Carbon impacts of direct land use change in semiarid woodlands converted to biofuel plantations in India and Brazil

We present an analysis of direct land use change (dLUC) resulting from the conversion of semiarid woodlands in Brazil and India to Jatropha curcas, a perennial biofuel crop. The sites examined include prosopis woodlands, managed for woodfuel production under periodic coppicing, in southern India, and unmanaged caatinga woodlands in the Brazilian state of Minas Gerais. The jatropha plantations under consideration include pruned and unpruned stands and ranged from 2 to 4 years of age. Stocks of carbon in aboveground (AG) pools, including woody biomass, coarse debris, leaf litter, and herbaceous matter, as well as soil organic carbon (SOC) were evaluated. The jatropha plantations store 8–10 tons of carbon per hectare (t C ha^?1) in AG biomass and litter when managed with regular pruning in both India and Brazil. Unpruned trees, only examined in Brazil, store less biomass (and carbon), accumulating just 3 t C ha^?1 in AG pools. The two woodlands that were replaced with jatropha show substantial differences in carbon pools: prosopis contains ～11 t C ha^?1 in AG stocks of carbon, which was very close to the jatropha stand which replaced it. In contrast, caatinga stores ～35 t C ha^?1 in AG biomass. Moreover, no change in SOC was detected in land that was converted from Prosopis to jatropha. As a result, there is no detectable change in AG carbon stocks at the sites in South India where jatropha replaced prosopis woodlands. In contrast, large losses of AG carbon were detected in Central Brazil where jatropha replaced native caatinga woodlands. These losses represent a carbon debt that would take 10–20 years to repay.     

Regional water resource implications of bioethanol production in the Southeastern United States

The Energy Independence and Security Act (EISA) of 2007 mandates US production of 136 billion L of biofuel by 2022. This target implies an appropriation of regional primary production for dedicated feedstocks at scales that may dramatically affect water supply, exacerbate existing water quality challenges, and force undesirable environmental resource trade offs. Using a comparative life cycle approach, we assess energy balances and water resource implications for four dedicated ethanol feedstocks – corn, sugarcane, sweet sorghum, and southern pine – in two southeastern states, Florida and Georgia, which are a presumed epicenter for future biofuel production. Net energy benefit ratios for ethanol and coproducts range were 1.26 for corn, 1.94 for sweet sorghum, 2.51 for sugarcane, and 2.97 for southern pine. Corn also has high nitrogen (N) and water demand (11.2 kg GJ_net^?1 and 188 m³ GJ_net^?1, respectively) compared with other feedstocks, making it a poor choice for regional ethanol production. Southern pine, in contrast, has relatively low N demand (0.4 kg GJ_net^?1) and negligible irrigation needs. However, it has comparatively low gross productivity, which results in large land area per unit ethanol production (208 m² GJ_net^?1), and, by association, substantial indirect and incremental water use (51 m³ GJ_net^?1). Ultimately, all four feedstocks require substantial land (10.1, 3.1, 2.5, and 6.1 million ha for corn, sugarcane, sweet sorghum, and pine, respectively), annual N fertilization (3230, 574, 396, 109 million kg N) and annual total water (54 400, 20 840, 8840, and 14 970 million m³) resources when scaled up to meet EISA renewable fuel standards production goals. This production would, in turn, offset only 17.5% of regional gasoline consumption on a gross basis, and substantially less when evaluated on a net basis. Utilization of existing waste biomass sources may ameliorate these effects, but does not obviate the need for dedicated primary feedstock production. Careful scrutiny of environmental trade‐offs is necessary before embracing aggressive ethanol production mandates.     

