ACTL6a Enforces the Epidermal Progenitor State by Suppressing SWI/SNF-Dependent Induction of KLF4

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Highlights? ACTL6a is downregulated during epidermal differentiation ? ACTL6a loss in epidermis causes progenitor loss and terminal differentiation ? ACTL6a is required for repression of KLF4 and other epidermal differentiation genes ? ACTL6a prevents binding of the SWI/SNF complex to differentiation genes     

CED-1, CED-7, and TTR-52 regulate surface phosphatidylserine expression on apoptotic and phagocytic cells

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Highlights? PS is expressed on the surface of apoptotic and phagocytic cells during apoptosis ? CED-7 and TTR-52 mediate time-dependent loss of PS from surface of apoptotic cells ? CED-7 and TTR-52 promote generation of extracellular PS vesicles ? CED-7/TTR-52/CED-1 promote phagocyte PS expression, important for corpse engulfment     

β1 Integrin Establishes Endothelial Cell Polarity and Arteriolar Lumen Formation via a Par3-Dependent Mechanism

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Highlights? β1 integrin deletion results in loss of endothelial polarity and vessel patency ? β1 integrin regulates cell-cell adhesion protein distribution in the endothelium ? Polarity loss is typified by decreased Par3 expression ? Par3 can partially rescue lumen formation in the β1 integrin mutant     

Myosin-V Opposes Microtubule-Based Cargo Transport and Drives Directional Motility on Cortical Actin

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Highlights? Myosin-V alternates between active and passive actin-binding modes ? Myosin-V opposes kinesin-propelled cargo redistribution ? Myosin-V anchors kinesin-propelled cargo in actin-rich areas ? Myosin-V can drive medium-range directional transport at the cell periphery     

AMPK Is a Negative Regulator of the Warburg Effect and Suppresses Tumor Growth In Vivo

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Highlights? Loss of AMPKα1 cooperates with the Myc oncogene to accelerate lymphomagenesis ? AMPKα dysfunction enhances aerobic glycolysis (Warburg effect) ? Inhibiting HIF-1α reverses the metabolic effects of AMPKα loss ? HIF-1α mediates the growth advantage of tumors with reduced AMPK signaling     

Animal production for efficient phosphate utilization: from optimized feed to high efficiency livestock

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Highlights? Group feeding animals according to their physiological state will reduce P consumption. ? Reducing P in feed and precision feeding of P will increase efficiency of P utilization by livestock. ? Addition of phytase enzyme will increase P availability especially in swine and poultry. ? Transgenic plants and animals offer a way for efficient phosphate uptake. ? Nutritional genomics is expected to improve efficiency of P utilization in livestock.     

Structural and functional analysis of HtrA1 and its subdomains

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Highlights? IGFBP-like domain structure suggests that it is incompetent for IGF binding ? Kazal-like domain structure shows distorted “P1” loop incompetent for inhibition ? Protease domain shows catalytic readiness in apo form ? SAXS envelope for full-length HtrA1     

Loss of Rb Cooperates with Ras to Drive Oncogenic Growth in Mammalian Cells

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Highlights? Oncogenic Ras drives weak growth through the PI3K canonical growth pathway ? A novel E2F-independent role for the Rb family in cell biogenesis ? Ras and loss of Rb synergize to give robust and sustained cell growth ? PI3K pathway is crucial for sustained growth, acting to maintain nutrient uptake     

Biologically and chemically mediated adsorption and precipitation of phosphorus from wastewater

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Highlights? Phosphorus (P) ineffluent can be removed by biological/chemical-driven adsorption and precipitation. ? Phosphorus adsorption by filters and struvite formation are promising options in this field. ? Emerging adsorption approaches include P removal by polymers/nanomaterials.     

