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1.
A novel bacterial strain, designated T-Y1 T, capable of degrading a variety of polysaccharides was isolated from seawater of an oyster farm in the South Sea, Korea. It was found to be aerobic, Gram-negative, non-flagellated, non-gliding and rod-shaped. Strain T-Y1 T grew optimally at 25 °C, at pH 7.0–7.5 and in the presence of 2 % (w/v) NaCl. A neighbour-joining phylogenetic tree based on 16S rRNA gene sequences showed that strain T-Y1 T belonged to the genus Winogradskyella. Strain T-Y1 T exhibited 16S rRNA gene sequence similarity values of 95.0–96.8 % to the type strains of recognized Winogradskyella species and less than 94.5 % to other validly named species. The chemotaxonomic data concurred with the phylogenetic inference. Strain T-Y1 T contained MK-6 as the predominant menaquinone and anteiso-C 15:0, iso-C 15:0, iso-C 15:1 G and iso-C 16:0 3-OH as the major fatty acids. The major polar lipids of strain T-Y1 T were phosphatidylethanolamine and two unidentified lipids. The DNA G+C content was 36.2 mol%. Differential phenotypic properties, together with its phylogenetic distinctiveness, enabled strain T-Y1 T to be differentiated from the recognized Winogradskyella species. On the basis of the data presented here, strain T-Y1 T is considered to represent a novel species of the genus Winogradskyella, for which the name Winogradskyella multivorans sp. nov. is proposed. The type strain is T-Y1 T (=KCTC 23891 T = CCUG 62216 T). 相似文献
2.
A novel aerobic mesophilic bacterial strain SYBC-H1 T capable of degrading chitin was isolated and classified in this study. The strain exhibited strong chitinolytic activity
and was a Gram-negative, curved, rod-shaped, and motile bacterium. Growth of this strain was observed between 10 and 41°C
and between pH 3.5 and 9.5. The DNA G + C content of strain SYBC-H1 T was 53.25 mol%. The cellular fatty acids (>5%) were 12:0 iso 3-OH (5.87%), 16:0 (28.16%), and 18:1ω7c (20.48%). Phylogenetic
analysis based on 16S rRNA gene sequence similarity revealed that strain SYBC-H1 T belonged to the family Neisseriaceae, and was distantly related (95.0% similarity) to the genus Chitiniphilus. Its phenotype was unique and genetic and phylogenetic analysis experiments suggested that strain SYBC-H1 T represented the type strain (CGMCC 3438 T, ATCC BAA-2140 T) of a novel genus, for which the name Chitinolyticbacter meiyuanensis SYBC-H1 T gen. nov., sp. nov. was proposed. The highest enzymatic activity of chitinase (9.6 U/ml) was obtained at 72 h in 250 ml shake
flasks. The 16S rRNA gene sequence of SYBC-H1 T has been deposited in GenBank under the accession number GQ981314. 相似文献
3.
A Gram-stain-negative, non-motile, rod-shaped bacterial strain, JW-64-1 T, capable of degrading methamidophos was isolated from a methamidophos-manufacturing factory in China, and was subjected to
a polyphasic taxonomic investigation. Strain JW-64-1 T produced circular, smooth, transparent, yellow-colored colonies (1.0–2.0 mm) on LB agar after 2 days incubation. It grew
optimally at 25–30°C and pH 7.0 without the presence of NaCl. The G+C content of the total DNA was 63.6 mol%. A phylogenetic
analysis based on 16S rRNA gene sequences showed that strain JW-64-1 T fell within the cluster comprising Luteibacter species. The 16S rRNA gene sequence of strain JW-64-1 T was most closely related to Luteibacter rhizovicinus DSM 16549 T (98.6%), followed by Luteibacter
yeojuensis DSM 17673 T (98.4%) and L. anthropi CCUG 25036 T (98.2%). The major cellular fatty acids of strain JW-64-1 T were iso-C 15:0 (24.1%), iso-C 17:0 (20.2%) and summed feature 9 comprising iso-C 17:1 ω9c and/or C 16:0 10-methyl (20.3%). The major isoprenoid quinine was Q-8 (98%), and the major polar lipids were diphosphatidylglycerol, phosphatidylglycerol,
phosphoaminolipid, aminolipids-1, aminolipids-2, and phospholipids. The values for DNA–DNA relatedness between strain JW-64-1 T and the closest phylogenetic relatives of L. rhizovicinus and Luteibacter
yeojuensis were 34.8 ± 2.6 and 25.6 ± 3.1%, respectively. On the basis of the phenotypic, chemotaxonomic, DNA–DNA relatedness and phylogenetic
analysis based on the 16S rRNA gene sequences, strain JW-64-1 T represents a novel species of the genus Luteibacter, for which the name Luteibacter jiangsuensis sp. nov. is proposed. The type strain is JW-64-1 T (=CGMCC 1.10133 T = DSM 22396 T). 相似文献
4.
