共查询到20条相似文献，搜索用时 0 毫秒
1.
Katsunori Teranishi 《Luminescence》2016,31(2):594-599
Mycena chlorophos, which is primarily distributed in Southeast Asia, is a luminous fungus that emits a bright green light from its pileus for about 2 days at approximately 20°C and high relative humidity. The distribution of bioluminescent tissues in the whole pileus and its sections was heterogeneous. The light intensity in the cap and the upper region of the gill was greater than that in the lower region of the gill. At the microscopic level, the light was predominantly emitted from the membranes of hymenium and basidia cells on the gill. The emission was both cell and region specific. The luminescence system was localized in the cell membrane, and a part of the system was on the cell membrane surface. Copyright © 2015 John Wiley & Sons, Ltd. 相似文献
2.
Katsunori Teranishi 《Luminescence》2017,32(5):866-872
The chemical mechanisms underlying visible bioluminescence in the fungus Mycena chlorophos are not clear. A combination of dihydronicotinamide adenine dinucleotide phosphate (NADPH) and hispidin, which has been reported to increase the intensity of in vitro luminescence in crude cold‐water extracts prepared from the bioluminescent fruiting bodies of M. chlorophos, exhibited potential bioluminescence activation in the early bioluminescence stages, in which the bioluminescence was ultra‐weak, for living gills and luminescence activation for non‐bioluminescent gills, which was collapsed by freezing and subsequent thawing, at all bioluminescence stages. These abilities were not evident in considerably bioluminescent gills. These abilities were blocked by trans‐4‐hydroxycinnamic acid and trans‐3,4‐dihydroxycinnamic acid, which were identified as in vivo bioluminescence‐activating components. Original bioluminescence and bioluminescence produced from the addition of trans‐4‐hydroxycinnamic acid and trans‐3,4‐dihydroxycinnamic acid in living gills were almost completely inhibited by 10 mM NaN3, whereas the luminescence produced form the combination of NADPH and hispidin in thawed non‐bioluminescent and living gills at the early weak bioluminescence stages was not inhibited by 10 mM NaN3. Thus, the combination of NADPH and hispidin plays different roles in luminescence systems compared with essential bioluminescence systems, and the combination of NADPH and hispidin was not essential for visible bioluminescence in living gills. 相似文献
3.
Katsunori Teranishi 《Luminescence》2017,32(2):182-189
Mycena chlorophos is an oxygen‐dependent bioluminescent fungus. The mechanisms underlying its light emission are unknown. A component that increased the bioluminescence intensity of the immature living gills of M. chlorophos was isolated from mature M. chlorophos gills and chemically characterized. The bioluminescence‐activating component was found to be trans‐3,4‐dihydroxycinnamic acid and its bioluminescence activation was highly structure‐specific. 13C‐ and 18O‐labelling studies using the immature living gills showed that trans‐3,4‐dihydroxycinnamic acid was synthesized from trans‐4‐hydroxycinnamic acid in the gills by hydroxylation with molecular oxygen as well as by the general metabolism, and trans‐3,4‐dihydroxycinnamic acid did not produce hispidin (detection‐limit concentration: 10 pmol/1 g wet gill). Addition of 0.01 mM hispidin to the immature living gills generated no bioluminescence activation. These results suggested that the prompt bioluminescence activation resulting from addition of trans‐3,4‐dihydroxycinnamic acid could not be attributed to the generation of hispidin. Copyright © 2016 John Wiley & Sons, Ltd. 相似文献
4.
Cultural conditions on mycelial growth and fruit-body formation ofMycena chlorophos were studied. The optimum temperature of the mycelial growth was 27°C and the optimum initial pH of medium was 4.0. Peptone agar medium was suitable for the spawn culture. Compost medium containing rice bran at 10% (fw/fw) was appropriate for fruitbody formation in the Petri dish. Light was essential for initiation of primordia, and low-temperature treatment induced fruit-body formation effectively. The optimum conditions for fruit-body formation were found to be the cultivation at 27°C for 4 wk and continued cultivation for 3 wk under illumination at an intensity above 0.2 lx and at 21°C after casing with moist compost powder. In the fruit-bodies obtained, the maximum photosensitive wavelength of luminescence was 522 nm and the optimum temperature for emission was 27°C. The luminescence of a fruit-body was observed for about 3 d consecutively at 21°C. 相似文献
5.
