Antibody formation by cells isolated from donors of different ages after transfer to young rabbits and to X-irradiated adult rabbits

Antibody formation by spleen cells isolated from adult, non-immunized donors and mixed withBrucella suis antigenin vitro was compared after transfer to young rabbits and to adult recipients irradiated with a dose of 450 r. It was found that this dose did not produce complete inhibition and that the recipients still responded actively to the given antigen. Only young animals, therefore, are a suitable experimental model in which to study antibody formation by transferred isolated cells. Development of the antibody reaction in young rabbits was studied by transferring cells isolated from the spleen of animals aged 8–30 days and mixed with antigenin vitro. Antibodies were formed after the transfer of these cells to X-irradiated recipients, but by the active response of the latter to the antigen. Antibody formation by cells of donors of different ages can be determined after transfer to young animals. The youngest donors whose cells form antibodies after transfer are three-week-old rabbits, but demonstration of antibodies requires the transfer of quite a large number of cells. The results are discussed in relation to the experiments of other authors on the transfer of cells to young animals.     

Basophil-sensitizing antibody response to herpes simplex viruses in rabbits.

Antibodies to herpes simplex virus type 1 and type 2 were detected in the sera of rabbits by release of histamine from basophils sensitized in vitro with the sera. The time course of the appearance of the antibodies, the dose-response curve of the release of histamine in relation to antigen concentration, the sedimentation characteristics of the antibodies in sucrose gradients, and the ability to destroy the sensitizing capacity of the sera with heat suggest that the antibodies being assessed were of the IgE class. These antibodies were induced in animals injected intradermally, intramuscularly, and i.p. with live virus. The antibodies were detected 1 week after primary injection and a similar time course of antibody appearance was observed after a second or third injection. The same cross-reactivity between type 1 and type 2 virus observed with IgG antibody was also observed with IgE antibody.     

Antibody formation in young rabbits immunized with erythrocytes

Крольчат различного возраста иммунизировали взвесью чуживотных эритроцитов. У всех животных,—даже при иммунизации на первый день после рождения,—наблюдалось образование антител, которое можно было доказать гемагглютинацией уже на 5-й день после иммунизации. Возраст крольчат не оказывал влияния на скорость образования антител. Реакцию образования антител можно было подавить облучением крольчат 350 r за 48 час. до иммунизации.— Обсуждаются возможные механизмы этой реакции.     

Stimulation of antibody formation by bacterial lipopolysaccharide. The influence of repeated doses of lipopolysaccharide on antibody formation

1. (1)
   Липополисахарид стимулирует образование антител даже при 8-кратном введении в сочетании с частично очищенным дифтерийным анатоксином.     
   
   

Taxonomic identification of gram-negative nonfermenting microorganisms in environmental research

The scheme for the identification of Gram-negative nonfermenting microorganisms is proposed. The scheme comprises the most important key signs, such as the cytochrome oxidase reaction determined by the method of Gaby and Hadley, the oxidation/fermentation test, maltose oxidation and motility, as well as additional key signs, among them gelatinase activity, the oxidation of 10% lactose, nitratase activity with the liberation of free nitrogen, the utilization of the sources of carbon and energy (glucose and sodium acetate) in limited media containing ammonium salts and nitrates as the sources of nitrogen. Additional tests for the identification of nonfermenting microorganisms similar in their main key signs are also recommended.