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1.
Juvenile hormone III was identified in whole-body extracts of larval and adult Aedes aegypti. No juvenile hormone I or II was detected. The activity of juvenile hormones I, II and III, as well as two juvenile hormone analogues (methoprene, or ZR-515 and ZR-371) was examined in adults, whereas the activity of only the three naturally occurring hormones was studied in larvae. In the larval assay fourth-instar larvae were exposed to the juvenile hormones and their ability to eclose normally was measured. In the adult assay, abdomens were removed shortly after eclosion and the juvenile hormones or analogues were applied topically. Growth of the oöcytes to the resting stage was measured. In larval and adult bioassays juvenile hormone I was 10 × and 25 × more active, respectively, than juvenile hormone III. The bioassay and titre data taken together suggest that juvenile hormone III is the sole physiologically necessary juvenile hormone in Aedes.  相似文献   

2.
Alpha-ecdysone advances the metamorphosis of female sexual larvae in the last instar and reduces the size of adults that emerge. These adults are either small queens or intercastes or workers, instead of large queens. Treatment, after adult buds have begun to differentiate, gives a greater effect than earlier treatment, though such early treatment probably reduces resistance to ecdysone later on. The juvenile hormone analogues, farnesenic acid and farnesenyl-methyl-ether, applied topically and injected in olive oil, delay metamorphosis and permit female sexual larvae to grow bigger and generate more as well as larger queens. Post-cephalic ligature which excludes both the cerebral ganglia and the corpora allata enables the leg buds to grow and segment. As elimination of the cerebral ganglia by extrusion through the cuticle does not release leg segmentation it is presumed that the corpora allata are secreting a development inhibitor. In confirmation, juvenile hormone analogues applied topically to ligatured larvae inhibit leg development. Morphometric evidence suggests that an apolysis has been suppressed at the end of larval life; this has left the endocrine mechanism free to evolve into a system for the generation of two distinct morphs.  相似文献   

3.
The Methoprene-tolerant (Met) gene of Drosophila melanogaster is involved in both juvenile hormone (JH) action and resistance to JH insecticides, such as methoprene. Although the consequences of Met mutations on development and methoprene resistance are known, no studies have examined Met+ overexpression. Met+ was overexpressed in transgenic lines with various promoters that drive overexpression to different levels. Flies expressing either genomic or cDNA Met+ transgenes showed higher susceptibility to both the morphogenetic and toxic effects of methoprene, consistent with the hormone-binding property of MET. Both the sensitive period and lethal period were the same as seen for non-overexpressing Met+ flies. However, continual exposure of high-overexpressing Met+ larvae to borderline-toxic or higher methoprene doses advanced the sensitive period from prepupae to first instar and the lethal period from pharate adults to larvae and early pupae. When expression of transgenic UAS-Met+ was driven to high levels by either an actin-GAL4 or tubulin-GAL4 promoter, larvae showed high mortality in the absence of methoprene, indicating that high MET titer is lethal, perhaps resulting from expression in an inappropriate tissue. Adults overexpressing Met+ did not show enhanced oogenesis, ruling out MET as a limiting factor for this hormone-driven physiology.  相似文献   

4.
Tyrosine glucoside (α-d-glucopyranosyl-O-l-tyrosine) serves as a reservoir of tyrosine and glucose for pupal and adult cuticle formation, tanning, and pigmentation in several Lepidopteran insects. In the tobacco hornworm, Manduca sexta (L.), detectable quantities appear in the haemolymph 1–2 days after ecdysis of the fifth instar and very high concentrations accumulate between the fourth and eighth days of the stadium. If juvenile hormone II or a mimic (methoprene) is injected into fifth-instar larvae at 24-h intervals after ecdysis, tyrosine glucoside synthesis is almost completely suppressed. Temporary starvation of newly ecdysed larvae that results in the maintenance of a high endogenous juvenile hormone titre, also suppresses and delays the onset of tyrosine glucoside synthesis. The decrease and eventual disappearance of juvenile hormone after ecdysis of the last-larval instar appears to be a necessary prerequisite for the synthesis or activation of tyrosine glucoside synthetase along with the initiation of other metamorphic events.  相似文献   

