共查询到20条相似文献,搜索用时 31 毫秒
1.
Zyakun A. M. Kosheleva I. A. Zakharchenko V. N. Kudryavtseva A. I. Peshenko V. A. Filonov A. E. Boronin A. M. 《Microbiology》2003,72(5):592-596
The study deals with a comparative analysis of the relative abundances of the carbon isotopes 12C and 13C in the metabolites and biomass of the Burkholderia sp. BS3702 and Pseudomonas putida BS202-p strains capable of utilizing aliphatic (n-hexadecane) and aromatic (naphthalene) hydrocarbons as sources of carbon and energy. The isotope compositions of the carbon dioxide, biomass, and exometabolites produced during the growth of Burkholderia sp. BS3702 on n-hexadecane (13C = –44.6 ± 0.2) were characterized by the values of 13CCO
2 = –50.2 ± 0.4, 13Cbiom = –46.6 ± 0.4, and 13Cexo = –41.5 ± 0.4, respectively. The isotope compositions of the carbon dioxide, biomass, and exometabolites produced during the growth of the same bacterial strain on naphthalene (13C = –21 ± 0.4) were characterized by the isotope effects 13CCO
2 = –24.1 ± 0.4, 13Cbiom = –19.2 ± 0.4, and 13Cexo = –19.1 ± 0.4, respectively. The possibility of using the isotope composition of metabolic carbon dioxide for the rapid monitoring of the microbial degradation of petroleum hydrocarbons in the environment is discussed. 相似文献
2.
Recently, we have reported purification and characterization of a de-N-glycosylating enzyme, peptide:N-glycanase (PNGase) found in C3H mouse fibroblast L-929 cells, and designated L-929 PNGase [Suzuki T, Seko A, Kitajima K, Inoue Y, Inoue S (1994)J Biol Chem
269, 17611–18]. The unique properties of L-929 PNGase are that the enzyme had a high affinity to the substrate glycopeptide (e.g.K
m=114 µm for fetuin derived glycopentapeptide) and that the PNGase-catalysed reaction is strongly inhibited by the released free oligosaccharides but not by the free peptides formed, suggesting that L-929 PNGase is able to bind to a certain type of carbohydrate chain. In this study, we report the new findings of the mannan-binding property of L-929 PNGase; the de-N-glycosylating enzyme activity of L-929 PNGase was inhibited by yeast mannan and triomannose, Man1 3(Man1 6)Man, but not by mannose and -methyl-d-mannoside. Furthermore, L-929 PNGase was revealed to bind to the glycan moiety of yeast mannan by using mannan-conjugated Sepharose 4B gel as a ligand, suggesting that L-929 PNGase could serve not only as an enzyme but also as a carbohydrate recognition proteinin vivo. Such dual properties found for animal-derived L-929 PNGase are unique and are not shared with other previously characterized plant- and bacterial-origin PNGases — PNGase A and PNGase F, respectively.Abbreviations GLC
gas liquid chromatography
- GlcNAc-Asn
2-acetamido-1--(l-aspartamido)-1,2-dideoxy-d-glucose
- BSA
bovine serum albumin
- DTT
dithiothreitol
- EDTA
ethylenediaminetetraacetic acid
- Gal
d-galactose
- GlcNAc
N-acetyl-d-glucosamine
- Man
d-mannose; triomannose, Man1 3(Man1 6)Man;
- MES
2-(N-morphorino)ethanesulfonic acid
- NeuAc
N-acetyl-neuraminic acid
- PNGase
peptide:N
4-(N-accetyl-glucosaminyl)asparagine amidase (peptide:N-glycanase,EC 3.5.1.52)
- PNP
p-nitrophenyl 相似文献
3.
Jeannine Horowitz 《Molecular & general genetics : MGG》1985,198(3):482-485
Summary The molecular basis of a lectin gene mutation in the Phaseolus vulgaris cultivar Pinto was investigated. Rocket immunoelectrophoresis studies showed that seed lectin is reduced approximately 40-fold in comparison to the normal Phaseolus vulgaris cultivar Contender. DNA gel blot studies using a lectin cDNA probe suggested that the Pinto and Contender varieties contain similar numbers of lectin genes, although qualitative differences were observed in the gel blot banding patterns. Hybridization of the lectin cDNA probe to gel blots containing normal and mutant embryo mRNAs produced a 1 kb mRNA band in both mRNA populations. However, the amount of lectin mRNA was reduced approximately tenfold in the mutant Pinto cultivar. Together, these findings suggest that the reduced seed lectin level is due, in part, to a reduction in seed lectin mRNA.Abbreviations mRNA
messenger RNA
- cDNA
complementary DNA
- SDS-PAGE
sodium dodecyl sulfate polyacrylamide gel electrophoresis
- BSA
bovine serum albumin
- kd
kilodaltons
- kb
kilobases 相似文献
4.
