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1.
Development and mapping of SSR markers for maize 总被引:45,自引:0,他引:45
Sharopova N McMullen MD Schultz L Schroeder S Sanchez-Villeda H Gardiner J Bergstrom D Houchins K Melia-Hancock S Musket T Duru N Polacco M Edwards K Ruff T Register JC Brouwer C Thompson R Velasco R Chin E Lee M Woodman-Clikeman W Long MJ Liscum E Cone K Davis G Coe EH 《Plant molecular biology》2002,48(5-6):463-481
Microsatellite or simple sequence repeat (SSR) markers have wide applicability for genetic analysis in crop plant improvement strategies. The objectives of this project were to isolate, characterize, and map a comprehensive set of SSR markers for maize (Zea mays L.). We developed 1051 novel SSR markers for maize from microsatellite-enriched libraries and by identification of microsatellite-containing sequences in public and private databases. Three mapping populations were used to derive map positions for 978 of these markers. The main mapping population was the intermated B73 × Mo17 (IBM) population. In mapping this intermated recombinant inbred line population, we have contributed to development of a new high-resolution map resource for maize. The primer sequences, original sequence sources, data on polymorphisms across 11 inbred lines, and map positions have been integrated with information on other public SSR markers and released through MaizeDB at URL:www.agron.missouri.edu. The maize research community now has the most detailed and comprehensive SSR marker set of any plant species. 相似文献
2.
Insertion-deletion polymorphisms in 3′ regions of maize genes occur frequently and can be used as highly informative genetic markers 总被引:6,自引:0,他引:6
Bhattramakki D Dolan M Hanafey M Wineland R Vaske D Register JC Tingey SV Rafalski A 《Plant molecular biology》2002,48(5-6):539-547
Single-nucleotide polymorphisms (SNPs) are the most frequent variations in the genome of any organism. SNP discovery approaches such as resequencing or data mining enable the identification of insertion deletion (indel) polymorphisms. These indels can be treated as biallelic markers and can be utilized for genetic mapping and diagnostics. In this study 655 indels have been identified by resequencing 502 maize (Zea mays) loci across 8 maize inbreds (selected for their high allelic variation). Of these 502 loci, 433 were polymorphic, with indels identified in 215 loci. Of the 655 indels identified, single-nucleotide indels accounted for more than half (54.8%) followed by two- and three-nucleotide indels. A high frequency of 6-base (3.4%) and 8-base (2.3%) indels were also observed. When analysis is restricted to the B73 and Mo17 genotypes, 53% of the loci analyzed contained indels, with 42% having an amplicon size difference. Three novel miniature inverted-repeat transposable element (MITE)-like sequences were identified as insertions near genes. The utility of indels as genetic markers was demonstrated by using indel polymorphisms to map 22 loci in a B73 × Mo17 recombinant inbred population. This paper clearly demonstrates that the resequencing of 3 EST sequence and the discovery and mapping of indel markers will position corresponding expressed genes on the genetic map. 相似文献
3.
Maize adaptation to temperate climate: relationship between population structure and polymorphism in the Dwarf8 gene
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Camus-Kulandaivelu L Veyrieras JB Madur D Combes V Fourmann M Barraud S Dubreuil P Gouesnard B Manicacci D Charcosset A 《Genetics》2006,172(4):2449-2463
To investigate the genetic basis of maize adaptation to temperate climate, collections of 375 inbred lines and 275 landraces, representative of American and European diversity, were evaluated for flowering time under short- and long-day conditions. The inbred line collection was genotyped for 55 genomewide simple sequence repeat (SSR) markers. Comparison of inbred line population structure with that of landraces, as determined with 24 SSR loci, underlined strong effects of both historical and modern selection on population structure and a clear relationship with geographical origins. The late tropical groups and the early "Northern Flint" group from the northern United States and northern Europe exhibited different flowering times. Both collections were genotyped for a 6-bp insertion/deletion in the Dwarf8 (D8idp) gene, previously reported to be potentially involved in flowering time variation in a 102 American inbred panel. Among-group D8idp differentiation was much higher than that for any SSR marker, suggesting diversifying selection. Correcting for population structure, D8idp was associated with flowering time under long-day conditions, the deletion allele showing an average earlier flowering of 29 degree days for inbreds and 145 degree days for landraces. Additionally, the deletion allele occurred at a high frequency (>80%) in Northern Flint while being almost absent (<5%) in tropical materials. Altogether, these results indicate that Dwarf8 could be involved in maize climatic adaptation through diversifying selection for flowering time. 相似文献
4.