5.
The Methoprene-tolerant (Met) mutation of Drosophila melanogaster results in a high (100-fold) level of resistance to the insecticide methoprene, a chemical analog of juvenile hormone. Pest species that are under control with methoprene may therefore have the potential to evolve resistance via a mutation homologous to Met. To evaluate the potential of such mutants to persist in wild populations, we must understand the fitness of flies carrying Met. In the absence of methoprene, Met flies were outcompeted by a wild-type strain both in a multigeneration population cage and in single-generation competition experiments. To determine which fitness component(s) is responsible for the competitive disadvantage, the survival, time of development, and fecundity of flies homozygous for each of five Met alleles were compared with wild type. Small but significant differences were found between the pooled Met alleles and wild type for pupal development time, pupal mortality, and early adult fecundity. These differences result in a large competitive disadvantage. Although Met flies were found to have reduced fitness by these measures, the phenotype is not as severe as might be expected from a knowledge of the disruption of juvenile hormone regulation seen in Met flies. It is concluded that (1) although Met flies have a large advantage under methoprene selection, they will quickly become outcompeted upon relaxation of methoprene usage, (2) even a seemingly severe disruption of juvenile hormone regulation has no drastic effect on the vital functions of the insect and (3) small differences in fitness components can translate into a large competitive disadvantage.  相似文献   

6.
7.
The larval fat body of newly eclosed adults of Drosophila melanogaster was found to contain a single major binding protein specific for juvenile hormone (JH). Binding to this protein was saturable, of high affinity, and specific for JH III. The protein has a subunit molecular weight (Mr) of 85,000, as determined by photoaffinity labeling. The same or similar JH-binding protein was found in larval fat body and cuticle of third instar larvae and in male accessory glands and heads of newly eclosed adults. It was not found in several other tissues in adults. Male accessory gland cytosol from wild-type flies was found to contain a single binder with a dissociation constant (KD) of 6.7 nM for JH III; a binder in similar preparations from the methoprene-tolerant (Met) mutant had a KD value 6-fold higher. JH III stimulated protein synthesis in glands cultured in vitro, but this effect was reduced in Met flies as compared to wild-type flies, establishing a correlation between JH binding and biological activity of the hormone. In addition, glandular protein accumulation during the first 2 days of adult development was less in Met flies than in wild-type flies. These results strongly suggest that the binding protein we have identified mediates this JH effect in male accessory glands and thus is acting as a JH receptor.  相似文献   

8.
Konopova B  Smykal V  Jindra M 《PloS one》2011,6(12):e28728
Insect larvae metamorphose to winged and reproductive adults either directly (hemimetaboly) or through an intermediary pupal stage (holometaboly). In either case juvenile hormone (JH) prevents metamorphosis until a larva has attained an appropriate phase of development. In holometabolous insects, JH acts through its putative receptor Methoprene-tolerant (Met) to regulate Krüppel-homolog 1 (Kr-h1) and Broad-Complex (BR-C) genes. While Met and Kr-h1 prevent precocious metamorphosis in pre-final larval instars, BR-C specifies the pupal stage. How JH signaling operates in hemimetabolous insects is poorly understood. Here, we compare the function of Met, Kr-h1 and BR-C genes in the two types of insects. Using systemic RNAi in the hemimetabolous true bug, Pyrrhocoris apterus, we show that Met conveys the JH signal to prevent premature metamorphosis by maintaining high expression of Kr-h1. Knockdown of either Met or Kr-h1 (but not of BR-C) in penultimate-instar Pyrrhocoris larvae causes precocious development of adult color pattern, wings and genitalia. A natural fall of Kr-h1 expression in the last larval instar normally permits adult development, and treatment with an exogenous JH mimic methoprene at this time requires both Met and Kr-h1 to block the adult program and induce an extra larval instar. Met and Kr-h1 therefore serve as JH-dependent repressors of deleterious precocious metamorphic changes in both hemimetabolous and holometabolous juveniles, whereas BR-C has been recruited for a new role in specifying the holometabolous pupa. These results show that despite considerable evolutionary distance, insects with diverse developmental strategies employ a common-core JH signaling pathway to commit to adult morphogenesis.  相似文献   

