Relationship Between Immune Cell Phenotypes and Pig Growth in a Commercial Farm

The objective of this study was to evaluate the relationship between the level and function of circulating immune cells with average daily gain, live and carcass measurements, feed intake, and feed conversion. Production performance was monitored throughout the pig's lifetime. Pigs were moved in weekly batches through the nursery and growing/finishing rooms at specific target weights. Animals were individually weighed at birth and at weaning, and then every two weeks while they were “on test” until they were “off test” and sent to the slaughterhouse. At six to seven weeks of age, the pigs were bled in the nursery. The percentage of immune cell subsets and lymphocyte proliferation was estimated using swine monoclonal antibodies and flow cytometric analysis. The predictive effect of the immune cell subset markers and lymphocyte proliferation on production traits was statistically analyzed. The results indicated that the proportion of several peripheral cell subsets, including CD16+, CD2+/CD16+, and CD8+ lymphocytes, appear to predict growth during the entire productive life of the pig. Larger percentages of lymphocytes expressing CD16+ CD2+/CD16+, and CD8+receptors in blood resulted in a reduction in average daily gain. In addition, high percentages of SLA-DQ+ cells were associated with better carcass weight and feed conversion. The CD16+, CD2+/CD16+, CD8+, and SLA-DQ± cell subsets appear to be important biomarkers involved with the inherent ability of the pig to efficiently grow and produce better carcass weight in representative commercial environments.     

Redistribution of natural killer (NK) cell frequency and NK cytotoxic activity in primary IgA nephropathy.

Recent findings have indicated an imbalance of immune responsiveness in primary IgA nephropathy (IgAN). Thus natural killer (NK) cell frequency and NK cytotoxicity were evaluated in fifteen IgAN patients. CD8+, CD11+, CD56+ and CD57+ lymphocyte percentages in IgAN individuals fell within normal values, while a significant decrease of CD16+ cells was observed in the same group of patients. In contrast, NK activity overlapped that seen in controls as assessed by an agarose-single cell cytotoxic assay. To further investigate the discrepancy between CD16+ cell level and NK cytotoxic activity in IgAN, the proportion of CD11+ CD57+, CD56+ CD16+ and CD57+ CD16+ lymphocytes was determined. In spite of the unaffected CD56+ CD16+ cell frequency, IgAN subjects exhibited a significant decrease of CD11+ CD57+ and CD57+ CD16+ lymphocyte percentages in comparison to controls. It is suggested that a redistribution of NK lymphocyte subsets occurs in IgAN. This may have an important role in the impairment of the immunoregulatory network.     

Distribution of ecto-5'-nucleotidase on subsets of human T and B lymphocytes as detected by indirect immunofluorescence using goat antibodies

Human T and B lymphocyte subsets were characterized for ecto-5'-nucleotidase (ecto-5'-NT) expression by two-color immunofluorescence by using polyclonal goat antibodies to 5'-NT and murine monoclonal antibodies to T and B cell subsets. Anti-5'-NT antibodies were prepared by immunizing a goat with purified human placental 5'-NT. Lymphocyte surface 5'-NT was detected with F(ab')2 fragments of immune goat IgG followed by biotinylated F(ab')2 rabbit anti-goat IgG and fluorescein isothiocyanate-avidin. Lymphocyte cell surface antigens were detected with phycoerythrin (PE)-conjugated anti-CD3, anti-CD4, anti-CD8, anti-CD16, and anti-CD19. HB-4, an antigen present on a major subset of human peripheral blood B cells, was detected with murine monoclonal anti-HB-4 and PE-anti-mouse-kappa. Analysis showed that ecto-5'-NT was expressed on 32 +/- 7% of CD3+, 19 +/- 6% of CD4+, and 50 +/- 21% of CD8+ T cells, but not on CD16+ lymphocytes. Ecto-5'-NT was also expressed on 81 +/- 8% of adult peripheral blood B cells as defined by PE-anti-CD19; HB-4 was expressed on 84 +/- 7% of CD19+ cells. The two populations of B cells were not identical, however, because HB-4 was co-expressed on only 79 +/- 18% of ecto-5'-NT+ B cells. Two-color immunofluorescent staining of T cells from a patient with congenital agammaglobulinemia and low T cell ecto-5'-NT activity revealed reduced percentages of ecto-5'-NT+ cells in his CD3+, CD4+, and CD8+ populations. Thus, reduced ecto-5'-NT activity by enzyme assay was paralleled by reduced numbers of 5'-NT molecules on the cell surface. Two-color immunofluorescent staining of B cells from a patient with hypogammaglobulinemia and low B cell ecto-5'-NT activity also revealed markedly reduced expression of 5'-NT. HB-4 expression was normal, however, suggesting that the patient's B cells were blocked in maturation subsequent to the acquisition of HB-4 but prior to that of ecto-5'-NT. These results demonstrate that anti-5'-NT antibodies will be valuable tools for analyzing ecto-5'-NT expression and lymphocyte maturation in patients with immuno-deficiency diseases.     