M. Kawase 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1994,89(7-8):861-864
The restriction landmark genomic scanning (RLGS) method was applied to rice, using two Japanese cultivars, Nipponbare and Koshihikari, and a Chinese landrace, LiuZhou'Bao'Ya'Zao'. More than 3000 landmarks were detected as spots on the individual autoradiograms of each cultivar. Nipponbare and LiuZhou' Bao'Ya'Zao' showed apparently different RLGS profiles, from which the genetic similarity (GS) between them was estimated as 0.344. Although the two Japanese cultivars, Nipponbare and Koshihikari showed quite similar RLGS profiles, they were easily distinguished on the basis of the presence or absence of specific spots; the GS value between them was calculated as 0.980. The RLGS method is shown to be a powerful fingerprinting technique, especially for the classification and identification of cultivars in rice and probably in other crops as well. 相似文献
5.
Beta-adrenergic control of blood oxygen affinity in acutely hypoxia exposed rainbow trout 总被引:1,自引:1,他引:0
Tetens Vilhelm Christensen Niels Juel 《Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology》1987,157(5):667-675
Summary Acute exposure of rainbow trout to hypoxic water (
=40 mmHg, 15 °C) caused a significant (P<0.01) increase in blood O2 affinity, from the normoxicP
50 value (at pHe 7.93) of 23.2±1.1 mmHg to about 19 mmHg, within 5 min. Specimens injected with the -antagonist propranolol showed no change in bloodP
50, despite a more pronounced reduction of arterial
during the hypoxic exposure.The change in bloodP
50 coincided with an increase in plasma catecholamines, notably noradrenaline. There was no change in the molar ratios of ATPHb4 and GTPHb4. The altered bloodP
50, however, correlated with an alkalinization and an increased sodium concentration of the red cells. This red cell alkalinization can be explained by -adrenergic stimulation of a membrane bound Na+/H+ antiporter.Propranolol injection into normoxic resting trout caused a significant decrease in
and increase in
indicating -adrenergic control of gas exchange in the gills. 相似文献
6.
L. E. Francois 《Plant and Soil》1992,147(2):163-170
The boron tolerance of two summer squash cultivars (Cucurbita pepo L. Aristocrat Zucchini and Peter Pan Scallop) and one winter squash cultivar (Cucurbita moschata Poir. Butter boy) was determined in large, outdoor sand cultures. Boron treatments were imposed by irrigation with culture solutions that contained 1.0, 3.0, 6.0, 9.0, 12.0, or 15.0 mg B L-1. Relative fruit yields of Zucchini, Scallop, and Butter boy were reduced 5.2%, 9.8%, and 4.3% with each unit (mg L-1) increase in soil solution B (Bsw)>2.7, 4.9, and 1.0 mg B L-1, respectively. Reduced yields of all cultivars were attributed to a reduction in fruit number and not fruit size. Boron concentrations in leaves and fruit were directly correlated to Bsw. 相似文献
7.
The presence of algae within the cases ofAgapetus fuscipes was investigated. Cases recognised as dirty or clean with the naked eye had more and less algal growth, respectively. Larvae in the former survived significantly longer when starved in the laboratory. It is suggested that the presence of algae within the cases would be of ecological advantage during periods of flood. 相似文献
8.
