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1.
N. Serizawa S. Nasuda F. Shi T. R. Endo S. Prodanovic I. Schubert G. Künzel 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2001,103(6-7):827-834
Chromosomal mutations in barley (Hordeum vulgare, 2n=2x=14, HH) chromosome 7H added to the common wheat (Triticum aestivum, 2n=6x=42, AABBDD) cultivar Chinese Spring were induced genetically by the gametocidal activity of certain alien chromosomes derived
from wild species of the genus Aegilops. The rearranged barley chromosomes were characterized by C-banding, FISH and GISH. Twenty two deletion or translocation chromosomes
in a hemizygous condition were selected for deletion mapping of 17 AFLP and 28 STS markers that are specific to 7H. Of the
22 breakpoints in chromosome 7H, seven involved the short arm (7HS), 12 the long arm (7HL) and three were in the centromeric
region. The seven 7HS breakpoints separated all four 7HS-specific AFLP markers and split the 21 STS markers into six groups.
One breakpoint occurred between two STS markers formerly occupying the same position in the genetic map. All seven 7HS breakpoints
were separated from each other by either the AFLP or STS markers. The 12 breakpoints in 7HL divided the 13 7HL-specific AFLP
markers into seven groups, and the seven STS markers into three groups. On the other hand, the 12 breakpoints in 7HL were
divided into six groups by the AFLP markers and into two groups by the STS markers. This deletion-based map was in accordance
with previously published genetic and physical maps using the same STS markers. The breakpoints, AFLP markers and STS markers
were arrayed in a consistent order.
Received: 5 February 2001 / Accepted: 19 February 2001 相似文献
2.
D. A. Laurie N. Pratchett K. M. Devos I. J. Leitch M. D. Gale 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1993,87(1-2):177-183
The 5S rDNA locus on the long arm of barley chromosome 2(2H) was genetically mapped in two crosses in relation to 30 other RFLP loci. Comparison of the genetic maps with the previously published physical position of the 5S rDNA, determined by in-situ hybridization, showed that there was a marked discrepancy between physical and genetic distance in both crosses, with recombination being less frequent in the proximal part of the arm. Pooled information from the present study and other published genetic maps showed that at least 26 of the 44 (59%) RFLPs that have been mapped on 2(2H)L lie distal to the 5S rDNA locus even though this region is only 27% of the physical length of the arm. The distribution of RFLP markers is significantly different from expected (P < 0.01), implying that the low-copy sequences used for RFLP analysis occur more frequently in distal regions of the arm and, or, that sequences in distal regions are more polymorphic. 相似文献
3.
W. Michalek M. Kleine H. Dargatz G. Wenzel A. Jahoor 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1997,95(3):369-374
The hordeins are the major class of storage proteins in barley and are encoded by multigene families. Two YAC-clones specific
for the C-hordein-coding Hor1-locus of barley (Hordeum vulgare L.) were selected. The clones were constructed with DNA from the cultivars ‘Franka’ and ‘Hockey’ and have insert sizes of
330 kb and 350 kb, respectively. Performing partial digestions and hybridizations with vector-specific probes, a restriction
analysis was conducted using restriction enzymes with a 8-bp recognition sequence. Both clones cover the complete region of
the Hor1-locus, but exhibit a different pattern of restriction sites reflecting the polymorphic nature of the locus on the scale of
long-range restriction mapping. The maximal extent of the regions homologous to the Hor1-specific probe, pBSC5, was 105 kb in the ‘Hockey’-derived YAC and 190 kb in the yeast artificial chromosome constructed with
‘Franka’-DNA. Furthermore the high degree of instability observed with the Hor1-specific YAC-clones is discussed in conjunction with the structure of the Hor1-locus.
Received: 19 December 1996 / Accepted: 31 January 1997 相似文献
4.
M. L. Wang M. D. Atkinson C. N. Chinoy K. M. Devos M. D. Gale 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1992,84(3-4):339-344
Summary A genetic map of barley chromosome 5 (1H) was constructed using DNA markers. Seventeen loci were mapped to 15 locations, and these included the known-function loci (in order from the most distal on the long arm) XAdh (alcohol dehydrogenase), XLec (homologous to wheat germ agglutinin), XHor3 (D-hordein), XPpdk (pyruvate orthophosphate dikinase), centromere, XIcal (chymotrypsin inhibitor), and 6 loci in the B- and C-hordein cluster towards the end of the short arm. The gene order on the barley map agreed closely with that of chromosome 1 of rye. Intervarietal comparisons showed that single-copy cDNA and genomic DNA probes revealed about twice the level of RFLPs found in wheat. 相似文献
5.
