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1.
A linkage map of rye 总被引:4,自引:0,他引:4
U. Philipp P. Wehling G. Wricke 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1994,88(2):243-248
A linkage map of rye (Secale cereale L.) is presented which comprises 60 loci including RFLPs, RAPDs, isozyme, morphological and physiological markers. The genetics and linkage relationships of these markers were investigated in several inbred lines of rye. For the RFLP mapping a genomic library of PstI-digested DNA was constructed from which 50 size-selected clones were analysed. The portion of single-copy and multi-copy DNA and the frequency of polymorphic DNA was determined. The markers are unequally distributed over the seven chromosomes of rye. Many of them exhibit a distorted segregation. The main region of deviating segregation ratios could be localized near the self-incompatibility loci. 相似文献
2.
Y. Loarce G. Hueros E. Ferrer 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1996,93(7):1112-1118
A genetic map of six chromosomes of rye, (all of the rye chromosomes except for 2R), was constructed using 77 RFLP and 12 RAPD markers. The map was developed using an F2 population of 54 plants from a cross between two inbred lines. A rye genomic library was constructed as a source of clones for RFLP mapping. Comparisons were made between the rye map and other rye and wheat maps by including additional probes previously mapped in those species. These comparisons allowed (1) chromosome arm orientation to the linkage groups to be given, (2) the corroboration of several evolutionary translocations between rye chromosomes and homoeologous chromosomes of wheat; (3) an increase in the number of available markers for target regions of rye that show colinearity with wheat. Inconsistencies in the location of markers between the wheat and rye maps were mostly detected by multi-copy probes. 相似文献
3.
Stojałowski S Myśków B Milczarski P Masojć P 《Cellular & molecular biology letters》2009,14(2):190-198
Four F2 mapping populations derived from crosses between rye inbred lines DS2×RXL10, 541×Ot1-3, S120×S76 and 544×Ot0-20 were used
to develop a consensus map of chromosome 6R. Thirteen marker loci that were polymorphic in more than one mapping population
constituted the basis for the alignment of the four maps using the JoinMap v. 3.0 software package. The consensus map consists
of 104 molecular marker loci including RFLPs, RAPDs, AFLPs, SSRs, ISSRs, SCARs, STSs and isozymes. The average distance between
the marker loci is 1.3 cM, and the total map length is 135.5 cM. This consensus map may be used as a source of molecular markers
for the rapid development of new maps of chromosome 6R in any mapping population. 相似文献
4.
P. Garcia Dr. M. Pérez de la Vega C. Benito 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1982,61(4):341-351
Summary Genetic analyses were conducted on peroxidase of the embryo and endosperm of seeds of one open pollinated and six inbred lines of cultivated rye (Secale cereale L.), and one line of Secale vavilovii Grossh. The analyses of the individual parts of the S. cereale seed yield a total of 14 peroxidase isozymes. Isozymes m, a, b, c, d, e, f and g (in order from faster to slower migration) were found in the embryo plus scutellum, while isozymes 1, 2, 3, 4, 5 and 6 (also from faster to slower migration) were peculiar of the endosperm. S. vavilovii has isozymes m, c1, d, e, f and g in its embryo plus scutellum, and isozyme 2 in the endosperm. Segregation data indicated that at least 13 different loci would be controlling the peroxidase of S. cereale. Isozymes a and b are controlled by alleles of the same locus, all the other loci have one active and dominant allele coding for one isozyme, and other null and recessive allele. The estimation of linkage relationships shows that five endosperm loci are linked, and tentative maps are shown. A possible dosage effect and the existence of controlling gene(s) for endosperm isozyme 4 is reported. All these data and the high frequency of null alleles found are discussed in relation to recent reports. 相似文献
5.