Genetic analysis of drought tolerance in maize by molecular markers. II. Plant height and flowering 总被引:1,自引:0,他引:1
M. Sari-Gorla P. Krajewski N. Di Fonzo M. Villa C. Frova 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1999,99(1-2):289-295
Drought is a serious agronomic problem, and one of the most important factors contributing to crop yield loss. In maize grown in temperate areas, drought stress occurs just before and during the flowering period; consequently, tolerance to water stress in this species is largely determined by events that occur at or shortly after flowering. The purposes of our investigation were: (1)?to identify the chromosomal regions where factors conferring drought tolerance for traits related to plant development and flowering are located and (2)?to compare these regions with those carrying QTLs controlling these traits, in order to get indirect information on the genetic and physiological basis of maize response to water stress. To this aim, we performed a linkage analysis between the expression of male and female flowering time, anthesis-silking interval (ASI), plant height and molecular markers. The experiment was carried out under two environmental conditions, well-watered and water-stressed, on a maize population of 142 recombinant inbred lines obtained by selfing the F1 between lines B73 and H99 and genotyped by RFLP, microsatellites (SSR) and AFLP markers, for a total of 153 loci. Linkage analysis revealed that, for male flowering time and plant height, most of the QTLs detected were the same under control and stress conditions. In contrast, with respect to female flowering time and ASI diverse QTLs appeared to be expressed either under control conditions or under stress. All of the QTLs conferring tolerance to drought were located in a different chromosome position as compared to the map position of the factors controlling the trait per se. This suggests that plant tolerance, in its different components, is not attributable to the presence of favourable allelic combinations controlling the trait but is based on physiological characteristics not directly associated with the control of the character. 相似文献
5.
Genetic variation at bx1 controls DIMBOA content in maize 总被引:1,自引:0,他引:1
Ana Butrón Y. C. Chen G. E. Rottinghaus M. D. McMullen 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2010,120(4):721-734
The main hydroxamic acid in maize (Zea mays L.) is 2-4-dihydroxy-7-methoxy-1,4-benzoxazin-3-one (DIMBOA). DIMBOA confers resistance to leaf-feeding by several corn borers.
Most genes involved in the DIMBOA metabolic pathway are located on the short arm of chromosome 4, and quantitative trait loci
(QTLs) involved in maize resistance to leaf-feeding by corn borers have been localized to that region. However, the low resolution
of QTL linkage mapping does not allow convincing proof that genetic variation at bx loci was responsible for the variability for resistance. This study addressed the following objectives: to determine the
QTLs involved in DIMBOA synthesis across genetically divergent maize inbreds using eight RIL families from the nested association
mapping population, to check the stability of QTLs for DIMBOA content across years by evaluating two of those RIL families
in 2 years, and to test the involvement of bx1 by performing association mapping with a panel of 281 diverse inbred lines. QTLs were stable across different environments.
A genetic model including eight markers explained approximately 34% of phenotypic variability across eight RIL families and
the position of the largest QTL co-localizes with the majority of structural genes of the DIMBOA pathway. Candidate association
analysis determined that sequence polymorphisms at bx1 greatly affects variation of DIMBOA content in a diverse panel of maize inbreds, but the specific causal polymorphism or
polymorphisms responsible for the QTL detected in the region 4.01 were not identified. This result may be because the causal
polymorphism(s) were not sequenced, identity is masked by linkage disequilibrium, adjustments for population structure reduce
significance of causal polymorphisms or multiple causal polymorphisms affecting bx1 segregate among inbred lines. 相似文献
6.