Cultural conditions for fruit-body production ofMycena chlorophos were investigated with the aim of using the mushroom for study of bioluminescence, scientific exhibition, and ecological conservation. A small glass jar having a cap with a microfilter was used as a culture vessel. A compost powder mixed with rice bran in proportion of 20% (fw/fw) and adjusted to 70% (w/w) in moisture content was used as production medium. Casing with 2 g/jar of moistened compost powder was necessary for fruit-body formation. Mycelium was grown in a culture chamber at 27°C, relative humidity (RH) of 80% for 4 wk, then transfered to a culture chamberat 21°C, 90% RH and light intensity of 300–800 lx after casing its, and incubated for 3 wk to produce fruit-bodies. The mean yield was 31 fruitbodies, i.e., 150 mg dry weight per jar. 相似文献
6.
The chemical structure of two luciferin precursors PS-A and PS-B, isolated from the luminous mushroom Panellus stipticus, were determined as 1-O-decanoylpanal (2) and 1-O-dodecanoylpanal (3), respectively. Both PS-A and PS-B were converted into chemiluminescent luciferins by treatment with 50 mmol/l methylamine in a pH 3.5 buffer solution containing an anionic surfactant Tergitol 4 at 25–35ºC. The luciferins emitted chemiluminescence in a pH 7–8 buffer solution containing a cationic surfactant in the presence of O2 and O. 相似文献
7.
本文报道了广东省小菇属 Mycena(Pers.ex Fr.)s.F.Gray 的21个种,其中新种3个,国内新记录种4个以及新组合1个。新种是鼎湖小菇 Myce-na dinghuensis Bi sp.nov.,近细小菇 Mycena subgracilis Bi sp.nov.以及亚长刺小菇 Mycena sublongiseta Bi sp.nov.。新组合为拟安络小菇 Mycena pseudo-androsacea(Bull.ex Fr.)Bi comb.nov;新记录种是:唯一小菇 Mycena insignis Smith,狭褶小菇 Mycena leptophylla(Pk.)Sacc.,粗糙孢小菇 Mycena trachyspora Rea 以及特洛伊小菇 Mycena,tro-jana Murr.。 相似文献
8.
9.
10.
长白山地区小菇属Mycena类群种类繁多,研究发现采自长白山地区的3个中国小菇属新记录种,即弯柄小菇M.mirata、胶盖小菇M.renati和淡红小菇M.roseocandida,三者的盖皮层菌丝均有不同程度的疣状突起。通过形态学研究,详尽描述了三者的宏观形态特征、显微结构以及与相近种类的区别,并提供线条图。研究标本存放在吉林农业大学菌物标本馆(HMJAU)。 相似文献
11.
Aims: To increase the fruit body production of Cordyceps guangdongensis, selected cultivation conditions, especially nutritional parameters were optimized. Methods and Results: Cordyceps guangdongensis was inoculated on potato dextrose agar slants with pH values from 4·5 to 9·0 and cultivated in artificial media with different carbon and nitrogen supplements. Primordium formation in C. guangdongensis was favoured by slightly acidic conditions. Fruit body yields and biological efficiency (BE) recorded were all highest in cultures of C. guangdongensis supplemented with sucrose and KNO3 as carbon and nitrogen supplements, respectively. Highest fruit body yields and BE values were recorded with C : N ratio of 12 : 1. The optimal medium consisted of (g l?1) 20·0 sucrose, 4·0 soya bean powder, 5·0 beef extract and 10·0 KNO3. Cultivation experiments using this medium confirmed its reliability; 18·35% of BE was obtained, compared with a calculated maximum BE of 18·65% based on orthogonal test data. Conclusions: Cordyceps guangdongensis preferred sucrose and potassium nitrate as best carbon and nitrogen supplements. It produced satisfying yield of fruit body with optimized medium. Significance and Impact of the Study: Optimized artificial cultivation conditions could promote the yield of C. guangdongensis and decreased the cost of production. 相似文献
12.
Pei-Sheng Yan Xin-Chang Luo Qi Zhou 《World journal of microbiology & biotechnology》2004,20(8):795-799
Auricularia mushrooms are the fourth most important cultivated mushrooms in the world, with a unique jelly taste and horizontal-septated basidium which are significantly different from other cultivated mushrooms. Differentiation of commercial cultivated strains is difficult to conduct, due to the lack of useful distinguishable characters. In this study, we used the RAPD technique to differentiate 11 commercial strains of A. auricula and five commercial strains of A. polytricha and one white-fruitbody mutant strain, and to characterize their genetic diversity. Results showed that all the strains tested could be differentiated by pooled RAPD data, and even one individual primer (S10) could also discriminate all tested strains. RAPD analysis could differentiate strains having identical rDNA RFLP and supports the classification of the white-fruitbody mutant to the species of A. polytricha. Genetic similarity analysis and grouping derived from RAPD markers reveals a high level of genetic diversity of commercial strains of Auricularia auricula and A. polytricha. Therefore, the RAPD technique can provide a powerful tool to discriminate the commercialAuricularia strains and offer the molecular information useful for breeding systems. 相似文献
13.
