共查询到20条相似文献,搜索用时 21 毫秒
1.
Ueki T Adachi T Kawano S Aoshima M Yamaguchi N Kanamori K Michibata H 《Biochimica et biophysica acta》2003,1626(1-3):43-50
Since the beginning of the last century, it has been known that ascidians accumulate high levels of a transition metal, vanadium, in their blood cells, although the mechanism for this curious biological function remains unknown. Recently, we identified three vanadium-binding proteins (vanabins), previously denoted as vanadium-associated proteins (VAPs) [Zool. Sci. 14 (1997) 37], from the cytoplasm fraction of vanadium-containing blood cells (vanadocytes) of the vanadium-rich ascidian Ascidia sydneiensis samea. Here, we describe the cloning, expression, and analysis of the metal-binding ability of vanabins. Recombinant proteins of two independent but related vanabins, vanabin1 and vanabin2, bound to 10 and 20 vanadium(IV) ions with dissociation constants of 2.1x10(-5) and 2.3x10(-5) M, respectively. The binding of vanadium(IV) to these vanabins was inhibited by the addition of copper(II) ions, but not by magnesium(II) or molybdate(VI) ions. Vanabins are the first proteins reported to show specific binding to vanadium ions; this should provide a clue to resolving the problem regarding the selective accumulation of vanadium in ascidians. 相似文献
2.
Patrick Poucheret René Gross Anne Cadène Michelle Mantéguetti Jean-Jacques Serrano Gérard Ribes Gérard Cros 《Molecular and cellular biochemistry》1995,153(1-2):197-204
We have previously shown that 3 week oral VOSO4 treatment of streptozotocin (STZ, 60 mg/kg)-induced diabetic rats was able to correct diabetes for 13 weeks after treatment withdrawal. In the present study, we investigated whether a short-term (8 days) i.p. VOSO4 treatment was similarly able to reverse the diabetic state. Insulin secretory capacities were assessed at distance of treatment using the isolated pancreas preparation. Seven treatment-groups were performed: high dose VOSO4-treated diabetics (HVD, 1.3 mM/kg/8 days), food-restricted diabetics (FRD, food adjusted to HVD levels), low dose VOSO4-treated diabetes (LVD, 0.06 mM/kg/day), insulin-treated diabetics (ID, dose adjusted to normalize glycaemia) and VOSO4 (0.06 mM/kg/day) + insulin (dose adjusted to normalize glycaemia in the presence of vanadium)-treated diabetics (IVD), in addition to the corresponding untreated non-diabetic controls (C) and diabetics (D). Our results indicate that long-term correction of diabetes (a) can be obtained after an 8 day treatment using i.p. VOSO4 in diabetic animals retaining some degree of pancreatic function, (b) is not obtained with insulin treatment or food restriction although the association of VOSO4 and insulin was found beneficial, (c) can be prolonged in some individuals for at least 4 months, i.e. in conditions such that tissue vanadium concentrations had returned to values close to pre-treatment levels, (d) is associated with improved and in some cases normalized insulin secretion from isolated pancreas. The protective or corrective role of VOSO4 on diabetes-related pancreatic alterations, as well as the potential of the VOSO4-insulin association should be further studied in view of the possible use of vanadium derivatives in the treatment of diabetes. 相似文献
3.