Background
The harvest index for many crops can be improved through introduction of dwarf stature to increase lodging resistance, combined with early maturity. The inbred line Shen5003 has been widely used in maize breeding in China as a key donor line for the dwarf trait. Also, one major quantitative trait locus (QTL) controlling plant height has been identified in bin 5.05–5.06, across several maize bi-parental populations. With the progress of publicly available maize genome sequence, the objective of this work was to identify the candidate genes that affect plant height among Chinese maize inbred lines with genome wide association studies (GWAS).Methods and Findings
A total of 284 maize inbred lines were genotyped using over 55,000 evenly spaced SNPs, from which a set of 41,101 SNPs were filtered with stringent quality control for further data analysis. With the population structure controlled in a mixed linear model (MLM) implemented with the software TASSEL, we carried out a genome-wide association study (GWAS) for plant height. A total of 204 SNPs (P≤0.0001) and 105 genomic loci harboring coding regions were identified. Four loci containing genes associated with gibberellin (GA), auxin, and epigenetic pathways may be involved in natural variation that led to a dwarf phenotype in elite maize inbred lines. Among them, a favorable allele for dwarfing on chromosome 5 (SNP PZE-105115518) was also identified in six Shen5003 derivatives.Conclusions
The fact that a large number of previously identified dwarf genes are missing from our study highlights the discovery of the consistently significant association of the gene harboring the SNP PZE-105115518 with plant height (P = 8.91e-10) and its confirmation in the Shen5003 introgression lines. Results from this study suggest that, in the maize breeding schema in China, specific alleles were selected, that have played important roles in maize production. 相似文献7.
Genetic mapping in maize with hybrid progeny across testers and generations: plant height and flowering 总被引:9,自引:0,他引:9
D. F. Austin M. Lee L. R. Veldboom 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2001,102(1):163-176
DNA markers were used to identify quantitative trait loci (QTLs) for plant height, ear height, and three flowering traits
in hybrid progeny of two generations (F2:3, F6:8) of lines from a Mo17×H99 maize population. For both generations, testcross (TC) progeny were developed by crossing the lines
to three inbred testers (B91, A632, B73). The hybrid progeny from the two generations were evaluated at the same locations
but in different years as per an early generation testing program. QTLs were identified within each TC population and for
mean testcross (MTC) performance. Overall, more QTLs were detected in the F6:8 than the F2:3 generation. Totalled over all five traits, 41 (B91) to 69% (B73) of the QTLs for tester effects and 67% of the QTLs for MTC
detected in the F2:3 generation were verified in the F6:8 generation. Although differences in relative rank of the QTL effects across generations were observed, especially for the
flowering traits, parental contributions were nearly always consistent. Several (8–11) QTLs were identified with effects for
all three tester populations and for all traits except the anthesis-silk interval, which had only two such regions. Over all
five traits, previous evaluations in this population identified 26 QTLs with consistent effects for two (F2:3, F6:8) inbred-progeny evaluations, and 20 (77%) were also associated with MTC in at least one of the generations evaluated herein.
In all instances of common inbred and TC QTLs, parental contributions were the same.
Received: 26 November 1999 / Accepted: 18 April 2000 相似文献
8.
Shuhui Xu Zefeng Yang Enying Zhang Ying Jiang Liang Pan Qing Chen Zhengwen Xie Chenwu Xu 《PloS one》2014,9(8)
Cereal Brittle1 protein has been demonstrated to be involved in the ADP-Glc transport into endosperm plastids, and plays vital roles in the biosynthesis of starch. In this study, the genomic sequences of the ZmBT1 gene in 80 elite maize inbred lines were obtained, and the nucleotide polymorphisms and haplotype diversity were detected. A total of 30 variants, including 22 SNPs and 8 indels, were detected from the full sequences of this gene. Among these polymorphic sites, 9 SNPs and 2 indels were found to be located in the coding region. The polymorphisms of CDS sequences classified the maize ZmBT1 gene into 6 haplotypes, which encode 6 different ZmBT1 proteins. Neutrality tests revealed a decrease in population size and/or balancing selection on the maize ZmBT1 locus. To detect the association between sequence variations of this gene and the starch physicochemical properties, 7 pasting and 4 gelatinization traits of starch were measured for the tested inbred lines using rapid visco analyzer (RVA) and differential scanning calorimeter (DSC), respectively. The result of association analysis revealed that an indel in the coding region was significantly associated with the phenotypic variation of starch gelatinization enthalpy. 相似文献
9.