9.
Pupae of Hyalophora cecropia were injected with various doses of beta-ecdysone (molting hormone) or juvenile hormone and the epidermal cell ultrastructure was then studied with the electron microscope. The hormonal effects were rapidly manifested and appeared to be cell specific and dose dependent. The initial response to both hormones was an outward blebbing of the apical plasma membrane. Large doses of beta-ecdysone elicited both precocious cuticle deposition and premature autophagic vacuole formation. Juvenile hormone prevented the appearance of the autophagic vacuoles which normally preceded cell differentation into cells capable of adult synthesis. After prolonged exposure to juvenile hormone, there appeared to be an exaggerated separation of the epidermal cells at the basal region suggesting that the juvenile hormone may act at the membrane level.  相似文献   

10.
11.
Wing discs from late final-instar Ephestia larvae form only pupal cuticle when immediately implanted into pupae which subsequently undergo metamorphosis. However, either pupal or adult structures are made in vitro depending on (1) the ecdysterone dose and/or (2) disc cell proliferation. Continuous culture in ecdysterone (0.5–5.0 μg/ml) results in the appearance of transparent cuticle. On the basis of several criteria, this untanned cuticle is postulated to be scaleless adult cuticle. Discs pulsed with 0.5 μg/ml ecdysterone for 48–120 hr, or with 5.0 μg/ml for 24 hr, formed tanned cuticle. Lower doses of ecdysterone (i.e., 0.5 μg/ml for 24 hr or continuous exposure to 0.05 μg/ml) trigger adult scale formation. Enhancement of [3H]thymidine incorporation by these latter doses suggests the occurrence of disc cell divisions and polyploidization. The choice between pupal and adult pathways by wing discs of this age can be controlled exclusively by ecdysterone; juvenile hormone need not be involved in vitro.  相似文献   

12.
Treatment of post-feeding (early day 3; wandering phase) last-stadium larvae of the cabbage looper, Trichoplusia ni, with the anti-juvenile hormone, fluoromevalonolactone, prevented the normal ecdysis to the pupa. It caused the formation of larval-pupal intermediates, a dose-dependent delay in the time of tanning, and a decrease in juvenile hormone esterase activity at the time of the prepupal juvenile hormone esterase peak. Fluoromevalonolactone was inactive as juvenile hormone esterase inhibitor in vitro. Conversely, juvenile hormone I accelerated the time of tanning, induced the early appearance of juvenile hormone esterase activity, and prevented adult eclosion. Although most of the larvae that were treated with fluoromevalonolactone immediately after the prepupal burst of juvenile hormone (late on day 3; post-spinning phase) still became larval-pupal intermediates, the time of tanning and juvenile hormone esterase activity were close to normal. Topical treatment of day-3 larvae with radiolabelled juvenile hormone I resulted in the rapid appearance and decline of radiolabelled juvenile hormone I in the haemolymph which was associated with the increased production of juvenile hormone I acid and the induced appearance of juvenile hormone esterase activity. Thus, in post-feeding last-stadium larvae of T. ni, juvenile hormone seems to be necessary for the proper formation of the pupa. Juvenile hormone is also involved in determining the time of pupation, and it appears to induce its own degradation.  相似文献   

13.
The regulation of juvenile hormone esterase in last-instar diapause and nondiapause larvae of Ostrinia nubilalis was investigated using topically applied juvenile hormone I and a juvenile hormone mimic, methoprene. The influence of the head on juvenile hormone esterase was also investigated. Both juvenile hormone and methoprene caused increases in esterase levels when applied to feeding animals. Neither the hormone nor methoprene was capable of elevating nondiapause esterase activity to levels comparable to those found in untreated prediapause larvae. The esterase levels could be elevated in the larval body, without the head, during prepupal development of nondiapause larvae and in post-feeding diapause larvae. In both cases, juvenile hormone or methoprene induced juvenile hormone esterase activity in head-ligated animals. Topically applied methoprene prolonged feeding and delayed the onset of diapause. When methoprene was applied to larvae that had entered diapause, it disrupted diapause by inducing a moult.  相似文献   

