共查询到20条相似文献,搜索用时 15 毫秒
1.
R. Heller J. Schondelmaier G. Steinrücken C. Jung 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1996,92(8):991-997
Sugar beet (Beta vulgaris L.) is highly susceptible to the beet cyst nematode (Heterodera schachtii Schm.). Three resistance genes originating from the wild beets B. procumbens (Hs1
pro-1) and B. webbiana (Hs1
web-1, Hs2
web-7) have been transferred to sugar beet via species hybridization. We describe the genetic localization of the nematode resistance genes in four different sugar beet lines using segregating F2 populations and RFLP markers from our current sugar beet linkage map. The mapping studies yielded a surprising result. Although the four parental lines carrying the wild beet translocations were not related to each other, the four genes mapped to the same locus in sugar beet independent of the original translocation event. Close linkage (0–4.6 cM) was found with marker loci at one end of linkage group IV. In two populations, RFLP loci showed segregation distortion due to gametic selection. For the first time, the non-randomness of the translocation process promoting gene transfer from the wild beet to the sugar beet is demonstrated. The data suggest that the resistance genes were incorporated into the sugar beet chromosomes by non-allelic homologous recombination. The finding that the different resistance genes are allelic will have major implications on future attempts to breed sugar beet combining the different resistance genes. 相似文献
2.
M. Kleine H. Voss D. Cai C. Jung 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1998,97(5-6):896-904
Thirty sugar beet (Beta vulgaris) lines conferring complete resistance to the beet cyst nematode (BCN, Heterodera schachtii) originating from interspecific crosses with wild beets of the section Procumbentes (B. procumbens, B. webbiana and B. patellaris) were investigated by morphology and wild beet-specific molecular markers. The beet lines carrying chromosome mutations consisted
of monosomic additions (2n=18+1), fragment additions (2n=18+fragment) and translocations (2n=18) from the wild beets. Genome-specific
single-copy, satellite and repetitive probes were applied to study the origin, chromosomal assignment and presence of nematode
resistance genes. Within the wild beet species at least three different resistance genes located on different chromosomes
were distinguished: Hs1 on the homoelogous chromosomes I of each species, Hs2 on the homoelogous chromosomes VII of B. procumbens and B. webbiana and Hs3 on chromosome VIII of B. webbiana. A clear distinction between the three chromosomes was possible by morphological and molecular means. The translocation lines
were separated into two different groups: one containing the resistance gene Hs1 from chromosome I and the other carrying a different nematode resistance gene. The molecular data combined with sequence
analyses of Hs1 of the three wild beet species revealed a clear distinction between B. procumbens and B. webbiana. The evolutionary and taxonomical relationship of these species supporting the idea of three different species originating
from a common ancestor is discussed.
Received: 6 April 1998 / Accepted: 22 April 1998 相似文献
3.
Taguchi K Ogata N Kubo T Kawasaki S Mikami T 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2009,118(2):227-234
Aphanomyces root rot, caused by Aphanomyces cochlioides Drechs., is one of the most serious diseases of sugar beet (Beta vulgaris L.). Identification and characterization of resistance genes is a major task in sugar beet breeding. To ensure the effectiveness
of marker-assisted screening for Aphanomyces root rot resistance, genetic analysis of mature plants’ phenotypic and molecular
markers’ segregation was carried out. At a highly infested field site, some 187 F2 and 66 F3 individuals, derived from a cross between lines ‘NK-310mm-O’ (highly resistant) and ‘NK-184mm-O’ (susceptible), were tested,
over two seasons, for their level of resistance to Aphanomyces root rot. This resistance was classified into six categories
according to the extent and intensity of whole plant symptoms. Simultaneously, two selected RAPD and 159 ‘NK-310mm-O’-coupled
AFLP were used in the construction of a linkage map of 695.7 cM. Each of nine resultant linkage groups was successfully anchored
to one of nine sugar beet chromosomes by incorporating 16 STS markers. Combining data for phenotype and molecular marker segregation,
a single QTL was identified on chromosome III. This QTL explained 20% of the variance in F2 population (in the year 2002) and 65% in F3 lines (2003), indicating that this QTL plays a major role in the Aphanomyces root rot resistance. This is the first report
of the genetic mapping of resistance to Aphanomyces-caused diseases in sugar beet. 相似文献
4.