A Gram-positive, non-pigmented, rod-shaped, diazotrophic bacterial strain, designated SC-N012 T, was isolated from rhizosphere soil of sugarcane and was subjected to a polyphasic taxonomic study. The strain exhibited
phenotypic properties that included chemotaxonomic characteristics consistent with its classification in the genus Bacillus. Sequence analysis of the 16S rRNA gene of SC-N012 T revealed the closest match (98.9% pair wise similarity) with Bacillus clausii DSM 8716 T. However, DNA–DNA hybridization experiments indicated low levels of genomic relatedness (32%) with this strain. The major
components of the fatty acid profile are iso-C 15:0, anteiso-C 15:0, iso-C 17:0 and anteiso-C 17:0. The diagnostic cell-wall diamino acid was meso-diaminopimelic acid. The G+C content of the genomic DNA is 43.0 mol%. The lipids present in strain SC-N012 T are diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol and two unknown phospholipids. Their predominant
respiratory quinone was MK-7. Studies of DNA-DNA relatedness, morphological, physiological and chemotaxonomic analyses and
phylogenetic data based on 16S rRNA gene sequencing allowed strain SC-N012 T to be described as members of novel species of the genus Bacillus, for which the name Bacillus rhizosphaerae sp. nov. is proposed. The type strain is SC-N012 T (=DSM 21911 T = NCCB 100267 T). 相似文献
5.
Two bacterial strains, BT325T and BT690, were isolated from soil samples collected in Korea. Both strains were Gram stain-negative, short rod-shaped, and formed light-pink colored colonies. The 16S rRNA sequence similarity of strains BT325T and BT690 shared a sequence similarity of 99.7%. Both strains shared the highest 16S rRNA gene similarity of 98.6% with Microvirga arabica SV2184PT, followed by Microvirga ossetica V5/3 M T (98.5% and 98.2%, respectively), Microvirga soli R491T (98.3% and 98.2%, respectively), Microvirga aerilata (98.2% and 98.08%, respectively), Microvirga makkahensis (98.08% and 97.8%, respectively). Phylogenetic analyses based on 16S rRNA gene sequences revealed that strain BT325T and BT690 were positioned in a distinct lineage within the family Methylobacteriaceae (order Rhizobiales, class Alphaproteobacteria). The genome size of strain BT325T was 5,200,315 bp and the genomic DNA G?+?C content was 64.3 mol%. The sole respiratory quinone of strain BT325T was Q-10 and the predominant cellular fatty acids were summed feature 3 (C16:1 ω7c/C16:1 ω6c) and summed feature 8 (C18:1 ω7c/C18:1 ω6c). The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, and phosphatidylcholine. Polyphasic taxonomic analysis of biochemical, chemotaxonomic, and phylogenetic analyses suggested that strains BT325T represents a novel bacterial species within the genus Microvirga, for which the name Microvirga splendida is proposed. The type strain of Microvirga splendida is BT325T (=?KCTC 72406 T?=?NBRC 114847 T). 相似文献
6.
