共查询到20条相似文献,搜索用时 15 毫秒
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Rojas DA Moreira-Ramos S Zock-Emmenthal S Urbina F Contreras-Levicoy J Käufer NF Maldonado E 《The Journal of biological chemistry》2011,286(30):26480-26486
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In order to further characterize the previously observed disruptive effect of the RNA polymerase I promoter sequence (Pol
I) from Acanthamoeba castellanii on tandemly repeated 5S rDNA positioning sequences from sea urchin (Lytechinus variegatus), we compared the histone-binding ability of the isolated 199-bp Pol I promoter region to that of the 208-bp 5S rDNA and
that of nucleosome core particle sequences isolated from chicken erythocytes. We found the 5S rDNA positioning sequence to
be more efficient at forming nucleosomes than the RNA polymerase I promoter sequence. Nevertheless, examination of the free-DNA
half-depletion points during the titrations suggested that twice as much histone had bound to RNA polymerase I promoter sequence
as to the 5S nucleosome-positioning or core particle sequences. DNA bending analysis suggested two potential DNA bending loci
in the RNA polymerase I promoter, whereas only one such locus was predicted for the 5S positioning sequence. Such mixed bending
signals on the RNA polymerase I promoter could favor non-nucleosomal deposition of histones on these sequences. 相似文献
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The yeast transcription elongation factor Spt4/5 is a sequence‐specific RNA binding protein 下载免费PDF全文
Amanda J. Blythe Berra Yazar‐Klosinski Michael W. Webster Eefei Chen Marylène Vandevenne Katerina Bendak Joel P. Mackay Grant A. Hartzog Alice Vrielink 《Protein science : a publication of the Protein Society》2016,25(9):1710-1721
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