共查询到20条相似文献,搜索用时 31 毫秒
1.
Estelle Thauvin Michelle Fusselier Josiane Arnaud Henri Faure Max Favier Charles Coudray Marie-Jeanne Richard Alain Favier 《Biological trace element research》1992,32(1-3):405-414
The effect of a multivitamin-mineral supplement was investigated during pregnancy according to a double-blind protocol by
determining zinc and copper in maternal plasma, mononuclear and polynuclear zinc and copper at the third, sixth, eighth, and
ninth months of gestation. The subjects were supplemented from the first trimester until delivery. A significant decrease
was observed in plasma zinc that varied from 11.5 μmol/L to 10.8 μmol/L in the supplemented group (n=29) and from 11 μmol/L to 10 μmol/L in the placebo group (n=33) at 3 and 9 mo of gestation, respectively. In contrast, plasma copper levels increased in a way depending upon the stage
of gestation in both groups: from 24.7 to 28.2 μmol/L in the treated group and from 24.9 to 30.9 μmol/L in the placebo group
at 3 and 9 mo of gestation, respectively, but the difference was only significant in the placebo group. No difference between
groups was observed in mononuclear and polynuclear zinc or copper levels.
These trace elements were also determined in cord blood at delivery. There were no statistically significant differences in
zinc and copper concentration found in placebo group and supplemented group. Finally, the beneficial effect of supplementation
on muscular cramps and appearance of vergetures was noted. 相似文献
2.
Hee Y. Paik Hyojee Joung Joo Y. Lee Hong K. Lee Janet C. King Carl L. Keen 《Biological trace element research》1999,69(1):45-57
The present study focused on whether serum extracellular superoxide dimutase (EC-SOD) activity can be used as a functional
indicator of marginal zinc deficiency in humans. Subjects in this study were 444 healthy adults over 30 yr of age living a
normal rural life in Kyunggi province, Korea. The mean dietary zinc intake of subjects obtained from one 24-h recall was 6.41
± 4.35 mg and the average serum zinc concentration of the subjects was 11.06 ± 2.44 (μmol/L. Subjects were divided into three
groups by serum zinc concentrations: adequate (serum zinc >10.7 (μmol/L), low (serum zinc 9.0–10.7 μmol/L), and very low (serum
zinc <9.0 μmol/L) groups. A total of 50 subjects were selected from the three groups for analysis of EC-SOD activities. The
EC-SOD activity of subjects increased with increasing serum zinc concentrations, and the activities of the three groups were
significantly different as indicated by the Kruskal-Wallis test (p = 0.0239). Also, serum EC-SOD activities were significantly
correlated with serum zinc concentrations (r = 0.289,p = 0.04). Serum EC-SOD activities, however, were not significantly correlated to the dietary zinc intakes. In conclusion,
these results show that EC-SOD activities are decreased in subjects with low serum zinc concentrations and suggest that EC-SOD
activity may be a functional indicator of zinc nutritional status in humans. 相似文献
3.
Jerome Honnorat Michele Accominotti Christiane Broussolle Andree-Carole Fleuret Jean-Jacques Vallon Jacques Orgiazzi 《Biological trace element research》1992,32(1-3):311-316
Zinc status was assessed in 53 diabetic patients: 18 insulin-dependent diabetic patients (IDDM), 22 noninsulin-dependent diabetic
patients (NIDDM) treated with oral antidiabetic agents, and 13 insulin-treated, noninsulin-dependent diabetic patients (IRDM).
Plasma zinc concentrations were in the usual range for healthy subjects in these three groups (15.3±0.9 μmol/L). Urinary zinc
excretions were elevated in the IDDM group (18.3±4.1 μmol/24 h;p<0.01 vs normal) and in the NIDDM group (17.5±3.5 μmol/24 h;p<0.01 vs normal), but normal in the IRDM group (11.3±2.4 μmol/24 h). In 14 NIDDM patients treated with transient continuous
sc insulin injections, urinary zinc decreased from 16.5±2.2 μmol/24 h before insulin treatment to 11.5±0.3 μmol/24 h after
insulin treatment without any modification in plasma zinc concentrations. 相似文献
4.