A gram-negative, non-flagellated and ovoid- to rod-shaped bacterial strain, designated GSW-M15 T, was isolated from seawater on the southern coast of South Korea. Strain GSW-M15 T grew optimally at 30 °C, at pH 7.0–7.5 and in the presence of 2 % (w/v) NaCl. The phylogenetic trees based on 16S rRNA gene sequences revealed that strain GSW-M15 T belonged to the genus Roseovarius. Strain GSW-M15 T exhibited highest 16S rRNA gene sequence similarity values (98.3 and 97.5 %) to Roseovarius halotolerans HJ50 T and Roseovarius pacificus 81-2 T and 92.8-96.2 % sequence similarity values to the type strains of the other Roseovarius species. Strain GSW-M15 T contained Q-10 as the predominant ubiquinone and C 18:1 ω7 c and 11-methyl-C 18:1 ω7 c as the major fatty acids. The major polar lipids detected in strain GSW-M15 T were phosphatidylcholine, phosphatidylglycerol, phosphatidylethanolamine, one unidentified aminolipid and two unidentified lipids. The DNA G+C content of strain GSW-M15 T was 62.9 mol% and its mean DNA–DNA relatedness values with R. halotolerans KCTC 22224 T and R. pacificus LMG 24575 T were 33 and 18 %, respectively. Differential phenotypic properties of strain GSW-M15 T, together with the phylogenetic and genetic distinctiveness, demonstrated that this strain is distinguishable from other Roseovarius species. On the basis of the data presented here, strain GSW-M15 T (=KCTC 23897 T = CCUG 62218 T) represents a novel species of the genus Roseovarius, for which the name Roseovarius litoreus sp. nov. is proposed. 相似文献
7.
A Gram-staining positive, endospore-forming, motile and rod-shaped bacterial strain, BR-29 T, was isolated from soil from west coast of the Korean peninsula, and its taxonomic position was investigated by a polyphasic
study. Strain BR-29 T grew optimally at around pH 7.5, at 30°C and in the presence of 0.5% (w/v) NaCl. Phylogenetic analyses based on 16S rRNA
gene sequences showed that strain BR-29 T fell into a clade comprising the type strains of Cohnella species, with which it exhibited 16S rRNA gene sequence similarity values of 92.8–96.4%. Strain BR-29 T contained a cell wall peptidoglycan based on meso-diaminopimelic acid and MK-7 as the predominant menaquinone. The major fatty acids were anteiso-C 15:0, C 16:0 and iso-C 16:0. The major polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, lysylphosphatidylglycerol and two unidentified
phospholipids; a minor amount of phosphatidylglycerol was present. The DNA G+C content was 54.9 mol%. Strain BR-29 T could be differentiated from phylogenetically related Cohnella species by differences in phenotypic characteristics. On the basis of phenotypic, chemotaxonomic and phylogenetic data, strain
BR-29 T represents a novel species of the genus Cohnella, for which the name Cohnella
boryungensis sp. nov., is proposed. The type strain is BR-29 T (= KCTC 13735 T = CCUG 59598 T). 相似文献
8.
Strain Gsoil 348 T was isolated from a ginseng field soil sample by selecting micro-colonies from one-fifth strength modified R2A agar medium after a long incubation period. 16S rRNA gene sequence analysis indicated that the strain is related to members of the phylum Armatimonadetes (formerly called candidate phylum OP10). Strain Gsoil 348 T is mesophilic, strictly aerobic, non-motile and rod-shaped. It only grows in low nutrient media. The major respiratory quinones are menaquinones MK-11 and MK-10, and the main fatty acids are iso-C 15:0, iso-C 17:0, C 16:0 and C 16:1 ω11 c. The G+C content is 61.4 mol%. The 16S rRNA gene sequences in public databases belonging to the phylum Armatimonadetes were clustered here into 6 groups. Five of these groups constituted a coherent cluster distinct from the sequences of other phyla in phylogenetic trees that were constructed using multiple-outgroup sequences from 49 different phyla. On the basis of polyphasic taxonomic analyses, it is proposed that strain Gsoil 348 T (= KACC 14959 T = JCM 17079 T) should be placed in Fimbriimonas ginsengisoli gen. nov., sp. nov., as the cultured representative of the Fimbriimonadia class. nov., corresponding with Group 4 of the phylum Armatimonadetes. 相似文献
9.
