共查询到20条相似文献,搜索用时 31 毫秒
1.
Quorum sensing (QS) is a cell-to-cell signaling communication system that controls the virulence behavior of a broad spectrum of bacterial pathogens, participating also in the development of biofilms, responsible of the antibiotic ineffectiveness in many infections. Therefore, QS system is an attractive target for antimicrobial therapy. In this study, we compare the effect of seven structurally related coumarins against bacterial growth, biofilm formation and elastase activity of Pseudomonas aeruginosa. In addition, the anti-pathogenic capacity of the seven coumarins was evaluated on the wild type and the biosensor strain of Chromobacterium violaceum.The comparative study of coumarins showed that molecules with hydroxyl groups on the aromatic ring displayed higher activity on the inhibition of biofilm formation of P. aeruginosa over coumarins with substituents in positions 3 and 4 or without the double 3,4-bond. These 3 or 4-hydroxylated positions caused a decrease in the anti-biofilm activity obtained for coumarin. However, the hydroxyl group in position 3 of the pyrone ring was important for the inhibition of C. violaceum QS and elastolytic activity of P. aeruginosa. The effects observed were active independently of any effect on growth. According to our results, coumarin and its hydroxylated derivatives represent an interesting group of compounds to use as anti-virulence agents against the human pathogen P. aeruginosa. 相似文献
2.
E. O. Punina E. M. Machs E. E. Krapivskaya E. S. Kim E. V. Mordak Yu. A. Myakoshina A. V. Rodionov 《Russian Journal of Genetics》2012,48(7):684-697
The ITS1-5.8S rDNA-ITS2 regions of 33 accessions belonging to 16 species and five natural and garden interspecific hybrids of the genus Paeonia L. were sequenced. Chromatograms of the peony hybrids demonstrated the presence of the signals, corresponding to two different nucleotides at the positions differing in the parents, indicating that in the hybrids, no rDNA isogenization usually occurred, and they preserved rDNA of both parents. Analysis of these polymorphic sites (PS) showed that P. × majkoae was interspecific hybrid between P. tenuifolia and P. caucasica. The ITS of P. hybrida differs from ITS of P. × majkoae in 19 mutations. Because of this, P. × majkoae is definitely not synonymous to P. hybrida. Comparative analysis of ITS1-5.8S rDNA-ITS2 showed that species diversity in section Paeonia was based on recombination as a result of intraspecific hybridization of three haplotype families. Specifically, haplotypes A, typical of the P. tenuifolia and P. anomala genomes, haplotypes B, typical of P. mlokosewitschii and P. obovata, and haplotypes of family C, currently represented in rDNA of diploid and tetraploid forms of some Caucasian and Mediterranean species. The ITS regions many diploid peonies contain no dimorphic sites, while P. oreogeton, P. cambessedesii, P. rhodia, and P. daurica carry from 10 to 17 PS, and supposed to be the interspecific hybrids. Most of the tetraploid peonies contain from 6 to 18 PS in the ITS regions. These are alloploids with one of the parental genomes similar to that of P. mlokosewitschii (B1), or P. obovata (B3). The second parental genome in P. banatica, P. peregrina, and P. russii is represented by the genome, close to that of P. tenuifolia (A). P. macrophylla, P. mascula, P. coriacea, P. wittmanniana, and P. tomentosa carry genome of series B and genome of series C, which slightly resembles genome A. 相似文献
3.
Boscia angustifolia A. Rich. leaves were collected and subjected to a phytochemical investigation. Ten compounds; six flavonols, two coumarins, one methyl benzoate and one phthalate compound were isolated and identified for the first time from the studied taxa. Tamarixetin-3-O-β-sophoroside-7-O-methylether and rhamnocitrin-3-O-β-sophoroside are reported here as new natural compounds. The chemosystematic relationship between Boscia species is also discussed. 相似文献
4.