Marie J. Richard Veronique Ducros Michel Rorêt Josiane Arnaud Charles Coudray Michèle Fusselier Alain Favier 《Biological trace element research》1993,39(2-3):149-159
In six chronic dialyzed uremic patients, an intravenous sodium selenite (Se 50 μg during 5 wk and then 100 μg) and zinc gluconate
(Zn 5 mg) supplementation was performed during 20 wk at each dialysis session three times weekly. Before supplementation,
plasma Se and Zn, plasma and erythrocytes (RBC) antioxidant metalloenzymes glutathione peroxidase (GPX), and superoxide dismutase
(SOD) were significantly decreased, whereas lipid peroxidation (as thiobarbituric acid reactants TBARs) was increased. To
obtain a significative change in plasma selenium, we had to use an Se dose of 100 μg/dialysis session. Then, treatment-increased
plasma Se (from 0.58 ±0.09 to 0.89±0.16 μmol/L) led to a repletion of RBC-GPX (from 29.6±6 to 43±5.8 U/g Hb) and increased
plasma GPX levels (from 62±13 to 151±43 U/L). Plasma Zn and RBC-SOD did not vary significantly. The change of TBARs was not
observed between wk 1 and 4. They decreased significantly between wk 4 (4.80±0.21μmol/L) and wk 20 (4.16±0.26 μmol/L). We
noted a low correlation between TBARs and plasma GPX. A strong correlation was observed between Se and plasma GPX. The reversal
of Se deficiencies should reduce oxidative damage observed in these patients. 相似文献
5.
Henri Faure Alain Favier Michele Tripier Josiane Arnaud 《Biological trace element research》1990,24(1):25-37
In this paper we report a method for measuring ultrafiltrable zinc in human serum by electrothermal atomic absorption spectrophtometry.
We show also that ultrafiltration permits to determine alpha-2 macroglobulin bound zinc and losely bound zinc if a strong
zinc ligand (EDTA) is added to serum before ultrafiltration. This last fraction, after deduction of ultrafiltrable zinc, represents
roughly all albumin bound zinc. In 20 controls we found that ultrafiltrable zinc amounted 0.311 μmol/L (S.D.=0.117 μmol/L),
alpha-2 macroglobulin bound zinc 3.08 μmol/L (S.D.=0.221 μmol/L), and albumin bound zinc 12.11 μmol/L (S.D.=1.95 μmol/L).
Our method needs only a small volume of serum, it is simple and rapid but also very accurate and reliable. The losely bound
fraction is very dynamic and, representing the physiologically active part of serum zinc, it could be a good marker of zinc
deficiency. 相似文献
6.
Abdellatif Chakar Ridha Mokni Philip A. Walravens Philippe Chappuis Fanny Bleiberg-Daniel Jean-Louis Mahu Daniel Lemonnier 《Biological trace element research》1993,38(1):97-106
Plasma zinc, copper, and parameters of growth were measured in a group of 116 French preschool children, 2–5 yr-old from low-income
households. Participants were selected on the basis of Z-scores of weight for height (WHZ) and height for age (HAZ). Zinc
and copper concentrations of children with growth impairment (GI), defined by a WHZ and/or HAZ< −1 Z-score, were compared
to those of age, sex, and ethnic origin matched controls (WHZ and HAZ >−1 Z-score). Mean (±SD) plasma zinc concentration was
12.58±1.84 μmol/L in the GI group, and 13.27±1.98 μmol/L in the controls. The difference of the means of paired samples was
0.69±2.34, and by pairedt-test the significance reachedp=0.028. This effect was primarily a result of the weight retarded group (WHZ <−1 Z-score,p<0.009) and to the girls (p<0.05). There were no significant differences in plasma copper concentrations between groups. These results suggest the presence
of marginal zinc deficiency in French preschool children with low weight for height Z-scores. 相似文献
7.
Zinc absorption may be estimated by measuring the area under the plasma zinc curve following the ingestion of a zinc supplement.
The aim of this study is to determine the reproducibility of such a response when a small dose of zinc is administered to
healthy volunteers. Five female subjects were asked to consume 4.5 mg elemental zinc, and blood samples were obtained at 30
min intervals for 5 h. The experiment was repeated in the same volunteers 12–16 d later. The area under the plasma zinc curve
was 30% lower after the second zinc tolerance test compared with the first (11.0 vs 15.8 μmol/1 h). This difference could
not be explained by differences in the fasting plasma zinc levels (12.9 μmol/L Experiment one, 15.1 μmol/L Experiment 2) nor
was it related to technical or clinical parameters. The area under the curve after 5 h was strongly correlated with the response
after 4 h. Hence we conclude that a small dose of zinc can be used to determine zinc absorption and a shorter experimental
period may be used. However, trials must be designed to take into account the sequence variability in the response. 相似文献
8.