14.
The morphogenetic action of several structurally unrelated bioanalogues of juvenile hormone resulted in two different types of effects: (1) Topical application of these compounds on worker bee larvae prior to the cessation of feeding induced development of imaginal structures characteristic for queen individuals and (2) administration of the compounds at later stages caused inhibition of differentiation of imaginal characters at various phases of adult development. The moment of application appeared to be crucial for the extent and quality of these effects. Effects on pupal morphogenesis were not proved unequivocally, since the formation of a second pupal cuticle was not observed. The juvenile hormone activities of 33 chemicals representing several types of hormonally active agents have been compared and some principal structure-activity relationships have been outlined.  相似文献   

15.
【目的】黑化反应在昆虫表皮骨化以及免疫防御过程中起着重要作用, 酚氧化酶是黑化反应中的关键酶类, 漆酶2 (laccase2, LAC2)是酚氧化酶的一种, 在昆虫变态发育和免疫系统中起着重要的作用。本研究旨在探讨LAC2在棉铃虫Helicoverpa armigera表皮骨化中表达模式及激素调控作用。 【方法】采用PCR及RACE的方法, 从棉铃虫5龄幼虫中得到了lac2 cDNA全序列。利用荧光定量PCR、 激素处理及RNA干扰方法, 对LAC2的表达模式差异和激素调控作用进行分析。【结果】序列分析表明, lac2 cDNA全长3 221 bp, 编码框长度为2 268 bp, 编码756个氨基酸残基。发育时序表达分析发现, lac2在幼虫各龄期表达规律相似, 均在蜕皮期高水平表达, 在5龄96 h转录水平达到最高峰。组织表达结果分析, lac2基因在幼虫表皮和成虫卵巢以及触角表达量较高。激素处理实验发现, 保幼激素类似物(methoprene)对lac2基因转录有抑制作用; 蜕皮激素(20-hydroxyecdysone)则促进其表达。进一步利用RNA干扰蜕皮激素受体EcR (ecdysone receptor)和USP (ultraspiracle isoform)基因发现, 干扰后蜕皮激素受体的表达明显受到抑制, 同时lac2基因的表达也显著受到抑制, 表明蜕皮激素调控lac2基因转录。【结论】这些结果为进一步研究漆酶在昆虫表皮的骨化以及免疫防御等方面不同的生理功能提供理论依据。  相似文献   

16.
The Methoprene-tolerant (Met) bHLH-PAS gene is involved in juvenile hormone (JH) action in Drosophila melanogaster as a likely component of a JH receptor. We expressed Met in Drosophila S2 cells and explored for MET partners using pull-down assays. MET-MET interaction was found to occur. The germ-cell expressed (gce) gene is another D. melanogaster bHLH-PAS gene with high homology to Met, and GCE formed heterodimers with MET. In the presence of JH or either of two JH agonists, MET-MET and MET-GCE formation was drastically reduced. Interaction between GCE and MET having N- or C-terminus truncations, bHLH or PAS-A domain deletions, or a point mutation in the PAS-B domain failed to occur. However, JH-dependent interaction occurred between GCE and MET having point mutations in bHLH or PAS-A. During development, changes in JH titer may alter partner binding by MET and result in different gene expression patterns.  相似文献   