Y. Weng N. A. Tuleen G. E. Hart 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2000,100(3-4):519-527
Extended physical maps of chromosomes 6A, 6B and 6D of common wheat (Triticum aestivum L. em Thell., 2n=6x=42, AABBDD) were constructed with 107 DNA clones and 45 homoeologous group-6 deletion lines. Two-hundred and ten RFLP loci
were mapped, including three orthologous loci with each of 34 clones, two orthologous loci with each of 31 clones, one locus
with 40 clones, two paralogous loci with one clone, and four loci, including three orthologs and one paralog, with one clone.
Fifty five, 74 and 81 loci were mapped in 6A, 6B and 6D, respectively. The linear orders of the mapped orthologous loci in
6A, 6B and 6D appear to be identical and 65 loci were placed on a group-6 consensus physical map. Comparison of the consensus
physical map with eight linkage maps of homoeologous group-6 chromosomes from six Triticeaespecies disclosed that the linear
orders of the loci on the maps are largely, if not entirely, conserved. The relative distributions of loci on the physical
and linkage maps differ markedly, however. On most of the linkage maps, the loci are either distributed relatively evenly
or clustered around the centromere. In contrast, approximately 90% of the loci on the three physical maps are located either
in the distal one-half or the distal two-thirds of the six chromosome arms and most of the loci are clustered in two or three
segments in each chromosome.
Received: 19 April 1999 / Accepted: 28 July 1999 相似文献
6.
K. Sato T. Inukai P. M. Hayes 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2001,102(6-7):916-920
Barley is compatible with the rice blast pathogen (Pyricularia oryzae Cav.). Fiftyfour barley cultivars of diverse geographic origin and pedigree were inoculated with three isolates of the rice
blast pathogen. All barley genotypes showed blast disease symptoms when inoculated at the seedling stage with each of the
three isolates. However, one genotype showed quantitative resistance to all three isolates and three genotypes showed quantitative
resistance to one or two of the isolates. By inoculating a set of doubled-haploid lines derived from the cross ’Harrington’
(susceptible) and ’TR306’ (resistant) with isolate Ken 54–20, we mapped quantitative trait loci (QTLs) determining seedling
stage blast resistance. At all QTLs, TR306 contributed the resistance alleles. The four QTLs, when considered jointly, explained
43.6% of the phenotypic variation in blast symptom expression. A comparison of the blast resistance QTLs with other disease
resistance QTLs reported in this population revealed a region on chromosome 4 (4H) with multiple disease resistance loci.
It will be useful to capitalize on the syntenic relationship of rice and barley and to integrate information on species-specific
resistance genes with information on the reaction of the two species to the same pathogen.
Received: 7 January 2000 / Accepted: 22 September 2000 相似文献
7.
P. A. Butcher G. F. Moran R. Bell 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2000,100(3-4):576-583
Random genomic probes were used to assess levels of restriction fragment length polymorphism (RFLP) in two 2-generation outbred
pedigrees of Acacia mangium Willd. Probes were evaluated for their ability to detect polymorphic loci in each pedigree and to determine the relative
efficiency of different restriction enzymes in revealing polymorphisms. Sixty two percent of the probes which detected single-
or low-copy number sequences revealed polymorphisms with at least one restriction enyzme. HpaII was the most efficient in detecting polymorphism among first-generation individuals. The recognition sequence of HpaII contains a CpG dimer, suggesting that cytosines in the CpG sequence may be hotspots for mutation in plant genomes, as previously
reported in bacterial and mammalian genomes. Mendelian inheritance of 230 loci was demonstrated based on single-locus segregation
in second-generation individuals. Less than 5% of loci showed evidence of segregation distortion. The proportion of fully
informative loci (15%) was lower than previously reported in eucalypts reflecting the lower level of genetic diversity in
A. mangium. The RFLP probes are suitable for the construction of a high-density genetic linkage map in A. mangium. Cross-hybridisation of the A.mangium RFLPs to DNA from species representing the three subgenera of the genus Acacia indicates that these markers could be used in breeding programs of other diploid acacias, for comparative studies of genome
organisation, and for phylogenetic studies.