Debbie C. Crans Mohammed Mahroof-Tahir Anastasios D. Keramidas 《Molecular and cellular biochemistry》1995,153(1-2):17-24
The stability of 11 vanadium compounds is tested under physiological conditions and in administration fluids. Several compounds including those currently used as insulin-mimetic agents in animal and human studies are stable upon dissolution in distilled water but lack such stability in distilled water at pH7. Complex lability may result in decomposition at neutral pH and thus may compromise the effectiveness of these compounds as therapeutic agents; Even well characterized vanadium compounds are surprisingly labile. Sufficiently stable complexes such as the VEDTA complex will only slowly reduce, however, none of the vanadium compounds currently used as insulin-mimetic agents show the high stability of the VEDTA complex. Both the bis(maltolato)oxovanadium(IV) and peroxovanadium complexes extend the insulin-mimetic action of vanadate in reducing cellular environments probably by increased lifetimes under physiological conditions and/or by decomposing to other insulin mimetic compounds. For example, treatment with two equivalents of glutathione or other thiols the (dipicolinato)peroxovanadate(V) forms 9dipicolinato)oxovanadate(V) and vanadate, which are both insulin-mimetic vanadium(V) compounds and can continue to act. The reactivity of vanadate under physiological conditions effects a multitude of biological responses. Other vanadium complexes may mimic insulin but not induce similar responses if the vanadate formation is blocked or reduced. We conclude that three properties, stability, lability and redox chemistry are critical to prolong the half-life of the insulin-mimetic form of vanadium compounds under physiological conditions and should all be considered in development of vanadium-based oral insulin-mimetic agents.Abbreviations ADP
adenosine 5-diphosphate
- ATP
adenosine 5-triphosphate
- ADP-V
adenosine 5-diphosphate-vanadate
- bpV
bis(peroxo)oxovanadium(V)
- (bpV)2
bis(peroxo)oxovanadium(V) dimer
- bpVpic
bis(peroxo)picolinatooxovanadate(V)
-
13C
carbon-13
- EDTA
ethylenediaminetetraacetic acid
- EPR
electron paramagnetic resonance
- EXSY
exchange spectroscopy
-
1H
proton
- HSG
glutathione
- NAD
-nicotinamide adenine dinucleotide
- NADP
-nicotinamide adenine dinucleotide phosphate
- NADV
-nicotinamide adenine dinucleotide vanadate
- NMR
nuclear magnetic resonance (also referred to as magnetic resonance imaging)
- pVdipic
(dipicolinato)peroxovanadate(V)
- Vcit
(citrato)dioxovanadate(V)
- VEDTA
(ethylenediaminetetraacetato)dioxovanadate(V)
- Vmalto
bis(maltolato)-oxovanadium(IV)
- Voxal
bis(oxalato)dioxovanadate(V)
-
51V
vanadium-51
- V1
vanadate monomer
- V2
vanadate dimer
- V4
vanadate tetramer
- V5
vanadate pentamer
- UV-vis spectroscopy
ultraviolet-visible spectroscopy 相似文献
4.
H. Michibata J. Uchiyama Y. Seki T. Numakunai T. Uyama 《Biological trace element research》1992,34(3):219-223
It is a remarkable and previously unrecognized fact that ascidians, which are known to contain high levels of vanadium in
their blood cells, begin to accumulate vanadium during embryogenesis. This study revealed that the accumulation starts quite
dramatically 2 wk after fertilization, and 2 mo later, the amount of vanadium in larvae is 600,000 times higher than that
in the unfertilized egg. These results were obtained by neutron activation analysis, a highly sensitive method for determining
levels of vanadium, in theAscidia gemmata, the ascidian that contains the highest known levels of vanadium and accumulates vanadium at 150 mM in its blood cells, a concentration that corresponds to 4,000,000 times the concentration in sea-water. 相似文献
5.
Sugumaran M Robinson WE 《Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology》2012,163(1):1-25
Several species of ascidians (phylum Chordata, subphylum Urochordata) contain a group of oligopeptides called "tunichromes" in their blood cells. These peptides have been implicated in (a) metal chelation and accumulation/sequestration of vanadium or iron; (b) crosslinking of structural fibers in tunic formation, (c) wound healing and (d) defense reactions. However, their biosynthesis, metabolism, and biological function remain largely un-elucidated due to their extreme instability and high reactivity. Tunichromes and related compounds uniquely possess dehydrodopamine moieties, all originating from post-translational modification of peptidyl tyrosine. It is conceivable that the presence of such novel post-translationally modified groups provide attributes that are crucial for their biological roles. Therefore, we examined the chemistry and reactivity of tunichromes in light of the available knowledge of the biochemistry of simple monomeric dehydro-N-acyldopamine units. Based on the reactivity of such simple compounds, the potential biological activities of tunichromes are predicted. Their possible biosynthetic route from peptidyl tyrosine is critically evaluated to provide a better basis for unraveling their biological functions. Prevalence of dehydro-N-acyldopamine units in different tunichromes, some marine antibiotic compounds, insect cuticular sclerotizing precursors and some bioadhesive marine proteins may aid in the de novo design of unique biomaterials with potential antibiotic/adhesive properties. 相似文献
6.