C. Benito C. Zaragoza F. J. Gallego A. de la Peña A. M. Figueiras 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1991,82(1):112-116
Summary The segregation of isozymic loci for leaf peroxidases (L2Per) has been investigated in backcrosses and F2 offspring of rye lines having purple seeds (Ps) and monstrosum ears (mo). The Ps, L2Per-3b, mo, and L2Per-2 loci were linked. The Ps and mo loci have been previously located on the 2R chromosome, and the L2Per-3b and L2Per-2 loci have been located on the 2RS chromosome arm. The results favor the gene order Ps ... L2Per-3b ... mo ... L2Per-2 or Ps ... mo... L2Per-3b ... L2Per-2. The position of the loci relative to the centromere is still not known, but the obtained results suggest that the mo locus could be located on the 2RS chromosome arm. On the basis of previously reported linkage groups, the most probable arrangement of the loci located on chromosome 2R is: dw2 ... Ps ... (L2Per-3a ... L2Per-3b ... mo) ... L2Per-2. It has not been possible to know the position of L2Per-4 loci (also located on 2RS chromosome arm) relative to L2Per-3a and L2Per-3b loci. 相似文献
6.
A consensus linkage map of rye (Secale cereale L.) including 374 RFLPs, 24 isozymes and 15 gene loci
A. Börner V. Korzun 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1998,97(8):1279-1288
Consensus linkage maps were constructed for all seven rye chromosomes using 12 basic RFLP maps. The maps presented contain
a total of 413 markers. The number of markers per chromosome varies from 41 (chromosome 3R) to 83 (chromosome 1R). In addition
to 374 RFLP and 24 isozyme markers 15 gene loci were incorporated, determining the traits reduced plant height, self fertility,
male sterility restoration, vernalization response, resistance against powdery mildew, chlorophyll deficiency, hairy leaf
sheath, hairy peduncle, waxy endosperm, waxless plant and absence of ligules. The maps presented allow the selection of markers
for the fine mapping of certain regions of the rye genome. In terms of the known chromosomal rearrangements within the Triticeae its utilization can also be extended for mapping in wheat and barley.
Received: 13 February 1998 / Accepted: 26 May 1998 相似文献
7.
We have obtained one plant regenerated from rye tissue culture which showed a high percentage of polyembryonic seeds in its progeny. The mutation inducing the development of extra embryos is also influencing erroneous cell division, mitosis and meiosis. The genetic analysis indicated that the aptitude for polyembryonic seed formation is a heritable trait controlled by a dominant gene. However, for expression of the phenotype the female parent should have a specific cytoplasm. 相似文献
8.
F. Vaquero L. Rebordinos F. J. Vences M. Pérez de la Vega 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1990,80(1):88-94
Summary The genetics and linkage relationships of several isozymatic and morphological markers have been investigated in different cultivars of rye (Secale cereale L.). The inheritance and the variability among cultivars of three new isozymatic zones are described: GOT2 and LAP, each of them under the control of a two-allele single locus, namely Got2 and Lap, respectively; and 6PGD1 controlled by two loci, 6Pgd1a and 6Pgd1b, which have alleles in common. Four linkage groups have been found: Acp2-Acp3, Got3-Mdh2-Lper4, Mdh1-6Pgd2-Pgi2, and Pgm-Eper2-[Eper1-Eper3]. The assignment of these four groups to the chromosomes 7R, 3R, 1R, and 4R is discussed. 相似文献
9.
J. M. Carrillo J. F. Vázquez J. Orellana 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1992,84(1-2):237-241
Summary The progenies of two different rye test-crosses were analyzed for secalin proteins by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) using unreduced and reduced aqueous ethanol extracts. Segregation for two high-molecular-weight secalin bands (Glu-R1 or Sec3), one -secalin band (Gli-R1 or Sec-1), two 40K -secalin bands (Gli-R1 or Sec1) and two -type secalin bands (new locus) were studied. One recombinant between - and -secalins was found in one test-cross. The new locus, designated Gli-R3 or Sec-4, was mapped between Glu-R1 and Gli-R1, more displaced towards Gli-R1. In test-cross 1 recombination between Glu-R1 and Gli-R3 was 33.80±3.22%, and between Gli-R3 and Gli-R1, 12.04±2.21%. In the other test-cross the map distances were relatively similar but smaller, likely due to less recombination within two different species of Secale. Genes coding for 40K -secalins at Gli-R1 were likely proximal to the centromere with respect to genes coding for -secalins at the same complex locus. 相似文献
10.
