阳桃的组织培养与植株再生

1植物名称阳桃(Averrhoa carambola),别名杨桃、五敛子、三廉子. 2材料类别无菌胚乳. 3培养条件诱导愈伤组织培养基:(1)MS 2,4-D 2.0 mg·L-1(单位下同) 6-BA 0.2;(2)MS 2,4-D 3.0 6-BA 0.1.诱导分化培养基:(3)MS ZT 3.0 NAA 0.2:(4)MS ZT 1.0 NAA 0.2:(5)MS ZT 5.0 NAA 0.2.生根培养基:(6)1/2 MS IBA 0.2 IAA 0.1.     

广玉兰组织培养和植株再生

植物名称:广玉兰(Magnolia grandiflora),又名荷花玉兰。材料类别:春季将要萌发的顶芽。培养条件:培养基为(1)MS ZT2.0mg/L(单位下同) 环烷酸钠0.05;(2)MS 6-BA 2.0 环烷酸钠0.05;(3)MS ZT 2.0 环烷酸钠0.05 活性炭3g/L;(4)MS 6-BA 2.0 环烷酸钠0.05 活性炭3g/L;(5)改良MS(大量元素减去2/3,盐酸硫胺素增至0.6,其余不变) NAA0.5 IAA0.5 TA0.1     

匙叶芋芽的诱导和植株再生

植物名称:匙叶芋Spathiphyllum sp.材料类别:根茎。培养条件:基本培养基为MS。诱导芽培养基,附加激素:(1) BA 2.0 mg/L(单位下同)+KT1.0,(2) KT0.5+NAA 0.2,(3) BA 0.5+IAA 1.0。芽增殖培养基,附加激素BA 1.0+IAA 0.1。生根培养基,MS减半,蔗糖2％,琼脂0.7％。培养温度25±2℃,每天光照10～12小时,光照强度2000 lx。     

菜豆的组织培养和植株再生

植物名称:菜豆(Phaseolus vulgaris)材料类别:上胚轴、幼茎。培养条件:基本培养基为MS。附加:(1) 6-BA 1.5～3.0mg/L(单位下同)+IAA 0.1:(2) 6-BA 20+2,4-D0.5;(3) IAA 0.01;(4) ZT2.0+IAA 0.2。以上培养基均加蔗糖3％,琼酯0.7％,pH 5.6,培养温度25±2℃,每天光照12小时,光照度     

黄山梅的组织培养和快速繁殖

1植物名称黄山梅(Kirengeshoma palmata Yatabe.). 2材料类别成熟种子. 3培养条件(1)种子萌发培养基:MS 6-BA 1.0mg·L-1(单位下同) IAA 0.5 活性炭(AC)0.1%;(2)增殖培养基:MS 6-BA 2.0 IAA 0.2;(3)壮苗培养基:MS 6-BA 0.5 IAA 0.5;(4)生根培养基:1/2MS 6-BA 0.1 NAA 1.0.各种培养基均附加3%蔗糖和0.6%琼脂,pH 6.0.培养温度为(25±2)℃,光强为30 μmol·m-2·s-1,光照时间12 h·d-1.     

黄花夹竹桃的组织培养与再生植株

植物名称:黄花夹竹桃(Thevetia Peruviana)别名:黄花状元竹,酒杯花。材料类别:幼叶、茎尖、带腋芽的茎段。培养条件:基本培养基为MS。(1)诱导培养基,MS附加BA1.0～2.0mg/l(单位下同)。NAA0.01～0.1。(2)分化培养基:MS附加BA0.3～0.5;KT0.1～0.2,NAA0.1～0.2。(3)生根培养基:1/2MS附加NAA0.1～0.5或IAA0.3～0.5。(1)、(2)培养基蔗糖用量为3％,(3)为1～1.5％;琼脂均为     

香瓜梨叶片的快速繁殖及植株再生

植物名称:香瓜梨(Solanum maracatum)。材料类别:叶片。培养条件:基本培养基为MS, 附加激素成分含量如下:(1)诱导愈伤组织培养基:2,4-D 0.3,0.5,1.0mg/L(单位下同);2,4-D0.5 KT(0.25,0.5)。(2)分化培养基:IAA0.1 KT(0.5,1.0,2.0,5.0);IAA0.1 BA(0.5,1.0,2.0,5.0);IAA0.1 2iP(0.5,1.0,2.0,5.0);IAA0.1 ZT     

滇大头茶的组织培养和微型繁殖

1植物名称滇大头茶(Gordonia yunnanensis). 2材料类别嫩梢的尖茎和茎节切段. 3培养条件(1)芽萌动和生长培养基:MS 6-BA 1.0mg·L-1(单位下同) IAA 0.5;(2)多芽体诱导培养基:MS 6-BA 3.0 IAA 0.5;(3)壮苗培养基:MS ZT1.0～2.0 IAA 0.5或MS 10%椰子汁(CM) IAA 0.5;(4)生根培养基:1/2ER IBA 0.5;(5)纸桥生根培养液:1/2ER,用IBA0.5～1.0g·L-1浸芽条基部20min,将芽条接种滤纸桥上,然后置弱光(200 lx)下培养.培养基添加3%(生根用2%)蔗糖和0.6%琼脂,pH5.6.培养温度25～27℃,夜间18～22℃,光照时间14h·d-1,光照度1 500 lx.     

假俭草的组织培养与植株再生

1植物名称假俭草(Eremochloa ophiuroides),又名百足草. 2材料类别成熟种子. 3培养条件(1)诱导愈伤组织培养基:MS 6-BA Q1mg·L-1(单位下同) 2,4-D 4.5.(2)继代培养基:MS 6-BA 0.1 2,4-D 4.0 Vc 5.0.(3)芽分化培养基:MS 6-BA 2.0 NAA1.0 CoCl25.0 TDZ 0.5.(4)生根培养基:1/2MS NAA 0.5 IAA 0.5 MET 0.5 活性炭0.1%.     

三尖杉的离体胚培养

1植物名称三尖杉(Cephalotaxus fortunei Hook.f.)。2材料类别离体胚。3培养条件芽诱导培养基:MS 6-BA2.0mg·L-1(单位下同) IAA1.0 NAA0.1;芽增殖培养基:MS 6-BA2.0 IAA1.0 NAA1.0;生根培养基:1/2MS IBA1.0 NAA0.5 KT0.2。以上各培养基     

Distribution of the core histones H2A.H2B.H3 and H4 during cell replication.

The distribution of newly synthesized core histones H2A, H2B, H3 and H4 relative to the DNA strand synthesized in the same generation has been examined in replicating Chinese Hamster ovary cells. Cells are grown for one generation in [14C]-lysine and thymidine, and then for one generation in [3H]-lysine and 5-bromodeoxyuridine (BrUdRib) and a further generation in unlabeled lysine and thymidine. This protocol produces equal amounts of unifilarly substituted and unsubstituted DNA. Monomer nucleosomes isolated from chromatin containing these two types of DNA can be distinguished by crosslinking with formaldehyde and banding to equilibrium in CsCl density gradients. The results indicate that the core histones are equally distributed between the two types of DNA. These findings are discussed in terms of current models for chromatin replication; they do not support any long term association of newly replicated histones with either the leading or lagging side of the replication fork.