Menkir A Kling JG Badu-Apraku B Ingelbrecht I 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2005,110(6):1145-1153
Striga-resistant maize inbred lines are of interest to maize breeding programs in the savannas of Africa where the parasitic weed is endemic and causes severe yield losses in tropical maize. Assessment of the genetic diversity of such inbred lines is useful for their systematic and efficient use in a breeding program. Diversity analysis of 41 Striga-resistant maize inbred lines was conducted using amplified fragment length polymorphism (AFLP) and simple sequence repeat (SSR) markers to examine the genetic relationships among these lines and to determine the level of genetic diversity that exists within and between their source populations. The two marker systems generated 262 and 101 polymorphic fragments, respectively. Genetic similarity (GS) values among all possible pairs of inbred lines varied from 0.45 to 0.95, with a mean of 0.61±0.002 for AFLPs, and from 0.21 to 0.92, with a mean of 0.48±0.003, for SSRs. The inbred lines from each source population exhibited a broad range of GS values with the two types of markers. Both AFLPs and SSRs revealed similar levels of within population genetic variation for all source populations. Cluster and principal component analysis of GS estimates with the two markers revealed clear differentiation of the Striga-resistant inbred lines into groups according to their source populations. There was clear separation between early- and late-maturing Striga-resistant inbred lines. Considering the paucity of germplasm with good levels of resistance to Striga in maize, the broad genetic diversity detected within and among source populations demonstrates the genetic potential that exists to improve maize for resistance to Striga. 相似文献
10.
Ellis MH Rebetzke GJ Azanza F Richards RA Spielmeyer W 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2005,111(3):423-430
Opportunities exist for replacing reduced height (Rht) genes Rht-B1b and Rht-D1b with alternative dwarfing genes for bread wheat improvement. In this study, the chromosomal locations of several height-reducing genes were determined by screening populations of recombinant inbred lines or doubled haploid lines varying for plant height with microsatellite markers. Linked markers were found for Rht5 (on chromosome 3BS), Rht12 (5AL) and Rht13 (7BS), which accounted for most of the phenotypic variance in height in the respective populations. Large height differences between genotypes (up to 43 cm) indicated linkage to major height-reducing genes. Rht4 was associated with molecular markers on chromosome 2BL, accounting for up to 30% of the variance in height. Confirming previous studies, Rht8 was linked to markers on chromosome 2DS, whereas a population varying for Rht9 revealed a region with a small but significant height effect on chromosome 5AL. The height-reducing effect of these dwarfing genes was repeatable across a range of environments. The molecular markers developed in this study will be useful for marker-assisted selection of alternative height-reducing genes, and to better understand the effects of different Rht genes on wheat growth and agronomic performance. 相似文献
11.
Genetic analysis of photoperiod sensitivity in a tropical by temperate maize recombinant inbred population using molecular markers 总被引:2,自引:0,他引:2
Wang CL Cheng FF Sun ZH Tang JH Wu LC Ku LX Chen YH 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2008,117(7):1129-1139
Photoperiod sensitivity is an important consideration in maize cultivation. Flowering time is affected by photoperiod and
sensitivity to it limits the potential for successful exchange of germplasm across different latitudes. For resolving the
genetic basis of photoperiod sensitivity in maize, a set of 207 recombinant inbred lines derived from a temperate and tropical
inbred line cross was evaluated for 2 years in a long-day and short-day environment. Genetic linkage maps were constructed
using 237 SSR markers with a total length 1,974.3 cM, and an average space between two makers of 8.33 cM. Twenty-nine QTL
were detected for the five measured photoperiod sensitivity traits using composite interval mapping and multiple interval
mapping. QTL for flowering time, plant height and leaf number, under long-day conditions, were found clustered on chromosome
10, while QTL for short-day conditions resided on chromosome 3. The QTL in the bin 10.04 region of chromosome 10 were detected
associated with photoperiod sensitivity and related traits during long days. These results indicated that this region might
contain an important photoperiod sensitivity element. 相似文献
12.