A bovine kidney cell culture system was used to assess the relationship of vandium uptake and subcellular distribution to orthovanadate induced cytotoxicity. Twenty-four hr incubations with 20–1000 µM vanadium elicited 15–75% cytotoxicity. Concentration-related morphological changes from the control polygonal shape to the treated biopolar appearance were detected. Vanadium accumulated in cells via a multiphasic process; peak accumulation was achieved by 1 hr post-treatment and was followed by apparent decline, completed by 3 hr. A slower second phase of accumulation occured during the remainder of the 24 hr incubation period. A concentration-dependent accumulation resulted in a 50-fold increase in cellular vanadium content. Near maximum toxicity was achieved at a cellular vandium burden of approximately 5 nmoles/ 106 cells; further accumulation (up to 35 nmoles/ 106 cells) resulted in only a slight increase in the degree of toxicity. Subcellular distribution studies indicated 90% accumulation of vanadium in the soluble supernatant fraction (105,000xg supernatant) at varying stages of cytotoxicity. It was concluded that the multifaceted dependency of vanadium cytotoxicity on its cellular content may have resulted from a cellular balancing between proposed regulatory functions for vanadium and the interactions incurred with an excessive content.Abbreviations EDTA
ethylenediaminetetraacetic acid
- MDBK
Madin Darby bovine kidney
- MEM
minimum essential medium
- Na3VO4
sodium orthovanadate 相似文献
7.
A. S. Paschoa M. E. Wrenn N. P. Singh F. W. Bruenger S. C. Miller M. Cholewa K. W. Jones 《Biological trace element research》1987,13(1):275-282
Several geological formations of the Utah-Colorado mining region mined for uranium ore during and after World War II had been
mined earlier for vanadium. Therefore, most miners and millers from that region were exposed to those metals’ ores or tailings
at one time or another. Preliminary investigation to determine uranium and vanadium retained in the lungs of a former uranium
miner and miller from this region, who died of lung cancer (mesothelioma), showed a high nonuniform distribution of vanadium.
This observation led to the hypothesis that the vanadium content in the lungs could be associated with inhaled particles.
Further examination of spectra of characteristic X-rays obtained by scanning particle-induced X-ray emission (microPIXE) of
an autopsy sample of this lung indicated that vanadium was indeed present in localized sites within the 20-μm spatial resolution
of the proton beam. This work points out that the microPIXE-RBS (Rutherford backscattering) test for vanadium can be used
for site localization of inhaled particles retained in the lungs. Further studies are in progress to: (i) locate uranium-bearing
particles in lung tissues of former uranium miners and millers; and (ii) evaluate the local doses of alpha radiation received
from these particles. 相似文献
8.
Seale AP de Jesus LA Kim SY Choi YH Lim HB Hwang CS Kim YS 《Biotechnology letters》2005,27(4):221-225
The inhibition of protein-tyrosine phosphatase 1B (PTP1B) is a potential target for treatment of type 2 diabetes. Vanadium and zinc metal coordinated complexes have insulin-enhancing activities, and while vanadium compounds inhibit PTP1B, little is known on the mode of action of zinc compounds. In this study we developed an automated PTP1B inhibition assay that allows for a rapid assessment of the PTP1B inhibition strength of candidate compounds. Synthetic vanadium(IV) and zinc(II) complexes were evaluated: IC50 values for vanadium complexes ranged from 0.06 to 0.8m whereas for zinc compounds, values were above 10 m. Vanadium sulfate, a non-conjugated inorganic salt, had stronger inhibition activity than any of the conjugated metal complexes. Revisions requested 14 October 2004; Revisions received 6 December 2004 相似文献
9.
Alison Butler Helena S. Soedjak Meiram Polne-Fuller Aharon Gibor Catherine Boyen Bernard Kloareg 《Journal of phycology》1990,26(3):589-592
Aqueous Tri-SO4 buffer (pH 8.3) extracts of cortical and surface protoplasts of Macrocystis pyrifera (L.) C. Ag. Catalyzed the bromination of monochlorodimedone (2-chloro-5, 5-dimethyl-1, 3-dimedone, MCD) in the presence of hydrogen peroxide and bromide. The apparent bromo-peroxidase activity as measured by the bromination of MCD was inhibited by the presence of endogenous compounds which are probably polyphenolics compounds (i.e. polymers of phloroglucinol) or other inhibitors. The bromoperoxidase activity of the protoplast extracts increased substantially when the extracts were washed extensively with Tris-SO4 buffer (pH 8.3) by ultrafiltration. The bromoperoxidase activity of both surface and cortical protoplast extracts was dependent on the presence of vanadium, indicating that the bromoperoxidase present in cortical and surface cells of M. pyrifera is vanadium-bromoperoxidase. Halogenated compounds constitute one of the most significant classes of marine natural products. Since bromoperoxidases are assumed to be involved in the biosynthesis of these compounds, elucidation of the location of BrPO with in the algal tissue is important. 相似文献
10.