The clinical value of the changes of peripheral lymphocyte subsets absolute counts in patients with non-small cell lung cancer

IntroductionImmune function strongly influences the outcome of patients with non-small cell lung cancer (NSCLC). It's vital to understand the immune state of patients through detecting the percentage and number of lymphocyte subsets accurately, and helpful to evaluate conditions of prognosis and adjust treatment for patients.MethodsWe conducted a retrospective cohort study in First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin, China. The absolute counts and percentages of CD3+, CD3 + CD4+, CD3 + CD8+, B and NK cells were determined by single platform technologies. 172 patients received treatment including surgery or chemotherapy after surgery. The factors affecting disease progression were analyzed by Binary Logistic regression. Progression free survival (PFS) calculating survivals were with the method of Kaplan-Meier. The log-rank test and cox's proportional hazard regression (enter method) were used for univariable and multivariable analyses respectively.ResultsRelative to normal controls, patients with NSCLC at different stages showed decreased absolute lymphocyte count obviously, rather than lymphocyte percentages.Different treatments had unlike influence on the homeostasis of lymphocytes and the effects last for a long time. Logistic regression showed CD3 + CD4+ and CD3 + CD8+ could contribute to favorable prognosis. Multivariate analysis of prognostic factors of PFS showed CD3 + CD4+ cell was independent factor for predicting PFS.ConclusionsThe absolute count of CD3+, CD3 + CD4+, CD3 + CD8+, B and NK cells were better indication of the patient's immune state than percentages of each lymphocyte subsets. Immune function was impaired in patients with non-small cell lung. The high level of baseline absolute CD3 + CD4+ cells count contributed to longer progression free survival.Chinese Clinic Trial Registry number: ChiCTR-IOR-17014139; Registry date: 2017/12/25.     

不同临床类型乙型病毒感染者外周血T 细胞亚群与血清HBV DNA 载量 及HBeAg 滴度相关性分析

目的:探讨慢性乙型肝炎病毒(HBV)感染患者外周血T细胞亚群与血清HBV DNA载量及HbeAg滴度的关系。方法:选取103名HBV感染患者和20名健康者为研究对象。流式细胞术检测外周血T细胞亚群,聚合酶链式反应及酶免疫分析法分别检测血清HBV DNA载量及HbeAg滴度。结果:慢性乙型肝炎患者和慢性HBV携带者外周血CD3+T、CD4+T淋巴细胞亚群百分数低于健康对照组,结果有统计学意义(P<0.05或0.01;而CD8+T细胞亚群则呈现相反趋势,结果亦有统计学意义(P<0.05或0.01)。HBeAg阴性组中,HBVDNA水平与CD8+T细胞亚群百分数呈正相关(r=0.567,P<0.01),与CD4+/CD8+T细胞亚群百分数比值呈负相关(r=-0.601,P<0.01),而与CD3+T、CD4+T细胞亚群百分数无相关性。HBeAg阳性组中,HBV DNA水平及HbeAg滴度与CD3+T、CD4+T、CD8+T细胞百分数及CD4+/CD8+T细胞百分数均无相关性(P>0.05)。结论:不同临床类型的慢性乙型肝炎病毒感染患者外周血T细胞亚群存在不同程度细胞免疫功能降低和细胞免疫调节异常。HbeAg阴性的HBV感染患者,其血清HBV DNA水平与外周血T淋巴细胞免疫存在相关性。     

