共查询到20条相似文献,搜索用时 46 毫秒
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Change of function of the wheat stress‐responsive transcriptional repressor TaRAP2.1L by repressor motif modification
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Amritha Amalraj Sukanya Luang Manoj Yadav Kumar Pradeep Sornaraj Omid Eini Nataliya Kovalchuk Natalia Bazanova Yuan Li Nannan Yang Serik Eliby Peter Langridge Maria Hrmova Sergiy Lopato 《Plant biotechnology journal》2016,14(2):820-832
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Soybean DREB1/CBF‐type transcription factors function in heat and drought as well as cold stress‐responsive gene expression
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Satoshi Kidokoro Keitaro Watanabe Teppei Ohori Takashi Moriwaki Kyonoshin Maruyama Junya Mizoi Nang Myint Phyu Sin Htwe Yasunari Fujita Sachiko Sekita Kazuo Shinozaki Kazuko Yamaguchi‐Shinozaki 《The Plant journal : for cell and molecular biology》2015,81(3):505-518
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Xenopus egg extracts initiate replication at specific origin sites within mammalian G1‐phase nuclei. Similarly, S‐phase extracts from Saccharomyces cerevisiae initiate DNA replication within yeast nuclei at specific yeast origin sequences. Here we show that Xenopus egg extracts can initiate DNA replication within G1‐phase yeast nuclei but do not recognize yeast origin sequences. When G1‐phase yeast nuclei were introduced into Xenopus egg extract, semiconservative, aphidicolin‐sensitive DNA synthesis was induced after a brief lag period and was restricted to a single round of replication. The specificity of initiation within the yeast 2 μm plasmid as well as in the vicinity of the chromosomal origin ARS1 was evaluated by neutral two‐dimensional gel electrophoresis of replication intermediates. At both locations, replication was found to initiate outside of the ARS element. Manipulation of both cis‐ and trans‐acting elements in the yeast genome before introduction of nuclei into Xenopus egg extract may provide a system with which to elucidate the requirements for vertebrate origin recognition. J. Cell. Biochem. 80:73–84, 2000. © 2000 Wiley‐Liss, Inc. 相似文献
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酵母单杂交的原理与应用实例 总被引:3,自引:0,他引:3
许多诱导型基因的表达,都受特定的转录因子和顺式元件调控。要阐明各种信号传递途径与基因表达调控的机理,克隆和鉴定转录因子是关键。近年来酵母单杂交方法被广泛应用于克隆和鉴定各种动植物的转录因子,本文以拟南芥DREB转录因子的克隆为例,介绍酵母单杂交方法的原理和具体应用 。 相似文献
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H. Boetti L. Chevalier L. A. Denmat D. Thomas B. Thomasset 《Biotechnology and bioengineering》1999,64(1):1-13
In this study, the efficiency of inducible promoters to switch on gene expression in the presence of inducer or to switch it off in its absence was evaluated in tobacco cell suspensions transformed with the gus gene coding sequence. Either plant (pats1A, pSalT, pIn2‐2) or microbial (pMre, pTet) inducible promoters were used to drive gus expression. The inducers were light, abscisic acid, 2‐CBSU, CuSO4, tetracycline, respectively. For each construct (inducible promoter‐gus coding sequence), the optimal induction conditions were determined (inducer concentration, induction time, and age of cells in culture cycle before induction). The efficiency of the inducible promoter was then evaluated under optimal induction conditions. GUS‐expression levels obtained under non‐inducing and inducing conditons were systematically compared. Thirty or forty percent of the clones transformed with the pSalT‐gus or pTet‐gus construct, respectively, showed high induction rates (>1000) and GUS activities of the same order as those obtained with a constitutive system. However, basal GUS levels were always high for the pTet‐gus cell lines. Seventy or eighty‐five percent of the cell lines transformed with the pMre‐gus or pln2‐2‐gus construct, respectively, had induction rates of 1.5 to 1000. The pats1A‐gus construct gave very low induction rates—55% of cell lines had induction rates less than 1.5. Only the pSalt‐gus construct gave both the highest induction rates and basal GUS‐levels equivalent to the endogenous GUS background. © 1999 John Wiley & Sons, Inc. Biotechnol Bioeng 64: 1–13, 1999. 相似文献
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