M. Bengoumi A. K. Essamadi J. C. Tressol B. Faye 《Biological trace element research》1998,63(2):81-94
At an experimental farm, five camels and five cows were fed a similar basal diet for 6 mo. They received oral trace element
supplementation for 3 mo (day 22–112). This supplementation included zinc, copper, selenium, managanese, iodine, and cobalt,
and corresponded to twice the requirements generally recommended for cows. Plasma copper and zinc concentrations were significantly
lower in the camels (61 μg/100 mL for copper and 38 μg/100 mL for zinc) than in the cows (111 and 83, respectively). The supplementation
had no effect on plasma zinc concentration in the camels in spite of the low observed values in this species. Liver copper
concentration at the beginning of the trial was lower in the camels (9 ppm) than in the cows (35 ppm), and stayed at lower
levels during the entire supplementation period. There was no clear difference in fecal excretion of copper and zinc between
the camels and the cows. The results suggested that trace element requirements are lower in camels than in cows and that camels
regulate their plasma zinc concentration at a very low level (<40 μg/100 mL). 相似文献
9.
Casimiro-Lopes G de Oliveira-Junior AV Portella ES Lisboa PC Donangelo CM de Moura EG Koury JC 《Biological trace element research》2009,127(2):109-115
The purpose of this study was to compare plasma leptin, plasma zinc, and plasma copper levels and their relationship in trained
female and male judo athletes (n = 10 women; n = 8 men). Blood samples were obtained 24 h after training to measure plasma zinc, copper, and leptin levels. Subjects presented
similar values to age (22 ± 2 years old), body mass index (24 ± 1 kg/m2), plasma zinc (17.2 ± 2 μmol/L), copper (12.5 ± 2 μmol/L), and leptin (5.6 ± 1.3 μg/L). However, height, total body mass,
lean mass, fat mass, and sum of ten-skinfold thickness were higher in male than female. Plasma leptin was associated with
sum of ten skinfolds in male (r = 0.91; p < 0.001) and female athletes (r = 0.84; p < 0.003). Plasma zinc was associated with leptin in males (r = 0.82; p < 0.05) while copper was associated with plasma leptin in females (r = 0.66; p < 0.05). Our results suggest that young judo athletes lost sex-related differences in leptin levels. Plasma zinc, plasma
copper, and energy homeostasis may be involved in regulation of plasma leptin. 相似文献
10.
Women with preeclampsia have been shown to have elevated blood levels of the metabolite homocysteine, and alterations in blood
levels of zinc and copper have also been reported. This study measured plasma levels of zinc, copper, and homocysteine in
women with preeclampsia and in women with healthy, normotensive pregnancies.
For the patients with preeclampsia compared with controls, significantly higher mean plasma levels were found of homocysteine
(16.39 vs 9.45 nmol/mL; p≤0.001), zinc (15.53 vs 11.93 μg/g protein; p < 0.05), and copper (47.90 vs 31.60 μg/g protein; p=0.001). The ratio of plasma Cu/Zn levels tended to be higher in preeclamptic women and could be taken as an index of inflammatory
reaction, but the difference was not significant. Homocysteine concentrations correlated positively with plasma zinc concentrations
in women with preeclampsia (r=0.588, p=0.003) but not in women with healthy pregnancies. No correlations were observed between plasma levels of homocysteine and
copper.
Thus, the present study found evidence that preeclampsia might be associated with hyperhomocysteinemia and elevated blood
levels of zinc and copper. Furthermore, elevated blood levels of zinc were significantly associated with hyperhomocysteinemia
in preeclampsia. More studies are warranted to investigate further any relationship between altered homocysteine metabolism
and levels of zinc and copper in preeclampsia. 相似文献
11.