Cdc42 activity regulates hematopoietic stem cell aging and rejuvenation

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Highlights? Constitutively increasing Cdc42 activity causes aging of young HSCs ? Elevated Cdc42 activity correlates with a loss of cell polarity in aged HSCs ? Inhibition of Cdc42 activity rejuvenates aged LT-HSC function ? Cdc42 inhibition restores the level and spatial distribution of AcH4K16     

Stalled fork rescue via dormant replication origins in unchallenged S phase promotes proper chromosome segregation and tumor suppression

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Highlights? An unstable MCM2-7 complex results in a loss of dormant origins in Mcm4^Chaos3 cells ? A loss of dormant origins impairs stalled fork recovery in unchallenged S phase ? A loss of dormant origins increases replication intermediates in prophase ? Replication intermediates in M phase are a likely cause of chromosome instability     

Microtubules Enable the Planar Cell Polarity of Airway Cilia

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Highlights? PCP proteins are asymmetric at apical junctions in the airways before ciliogenesis ? PCP protein asymmetry and cilium orientation emerge gradually during development ? Cilium misorientation correlates with loss of PCP protein asymmetry in PCP mutants ? Microtubules appear to organize cilium orientation and PCP protein asymmetry     

Phosphorus: a limiting nutrient for humanity?

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Highlights? P is essential to build key molecules, such as RNA needed for rapid growth. ? P is often limiting to productivity in ecosystems, including to crops. ? Large amounts of P are mined annually to make fertilizer to produce food. ? Current P use is unsustainable owing to ecosystem impacts and uncertainties about fertilizer production. ? A food system redesign to combine P-efficient crops with P recycling is needed.     

Calorie Restriction and SIRT3 Trigger Global Reprogramming of the Mitochondrial Protein Acetylome

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Highlights? MS quantifies 1,578 mitochondrial acetyl sites altered during CR and loss of SIRT3 ? SIRT3 functions as a prominent regulator in CR adaptation ? CR and SIRT3 regulate previously unrecognized processes in mitochondria ? We provide an acetylation atlas for understanding mitochondrial regulation in CR     

GRSF1 Regulates RNA Processing in Mitochondrial RNA Granules

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Highlights? GRSF1 resides in the mitochondrial matrix and is required for mitochondrial function ? GRSF1 is required for the processing of tRNA-containing and tRNA-lacking precursors ? GRSF1, RNase P, and nascent RNA are part of “mitochondrial RNA granules” ? Mitochondrial RNA granules are functionally linked to RNA processing     

Phosphorus recovery from wastewater through microbial processes

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Highlights? EBPR is a mature, effective technology to concentrate P from dilute streams. ? EBPR sludge is rich in phosphorus: it can be applied to land. ? Phosphorus in EBPR sludge can be recovered as struvite; P needs to first be solubilized. ? Solubilization of P through anaerobic digestion is attractive, but complexing with metal ions needs to be minimized.     

FGF9 and FGF20 maintain the stemness of nephron progenitors in mice and man

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Highlights? FGF20 and FGF9 are the niche ligands for developing metanephric kidney progenitors ? FGF9 is expressed in duct epithelia; both ligands are expressed by progenitor cells ? FGF20 loss in humans, or FGF9/20 loss in mice, results in kidney agenesis ? FGF9/20 can maintain competence of sorted, pure progenitor cell populations in vitro     

Thrombopoietin-Increased DNA-PK-Dependent DNA Repair Limits Hematopoietic Stem and Progenitor Cell Mutagenesis in Response to DNA Damage

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Highlights? Mpl loss increases γ-irradiation-induced genomic instability in HSPCs ? TPO promotes DNA repair in vitro and in vivo in HSPCs ? TPO increases DNA-PK activity and NHEJ-mediated repair efficiency in HSPCs ? A single TPO injection before mouse TBI limits long-term HSC injury and mutagenesis     

Phage dUTPases Control Transfer of Virulence Genes by a Proto-Oncogenic G Protein-like Mechanism

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Highlights? dUTPases are signaling molecules acting in analogous manner to the G proteins ? Binding to dUTP induces a conformational change in the phage-encoded dUTPases ? The dUTP-bound form of the phage dUTPases induces transfer of virulence genes ? The dUTPase P loop-like motif V is the molecular switch of the signaling mechanism     

Structure of Phosphorylated SF1 Bound to U2AF65 in an Essential Splicing Factor Complex

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Highlights? Splicing factor SF1 phosphorylation on a conserved SPSP motif is required in vivo ? SPSP phosphorylation (P) induces local folding within an SF1/U2AF⁶⁵ interface ? Phosphorylation promotes an acutely bent (P)SF1/U2AF⁶⁵/RNA conformation