Genetic resolution and verification of quantitative trait loci for flowering and plant height with recombinant inbred lines of maize. 总被引:10,自引:0,他引:10
Recombinant inbred (RI) lines offer several advantages for detecting quantitative trait loci (QTLs), including increased precision of trait measurements, power for detection of additive effects, and resolution of linked QTLs. This study was conducted to detect and characterize QTLs in maize for flowering and plant height and to compare QTL detection in an early (F2:3) generation of the same population. One hundred and eighty-six RIs from a cross between inbred lines Mo17 and H99 were evaluated in a replicated field experiment and analyzed at 101 loci detected by restriction fragment length polymorphisms. QTLs were identified by single-factor analysis of variance. A total of 59 QTLs were detected for plant height, ear height, top height, anthesis, silk emergence, and anthesis to silk interval. Individual QTLs explained 2.2-15.4% of trait variation, and multiple models including all QTLs detected for a trait explained up to 52.5% of the phenotypic variation. Comparison of QTLs detected with 150 F2:3 lines from the same population indicated that 16 (70%) of the 23 F2:3 QTLs were also observed in the F6:7 generation. Parental effects were consistent across generations. At 14 of the 16 QTLs detected in both generations, genetic effects were smaller in the F6:7. Also, some QTLs detected in the F2:3 were resolved into multiple linked QTLs in the F6:7, indicating the additional power of RI populations for mapping, with important implications for marker-assisted selection as well as map-based cloning of QTLs. Key words : Zea mays, RFLP, plant breeding, genetics, recombination. 相似文献
13.
Elena Cassani Edoardo Bertolini Francesco Cerino Badone Michela Landoni Dario Gavina Alberto Sirizzotti Roberto Pilu 《Molecular breeding : new strategies in plant improvement》2009,24(4):375-385
The “green revolution” involving mainly wheat and rice was based on the use by breeders of semidominant mutations involved
in the signal transduction pathway of Gibberellin (GA). In particular, mutations in the Reduced height (Rht) gene of wheat have been used to reduce plant height and consequently to avoid storm damage and lodging. These genes have
been cloned and they encode for DELLA proteins which contain an N-terminal DELLA and a VHYNP domain essential for GA-dependent
degradation of these proteins. In maize several mutations have been isolated which affect gibberellin biosynthesis and perception
and in particular, mutations in Dwarf8 (D8) gene cause a severe dwarfing phenotype. D8 gene has been identified as an orthologue of Rht (Reduced height), Slr1(Slender rice 1) and Gibberellic Acid Insensitive (GAI) genes, this latter is a negative regulator of GA response in Arabidopsis. In this work, for the first time, we isolated and characterized a single amino acid insertion in the VHYNP domain of D8 maize gene causing the appearance of a dominant dwarf mutation. This spontaneous mutation, named D8-1023, showed a phenotype which is less severe in comparison with the other D8 mutants previously isolated which have modifications
in the DELLA domain. This mutant appears to be an useful tool either to study the mechanism of GA-modulated growth in plants
or to lower the height of maize tropical germplasm for breeding purposes. 相似文献
14.
Stich B Melchinger AE Piepho HP Heckenberger M Maurer HP Reif JC 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2006,113(6):1121-1130
Association mapping holds great promise for the detection of quantitative trait loci (QTL) in plant breeding populations. The main objectives of this study were to (1) adapt the quantitative pedigree disequilibrium test to typical pedigrees of inbred lines produced in plant breeding programs, (2) compare the newly developed quantitative inbred pedigree disequilibrium test (QIPDT) with the commonly employed logistic regression ratio test (LRRT), with respect to the power and type I error rate of QTL detection, and (3) demonstrate the use of the QIPDT by applying it to flowering data of European elite maize inbreds. QIPDT and LRRT were compared based on computer simulations modeling 55 years of hybrid maize breeding in Central Europe. Furthermore, we applied QIPDT to a cross-section of 49 European elite maize inbred lines genotyped with 722 amplified fragment length polymorphism markers and phenotyped in four environments for days to anthesis. Compared to LRRT, the power to detect QTL was higher with QIPDT when using data collected routinely in plant breeding programs. Application of QIPDT to the 49 European maize inbreds resulted in a significant (P < 0.05) association located at a position for which a consensus QTL was detected in a previous study. The results of our study suggested that QIPDT is a promising QTL detection method for data collected routinely in plant breeding programs. 相似文献
15.