墨兰共生真菌一新种的分离培养鉴定及其生物活性 《菌物学报》1996,15(4)
在对产于云南西双版纳的热带兰花菌根进行研究时,发现一分离自墨兰的菌株,菌丝纯白色,具典型的锁状连合,对其子实体的鉴定表明该菌株代表着一新分类单位——兰小菇Mycena orchidicola Fan et Guo sp.nov.同时,对该新种在兰科种子萌发中的作用作了初步探讨,结果表明该菌可侵入兰科种子,促进种子萌发。 相似文献
14.
Peñas MM Azparren G Domínguez A Sommer H Ramírez L Pisabarro AG 《Molecular genetics and genomics : MGG》2005,274(4):402-409
15.
《Mycoscience》2020,61(5):259-263
Imidazole-4-carboxamide (ICA) and 2-azahypoxanthine (AHX) obtained from Lepista sordida inhibit and promote the growth of herbaceous plants, respectively. In this study, we examined the effects of these compounds on the growth and ectomycorrhizal (EM) colonization of Pinus densiflora seedlings inoculated with Tricholoma matsutake that forms EM associations with pines. The EM colonization by T. matsutake was observed on the root systems of P. densiflora seedlings treated with and without ICA and AHX. The growth of both non-EM and EM P. densiflora seedlings was inhibited by ICA, regardless of the EM colonization. In contrast, AHX promoted the growth of non-EM P. densiflora seedlings, but not of EM seedlings, suggesting that EM colonization interferes with the effect of AHX on P. densiflora growth. 相似文献
16.
17.
报道了6个吉林省新记录担子菌,即辅毛鬼伞[Coprinus radians(Desm.:Fr.)Fr.]、金黄拟蜡伞[Hy-grophoropsis aurantiaca(Wulf.:Fr.)Maire]、黄柄小菇[Mycena epipterygia(Scop.:Fr.)S.F.Gray]、黏柄小菇[Mycenarorida(Scop.:Fr.)Qu啨l.]、长齿白齿耳菌[Mycoleptodonoides aitchisonii(Berk.)Mass.]和大刷革[Xylobo-lus princeps(Jungh.)Boidin]。其中,黏柄小菇同时为中国新记录种,其他5种首次在我国东北地区发现,拟蜡伞属和白齿耳菌属为吉林省新记录属。标本保存于吉林农业大学菌物标本馆(HMJAU)。 相似文献
18.
Mycena haushoferi, a new species of the section Intermediae collected in Bavaria, is described and compared with four other species of the sect. Intermediae known from the Northern Hemisphere and with M. cystidiosa and M. metuloidifera, two species of sect. Metuloidiferae. The five known species of Northern Hemisphere of section Intermediae are keyed out. 相似文献
19.
Kevin R. Cloonan Stefanos S. Andreadis Thomas C. Baker 《Entomologia Experimentalis et Applicata》2016,159(3):298-304
To evaluate the attractiveness of several mushroom‐growing substrates to the female mushroom fly Lycoriella ingenua (Dufour) (Diptera: Sciaridae), a pest of the cultivated white button mushroom, Agaricus bisporus (JE Lange) Emil J Imbach (Agaricales), we developed a two‐choice, static‐flow olfactometer. Behavioral assays using this olfactometer indicated that mushroom compost with A. bisporus mycelia growing in it was not more attractive than compost lacking growing mycelia. We also found that female flies were more attracted to compost lacking A. bisporus mycelia than to the actual commodity, the white button mushroom fruiting bodies. Flies were not, however, attracted to sterilized compost, suggesting the attraction is due to volatiles produced by microbial metabolism in the compost. We also found that female L. ingenua flies were attracted to the mycoparasitic green mold Trichoderma aggressivum Samuels & W Gams (Hypocreales). Flies preferred mushroom compost that had T. aggressivum growing in it over compost lacking T. aggressivum, providing an experimental outcome consistent with the anecdotal belief that L. ingenua flies are vectors of T. aggressivum spores that can infest mushroom‐growing houses. 相似文献