Yoshihara M Ueki T Yamaguchi N Kamino K Michibata H 《Biochimica et biophysica acta》2008,1780(2):256-263
The ascidians, the so-called sea squirts, accumulate high levels of vanadium, a transition metal. Since Henze first observed this physiologically unusual phenomenon about one hundred years ago, it has attracted interdisciplinary attention from chemists, physiologists, and biochemists. The maximum concentration of vanadium in ascidians can reach 350 mM, and most of the vanadium ions are stored in the +3 oxidation state in the vacuoles of vanadium-accumulating blood cells known as vanadocytes. Many proteins involved in the accumulation and reduction of vanadium in the vanadocytes, blood plasma, and digestive tract have been identified. However, the process by which vanadium is taken in prior to its accumulation in vanadocytes has not been elucidated. In the present study, a novel vanadium-binding protein, designated VBP-129, was identified from blood plasma of the vanadium-rich ascidian Ascidia sydneiensis samea. Although VBP-129 mRNA was transcribed in all A. sydneiensis samea tissues examined, the VBP-129 protein was exclusively localized in blood plasma and muscle cells of this ascidian. It bound not only to VO(2+) but also to Fe(3+), Co(2+), Cu(2+), and Zn(2+); on the other hand, a truncated form of VBP-129, designated VBP-88, bound only to Co(2+), Cu(2+) and Zn(2+). In a pull-down assay, an interaction between VanabinP and VBP-129 occurred both in the presence and the absence of VO(2+). These results suggest that VBP-129 and VanabinP function cooperatively as metallochaperones in blood plasma. 相似文献
11.
J.J.R. Fraústo da Silva 《Chemical Speciation and Bioavailability》2013,25(4):139-150
AbstractThe studies relative to the presence, state and combined form of vanadium in Amanita toadstools are reviewed critically. A possible role for the vanadium containing compound “amavadine” is suggested on the basis of the present knowledge of its structure, thermodynamic stability and oxidation-reduction behaviour. 相似文献
12.
Vanadium and diabetes 总被引:21,自引:0,他引:21
Poucheret Patrick Verma Subodh Grynpas Marc D. McNeill John H. 《Molecular and cellular biochemistry》1998,188(1-2):73-80
We demonstrated in 1985 that vanadium administered in the drinking water to streptozotocin (STZ) diabetic rats restored elevated blood glucose to normal. Subsequent studies have shown that vanadyl sulfate can lower elevated blood glucose, cholesterol and triglycerides in a variety of diabetic models including the STZ diabetic rat, the Zucker fatty rat and the Zucker diabetic fatty rat. Long-term studies of up to one year did not show toxicity in control or STZ rats administered vanadyl sulfate in doses that lowered elevated blood glucose. In the BB diabetic rat, a model of insulin-dependent diabetes, vanadyl sulfate lowered the insulin requirement by up to 75%. Vanadyl sulfate is effective orally when administered by either single dose or chronic doses. It is also effective by the intraperitoneal route. We have also been able to demonstrate marked long-terrn effects of vanadyl sulfate in diabetic animals following treatment and withdrawal of vanadyl sulfate. Because vanadyl sulfate is not well absorbed we have synthesized and tested a number of organic vanaditun compounds. One of these, bismaltolato-oxovanadiurn IV (BMOV), has shown promise as a therapeutic agent. BMOV is 2-3x more potent than vanadyl sulfate and has shown less toxicity. Recent studies from our laboratory have shown that the effects of vanadium are not due to a decrease in food intake and that while vanadium is deposited in bone it does not appear to affect bone strength or architecture. The mechanism of action of vanadium is currently under investigation. Several studies indicate that vanadiun is a phosphatase inhibitor and that vanadium can activate serine/threonine kineses distal to tbe insulin receptor presumably by preventing dephosphorylation due to inhibition of phosphatases Short-term clinical trials using inorganic vanadium compounds in diabetic patients have been promising. 相似文献
13.