Received: 5. June 1999 / Accepted: 30 July 1999 相似文献
8.
The tomato high-pigment (hp) locus maps to chromosome 2 and influences plastome copy number and fruit quality 总被引:2,自引:0,他引:2
H. C. Yen B. A. Shelton L. R. Howard S. Lee J. Vrebalov J. J. Giovannoni 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1997,95(7):1069-1079
The tomato (Lycopersicon esculentum) high-pigment (hp) locus was originally described as having enhanced fruit-quality characteristics and has also been shown to regulate responses
to light during growth and development. Specifically, the hp phenotype suggests that the normal HP gene-product serves as a negative regulator of light signal-transduction, as has been proposed for many of the previously
described Arabidopsis thaliana photomorphogenic mutants. Consequently, hp represents a tool for both genetic dissection of light signal-transduction and manipulation of fruit quality in tomato. As
a first step toward isolation of the HP gene, the hp locus was mapped to tomato chromosome 2, adjacent to the 45s rDNA locus, using DNA markers and an interspecific cross of L. esculentum×L. cheesmannii. We have simultaneously identified DNA markers which may be useful for gene isolation and marker-assisted selection. We have
additionally extended characterization of the hp phenotype to demonstrate increased sucrose and flavonoid accumulation in ripe hp/hp fruit. Analysis of plastid DNA copy number relative to genomic DNA content indicates that the hp locus regulates plastome DNA concentration, and possibly plastid number, in response to light.
Received: 26 June 1997 / Accepted: 22 July 1997 相似文献
9.
Comparative mapping of the wheat chromosome 5A Vrn-A1 region with rice and its relationship to QTL for flowering time 总被引:3,自引:0,他引:3
R. N. Sarma B. S. Gill T. Sasaki G. Galiba J. Sutka D. A. Laurie J. W. Snape 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1998,97(1-2):103-109
The vernalization gene Vrn-A1 on chromosome 5A is the predominant gene determining the spring/winter habit difference in bread wheat. Vrn-A1 was physically mapped using a set of deletion lines which located it to the region of chromosome 5A flanked by deletion breakpoints
0.68 and 0.78. This interval was shown to be homoeologous to a region of rice chromosome 3 that contains the flowering-time
QTL Hd-6, previously mapped in a Nipponbare×Kasalath cross, and FLTQ1, a novel QTL identified by analysis of 78 F3 families derived from a cross of ‘IR20’ב63–83’. Possible relationships between Vrn-A1 and rice QTL are discussed. Analysis of the chromosome 5A deletion lines showed evidence for a second, more proximal flowering-time
effect located between deletion breakpoints 0.56 and 0.64. The proximal part of chromosome 5A is homoeologous to rice chromosome
9, on which two QTL were detected in the ‘IR20ב63–83’ cross. The possible relationship between these effects is also discussed.
Received: 23 December 1997 / Accepted: 12 January 1998 相似文献
10.
S. P. Jefferies A. R. Barr A. Karakousis J. M. Kretschmer S. Manning K. J. Chalmers J. C. Nelson A. K. M. R. Islam P. Langridge 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1999,98(8):1293-1303
Boron toxicity has been recognised as an important problem limiting production in the low-rainfall regions of southern Australia,
West Asia and North Africa. Genetic variation for boron toxicity tolerance in barley has been characterised but the mode of
inheritance and the location of genes controlling tolerance were not previously known. A population of 150 doubled-haploid
lines from a cross between a boron toxicity tolerant Algerian landrace, Sahara 3771, and the intolerant Australian cultivar
Clipper was screened in four tolerance assays. An RFLP linkage map of the Clipper×Sahara population was used to identify chromosomal
regions associated with boron tolerance in barley. Interval regression-mapping allowed the detection of four chromosomal regions
involved in the boron tolerance traits measured. A region on chromosome 2H was associated with leaf-symptom expression, a
region on chromosome 3H was associated with a reduction of the affect of boron toxicity on root growth suppression, a region
on chromosome 6H was associated with reduced boron uptake, and a region on chromosome 4H was also associated with the control
of boron uptake as well as being associated with root-length response, dry matter production and symptom expression. The benefits
and potential of marker-assisted selection for boron toxicity tolerance are discussed.
Received: 18 December 1997 / Accepted: 28 November 1998 相似文献
11.