A novel aerobe thermophilic endospore-forming bacterium designated strain AF/04 T was isolated from thermal mud located in Euganean hot springs, Abano Terme, Padova, Italy. Strain AF/04 T was Gram-positive, motile, rod-shaped, occurring in pairs, or filamentous. The isolate grew between 55 and 67°C (optimum
65°C) and at pH 6.0–7.5 (optimum pH 7.2). The strain was aerobic and grew on maltose, trehalose, and sodium acetate as sole
carbon sources. The G + C content of DNA was 53.5 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that
strain AF/04 T falls within the genus Anoxybacillus. Levels of 16S rRNA gene sequence similarity between strain AF/04 T and the type strains of recogni zed Anoxybacillus species ranged from 95 to 99%. Chemotaxonomic data (major isoprenoid quinone–menaquinone-7; major fatty acid iso-C15:0 and anteiso-C17:0) supported the affiliation of strain AF/04 T to the genus Anoxybacillus. Based on phenotypic and chemotaxonomic characteristics, 16S rRNA gene sequence analysis and DNA–DNA hybridi zation data, it was proposed that strain AF/04 T (=DSM 17141 T = ATCC BAA 1156 T) should be placed in the genus Anoxybacillus as the type strain of a novel species, Anoxybacillus thermarum sp. nov. 相似文献
10.
A Gram-staining-negative, oxidase-positive, catalase-positive, non-motile, non-spore-forming, and rod-shaped bacterium, designated
BJQ-6 T, was isolated from activated sludge of a waste-water treatment plant in Jiangsu Province, China. Phylogenetic analysis based
on 16S rRNA gene sequences showed that strain BJQ-6 T belonged to the genus Rhodanobacter, and shared 98.7% similarity with Rhodanobacter lindaniclasticus LMG 18385 T and <97% similarities with other Rhodanobacter species. The major fatty acids were iso-C 15:0 (17.6%), iso-C 16:0 (19.3%), and Summed feature 9 (isoC 17:1
ω9c and/or C 16:0 10-methyl) (25.8%). The DNA G+C content of strain BJQ-6 T was 64.8 mol%. Based on the phenotypic and phylogenetic considerations, strain BJQ-6 T represents a novel species of the genus Rhodanobacter, for which the name Rhodanobacter xiangquanii sp. nov. is proposed. The type strain is BJQ-6 T (=CCTCC AB 2010106 T =KCTC 23100 T). 相似文献
11.
An aerobic, Gram-stain-negative, non-motile, non-spore-forming, rod-shaped, and light pink-colored bacterial strain, designated TS19T, was isolated from a sand sample obtained from a coastal sand dune after exposure to 3 kGy of gamma radiation. Phylogenetic analysis based on the 16S rRNA gene sequences revealed that the isolate was a member of the genus Hymenobacter and was most closely related to H. wooponensis WM78T (98.3% similarity). Strain TS19T and H. wooponensis showed resistance to gamma radiation with D10 values (i.e., the dose required to reduce the bacterial population by tenfold) of 7.3 kGy and 3.5 kGy, respectively. The genome of strain TS19T consists of one contig with 4,879,662 bp and has a G?+?C content of 56.2%. The genome contains 3,955 protein coding sequences, 44 tRNAs, and 12 rRNAs. The predominant fatty acids of strain TS19T were iso-C15:0, summed feature 4 (iso-C17:1 I and/or anteiso-C17:1 B), summed feature 3 (C16:1 ω6c and/or C16:1 ω7c), and C16:1 ω5c. The major polar lipids were phosphatidylethanolamine, and one unidentified aminophospholipid. The main respiratory quinone was menaquinone-7. Based on the phylogenetic, physiological, and chemotaxonomic characteristics, strain TS19T represents a novel species, for which the name Hymenobacter taeanensis sp. nov. is proposed. The type strain is TS19T (=?KCTC 72897T?=?JCM 34023T). 相似文献
12.
Strain CBA3638T was isolated from the Geum River sediment, Republic of Korea. The cells of strain CBA3638T were Gram-stain-positive, strictly anaerobic, rod-shaped, and 0.5–1.0 μm wide, and 4.0–4.5 μm long. Optimal growth occurred at 37 °C, pH 7.0, and 1.0% (w/v) NaCl. Based on the 16S rRNA gene sequence, the phylogenetic analysis showed that strain CBA3638T belongs to the genus Anaerocolumna in the family Lachnospiraceae, and is most closely related to Anaerocolumna cellulosilytica (94.6–95.0%). The DDH value with A. cellulosilytica SN021T showed 15.0% relatedness. The genome of strain CBA3638T consisted of one circular chromosome that is 5,500,435 bp long with a 36.7 mol% G?+?C content. The genome contained seven 16S-5S-23S rRNA operons and one antibiotic resistance-related transporter gene (mefA). Quinones were not detected. The predominant cellular fatty acids were C16:0 and C14:0 and the polar lipids were diphosphatidylglycerol, phosphatidylcholine, and uncharacterised polar lipids. Based on the polyphasic taxonomic analysis, we propose strain CBA3638T as a novel species in the genus Anaerocolumna, with the name Anaerocolumna sedimenticola sp. nov. The type strain is CBA3638T (=?KACC 21652T?=?DSM 110663T). 相似文献
13.
