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A novel, tissue-restricted zinc finger protein (HF-1b) binds to the cardiac regulatory element (HF-1b/MEF-2) in the rat myosin light-chain 2 gene. 总被引:7,自引:4,他引:3
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H Zhu V T Nguyen A B Brown A Pourhosseini A V Garcia M van Bilsen K R Chien 《Molecular and cellular biology》1993,13(7):4432-4444
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A single MEF-2 site is a major positive regulatory element required for transcription of the muscle-specific subunit of the human phosphoglycerate mutase gene in skeletal and cardiac muscle cells.
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Y Nakatsuji K Hidaka S Tsujino Y Yamamoto T Mukai T Yanagihara T Kishimoto S Sakoda 《Molecular and cellular biology》1992,12(10):4384-4390
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The basic helix-loop-helix protein upstream stimulating factor regulates the cardiac ventricular myosin light-chain 2 gene via independent cis regulatory elements. 总被引:5,自引:4,他引:1
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S Navankasattusas M Sawadogo M van Bilsen C V Dang K R Chien 《Molecular and cellular biology》1994,14(11):7331-7339
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S Goswami P Qasba S Ghatpande S Carleton A K Deshpande M Baig M A Siddiqui 《Molecular and cellular biology》1994,14(8):5130-5138
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Positive regulatory elements (HF-1a and HF-1b) and a novel negative regulatory element (HF-3) mediate ventricular muscle-specific expression of myosin light-chain 2-luciferase fusion genes in transgenic mice. 总被引:5,自引:3,他引:2
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The cardiac myosin light-chain 2v (MLC-2v) gene has served as a model system to identify the pathways which restrict the expression of cardiac muscle genes to particular chambers of the heart during cardiogenesis. To identify the critical cis regulatory elements which mediate ventricular chamber-specific expression of the MLC-2v gene in the in vivo context, a series of transgenic mice which harbor mutations in putative MLC-2 cis regulatory elements in a 250-bp MLC-2-luciferase fusion gene which is expressed in a ventricular chamber-specific fashion in transgenic mice were generated. These studies demonstrate that both components of HF-1 (HF-1a and HF-1b/MEF-2) are required to maintain ventricular chamber-specific expression and function as positive regulatory elements. Mutations in another conserved element (HF-2) are without statistically significant effect on ventricular chamber expression. Transgenics harboring mutations in the E-box site also displayed significant upregulation of reporter activity in the soleus, gastrocnemius, and uterus, with a borderline effect on expression in liver. Mutations in another conserved element (HF-3) result in a marked (> 75-fold) upregulation of the luciferase reporter activity in the soleus muscle of multiple independent or transgenic founders. Since the HF-3 mutations appeared to have only a marginal effect on luciferase reporter activity in liver tissue, HF-3 appears to function as a novel negative regulatory element to primarily suppress expression in muscle tissues. Thus, a combination of positive (HF-1a/HF-1b) and negative (E-box and HF-3) regulatory elements appear to be required to maintain ventricular chamber-specific expression in the in vivo context. 相似文献
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Control of cardiac-specific transcription by p300 through myocyte enhancer factor-2D 总被引:1,自引:0,他引:1
Slepak TI Webster KA Zang J Prentice H O'Dowd A Hicks MN Bishopric NH 《The Journal of biological chemistry》2001,276(10):7575-7585
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