Aims
Rheum palmatum complex comprises all taxa within section Palmata in the genus Rheum, including R. officinale, R. palmatum, R. tanguticum, R. tanguticum var. liupanshanense and R. laciniatum. The identification of the taxa in section Palmata is based primarily on the degree of leaf blade dissection and the shape of the lobes; however, difficulties in species identification may arise from their significant variation. The aim of this study is to analyze the patterns of variation in leaf blade characteristics within and among populations through population-based sampling covering the entire distribution range of R. palmatum complex.Methods
Samples were taken from 2340 leaves from 780 individuals and 44 populations representing the four species, and the degree of leaf blade dissection and the shape of the lobe were measured to yield a set of quantitative data. Furthermore, those data were statistically analyzed.Important Findings
The statistical analysis showed that the degree of leaf blade dissection is continuous from lobed to parted, and the shape of the lobe is also continuous from broadly triangular to lanceolate both within and between populations. We suggested that taxa in section Palmata should be considered as one species. Based on the research on the R. palmatum complex, we considered that the quantitative characteristics were greatly influenced by the environment. Therefore, it is not reliable to delimitate the species according to the continuously quantitative vegetative characteristics. 相似文献5.
B. Jiang Q. F. Peng Z. G. Shen M. Möller E. X. Pi H. F. Lu 《Plant Systematics and Evolution》2010,290(1-4):1-20
Multivariate analysis of leaf shape, anatomy, and Fourier-transform infrared (FTIR) data of 27 Camellia species with secretory structures (sects. Archecamellia, Stereocarpus, Furfuracea, Chrysantha), together with three species from related genera, Gordonia and Tutcheria (Theacea), was conducted to clarify some taxonomic problems. Our results show that crystals occurring in adaxial epidermal cells are firstly observed in Chrysantha species, and the secretory structures described are in fact cork warts. Furthermore, we introduce a form coefficient (F c) to assess the shape of epidermal cells, since they are usually irregular and difficult to describe. Pearson correlation analysis indicates that F c is useful to assess epidermal cell shape. Principal component analysis (PCA) of leaf shape indicates that two species from section Archecamellia and two species from section Stereocarpus are significantly different from those in section Furfuracea. Cluster analysis of FTIR data visualizes the degree of affinity among the 30 species examined here, which is consistent with the cluster analysis (CA) of anatomical data, as illustrated in the dendrogram. Therefore, our study indicates that integrated leaf characters based on leaf shape, anatomy, and FTIR data are useful in the taxonomic treatment of Camellia species with secretory structures. Taxonomic controversies among the Camellia species with secretory structures could thus be successfully addressed using only a few intact or small portions of leaves. Moreover, our results tend to support that Chrysantha species should not be merged into section Archecamellia, and that section Heterogenea should not be recognized in taxonomic treatments of Camellia species with secretory structures. 相似文献
6.
Hiroki Tsukamoto Sueo Hisada Sansei Nishibe David G. Roux John P. Rourke 《Phytochemistry》1984,23(3):699-700
Esculetin and scopoletin were isolated from the bark of Olea africana while isoscopoletin and scoparone were isolated from the bark of Olea capensis. The distribution of these coumarins in Olea species from South Africa is described. 相似文献
7.
Xue Qin Wan Fan Zhang Yu Zhong Yun Hai Ding Chang Liang Wang Ting Xin Hu 《Plant Systematics and Evolution》2013,299(1):57-65
Unique historical factors and ecological conditions make Southwest China a natural distribution and variation center for trees of the genus Populus in China. However, little is currently known about the native poplars occurring in this region, and considerable doubt still exists regarding the classification and evolutionary relationships of poplar species. In this study, nuclear and chloroplast DNA (cpDNA) sequences were utilized to determine the genetic relationships and phylogeny of Populus species in Southwest China. The results suggest that P. pseudoglauca belongs to the section of Tacamahaca. Further, P. schneideri may be a natural hybrid of P. kangdingensis and P. cathayana and, thus, it should likely not be regarded as a variety of P. kangdingensis, as in the existing classification system. In addition, cluster analyses showed that P. gonggaensis may be derived from a cross between species of section Leucoides and P. cathayana or P. schneideri of section Tacamahaca, although it is still doubtful whether P. gonggaensis can be regarded as a separate species, due to its narrow distribution range. The parents of the Luding poplar may be P. yunnanensis and P. lancifolia. P. butuoensis showed a close affinity to species of section Leucoides and had a close relationship with P. gonggaensis or P. lasiocarpa. However, further research is needed in order to appropriately classify these as species or varieties. The incongruence between phylogenetic trees based on nuclear- and chloroplast-DNA sequence data may be due to the different inheritance patterns between nuclear- and cpDNA genome. 相似文献
8.