A. Börner V. Korzun S. Malyshev V. Ivandic A. Graner 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1999,99(3-4):670-675
The two recessive dwarfing mutants gai (GA-ins) and gal (GA-less), differing in their response to exogenously applied gibberellic acid (GA3), were mapped in the centromere region and on the long arm, respectively, of the barley chromosome 2H. The gene gai, which determines reduced plant height and GA insensitivity pleiotropically, was found to co-segregate with the two RFLP
markers Xmwg2058 and Xmwg2287. Both markers are known to map close to the centromere. The GA-sensitive dwarfing gene gal was found to be linked to the three co-segregating RFLP markers Xmwg581, Xmwg882 and Xmwg2212 (proximal) and XksuG5 (distal) by 3.6 and 9.5. cM, respectively. The distance between the two mutant loci was estimated to be about 55 cM. Homoeologous
relationships between the dwarfing genes within the Triticeae are discussed.
Received: 11 December 1998 / Accepted: 11 February 1999 相似文献
12.
RFLP- and physical mapping of resistance gene homologues in rice (O. sativa) and Barley (H. vulgare)
D. Leister J. Kurth D. A. Laurie M. Yano T. Sasaki A. Graner P. Schulze-Lefert 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1999,98(3-4):509-520
The deduced peptide sequences of 25 gene fragments of NBS-LRR resistance (R) gene homologues from rice and barley and of characterized R genes were compared, revealing a string of six conserved motifs. Mapping of the R-gene candidates in rice showed linkage to genes conferring race-specific resistance to rice blast (Pi-k, Pi-f and Pi-1) and bacterial blight disease (Xa-1, Xa-3 and Xa-4), in barley to powdery mildew (Mla) and the rust fungus (Rpg1). In rice four mixed clusters were detected, each harboring at least two highly dissimilar NBS-LRR genes. A YAC-contig was
established for one of these mixed clusters. YAC fragmentation experiments revealed the presence of at least five NBS-LRR
genes within 200 kb in head-to-tail orientation.
Received: 24 July 1998 / Accepted: 14 August 1998 相似文献
13.
High-resolution linkage analysis and physical characterization of the EIX-responding locus in tomato
M. Ron R. Kantety G. B. Martin N. Avidan Y. Eshed D. Zamir A. Avni 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2000,100(2):184-189
An ethylene-inducing xylanase (EIX) from Tricohoderma viride is a potent elicitor of ethylene biosynthesis, localized cell death and other defense responses in specific cultivars of
tobacco (Nicotiana
tabacum) and tomato (Lycopersicon esculentum). Wild species of tomato, such as Lycopersicon cheesmanii and Lycopersicon pennellii, do not respond to EIX treatment. The F1 progeny of a L. esculentum×L. cheesmanii and a L. esculentum×L. pennellii cross responded to EIX treatment with an increase in ethylene biosynthesis and the induction of localized cell death. The
F2 progeny of the above mentioned crosses segregated 3:1 (responding:non-responding). We mapped the EIX-responding locus (Eix) to the short arm of chromosome 7 using a population of introgression lines (ILs), containing small RFLP-defined chromosome
segments of L. pennellii introgressed into L. esculentum. RFLP analysis of 990 F2 plants that segregated for the introgressed segment mapped the Eix locus 0.1 cM and 0.9 cM from the flanking markers TG61 and TG131, respectively. Using the marker TG61 we isolated a yeast
artificial chromosome (YAC) clone that carries 300-kb DNA segments derived from the Eix region. By mapping the ends of this YAC clone we show that it spans the Eix locus. Thus, positional cloning of the Eix locus appears feasible.
Received: 20 March 1999 / Accepted: 30 April 1999 相似文献
14.
I. Karsai K. Mészáros P. M. Hayes Z. Bedő 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1997,94(5):612-618
Heading-date in cereals is the final result of a number of interacting characters that include vernalization requirement,
photoperiod sensitivity, and earliness per se. Progress in developing adapted varieties may be achieved by determining the chromosomal locations of genes controlling these
characters. Nineteen doubled-haploid (DH) lines from the Dicktoo×Morex mapping population were phenotyped in controlled- environment photoperiod experiments to determine the role of two previously
detected QTLs on the developmental patterns of barley. The QTLs are hypothesised to represent the effects of the Ppd and Sh2 loci on chromosomes 2 (2H) and 7 (5H), respectively. Alleles at the Ppd locus were found to be vary in response to photoperiod duration. Vernalization had some effect on alleles at both loci. The
presence of early and late- flowering transgressive segregants in this mapping population can be explained by interactions
between the Ppd and Sh2 loci. The Ppd and Sh2 loci are hypothesised to be homoeologous with the Ppd and Vrn1 loci of wheat.