A large nested association mapping population for breeding and quantitative trait locus mapping in Ethiopian durum wheat 总被引:2,自引:0,他引:2
Yosef G. Kidane Cherinet A. Gesesse Bogale N. Hailemariam Ermias A. Desta Dejene K. Mengistu Carlo Fadda Mario Enrico P Matteo Dell'Acqua 《Plant biotechnology journal》2019,17(7):1380-1393
The Ethiopian plateau hosts thousands of durum wheat (Triticum turgidum subsp. durum) farmer varieties (FV) with high adaptability and breeding potential. To harness their unique allelic diversity, we produced a large nested association mapping (NAM) population intercrossing fifty Ethiopian FVs with an international elite durum wheat variety (Asassa). The Ethiopian NAM population (EtNAM) is composed of fifty interconnected bi‐parental families, totalling 6280 recombinant inbred lines (RILs) that represent both a powerful quantitative trait loci (QTL) mapping tool, and a large pre‐breeding panel. Here, we discuss the molecular and phenotypic diversity of the EtNAM founder lines, then we use an array featuring 13 000 single nucleotide polymorphisms (SNPs) to characterize a subset of 1200 EtNAM RILs from 12 families. Finally, we test the usefulness of the population by mapping phenology traits and plant height using a genome wide association (GWA) approach. EtNAM RILs showed high allelic variation and a genetic makeup combining genetic diversity from Ethiopian FVs with the international durum wheat allele pool. EtNAM SNP data were projected on the fully sequenced AB genome of wild emmer wheat, and were used to estimate pairwise linkage disequilibrium (LD) measures that reported an LD decay distance of 7.4 Mb on average, and balanced founder contributions across EtNAM families. GWA analyses identified 11 genomic loci individually affecting up to 3 days in flowering time and more than 1.6 cm in height. We argue that the EtNAM is a powerful tool to support the production of new durum wheat varieties targeting local and global agriculture. 相似文献
16.
Prioul S Frankewitz A Deniot G Morin G Baranger A 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2004,108(7):1322-1334
The inheritance of resistance to Ascochyta blight, an economically important foliar disease of field pea (Pisum sativum L.) worldwide, was investigated. Breeding resistant pea varieties to this disease, caused by Mycosphaerella pinodes, is difficult due to the availability of only partial resistance. We mapped and characterized quantitative trait loci (QTLs) for resistance to M. pinodes in pea. A population of 135 recombinant inbred lines (RILs), derived from the cross between DP (partially resistant) and JI296 (susceptible), was genotyped with morphological, RAPD, SSR and STS markers. A genetic map was elaborated, comprising 206 markers distributed over eight linkage groups and covering 1,061 cM. The RILs were assessed under growth chamber and field conditions at the seedling and adult plant stages, respectively. Six QTLs were detected at the seedling stage, which together explained up to 74% of the variance. Ten QTLs were identified at the adult plant stage in the field, and together these explained 56.6–67.1% of the variance, depending on the resistance criteria and the organ considered. Four QTLs were detected under both growth chamber and field conditions, suggesting they were not plant-stage dependent. Three QTLs for flowering date and three QTLs for plant height were also identified in the RIL population, some of which co-located with QTLs for resistance. The relationship between QTLs for resistance to M. pinodes, plant height and flowering date is discussed.Communicated by H.C. Becker 相似文献
17.
