共查询到20条相似文献,搜索用时 31 毫秒
1.
Background
The repair of single base mutations in mammalian genes can be directed by single-stranded oligonucleotides in a process known as targeted gene repair. The mechanism of this reaction is currently being elucidated but likely involves a pairing step in which the oligonucleotide align in homologous register with its target sequence and a correction step in which the mutant base is replaced by endogenous repair pathways. This process is regulated by the activity of various factors and proteins that either elevate or depress the frequency at which gene repair takes place. 相似文献2.
Katrina L Tibballs Ole Herman Ambur Kristian Alfsnes H?vard Homberset Stephan A Frye Tonje Davidsen Tone T?njum 《BMC microbiology》2009,9(1):7
Background
Neisseria meningitidis, the causative agent of meningococcal disease, is exposed to high levels of reactive oxygen species inside its exclusive human host. The DNA glycosylase Fpg of the base excision repair pathway (BER) is a central player in the correction of oxidative DNA damage. This study aimed at characterizing the meningococcal Fpg and its role in DNA repair. 相似文献3.
Yunkui Zhu C Magnus Sköld Xiangde Liu Hangjun Wang Tadashi Kohyama Fu-Qiang Wen Ronald F Ertl Stephen I Rennard 《Respiratory research》2001,2(5):295-7
Background
Inflammatory cells are believed to play a prominent role during tissue repair and remodeling. Since repair processes develop and mature over extended time frames, the present study was designed to evaluate the effect of monocytes and fibroblasts in prolonged culture in three-dimensional collagen gels. 相似文献4.
Chih-Yen Wang Jen-Kun Chen Yi-Ting Wu May-Jywan Tsai Song-Kun Shyue Chung-Shi Yang Shun-Fen Tzeng 《Journal of biomedical science》2011,18(1):13
Background
Traumatic spinal cord injury (SCI) forms a disadvantageous microenvironment for tissue repair at the lesion site. To consider an appropriate time window for giving a promising therapeutic treatment for subacute and chronic SCI, global changes of proteins in the injured center at the longer survival time points after SCI remains to be elucidated. 相似文献5.
Background
Gene correction is an alternative approach to replacement gene therapy. By correcting mutations within the genome, some of the barriers to effective gene therapy are avoided. Homologous nucleic acid sequences can correct mutations by inducing recombination or mismatch repair. Recently, encouraging data have been presented using both short DNAfragments (SDFs) and RNA–DNA oligonucleotides (RDOs) in experimental strategies to realize clinical gene correction.Methods
The delivery of labelled SDFs and RDOs to a variety of cell lines was tested using both FACS analysis and confocal microscopy. A GFP‐based reporter system was constructed, containing a nonsense mutation, to allow quantitation of gene correction in living cells. This reporter was used to compare efficiencies of functional gene correction using SDFs and RDOs in arange of mammalian cell lines.Results
The delivery experiments highlight the inefficient delivery of SDFs and RDOs to the nucleus using polyethylenimine (PEI) transfection. This study compared the episomal correction efficiency of the reporter plasmid mediated by SDFs and RDOs within different cell types; low levels of functional correction were detected in cell culture.Conclusions
Whilst delivery of PEI‐complexed SDFs or RDOs to the cell is highly effective, nuclear entry appears to be a limiting factor. SDFs elicited episomal GFP correction across a range of cell lines, whereas RDOs only corrected the reporter in a cell line that overexpresses RAD51. Copyright © 2002 John Wiley & Sons, Ltd.6.
Mark D Robinson David P De Souza Woon Wai Keen Eleanor C Saunders Malcolm J McConville Terence P Speed Vladimir A Likić 《BMC bioinformatics》2007,8(1):419
Background
Gas chromatography-mass spectrometry (GC-MS) is a robust platform for the profiling of certain classes of small molecules in biological samples. When multiple samples are profiled, including replicates of the same sample and/or different sample states, one needs to account for retention time drifts between experiments. This can be achieved either by the alignment of chromatographic profiles prior to peak detection, or by matching signal peaks after they have been extracted from chromatogram data matrices. Automated retention time correction is particularly important in non-targeted profiling studies. 相似文献7.
Florian Böhrnsen Ulrich Lindner Markus Meier Abdelalim Gadallah Peter Schlenke Hendrik Lehnert Jürgen Rohwedel Jan Kramer 《BMC cell biology》2009,10(1):1-15
Background
Regenerative wound repair is a goal of modern medicine. This is important not only for the local repair but also for its beneficial effect to systemic physiological processes. When wounds become chronic, individuals are susceptible to generalized inflammatory cascades that can affect many organs and even lead to death. Skin is the most commonly injured tissue, and its proper repair is important for reestablishment of its barrier function. 相似文献8.
Kira S Makarova Nick V Grishin Svetlana A Shabalina Yuri I Wolf Eugene V Koonin 《Biology direct》2006,1(1):7-26
Background
All archaeal and many bacterial genomes contain Clustered Regularly Interspaced Short Palindrome Repeats (CRISPR) and variable arrays of the CRISPR-associated (cas) genes that have been previously implicated in a novel form of DNA repair on the basis of comparative analysis of their protein product sequences. However, the proximity of CRISPR and cas genes strongly suggests that they have related functions which is hard to reconcile with the repair hypothesis. 相似文献9.