Stefan Schillberg Martin Zimmermann Carl-Christian Emeis 《Applied microbiology and biotechnology》1991,35(2):242-246
Summary Rare-mating of closely related Saccharomyces cerevisiae and S. diastaticus strains led to the formation of different hybrids. Mating-type switching and chromosome losses could be observed by means of classical genetic analysis and pulsed field gel electrophoresis of intact chromosomes. The latter was facilitated by extensive chromosome length polymorphism in both strains. When crossing the two haploid strains S. cerevisiae 41 and S. diastaticus ATCC 28339 , two different types of hybrids occurred. Both types showed complete addition of both parental genomes, one a-status and the other -status. The -status could be explained by assuming a transient premutational lesion in MAT . Usually lesions are repaired after a mating event and the -mating type is restored. When crossing a diploid S. diastaticus strain, isogenic to the one previously mentioned, with the haploid S. cerevisiae strain, three different types of hybrids could be distinguished regarding their mating-types. It was possible to prove that the haploid S. diastaticus strain ATCC 28339 is disomic and the diploid hybrid, named 41ATCC-b, is trisomic for chromosome I. This could be shown by means of electrophoretic karyotyping of the hybrid and of the four single-spore cultures from one ascus of the hybrid. 相似文献
9.
Mammalian brain tubulin is an heterodimer; both and exist in 6–7 isotypic forms which differ in their amino acid sequences. By the use of isotype-specific monoclonal antibodies, we have previously shown that we can purify the
II,
III, anda
IV tubulin dimers from bovine brain. We have also observed that these isotypes differ in their distributionin vivo and their polymerization and drug-binding propertiesin vitro. We have now explored the question of whether the isotypically purified dimers differ in their overall conformation using as probes compounds of theN,N-polymethylenebis (iodoacetamide) series which are known to form discrete intrachain cross-links in-tubulin. These compounds have the structure ICH2CONH(CH2)
n
NHCOCH2I. One of these cross-links, designated
s, is between cys12 and either cys201 or cys211. The other, designated
*, is between cys239 and cys354. The
* cross-link forms in
II and
IV but not in
III; this is not surprising in view of the fact that
III has serine at position 239 instead of cysteine. However,
III is also unable to form the
s cross-link, although it appears to have all three cysteines which may be involved in the cross-link. This suggests that at least one of the sulfhydryls involved in the cross-link may be inaccessible in
III. Although both
II and
IV can form the
s cross-link, the dependence on cross-linker chain length is different.
II forms
s with derivatives in whichn=2, 4, 5, 6, and 7 but not with those in whichn=3 or 10. In contrast,
IV forms
s with derivatives in whichn=2, 3, 4, 5, 6, 7, and 10. These results imply that the
s sulfhydryls are slightly more accessible in
IV and are therefore less dependent on the conformation of the cross-linker to react with it. It appears, therefore, that the
II,
III, and
IV dimers each have unique conformations. This may help to explain the different assembly and drug-binding properties of these dimers. 相似文献
10.
M. K. Banerjee M. K. Kalloo 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1987,73(5):707-710
Summary One hundred and twenty-two varieties, lines and wild accessions of Lycopersicon were screened under three different regimes during the autumn/winter season of 1982–83 and 1983–84 for resistance to tomato leaf curl virus (TLCV). L. hirsutum f. glabratum (B6013) and L. hirsutum f. typicum (A1904) proved to be highly resistant to TLCV in all three environments. Various accessions of L. peruvianum were also highly resistant. L. pimpinellifolium (A1921) exhibited no TLCV symptoms within 90 days. Of the cultivated varieties, Acc 99 exhibited the minimim score for susceptibility; AC 142, Collection No. 2, Kalyanpur Angurlata and HS 101 had a low rating for virus incidence. The inheritance of resistance was studied in the interspecific crosses between a TLCV resistant line of L. pimpinellifolium (A1921) and five (HS 101, HS 102, HS 110, Pusa Ruby and Punjab Chhuhara) susceptible cultivars of L. esculentum. Parents, F1, F2 and backcross progenies were artificially inoculated with local strains of TLCV using vector the viruliferious whitefly, Bemisia tabaci (Genn.). Data indicated that the resistance of L. pimpinellifolium (A 1921) is monogenic and incompletely dominant over susceptibility. 相似文献
11.