R. Delourme N. Foisset R. Horvais P. Barret G. Champagne W. Y. Cheung B. S. Landry M. Renard 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1998,97(1-2):129-134
Bulked segregant analysis and comparative mapping were applied to identify molecular markers linked to the Rfo restorer gene used for the Ogu-INRA cytoplasmic male-sterility system in rapeseed. These markers were then used to localise the radish introgression on
the B. napus genetic map constructed from the cross ‘Darmor.bzh’ x ’Yudal’. The introgression mapped on the DY15 linkage group. From the comparison of this latter group to the linkage group
constructed on a F2 progeny segregating for the radish introgression, it was concluded that the introgression had occurred through homoeologous
recombination, that it was not distal and that it had replaced a B. napus region of around 50 cM. A QTL involved in aliphatic seed glucosinolate content was located on the DY15 linkage group at a
position corresponding to one end of the introgression. The DNA markers identified in this study are being used in map-based
cloning of the Rfo gene and in marker-assisted selection.
Received: 3 December 1997 / Accepted: 17 December 1997 相似文献
5.
Mapping QTLs for submergence tolerance in rice by AFLP analysis and selective genotyping 总被引:11,自引:0,他引:11
S. Nandi P. K. Subudhi D. Senadhira N. L. Manigbas S. Sen-Mandi N. Huang 《Molecular & general genetics : MGG》1997,255(1):1-8
By combining the amplified fragment length polymorphism (AFLP) technique with selective genotyping, we constructed a linkage
map for rice and assigned each linkage group to a corresponding chromosome. The AFLP map, consisting of 202 AFLP markers,
was generated from 74 recombinant inbred lines (RIL) which were selected from both extremes of the population (250 lines)
with respect to the response to complete submergence. Map length was 1756 cM, with an average interval size of 8.5 cM. To
assign linkage groups to chromosomes, we used 50 previously mapped AFLP markers as anchor markers distributed over the 12
chromosomes. Other AFLP markers were then assigned to specific chromosomes based on their linkage to anchor markers. This
AFLP map is equivalent to the RFLP/AFLP map constructed previously as the anchors were in the same order in both maps. Furthermore,
tests with two restriction fragment length polymorphism (RFLP) markers and two sequence-tagged site (STS) markers showed that
they mapped in the expected positions. Using this AFLP map, a major gene for submergence tolerance was localized on chromosome
9. Quantitative trait loci (QTL) associated with submergence tolerance were detected on chromosomes 6, 7, 11, and 12. We conclude
that the combination of AFLP mapping and selective genotyping provides a much faster and easier approach to QTL identification
than the use of RFLP markers.
Received: 20 December 1996 / Accepted: 21 January 1997 相似文献
6.
Sishen Li Jizeng Jia Xianyun Wei Xiaocun Zhang Linzhi Li Haimei Chen Yuding Fan Haiyan Sun Xinhua Zhao Tiandong Lei Yunfong Xu Fangshan Jiang Honggang Wang Lihui Li 《Molecular breeding : new strategies in plant improvement》2007,20(2):167-178
A new genetic linkage map was constructed based on recombinant inbred lines (RILs) derived from the cross between the Chinese
winter wheat (Triticum aestivum L.) varieties, Chuang 35050 and Shannong 483 (ChSh). The map included 381 loci on all the wheat chromosomes, which were composed
of 167 SSR, 94 EST-SSR, 76 ISSR, 26 SRAP, 15 TRAP, and 3 Glu loci. This map covered 3636.7 cM with 1327.7 cM (36.5%), 1485.5 cM (40.9%), and 823.5 cM (22.6%) for A, B, and D genome,
respectively, and contained 13 linkage gaps. Using the RILs and the map, we detected 46 putative QTLs on 12 chromosomes for
grain yield (GY) per m2, thousand-kernel weight (TKW), spike number (SN) per m2, kernel number per spike (KNS), sterile spikelet number per spike (SSS), fertile spikelet number per spike (FSS), and total
spikelet number per spike (TSS) in four environments. Each QTL explained 4.42–70.25% phenotypic variation. Four QTL cluster
regions were detected on chromosomes 1D, 2A, 6B, and 7D. The most important QTL cluster was located on chromosome 7D near
the markers of Xwmc31, Xgdm67, and Xgwm428, in which 8 QTLs for TKW, SN, SSS and FSS were observed with very high contributions (27.53–67.63%). 相似文献
7.