Received: 1 August 1996 / Accepted: 15 November 1996 相似文献
15.
K. J. Williams A. Lichon P. Gianquitto J. M. Kretschmer A. Karakousis S. Manning P. Langridge H. Wallwork 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1999,99(1-2):323-327
Spot form of net blotch (SFNB) (Pyrenophora teres f maculata) is an economically damaging foliar disease of barley in many of the world’s cereal growing areas. The development of SFNB-resistant
cultivars may be accelerated through the use of molecular markers. A screen for SFNB resistance in 96 lines identified four
new sources of resistance, including a feed variety, ‘Galleon’, for which a fully mapped doubled haploid population was available.
Segregation data indicated SFNB resistance was conferred by a single gene in the ‘Galleon’בHaruna Nijo’ cross, positioned
on the long arm of chromosome 7H. This gene is designated Rpt4 and is flanked by the RFLP loci Xpsr117(D) and Xcdo673 at distances of 6.9 cM and 25.9 cM, respectively. The marker Xpsr117(D) was validated using another population segregating for Rpt4, correctly predicting SFNB resistance with more than 90% accuracy.
Received: 24 September 1998 / Accepted: 19 December 1998 相似文献
16.
Variants of the pulsed-field gel electrophoresis technique were used in conjunction with two-dimensional DNA gel electrophoresis (2-DDGE) to determine the ratio of physical to genetic distance in two genetically defined intervals on barley chromosome 1H.2-DDGE analysis demonstrated that two loci that define a 0.3 cM interval, as determined by hybridization with BCD249, reside on a single 450-kbMluI fragment. This result indicates a maximum ratio of physical to genetic distance in this interval of 1500 kb/cM as compared to 3.7–4.2 Mb/cM for the barley genome as a whole. High molecular weight (HMW) DNA restricted withNotI and probed sequentially with MWG068 and BCD249 yield diffuse bands at approximately 2.8 Mb and 3.0 Mb in the C.I. 16151 and C.I. 16155 parental lines, respectively. These results suggest the maximum ratio of physical to genetic distance in the interval defined by these probes is 7.8 Mb/cM. unique HMW DNA restriction fragment length polymorphisms (RFLP) were attributed to the presence of recombination breakpoints. Data from the recombination breakpoint analysis were used to estimate a ratio of physical to genetic distance of 2.5 Mb/cM in theXbcd249.2-Xmwg068 interval and 0.465 Mb/cM in theXbcd249.1-Xbcd249.2 interval. Both physical linkage and recombination breakpoint analysis indicate theXbcd249.1-Xbcd249.2 interval is approximately five-fold smaller, physically, than theXbcd249.2-Xmwg068 interval.Names are necessary to report factually on available data; however the USDA neither guarantees nor warrants the standard of the product and the use of the name by USDA implies no approval of the product to the exclusion of others that may also be suitable 相似文献
17.
S. L. Fox E. N. Jellen S. F. Kianian H. W. Rines R. L. Phillips 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2001,102(2-3):320-326
The availability of molecular genetic maps in oat (Avena spp.) and improved identification of chromosomes by C-banding are two recent developments that have made locating linkage
groups to chromosomes possible in cultivated hexaploid oat, 2n=6x=42. Monosomic series derived from Avena byzantina C. Koch cv Kanota and from Avena sativa L. cv Sun II were used as maternal plants in crosses with the parents, Kanota-1 and Ogle-C, of the oat RFLP mapping population.
Monosomic F1 plants were identified by root-tip cell chromosome counts. For marker analysis, DNAs of eight F2 plants from a monosomic F1 were combined to provide a larger source of DNA that mimicked that of the monosomic F1 plant. Absence of maternal alleles in monosomic F1s served to associate linkage groups with individual chromosomes. Twenty two linkage groups were associated with 16 chromosomes.
In seven instances, linkage groups that were independent of each other in recombination analyses were associated with the
same chromosome. Five linkage groups were shown to be associated with translocation differences among oat lines. Additionally,
the results better-characterized the oat monosomic series through the detection of duplicates and translocation differences
among the various monosomic lines. The F1 monosomic series represents a powerful cytogenetic tool with the potential to greatly improve understanding of the oat genome.