During a survey of fungal diversity associated with insects, mud, soil, and freshwater niches in different areas in Korea, nine interesting fungal strains were isolated. Based on their morphological characteristics and molecular phylogeny analyses, using a combined data set of β-tubulin (BenA), calmodulin (CaM), and second largest subunit of RNA polymerase (RPB2) sequences, the strains CNUFC AM-44, CNUFC JCW3-4, CNUFC S708, CNUFC WT202, CNUFC AS1-29, CNUFC JCW3-5, CNUFC JDP37, and CNUFC JDP62 were identified as Aspergillus alabamensis, A. floridensis, A. subversicolor, Penicillium flavigenum, P. laevigatum, P. lenticrescens, Talaromyces adpressus, and T. beijingensis, respectively. The strain CNUFC JT1301 belongs to series Westlingiorum in section Citrina and is phylogenetically related to P. manginii. However, slow growth when cultivated on CYA, MEA, CREA is observed and the property can be used to easily distinguish the new species from these species. Additionally, P. manginii is known to produce sclerotia, while CNUFC JT1301 strain does not. Herein, the new fungal species is proposed as P. aquadulcis sp. nov. Eight species, A. alabamensis, A. floridensis, A. subversicolor, P. flavigenum, P. laevigatum, P. lenticrescens, T. adpressus, and T. beijingensis, have not been previously reported in Korea. The present study expands the known distribution of fungal species belonging to the families Aspergillaceae and Trichocomaceae in Korea. 相似文献
9.
Eight linear furocoumarins and three coumarins were isolated and identified from Thamnosma texana. They were xanthotoxin, imperatorin, bergapten, alloimperatorin methyl ether epoxide, heraclenin, isopimpinellin, psoralen, oxypeucedanin, and the coumarins herniarin, osthol and thamnosmin. The linear furocoumarins appear to be agents that account for the known photosensitizing properties of Thamnosma texana, and consequently its colloquial name, ‘blisterweed.’ This is the first report on the occurrence of imperatorin, heraclenin, oxypeucedanin, herniarin or osthol in any Thamnosma species. 相似文献
10.
Recent fieldwork in the Late Eocene of Zambrana (Álava, Basque-Cantabrian Region) has yielded a new species of Pachynolophus: P. zambranensis nov. sp. According to the phylogenetic analysis made in this study, the Zambrana species is part of the monophyletic Pachynolophus, which is defined at least by three synapomorphies: significant progressive increase of the size in the M1/1-M3/3 series, being M3/3 outstandingly bigger than M2/2; moderate increase of the relative surface of M3/ with respect to M2/ surface, and weak labial cingula in the lower series. Pachynolophus includes the species P. duvali, P. livinierensis, P. garimondi, P. lavocati, P. zambranensis, P. boixedatensis and P. cesserasicus. P. molipotensis and P. cayluxi are not represented by sufficient material to attribute them to Pachynolophus. P. bretovensis does not share a synapomorphy of Pachynolophus and P. hookeri should not be referred to this genus. P. zambranensis shares several synapomorphies with P. garimondi and P. lavocati, which are: the posprotocrista of the P3-4/ series is a well-defined lophe; the P4/ has a subquadrangular outline and shows a strong posterolingual cingulum, forming a posterior widening; and there is an “incipient mesostyle” (pseudomesostyle?) in the upper molars. The species P. zambranensis is mainly characterized by lophodont dentition, with the postprotocrista obliquely oriented to the ectoloph in the P3-4/ series, and a “pseudomesostyle” also present in the P3-4/ series. It represents the first mention of the genus Pachynolophus described in the Late Eocene of the Iberian Peninsula. 相似文献
11.