Durability of rubberwood (Hevea brasiliensis) treated with peppermint oil,eucalyptus oil,and their main components

Anti-fungal activities of two essential oils (peppermint oil and eucalyptus oil) and their main components (menthol and eucalyptol, respectively) against molds (Aspergillus niger, Penicillium chrysogenum, and Penicillium sp.) and a white-rot decay fungus (Trametes versicolor) identified from rubberwood surfaces were investigated. The broth dilution method and the agar diffusion technique were employed to determine the minimal inhibitory concentration (MIC) and the minimal fungicidal concentration (MFC) using the concentration of substances between 100 and 800 μl ml^?1. Inhibitory effects of essential oils and their main components at the MICs against mold growth, fungal decay, and termite attack on rubberwood were further examined by means of the dip treatment method. It was found that MFC values against molds for all treatments examined were about 50–100 μl ml^?1 higher than MIC values. Peppermint oil and menthol exhibited high fungistatic and fungicidal activities, with MICs of 300 μl ml^?1 and 350 μl ml^?1, respectively, against the test molds and the decay fungus. Eucalyptus oil and eucalyptol were also effective against these microbes but at higher concentrations of 600 μl ml^?1 and 500 μl ml^?1, respectively. Only peppermint oil at the MIC was capable of providing a complete protection from mold growth on rubberwood for up to 12 weeks at storage conditions of 25 °C and 100% RH. Both peppermint oil and eucalyptus oil at the MICs showed moderate resistance to fungal decay and high resistance to termite attack.     

Enhanced enzyme production with the pelleted form of <Emphasis Type="Italic">D. squalens</Emphasis> in laboratory bioreactors using added natural lignin inducer

White-rot fungi are extensively used in various submerged biotechnology processes to produce ligninolytic enzymes. Transfer of the process from the laboratory to the industrial level requires optimization of the cultivation conditions on the laboratory scale. An interesting area of optimization is pellet growth since this morphological form solves problems such as the decreased oxygen concentration, limited heat, and nutrient transport, which usually occur in dispersed mycelium cultures. Many submerged fermentations with basidiomycetes in pellet form were done with Phanerochaete, Trametes, and Bjerkandera species, among others. In our study, another promising basidiomycete, D. squalens, was used for ligninolytic enzyme production. With the addition of wood particles (sawdust) as a natural inducer and optimization of mixing and aeration conditions in laboratory stirred tank (STR) and bubble column (BCR) reactors on pellet growth and morphology, the secretion of laccase and the manganese-dependent peroxidase into the medium was substantially enhanced. The maximum mean pellet radius was achieved after 10 days in the BCR (5.1 mm) where pellets were fluffy and 5 days in the STR (3.5 mm) where they were round and smooth. The maximum Lac activity (1,882 U l⁻¹) was obtained after 12 days in the STR, while maximum MnP activity (449.8 U l⁻¹) occurred after 18 days in the BCR. The pellet size and morphology depended on the agitation and aeration conditions and consequently influenced a particular enzyme synthesis. The enzyme activities were high and comparable with the activities found for other investigations in reactors with basidiomycetes in the form of pellets.     

Projections of the availability and cost of residues from agriculture and forestry

By‐products of agricultural and forestry processes, known as residues, may act as a primary source of renewable energy. Studies assessing the availability of this resource offer little insight on the drivers and constraints of the available potential as well as the associated costs and how these may vary across scenarios. This study projects long‐term global supply curves of the available potential using consistent scenarios of agriculture and forestry production, livestock production and fuel use from the spatially explicit integrated assessment model IMAGE. In the projections, residue production is related to agricultural and forestry production and intensification, and the limiting effect of ecological and alternative uses of residues are accounted for. Depending on the scenario, theoretical potential is projected to increase from approximately 120 EJ yr^?1 today to 140–170 EJ yr^?1 by 2100, coming mostly from agricultural production. To maintain ecological functions approximately 40% is required to remain in the field, and a further 20–30% is diverted towards alternative uses. Of the remaining potential (approximately 50 EJ yr^?1 in 2100), more than 90% is available at costs <10By‐products of agricultural and forestry processes, known as residues, may act as a primary source of renewable energy. Studies assessing the availability of this resource offer little insight on the drivers and constraints of the available potential as well as the associated costs and how these may vary across scenarios. This study projects long‐term global supply curves of the available potential using consistent scenarios of agriculture and forestry production, livestock production and fuel use from the spatially explicit integrated assessment model IMAGE. In the projections, residue production is related to agricultural and forestry production and intensification, and the limiting effect of ecological and alternative uses of residues are accounted for. Depending on the scenario, theoretical potential is projected to increase from approximately 120 EJ yr^?1 today to 140–170 EJ yr^?1 by 2100, coming mostly from agricultural production. To maintain ecological functions approximately 40% is required to remain in the field, and a further 20–30% is diverted towards alternative uses. Of the remaining potential (approximately 50 EJ yr^?1 in 2100), more than 90% is available at costs <10$₂₀₀₅ GJ^?1. Crop yield improvements increase residue productivity, albeit at a lower rate. The consequent decrease in agricultural land results in a lower requirement of residues for erosion control. The theoretical potential is most sensitive to baseline projections of agriculture and forestry demand; however, this does not necessarily affect the available potential which is relatively constant across scenarios. The most important limiting factors are the alternative uses. Asia and North America account for two‐thirds of the available potential due to the production of crops with high residue yields and socioeconomic conditions which limit alternative uses.     