Liu Fenglou Gupta Sanjiv Zhang Xiao-Qi Jones Michael Loughman Robert Lance Reg Li Chengdao 《Molecular breeding : new strategies in plant improvement》2011,28(4):657-666
Adult plant resistance (APR) is considered potentially more durable for controlling barley leaf rust than seedling Rph (Resistance to Puccinia hordei) genes. A major gene for adult plant resistance to barley leaf rust has been mapped to the telomere region of chromosome
5HS. PCR-based molecular markers were developed for saturation of this region based on previously mapped simple sequence repeat,
restriction fragment length polymorphism and Diversity Arrays Technology markers. In addition, defence gene homologue (DGH)
and wheat expressed sequence tags mapped in specific bins were used to develop new PCR markers. Seventeen PCR-based markers
were mapped to the short arm of chromosome 5H in 292 doubled haploid lines from a cross of Pompadour × Stirling, in which
seven markers were mapped within 5 cM of the APR gene. The closest linked marker was about 0.7 cM from the APR gene. The wheat
deletion bin map together with defence gene homologues was demonstrated to be an efficient tool for development of new molecular
markers associated with the disease resistance gene. Four DGH markers were associated with the APR gene. The new molecular
markers are a useful tool for marker-assisted selection of the APR gene and provided a better understanding of the molecular
mechanism for leaf rust resistance. 相似文献
8.
Osman A. Gutiérrez Arin F. Robinson Johnie N. Jenkins Jack C. McCarty Martin J. Wubben Franklin E. Callahan Robert L. Nichols 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2011,122(2):271-280
The identification of molecular markers that are closely linked to gene(s) in Gossypium barbadense L. accession GB713 that confer a high level of resistance to reniform nematode (RN), Rotylenchulus reniformis Linford & Oliveira, would be very useful in cotton breeding programs. Our objectives were to determine the inheritance of
RN resistance in the accession GB713, to identify SSR markers linked with RN resistance QTLs, and to map these linked markers
to specific chromosomes. We grew and scored plants for RN reproduction in the P1, P2, F1, F2, BC1P1, and BC1P2 generations from the cross of GB713 × Acala Nem-X. The generation means analysis using the six generations indicated that
one or more genes were involved in the RN resistance of GB713. The interspecific F2 population of 300 plants was genotyped with SSR molecular markers that covered most of the chromosomes of Upland cotton (G. hirsutum L.). Results showed two QTLs on chromosome 21 and one QTL on chromosome 18. One QTL on chromosome 21 was at map position
168.6 (LOD 28.0) flanked by SSR markers, BNL 1551_162 and GH 132_199 at positions 154.2 and 177.3, respectively. A second
QTL on chromosome 21 was at map position 182.7 (LOD 24.6) flanked by SSR markers BNL 4011_155 and BNL 3279_106 at positions
180.6 and 184.5, respectively. Our chromosome 21 map had 61 SSR markers covering 219 cM. One QTL with smaller genetic effects
was localized to chromosome 18 at map position 39.6 (LOD 4.0) and flanked by SSR markers BNL 1721_178 and BNL 569_131 at positions
27.6 and 42.9, respectively. The two QTLs on chromosome 21 had significant additive and dominance effects, which were about
equal for each QTL. The QTL on chromosome 18 showed larger additive than dominance effects. Following the precedent set by
the naming of the G. longicalyx Hutchinson & Lee and G. aridum [(Rose & Standley) Skovsted] sources of resistance, we suggest the usage of Ren
barb1
and Ren
barb2
to designate these QTLs on chromosome 21 and Ren
barb3
on chromosome 18. 相似文献
9.