F. Hoffmann G. Wenzel 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1981,60(3):129-133
Summary From a total of 138 green androgenetic rye lines, 25 were fertilized and examined in field tests: 7 were heterozygous and 18 were homozygous. Of the homozygotes, 4 turned out to be selfincompatible, while 14 set seed after selfing. Four characters were analyzed in detail: 100 kernel weight, plant height, ear length, and alkylresorcinol content. Here we present the first approach in prescreening selfcompatible androgenetic doubled-haploid rye plants with the single grain procedure. The usefulness of this method was confirmed by quantitative resorcinol determination in the following generation. Furthermore, it was demonstrated that all the homologues of the alkylresorcinol were equally reduced. For all characters the means of the different anther derived lines exceeded the means of the controls in both directions, to the positive as well as to the negative side. The incorporation of such a haploid breeding step into breeding programs is discussed.Dedicated to Professor Dr. G. Melchers, on the occasion of his 75th birthday, who initiated this work by forming the project-groups Haploide in der Pflanzenzüchtung 相似文献
11.
P. Wehling G. Schmidt-Stohn 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1984,67(2-3):149-153
Summary Genetic analysis of esterase polymorphism in rye inbred lines with isoelectric focusing in polyacrylamide flat gels yielded evidence for the existence of at least ten esterase loci, Est 1–Est 10. The loci can be attributed to four different linkage groups (Est 1/ Est 2/Est 3/Est 5/Est 6/Est 7), (Est 4), (Est 8/Est 9), and (Est 10). Loci Est 5/Est 6/Est 7 and Est 8/Est 9, respectively, are tightly linked with a maximum recombination frequency of 0.2% and can therefore be regarded as compound loci which possibly originated in tandem duplications. 相似文献
12.
J. N. de Vries J. Sybenga 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1989,78(6):889-896
Summary Meiosis of four telocentric-tertiary compensating trisomics of rye (Secale cereale L.) was studied with the purpose of evaluating their suitability for use in maintaining genic male-sterile lines applied in hybrid varieties. They had been constructed from four different reciprocal translocations and three different telocentrics. In one trisomic a slight, but significant tendency was demonstrated for preferential pairing of the two normal chromosomes associated with the compensating complex. This promotes the desired segregation into one normal and one compensating karyotype. In all trisomies, however, too high a frequency of failure of chiasma formation in a critical segment of the complex was evident. This is correlated with the ease of recovery of the trisomics, but results in undesired segregational products. Interstitial chiasmata leading to the formation of branched configurations were also present, more in some trisomics than in others. These also result in undesired segregations. The behaviour at meiosis was so closely correlated with the length of the chromosome segments involved that a prediction of the most favourable combination of telocentric and translocation can be made. The telocentric should be large, the corresponding translocated segment large and the interstitial segment small. The non-translocated arm of the translocated chromosome should be large and the second translocated segment small. The combinations of translocations and telocentrics had not been selected for these criteria and did not meet the requirements for practical application. 相似文献
13.
Chwedorzewska KJ Bednarek PT Lewandowska R Krajewski P Puchalski J 《Cellular & molecular biology letters》2006,11(3):338-347
The aim of this study was to identify genetic changes in rye seeds induced by natural ageing during long-term storage and
consecutive regeneration cycles under gene bank conditions. Genomic DNA from four rye samples varying in their initial viability
after one and three cycles of reproduction was analyzed by AFLP (amplified fragment length polymorphism) fingerprinting. Seven
EcoRI/MseI primer combinations defined 663 fragments, and seven PstI/MseI primer combinations defined 551 fragments. The variation in the frequency of the seventy-four EcoRI/MseI bands was statistically significant between samples. These changes could be attributed to genetic changes occurring during
storage and regeneration. However, the PstI/MseI fragments appeared to be uninfluenced by seed ageing, regeneration and propagation. A combined Principle Coordinate Analysis
revealed differences between samples with different initial viability. We showed that materials with low initial viability
differ in their response from highly viable ones, and that the changes exhibited in the former case are preserved through
regeneration cycles. 相似文献
14.