Improving the Profitability of Willow Crops��Identifying Opportunities with a Crop Budget Model

Short-rotation woody crops like shrub willow are a potential source of biomass for energy generation and bioproducts. However, since willow crops are not widely grown in North America, the economics of this crop and the impacts of key crop production and management components are not well understood. We developed a budget model, EcoWillow v1.4 (Beta), that allows users to analyze the entire production-chain for willow systems from the establishment to the delivery of wood chips to the end-user. EcoWillow was used to analyze how yield, crop management options, land rent, fuel, labor, and other costs influence the Internal Rate of Return (IRR) of willow crop systems in upstate New York. We further identified cost variables with the greatest potential for reducing production and transport costs of willow biomass. Productivity of 12 oven-dried tons (odt) ha^?1 year^?1 and a biomass price of $ (US dollars) 60 odt^?1 results in an IRR of 5.5%. Establishment, harvesting, and transportation operations account for 71% of total costs. Increases in willow yield, rotation length, and truck capacity as well as a reduction in harvester down time, land costs, planting material costs, and planting densities can improve the profitability of the system. Results indicate that planting speed and fuel and labor costs have a minimal effect on the profitability of willow biomass crops. To improve profitability, efforts should concentrate on (1) reducing planting stock costs, (2) increasing yields, (3) optimizing harvesting operations, and (4) co-development of plantation designs with new high-yielding clones to reduce planting density.     

Profitability of Willow Biomass Crops Affected by Incentive Programs

The economics of willow biomass crops are strongly influenced by yield, production, and harvesting costs and the delivered price for biomass. Under current management practices, willow biomass crops with yields of 12 oven-dried metric tons (odt)?ha^?1 year^?1 and a delivered price of $60 odt^?1 have an internal rate of return (IRR) of about 5.5 %. Yields below 9 odt ha^?1 year^?1 have an IRR <0 %. We examined the impact of different incentive programs on the returns from willow biomass crops and the hectares or tons of willow biomass supported across a range of yields. Incentive programs examined included establishment grants (EG), annual payments (AIP), low cost startup loans, and matching payments offered by two existing programs, the Conservation Resource Program (CRP) and more recently the Biomass Crop Assistance Program (BCAP). EGs covering 75 % of the establishment costs provide high returns for growers on medium to high-productivity sites. Stand-alone AIPs with payments of $124 ha^?1 year^?1 paid over 5–15 years had little impact on profitability for growers but were costly for a funding agency. Low-cost loans with an interest rate of 2–4 % are one of the least expensive approaches ($1.3–6.6 odt^?1) and improve profitability for medium- and high-yielding (8–16 odt ha^?1 year^?1) sites. A matching payment incentive providing $50 per odt delivered was the only individual incentive approach that made low-yielding sites (6 odt ha^?1 year^?1) profitable but was costly per odt compared to other incentives. Current CRP incentives made willow profitable across all productivity scenarios. The BCAP program generates higher profits for all productivity scenarios but comes at a higher cost. Effective financial incentives need to be well designed and monitored so that the target audience is reached and the intended policy goals are attained.     