化疗在中晚期非小细胞肺癌患者中对淋巴细胞亚群影响分析

目的:探讨化疗在中晚期非小细胞肺癌患者中对淋巴细胞亚群的影响。方法:随机抽取本院收治的60例中晚期非小细胞肺癌患者编为实验组进行化疗,另选取同期体检的50例健康志愿者作为对照组。随访12月-15月,采用流式细胞仪技术分别对两组外周血淋巴细胞亚群进行检测计数。结果:两组间相比,实验组患者的CD3+、CD4+、NK细胞的数量以及CD4+/CD8+比值均低于对照组(P<0.05),而CD8+细胞的比例却高于对照组。化疗后CD3+、CD4+、CD4+/CD8+、NK均较化疗前升高(P<0.05),但CD8+不变(P>0.05)。结论:应用化疗治疗中晚期非小细胞肺癌,可明显改善患者的免疫功能。     

中药复方连黄对小鼠T淋巴细胞亚群CD4~+、CD8~+的影响

目的探讨中药复方连黄对小鼠T淋巴细胞亚群CD4+、CD8+的影响.方法 T淋巴细胞亚群测定采用单克降抗体直接免疫荧光技术,通过流式细胞仪测定.结果经统计学分析,与对照组比较,中药复方连黄能不同程度地使CD4+、CD4+/CD8+升高,而使CD8+下降.结果表明,中药复方连黄对细胞免疫功能具有调节作用.     

康莱特配伍吉非替尼治疗对晚期非小细胞肺癌患者免疫功能和生活质量的影响

目的:评估康莱特(KLT)配伍吉非替尼治疗对晚期非小细胞肺癌(NSCLC)的免疫功能和生活质量的影响。方法:选取我院2013年1月-2014年7月诊治的晚期NSCLC患者64例并随机分为两组各32例。对照组给予吉非替尼口服,试验组在对照组基础上加KLT静脉滴注。观察两组T淋巴细胞亚群CD3~+、CD4~+、CD8~+、CD4~+/CD8~+表达情况及自然杀伤(NK)细胞活性;采用生活质量量表(QLQ-CCC)和卡式功能状态量表(KPS)对生活质量及功能状态进行评估。结果:试验组T淋巴细胞亚群CD4~+、CD4~+/CD8~+表达率及NK细胞活性高于对照组(P0.05),且其治疗前后的差值低于对照组(P0.05);试验组躯体、心理、社会、QLQ-CCC总分及KPS得分均高于对照组,且其治疗前后的差值均高于对照组(P0.05)。结论:KLT配伍吉非替尼治疗晚期NSCLC可减少吉非替尼对免疫功能的损害,提高患者的生活质量。     

肝癌患者体液免疫和细胞免疫的变化情况

目的:探讨肝癌患者体液免疫和细胞免疫功能状况。方法:选取2012年1月20日至2015年3月25日在西宁市第一人民医院就诊的126例肝癌患者和87名健康者作为研究对象,采用免疫透射比浊法测定血清IgA、IgG水平,采用流式细胞术检测外周血T淋巴细胞亚群CD3~+、CD4~+、CD8~+和CD4~+/CD8~+水平。结果:与健康对照组相比,肝癌组患者血清中IgA和IgG水平显著升高(P0.05),外周血中T淋巴细胞亚群CD3~+、CD4~+和CD4~+/CD8~+显著降低(P0.05),CD8~+显著升高(P0.05)。结论:肝癌患者体液免疫功能增强,细胞免疫功能降低,外周血T细胞亚群和血清免疫球蛋白的检测对了解肝癌患者的病情、预后以及免疫治疗具有一定的临床指导意义。     

Reference ranges and age-related changes of peripheral blood lymphocyte subsets in Chinese healthy adults

This study was performed to build region-specific reference ranges of peripheral blood lymphocyte subsets for Chinese healthy adults from the young to the elderly and analyze the trends of changes in lymphocyte subsets for evaluating the impact of age on the values.151 healthy adults aged 19—86 were recruited based on the SENIEUR protocol.Three sets of reference ranges were finally built applicable for the healthy young(19—44 years),middle-aged(45—64 years) and elder adults(≥65).Comparisons in parameters am...     

Distribution and quantitative expression of the complement receptor type 1 (CR1) on human peripheral blood T lymphocytes.