17.
A method was developed to determine in the same extract juvenile hormone and various types of ecdysteroids in precisely staged eggs and larvae of Trichoplusia ni. Ecdysteroids were tentatively identified on the basis of their retention time in ion suppression reversed-phase HPLC and their cross-reactivity with two relatively non-specific, complimentary antibodies, whereas juvenile hormone was identified using reversed-phase HPLC combined with Galleria bioassay. Freshly laid eggs contained low levels of immunoreactive ecdysteroids. Mid-polar ecdysteroids increased in the phase of segmentation (14-18 h) and 1st larval cuticle formation (36-44 h), when 20-hydroxyecdysone and 20,26-dihydroxyecdysone were found to be predominant. Only traces of ecdysone and 26-hydroxyecdysone were seen. Toward hatching ecdysteroids decreased and represented mainly compounds more polar than 20,26-dihydroxyecdysone. In larval development ecdysteroids were low at the beginning of the feeding phases, increased toward cessation of feeding, and reached highest levels 12-15 h before ecdysis. In feeding stages ecdysone and 20-hydroxyecdysone were predominant, whereas in molting stages they were seen together with 20,26-dihydroxyecdysone and 20-hydroxyecdysonoic acid. The juvenile hormone titer was very low in freshly laid eggs and was high (approximately 25 ng/g) in embryos at the stage of 1st larval cuticle formation and eye pigmentation. In eggs we tentatively identified juvenile hormones I and II, whereas in larval stages juvenile hormone II appeared to be the predominant or exclusive juvenile hormone. Its titer fluctuated rapidly and was high in early 1st-instar larvae and again before the molts into the 3rd, 4th, and 5th instar. Highest titers were reached concomitant with the peak in 20-hydroxyecdysone 12-15 h before ecdysis.  相似文献   

18.
《Insect Biochemistry》1990,20(5):451-459
Juvenile hormone esterase, purified by affinity chromatography from the larval hemolymph of Manduca sexta in the fifth stadium, was injected into larvae of the same species in the earlier stadia resulting in a blackening of the cuticle following ecdysis to the next larval stadium. This anti-juvenile hormone response was dose-dependent for an injection in the second, third or fourth stadium. Cuticular blackening was prevented by treating larvae with the juvenoid epofenonane. Larval response to injected juvenile hormone esterase also varied with the time of injection within a single stadium, having a maximum effect for injections at the time of head capsule slippage. Juvenile hormone esterase activity measured from the hemolymph after injection of larvae in the second stadium decreased over an 11 h time-course. Because the anti-juvenile hormone effects resulting from a single injection of juvenile hormone esterase were dependent on the time of injection, it appears that when juvenile hormone biosynthesis is active in the insect, the duration of enzyme activity limits the anti-juvenile effects that can be induced.  相似文献   

19.
The understanding of the molecular basis of the endocrine control of insect metamorphosis has been hampered by the profound differences in responses of the Lepidoptera and the Diptera to juvenile hormone (JH). In both Manduca and Drosophila, the broad (br) gene is expressed in the epidermis during the formation of the pupa, but not during adult differentiation. Misexpression of BR-Z1 during either a larval or an adult molt of Drosophila suppressed stage-specific cuticle genes and activated pupal cuticle genes, showing that br is a major specifier of the pupal stage. Treatment with a JH mimic at the onset of the adult molt causes br re-expression and the formation of a second pupal cuticle in Manduca, but only in the abdomen of DROSOPHILA: Expression of the BR isoforms during adult development of Drosophila suppressed bristle and hair formation when induced early or redirected cuticle production toward the pupal program when induced late. Expression of BR-Z1 at both of these times mimicked the effect of JH application but, unlike JH, it caused production of a new pupal cuticle on the head and thorax as well as on the abdomen. Consequently, the 'status quo' action of JH on the pupal-adult transformation is mediated by the JH-induced re-expression of BR.  相似文献   

20.
When larval tissue is exposed to a hormonal milieu lacking juvenile hormone, adult characters appear directly, omitting the pupal stage, in some insects but not in others, including Bombyx mori. An attempt was made to induce omission of pupal characters in this species by varying the stage of the larval epidermis to be tested. Pieces of larval integument taken from fourth- and fifth-instar larvae of various stages were transplanted to developing adults. Although the number of cuticle layers and the types of cuticle produced differed depending on the age of the donors, none of the pieces omitted secreting the pupal cuticle. It is concluded that the larval epidermis cannot omit secreting pupal cuticle, and that a transition of tissue competence may play an important part in the sequential appearance of larval, pupal, and adult characters.  相似文献   

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