Velusamy Arumugam Manigandan Venkatesan Saravanan Ramachandran Umamaheswari Sundaresan 《International journal of peptide research and therapeutics》2018,24(1):13-18
Marine ascidians are considered as one of the richest sources of bioactive compounds. The extraction and utilization of marine peptides have been attracted much attention owing to their potential health benefits. Most of the bioactive compounds from marine ascidians are already in different phases of the clinical and preclinical pipeline. They can be used in different functional and nutraceutical values due to their antineoplastic, antihypertensive, antioxidant, and antimicrobial properties. The screening in vivo and in vitro bioassays are coupled to the purification process for the exploration of its biological interest which is of great value. The growing significance to study marine natural products results from the discovery of novel pharmacological tools including potent anticancer drugs and other drugs are in clinical/pre-clinical trials. The present review highlights the recent research progress in marine ascidians’ peptides and its prospects for the future pharmaceutical development. 相似文献
14.
Yuebin Zhao Huixue Liu Qing Xia Xiaoda Yang Kui Wang 《Journal of inorganic biochemistry》2010,104(4):371-378
Vanadium compounds have been regarded as promising in therapeutic treatment of diabetes and in cancer prevention. In the present work, we studied the effects of vanadium compounds on mitochondria to investigate the mechanisms of toxicity. Mitochondria were isolated from rat liver and incubated with a variety of vanadium compounds, i.e. VOSO4, NaVO3, and vanadyl complexes with organic ligands. Our studies indicated that VO2+, , VO(acac)2 and VOcit (1-100 μM) could induce mitochondrial swelling in a concentration dependent manner and disrupt mitochondrial membrane potential (Δψm) in a time dependent manner, which is quite different from the rapid Δψm collapse caused by Ca2+ or CCCP (carbonyl cyanide m-chlorophenylhydrazone, a mitochondrial uncoupling reagent). Release of cytochrome c (Cyt c) was observed and could be inhibited by cyclosporin A (CsA), an inhibitor of the mitochondrial permeability transition pore (PTP). Interestingly, VOdipic caused release of Cyt c without mitochondrial swelling and Δψm disruption, an action previously only observed on the Bax protein, suggesting a potentially role of VOdipic in regulating PTP opening. In addition, all the vanadium compounds tested stimulated mitochondrial production of reactive oxygen species (ROS). Antioxidants, i.e. vitamin C and E, significantly delayed the Δψm disruption. Overall, our experimental evidence indicated vanadium compounds exhibited multiple actions on mitochondria. Vanadium compounds did induce oxidative stress on mitochondrial and thus caused PTP opening, which led to collapse of Δψm and Cyt c release as the initiation of cell apoptosis. 相似文献
15.
Maria João Pereira Eugénia Carvalho Debbie C. Crans 《Journal of inorganic biochemistry》2009,103(12):1687-1692
The effects of different vanadium compounds namely pyridine-2,6-dicarboxylatedioxovanadium(V) (V5-dipic), bis(maltolato) oxovanadium(IV) (BMOV) and amavadine, and oligovanadates namely metavanadate and decavanadate were analysed on basal and insulin stimulated glucose uptake in rat adipocytes. Decavanadate (50 μM), manifest a higher increases (6-fold) on glucose uptake compared with basal, followed by BMOV (1 mM) and metavanadate (1 mM) solutions (3-fold) whereas V5 dipic and amavadine had no effect. Decavanadate (100 μM) also shows the highest insulin like activity when compared with the others compounds studied. In the presence of insulin (10 nM), only decavanadate increases (50%) the glucose uptake when compared with insulin stimulated glucose uptake whereas BMOV and metavanadate, had no effect and V5 dipic and amavadine prevent the stimulation to about half of the basal value. Decavanadate is also able to reduce or eradicate the suppressor effect caused by dexamethasone on glucose uptake at the level of the adipocytes. Altogether, vanadium compounds and oligovanadates with several structures and coordination spheres reveal different effects on glucose uptake in rat primary adipocytes. 相似文献
16.
Susana B. Etcheverry Patricia A.M. Williams Viviana C. Sálice Daniel A. Barrio Evelina G. Ferrer Ana M. Cortizo 《Biometals》2002,15(1):37-49
A recently synthesized vanadyl(IV) complex with aspirin[VO(aspirin)ClH2O]2, has been thoroughly investigated by physicochemical techniques. In order to support the proposed structure, stoichiometry and the coordination sphere of the vanadium center, some studies such as elemental analysis, electronic (diffuse reflectance) and vibrational (infrared) spectroscopies, magnetic susceptibility, as well as the thermal behavior, were carried out. The bioactivity of the vanadium complex (VOAspi) was evaluated on two osteoblast-like cell lines in culture, being its cytotoxic effects stronger than the vanadyl cation as assessed by morphological changes and lipid peroxidation. These effects may be partially explained through the induction of the expression of Erks (Extracellular signal-regulated kinases) and the inhibition of the PTPases (Phosphotyrosine phosphatases) present in the cellular extracts. 相似文献
17.