The complement receptor, type 1 (CR1) is expressed on a variety of cell types including primate erythrocytes, phagocytic cells, and B lymphocytes. On these cells, CR1 plays a role in a diverse spectrum of biological activities including the clearance of immune complexes from the circulation, down-regulation of the complement system, recognition of complement-coated microorganisms, and cellular activation. CR1 is also expressed by some, but not all, T lymphocytes. The present study was undertaken in order to examine the distribution of CR1 on normal human T cell subsets by flow cytometry and to quantify the expression of T cell CR1 by radioimmunoassay. Data presented here indicate that, in a panel of 19 normal individuals, a mean of 9.7% of the overall peripheral blood lymphocyte population expressed CR1 and that, as assessed by two-color flow cytometry, 12.0% of CD3+, 13.0% of CD4+, and 20.0% of CD8+ cells expressed CR1. While single peaks of CR1 staining were observed within the CD3 and CD4 subsets, a biphasic pattern of staining was evident within the CD8 subset in which relatively high-intensity CR1 staining was detected within the subpopulation of "dull" CD8+ cells, whereas a lower intensity of CR1 staining was observed within the subpopulation of "bright" CD8+ cells. Duplicate analyses performed over a relatively short time frame suggested that, while the overall percentage of cells that expressed CR1 varied considerably among normal individuals, in at least some individuals the percentage of cells expressing CR1 was relatively stable, especially within the CD4 subset. In cell suspensions enriched for T lymphocytes by rosetting with sheep erythrocytes, 10.0% of the cells were CR1+ and a mean of approximately 3700 CR1 were expressed per CR1+ cell. There was no apparent correlation between the number of CR1 per T cell and the number of CR1 expressed per erythrocyte in the same blood sample. The expression of CR1 on subpopulations within the CD3+, CD4+, and CD8+ lymphocyte subsets may play a role in both normal cell function and in the pathophysiology of disease states including the acquired immune deficiency syndrome (AIDS).     

变应性鼻炎患者肠道微生态变化及其与T淋巴细胞亚群的关系

探讨变应性鼻炎患者肠道微生态变化及其与T淋巴细胞亚群的关系。

回顾性分析2019年4月至2020年4月医院收治的64例变应性鼻炎患者的资料, 记为A组; 另回顾性分析同期在该院体检的58例健康者的资料, 记为B组。对比A组和B组研究对象肠道菌群多样性和丰富度的变化, 对比A组和B组研究对象血清T淋巴细胞亚群水平, 分析变应性鼻炎患者肠道菌群相对丰度与血清T淋巴细胞亚群水平的相关性。

A组研究对象肠道菌群Chao1指数和Shannon指数均低于B组(P < 0.05), A组肠道菌群门水平拟杆菌门、变形菌门、厚壁菌门的相对丰度均高于B组(P < 0.05), 放线菌门的相对丰度低于B组(P < 0.05), 属水平毛螺菌属、链球菌属的相对丰度均高于B组(P < 0.05), 肠球菌属、双歧杆菌属、棒状杆菌属的相对丰度均低于B组(P < 0.05)。主成分分析显示2组菌群样品均能明显分开, 横纵坐标的贡献率分别为48.9%和16.7%, 两坐标轴总共解释了不同细菌群落差异的65.6%, 2组群落结构差异明显(P < 0.05)。A组CD3⁺比例、CD4⁺比例和CD4⁺/CD8⁺比值高于B组(P < 0.05), CD8⁺比例低于B组(P < 0.05);采用Pearson相关性检验, 变应性鼻炎患者肠道菌群门水平拟杆菌门、变形菌门和厚壁菌门的相对丰度分别与CD3⁺、CD4⁺比例和CD4⁺/CD8⁺比值呈正相关(P < 0.05), 分别与CD8⁺比例呈负相关(P < 0.05), 放线菌门的相对丰度分别与CD3⁺、CD4⁺比例和CD4⁺/CD8⁺比值呈负相关(P < 0.05), 与CD8⁺比例呈正相关(P < 0.05);属水平毛螺菌属和链球菌属的相对丰度分别与CD3⁺、CD4⁺比例和CD4⁺/CD8⁺比值呈正相关(P < 0.05), 分别与CD8⁺比例呈负相关(P < 0.05), 肠球菌属、双歧杆菌属和棒状杆菌属的相对丰度分别与CD3⁺、CD4⁺比例和CD4⁺/CD8⁺比值呈负相关(P < 0.05), 分别与CD8⁺比例呈正相关(P < 0.05)。

变应性鼻炎患者肠道菌群多样性降低, 门、属水平菌群比例均发生改变, 菌群结构差异明显, 血清CD3⁺、CD4⁺水平和CD4⁺/CD8⁺比值升高, CD8⁺水平降低, 变应性鼻炎患者肠道菌群相对丰度与血清T淋巴细胞亚群水平相关。

     

Phenotypical characterization of lymphocytes infiltrating regressing papillomas.