Light capture and pigment diversity in marine and freshwater cryptophytes

Phenotypic traits associated with light capture and phylogenetic relationships were characterized in 34 strains of diversely pigmented marine and freshwater cryptophytes. Nuclear SSU and partial LSU rDNA sequence data from 33 of these strains plus an additional 66 strains produced a concatenated rooted maximum likelihood tree that classified the strains into 7 distinct clades. Molecular and phenotypic data together support: (i) the reclassification of Cryptomonas irregularis NIES 698 to the genus Rhodomonas, (ii) revision of phycobiliprotein (PBP) diversity within the genus Hemiselmis to include cryptophyte phycocyanin (Cr‐PC) 569, (iii) the inclusion of previously unidentified strain CCMP 2293 into the genus Falcomonas, even though it contains cryptophyte phycoerythrin 545 (Cr‐PE 545), and (iv) the inclusion of previously unidentified strain CCMP 3175, which contains Cr‐PE 545, in a clade with PC‐containing Chroomonas species. A discriminant analysis‐based model of group membership correctly predicted 70.6% of the clades using three traits: PBP concentration · cell^?1, the wavelength of PBP maximal absorption, and habitat. Non‐PBP pigments (alloxanthin, chl‐a, chl‐c₂, α‐carotene) did not contribute significantly to group classification, indicating the potential plasticity of these pigments and the evolutionary conservation of the PBPs. Pigment data showed evidence of trade‐offs in investments in PBPs vs. chlorophylls (a +c₂).     

Determinants of the economic viability of mallee eucalypts as a short rotation coppice crop integrated into farming systems of Western Australia

Mallee eucalypts are being developed as a short rotation coppice crop for integration into agricultural systems in the south‐west of Western Australia. These have potential for biomass production for bioenergy, eucalyptus oil and generating carbon credits and to help control the extensive occurrence of dryland salinity. Some 12,000 ha of mallee planting has been undertaken since 1994, mostly in the form of wide‐spaced, narrow belts within the annual agricultural system. Production and market data were used to estimate levelized costs (LC) of mallee biomass production under different harvest regimes across 11 sites from 2006 to 2012. We found LC ranged from AUD40 to AUD257 fresh Mg^?1. LC was most strongly determined by mallee production, followed by the crop/pasture rotation decisions of the landholder. Mallee harvest regime had minor impact on LC. Crop and pasture yield loss due to competition from the mallee belts accounted for 38% of costs, harvesting biomass was 32%, opportunity cost of the land occupied by the mallee belts was 16% while establishment and maintenance costs accounted for 14% of the costs. When income from carbon sequestered in mallee root biomass was included, the LC dropped by an average of 11% at the current Australian price of AUD15 Mg^?1 CO₂ equivalent (CO₂e). The income from carbon sequestered in root biomass alone is unlikely to make mallee agroforestry economically viable. Hence, income from harvested biomass in the form of feedstocks for industry or carbon credits is necessary to make mallee agroforestry commercially attractive. LC for unharvested mallee belts ranged from AUD33 to AUD237 Mg^?1. Where above‐ and below‐ground biomass is converted to CO₂e at AUD15 Mg^?1, the LC drops to AUD11–AUD64, with three of 11 sites likely to be profitable. These three sites were characterized by high biomass production with low agricultural gross margins.     

Economic and environmental performance of miscanthus cultivated on marginal land for biogas production

Environmental issues surrounding conventional annual biogas crops have led to growing interest in alternative crops, such as miscanthus. In addition to the better environmental performance, miscanthus can be grown on marginal land where no competition with feed and food crops is anticipated. On marginal land however, biomass yields are significantly lower than on good agricultural land. This raises the question of the economic and environmental sustainability of miscanthus cultivated on marginal land for biogas production. This study assessed the environmental and economic performance of miscanthus cultivated on marginal land for biogas production by conducting a Life‐Cycle Assessment and complementary Life‐Cycle Cost analysis. The functional unit chosen was 1 GJ of electricity (GJ_el.). The substitution of a fossil reference was included using a system expansion approach. Electricity generated by the combustion of miscanthus‐based biogas in a combined heat and power has considerably lower impacts on the environment than the fossil reference in most of the categories assessed. In the impact category “climate change”, the substitution of the marginal German electricity mix leads to a carbon mitigation potential of 256 kg CO₂e/GJ_el.. At 45.12 €/GJ_el., the costs of miscanthus‐based biogas generation and utilization are considerably lower than those of maize (61.30 €/GJ_el.). The results of this study clearly show that it can make economic and environmental sense to cultivate miscanthus on marginal land as a substrate for biogas production. The economic sustainability is however limited by the biomass yield. By contrast, there are no clear thresholds limiting the environmental performance. The decision needs to be made on a case‐by‐case basis depending on site‐specific conditions such as local biodiversity.     