A. M. Figueiras M. T. González-Jaén C. Benito 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1987,73(5):683-689
Summary Genetic analyses were conducted on alkaline phosphatases of the endosperm of dry kernels and leaf acid phosphatases in four open pollinated and one inbred line of cultivated rye (Secale cereale L.). A total of seven alkaline phosphatase isozymes were observed occurring at variable frequencies in the different cultivars analyzed. We propose that at least five loci control the alkaline phosphatases of rye endosperm — Alph-1, Alph-2, Alph-3, Alph-4 and Alph-5 — all of which have monomeric behaviour. The leaf acid phosphatases are controlled by one locus and have a dimeric quaternary structure. All loci coding for alkaline phosphatase isozymes showed one active, dominant allele and one null, recessive allele, except for the locus Alph-3 which showed two active, dominant alleles and one null, recessive one. The linkage analyses suggest the existence of two linkage groups for alkaline phosphatases: one of them would contain Alph-2, Alph-4, Alph-5 and the locus/loci coding isozymes 6 and 7. This linkage group is located in the 7RS chromosome arm. The other group would include Alph-1 and Alph-3 loci, being located in the 1RL chromosome arm. Leaf acid phosphatases have been previously located in the 7RL chromosome arm. Our data also support an independent relationship between loci controlling the endosperm alkaline phosphatases and leaf acid phosphatases. 相似文献
15.
V. Lombard R. Delourme 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2001,103(4):491-507
A framework consensus map for rapeseed (Brassica napus L.) was constructed from the integration of three DH mapping populations derived from crosses between or within spring- and
winter-type parents. Several sources of genetic markers were used: isozymes, RFLPs, RAPDs, and AFLPs. A total of 992 different
markers were mapped to at least one population, of which 540 were included in the consensus map and 253 were common to at
least two populations. Markers were distributed over 19 linkage groups, thus reflecting the basic chromosome number of rapeseed
and covered 2,429 cM, which was in the mean confidence-interval estimates of genome length (2,127–2,480) cM. Markers were
evenly spaced on the entire genome even if, for several linkage groups, both RAPD and AFLP markers were not uniformly distributed.
In the population resulting from a cross between two spring lines, a higher recombination rate was observed and a translocation
was identified. The consensus approach allowed to map a larger number of markers, to obtain a near-complete coverage of the
rapeseed genome, to fill the number of gaps, and to consolidate the linkage groups of the individual maps.
Received: 19 July 2000 / Accepted: 31 October 2000 相似文献
16.
M. Rakoczy-Trojanowska S. Malepszy 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1993,86(4):406-410
Immature inflorescences of ten rye inbred lines (inbred degree S10 and S11) were cultured on solidified MS medium supplemented with 3.0 mg/dm3 of 2,4-D. According to their capability for callus production explants were classified into two groups : responsive (giving weak or intensive callus production) and non responsive (lack of callus formation). After transferring responsive material into hormone-free medium the regeneration of roots or shoots from the intensive growing callus was observed. Consistent differences between lines in the portion of explants with a certain response were found. They were divided into five groups reacting in the same way. Lines with different in-vitro response were crossed in an incomplete diallel. F1, F2 and F3 generations were analyzed and the following conclusions drawn: the ability for plant regeneration from immature inflorescences in rye is determined by numerous loci, has a recessive character, and both callus production and regeneration suppression may be controlled by complementary genes. 相似文献
17.
Construction of an integrated consensus map of the apple genome based on four mapping populations 总被引:2,自引:0,他引:2
A. N’Diaye W. E. Van de Weg L. P. Kodde B. Koller F. Dunemann M. Thiermann S. Tartarini F. Gennari C. E. Durel 《Tree Genetics & Genomes》2008,4(4):727-743
An integrated consensus genetic map for apple was constructed on the basis of segregation data from four genetically connected crosses (C1?=?Discovery × TN10-8, C2?=?Fiesta × Discovery, C3?=?Discovery × Prima, C4?=?Durello di Forli × Fiesta) with a total of 676 individuals using CarthaGene® software. First, integrated female–male maps were built for each population using common female–male simple sequence repeat markers (SSRs). Then, common SSRs over populations were used for the consensus map integration. The integrated consensus map consists of 1,046 markers, of which 159 are SSR markers, distributed over 17 linkage groups reflecting the basic chromosome number of apple. The total length of the integrated consensus map was 1,032 cM with a mean distance between adjacent loci of 1.1 cM. Markers were proportionally distributed over the 17 linkage groups (χ 2?=?16.53, df?=?16, p?=?0.41). A non-uniform marker distribution was observed within all of the linkage groups (LGs). Clustering of markers at the same position (within a 1-cM window) was observed throughout LGs and consisted predominantly of only two to three linked markers. The four integrated female–male maps showed a very good colinearity in marker order for their common markers, except for only two (CH01h01, CH05g03) and three (CH05a02z, NZ02b01, Lap-1) markers on LG17 and LG15, respectively. This integrated consensus map provides a framework for performing quantitative trait locus (QTL) detection in a multi-population design and evaluating the genetic background effect on QTL expression. 相似文献
18.
