterokonta. Our main conclusion
is that the hyphochytriomycetes form a monophyletic group with the
oomycetes and heterokont algae and that they are probably the closest
relatives of the oomycetes. However, the order of divergence between the
various heterokont algal phyla and the oomycete-hyphochytriomycete cluster
remains uncertain.
相似文献
19.
A phylogenetic tree of most of the major groups of organisms has been
constructed from the 352 5S ribosomal RNA sequences now available. The tree
suggests that there are several major groups of eubacteria that diverged
during the early stages of their evolution. Metabacteria (= archaebacteria)
and eukaryotes separated after the emergence of eubacteria. Among
eukaryotes, red algae emerged first; and, later, thraustochytrids (a
Proctista group), ascomycetes (yeast), green plants (green algae and land
plants), "yellow algae" (brown algae, diatoms, and chrysophyte algae),
basidiomycetes (mushrooms and rusts), slime- and water molds, various
protozoans, and animals emerged, approximately in that order. Three major
types of photosynthetic eukaryotes--i.e., red algae (= Chlorophyll a
group), green plants (Chl. a + b group) and yellow algae (Chl. a + c)--are
remotely related to one another. Other photosynthetic unicellular
protozoans--such as Cyanophora (Chl. a), Euglenophyta (Chl. a + b),
Cryptophyta (Chl. a + c), and Dinophyta (Chl. a + c)--seem to have
separated shortly after the emergence of the yellow algae.
相似文献
20.
We sequenced small-subunit ribosomal RNA genes (16S-like rDNAs) of 10 species belonging to the genera Entamoeba and Endolimax. This study was undertaken to (1) resolve the relationships among the major lineages of Entamoeba previously identified by riboprinting; (2) examine the validity of grouping the genera Entamoeba and Endolimax in the same family, the Entamoebidae; and (3) examine how different models of nucleotide evolution influence the position of Entamoeba in eukaryotic phylogenetic reconstructions. The results obtained with distance, parsimony, and maximum-likelihood analyses support monophyly of the genus Entamoeba and are largely in accord with riboprinting results. Species of Entamoeba producing cysts with the same number of nuclei from monophyletic groups. The most basal Entamoeba species are those that produce cysts with eight nuclei, while the group producing four-nucleated cysts is most derived. Most phylogenetic reconstructions support monophyly of the Entamoebidae. In maximum-likelihood and parsimony analyses, Endolimax is a sister taxon to Entamoeba, while in some distance analyses, it represents a separate lineage. The secondary loss of mitochondria and other organelles from these genera is confirmed by their relatively late divergence in eukaryotic 16S-like rDNA phylogenies. Finally, we show that the positions of some (fast-evolving) eukaryotic lineages are uncertain in trees constructed with models that make corrections for among-site rate variation. 相似文献