Effects of light intensity and quality on phycobiliprotein accumulation in the cyanobacterium <Emphasis Type="Italic">Nostoc sphaeroides</Emphasis> Kützing

Objectives

To assess the effects of light intensity and quality on the growth and phycobiliproteins (PBP) accumulation in Nostoc sphaeroides Kützing (N. sphaeroides).

Results

Dry weights, dry matter, protein, chlorophyll and PBP contents were higher under 90 μmol m^?2 s^?1 than under other intensities (both higher and lower). Phycocyanin and allophycocyanin increased with light intensity while phycoerythrin decreased. Fresh weights, protein and PBP contents increased at the highest rates under blue light. Red light resulted in higher values of dry matter, phycocyanin and chlorophyll a.

Conclusion

White light at 90 μmol m^?2 s^?1 or blue light 30 μmol m^?2 s^?1 were optimal for the growth and phycobiliprotein accumulation in N. sphaeroides.

     

Biomass production and fatty acid accumulation in Chlorella sp. (strain DEC1B) isolated from a petrol refinery in Huelva (Spain)

A microalgal strain was established from Cepsa's refinery wastewater treatment plant in Huelva (southwest of Spain). Genetic analysis of the chloroplastic rbcL gene encoding for the large subunit of the ribulose bisphosphate carboxylase enzyme (Rubisco) showed the strain had high homology with other known rbcL sequences of the genus Chlorella. The strain grows well autotrophically in minimum mineral medium, with a growth rate of 0.28 ± 0.012 day^?1 and a biomass productivity of 138.9 ± 6.7 mg L^?1 day^?1. N‐starvation and/or over illumination with 650 µmol photons m^?2 s^?1 of PAR light on the cultures induced a significant increase in the intracellular content of lipids in this microalga. Total lipids were extracted from the strain biomass with 2:1 chloroform‐methanol, and they accounted for approximately 50% of the dry biomass. Polyunsaturated fatty acids (PUFAs) represented 60.4% of the total fatty acids found in the strain, thus making this biomass attractive as a high added‐value product source. The strain was able to grow efficiently in the refinery treated wastewater from which it was isolated, providing an attractive advantage for further development of more sustainable algal biomass production processes at reduced costs close to a petrol refinery area.     

Additive interaction of <Emphasis Type="Italic">Helicoverpa armigera</Emphasis> Nucleopolyhedrovirus and Azadirachtin

Mortality of Helicoverpa armigera Hübner (Lepidoptera: Noctuidae) was higher in the combined treatment of Nucleopolyhedrovirus (NPV) and Azadirachtin (AZA) and mortality was increased when AZA concentration was doubled. Larval mortality decreased as the age of the larvae increased in all the treatments. The time for 100% kill of third instar larvae was significantly reduced to 72 h when AZA (0.1 ppm) was combined with NPV (10³ PIB/ml) when compared to 168 and 120 h for the same dose NPV and AZA individual treatments, respectively. The average leaf disc consumption, consumption index (CI), relative growth rate (RGR), the efficiency of conversion of ingested (ECI) and digested (ECD) food values were drastically reduced in the combined treatment of NPV and AZA than in the individual treatments. Larval as well as pupal durations were significantly extended and the adult longevity and fecundity were significantly reduced in the combined treatment of NPV and AZA. Weight of 14 day old control larvae was 420 mg and it was reduced to 299 and 248 mg after NPV (5 × 10² PIB/ml) and AZA (0.025 ppm) treatments, respectively. The larval weight was drastically decreased to 99 mg after the combined treatment at the same dose. The additive interaction between both the treatments, AZA and NPV, was found to be in a dose dependent manner and were highly compatible in disrupting the survival, feeding and biology of H. armigera.     