A Gram-negative, motile and rod-shaped bacterial strain, designated S7-3 T, was isolated from a tidal flat sediment at Saemankum on the western coast of Korea. Phylogenetic analyses based on 16S rRNA gene and gyrB sequences showed that strain S7-3 T belonged to the genus Shewanella, clustering with Shewanella decolorationis S12 T. Strain S7-3 T exhibited 98.8 % 16S rRNA gene sequence similarity and 96.8 % gyrB sequence similarity to S. decolorationis S12 T, respectively. The 16S rRNA gene sequence similarity values between strain S7-3 T and other members of the genus Shewanella were in the range of 93.0–98.0 %. Strain S7-3 T contained simultaneously both menaquinones (MK) and ubiquinones (Q); the predominant menaquinone was MK-7 and the predominant ubiquinones were Q-7 and Q-8. The fatty acid profiles of strain S7-3 T and S. decolorationis JCM 21555 T were similar; major components were C 17:1 ω8 c, iso-C 15:0 and iso-C 15:0 2-OH and/or C 16:1 ω7 c. The DNA G+C content of strain S7-3 T was 51.8 mol% and its mean DNA–DNA relatedness value with S. decolorationis JCM 21555 T was 43 %. Differential phenotypic properties of strain S7-3 T, together with the phylogenetic and genetic distinctiveness, revealed that this strain is distinguishable from recognized Shewanella species. On the basis of the data presented, strain S7-3 T is considered to represent a novel Shewanella species, for which the name Shewanella seohaensis sp. nov. is proposed. The type strain is S7-3 T (=KCTC 23556 T = CCUG 60900 T). 相似文献
14.
A novel Gram-positive, aerobic, rod-shaped and mycelia-producing bacterial strain, designated KLBMP 1050 T, was isolated from the stem of the oil-seed plant Jatropha curcas L. collected from Sichuan Province, south-west China. Phylogenetic analysis based on the 16S rRNA gene sequence revealed
that the isolate KLBMP 1050 T belonged to the genus Nocardioides, with the highest sequence similarity to Nocardioides albus KCTC 9186 T (99.38 %) and Nocardioides luteus KCTC 9575 T (99.03 %). However, the DNA–DNA relatedness of isolate KLBMP 1050 T to these two type strains were 37.5 ± 3.5 and 33 ± 2.3 %, respectively. Strain KLBMP 1050 T grew at the pH range 6–11, temperature range 10–32 °C and with 0–12 % NaCl. The physiological properties of strain KLBMP
1050 T differ from those of N. albus KCTC 9186 T and N. luteus KCTC 9575 T. The cell-wall peptidoglycan contained ll-diaminopimelic acid and MK-8(H 4) was the major respiratory quinone. The predominant cellular fatty acid of strain KLBMP 1050 T was iso-C 16:0 (23.3 %). The total DNA G+C content was 70.1 mol%. On the basis of the phenotypic, chemotaxonomic and phylogenetic data,
strain KLBMP 1050 T represents a novel species of the genus Nocardioides, for which the name Nocardioides panzhihuaensis sp. nov. is proposed. The type strain is KLBMP 1050 T (= KCTC 19888 T = NBRC 108680 T). 相似文献
15.
A bacterial strain designated antisso-27 T, previously isolated from saltpan in Taiwan while screening for bacteria for algicidal activity, was characterized using
the polyphasic taxonomic approach. Strain antisso-27 T was Gram-negative, aerobic, brownish yellow colored, rod-shaped, non-flagellated and non-gliding. Phylogenetic analyses based
on 16S rRNA gene sequences showed that strain antisso-27 T belonged to the genus Aquimarina within the family Flavobacteriaceae with relatively low sequence similarities of 94.0–96.6% to other valid Aquimarina spp. It contained iso-C 17:0 3-OH, iso-C 15:0, iso-C 16:0, iso-C 15:1 and iso-C 15:0 3-OH as the main fatty acids and contained a menaquinone with six isoprene units (MK-6) as the major isoprenoid quinone.