Background
The development of software tools that analyze microarray data in the context of genetic knowledgebases is being pursued by multiple research groups using different methods. A common problem for many of these tools is how to correct for multiple statistical testing since simple corrections are overly conservative and more sophisticated corrections are currently impractical. A careful study of the nature of the distribution one would expect by chance, such as by a simulation study, may be able to guide the development of an appropriate correction that is not overly time consuming computationally. 相似文献10.
Background
The current investigation was undertaken to determine key steps differentiating G:T and G:A repair at the H-ras oncogenic hot spot within the nuclear environment because of the large difference in repair efficiency of these two mismatches. 相似文献11.
Kellie J Archer Catherine I Dumur G Scott Taylor Michael D Chaplin Anthony Guiseppi-Elie Geraldine Grant Andrea Ferreira-Gonzalez Carleton T Garrett 《BMC bioinformatics》2007,8(1):447
Background
Recent research examining cross-platform correlation of gene expression intensities has yielded mixed results. In this study, we demonstrate use of a correction factor for estimating cross-platform correlations. 相似文献12.
María Moreno-Villanueva Ragen Pfeiffer Thilo Sindlinger Alan Leake Marcus Müller Thomas BL Kirkwood Alexander Bürkle 《BMC biotechnology》2009,9(1):39-9
Background
Formation and repair of DNA single-strand breaks are important parameters in the assessment of DNA damage and repair occurring in live cells. The 'Fluorimetric Detection of Alkaline DNA Unwinding (FADU)' method [Birnboim HC, Jevcak JJ. Cancer Res (1981) 41:1889–1892] is a sensitive procedure to quantify DNA strand breaks, yet it is very tedious to perform. 相似文献13.
Wonmo Kang Tai-Il Kim Yerang Yun Hae-Won Kim Jun-Hyeog Jang 《Biotechnology letters》2011,33(1):199-204
Purpose of Work
We have developed a strategy of designing multi-functional extracellular matrix proteins for functionalizing bone tissue engineering scaffolds and other biomedical surfaces to achieve improvements in bone grafting, bone repair and bone regeneration. 相似文献14.
Background
Genome integrity is constantly challenged and requires the coordinated recruitment of multiple enzyme activities to ensure efficient repair of DNA lesions. We investigated the dynamics of XRCC1 and PCNA that act as molecular loading platforms and play a central role in this coordination. 相似文献15.
Carrie Fathke Lynne Wilson Kavita Shah Brian Kim Anne Hocking Randall Moon Frank Isik 《BMC cell biology》2006,7(1):4-9
Background
Cutaneous wound repair in adult mammals does not regenerate the original epithelial architecture and results in altered skin function. We propose that lack of regeneration may be due to the absence of appropriate molecular signals to promote regeneration. In this study, we investigated the regulation of Wnt signaling during cutaneous wound healing and the consequence of activating either the beta-catenin-dependent or beta-catenin-independent Wnt signaling on epidermal architecture during wound repair. 相似文献16.
Background
All animals possess some type of tissue repair mechanism. In some species, the capacity to repair tissues is limited to the healing of wounds. Other species, such as echinoderms, posses a striking repair capability that can include the replacement of entire organs. It has been reported that some mechanisms, namely extracellular matrix remodeling, appear to occur in most repair processes. However, it remains unclear to what extent the process of organ regeneration, particularly in animals where loss and regeneration of complex structures is a programmed natural event, is similar to wound healing. We have now used the sea cucumber Holothuria glaberrima to address this question. 相似文献17.
Background
Efficient and correct repair of DNA damage, especially DNA double-strand breaks, is critical for cellular survival. Defects in the DNA repair may lead to cell death or genomic instability and development of cancer. Non-homologous end-joining (NHEJ) is the major repair pathway for DNA double-strand breaks in mammalian cells. The ability of other repair pathways, such as homologous recombination, to compensate for loss of NHEJ and the ways in which contributions of different pathways are regulated are far from fully understood. 相似文献18.
Tristan Mary-Huard Julie Aubert Nadera Mansouri-Attia Olivier Sandra Jean-Jacques Daudin 《BMC bioinformatics》2008,9(1):98
Background
In individually dye-balanced microarray designs, each biological sample is hybridized on two different slides, once with Cy3 and once with Cy5. While this strategy ensures an automatic correction of the gene-specific labelling bias, it also induces dependencies between log-ratio measurements that must be taken into account in the statistical analysis. 相似文献19.
Ellen C Ordinario Munehisa Yabuki Priya Handa W Jason Cummings Nancy Maizels 《BMC molecular biology》2009,10(1):98-13
Background
Gene conversion depends upon the same factors that carry out more general process of homologous recombination, including homologous gene targeting and recombinational repair. Among these are the RAD51 paralogs, conserved factors related to the key recombination factor, RAD51. In chicken and other fowl, gene conversion (templated mutation) diversifies immunoglobulin variable region sequences. This allows gene conversion and recombinational repair to be studied using the chicken DT40 B cell line, which carries out constitutive gene conversion and provides a robust and physiological model for homology-directed repair in vertebrate cells. 相似文献20.
Laurent X Nouvel Tiago Dos Vultos Eric Kassa-Kelembho Jean Rauzier Brigitte Gicquel 《BMC microbiology》2007,7(1):39