n-Butanol Production from Acid-Pretreated Jatropha Seed Cake by Clostridium acetobutylicum

n-Butanol fermentation using Clostridium strains suffers from low titers due to the inability of the strains to tolerate n-butanol. The current study demonstrates a process to get high titer of n-butanol in a single batch mode from the renewable feedstock jatropha seed cake by employing Clostridium acetobutylicum. Chemical mutagenesis was done for improvement of the strain for better n-butanol tolerance and production. Optimization of the parameters resulted in 13.2 g L^?1 of n-butanol in 120 h using acid-treated jatropha seed cake hydrolysate (7 %?w/v) in anaerobic sugar medium. The process was scaled up to 15 L level, yielding 18.6 g L^?1 of n-butanol in 72 h. The strain was found to be tolerant up to 30 g L^?1 n-butanol under optimized conditions. The n-butanol tolerance was accompanied by over-expression of the stress response protein, GroEL, change in fatty acid profile, and ability to accumulate rhodamine 6G in the strain. The study has a significant impact on economically producing n-butanol from biomass.     

Production of cyclodextrin glycosyltransferase by immobilized <Emphasis Type="Italic">Bacillus</Emphasis> sp. on chitosan matrix

The whole-cell immobilization on chitosan matrix was evaluated. Bacillus sp., as producer of CGTase, was grown in solid-state and batch cultivation using three types of starches (cassava, potato and cornstarch). Biomass growth and substrate consumption were assessed by flow cytometry and modified phenol–sulfuric acid assays, respectively. Qualitative analysis of CGTase production was determined by colorless area formation on solid culture containing phenolphthalein. Scanning electron microscopy (SEM) analysis demonstrated that bacterial cells were immobilized on chitosan matrix efficiently. Free cells reached very high numbers during batch culture while immobilized cells maintained initial inoculum concentration. The maximum enzyme activity achieved by free cells was 58.15 U ml^?1 (36 h), 47.50 U ml^?1 (36 h) and 68.36 U ml^?1 (36 h) on cassava, potato and cornstarch, respectively. CGTase activities for immobilized cells were 82.15 U ml^?1 (18 h) on cassava, 79.17 U ml^?1 (12 h) on potato and 55.37 U ml^?1 (in 6 h and max 77.75 U ml^?1 in 36 h) on cornstarch. Application of immobilization technique increased CGTase activity significantly. The immobilized cells produced CGTase with higher activity in a shorter fermentation time comparing to free cells.     

Does epigenetic polymorphism contribute to phenotypic variances in <Emphasis Type="Italic">Jatropha curcas</Emphasis> L.?

Background  

There is a growing interest in Jatropha curcas L. (jatropha) as a biodiesel feedstock plant. Variations in its morphology and seed productivity have been well documented. However, there is the lack of systematic comparative evaluation of distinct collections under same climate and agronomic practices. With the several reports on low genetic diversity in jatropha collections, there is uncertainty on genetic contribution to jatropha morphology.     

An environmental and economic analysis of the wood-pellet chain: two case studies in Southern Italy

Purpose

Wood pellet heating systems are considered as an essential component of European plans to reduce greenhouse gas (GHG) emissions. The goal of this analysis was to estimate and compare the environmental impacts and the costs of the production of packed wood pellets. Two pellet production systems, using roundwood logs (case 1) and mainly sawdust (case 2), have been analysed in 2015 in Basilicata region (Southern Italy).

Methods

A life cycle assessment (LCA) analysis was applied to calculate the environmental impact indicators of each system, whilst a life cycle cost (LCC) analysis was implemented to evaluate the pellets’ cost production. Hence, the functional unit chosen was 1 t of produced pellets. The system boundaries considered for the purpose of the current investigation were from the tree felling to the pellet packaging. In particular, the following activities were considered: motor-manual felling and delimbing with a chainsaw, timber yarding with a tractor along the forest track, loading and transportation of the logs to the collection point, transportation of timber to the factories for a distance of 35 km, pellet production and pellet packaging in low-density polyethylene bags with a total weight of 15 kg bag^?1.