Nichola J. K. Hole Edmundo Lamoyi Masanori Komatsu Nagaradona Harindranath Glendowlyn O. Young-Cooper Rose G. Mage 《Immunogenetics》1988,28(2):99-107
In order to investigate linkage, we used serum allotypes of the two rabbit C
isotypes and restriction fragment length polymorphisms (RFLPs) of the genes for V
, C
, and T-cell receptor C
. The inheritance of these genetic markers was studied through backcross and F2 matings. Southern analysis and hybridization of genomic DNA with a C probe detected a 5 kb Pst I fragment linked to expression of the K2bas1 allotype and the presence of the 1b
bas gene and a 6.6 kb Pst I fragment linked to the expression of the K1b9 allotype, the presence of the 2
bas2 gene and lack of expression of the K2bas1 allotype. A V probe detected a 1.3 kb Eco RI fragment linked to the presence of the 1b
bas gene and expression of the K2bas1 allotype. In contrast, the 9 or 14 kb Eco RI RFLP (C
a or C
b) detected with a Tcr
chain probe segregated independently from C allotypes and RFLPs. It has previously been found that C and C
are also unlinked in man, whereas in the mouse they are linked at a distance of 8 centimorgans. 相似文献
12.
A. Kanamori K. Kitajima Y. Inoue S. Inoue Z. Xulei C. Zuber J. Roth J. Ye F. A. Troy II 《Histochemistry and cell biology》1994,101(5):333-340
Two particular types of sialoglycoproteins have been detected in fish: polysialoglycoproteins containing 28-linked polysialic acid (8Neu5Gc2)
n
present in unfertilized Salmonidae fish eggs, and glycoproteins bearing oligo/polymers of deaminated neuraminic acids (KDN) found in the vitelline envelope of the eggs and ovarian fluid. We report the preparation and characterization of a monoclonal antibody specifically recognizing oligo/polymers of KDN sequences in glycoproteins and its application in immunohistochemistry. Fusion of spleen cells from a BALB/c mouse immunized with a KDN-rich glycoprotein (KDN-gp) containing (8KDN2)
n
6(KDN23Gal13GlNAc13) GalNAc1 residues, with mouse myeloma cells yielded a hybrid cell line producing a monoclonal antibody that bound to KDN-gp, but not to KDN-gp depleted of KDN residues. The specificity of the monoclonal antibody, designated mAb.kdn8kdn, was determined by an enzyme-linked immunosorbent assay using KDN-gp samples that varied in KDN content. These antigens were prepared by the selective removal of KDN residues from the native KDN-gp. The mAb.kdn8kdn reacted most strongly with the intact KDN-gp and less strongly with KDN-gp samples containing decreased numbers of KDN residues. The mAb.kdn8kdn was shown specifically to recognize the 28-linked oligo/polyKDN sequences, (8KDN2)
n
, and to be able to distinguish specifically (8KDN2)
n
chains from (8Neu5Ac2)
n
and (8Neu5Gc2)
n
chains. The antibody was used successfully for the immunohistochemical detection of reactive KDN epitopes in sections of paraffin embedded rat pancreas. Several controls verified the specificity of the immunohistochemical staining, thus providing the first demonstration of (8KDN2)
n
sequences in a mammalian tissue. The mAb.kdn8kdn can now be used to search further for glycoconjugates containing (8KDN2)
n
chains and will facilitate studies on their biosynthesis, intracellular localization and function. 相似文献
13.
An algorithm of learning in multilayer threshold nets without feedbacks is proposed. The net is. built of threshold elements with binary inputs. During a learning process each input vector x is accompanied by a teacher's decision ({1,...,M}). The pairs (x[n], [n]) appear in successive steps independently according to some unknown stationary distribution p(x,). The problem of learning of a threshold net has been decomposed to a series of problems of learning of the threshold elements. The proposed learning algorithm of the threshold elements has a perceptron-like form. It was proven that a decision rule of the threshold net stabilizes after a finite number of steps. For definite classes {p(x, )}
*
K
of distributions p(x,), an optimal decision rule stabilizes after a finite number of steps. These classes {p(x, )}
*
K
also contain distributions describing learning processes with perturbations. 相似文献
14.
Eric L. Charnov 《Evolutionary ecology》1995,9(3):288-291
Summary Offspring production over the adult lifespan (b/M whereb is the yearly fledgling or offspring production and 1/M is the mean adult lifespan) is an approximate invariant within both birds and mammals. The two taxa differ, however, in that mammals have bothM and b as invariants (b/M = b/M) while birds do not ( is the age at first breeding). Birds have a surprising cancellation in that bothM andb are
–0.25. 相似文献
15.