The sesquiterpene lactones isolated from species in the genus Artemisia have been reviewed in an attempt to better understand the phylogeny and systematics of the four sections (subgenera), Abrotanum, Absinthium, Dracunculus and Seriphidium, proposed by Besser in 1829. The absence of hair on the receptacle is the only morphological characteristic separating species of Abrotanum from the species of Absinthium. There are no chemical characteristics segregating the species in these two subgenera since both produce eudesmanolides and guaianolides that are identical or biosynthetically similar. This suggests that the two subgenera could be combined into one (Artemisia) as proposed by Poljakov. The subgenus Seriphidium is composed of two geographical groups, one in the Old World and the other in the New World. The Old World species almost exclusively produce sesquiterpene lactones in the eudesmanolide class whereas the New World species (section Tridentatae) produce eudesmanolides and guaianolides, many of the latter being identical or structurally related to the sesquiterpene lactones in New World Abrotanum species. The chemical data in conjunction with geographic distributions suggest that the subgenus Seriphidium is polyphyletic and that the section Tridentatae originated from Abrotanum. Consequently, the Tridentate should be recognized as a subgenus separate and distinct from the Old World Seriphidium. There was insufficient information from the subgenus Dracunculus for interpretation. 相似文献
12.
M.G. Pimenov Yu.E. Sklyar A.A. Savina Yu.V. Baranova 《Biochemical Systematics and Ecology》1982,10(2):133-138
Ferula species of the section Palaeonarthex contains various sesquiterpenoids. While C-15 terpenoid coumarins of the iresan type are the most characteristic constituents of species in the subsection Foetidissimae, sesquiterpene lactones and C-10 terpenoid coumarns are found in the subsection Tumidae and esters of the sesquiterpene alcohols in the subsection Jaeschkaenae. The biogenetic relationships of the Ferula sesquiterpenoids and their chemosystematic value are discussed. 相似文献
13.
A multigene family that interacts with the amino terminus of plasmodium MSP-1 identified using the yeast two-hybrid system 总被引:1,自引:0,他引:1
下载免费PDF全文
![点击此处可从《Eukaryotic cell》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Merozoite surface protein 1 (MSP-1) is a high-molecular-weight protein expressed on the surface of the malaria merozoite in a noncovalent complex with other protein molecules. MSP-1 undergoes a series of proteolytic processing events, but no precise biological role for the various proteolytic fragments of MSP-1 or for the additional proteins present in the complex is known. Through the use of the yeast two-hybrid system, we have isolated genes encoding proteins that interact with a region of the amino-terminal proteolytic fragment of MSP-1 from the mouse parasite Plasmodium yoelii. This analysis has led to the isolation of two sequence-related molecules, one of which is the P. yoelii homologue of MSP-7 originally described in Plasmodium falciparum. BLAST analysis of the P. falciparum database has revealed that there are six related protein molecules present in this species encoded near each other on chromosome 13. In P. falciparum, we designated these molecules MSRP-1 to -5. Analysis of the P. yoelii database indicates a similar chromosomal organization for the two genes in the mouse parasite species. The three P. falciparum sequences with the highest degree of homology to the P. yoelii sequences isolated in the two-hybrid screen have been characterized at the molecular level (MSRP-1 to -3). Expression analysis indicated that the mRNAs are expressed at various levels in the different asexual stages. Immunofluorescence studies colocalized the expression of the MSRP molecules and the amino-terminal portion of MSP-1 to the surfaces of trophozoites. In vitro binding experiments confirmed the interaction between MSRP-1, MSRP-2, and the amino-terminal region of P. falciparum MSP-1. 相似文献
14.