Construction of a comprehensive PCR-based marker linkage map and QTL mapping for fiber quality traits in upland cotton (Gossypium hirsutum L.) 总被引:1,自引:0,他引:1
Zheng-Sheng Zhang Mei-Chun Hu Jian Zhang Da-Jun Liu Jing Zheng Ke Zhang Wei Wang Qun Wan 《Molecular breeding : new strategies in plant improvement》2009,24(1):49-61
To facilitate marker assisted selection, there is an urgent need to construct a saturated genetic map of upland cotton (Gossypium hirsutum L.). Four types of markers including SSR, SRAP, morphological marker, and intron targeted intron–exon splice junction (IT-ISJ)
marker were used to construct a linkage map with 270 F2:7 recombinant inbred lines derived from an upland cotton cross (T586 × Yumian 1). A total of 7,508 SSR, 740 IT-ISJ and 384
SRAP primer pairs/combinations were used to screen for polymorphism between the two mapping parents, and the average polymorphisms
of three types of molecular markers represented 6.8, 6.6 and 7.0%, respectively. The polymorphic primer pairs/combinations
and morphological markers were used to genotype 270 recombinant inbred lines, and a map including 604 loci (509 SSR, 58 IT-ISJ,
29 SRAP and 8 morphological loci) and 60 linkage groups was constructed. The map spanned 3,140.9 cM with an average interval
of 5.2 cM between two markers, approximately accounting for 70.6% of the cotton genome. Fifty-four of 60 linkage groups were
ordered into 26 chromosomes. Multiple QTL mapping was used to identify QTL for fiber quality traits in five environments,
and thirteen QTL were detected. These QTL included four for fiber length (FL), two for fiber strength (FS), two for fiber fineness (FF), three for fiber length uniformity (FU), and two for fiber elongation (FE), respectively. Each QTL explained between 7.4 and 43.1% of phenotypic variance. Five out of thirteen QTL (FL1 and FU1 on chromosome 6, FL2, FU2 and FF1 on chromosome7) were detected in five environments, and they explained more than 20% of the phenotypic variance. Eleven QTL
were distributed on A genome, while the other two on D genome. 相似文献
10.
QTL Mapping for Frond Length and Width in Laminaria japonica Aresch (Laminarales, Phaeophyta) Using AFLP and SSR Markers 总被引:1,自引:0,他引:1
Fuli Liu Zhanru Shao Haining Zhang Jidong Liu Xiuliang Wang Delin Duan 《Marine biotechnology (New York, N.Y.)》2010,12(4):386-394
In Laminaria japonica Aresch breeding practice, two quantitative traits, frond length (FL) and frond width (FW), are the most important phenotypic
selection index. In order to increase the breeding efficiency by integrating phenotypic selection and marker-assisted selection,
the first set of QTL controlling the two traits were determined in F2 family using amplified fragment length polymorphism (AFLP) and simple sequence repeat (SSR) markers. Two prominent L. japonicas inbred lines, one with “broad and thin blade” characteristics and another with “long and narrow blade” characteristics, were
applied in the hybridization to yield the F2 mapping population with 92 individuals. A total of 287 AFLP markers and 11 SSR markers were used to construct a L. japonica genetic map. The yielded map was consisted of 28 linkage groups (LG) named LG1 to LG28, spanning 1,811.1 cM with an average
interval of 6.7 cM and covering the 82.8% of the estimated genome 2,186.7 cM. While three genome-wide significant QTL were
detected on LG1 (two QTL) and LG4 for “FL,” explaining in total 42.36% of the phenotypic variance, two QTL were identified
on LG3 and LG5 for the trait “FW,” accounting for the total of 36.39% of the phenotypic variance. The gene action of these
QTL was additive and partially dominant. The yielded linkage map and the detected QTL can provide a tool for further genetic
analysis of two traits and be potential for maker-assisted selection in L. japonica breeding. 相似文献
11.