Islam MK Tsuboya C Kusaka H Aizawa S Ueki T Michibata H Kanamori K 《Biochimica et biophysica acta》2007,1770(8):1212-1218
To better understand the mechanism of vanadium reduction in ascidians, we examined the reduction of vanadium(V) to vanadium(IV) by NADPH and the reduction of vanadium(IV) to vanadium(III) by L-cysteine methyl ester (CysME). UV-vis and electron paramagnetic resonance spectroscopic studies indicated that in the presence of several biologically relevant ligands vanadium(V) and vanadium(IV) were reduced by NADPH and CysME, respectively. Specifically, NADPH directly reduced vanadium(V) to vanadium(IV) with the assistance of ligands that have a formation constant with vanadium(IV) of greater than 7. Also, glycylhistidine and glycylaspartic acid were found to assist the reduction of vanadium(IV) to vanadium(III) by CysME. 相似文献
18.
M. A. Serra E. Sabbioni A. Marchesini A. Pintar M. Valoti 《Biological trace element research》1989,23(1):151-164
Vanadate ions are shown to inhibit horseradish, squash, and rat intestinal peroxidases by following the reaction spectrophotometrically
in a wide range of vanadate concentrations. I50 in phosphate buffer were 43, 9.4, and 535 μM, respectively. No inhibitory effect was found on cow milk lactoperoxidase and
beef liver catalase. Gel filtration of peroxidases in the presence of vanadate, as carried out by radioactive48V for horseradish peroxidases (either in aerobic or anoxic conditions) and neutron activation analysis (NAA) for squash peroxidase,
demonstrated a binding of vanadium to these enzymes in stoichiometric amounts. Electron paramagnetic resonance spectra of
the eluted peaks for the former peroxidase indicated that vanadium is in the +5 oxidation state, but an equilibrium between
V (V) and V (IV) in the assay conditions cannot be discarded. Although the inhibitory mechanism remains obscure, some hypotheses
are considered. The potential implications that the inhibitory effect of vanadium might have on plant and animal metabolism
are also discussed. 相似文献
19.
Ueki T Takemoto K Fayard B Salomé M Yamamoto A Kihara H Susini J Scippa S Uyama T Michibata H 《Zoological science》2002,19(1):27-35
Some ascidians (sea squirts) accumulate the transitional metal vanadium in their blood cells at concentrations of up to 350 mM, about 10(7) times its concentration found in seawater. There are approximately 10 different types of blood cell in ascidians. The identity of the true vanadium-containing blood cell (vanadocyte) is controversial and little is known about the subcellular distribution of vanadium. A scanning x-ray microscope installed at the ID21 beamline of the European Synchroton Radiation Facility to visualize vanadium in ascidian blood cells. Without fixation, freezing or staining realized the visualization of vanadium localized in living signet ring cells and vacuolated amoebocytes of two vanadium-rich ascidian species, Phallusia mammillata and Ascidia sydneiensis samea. A combination of transmission and fluorescence images of signet ring cells suggested that in both species the vacuoles contain vanadium. 相似文献
20.
Treberg JR Stacey JE Driedzic WR 《Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology》2012,161(4):323-330
Some suborders of ascidians (sea squirts) accumulate remarkable levels of the heavy metal vanadium while others accumulate negligible amounts. The function of this vanadium is unclear, but enhanced pentose phosphate pathway (PPP) has been implicated in its reduction and accumulation. We compared aspects of intermediary metabolism in coelomic cells from ascidian species that have a wide range of vanadium accumulation including non-accumulators. All species appear to have similar aerobic poise with no apparent link to vanadium accumulation. Similarly, all species examined have a limited anaerobic poise that does not seem to relate to vanadium levels. Based on the activities of phosphoglucose isomerase and glucose-6 phosphate dehydrogenase we demonstrate that, relative to the capacity for entry into glycolysis, vanadium-accumulating species have enhanced capacity to metabolize glucose-6 phosphate via the PPP compared to non-accumulators. This finding provides the first comparative support for enhanced PPP capacity linked to vanadium accumulation in tunicates. 相似文献