Papillomavirus-induced lesions often regress spontaneously in both humans and animals. Papilloma regression is deemed to be due to a cell-mediated immune response, the nature of which is still ill defined, and is accompanied by immune cell infiltrates. To gain further information on the nature and role of the immune cells present in regressing papillomas, we have analyzed biopsies of papillomas induced in the soft palate of cattle by bovine papillomavirus type 4 (BPV-4) and have phenotypically characterized and quantified the lymphocytes present in these lesions. Eleven papilloma biopsies and seven biopsies of noninfected palate were analyzed for the presence of activated CD4+, CD8+, and gamma delta(WC1+) lymphocytes. We found large numbers of lymphocytes in the subepithelial derma of papillomas but not in normal palate tissue; these cellular masses consisted predominantly of CD4+ lymphocytes, with only a few CD8+ and gamma delta(WC1+) lymphocytes, generally positioned at the periphery of these masses. All three subtypes of lymphocytes were found interdigitated with the cells of the basal layer both in papillomas and in normal palate tissue, but while basal layer CD8+ and gamma delta(WC1+) T cells were detected with similar frequencies in papillomas and uninfected palate, basal layer CD4+ T cells were much more frequent in papillomas. CD4+, CD8+, and gamma delta(WC1+) lymphocytes were found in the suprabasal layers of papillomas, but the CD8+ and gamma delta(WC1+) T cells were more numerous and had migrated further into the differentiating keratinocytes of the papilloma fronds than the CD4+ T cells. We conclude that T-cell infiltration is characteristic of regressing BPV-4 papillomas, that CD4+ lymphocytes are specifically and massively recruited into the regressing papillomas, and that although all three lymphocyte subsets can penetrate the papilloma, only the CD8+ and gamma delta(WC1+) lymphocytes are able to migrate into the fronds. These results suggest that all three lymphocyte subsets have an important role to fulfill during natural regression of papillomas.     

长春瑞滨联合卡铂化疗治疗晚期肺癌的临床观察

目的:探讨长春瑞滨联合卡铂化疗对晚期肺癌的治疗效果和安全性。方法:选取我院肿瘤科收治的晚期肺癌患者60例,根据不同治疗方案分为实验组与对照组,每组各30例患者。比较两组患者治疗前后的CEA、CA50、CYFRA21-1水平、白细胞计数及T淋巴细胞亚群CD4+、CD8+水平的变化。结果:治疗前,两组患者的CEA、CA50、CYFRA21-1水平、白细胞计数及T淋巴细胞亚群CD4+、CD8+水平比较均无统计学差异(P0.05)。治疗后,实验组的CEA、CA50、CYFRA21-1、CD8+水平均明显低于对照组,而白细胞计数及T淋巴细胞亚群CD4+、CD4+/CD8+水平均显著高于对照组,差异均具有统计学意义(P0.05)。结论:与放疗相比,长春瑞滨联合卡铂化疗能显著提高晚期肺癌的疗效,且不降低患者的细胞免疫。     

Effects of a short-term strength training programme on lymphocyte subsets at rest in elderly humans

The effects of a short-term strength training programme on resting lymphocyte subsets and stress hormone concentrations were analysed in 32 elderly sedentary subjects. Out of these 32 subjects, 8 women and 8 men [mean age 70.1 (SEM 1.0) years] were randomly assigned to a 8-week strength training programme which consisted of three sets of eight repetitions at 80% of one repetition maximum, for leg press, bilateral leg extension and seated chest press, 3 days a week. The remaining 8 women and 8 men [mean age 70.5 (SEM 0.9) years] served as controls. Absolute counts of lymphocyte subsets (CD20+, CD3+, CD3+CD4+, CD3+CD8+, CD3-CD56+CD16+) were measured with a new technique combining fluorescent microspheres and flow cytometry. In the trained subjects, substantial increases in strength took place in one repetition maximum during the 8-week training period for leg press [from means of 20.7 (SEM 1.0) to 23.6 (SEM 1.0) N x kg(-1) LBM (lean body mass)], chest press [from means of 5.4 (SEM 0.3) to 6.2 (SEM 0.3) N x kg(-1) LBM] and bilateral leg extension [from means of 6.3 (SEM 0.2) to 7.4 (SEM 0.3) N x kg(-1) LBM] movements. Baseline cortisol concentration (P < 0.01), CD20+ cell count (P < 0.05), CD3+ cell count (P < 0.05), and CD4+ cell count (P < 0.01) decreased in both groups secondary to circannual variations between winter and summer. No significant effect of strength training on resting adrenaline, noradrenaline and cortisol concentrations or distributions of lymphocyte subsets at rest was observed. The main finding of this study was to demonstrate that 8-week is too short a duration for a strength training programme to modify counts of lymphocyte subsets at rest in elderly sedentary adults.     