Major polar lipids were phosphatidylethanolamine, diphosphatidylglycerol, an uncharacterized aminolipid and five uncharacterized
phospholipids. Strain antisso-27 T employed direct mode of algicidal lysis to Chlorella vulgaris strain 211-31; nevertheless, it released an algicidal substance against M. aeruginosa strain MTY01. This is the first study that the Aquimarina species possesses both direct and indirect algicidal activities. On the basis of the phylogenetic and phenotypic data, strain
antisso-27 T should be classified as representing a novel species, for which the name A. salinaria sp. nov. is proposed. The type strain is A. salinaria antisso-27 T (= BCRC 80080 T = LMG 25375 T). 相似文献
16.
A novel Gram-negative and rod-shaped bacterium, designated N8 T, was isolated from tidal flat sediment. Phylogenetic analysis based on 16S rRNA gene sequences showed that N8 T strain is associated with the family Phyllobacteriaceae: two uncultured clones (98.4 and 99.8% 16S rRNA gene sequence similarity) and the genus Mesorhizobium (≤97.0%). The novel strain formed a separate clade with uncultured clones in the phylogenetic tree based on 16S rRNA gene
sequences. Cellular fatty acid profiles predominately comprised C 18:1
ω7 c and C 19:0 cyclo ω8 c. The major isoprenoid quinone is ubiquinone-10 and genomic DNA G+C content is 53.4 mol%. The polyphasic taxonomic study indicates
that the novel strain N8 T represents a novel species of the new genus in the family Phyllobacteriaceae, named Aliihoeflea aestuarii. The type strain is N8 T (= KCTC 22052 T= JCM 15118 T= DSM 19536 T). 相似文献
17.
A Gram-negative, facultatively anaerobic, motile by means of single polar flagellum, rod-shaped marine bacterium, designated
strain E414, was isolated from sea water collected from a farming pond rearing marine shrimp Litopenaeus vannamei in Zhanjiang, Guangdong province, PRC. The strain was able to grow in the presence of 0.5–6% (w/v) NaCl (optimally in 3–6%
(w/v) NaCl), between pH 6 and 9 (optimally at pH 7–8), between 15 and 37°C (optimally at 25–30°C). Phylogenetic analysis based
on 16S rRNA gene sequences locate strain E414 in the vicinity of the coralliilyticus clade within the genus Vibrio. DNA–DNA relatedness data and multigene phylogenetic analysis based on the concatenated sequences of four genes (16S rRNA,
rpoA, recA and pyrH) clearly differentiated strain E414 from its closest phylogenetic neighbours. Analysis of phenotypic features, including
enzyme activities and utilization and fermentation of various carbon sources, further revealed discrimination between strain
E414 and phylogenetically related Vibrio species. The major fatty acid components are C 16:1ω6c and/or C 16:1ω7c (27.4%), C 18:1ω7c and/or C 18:1ω6c (19.3%) and C 16:0 (18.2%). The DNA G+C content of strain E414 was 38.7 mol%. Based on phenotypic, chemotaxonomic, phylogenetic and DNA–DNA
relatedness values, it can be concluded that E414 should be placed in the genus Vibrio as representing a novel species, for which the name Vibrio zhanjiangensis sp. nov. is proposed, with the type strain E414 (=CCTCC AB 2011110 T = NBRC 108723 T = DSM 24901). 相似文献
18.