Fukino N Ohara T Monforte AJ Sugiyama M Sakata Y Kunihisa M Matsumoto S 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2008,118(1):165-175
Powdery mildew caused by Podosphaera xanthii is an important foliar disease in melon. To find molecular markers for marker-assisted selection, we constructed a genetic
linkage map of melon based on a population of 93 recombinant inbred lines derived from crosses between highly resistant AR
5 and susceptible ‘Earl’s Favourite (Harukei 3)’. The map spans 877 cM and consists of 167 markers, comprising 157 simple
sequence repeats (SSRs), 7 sequence characterized amplified region/cleavage amplified polymorphic sequence markers and 3 phenotypic
markers segregating into 20 linkage groups. Among them, 37 SSRs and 6 other markers were common to previous maps. Quantitative
trait locus (QTL) analysis identified two loci for resistance to powdery mildew. The effects of these QTLs varied depending
on strain and plant stage. The percentage of phenotypic variance explained for resistance to the pxA strain was similar between
QTLs (R
2 = 22–28%). For resistance to pxB strain, the QTL on linkage group (LG) XII was responsible for much more of the variance
(41–46%) than that on LG IIA (12–13%). The QTL on LG IIA was located between two SSR markers. Using an independent population,
we demonstrated the effectiveness of these markers. This is the first report of universal and effective markers linked to
a gene for powdery mildew resistance in melon. 相似文献
12.
Giancola S Marhadour S Desloire S Clouet V Falentin-Guyomarc'h H Laloui W Falentin C Pelletier G Renard M Bendahmane A Delourme R Budar F 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2003,107(8):1442-1451
The radish Rfo gene restores male fertility in radish or rapeseed plants carrying Ogura cytoplasmic male-sterility. This system was first discovered in radish and was transferred to rapeseed for the production of F1 hybrid seeds. We aimed to identify the region of the Arabidopsis genome syntenic to the Rfo locus and to characterize the radish introgression in restored rapeseed. We used two methods: amplified consensus genetic markers (ACGMs) in restored rapeseed plants and construction of a precise genetic map around the Rfo gene in a segregating radish population. The use of ACGMs made it possible to detect radish orthologs of Arabidopsis genes in the restored rapeseed genome. We identified radish genes, linked to Rfo in rapeseed and whose orthologs in Arabidopsis are carried by chromosomes 1, 4 and 5. This indicates several breaks in colinearity between radish and Arabidopsis genomes in this region. We determined the positions of markers relative to each other and to the Rfo gene, using the progeny of a rapeseed plant with unstable meiotic transmission of the radish introgression. This enabled us to produce a schematic diagram of the radish introgression in rapeseed. Markers which could be mapped both on radish and restored rapeseed indicate that at least 50 cM of the radish genome is integrated in restored rapeseed. Using markers closely linked to the Rfo gene in rapeseed and radish, we identified a contig spanning six bacterial artificial chromosome (BAC) clones on Arabidopsis chromosome 1, which is likely to carry the orthologous Rfo gene.Electronic Supplementary Material Supplementary material is available in the online version of this article at Communicated by H. C. BeckerS. Giancola and S. Marhadour contributed equally to this work 相似文献
13.
