在不断改革中与时俱进——写在《生物化学与生物物理进展》创刊40周年

<正>《生物化学与生物物理进展》(以下简称《进展》)由中国科学院生物物理研究所创办于1974年(后与中国生物物理学会合办),迄今已届40周年.《进展》在文革尚未结束的特殊艰难时期诞生,随后与我国的社会变革和科学技术进步同行,在不断改革中发展,已经成为一份具备现代科技期刊的基本要素,被我国生物学界广为认知,并具有一定国际影响力的知名学术期刊.《进展》作为发表与记录科研成果的载体和传播科技知识的媒介,从创刊至今,刊物容量增加了近10倍,发表了来自全国近500多个单位的6381篇论     

不断改革 与时俱进 努力办好《生物化学与生物物理进展》——写在《生物化学与生物物理进展》创刊30周年暨第五届编委会成立

《生物化学与生物物理进展》(以下简称《进展》)创刊于1974年,至今已30周年．恰逢此时,以中青年为主体的新一届编委会也正式成立．这预示着《进展》正在进人一个历史发展的新阶段,值得热烈祝贺．     

与国家科技进步同行 在不断改革中发展 《生物化学与生物物理进展》四十年

<正>《生物化学与生物物理进展》创刊于1974年。其时,"文革"尚未结束,我国的科学技术凋零四处,百废待兴。在封闭10年之后,科技工作开始提上曰程,酝酿重新启动,科研和教学人员渴求新的科学技术知识,渴望了解国际科学技术的状况。《生物化学与生物物理进展》的创刊,正是适应于这样的时代背景,服务于这样的时代需求。创刊之初,《生物化学与生物物理进展》编辑人员积极深入教学和科研单位,了解实际需求,不断改进栏目设置、丰富刊物内容。刊物很快受到科研人员的欢迎和好评。1977年,刊物由创刊之初的季刊改版为双月刊。     

似鳡属的系统地位及种的分化(鲤形目:鲤科)

似鳡属(Luciocyprinus Vaillant)是鲤科鱼类中属于鲃系(陈湘粦等,1984)的一个属,下咽齿3行,臀鳍无硬刺,具分枝鳍条5根。过去只记载一个种,外形很像鳡鱼,是由于摄食习性相同而导致的趋同现象(林人端,1981),故称似鳡,在广西俗称墨线鳡,云南俗称杆条鱼。分布于西江水系,包括云南中南部的湖泊,与鳡鱼分布于同一水系,但各占一定的水域,互不重叠。 关于似鳡的拉丁名称,惯用Fustis vivus Lin,是林书颜1932年定的新属新种,     

Isolation and properties of ovine [1-pyroglutamic acid]-β-lipoprotein

A new form of ovine β-lipotropin has been isolated by partition chromatography on agarose gel. All the evidence is consistent with the conclusion that its structure is identical to that of ovine β-lipotropin with the exception that the glutamic acid residue in position 1 was replaced by a pyroglutamic acid residue. Its lipolytic activity in isolated rabbit fat cells was about one-half that of β-lipotropin.     

齐齐哈尔东郊东方白鹳繁殖情况的调查与观察

1969～1986年期间,曾在1969～1970,1978～1979,1980～1984,和1986共10个年分里每年有一对东方白鹳(Ciconia ciconia boyciana Swinhoe)先后在7个地点营巢繁殖,本文总结了10次营巢与繁殖情况,并对其繁殖过程机率作了时间空间分析。     

Improved strength of silk fibers in Bombyx mori trimolters induced by an anti-juvenile hormone compound

Background

Bombyx mori silk fibers with thin diameters have advantages of lightness and crease-resistance. Many studies have used anti-juvenile hormones to induce trimolters in order to generate thin silk; however, there has been comparatively little analysis of the morphology, structure and mechanical properties of trimolter silk.

Variation in torpidity of diapause in freshwater cyclopoid copepods

By successively filtering off animals emerging from mud cores taken to the laboratory, the degree of torpidity within the same species was found to vary considerably both time and space. Variation in time from the start of dormancy follows a pattern of diapause development similar to that of insect diapause. In some localities dormancy is deep and prolonged, while in others it is less pronounced and lasts for a shorter period. The depth of torpidity during winter seems to be correlated to an induction early in the year. The intensity of diapause varies along the slope of the localities and down into the bottom mud. In some cases high temperatures seem to induce a deeper torpidity.     

Distinguishing specific and nonspecific interdomain interactions in multidomain proteins

Multidomain proteins account for over two-thirds of the eukaryotic genome. Although there have been extensive studies into the biophysical properties of isolated domains, few have investigated how the domains interact. Spectrin is a well-characterized multidomain protein with domains linked in tandem array by contiguous helices. Several of these domains have been shown to be stabilized by their neighbors. Until now, this stabilization has been attributed to specific interactions between the natural neighbors, however we have recently observed that nonnatural neighboring domains can also induce a significant amount of stabilization. Here we investigate this nonnative stabilizing effect. We created spectrin-titin domain pairs of both spectrin R16 and R17 with a single titin I27 domain at either the N- or the C-terminus and found that spectrin domains are significantly stabilized, through slowed unfolding, by nonnative interactions at the C-terminus only. Of particular importance, we show that specific interactions between natural folded neighbors at either terminus confer even greater stability by additionally increasing the folding rate constants. We demonstrate that it is possible to distinguish between natural stabilizing interactions and nonspecific stabilizing effects through examination of the kinetics of well chosen mutant proteins. This work adds to the complexity of studying multidomain proteins.     

Discontinuous DNA replication: accumulation of Simian virus 40 DNA at specific stages in its replication.

The number of proline residues in a protein should have very marked consequences for the rates of protein unfolding and refolding according to the model proposed by Brandts et al. (1975). Kinetic simulations of this model indicate that the half-time for refolding of a polypeptide chain with 20 proline residues should be greater than 10 minutes and should increase by about an order of magnitude for each additional 10 proline residues. Various means are considered by which the rate of protein folding in vivo and in vitro might be increased.     

Temperature regulation during centrifugal elutriation and its effect on cell separation

Centrifugal elutriation appears to be a promising method for cell separation. The quality of the separation may be limited by the control of temperature within the separation chamber, which affects the fluid viscosity and rotor speed. The factors affecting the temperature regulation have been re-examined. At flow rates between 10 and 40 mL/min the temperature within the chamber was primarily dependent on the temperature of the fluid flowing into the rotor. Increases in the temperature of the fluid while it flowed through the rotor were observed and were greater at higher rotor speeds and lower flow rates. This heating, caused by friction at the rotating seal, could raise the fluid temperature within the chamber by as much as 6°C. Fluctuations in the temperature of the centrifuge produced temperature variations of only 0.3°C in the fluid in the elutriation chamber. Small increases in the rate of elutriation of cells, concomitant with centrifuge cooling and speed fluctuations, were detected by optical density measurements. However, neither the modal volume nor coefficient of variation of the collected cells were affected.