Forrest H. Nielsen Terrence R. Shuler Thomas J. Zimmerman Michael E. Collings Eric O. Uthus 《Biological trace element research》1979,1(4):325-335
The interaction between nickel and iron was confirmed in rat metabolism. In a fully-crossed, two-way, three by four, factorially
designed experiment, female weanling rats were fed a basal diet supplemented with iron at 0, 25, 50, and 100 μg/g and with
nickel at 0, 5, and 50 μg/g. The basal diet contained about 10 ng of nickel and 2.3 μg of iron/g. After nine weeks, dietary
iron affected growth, hematocrit, hemoglobin, plasma cholesterol, and in liver affected total lipids, phospholipids, and the
contents of copper, iron, manganese, and zinc. By manipulating the iron content of the diet, effects of dietary nickel were
shown in rats that were not from dams fed a nickel-deprived diet. Nickel affected growth, hematocrit, hemoglobin, plasma alkaline
phosphatase activity, plasma total lipids, and in liver affected total lipids, and the contents of copper, manganese, and
nickel. The interaction between nickel and iron affected hematocrit, hemoglobin, plasma alkaline phosphatase activity, and
plasma phospholipids, and in liver affected size, content of copper, and perhaps of manganese and nickel. In severely iron-deficient
rats, the high level of dietary nickel partially alleviated the drastic depression of hematocrit and hemoglobin, and the elevation
of copper in liver. Simultaneously, high dietary nickel did not increase the iron level in liver and was detrimental to growth
and appearance of severely iron-deficient rats. In nickel-deprived rats fed the borderline iron-deficient diet (25 μg/g) hematocrit
and hemoglobin also were depressed. However, 5 μg Ni/g of diet were just as effective as 50 μg Ni/g of diet in preventing
those signs of nickel deprivation. The findings in the present study suggested that nickel and iron interact with each other
at more than one locus. 相似文献
12.
Laurent Noël Céline Huynh-Delerme Thierry Guérin Hélène Huet Jean-Marc Frémy Martine Kolf-Clauw 《Biometals》2006,19(5):473-481
The influence of long-term exposure to cadmium (Cd) on essential minerals was investigated using a Caco-2 TC7 cells and a
multi-analytical tool: microwave digestion and inductively coupled plasma mass spectrometry. Intracellular levels, effects
on cadmium accumulation, distribution, and reference concentration ranges of the following elements were determined: Na, Mg,
Ca, Cr, Fe, Mn, Co, Ni, Cu, Zn, Mo, and Cd. Results showed that Caco-2 TC7 cells incubated long-term with cadmium concentrations
ranging from 0 to 10 μmol Cd/l for 5 weeks exhibited a significant increase in cadmium accumulation. Furthermore, this accumulation was more marked
in cells exposed long-term to cadmium compared with controls, and that this exposure resulted in a significant accumulation
of copper and zinc but not of the other elements measured. Interactions of Cd with three elements: zinc, copper, and manganese
were particularly studied. Exposed to 30 μmol/l of the element, manganese showed the highest inhibition and copper the lowest on cadmium intracellular accumulation
but Zn, Cu, and Mn behave differently in terms of their mutual competition with Cd. Indeed, increasing cadmium in the culture
medium resulted in a gradual and significant increase in the accumulation of zinc. There was a significant decrease in manganese
from 5 μmol Cd/l exposure, and no variation was observed with copper. 相似文献
13.
The level and/or form of dietary iron, dietary nickel, and the interaction between them affected the trace element content
of rat liver. Livers were from the offspring of dams fed diets containing 10–16 ng, or 20 μg, of nickel/g. Dietary iron was
supplied as ferric chloride (30 μg/g) or ferric sulfate (30 μg, or 60 μg). In nickel-deprived rats fed 60 μg of iron/g of
diet as ferric sulfate, at age 35 days, levels of iron and zinc were depressed in liver and the level of copper was elevated.
At age 55 days, iron was still depressed, copper was still elevated, but zinc also was elevated. In rats fed 30 μg of iron/g
of diet as ferric chloride, liver iron content was higher in nickel-deprived than in nickel-supplemented rats at 30, but not
at 50, days of age. Also manganese and zinc were lower in nickel-deprived than in nickel-supplemented rats at age 35 days
if their dams had been on experiment for an extended period of time (i.e., since age 21 days). Thus, the levels of copper,
iron, manganese, and zinc in liver were affected by nickel deprivation, but the direction and extent of the affects depended
upon the iron status of the rat. 相似文献
14.
Zinc and copper have been estimated in CSF of 14 normal volunteers, nine men and five women. Zinc was analyzed by limited-aspiration
flame atomic absorption spectrophotometry using a deuterium continuum light source. Copper was analyzed in 0.1% HNO3 by flameless atomic absorption spectrophotometry with a graphite cuvette on a flameless atomizer. Recovery of added zinc
varied less than 5% and that of the added copper varied less than 8%. CSF zinc was 31.5±19.8 μg/L (mean ± 1 SD); CSF copper,
7.5±3.1 μ/L. Values obtained for CSF zinc are about 1/2 those we and others obtained previously, the decrease related almost
exclusively to removal of interference by the CSF matrix, which produced spuriously elevated values without use of the deuterium
light source. Values obtained for CSF copper were approximately one-tenth those we and others had obtained previously. The
decrease related, in part, to the removal of matrix effects, but also to improvement of the signal-to-noise ratio present
in other techniques. 相似文献
15.