Peterka H Budahn H Schrader O Ahne R Schütze W 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2004,109(1):30-41
In rape (Brassica napus), no resistance to the beet cyst nematode (BCN) Heterodera schachtii is available. This study was carried out to determine the specific chromosome(s) of resistant radish (Raphanus sativus) carrying the gene(s) for nematode resistance as a prequisite to convert rape from a host into a trap crop for this pest. A Raphanobrassica progeny of 25 plants was analyzed which segregated for all nine chromosomes of the Raphanus genome in a genetic background of synthetic rape. The number of radish chromosomes was determined by fluorescence in situ hybridization, using the Raphanus-specific DNA probe pURsN; and their type was identified by chromosome-specific randomly amplified polymorphic DNA markers. Five different multiple rape–radish chromosome additions (comprising the whole set of nine radish chromosomes, a–i) were selected and crossed to rape. For each cross-progeny, the number of cysts on plant roots was counted 42 days after inoculation with a L2 larvae suspension. Simultaneously, the plants were characterized for the presence or absence of individual radish chromosomes, using sets of chromosome-specific markers. Thus, the effect of each radish chromosome on cyst number was tested. Chromosome d had a major resistance effect, whereas the presence/absence of the other radish chromosomes had nearly no influence on cyst number. Plants with added chromosome d showed a resistance level comparable with that of the radish donor parent. The analysis in the cross to rape of a plant monosomic only for chromosome d confirmed the strong effect of this chromosome on nematode resistance. A further experiment comprising seven crosses using winter rape breeding lines and monosomic addition line d as pollen parent provided the same results on a broader genetic basis. In each case, the added chromosome d in a single dosage caused nearly the full resistance of the radish donor. Resistance was independent of the glucosinolate content in the roots. The possibilities for stabilizing BCN resistance in rape and its use for other crops and nematodes are discussed.Communicated by C. Möllers 相似文献
14.
V. Lombard R. Delourme 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2001,103(4):491-507
A framework consensus map for rapeseed (Brassica napus L.) was constructed from the integration of three DH mapping populations derived from crosses between or within spring- and
winter-type parents. Several sources of genetic markers were used: isozymes, RFLPs, RAPDs, and AFLPs. A total of 992 different
markers were mapped to at least one population, of which 540 were included in the consensus map and 253 were common to at
least two populations. Markers were distributed over 19 linkage groups, thus reflecting the basic chromosome number of rapeseed
and covered 2,429 cM, which was in the mean confidence-interval estimates of genome length (2,127–2,480) cM. Markers were
evenly spaced on the entire genome even if, for several linkage groups, both RAPD and AFLP markers were not uniformly distributed.
In the population resulting from a cross between two spring lines, a higher recombination rate was observed and a translocation
was identified. The consensus approach allowed to map a larger number of markers, to obtain a near-complete coverage of the
rapeseed genome, to fill the number of gaps, and to consolidate the linkage groups of the individual maps.
Received: 19 July 2000 / Accepted: 31 October 2000 相似文献
15.
Xiuli Zhang Xiaorong Shen Yuanfeng Hao Jinjin Cai Herbert W. Ohm Lingrang Kong 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2011,122(2):263-270
The leaf rust resistance gene Lr19 and Fusarium head blight (FHB) resistance quantitative trait loci (QTL) derived from the wild wheatgrass Lophopyrum ponticum have been located on chromosome 7E. The main objectives of the present study were to develop a genetic map of chromosome
7E and map the two resistance loci using a population of 237 F7:8 recombinant inbred lines (RILs) derived from a cross between two Thatcher-L. ponticum substitution lines, K11463 (7el1(7D)) and K2620 (7el2(7D)). 532 G-SSR, E-SSR and STS markers from wheat chromosome group 7 were screened in the parent lines. Of these, 118 markers
were polymorphic, with a polymorphism frequency of 22.2%. A genetic map of L. ponticum chromosome 7E was constructed with 64 markers, covering 95.76 cM, with an average genetic distance of 1.47 cM between markers.
The major FHB resistance locus, temporarily assigned as FhbLoP, was mapped to the very distal region of the long arm of chromosome 7E within a 3.71 cM interval flanked by Xcfa2240 and Xswes19, which accounts for 30.46% of the phenotypic variance. Lr19 was bracketed by Xwmc273 and XBE404744, with a map distance of 1.54 and 1.43 cM from either side, respectively. The closely linked markers identified in this study
will be helpful for marker-assisted introgression of the L. ponticum-derived FhbLoP and Lr19 genes into elite cultivars of wheat, and the development of a genetic map will accelerate the map-based cloning of these
two genes. 相似文献
16.