Generation of MHC-nonrestricted and restricted oncolytic subsets from human bone marrow.

We analyzed the cytotoxicity and characterized the phenotype of oncolytic bone marrow (BM) lymphocyte subsets generated in vitro by interleukin-2 (IL-2) and stimulator cells (SC). Two irradiated B-lymphoblastoid cell lines (Daudi and EBV-transformed BSM) and fresh human acute myelogenous leukemia (AML) were used as SC. Stimulation with Daudi and IL-2 resulted in a substantial increase in cytotoxic activity (100- to 1000-fold) against a broad range of tumor targets, and total cellular expansion was higher compared to stimulation with IL-2 alone. The most prominent increase was observed in the CD16+ and CD56+/CD3- natural killer (NK) cell subset; however, a significant increase was also observed in CD56+/CD3+ T cells. Functional analysis of Daudi- and IL-2-generated subsets using fluorescence-activated cell sorting (FACS) revealed that most of the lytic activity was mediated by NK cells. Significant potentiation of oncolytic activity and cell growth was also seen in the cultures stimulated with BSM or fresh AML and IL-2. The highest oncolytic activity in the latter cultures was mediated primarily by CD8+, CD3+, and CD56- T cells, although NK cells also participated in cytotoxic activity. The T cell-mediated cytotoxicity was restricted by the major histocompatibility complex (MHC), since most cytotoxicity could be blocked by HLA I antibodies. Additionally, we observed that optimum stimulation of cytotoxicity required effector cell-stimulator cell contact. These data indicate that depending on the tumor used for stimulation, different lymphocyte subsets may be generated in IL-2 cultures. These different approaches may be useful in both specific and nonspecific immunotherapy.     

Multi-way flow cytometric immune monitoring of antiretroviral therapy in HIV-infected children

In 40 HIV-infected children, 8-10 years old, belonging to the category A state of infection, the following flow-cytometric parameters were scored: percentage levels of different blood lymphocytes; surface expression of some activation and memory markers in CD4+ cells; switch to Th1 or Th2 of in vitro -stimulated CD4+ cell, tested by intracellular production of interleukin-2 or interleukin-4. Each investigation was carried out both before and 3 months after antiretroviral therapy (AZT and ddC). Some post-therapy changes concerning blood lymphocyte percentages were noticed, not only within CD4+ subpopulation, but also within CD8+, HLA-DR+/CD3 (T-activated) and CD16+CD56+ cells, respectively. On the other hand, following antiretroviral treatment, in HIV- originated CD4+ fresh cells, an improvement of pre-therapy increased values of surface activation (CD69, CD25) markers on memory (CD45RO+) cells, as well as of pre-therapy reduced rate of switching to Th1, revealed by intracellular interleukin-2 synthesis, was found. The significance of data obtained in the multi-way immune monitoring of antiretroviral therapy, in pediatric AIDS, as an additional investigation panel, is discussed.     

老年呼吸机相关性肺炎患者肠道菌群特征及其与疾病的相关性

探讨老年呼吸机相关性肺炎(VAP)患者肠道菌群特征及其与疾病的相关性, 为该类患者的治疗提供参考。

选择2018年1月至2020年1月在该院进行机械通气的患者141例, 根据是否发生VAP分为VAP组(n=67)和非VAP组(n=74), 同时选择同期在我院进行体检的健康者为对照组(n=50)。采用16S rRNA荧光定量PCR法检测3组研究对象肠道双歧杆菌和大肠埃希菌数量, 并计算B/E值。采用流式细胞仪检测T淋巴细胞亚群CD3⁺、CD3⁺CD4⁺、CD3⁺CD8⁺、CD4⁺/CD25⁺、CD8⁺/CD28^-以及CD8⁺/CD28⁺细胞水平。采用改良酶学分光光度法检测血浆D-乳酸水平。采用Pearson相关检验分析VAP组B/E值与免疫功能及血浆D-乳酸的相关性。