Populus angustifolia, the narrowleaf cottonwood, is considered one of two native species of Populus section Tacamahaca restricted to western North America. Efforts to construct a definitive phylogeny of Populus spp. are complicated by ancient hybridization, but some phylogenetic analyses suggest P. angustifolia is more closely related to Asian congeners than to Populus trichocarpa, the other species of Populus section Tacamahaca in western North America. Because hosts and their obligate symbionts can display parallel phylogeographic patterns, we evaluated the possibility of a Beringian migration into North America by an Asian ancestor of P. angustifolia by determining the distributions, host preferences, and, in some cases, closest phylogenetic relatives of fungal leaf pathogens of P. angustifolia. Phyllactinia populi, a common foliar pathogen on Populus spp. in Asia but unknown on P. trichocarpa, was found on P. angustifolia in multiple sites. Mycosphaerella angustifoliorum, also unknown on P. trichocarpa, was commonly collected on P. angustifolia. Conversely, many common foliar pathogens of P. trichocarpa in western North America were not found on P. angustifolia; only Melampsora×columbiana and Drepanopeziza populi were common to both Populus species. Phylogenetic analyses revealed that M. angustifoliorum was not part of the diversification of Mycosphaerella on Populus that includes all other Mycosphaerella species on Populus in North America: Mycosphaerella populicola, Mycosphaerella populorum, M. sp. 1, and M. sp. 2. The latter two undescribed species represent a newly discovered diversification of M. populorum in western North America. Overall, the leaf pathogen community of P. angustifolia is consistent with a Beringian migration into North America by the ancestor of P. angustifolia. 相似文献
15.
Many plants are adapted to an eroded landscape with a large proportion of virgin soil. Open and disturbed soils are today almost only restricted to agricultural fields with high loads of fertilizers. We conducted a pot experiment in order to investigate growth and nutritional constraints of one calcicole species, Anisantha (syn. Bromus) tectorum, and one calcifuge species, Rumex acetosella, in decalcified topsoil and recently exposed calcareous subsoil from a field experiment in sandy grassland. In the pot experiment we implemented one treatment where we limed topsoil with CaCO3 to the same amount as in subsoil.The subsoil had approximately 10% CaCO3 and both species grew less in this soil compared to the topsoil, which had less than 1% CaCO3. Germination rate of A. tectorum was higher in subsoil than in topsoil or limed topsoil. P fertilization of the limed topsoil counteracted the negative liming effect for A. tectorum, but only partly so for R. acetosella. P fertilization of subsoil increased the shoot biomass of A. tectorum, but not of R. acetosella. P concentration in plants was not reduced when growing on subsoil or limed topsoil compared to topsoil. The results show that lime addition may reduce the P availability also to calcicole species such as A. tectorum and we found indications for that Ca toxicity may be a causing factor for the calcifuge behavior of R. acetosella. The significance of the results for conservation management practices in sandy grasslands is discussed. 相似文献
16.
Nadezhda L. Bolsheva Alexander V. Zelenin Inna V. Nosova Alexandra V. Amosova Tatiana E. Samatadze Olga Yu. Yurkevich Nataliya V. Melnikova Daria A. Zelenina Alexander A. Volkov Olga V. Muravenko 《PloS one》2015,10(4)
The wide variation in chromosome number found in species of the genus Linum (2n = 16, 18, 20, 26, 28, 30, 32, 36, 42, 72, 84) indicates that chromosomal mutations have played an important role in the speciation of this taxon. To contribute to a better understanding of the genetic diversity and species relationships in this genus, comparative studies of karyotypes and genomes of species within section Syllinum Griseb. (2n = 26, 28) were carried out. Elongated with 9-aminoacridine chromosomes of 10 species of section Syllinum were investigated by C- and DAPI/С-banding, CMA and Ag-NOR-staining, FISH with probes of rDNA and of telomere repeats. RAPD analysis was also performed. All the chromosome pairs in karyotypes of the studied species were identified. Chromosome DAPI/C-banding patterns of 28-chromosomal species were highly similar. Two of the species differed from the others in chromosomal location of rDNA sites. B chromosomes were revealed in all the 28-chromosomal species. Chromosomes of Linum nodiflorum L. (2n = 26) and the 28-chromosomal species were similar in DAPI/C-banding pattern and localization of several rDNA sites, but they differed in chromosomal size and number. The karyotype of L. nodiflorum was characterized by an intercalary site of telomere repeat, one additional 26S rDNA site and also by the absence of B chromosomes. Structural similarities between different chromosome pairs in karyotypes of the studied species were found indicating their tetraploid origin. RAPD analysis did not distinguish the species except L. nodiflorum. The species of section Syllinum probably originated from a common tetraploid ancestor. The 28-chromosomal species were closely related, but L. nodiflorum diverged significantly from the rest of the species probably due to chromosomal rearrangements occurring during evolution. 相似文献
17.