The effects of selenium, zinc, iron, chromium, and lead on telomere lengths of human cells have not been investigated. This
article adopted flow cytometry and fluorescence in situ hybridization to investigate the impact of different elements on cellular apoptosis and telomere lengths of human hepatocytes
L-02 and hepatoma cells SMMC-7721. Results showed that these trace elements under the following dosages did not have remarkable
effect on cellular apoptosis. However, sodium selenite at doses of 0.5 and 2.5 μmol/L significantly extended the telomere
length of hepatocytes L-02; 0.5 μmol/L lead acetate remarkably shortened the telomere length of L-02 cells; 80 μmol/L zinc
sulfate, 20 μmol/L ferric chloride, and 200 μmol/L chromic chloride only had slight impact on the telomere length, respectively.
Regarding hepatoma cells SMMC-7721, sodium seleite at 0.5 and 2.5 μmol/L had little impact on the telomere length; 80 μmol/L
zinc sulfate significantly accelerated the loss of telomere length, whereas 20 μmol/L ferric chloride, 200 μmol/L chromic
chloride, and 0.5 μmol/L lead acetate remarkably extended the telomere lengths, respectively. The results revealed differential
effects of each trace element on the life-span of human hepatocytes and hepatoma cell lines, which suggested further research
on somatic hepatocytes and hepatoma in vivo. 相似文献
16.
Regional distribution of metallothionein, zinc, and copper in the brain of different strains of rats 总被引:4,自引:0,他引:4
The regional brain distribution of metallothionein (MT), zinc, and copper in the brain was determined in nine anatomical regions
(olfactory bulb, cortex, corpus striatum, hippocampus, thalamus plus hypothalamus, pons plus medulla oblongata, cerebellum,
midbrain, and white matter) and was compared between two different strains of rat (Sprague-Dawley [SD] and Lewis). No significant
difference was observed in the whole-brain MT level between the two strains (17.8 ± 3.4 μg/g in SD rats and 20.3 ± 2.3 μg/g
in Lewis rats). In SD rats, however, MT was more highly expressed in the white matter than in the other regions studied. In
contrast, MT concentration was highest in the cortex and lowest in the olfactory bulb in Lewis rats. The MT levels in the
cortex, corpus striatum, hippocampus, and thalamus plus hypothalamus were significantly lower in SD rats than in Lewis rats.
In both strains, the olfactory bulb contained markedly higher levels of both zinc and copper than the other regions (27.9
±6.8 μg/g zinc in SD rats and 27.6 ± 6.9 μg/g zinc in Lewis rats, and 5.2 ± 1.5 μg/g copper in SD rats and 11.1 ± 4.8 μg/g
copper in Lewis rats). The next high-est zinc levels were seen in the hippocampus, whereas the next highest copper levels
were in the corpus striatum in both SD and Lewis rats. The high levels of zinc and copper in the olfactory bulb were not accompanied
by concomitant high MT concentrations. These results indicate that the strain of rat as well as the anatomical brain region
should be taken into account in MT and metal distribution studies. However, the highest concentrations of zinc and copper
in olfactory bulb were common to both SD and Lewis rats. The discrepancy between MT and the metal levels in olfactory bulb
suggests a role for other proteins in addition to MT in the homeostatic control of zinc and copper. 相似文献
17.
The concentrations of cadmium, lead, selenium, and zinc in blood and seminal plasma were determined in 76 Singapore males.
Except for zinc, the concentrations were generally higher in blood than in seminal plasma (cadmium, 1.31 μg/L vs 0.61 μg/L;
lead, 82.6 μg/L vs 12.4 μg/L, and selenium, 163.6 μg/L vs 71.5 μg/L). The mean concentration of zinc in seminal plasma was
more than 30 times higher than in blood (202 mg/L vs 6.2 mg/L). Significant positive correlations were found between the concentrations
in blood and seminal plasma for the two essential trace elements: selenium (r=0.45,p<0.001) and zinc (r=0.25,p<0.05). However, no relationships were found between the concentrations in blood and seminal plasma for two toxic metals (cadmium
and lead). Significant inverse correlations were observed between Cd and Zn (r=−0.40,p<0.01), and Pb and Se (r=−0.32,p<0.05) in blood, whereas significant positive correlations were noted between Cd and Se (r=0.45,p<0.01), Cd and Zn (r=0.35,p<0.05), and Se and Zn (r=0.57,p<0.001) in seminal plasma. The physiological significance of these relationships are also discussed in this paper. 相似文献
18.