A Gram-stain-positive, orange-pigmented, rod-shaped and flagellated bacterial strain T12T was isolated from wetland soil in Kunyu Mountain Wetland in Yantai, China. The strain was able to grow at 15–40 °C (optimum 37 °C), at 0.0–9.0% NaCl (optimum 2%, w/v) and at pH 5.5–9.0 (optimum 8.5). A phylogenetic analysis based on the 16S rRNA gene sequence indicated that strain T12T is a member of the family Planococcaceae, sharing 97.6% and 97.1% sequence similarity with the type strains of Jeotgalibacillus salarius and Jeotgalibacillus marinus, respectively. Genome-based analyses revealed a genome size of 3,506,682 bp and a DNA G?+?C content of 43.7%. Besides, the genome sequence led to 55.0–74.6% average amino acid identity values and 67.8–74.7% average nucleotide identity values between strain T12T and the current closest relatives. Digital DNA-DNA hybridization of strain T12T with the type strains of Jeotgalibacillus proteolyticus and J. marinus demonstrated 19.0% and 20.3% relatedness, respectively. The chemotaxonomic analysis showed that the sole quinone was MK-7. The predominant cellular fatty acids were iso-C15:0, anteiso-C15:0, C16:1ω7c alcohol and iso-C14:0. The polar lipids consisted of an unidentified aminolipid, phosphatidylglycerol, diphosphatidylglycerol and two unidentified phospholipids. Based on the polyphasic characterization, strain T12T is considered to represent a novel species, for which the name Jeotgalibacillus aurantiacus sp. nov. is proposed. The type strain is T12T (=?KCTC 43296 T?=?MCCC 1K07171T). 相似文献
19.
A novel bacterium, designated Z-25 T, was isolated from a rice paddy rhizosphere soil sample from Wuchang County, China. The Z-25 T strain is gram-negative, rod-shaped, non-spore-forming, aerobic, motile by unipolar flagella and straw white in color. A phylogenetic analysis based on the 16S rRNA gene sequence revealed that strain Z-25 belongs to the genus Shinella, and the closest members are Shinella zoogloeoides ATCC 19623 T with 98.58% similarity, S. kummerowiae CCBAU 25,048 T (98.03%) and S. granuli Ch06 T (97.37%). The average nucleotide identity and in silico DNA-DNA hybridization values between strain Z-25 T and the closest members were less than 85.29% and 28.70%, respectively. The predominant fatty acids were the sums of features comprising C18:1 ω7c and/or C18:1 ω6c (34.62%), C18:1 ω7c -11-methyl (20.48%), and C19:0 cyclo ω8c (18.19%). The only respiratory quinone was ubiquinone-10, and the major polar lipids were diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. Additionally, a genome analysis showed that Z-25 T presented potential functional genes related to the degradation of zearalenone (ZEN). An HPLC analysis indicated that Z-25 T could remove 74.13% of 10 mg/L ZEN after 144 h at 30 °C. Therefore, based on phenotypic, chemotaxonomic, phylogenetic and genotypic analyses, strain Z-25 T represents a novel species in the genus Shinella, for which the name Shinella oryzae sp. nov. is proposed. The type strain is Z-25 T (=?GDMCC 1.2424 T?=?KCTC 82660 T). 相似文献
20.
A novel cold-resistant bacterium, designated YIM 016 T, was isolated from a peat bog sample collected from Mohe County, Heilongjiang Province, Northern China and its taxonomic position was investigated using a polyphasic approach. The strain was Gram-positive, aerobic, endospore-forming, motile and rod-shaped. Phylogenetic analyses based on the 16S rRNA gene sequence clearly revealed that strain YIM 016 T is a member of the genus Paenibacillus. The strain is closely related to Paenibacillus alginolyticus DSM 5050 T, Paenibacillus chondroitinus DSM 5051 T and Paenibacillus pocheonensis Gsoil 1138 T with similarities of 99.0 %, 97.0 % and 96.3 %, respectively. Meanwhile, the low DNA–DNA relatedness levels between strain YIM 016 T and its closely related phylogenetic neighbours demonstrated that this isolate represents a new genomic species in the genus Paenibacillus. Phenotypic and chemotaxonomic tests showed that growth of strain YIM 016 T occurred at 4–37 °C, pH 6.0–8.0 and with a NaCl tolerance up to 0.5 % (w/v). The peptidoglycan contained meso-diaminopimelic acid, alanine and glutamic acid. The whole-cell hydrolysates mainly contained glucose, galactose and ribose. The predominant menaquinone was MK-7 and the major fatty acids were anteiso-C 15:0 and iso-C 16:0. The DNA G+C content of strain YIM 016 T was 51.7 mol %. On the basis of phylogenetic, phenotypic and chemotaxonomic characteristics, strain YIM 016 T could be clearly distinguished from other species of the genus Paenibacillus. It is therefore concluded that strain YIM 016 T represents a novel species in the genus Paenibacillus, for which the name Paenibacillus frigoriresistens sp. nov. is proposed. The type strain is YIM 016 T (= CCTCC AB 2011150 T = JCM 18141 T). 相似文献
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