Zusammenfassung Serumproben von 1322 Blutspendern aus Hessen, 40 Familien mit 89 Kindern, 20 Mutter-Kind-Kombinationen und 268 Sera einer Bantupopulation aus Portugiesisch Angola wurden mit einer modifizierten Technik der Hochspannungs-Dünnschichtelektrophorese auf Agarosegel hinsichtlich des C3-Polymorphismus untersucht. Die Genfrequenzen für Weiße (C3S=0.779, C3F=0.215) und Neger (C3S=0.95, C3F=0,048) stimmen gut mit den Werten anderer Autoren überein. Insgesamt ließen sich bei Weißen 9 Phänotypen sicher abgrenzen, bei Negern 3. Familienstudien bestätigten den für die Allele C3S und C3F angenommenen Vererbungsmodus (autosomal codominant) ausnahmslos. Die Frage der Lagerungsstabilität des C3 wurde abschließend untersucht.
Investigations on C3-polymorphism ( 1c-Globulin)Gene frequencies and family studies in blood donors from Hessen and a Bantu population
Summary Serum samples of 1322 unrelated individuals from Hessen (Germany), 40 families with 89 children, 20 mother-child-combinations and 268 sera of a Bantu population from Angola were examined for C3 polymorphism using a modified technique of high voltage agarose gel electrophoresis. The gene frequencies for Caucasians (C3S=0.779, C3F=0.215) and negroes (C3S=0.95, CF=0.048) are in good accordance with those obtained by other authors. In total 9 different phenotypes were observed in Caucasians, 3 phenotypes in negroes. Family studies verify the supposed way of inheritance (autosomal codominant for C3S and C3F) without exception. Finally the problem of C3-inactivation by storage was investigated.相似文献
16.
Estimates of the extent of the discrimination against13CO2 during photosynthesis (A) on a global basis were made using gridded data sets of temperature, precipitation, elevation, humidity and vegetation type. Stomatal responses to leaf-to-air vapour mole fraction difference (D, leaf-to-air vapour pressure difference divided by atmospheric pressure) were first determined by a literature review and by assuming that stomatal behaviour results in the optimisation of plant water use in relation to carbon gain. Using monthly time steps, modelled stomatal responses toD were used to calculate the ratio of stomatal cavity to ambient CO2 mole fractions and then, in association with leaf internal conductances, to calculate A. Weighted according to gross primary productivity (GPP, annual net CO2 asimilation per unit ground area), estimated A for C3 biomes ranged from 12.9 for xerophytic woods and shrub to 19.6 for cool/cold deciduous forest, with an average value from C3 plants of 17.8. This is slightly less than the commonly used values of 18–20. For C4 plants the average modelled discrimination was 3.6, again slightly less than would be calculated from C4 plant dry matter carbon isotopic composition (yielding around 5). From our model we estimate that, on a global basis, 21% of GPP is by C4 plants and for the terrestrial biosphere as a whole we calculate an average isotope discrimination during photosynthesis of 14.8. There are large variations in A across the globe, the largest of which are associated with the precence or absence of C4 plants. Due to longitudinal variations in A, there are problems in using latitudinally averaged terrestrial carbon isotope discriminations to calculate the ratio of net oceanic to net terrestrial carbon fluxes. 相似文献
17.
Eneyskaya EV Kulminskaya AA Kalkkinen N Nifantiev NE Arbatskii NP Saenko AI Chepurnaya OV Arutyunyan AV Shabalin KA Neustroev KN 《Glycoconjugate journal》2001,18(10):827-834
An -L-fucosidase (E.C. 3.2.1.51) exhibiting a wide aglycon specificity expressed in ability of cleaving 1 6-, 1 3-, 1 4-, and 1 2-O-fucosyl bonds in fucosylated oligosaccharides, has been isolated from culture filtrate of Thermus sp. strain Y5. The -L-fucosidase hydrolyzes p-nitrophenyl -L-fucopyranoside with V
max of 12.0 ± 0.1 M/min/mg and K
m = 0.20 ± 0.05 mM and is able to cleave off about 90% of total L-fucose from pronase-treated fractions of fucosyl-containing glycoproteins and about 30% from the native glycoproteins. The purified enzyme is a tetramer with a molecular mass of 240 ± 10 kDa consisting of four identical subunits with a molecular mass of 61.0 ± 0.5 kDa. The N-terminal sequence showed homology to some -L-fucosidases from microbial and plant sources. Hydrolysis of p-nitrophenyl -L-fucopyranoside occurs with retention of the anomeric configuration. Transglycosylating activity of the -L-fucosidase was demonstrated in reactions with such acceptors as alcohols, N-acetylglucosamine and N-acetylgalactosamine while no transglycosylation products were observed in the reaction with p-nitrophenyl -L-fucopyranoside. The enzyme can be classified in glycosyl hydrolase family 29. 相似文献
18.