Received: 24 April 2000 / Accepted: 10 May 2000 相似文献
18.
H. Salvo-Garrido D. A. Laurie B. Jaffé J. W. Snape 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2001,103(6-7):869-880
This paper describes the first extensive genetic map of Hordeum bulbosum, the closest wild relative of cultivated barley. H. bulbosum is valuable for haploid production in barley breeding, and because of desirable agronomic characteristics, it also has potential
for trait introgression into barley. A H. bulbosum map will assist introgression and provide a basis for the identification of QTLs for crossability with barley and other potentially
useful genes. The present study used a population of 111 individuals from a PB1×PB11 cross to develop a genetic linkage map
of diploid H. bulbosum (2n=2x=14) based on barley, wheat and other ”anchor” cereal RFLP markers previously mapped in other species. Because of the cross-pollinating
and highly polymorphic nature of H. bulbosum, up to four alleles showed segregation at any one locus, and five different segregation types were found. This enabled maps
to be developed for the PB1 and PB11 parents, as well as a combined map. In total, 136 RFLP loci were mapped with a marker
coverage of 621 cM. The markers were generally colinear with barley but H. bulbosum had less recombination in the centromeric regions and similar or more in the distal regions. Cytological studies on pollen
mother cells at metaphase-I showed marked distal localization of chiasmata and a frequency consistent with the genetic map
length. This study showed that H. bulbosum was highly polymorphic, making it suitable for trait analysis and supplementing maps of barley.
Received: 20 November 2000 / Accepted: 5 January 2001 相似文献
19.
Cheng CH Chung MC Liu SM Chen SK Kao FY Lin SJ Hsiao SH Tseng IC Hsing YI Wu HP Chen CS Shaw JF Wu J Matsumoto T Sasaki T Chen HH Chow TY 《Molecular genetics and genomics : MGG》2005,274(4):337-345
A fine physical map of the rice (Oryza sativa spp. Japonica var. Nipponbare) chromosome 5 with bacterial artificial chromosome (BAC) and PI-derived artificial chromosome (PAC) clones
was constructed through integration of 280 sequenced BAC/PAC clones and 232 sequence tagged site/expressed sequence tag markers
with the use of fingerprinted contig data of the Nipponbare genome. This map consists of five contigs covering 99% of the
estimated chromosome size (30.08 Mb). The four physical gaps were estimated at 30 and 20 kb for gaps 1–3 and gap 4, respectively.
We have submitted 42.2-Mb sequences with 29.8 Mb of nonoverlapping sequences to public databases. BAC clones corresponding
to telomere and centromere regions were confirmed by BAC-fluorescence in situ hybridization (FISH) on a pachytene chromosome.
The genetically centromeric region at 54.6 cM was covered by a minimum tiling path spanning 2.1 Mb with no physical gaps.
The precise position of the centromere was revealed by using three overlapping BAC/PACs for ~150 kb. In addition, FISH results
revealed uneven chromatin condensation around the centromeric region at the pachytene stage. This map is of use for positional
cloning and further characterization of the rice functional genomics.
Electronic supplementary material Supplementary material is available in the online version of this article at
and is accessible for authorized users.
Chia-Hsiung Cheng and Mei-Chu Chung have equal contributions. 相似文献
20.
Polymorphism, distribution, and segregation of AFLP markers in a doubled haploid rice population 总被引:18,自引:0,他引:18
M. Maheswaran P. K. Subudhi S. Nandi J. C. Xu A. Parco D. C. Yang N. Huang 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1997,94(1):39-45
We exploited the newly developed amplified fragment length polymorphism (AFLP) technique to study the polymorphism, distribution
and inheritance of AFLP markers with a doubled haploid rice population derived from ‘IR64’/‘Azucena’. Using only 20 pairs
of primer combinations, we detected 945 AFLP bands of which 208 were polymorphic. All 208 AFLP markers were mapped and distributed
over all 12 chromosomes. When these were compared with RFLP markers already mapped in the population, we found the AFLP markers
to be highly polymorphic in rice and to follow Mendelian segregation. As linkage map of rice can be generated rapidly with
AFLP markers they will be very useful for marker-assisted backcrossing.
Received: 11 April 1996 / Accepted: 14 June 1996 相似文献