Matti Tolonen Seppo Sarna Markku Halme Seppo E. J. Tuominen Tuomas Westermarck Ulla-Riitta Nordberg Mirja Keinonen Jaap Schrijver 《Biological trace element research》1988,17(1):221-228
An “anti-oxidant cocktail” consisting of betacarotene, vitamins B6, C, E, zinc, and selenium or corresponding placebos were given for one y as daily dietary supplements to 45 elderly residents
of a nursing home. Initially, the serum TBA reactant levels were higher (2.7±0.7 μmol/L) than those of an ad hoc control group
of healthy younger adults (2.3±0.6 μmol/L),p<0.01. After three mo supplementation, the levels among the verum elderly had decreased to 2.2±0.6 μmol/L, and they remained
at this lower level until the end of the study period, whereas the placebo group showed no change.
A significant inverse correlation (r=−0.428,p<0.01) existed between the concentrations of serum TBA reactants and whole blood selenium (B-Se), but only B-Se levels above
200 μg/L were associated with a decrease in serum lipid peroxides. Serum alpha-tocopherol concentration also correlated inversely
with serum TBA reactants but this correlation (r=−0.273,p<0.76) was not as strong as that of B-Se.
Deficient vitamin B6 status, in biochemical terms, was observed in 25% of the elderly; a daily supplement of 2 mg B6 fully cured all cases of deficiency. The verum group improved slightly in several psychological tests, whereas subjects on
placebo remained unchanged or deteriorated during the follow-up period. Clinical amelioration among the verum subjects was
reported by the nurses; no toxic side effects were reported. In conclusion, the elderly benefited biochemically and clinically
of dietary antioxidant supplements. 相似文献
19.
Rapid and direct determination of selenium, copper, and zinc in blood plasma by flow injection-inductively coupled plasma-mass spectrometry 总被引:1,自引:0,他引:1
A flow injection-inductively coupled plasma-mass spectrometry (FI-ICP-MS) with a simple sample preparation procedure was developed
for the determination of selenium, copper, and zinc in blood serum/plasma. A serum/plasma sample was filtered through a 0.45-μm
membrane filter and diluted with a mixture of trace elements in a standard solution (9∶1, v/v). Measurement of the reference
serum sample confirmed the accuracy of our method for selenium, copper, and zinc concentration. In the case of blood plasma
samples obtained from six healthy adult males, the selenium, copper, and zinc concentrations were similar to those of a typical
healthy male in Japan. These results suggest that the sample prepartive procedure coupled with FI-ICP-MS can be used for the
routine determination of selenium, copper, and zinc in human blood serum/plasma. 相似文献
20.
Copper and zinc act as a cofactor of over 300 mammalian proteins. Both have same electronic configuration therefore they are
antagonist at higher individual concentration. The present study was designed with the aim to investigate the mechanisms pertaining
to toxic effects of copper on human peripheral blood mononuclear cells (PBMCs) and to evaluate the cytoprotective effect of
zinc on copper-induced cytotoxicity. The copper uptake into PBMCs was progressively increased with increasing concentration
of metal in the growth medium. However, no significant effect on copper uptake was observed in the presence of zinc. Cell
proliferation rate was decreased with increasing copper concentration. Interestingly, the proliferation rate of zinc treated
PBMCs remained nearly the same as that of control cells. LD50 of copper (115 μM) was increased six times (710 μM) in presence of zinc for PBMCs. At higher concentrations of copper (>
100 μM) decrease level of GSH was noticed. Increased levels of metallothionein in PBMCs were observed in response to zinc.
DNA fragmentation studies also showed that copper produced DNA fragmentation at LD50 (115 μM). Subsequently, zinc showed protection against DNA fragmentation caused by copper. Cell structure of PBMCs at LD50 (115 μM copper) showed membrane bound cystic spaces and mitochondria having disrupted cristae and few myelin figures. In
presence of zinc at LD50 of copper (115 μM) cells showed improvement in mitochondrial structure and membrane bound cystic spaces. Taken together,
the results of our study demonstrates that zinc play an important role in prevention of copper toxicity in peripheral blood
mononuclear cells. 相似文献