VAP组患者肠道双歧杆菌数量、B/E值显著低于非VAP组和对照组, 大肠埃希菌数量显著高于非VAP组和对照组(均P < 0.05);非VAP组和对照组比较差异亦均有统计学意义(均P < 0.05)。VAP组患者CD3⁺、CD3⁺CD4⁺、CD3⁺CD8⁺以及CD8⁺CD28⁺细胞水平显著高于非VAP组和对照组, CD8⁺CD28^-细胞水平显著低于非VAP组和对照组(均P < 0.05);非VAP组和对照组比较差异亦均有统计学意义(均P < 0.05)。VAP组患者血浆D-乳酸水平显著高于非VAP组和对照组, 非VAP组和对照组比较差异亦有统计学意义(均P < 0.05)。Pearson相关检验显示, VAP组患者B/E值与CD3⁺、CD3⁺CD4⁺、CD3⁺CD8⁺、CD8⁺/CD28⁺细胞以及血浆D-乳酸水平呈显著负相关, 与CD8⁺/CD28^-细胞呈显著正相关(均P < 0.05)。

VAP患者存在肠道菌群失衡, 且VAP与肠道菌群失衡存在相关性, 促进肠道菌群平衡有助于VAP的防治。

     

Phenotypically and functionally distinct subsets of natural killer cells in human PBMCs

Human natural killer (NK) cells are one major component of lymphocytes that mediate early protection against viruses and tumor cells, and play an important role in immune regulatory functions. In this study, we demonstrated that human NK cells could be divided into four subsets, CD56hi CD16(-), CD56lo CD16(-), CD56+CD16+ and CD56(-)CD16+, based on the expression of cell surface CD56 and CD16 molecules. Phenotypic analysis of NK cell subsets indicated that the expression of activation markers, adhesion molecules, memory cell markers, inhibitory and activating receptors, and intracellular proteins (granzyme B and perforin) were heterogeneous. Following interleukin (IL)-2 stimulation, interferon-gamma was preferentially produced by CD56+CD16(-) NK cells and this subset showed more proliferative capacity. The cytolytic activity of both CD56+CD16(-) and CD56+/-CD16+ subsets could be augmented in response to IL-2. The data provided a new definition for NK cell subsets demonstrating their phenotypic and functional diversity and possible stage of NK cell differentiation in peripheral blood.     

氟比洛芬酯对食管癌患者围术期外周血淋巴细胞亚群的影响

目的:探究氟比洛芬酯对食管癌患者围术期外周血淋巴细胞亚群的影响。方法:选择2014年6月~2016年8月期间在我院择期行食管癌根治术患者72例为研究对象,采用随机数字法将其分为氟比洛芬酯组(39例)和对照组(33例),患者均给予常规麻醉处理,对照组患者泵入5 mg托烷司琼与20μg/kg芬太尼;氟比洛芬酯组患者给予氟比洛芬酯2 mg/kg。评价术后12 h、24 h和48 h患者疼痛情况(VAS评分),并于术前1 h、术后24 h、术后72 h检测患者血清T细胞中CD3~+、CD4~+、CD8~+及CD56~+比例。结果:两组患者术后不同时刻VAS评分比较,差异均无统计学意义(P0.05);术后24 h两组患者CD3~+、CD4~+、CD4~+/CD8~+均显著降低(P0.05),术后72 h两组患者CD3~+、CD4~+、CD4~+/CD8~+水平均升高,且氟比洛芬酯组患者恢复到术后1 h水平,而对照组患者均仍低于术后1 h,且术后72 h氟比洛芬酯组CD3~+、CD4~+、CD4~+/CD8~+均高于对照组,差异均有统计学意义(P0.05),而CD8~+与CD56~+比例在两组各个时点均没有变化(P0.05);两组患者均未发生严重不良反应。结论:食管癌患者在手术过程中均出现免疫抑制,氟比洛芬酯麻醉效果较好,且对机体免疫功能具有保护作用,促进手术患者免疫功能的恢复,具有重要的临床价值。