Results and discussion

The production of 1 t of pellets emitted about 83 kg of CO₂eq in case 1 and 38 kg in case 2. In addition, 2.7 kg of SO₂eq and 0.005 kg of PO³ ₄-eq were produced in case 1 and 1.4 kg of SO₂eq and 0.002 kg of PO³ ₄-eq in case 2. Mineral extraction was equal to 0.9 MJ surplus energy in both cases. Case 1 led to higher environmental impacts (about 50% more), essentially for the operation of pelletisation, and in particular for the higher consumption of electricity that characterised it, whereas the production costs were 172 and 113 € t^?1 in case 1 and case 2, respectively. In both study cases, consumption costs (costs for raw material, electricity consumption, fuel usage) were the most important cost items.

Conclusions

Our studies highlight how, in both cases, the operations carried out in the forest produced the minor part of the environmental impact but, at the same time, were the most expensive operations. Further, our studies show how mixing lumbering by-products (sawdust) and forest management products (lumbers) can be an efficient solution to reduce both manufacturing costs and environmental impacts to produce wood pellets.

     

Attenuation of the vertical flux of copepod fecal pellets under Arctic sea ice: evidence for an active detrital food web in winter

A variable fraction of fecal pellets produced in the epipelagic layer is intercepted and retained before reaching the bottom. We assessed fecal pellet retention in the ice-covered Beaufort Sea in early February by comparing the shape and size-frequency distribution of pellets collected by a sediment trap moored at 210 m to that produced in vitro. Appendicularian ellipsoidal and copepod cylindrical pellets made up 75 and 24% of the flux (165 μg C m⁻² day⁻¹). In contrast, production (135 μg C m⁻² day⁻¹) was dominated by cylindrical pellets (93%). The vertical flux of cylindrical pellets at 210 m was attenuated by 70%. Pellets >120 μm in width, represented 42% of the production, but were not detected in the trap. Retention most likely resulted from coprorhexic feeding by copepods such as Metridia longa. Our observations suggest that the detritivore food web prevailing under the ice of the Arctic Ocean in winter is dominated by appendicularians feeding on pellets fragmented by copepods.     

Financial Analysis of the Cultivation of Short Rotation Woody Crops for Bioenergy in Belgium: Barriers and Opportunities

This paper analyses the financial performance of a poplar short rotation woody crop (SRWC) plantation in Belgium, from a farmer’s and an investor’s viewpoint, based on simulations from the newly developed model POPFINUA. The establishment, production and harvest costs were investigated to calculate the net present value (NPV) and the equivalent annual value (EAV) of the SRWC cultivation when the biomass chips were sold at a price of 40 € Mg^?1 with a moisture content (m.c.) of 50 %. The calculated NPVs were 229 and ?485 € ha^?1, and the EAVs equalled 16.3 and ?34.6 € ha^?1 year^?1 for the farmer’s and investor’s scenario, respectively. The break-even price at which the produced biomass could be sold at the farm gate excluding transport, handling, storage and profit margins of the involved companies was calculated using the levellised costs (LC) method and equalled 78.4 and 83.5 € oven-dried ton (odt)^?1 for the farmer’s and investor’s viewpoint, respectively. Three harvesting strategies, applied on a SRWC plantation of 18.1 ha in Flanders (Belgium), were studied and compared. It became clear that preference should be given to more economic, small-scale harvesters instead of large-scale self-propelled harvesters, given the relatively limited surface available for SRWCs in Belgium. Furthermore, the inclusion of transportation over a distance of 50 km by truck increased the LC by 15.1 € odt^?1. Moreover, subsidies such as establishment grants and/or yearly incentives proved indispensable to make this long-term investment profitable. This is particularly true for the scenario where an investor decides to cultivate SRWCs for energy purposes.