Han OK Kaga A Isemura T Wang XW Tomooka N Vaughan DA 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2005,111(7):1278-1287
To make progress in genome analysis of azuki bean (Vigna angularis) a genetic linkage map was constructed from a backcross population of (V. nepalensis x V. angularis) x V.angularis consisting of 187 individuals. A total of 486 markers—205 simple sequence repeats (SSRs), 187 amplified fragment length polymorphisms
(AFLPs) and 94 restriction fragment length polymorphisms (RFLPs) —were mapped onto 11 linkage groups corresponding to the
haploid chromosome number of azuki bean. This map spans a total length of 832.1 cM with an average marker distance of 1.85 cM
and is the most saturated map for a Vigna species to date. In addition, RFLP markers from other legumes facilitated finding several orthologous linkage groups based
on previously published RFLP linkage maps. Most SSR primers that have been developed from SSR-enriched libraries detected
a single locus. The SSR loci identified are distributed throughout the azuki bean genome. This moderately dense linkage map
equipped with many SSR markers will be useful for mapping a range of useful traits such as those related to domestication
and stress resistance. The mapping population will be used to develop advanced backcross lines for high resolution QTL mapping
of these traits.
O.K. Han, A. Kaga, T. Isemura have contributed equally to this paper. 相似文献
17.
Akito Kamei Masato Tsuro Nakao Kubo Takeshi Hayashi Ning Wang Tatsuhito Fujimura Masashi Hirai 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2010,120(5):1021-1027
A QTL analysis for clubroot resistance (CR) of radish was performed using an F2 population derived from a crossing of a CR Japanese radish and a clubroot-susceptible (CS) Chinese radish. F3 plants obtained by selfing of F2 plants were used for the CR tests. The potted seedlings were inoculated and the symptom was evaluated 6 weeks thereafter.
The mean disease indexes of the F3 plants were used for the phenotype of the F2. The results of two CR tests were analyzed for the presence of QTL. A linkage map was constructed using AFLP and SSR markers;
it spanned 554 cM and contained 18 linkage groups. A CR locus was observed in the top region of linkage group 1 in two tests.
Therefore, the present results suggest that a large part of radish CR is controlled by a single gene or closely linked genes
in this radish population, although minor effects of other genomic areas cannot be ruled out. The CR locus was named Crs1. Markers linked to Crs1 showed sequence homology to the genomic region of the top of chromosome 3 of Arabidopsis, as in the case of Crr3, a CR locus in Brassica rapa. These markers should be useful for breeding CR cultivars of radish. As Japanese radishes are known to be highly resistant
or immune to clubroot, these markers may also be useful in the introgression of this CR gene to Brassica crops. 相似文献
18.
Genetic mapping and localization of a major QTL for seedling resistance to downy mildew in Chinese cabbage (Brassica rapa ssp. pekinensis) 总被引:1,自引:0,他引:1
Shuancang Yu Fenglan Zhang Renbo Yu Yanmin Zou Jiani Qi Xiuyun Zhao Yangjun Yu Deshuang Zhang Li Li 《Molecular breeding : new strategies in plant improvement》2009,23(4):573-590
Downy mildew caused by the fungus Peronospora parisitica is a serious threat to members of the Brassicaceae family. Annually, a substantial loss of yield is caused by the widespread
presence of this disease in warm and humid climates. The aim of this study was to localize the genetic factors affecting downy
mildew resistance in Chinese cabbage (Brassica rapa ssp. pekinensis). To achieve this goal, we improved a preexisting genetic map of a doubled-haploid population derived from a cross between
two diverse Chinese cabbage lines, 91-112 and T12-19, via microspore culture. Microsatellite simple sequence repeat (SSR)
markers, isozyme markers, sequence-related amplified polymorphism markers, sequence-characterized amplified region markers
and sequence-tagged-site markers were integrated into the previously published map to construct a composite Chinese cabbage
map. In this way, the identities of linkage groups corresponding to the Brassica A genome reference map were established. The new map contains 519 markers and covers a total length of 1,070 cM, with an
average distance between markers of 2.06 cM. All markers were designated as A1–A10 through alignment and orientation using
55 markers anchored to previously published B. rapa or B. napus reference maps. Of the 89 SSR markers mapped, 15 were newly developed from express sequence tags in Genbank. The phenotypic
assay indicated that a single major gene controls seedling resistance to downy mildew, and that a major QTL was detected on
linkage group A8 by both interval and MQM mapping methods. The RAPD marker K14-1030 and isozyme marker PGM flanked this major
QTL in a region spanning 2.9 cM, and the SSR marker Ol12G04 was linked to this QTL by a distance of 4.36 cM. This study identified
a potential chromosomal segment and tightly linked markers for use in marker-assisted selection to improve downy mildew resistance
in Chinese cabbage. 相似文献
19.