M. Matos V. Pérez-Flores M. V. Camacho B. Pernaute O. Pinto-Carnide C. Benito 《Molecular breeding : new strategies in plant improvement》2007,20(2):103-115
Aluminium toxicity is a major problem for crop production on acid soils. Rye (Secale cereale L.) has one of the most efficient group of genes for aluminium tolerance, at least, four independent and dominant loci, Alt1, Alt2, Alt3 and Alt4, located on chromosome arms 6RS, 3RS, 4RL and 7RS, have been described. The increasing availability of expressed sequence
tags in rye and related cereals provides a valuable resource of non-anonymous DNA molecular markers. In order to obtain simple
sequence repeat (SSR) markers related with Al tolerance more than 1,199 public accessible rye cDNA sequences from Al-stressed
roots were exploited as a resource for SSR markers development. From a total of 21 S. cereale microsatellite (SCM) loci analysed, 12 were located on chromosomes 1R, 2R, 3R, 4R and 5R, using wheat–rye addition lines
or mapped using a F2 population segregating for Al tolerance. Seven SCM loci were included in a rye map with other SCIM and RAPD markers. Moreover,
14 SCM loci could be associated to proteins with known or unknown function. The possible implications of these sequences in
aluminium tolerance mechanisms are discussed. 相似文献
19.
G. Wricke P. Wehling 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1985,71(2):289-291
Summary Under controlled growth chamber conditions of 30 °C, seed set after selfing is possible in normally self-incompatible rye plants. Within selfed progenies produced by this method, plants homozygous at the peroxidase isozyme locus Prx 7 were crossed to heterozygous individuals. Segregation at the Prx 7 locus in progenies of these crosses provides clear evidence of a close linkage between Prx 7 and one of the two incompatibility loci in rye. A recombination fraction in the range of 0–2% was calculated from the segregation data. In rye, Prx 7 is linked with a phosphoglucoisomerase locus (Pgi). The similarity between the observations in Secale cereale and those made in Lolium perenne is discussed. 相似文献
20.
A method for somatic embryogenesis and fertile green plant regeneration from suspension cell-derived protoplasts of rye (Secale cereale L. cv. Auvinen) was developed. Fast-growing and friable embryogenic calli with a high regeneration capacity were induced from immature rye inflorescences using modified MS medium. These friable embryogenic calli were used for suspension culture initiation in liquid AA medium. A high yield of protoplasts was obtained from suspension cell clumps after 3–5 days of subculture. Isolated protoplasts were cultured in KM8p medium. The frequency of protoplast cell divisions and colony formations in liquid culture medium were similar to those on agarose-solidified medium. Compact embryogenic calli were developed from protoplast-derived microcalli in growth medium mMS. Approximately 7% of the transferred embryogenic calli produced green shoots on N6 regeneration medium. Of 33 green plants, 28 were fertile with normal flowering and seed set. The ratio of green and albino plantlets was 1:4. Rye protoplast-derived green plants showed normal diploid characters as determined by flow cytometer analysis and chromosome counting.Abbreviations 2,4-D 2,4-Dichorophenoxyacetic acid - FDA Fluorescein diacetate - FW Fresh weight - GA3 Gibberellic acid - Kinetin 6-Furfurylaminopurine - IAA Indole-3-acetic acid - NAA -Naphthaleneacetic acid 相似文献