The genus Cousinia Cass. (Family Asteraceae) with about 600–700 species is distributed in central and western Asia. Iran has more than 200 Cousinia species that grow in mountainous areas. Cousinia is not monophyletic and with the genus Arctium, comprise the Arctium-Cousinia complex. The Cousinia species are distributed in 70 sections. The species relationship in the sect. Cousinia is not known, moreover, in few cases, inter-specific hybrids and intermediate forms are recognized. Therefore, we undertook a detailed morphological, micro-morphological and molecular (Cp-DNA and ISSRs) study of the section Cousinia with the following objectives: 1- to delimitate the species, 2- to identify potential intermediate forms, 3- to investigate the species inter-relationship. The present study revealed that combination of morphological, micro-morphological and ISSR data can delimit the species. We did not encounter any intermediate forms throughout the studied area. AMOVA and STRUCTURE analysis revealed that the species of this section are genetically differentiated but have some degree of shared common alleles. Reticulation analysis by T-REX also showed some degree of gene flow for Cp-DNA. This low degree of genetic and morphological variability in Cousinia, can help the species to cope with environmental variability they face. 相似文献
18.
Two new coumarins have been isolated from the aerial parts of Amyris balsamifera. On the basis of spectral and chemical data, these have been identified as (R)-(+)-6-(2′-hydroxy-3′-methyl-3′-butenyl)-7-methoxycoumarin and balsamiferone, 7-hydroxy-3,6-bis (3′-methyl-2′-butenyl)-coumarin. 相似文献
19.
Sequence data from a broad geographical region and different habitats show that the Inocybe praetervisa clade is comprised of four closely related species. These species of section Marginatae are characterized by having nodulose spores and a stipe that is abundantly pruinose only in the upper half. Inocybe praetervisa occurs in Southern Europe in mountainous mixed coniferous forests, and is not confirmed from Northern Europe. Inocybe rivularis occurs in northern boreal forests up to the lower alpine zone, associated with Betula in moist habitats, and is not confirmed from Southern Europe. Inocybe taxocystis is confirmed as a later synonym of I. favrei. The species has a wide geographical distribution range in Europe, mainly restricted to the alpine zone and moist soils, associated with Salix herbacea. Inocybe arctica is here described as a new species. It occurs in the arctic and higher alpine zones, associated with Dryas octopetala, Salix polaris, S. reticulata and S. herbacea. All species except I. arctica are shown to have an intercontinental distribution range and are confirmed from North America. Sequence data suggest the occurrence of one additional species in the alpine zone of China. A key to the species in the I. praetervisa group is provided. 相似文献
20.
《Biochemical Systematics and Ecology》2005,33(7):651-658
GC–MS analyses of dichloromethane extracts of the mandibular glands from three obligate symbiotic Psuedomyrmex ant species of Acacia collinsii from Costa Rica: Pseudomyrmex flavicornis (synonym Pseudomyrmex belti), Psuedomyrmex spinicola, and Psuedomyrmex nigrocincta, showed distinct differences in the 16 ketones, 15 alcohols, 2 aldehydes and 2 carboxylic acids that were identified. Different compounds were the major component from each species: P. flavicornis, 3-octanone; P. spinicola, 4-methyl-3-heptanone; and P. nigrocincta, 3-methyl-2-hexanol. The secretion of P. flavicornis contained 10 compounds not found in the other species, including the two terpene alcohols, citronellol and geraniol. The secretions of P. spinicola and P. nigrocincta had 12 compounds in common, that were not found in P. flavicornis' secretion. The similarity of the mandibular gland secretion of P. spinicola and P. nigrocincta may indicate that they are more closely related to each other than either is to P. flavicornis. The components from the mandibular gland of Crematogaster rochai, another ant associated with this acacia, are 2- and 3-methylbutanoic acid, 3-octanone, 3-octanol, 6-methyl-3-octanol and 3-nonanone. 相似文献