Jian Liu Jingtao Qu Cong Yang Dengguo Tang Jingwei Li Hai Lan Tingzhao Rong 《BMC genomics》2015,16(1)
Background
Insertions and deletions (indels) are the most abundant form of structural variation in all genomes. Indels have been increasingly recognized as an important source of molecular markers due to high-density occurrence, cost-effectiveness, and ease of genotyping. Coupled with developments in bioinformatics, next-generation sequencing (NGS) platforms enable the discovery of millions of indel polymorphisms by comparing the whole genome sequences of individuals within a species.Results
A total of 1,973,746 unique indels were identified in 345 maize genomes, with an overall density of 958.79 indels/Mbp, and an average allele number of 2.76, ranging from 2 to 107. There were 264,214 indels with polymorphism information content (PIC) values greater than or equal to 0.5, accounting for 13.39 % of overall indels. Of these highly polymorphic indels, we designed primer pairs for 83,481 and 29,403 indels with major allele differences (i.e. the size difference between the most and second most frequent alleles) greater than or equal to 3 and 8 bp, respectively, based on the differing resolution capabilities of gel electrophoresis. The accuracy of our indel markers was experimentally validated, and among 100 indel markers, average accuracy was approximately 90 %. In addition, we also validated the polymorphism of the indel markers. Of 100 highly polymorphic indel markers, all had polymorphisms with average PIC values of 0.54.Conclusions
The maize genome is rich in indel polymorphisms. Intriguingly, the level of polymorphism in genic regions of the maize genome was higher than that in intergenic regions. The polymorphic indel markers developed from this study may enhance the efficiency of genetic research and marker-assisted breeding in maize.Electronic supplementary material
The online version of this article (doi:10.1186/s12864-015-1797-5) contains supplementary material, which is available to authorized users. 相似文献18.
Elena Cadic Marie Coque Felicity Vear Bruno Grezes-Besset Jerôme Pauquet Joël Piquemal Yannick Lippi Philippe Blanchard Michel Romestant Nicolas Pouilly David Rengel Jerôme Gouzy Nicolas Langlade Brigitte Mangin Patrick Vincourt 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2013,126(5):1337-1356
Association mapping and linkage mapping were used to identify quantitative trait loci (QTL) and/or causative mutations involved in the control of flowering time in cultivated sunflower Helianthus annuus. A panel of 384 inbred lines was phenotyped through testcrosses with two tester inbred lines across 15 location × year combinations. A recombinant inbred line (RIL) population comprising 273 lines was phenotyped both per se and through testcrosses with one or two testers in 16 location × year combinations. In the association mapping approach, kinship estimation using 5,923 single nucleotide polymorphisms was found to be the best covariate to correct for effects of panel structure. Linkage disequilibrium decay ranged from 0.08 to 0.26 cM for a threshold of 0.20, after correcting for structure effects, depending on the linkage group (LG) and the ancestry of inbred lines. A possible hitchhiking effect is hypothesized for LG10 and LG08. A total of 11 regions across 10 LGs were found to be associated with flowering time, and QTLs were mapped on 11 LGs in the RIL population. Whereas eight regions were demonstrated to be common between the two approaches, the linkage disequilibrium approach did not detect a documented QTL that was confirmed using the linkage mapping approach. 相似文献
19.
20.
Characterization of a global germplasm collection and its potential utilization for analysis of complex quantitative traits in maize 总被引:4,自引:0,他引:4
Xiaohong Yang Shibin Gao Shutu Xu Zuxin Zhang Boddupalli M. Prasanna Lin Li Jiansheng Li Jianbing Yan 《Molecular breeding : new strategies in plant improvement》2011,28(4):511-526
Association mapping is a powerful approach for exploring the molecular basis of phenotypic variations in plants. A maize (Zea mays L.) association mapping panel including 527 inbred lines with tropical, subtropical and temperate backgrounds, representing
the global maize diversity, was genotyped using 1,536 single nucleotide polymorphisms (SNPs). In total, 926 SNPs with minor
allele frequencies of ≥0.1 were used to estimate the pattern of genetic diversity and relatedness among individuals. The analysis
revealed broad phenotypic diversity and complex genetic relatedness in the maize panel. Two different Bayesian approaches
identified three specific subpopulations, which were then reconfirmed by principal component analysis (PCA) and tree-based
analyses. Marker–trait associations were performed to assess the suitability of different models for false-positive correction
by population structure (Q matrix/PCA) and familial kinship (K matrix) alone or in combination in this panel. The K, Q + K
and PCA + K models could reduce the false positives, and the Q + K model performed slightly better for flowering time, ear
height and ear diameter. Our findings suggest that this maize panel is suitable for association mapping in order to understand
the relationship between genotypic and phenotypic variations for agriculturally complex quantitative traits using optimal
statistical methods. 相似文献