An open reading frame of the -subunit 1-205 residues (205) of human acetylcholine receptor (AchR) was amplified by PCR with pUC-AChR205 as the template and inserted into vector pMAL-c2X. The constructed pMAR205 was transferred into E. coli BL21 which were then grown in LB medium. The amount of soluble MBP-AChR205 protein reached about 25% of total soluble proteins from the cell lysate. Using amylose-affinity chromatography, about 35 mg MBP-AChR205 could be obtained from 1 l culture. Western blot analysis and ELISA showed that immunoreactivities of both MBP-AChR205 and AChR205 were similar to that of AChR -subunit from Torpedo.Revisions received 23 September 2004 相似文献
19.
Three allophycocyanin complexes were separated by gel electrophoresis, isoelectric focusing and ion exchange chromatography from a low molecular fraction (Mr 100–150000) of partially dissociated phycobilisomes of Mastigocladus laminosus: A. (APAP); B. (*AP2
AP2
AP*AP) · L
C
10
; and C. (*APAPBAP2
AP*AP) · L
C
10
. According to their fluorescence emission maximum at room temperature the complexes A., B. and C. are designated AP 660, AP 664 and AP 680. The different subunits of the AP complexes have apparent molecular weights of Mr 18500 *AP, 18200 APB, 18000 AP, 17000 AP and 16500 *AP. This hitherto unrecognized microheterogeneity within the AP subunits of complexes B. and C. of Mastigocladus laminosus phycobilisomes could also be demonstrated and confirmed with the two phycocyanin complexes PC 642 and PC 646. PC 642 is characterized by a L
R
11
linker polypeptide.Abbreviations AP
allophycocyanin
- PC
phycocyanin
- PEC
phycoerythrocyanin
- PE
phycoerythrin
- PAGE
polyacrylamide gel electrophoresis
- IEF
isoelectric focusing
- pI
isoelectric point
- Mr
apparent molecular weight
- TMED
tetramethylethylenediamine
- APS
ammonium persulphate
- SDS
sodium dodecylsulphate
- O.D.
optical density
A preliminary account of this work has been presented at the Embo Workshop on Oxygenic and Anoxygenic Electron Transport Systems in Cyanobacteria (Blue-green Algae) in Cape Sounion, Greece, 20–25 September 1987 相似文献
20.
Adam W. Dalziel Gert Lipka Babur Z. Chowdhry Julian M. Sturtevant David E. Schafer 《Molecular and cellular biochemistry》1984,63(1):83-91
Summary The B, or binding, subunit of cholera enterotoxin forms a pentameric ring structure in the intact toxin, and also when the subunit is isolated from the A subunit. The thermal denaturation of the B subunit ring was examined by differential scanning calorimetry in the presence and absence of ganglioside GM1, its natural receptor. In the absence of ganglioside an irreversible endotherm was observed with maximal excess apparent heat capacity, Cmax, at 74.6° C. When the ganglioside was added in increasing amounts, multiple transitions were observed at higher temperatures, the most prominent having a Cmax at 90.8° C. At high ganglioside concentrations, the 74.6° C transition was not observed. In addition to the thermodynamic results a model is proposed for the interaction of GM1 and B subunit pentamer. This model is derived independently of the calorimetric results (but is consistent with such data) and is based upon considerations of the geometry of the GM1 micelle-B subunit pentamer.Abbreviations Mr
molecular weight in daltons
- GM1
H3Neu-AcGgOse4Cer* = Gall 3Ga1NAc1 4Gal-[3 - 2NeuAc]1 4Glc1 1Cer (asterisked form follows the recommendations of the IUPACIUB Commission on Biochemical Nomenclature, Ref. 3)
- R
molar ratio of GM1 to B monomer
- DSC
differential scanning calorimetry
- Cmax
excess apparent heat capacity
- Cmax
maximal value of Cex
- tm
temperature (° C) at Cex = Cmax
- t1/2
peak width in °C at Cex = Cmax/2
- Hcal
calorimetric enthalpy
- C
p
d
van't Hoff enthalpy
- C
p
d
change in specific heat accompanying denaturation 相似文献