Extent and structure of linkage disequilibrium in canola quality winter rapeseed (Brassica napus L.)
Wolfgang Ecke Rosemarie Clemens Nora Honsdorf Heiko C. Becker 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2010,120(5):921-931
Linkage disequilibrium was investigated in canola quality winter rapeseed to analyze (1) the prospects for whole-genome association
analyses and (2) the impact of the recent breeding history of rapeseed on linkage disequilibrium. A total of 845 mapped AFLP
markers with allele frequencies ≥0.1 were used for the analysis of linkage disequilibrium in a population of 85 canola quality
winter rapeseed genotypes. A low overall level of linkage disequilibrium was found with a mean r
2 of only 0.027 over all 356,590 possible marker pairs. At a significance threshold of P = 2.8 × 10−7, which was derived by a Bonferroni correction from a global α-level of 0.1, only 0.78% of the marker pairs were in significant
linkage disequilibrium. Among physically linked marker pairs, the level of linkage disequilibrium was about five times higher
with more than 10% of marker pairs in significant linkage disequilibrium. Linkage disequilibrium decayed rapidly with distance
between linked markers with high levels of linkage disequilibrium extending only for about 2 cM. Owing to the rapid decay
of linkage disequilibrium with distance association analyses in canola quality rapeseed will have a significantly higher resolution
than QTL analyses in segregating populations by interval mapping, but much larger number of markers will be necessary to cover
the whole genome. A major impact of the recent breeding history of rapeseed on linkage disequilibrium could not be observed. 相似文献
20.
R. M. Klein-Lankhorst E. M. J. Salentijn W. G. Dirkse M. Arens-de Reuver W. J. Stiekema 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1994,89(4):426-434
A YAC library was constructed from the Beta vulgaris fragment addition AN5-203b. This monosomic fragment addition harbors an approximate 12-Mbp fragment of B.patellaris chromosome 1 accomodating the Hs1
pat-1
conferring resistance to the beet cyst nematode (Heterodera schachtii). The YAC library consists of 20,000 YAC clones having an average size of 140 kb. Screening with organelle-specific probes showed that 12% of the clones contain chloroplast DNA while only 0.2% of the clones hybridizes with a mitochondrial specific probe. On the basis of a sugar beet haploid genome size of 750 Mbp this library represents 3.3 haploid genome equivalents. The addition fragment present in AN5-203b harbors a major satellite DNA cluster that is tightly linked to the Hs1
pat-1
locus. The cluster is located on a single 250-kb EcoRI restriction fragment and consists of an estimated 700–800 copies of a 159-bp core sequence, most of which are arranged in tandem. Using this core sequence as a probe, we were able to isolate 1 YAC clone from the library that contains the entire 250-kb satellite DNA cluster.Abbreviations
YAC
Yeast artificial chromosome
-
BCN
beet cyst nematode
-
RAPD
random amplified polymorphic DNA
-
RFLP
restriction fragment length polymorphism 相似文献